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J Biol Chem, Vol. 274, Issue 49, 34507-34510, December 3, 1999

MINIREVIEW
Cortical Actin Organization: Lessons from ERM (Ezrin/Radixin/Moesin) Proteins^*

Sachiko Tsukita^§¶ and Shigenobu Yonemura^§

From the  College of Medical Technology, Kyoto University, Sakyo-ku, Kyoto 606, Japan and ^§ Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan

	INTRODUCTION

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

In recent years to clarify the molecular mechanism of dynamic organization of the cortical actin filaments, which is important not only for the determination of cell-surface structures but also for the functions of integral membrane proteins themselves, various types of submembrane proteins involved in cortical actin filament/plasma membrane interaction have been intensively studied.

In this minireview, we focus on ezrin/radixin/moesin (ERM)¹ proteins, which are general cross-linkers between cortical actin filaments and plasma membranes and are involved in the formation of microvilli, cell adhesion sites, ruffling membranes, and cleavage furrows. ERM proteins have attracted a great deal of interest because their functions have been shown to be regulated by the Rho signaling pathway (for recent reviews, see Refs. 1-7).

	Structure of ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

The ERM family consists of three closely related proteins, ezrin, radixin, and moesin (ERM proteins) (8) (Fig. 1). Ezrin (~82 kDa) was first isolated from chicken intestinal brush borders as a component of microvilli (9). Molecular cloning revealed that ezrin was identical to cytovillin, which was enriched in microvilli of human placental syncytiotrophoblasts (10, 11). Radixin (~80 kDa) was isolated from rat liver as a component of adherens junctions (12). Moesin (~75 kDa) was isolated from bovine uterus abundant in smooth muscle cells as a heparin-binding protein (13). Homologues for ERM proteins have been found from Caenorhabditis elegans to human, although the number of family members appears to vary from one to three depending on species (2).

View larger version (32K): [in this window] [in a new window]   Fig. 1.   ERM family members (ezrin, radixin, moesin), merlin/ schwannomin, and band 4.1 protein. ERM proteins consist of three domains: a globular N-terminal membrane-binding domain (FERM domain or N-ERMAD), followed by an extended -helical domain and a positively charged C-terminal actin-binding domain (C-ERMAD). Proteins as well as PIP2 that bind to ERM proteins are listed at the top (for details, see "Structure of ERM Proteins"). In some papers on ERM proteins the amino acid residues are numbered from proline 1, because methionine 1 is posttranslationally removed in human ezrin and moesin (10, 15). In this review, however, they are numbered from methionine 1. The percentage sequence identity with ezrin in each domain is indicated at the amino acid sequence level.

The sequences of their N-terminal halves are highly conserved (~85% identity) and similar to the N-terminal half of human erythroid band 4.1 protein (~78 kDa), indicating that the ERM family is included in the band 4.1 superfamily that contains merlin/schwannomin (a tumor suppressor molecule for neurofibromatosis type II), talin, PTP-H1, and PTP-MEG. Among these, merlin (isoforms I-III)(~70 kDa) is fairly similar to ERM proteins (~60% identity). The sequence, which is conserved among the members of the band 4.1 superfamily and referred to as the FERM (4.1 and ERM) domain, is a membrane-binding site in band 4.1 protein, and similar sequences have recently been found in the central portion of PTP-BAS and the C-terminal domain of myosin VIIA. In ERM proteins, the N-terminal FERM domain is followed by an extended -helical domain and a charged C-terminal domain, which includes a consensus sequence motif for actin binding. Thus, from their structure, ERM proteins have been suggested to function as cross-linkers between actin filaments and plasma membranes (1-7).

	Subcellular Distribution and Functions of ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

Immunoblotting analysis and immunofluorescence microscopy revealed that in most cultured cells all ERM proteins are co-expressed and co-localized but that in organs their expression and distribution pattern appear to be regulated in a cell type-specific manner (1-7). Immunofluorescence studies of cultured fibroblasts and epithelial cells have revealed that ERM proteins are co-expressed and co-concentrated at cell-surface structures such as microvilli, filopodia, uropods, ruffling membranes, retraction fibers, and cell adhesion sites where actin filaments are associated with plasma membranes (8, 14-17) (Fig. 2). ERM proteins are also concentrated specifically at cleavage furrows in dividing cells (14) but not along cytoplasmic actin filaments such as stress fibers, in contrast to filamin and -actinin, which are concentrated in both sites (18). Suppression of the expression of all ERM proteins with antisense oligonucleotides in cultured fibroblasts/epithelial cells destroyed microvillus formation as well as cell-to-cell/cell-to-substrate adhesion (19). Similarly, in cultured neurons that contain mainly radixin and moesin, antisense oligonucleotides of radixin and moesin severely affected the morphology, motility, and process formation of growth cones (20). Specific ezrin ablation by MicroCALI (chromatophore-assisted laser irradiation) blocked membrane ruffling and motility (21). Furthermore, overproduction of full-length ERM proteins appeared to enhance cell adhesion, whereas that of their C-terminal halves perturbed the cell-surface morphology and inhibited cytokinesis (22, 23). These findings suggested that ERM proteins were involved in the formation and/or maintenance of cortical actin organization through their cross-linking activity between actin filaments and plasma membranes.

View larger version (65K): [in this window] [in a new window]   Fig. 2.   Immunofluorescence microscopy of cultured baby hamster kidney cells with anti-ERM polyclonal antibody. ERM proteins are co-concentrated at cell-surface structures such as microvilli (Mv), cell-cell adhesion sites (C-C), and cleavage furrows (CF) where actin filaments are associated with plasma membranes. Bar, 20 µm.

Extensive functional analyses suggested the possible functional redundancy of ERM proteins at least at the cellular level. Recently, moesin-deficient mice were generated by gene targeting, and they appeared normal without any compensatory up-regulation of ezrin or radixin (24). Therefore, also at the whole body level ERM proteins appear to be functionally redundant, although ERM proteins are not necessarily co-localized and co-expressed at the organ level. Targeted disruption of ezrin and radixin genes will allow clarification of this redundancy problem in the near future.

	Actin and Membrane Binding of ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

The C-terminal halves of ERM proteins bind to F-actin through their major actin-binding sites, the C-terminal 34 amino acids, which are highly conserved among these proteins (25). In addition to this domain, two more actin-binding domains have recently been identified in their N-terminal and middle regions, which bind to F-actin and both F- and G-actin, respectively (26). Although the physiological relevance of these newly identified actin-binding sites in ERM proteins is not clear at present, the mode of association of actin filaments with ERM proteins does not appear to be simple. G-actin binding affinity in their middle regions would explain the actin barbed end-capping activity of ERM proteins, which was detected in radixin at low ionic strength (12).

On the other hand, the N-terminal halves of ERM proteins were reported to directly bind to the cytoplasmic domains of CD44 (27, 28) and other integral membrane proteins such as ICAM-1, -2, and -3 and CD43, which were co-localized with ERM proteins in vivo (17, 27-32). Although the cytoplasmic domains of these integral membrane proteins have no shared sequences, their juxtamembrane positively charged amino acid clusters are thought to be responsible for their binding to ERM proteins (32, 33). This direct binding of ERM proteins with integral membrane proteins was shown to be essential for cell-surface morphogenesis such as microvillus formation (34, 35). Ezrin effected the function of ICAM-2 in thymoma cells for being targeted by natural killer cells (29).

The mechanism of indirect binding of ERM proteins to integral membrane proteins has also been reported. EBP-50 (ERM-binding phosphoprotein of 50 kDa) was identified as a cytoplasmic protein, the C-terminal region of which binds to the N-terminal half of ezrin (36). Sequence analyses revealed that EBP-50 is identical to a Na⁺/H⁺ exchanger regulatory factor (NHE-RF). This NHE-RF and its isoform, E3KARP, bear two PDZ domains, which were shown to directly bind to the C terminus of NHE3 (36, 37). Thus, NHE-RF and E3KARP can function as adapters between NHE3 and ezrin (38). Interestingly, because NHE-RF regulates the NHE3 function in a protein kinase A (PKA)-dependent manner and because ezrin specifically binds to the RII subunit of PKA (38), ezrin appears to play an important role in recruiting PKA to the NHE3·NHE-RF complex to regulate the function of NHE3. Furthermore, NHE-RF and E3KARP were found to also be associated with other integral membrane proteins such as the ₂-adrenergic receptor and the cystic fibrosis transmembrane conductance regulator (39, 40), which are not always co-localized with ERM proteins (41). The physiological relevance of the existence of two mechanisms of binding of ERM proteins to integral membrane proteins, direct and indirect, is an interesting subject for future study.

	Intramolecular Interdomain Interaction between N- and C-terminal Halves of ERM Proteins: Molecular Mechanism for Inactivation of ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

Given that the cortical actin filaments are dynamically organized in response to various signals, the cross-linking activity of ERM proteins between actin filaments and plasma membranes is expected to be dynamically regulated. Indeed, the pioneering work by Bretscher (42) or Hanzel et al. (43) showed that EGF treatment of A431 cells or the secretion-stimulation of parietal cells rapidly recruited substantial amounts of ERM proteins to the cortical actin layer with concomitant phosphorylation of ERM proteins. When conventionally cultured cells were homogenized and centrifuged in physiological saline, ERM proteins were partitioned almost equally into the soluble and insoluble fractions (44). These findings suggested that there are active (insoluble) and inactive (soluble) forms of ERM proteins in terms of their cross-linking activity inside cells.

Evidence has accumulated in vitro and in vivo that the N- and C-terminal halves of ERM proteins mutually interact intramolecularly and suppress their actin filament and membrane binding activities, respectively (45-47). Recently, it was shown that when two amino acid residues were deleted from the C-terminal end of ezrin, which do not correspond to the EBP-50-binding domain, the interdomain interaction was affected, allowing ezrin to directly interact with EBP-50 (37). These findings indicated that conformational masking by intramolecular interdomain interaction is the molecular mechanism behind the inactivation of ERM proteins.

The region involved in the interdomain interaction was narrowed down to residues 1-297 and 480-586 (1-296 and 479-585 when methionine 1 is posttranslationally removed) as exemplified in ezrin, and these regions are called N- and C-ERMADs (ERM-association domains), respectively (46). Initially, these N- and C-ERMADs were thought to be responsible for oligomerization of ERM proteins, i.e. intermolecular interaction of ERM proteins, but it has been suggested that they are also important for intramolecular interaction (6, 7). Furthermore, yeast two-hybrid analyses identified several middle regions between N- and C-ERMADs that interact with N- and C-ERMADs (48). Although the molecular mechanism and physiological relevance of dimerization and oligomerization of ERM proteins remain elusive, it is now accepted that the intramolecular mutual suppression mechanism keeps ERM proteins in an inactive state and that some activation signal may release this suppression to activate ERM proteins inside cells.

	Phosphorylation and PIP2 Binding as Activation Signals of ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

To date, two molecular events have been shown to generate and/or maintain the active form of ERM proteins in vitro: phosphorylation of their C-terminal threonine residue and PIP₂ binding to their N-terminal domains. The phosphorylation of ERM proteins, especially ezrin, has been examined in detail. EGF stimulation induced tyrosine and serine phosphorylation of ezrin in A431 cells with concomitant translocation from the cytoplasm to the cortical actin layer (42). The tyrosine residues that were phosphorylated in A431 cells were shown to be Tyr-146 and Tyr-354 (Tyr-145 and Tyr-353 when methionine 1 is posttranslationally removed) in ezrin, the former of which was conserved in radixin and moesin (49). ERM proteins were also reported to be heavily tyrosine-phosphorylated by v-Src and hepatocyte growth factor (50, 51). However, the substitution of Tyr-146 with phenylalanine in ezrin showed some effects on cell motility but did not affect the cortical localization of ezrin induced by hepatocyte growth factor. Thus, the direct activation of ERM proteins by tyrosine phosphorylation is unlikely.

Secretion/stimulation in gastric parietal cells was reported to induce serine/threonine phosphorylation of ezrin (43). In platelets thrombin activation induced the phosphorylation of moesin at a specific C-terminal threonine residue (Thr-558) (52), causing filopodia formation. This site was effectively phosphorylated in vitro by ROK/ROCK-II/Rho-kinase in radixin (Thr-564) and by PKC- (53, 54). However, it is likely that ROCK kinases do not phosphorylate ERM proteins in vivo (55). In vitro functional analyses suggested that the C-terminal threonine phosphorylation maintains ERM proteins in the active state by suppressing the intramolecular interaction (53). Immunofluorescence microscopy with monoclonal antibodies specific for C-terminal threonine-phosphorylated ERM proteins revealed that ERM proteins localized beneath plasma membranes were actually phosphorylated at the C-terminal threonine in vivo (53, 56). Taken all together, it seems that the threonine phosphorylation just maintains the activated ERM proteins.

Another candidate for the activation signal for ERM proteins is PIP₂, which has been shown to directly bind to the N-terminal halves of ERM proteins in vitro (28, 57). Recently, it has been shown that PIP₂ is a key factor for the activation of ERM proteins in vivo (55).

	ERM Proteins Downstream as Well as Upstream of Rho

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

The question has thus arisen as to the identities of the upstream factors required for activation of ERM proteins. Rho, one of the small GTP-binding proteins, is now considered to be a general regulator of actin-based cytoskeletal organization. To date, in vitro as well as in vivo analyses have suggested an intimate relationship between the Rho signaling pathway and activation of ERM proteins. First, the binding ability of ERM proteins to the cytoplasmic domain of CD44 in crude cell homogenate was reported to be enhanced by activation of Rho (28). In semi-permeabilized Swiss 3T3 cells, at least one of the ERM proteins was shown to be required for Rho-dependent formation of stress fibers and focal contacts (58). Furthermore, transfection of the constitutively active mutant of RhoA (V14RhoA), but not that of Rac1 or Cdc42, induced microvillus formation to which ERM proteins were recruited (55, 59). Thus, it is now accepted that when Rho is activated in vivo, ERM proteins in the cytoplasm are activated and recruited to plasma membranes to form microvilli. Although ERM proteins are also suggested to be located downstream of Rac (58), the activation of ERM proteins to form microvilli specifically depends on Rho but not on Rac.

Rho has been reported to activate several serine/threonine kinases such as ROK/ROCK-II/Rho-kinase, ROK/ROCK-I, citron kinase, protein kinase N, and protein kinase C1 (58). Immunoblotting with a monoclonal antibody specific for C-terminal threonine-phosphorylated ERM proteins revealed that in serum-starved Swiss 3T3 cells Rho activation by lysophosphatidic acid stimulation increased the levels of the C-terminal threonine phosphorylation of ERM proteins (53). Rho-kinase, which effectively phosphorylates the C-terminal threonines of ERM proteins in vitro, is not responsible for this Rho-dependent threonine phosphorylation of ERM proteins in vivo.

PIP₂-producing phosphatidylinositol 4-phosphate 5-kinase (PI4P5K) has also been reported to be a direct Rho effector (for a review, see Ref. 60). Because activation of dormant ERM proteins was induced by PIP₂ in vivo as well as in vitro (28, 55), one possible pathway for the activation of ERM proteins is as follows. Rho may activate PI4P5K, which in turn increases the amount of PIP₂. PIP₂ then activates ERM proteins by inhibiting their interdomain interaction, which allows phosphorylation of their C-terminal threonine residue by unidentified kinases. The C-terminally threonine-phosphorylated ERM proteins are stabilized as activated forms, which function as actin filament/plasma membrane cross-linkers to form microvilli (Fig. 3).

View larger version (31K): [in this window] [in a new window]   Fig. 3.   Activation pathway of ERM proteins. Rho activates PI4P5K, which increases the amount of PIP2. PIP2 then activates ERM proteins by inhibiting their interdomain interaction, which allows phosphorylation of their C-terminal threonine residue by some kinases. The C-terminally threonine-phosphorylated ERM proteins are stabilized at the activated forms, which function as actin filament/plasma membrane cross-linkers to form microvilli. Activated ERM proteins are associated directly with the adhesion molecules such as CD44 and ICAM-1, -2, and -3 and indirectly with other integral membrane proteins such as NHE3 through EBP-50/NHE-RF. Activated ERM proteins also bind to Rho-GDI at their N-terminal halves, suppressing GDI activity of Rho-GDI to release GDP-Rho, which is activated to GTP-Rho. This GTP-Rho can be used to activate ERM proteins just beneath the plasma membranes, providing a positive feedback pathway.

On the other hand, immunoprecipitation experiments identified Rho-GDI (GDP dissociation inhibitor) in the CD44-ERM protein complex (28). An in vitro binding study then revealed that active but not inactive forms of ERM proteins directly bound to Rho-GDI at their N-terminal halves (61). Interestingly, this binding of ERM proteins suppressed GDI activity of Rho-GDI, i.e. GDP-Rho was released from Rho-GDI, followed by activation as GTP-Rho possibly through Dbl (GDP/GTP exchange protein) (61, 62). These findings suggested that ERM proteins, once activated, can activate Rho, which again activates ERM proteins as a positive feedback system. In this sense, ERM proteins are located not only downstream but also upstream of Rho.

	Inactivation Signals for ERM Proteins

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

Because native monomers of ERM proteins have a tendency to inactivate themselves by the interdomain interaction, the down-regulation of activation signals may inactivate ERM proteins inside cells. For example, at the initial phase of apoptosis, ERM proteins were dephosphorylated and inactivated, resulting in their cytoplasmic translocation with concomitant microvillar breakdown (44). In this process, the C-terminal threonine was confirmed to be dephosphorylated by the monoclonal antibody specific for C-terminal threonine-phosphorylated ERM proteins.² Recently, myosin light chain phosphatase was shown to bind to moesin through its myosin-binding subunit in vitro, although its physiological relevance remains unclear (63). On the other hand, proteolysis could be another method of inactivation of ERM proteins because ezrin is a good substrate for calpain in vitro (6, 7). Although evidence is still lacking, on and off activation and/or inactivation signals and their balance may regulate the function of ERM proteins.

	ERM Proteins and Merlin

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

Some, but not all, of the characteristics of ERM proteins appear to be shared by merlin. The subcellular localization of ERM proteins was similar to that of merlin in fibroblasts (microvilli and ruffling membranes) but different in epithelial cells; merlin, but not ERM proteins, was concentrated at lateral membranes together with E-cadherin (64). The N-terminal half of merlin bound to the cytoplasmic domains of CD44 (65) and NHE-RF (66) as well as Rho-GDI in vitro (64). Merlin has two major alternatively spliced isoforms, I and II, which differ at their C-terminal ends (67). Although neither of these C-terminal ends shows any similarity to those of ERM proteins, the major actin-binding domains, merlin has been reported to bind to actin filaments at its middle region (68). Interdomain interaction between the N- and C-terminal halves has been suggested in isoform-I, but the interaction does not appear to affect its binding affinity to actin filaments or to NHE-RF (66, 68). No interdomain interaction has been detected in isoform-II (69).

At present, there are three distinct possible explanations for the sequence similarity between ERM proteins and merlin. First, it is possible that merlin functions in cells by competing for shared binding partners such as CD44, NHE-RF, and Rho-GDI. However, because the molar ratio of endogenous merlin/ERM was calculated to be 0.05-0.15 in cultured fibroblasts and epithelial cells, the ERM-merlin competition is not likely in vivo. Second, merlin would be functionally redundant for ERM proteins. Data from the suppression experiments of ERM proteins by antisense oligonucleotides discussed above are inconsistent with this explanation. Furthermore, this explanation was not supported by the observation that merlin-deficient mice showed an embryonic lethal phenotype (70). Third, it is also possible that merlin shows sequence similarity to ERM proteins because both are involved in Rho signaling pathways; for example, both bind to Rho-GDI. If this is the case, loss of function mutations of merlin may induce tumorigenesis through some disturbance in the Rho signaling pathway. In this connection, it should be pointed out that neurofibromin, which is responsible for neurofibromatosis type I, shows GTPase-activating protein activity for Ras (71). It is reasonable to speculate that both neurofibromatosis types I and II are caused by some dysregulation of small GTP-binding protein-dependent signaling pathways.

	Perspective

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

In the past decade, it has been established that ERM proteins function as general cross-linkers in the cortical layer, coupled with signal transduction pathways such as Rho signaling. Because ERM proteins are expressed almost ubiquitously, this cross-linking system would be involved in various cellular events in various types of cells. Thus, in the coming decade, ERM proteins will attract increasing interest in many fields from not only biological but also medical researchers. For example, in the immune system ERM proteins are thought to play an important role in cell recognition of T lymphocytes by producing uropods (17, 29).

It will also be interesting to study the interactions between ERM proteins and microtubules. Radixin was characterized as a marginal microtubule band-associated protein in nucleated erythrocytes (72), and in activated T-lymphocytes tubulin was co-concentrated at the uropods together with ERM proteins (17). Furthermore, it was also shown that ERM proteins have some homology with Tea 1, which is localized on the ends of microtubules and is critical for polarization in yeast (73). Further studies of the ERM proteins-microtubule interaction will provide new insight into the physiological functions of ERM proteins as well as the cortical actin layer.

	FOOTNOTES

^* This minireview will be reprinted in the 1999 Minireview Compendium, which will be available in December, 1999. This is the fourth article of four in the "Proteins That Regulate Dynamic Actin Remodeling in Response to Membrane Signaling Minireview Series."

^¶ To whom correspondence should be addressed. Tel.: 81-75-753-4373; Fax: 81-75-753-4660; E-mail: atsukita@mfour.med.kyoto-u.ac.jp.

² Sa. Tsukita, unpublished data.

	ABBREVIATIONS

The abbreviations used are: ERM, ezrin/radixin/moesin; EBP-50, ERM-binding phosphoprotein of 50 kDa; PTP, protein-tyrosine phosphatase; NHE-RF, Na⁺/H⁺ exchanger regulatory factor; PKA, protein kinase A; ERMAD, ERM-association domain; PIP₂, phosphatidylinositol 4,5-bisphosphate; GTPS, guanosine 5'-O-(3-thio)triphosphate; PI4P5K, phosphatidylinositol 4-phosphate 5-kinase; GDI, GDP dissociation inhibitor.

	REFERENCES

TOP INTRODUCTION Structure of ERM Proteins Subcellular Distribution and... Actin and Membrane Binding... Intramolecular Interdomain... Phosphorylation and PIP2... ERM Proteins Downstream as... Inactivation Signals for ERM... ERM Proteins and Merlin Perspective REFERENCES

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				C. Csortos, I. Kolosova, and A. D. Verin Regulation of vascular endothelial cell barrier function and cytoskeleton structure by protein phosphatases of the PPP family Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L843 - L854. [Abstract] [Full Text] [PDF]

				Y. Furutani, H. Matsuno, M. Kawasaki, T. Sasaki, K. Mori, and Y. Yoshihara Interaction between Telencephalin and ERM Family Proteins Mediates Dendritic Filopodia Formation J. Neurosci., August 15, 2007; 27(33): 8866 - 8876. [Abstract] [Full Text] [PDF]

				S.-i. Terawaki, K. Kitano, and T. Hakoshima Structural Basis for Type II Membrane Protein Binding by ERM Proteins Revealed by the Radixin-neutral Endopeptidase 24.11 (NEP) Complex J. Biol. Chem., July 6, 2007; 282(27): 19854 - 19862. [Abstract] [Full Text] [PDF]

				F. Besse, S. Mertel, R. J. Kittel, C. Wichmann, T. M. Rasse, S. J. Sigrist, and A. Ephrussi The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses J. Cell Biol., June 21, 2007; 177(5): 843 - 855. [Abstract] [Full Text] [PDF]

				J. D. Lee, N. F. Silva-Gagliardi, U. Tepass, C. J. McGlade, and K. V. Anderson The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo Development, June 1, 2007; 134(11): 2007 - 2016. [Abstract] [Full Text] [PDF]

				S. Chateauvieux, J.-L. Ichante, B. Delorme, V. Frouin, G. Pietu, A. Langonne, N. Gallay, L. Sensebe, M. T. Martin, K. A. Moore, et al. Molecular profile of mouse stromal mesenchymal stem cells Physiol Genomics, April 24, 2007; 29(2): 128 - 138. [Abstract] [Full Text] [PDF]

				G. Tamma, G. Procino, M. Svelto, and G. Valenti Hypotonicity causes actin reorganization and recruitment of the actin-binding ERM protein moesin in membrane protrusions in collecting duct principal cells Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1476 - C1484. [Abstract] [Full Text] [PDF]

				A. Hatzoglou, I. Ader, A. Splingard, J. Flanders, E. Saade, I. Leroy, S. Traver, S. Aresta, and J. de Gunzburg Gem Associates with Ezrin and Acts via the Rho-GAP Protein Gmip to Down-Regulate the Rho Pathway Mol. Biol. Cell, April 1, 2007; 18(4): 1242 - 1252. [Abstract] [Full Text] [PDF]

				H. Takamatsu, X. Feng, T. Chuhjo, X. Lu, C. Sugimori, K. Okawa, M. Yamamoto, S. Iseki, and S. Nakao Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia Blood, March 15, 2007; 109(6): 2514 - 2520. [Abstract] [Full Text] [PDF]

				A. J. LaGier, S. H. Yoo, E. C. Alfonso, S. Meiners, and M. E. Fini Inhibition of Human Corneal Epithelial Production of Fibrotic Mediator TGF-{beta}2 by Basement Membrane-Like Extracellular Matrix Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1061 - 1071. [Abstract] [Full Text] [PDF]

				H. Chiba, N. Sakai, M. Murata, M. Osanai, T. Ninomiya, T. Kojima, and N. Sawada The nuclear receptor hepatocyte nuclear factor 4{alpha} acts as a morphogen to induce the formation of microvilli J. Cell Biol., December 18, 2006; 175(6): 971 - 980. [Abstract] [Full Text] [PDF]

				Y. Nukada, N. Okamoto, S. Konakahara, K. Tezuka, K. Ohashi, K. Mizuno, and T. Tsuji AILIM/ICOS-mediated elongation of activated T cells is regulated by both the PI3-kinase/Akt and Rho family cascade Int. Immunol., December 1, 2006; 18(12): 1815 - 1824. [Abstract] [Full Text] [PDF]

				E. Martin-Villar, D. Megias, S. Castel, M. M. Yurrita, S. Vilaro, and M. Quintanilla Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition J. Cell Sci., November 1, 2006; 119(21): 4541 - 4553. [Abstract] [Full Text] [PDF]

				T. Simoncini, C. Scorticati, P. Mannella, A. Fadiel, M. S. Giretti, X.-D. Fu, C. Baldacci, S. Garibaldi, A. Caruso, L. Fornari, et al. Estrogen Receptor {alpha} Interacts with G{alpha}13 to Drive Actin Remodeling and Endothelial Cell Migration via the RhoA/Rho Kinase/Moesin Pathway Mol. Endocrinol., August 1, 2006; 20(8): 1756 - 1771. [Abstract] [Full Text] [PDF]

				M. Sala-Valdes, A. Ursa, S. Charrin, E. Rubinstein, M. E. Hemler, F. Sanchez-Madrid, and M. Yanez-Mo EWI-2 and EWI-F Link the Tetraspanin Web to the Actin Cytoskeleton through Their Direct Association with Ezrin-Radixin-Moesin Proteins J. Biol. Chem., July 14, 2006; 281(28): 19665 - 19675. [Abstract] [Full Text] [PDF]

				C. M. Grimsley, M. Lu, L. B. Haney, J. M. Kinchen, and K. S. Ravichandran Characterization of a Novel Interaction between ELMO1 and ERM Proteins J. Biol. Chem., March 3, 2006; 281(9): 5928 - 5937. [Abstract] [Full Text] [PDF]

				D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]

				B. Ratnikov, C. Ptak, J. Han, J. Shabanowitz, D. F. Hunt, and M. H. Ginsberg Talin phosphorylation sites mapped by mass spectrometry J. Cell Sci., November 1, 2005; 118(21): 4921 - 4923. [Full Text] [PDF]

				S. Komiya, M. Shimizu, J. Ikenouchi, S. Yonemura, T. Matsui, Y. Fukunaga, H. Liu, F. Endo, S. Tsukita, and A. Nagafuchi Apical membrane and junctional complex formation during simple epithelial cell differentiation of F9 cells Genes Cells, November 1, 2005; 10(11): 1065 - 1080. [Abstract] [Full Text] [PDF]

				A. V. Cybulsky, T. Takano, J. Papillon, K. Bijian, and J. Guillemette Activation of the extracellular signal-regulated kinase by complement C5b-9 Am J Physiol Renal Physiol, September 1, 2005; 289(3): F593 - F603. [Abstract] [Full Text] [PDF]

				G. Tamma, E. Klussmann, J. Oehlke, E. Krause, W. Rosenthal, M. Svelto, and G. Valenti Actin remodeling requires ERM function to facilitate AQP2 apical targeting J. Cell Sci., August 15, 2005; 118(16): 3623 - 3630. [Abstract] [Full Text] [PDF]

				A. Tamura, S. Kikuchi, M. Hata, T. Katsuno, T. Matsui, H. Hayashi, Y. Suzuki, T. Noda, S. Tsukita, and S. Tsukita Achlorhydria by ezrin knockdown: defects in the formation/expansion of apical canaliculi in gastric parietal cells J. Cell Biol., April 11, 2005; 169(1): 21 - 28. [Abstract] [Full Text] [PDF]

				P. Zhang, X. Tian, P. Chandra, and K. L. R. Brouwer Role of Glycosylation in Trafficking of Mrp2 in Sandwich-Cultured Rat Hepatocytes Mol. Pharmacol., April 1, 2005; 67(4): 1334 - 1341. [Abstract] [Full Text] [PDF]

				L. Heiska and O. Carpen Src Phosphorylates Ezrin at Tyrosine 477 and Induces a Phosphospecific Association between Ezrin and a Kelch-Repeat Protein Family Member J. Biol. Chem., March 18, 2005; 280(11): 10244 - 10252. [Abstract] [Full Text] [PDF]

				J. Ma, J. Zhou, S. Fan, L. Wang, X. Li, Q. Yan, M. Zhou, H. Liu, Q. Zhang, H. Zhou, et al. Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells Carcinogenesis, February 1, 2005; 26(2): 281 - 291. [Abstract] [Full Text] [PDF]

				T. Yokoyama, H. Goto, I. Izawa, H. Mizutani, and M. Inagaki Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis: possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM) Genes Cells, February 1, 2005; 10(2): 127 - 137. [Abstract] [Full Text] [PDF]

				J.-H. Lee, T. Katakai, T. Hara, H. Gonda, M. Sugai, and A. Shimizu Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation J. Cell Biol., October 25, 2004; 167(2): 327 - 337. [Abstract] [Full Text] [PDF]

				R. Nijhara, P. B. van Hennik, M. L Gignac, M. J. Kruhlak, P. L. Hordijk, J. Delon, and S. Shaw Rac1 Mediates Collapse of Microvilli on Chemokine-Activated T Lymphocytes J. Immunol., October 15, 2004; 173(8): 4985 - 4993. [Abstract] [Full Text] [PDF]

				S.-i. Kitajiri, K. Fukumoto, M. Hata, H. Sasaki, T. Katsuno, T. Nakagawa, J. Ito, S. Tsukita, and S. Tsukita Radixin deficiency causes deafness associated with progressive degeneration of cochlear stereocilia J. Cell Biol., August 16, 2004; 166(4): 559 - 570. [Abstract] [Full Text] [PDF]

				A. Ivetic, O. Florey, J. Deka, D. O. Haskard, A. Ager, and A. J. Ridley Mutagenesis of the Ezrin-Radixin-Moesin Binding Domain of L-selectin Tail Affects Shedding, Microvillar Positioning, and Leukocyte Tethering J. Biol. Chem., August 6, 2004; 279(32): 33263 - 33272. [Abstract] [Full Text] [PDF]

				H. Takenouchi, N. Kiyokawa, T. Taguchi, J. Matsui, Y. U. Katagiri, H. Okita, K. Okuda, and J. Fujimoto Shiga toxin binding to globotriaosyl ceramide induces intracellular signals that mediate cytoskeleton remodeling in human renal carcinoma-derived cells J. Cell Sci., August 1, 2004; 117(17): 3911 - 3922. [Abstract] [Full Text] [PDF]

				H. Zhao, H. Shiue, S. Palkon, Y. Wang, P. Cullinan, J. K. Burkhardt, M. W. Musch, E. B. Chang, and J. R. Turner Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport PNAS, June 22, 2004; 101(25): 9485 - 9490. [Abstract] [Full Text] [PDF]

				C. M. Finnerty, D. Chambers, J. Ingraffea, H. R. Faber, P. A. Karplus, and A. Bretscher The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain J. Cell Sci., March 15, 2004; 117(8): 1547 - 1552. [Abstract] [Full Text] [PDF]

				H. Matsumoto, T. Daikoku, H. Wang, E. Sato, and S.K. Dey Differential Expression of Ezrin/Radixin/Moesin (ERM) and ERM-Associated Adhesion Molecules in the Blastocyst and Uterus Suggests Their Functions During Implantation Biol Reprod, March 1, 2004; 70(3): 729 - 736. [Abstract] [Full Text] [PDF]

				A. V. Cybulsky, T. Takano, J. Papillon, A. Khadir, K. Bijian, and L. Le Berre The actin cytoskeleton facilitates complement-mediated activation of cytosolic phospholipase A2 Am J Physiol Renal Physiol, March 1, 2004; 286(3): F466 - F476. [Abstract] [Full Text]

				F. Pataky, R. Pironkova, and A. J. Hudspeth Radixin is a constituent of stereocilia in hair cells PNAS, February 24, 2004; 101(8): 2601 - 2606. [Abstract] [Full Text] [PDF]

				H. Kobayashi, J. Sagara, H. Kurita, M. Morifuji, M. Ohishi, K. Kurashina, and S.'i. Taniguchi Clinical Significance of Cellular Distribution of Moesin in Patients with Oral Squamous Cell Carcinoma Clin. Cancer Res., January 15, 2004; 10(2): 572 - 580. [Abstract] [Full Text] [PDF]

				M. J. Brown, R. Nijhara, J. A. Hallam, M. Gignac, K. M. Yamada, S. L. Erlandsen, J. Delon, M. Kruhlak, and S. Shaw Chemokine stimulation of human peripheral blood T lymphocytes induces rapid dephosphorylation of ERM proteins, which facilitates loss of microvilli and polarization Blood, December 1, 2003; 102(12): 3890 - 3899. [Abstract] [Full Text] [PDF]

				B. C. Bornhauser, P.-A. Olsson, and D. Lindholm MSAP Is a Novel MIR-interacting Protein That Enhances Neurite Outgrowth and Increases Myosin Regulatory Light Chain J. Biol. Chem., September 12, 2003; 278(37): 35412 - 35420. [Abstract] [Full Text] [PDF]

				Y. Miyamoto, J. Yamauchi, and H. Itoh Src Kinase Regulates the Activation of a Novel FGD-1-related Cdc42 Guanine Nucleotide Exchange Factor in the Signaling Pathway from the Endothelin A Receptor to JNK J. Biol. Chem., August 8, 2003; 278(32): 29890 - 29900. [Abstract] [Full Text] [PDF]

				D. A. Potter, A. Srirangam, K. A. Fiacco, D. Brocks, J. Hawes, C. Herndon, M. Maki, D. Acheson, and I. M. Herman Calpain Regulates Enterocyte Brush Border Actin Assembly and Pathogenic Escherichia coli-mediated Effacement J. Biol. Chem., August 8, 2003; 278(32): 30403 - 30412. [Abstract] [Full Text] [PDF]

				M. A. Chellaiah, R. S. Biswas, S. R. Rittling, D. T. Denhardt, and K. A. Hruska Rho-dependent Rho Kinase Activation Increases CD44 Surface Expression and Bone Resorption in Osteoclasts J. Biol. Chem., August 1, 2003; 278(31): 29086 - 29097. [Abstract] [Full Text] [PDF]

				E. A. McRobert, M. Gallicchio, G. Jerums, M. E. Cooper, and L. A. Bach The Amino-terminal Domains of the Ezrin, Radixin, and Moesin (ERM) Proteins Bind Advanced Glycation End Products, an Interaction That May Play a Role in the Development of Diabetic Complications J. Biol. Chem., July 3, 2003; 278(28): 25783 - 25789. [Abstract] [Full Text] [PDF]

				F. Jobard, B. Bouadjar, F. Caux, S. Hadj-Rabia, C. Has, F. Matsuda, J. Weissenbach, M. Lathrop, J.-F. Prud'homme, and J. Fischer Identification of mutations in a new gene encoding a FERM family protein with a pleckstrin homology domain in Kindler syndrome Hum. Mol. Genet., April 15, 2003; 12(8): 925 - 935. [Abstract] [Full Text] [PDF]

				F. E. McCann, B. Vanherberghen, K. Eleme, L. M. Carlin, R. J. Newsam, D. Goulding, and D. M. Davis The Size of the Synaptic Cleft and Distinct Distributions of Filamentous Actin, Ezrin, CD43, and CD45 at Activating and Inhibitory Human NK Cell Immune Synapses J. Immunol., March 15, 2003; 170(6): 2862 - 2870. [Abstract] [Full Text] [PDF]

				W. J. Smith, N. Nassar, A. Bretscher, R. A. Cerione, and P. A. Karplus Structure of the Active N-terminal Domain of Ezrin. CONFORMATIONAL AND MOBILITY CHANGES IDENTIFY KEYSTONE INTERACTIONS J. Biol. Chem., February 7, 2003; 278(7): 4949 - 4956. [Abstract] [Full Text] [PDF]

				Y. Samstag, S. M. Eibert, M. Klemke, and G. H. Wabnitz Actin cytoskeletal dynamics in T lymphocyte activation and migration J. Leukoc. Biol., January 1, 2003; 73(1): 30 - 48. [Abstract] [Full Text] [PDF]

				V. Orian-Rousseau, L. Chen, J. P. Sleeman, P. Herrlich, and H. Ponta CD44 is required for two consecutive steps in HGF/c-Met signaling Genes & Dev., December 1, 2002; 16(23): 3074 - 3086. [Abstract] [Full Text] [PDF]

				T. Kubo, T. Yamashita, A. Yamaguchi, H. Sumimoto, K. Hosokawa, and M. Tohyama A Novel FERM Domain Including Guanine Nucleotide Exchange Factor Is Involved in Rac Signaling and Regulates Neurite Remodeling J. Neurosci., October 1, 2002; 22(19): 8504 - 8513. [Abstract] [Full Text] [PDF]

				E. Medina, J. Williams, E. Klipfell, D. Zarnescu, G. Thomas, and A. Le Bivic Crumbs interacts with moesin and {beta}Heavy-spectrin in the apical membrane skeleton of Drosophila J. Cell Biol., September 3, 2002; 158(5): 941 - 951. [Abstract] [Full Text] [PDF]

				Y. Sako, M. Nakao, K. Nakaya, H. Yamasaki, B. Gottstein, M. W. Lightowers, P. M. Schantz, and A. Ito Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18 J. Clin. Microbiol., August 1, 2002; 40(8): 2760 - 2765. [Abstract] [Full Text] [PDF]

				K. L. Mitchem, E. Hibbard, L. A. Beyer, K. Bosom, G. A. Dootz, D. F. Dolan, K. R. Johnson, Y. Raphael, and D. C. Kohrman Mutation of the novel gene Tmie results in sensory cell defects in the inner ear of spinner, a mouse model of human hearing loss DFNB6 Hum. Mol. Genet., August 1, 2002; 11(16): 1887 - 1898. [Abstract] [Full Text] [PDF]

				S. Yonemura, T. Matsui, S. Tsukita, and S. Tsukita Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo J. Cell Sci., June 15, 2002; 115(12): 2569 - 2580. [Abstract] [Full Text] [PDF]

				E. G. Ponimaskin, J. Profirovic, R. Vaiskunaite, D. W. Richter, and T. A. Voyno-Yasenetskaya 5-Hydroxytryptamine 4(a) Receptor Is Coupled to the Galpha Subunit of Heterotrimeric G13 Protein J. Biol. Chem., May 31, 2002; 277(23): 20812 - 20819. [Abstract] [Full Text] [PDF]

				S. Benvenuti, R. Cramer, C. C. Quinn, J. Bruce, M. Zvelebil, S. Corless, J. Bond, A. Yang, S. Hockfield, A. L. Burlingame, et al. Differential Proteome Analysis of Replicative Senescence in Rat Embryo Fibroblasts Mol. Cell. Proteomics, April 1, 2002; 1(4): 280 - 292. [Abstract] [Full Text] [PDF]

				T. Shimizu, A. Seto, N. Maita, K. Hamada, S. Tsukita, S. Tsukita, and T. Hakoshima Structural Basis for Neurofibromatosis Type 2. CRYSTAL STRUCTURE OF THE MERLIN FERM DOMAIN J. Biol. Chem., March 15, 2002; 277(12): 10332 - 10336. [Abstract] [Full Text] [PDF]

				J. M. Serrador, M. Vicente-Manzanares, J. Calvo, O. Barreiro, M. C. Montoya, R. Schwartz-Albiez, H. Furthmayr, F. Lozano, and F. Sanchez-Madrid A Novel Serine-rich Motif in the Intercellular Adhesion Molecule 3 Is Critical for Its Ezrin/Radixin/Moesin-directed Subcellular Targeting J. Biol. Chem., March 15, 2002; 277(12): 10400 - 10409. [Abstract] [Full Text] [PDF]

				K. Itoh, M. Sakakibara, S. Yamasaki, A. Takeuchi, H. Arase, M. Miyazaki, N. Nakajima, M. Okada, and T. Saito Cutting Edge: Negative Regulation of Immune Synapse Formation by Anchoring Lipid Raft to Cytoskeleton Through Cbp-EBP50-ERM Assembly J. Immunol., January 15, 2002; 168(2): 541 - 544. [Abstract] [Full Text] [PDF]

				A. Algeciras-Schimnich, L. Shen, B. C. Barnhart, A. E. Murmann, J. K. Burkhardt, and M. E. Peter Molecular Ordering of the Initial Signaling Events of CD95 Mol. Cell. Biol., January 1, 2002; 22(1): 207 - 220. [Abstract] [Full Text] [PDF]

				K. Lange Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism Am J Physiol Cell Physiol, January 1, 2002; 282(1): C1 - C26. [Abstract] [Full Text] [PDF]

				V. L. Bonilha and E. Rodriguez-Boulan Polarity and Developmental Regulation of Two PDZ Proteins in the Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3274 - 3282. [Abstract] [Full Text] [PDF]

				R. Vaiskunaite, T. Kozasa, and T. A. Voyno-Yasenetskaya Interaction between the Galpha Subunit of Heterotrimeric G12 Protein and Hsp90 Is Required for Galpha 12 Signaling J. Biol. Chem., November 30, 2001; 276(49): 46088 - 46093. [Abstract] [Full Text] [PDF]

				T. Lockwich, B. B. Singh, X. Liu, and I. S. Ambudkar Stabilization of Cortical Actin Induces Internalization of Transient Receptor Potential 3 (Trp3)-associated Caveolar Ca2+ Signaling Complex and Loss of Ca2+ Influx without Disruption of Trp3-Inositol Trisphosphate Receptor Association J. Biol. Chem., November 2, 2001; 276(45): 42401 - 42408. [Abstract] [Full Text] [PDF]

				O.-M. Mykkanen, M. Gronholm, M. Ronty, M. Lalowski, P. Salmikangas, H. Suila, and O. Carpen Characterization of Human Palladin, a Microfilament-associated Protein Mol. Biol. Cell, October 1, 2001; 12(10): 3060 - 3073. [Abstract] [Full Text] [PDF]

				J. A. Weiner, N. Fukushima, J. J. A. Contos, S. S. Scherer, and J. Chun Regulation of Schwann Cell Morphology and Adhesion by Receptor-Mediated Lysophosphatidic Acid Signaling J. Neurosci., September 15, 2001; 21(18): 7069 - 7078. [Abstract] [Full Text] [PDF]

				R. E. Ward IV, L. Schweizer, R. S. Lamb, and R. G. Fehon The Protein 4.1, Ezrin, Radixin, Moesin (FERM) Domain of Drosophila Coracle, a Cytoplasmic Component of the Septate Junction, Provides Functions Essential for Embryonic Development and Imaginal Cell Proliferation Genetics, September 1, 2001; 159(1): 219 - 228. [Abstract] [Full Text] [PDF]

				R. A. ORLANDO, T. TAKEDA, B. ZAK, S. SCHMIEDER, V. M. BENOIT, T. MCQUISTAN, H. FURTHMAYR, and M. G. FARQUHAR The Glomerular Epithelial Cell Anti-Adhesin Podocalyxin Associates with the Actin Cytoskeleton through Interactions with Ezrin J. Am. Soc. Nephrol., August 1, 2001; 12(8): 1589 - 1598. [Abstract] [Full Text] [PDF]

				K. J. Bame Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans Glycobiology, June 1, 2001; 11(6): 91R - 98R. [Abstract] [Full Text] [PDF]

				C. V. Melendez-Vasquez, J. C. Rios, G. Zanazzi, S. Lambert, A. Bretscher, and J. L. Salzer Nodes of Ranvier form in association with ezrin-radixin-moesin (ERM)-positive Schwann cell processes PNAS, January 30, 2001; 98(3): 1235 - 1240. [Abstract] [Full Text] [PDF]

				C.-G. Koh, E. Manser, Z.-S. Zhao, C.-P. Ng, and L. Lim {beta}1PIX, the PAK-interacting exchange factor, requires localization via a coiled-coil region to promote microvillus-like structures and membrane ruffles J. Cell Sci., January 12, 2001; 114(23): 4239 - 4251. [Abstract] [Full Text] [PDF]

				C. E. Pierreux, F. J. Nicolás, and C. S. Hill Transforming Growth Factor beta -Independent Shuttling of Smad4 between the Cytoplasm and Nucleus Mol. Cell. Biol., December 1, 2000; 20(23): 9041 - 9054. [Abstract] [Full Text]

				D G. R Evans, M Sainio, and M. E Baser Neurofibromatosis type 2 J. Med. Genet., December 1, 2000; 37(12): 897 - 904. [Abstract] [Full Text]

				E. J. Weinman, C. Minkoff, and S. Shenolikar Signal complex regulation of renal transport proteins: NHERF and regulation of NHE3 by PKA Am J Physiol Renal Physiol, September 1, 2000; 279(3): F393 - F399. [Abstract] [Full Text] [PDF]

				M. D. Schaller UNC112: A New Regulator of Cell-Extracellular Matrix Adhesions? J. Cell Biol., July 11, 2000; 150(1): F9 - F12. [Abstract] [Full Text] [PDF]

				J. Berg, B. Derfler, C. Pennisi, D. Corey, and R. Cheney Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin J. Cell Sci., January 10, 2000; 113(19): 3439 - 3451. [Abstract] [PDF]

				C Wadham, J. Gamble, M. Vadas, and Y Khew-Goodall Translocation of protein tyrosine phosphatase Pez/PTPD2/PTP36 to the nucleus is associated with induction of cell proliferation J. Cell Sci., January 9, 2000; 113(17): 3117 - 3123. [Abstract] [PDF]

				H. Q. Sun, M. Yamamoto, M. Mejillano, and H. L. Yin Gelsolin, a Multifunctional Actin Regulatory Protein J. Biol. Chem., November 19, 1999; 274(47): 33179 - 33182. [Full Text] [PDF]

				S. L. Nix, A. H. Chishti, J. M. Anderson, and Z. Walther hCASK and hDlg Associate in Epithelia, and Their Src Homology 3 and Guanylate Kinase Domains Participate in Both Intramolecular and Intermolecular Interactions J. Biol. Chem., December 22, 2000; 275(52): 41192 - 41200. [Abstract] [Full Text] [PDF]

				P. Kussel-Andermann, A. El-Amraoui, S. Safieddine, J.-P. Hardelin, S. Nouaille, J. Camonis, and C. Petit Unconventional Myosin VIIA Is a Novel A-kinase-anchoring Protein J. Biol. Chem., September 15, 2000; 275(38): 29654 - 29659. [Abstract] [Full Text] [PDF]

				R. Nguyen, D. Reczek, and A. Bretscher Hierarchy of Merlin and Ezrin N- and C-terminal Domain Interactions in Homo- and Heterotypic Associations and Their Relationship to Binding of Scaffolding Proteins EBP50 and E3KARP J. Biol. Chem., March 2, 2001; 276(10): 7621 - 7629. [Abstract] [Full Text] [PDF]

				S. M. Gisler, I. Stagljar, M. Traebert, D. Bacic, J. Biber, and H. Murer Interaction of the Type IIa Na/Pi Cotransporter with PDZ Proteins J. Biol. Chem., March 16, 2001; 276(12): 9206 - 9213. [Abstract] [Full Text] [PDF]

				C. G. Broustas, N. Grammatikakis, M. Eto, P. Dent, D. L. Brautigan, and U. Kasid Phosphorylation of the Myosin-binding Subunit of Myosin Phosphatase by Raf-1 and Inhibition of Phosphatase Activity J. Biol. Chem., January 18, 2002; 277(4): 3053 - 3059. [Abstract] [Full Text] [PDF]

				A. Ivetic, J. Deka, A. Ridley, and A. Ager The Cytoplasmic Tail of L-selectin Interacts with Members of the Ezrin-Radixin-Moesin (ERM) Family of Proteins. CELL ACTIVATION-DEPENDENT BINDING OF MOESIN BUT NOT EZRIN J. Biol. Chem., January 11, 2002; 277(3): 2321 - 2329. [Abstract] [Full Text] [PDF]

				M. Diakonova, G. Bokoch, and J. A. Swanson Dynamics of Cytoskeletal Proteins during Fcgamma Receptor-mediated Phagocytosis in Macrophages Mol. Biol. Cell, February 1, 2002; 13(2): 402 - 411. [Abstract] [Full Text] [PDF]

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