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J Biol Chem, Vol. 274, Issue 53, 37908-37914, December 31, 1999

A Role for Polyproline Motifs in the Spinal Muscular Atrophy Protein SMN
PROFILINS BIND TO AND COLOCALIZE WITH SMN IN NUCLEAR GEMS^*

Torsten Giesemann, Silvia Rathke-Hartlieb^§, Martin Rothkegel, Jörg W. Bartsch^§, Sabine Buchmeier, Brigitte M. Jockusch, and Harald Jockusch^§¶

From the  Department of Cell Biology, Zoological Institute, Technical University of Braunschweig, D-38092 Braunschweig, Germany, and the ^§ Department of Developmental Biology and Molecular Pathology, W7, University of Bielefeld, D-33501 Bielefeld, Germany

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by the loss of -motoneurons in the spinal cord followed by atrophy of skeletal muscles. SMA-determining candidate genes, SMN1 and SMN2, have been identified on human chromosome 5q. The corresponding SMN protein is expressed ubiquitously. It is coded by seven exons and contains conspicuous proline-rich motifs in its COOH-terminal third (exons 4, 5, and 6). Such motifs are known to bind to profilins (PFNs), small proteins engaged in the control of actin dynamics. We tested whether profilins interact with SMN via its polyproline stretches. Using the yeast two-hybrid system we show that profilins bind to SMN and that this binding depends on its proline-rich motifs. These results were confirmed by coimmunoprecipitation and by in vitro binding studies. Two PFN isoforms, I and II, are known, of which II is characteristic for central nervous system tissue. We show by in situ hybridization that both PFNs are highly expressed in mouse spinal cord and that PFN II is expressed predominantly in neurons. In motoneurons, the primary target of neurodegeneration in SMA, profilins are highly concentrated and colocalize with SMN in the cytoplasm of the cell body and in nuclear gems. Likewise, SMN and PFN I colocalize in gems of HeLa cells. Although SMN interacts with both profilin isoforms, binding of PFN II was stronger than of PFN I in all assays employed. Because the SMN genes are expressed ubiquitously, our findings suggest that the interaction of PFN II with SMN may be involved in neuron-specific effects of SMN mutations.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Spinal muscular atrophies (SMAs)¹ types I, II, and III are autosomal hereditary diseases of graded severity in which loss of motoneurons leads to paralysis and subsequent atrophy of skeletal muscles, and in the most severe Werdnig-Hoffmann type I form, to death in early infancy. The corresponding SMA disease genes have been mapped to human chromosome 5q (1). There are two genes in close vicinity, the telomeric SMN1 (or SMN^T) and the centromeric SMN2 (or SMN^C; 2). Although they have identical coding sequences for a 294-amino acid SMN polypeptide, pathogenic mutations were found solely in SMN1. Its gene product, the 40-kDa protein "survival motoneuron," SMN, is expressed ubiquitously, and its concentration is reduced drastically in the spinal cord of SMA patients (3, 4). There is evidence, mostly from a yeast two-hybrid screen and from a Xenopus oocyte model system, that the SMN protein is engaged in the assembly of spliceosomal U snRNPs in the cytoplasm (5-7). Recently, the function of SMN has been demonstrated by a dominant-negative mutant of SMN which inhibits pre-mRNA splicing by blocking the formation of a mature spliceosome (8).

The severity of SMA has been correlated with a deficient oligomerization of mutated SMN proteins, and it has been hypothesized that the critical level of functional SMN oligomers in normal motoneurons may be controlled by SMN's binding to a motoneuron-specific factor (9). There are seven coding exons in the human SMN gene, and SMN splice variants lacking exons 5, 7, or both are found. SMN2 expresses predominantly a 7 truncated variant that is probably not capable of exerting all biological functions of SMN. This explains why the telomeric SMN1 gene is indispensable for normal survival of motoneurons in humans (4). Pathogenic mutations involve deletions, frameshift and missense mutations in exons 5, 6, and 7 of SMN1 as well as the conversion of SMN1 to SMN2 which drastically reduces exon 7 expression (2, 10-14).

In the mouse, there is only one gene coding for SMN, located on chromosome 13, within the region of conserved synteny with human chromosome 5q13 (15). No alternative splicing of the transcripts has been found in the mouse. The predicted amino acid sequence of mouse SMN is 82% identical to that of the human protein, including a conserved putative nuclear localization signal (15). No murine mutations homologous to human chromosome 5q SMAs have been found (16), and a functional knockout of the gene coding for SMN causes death of early embryos (17) suggesting, in accordance with biochemical evidence (6), that SMN is an essential housekeeping protein (18).

Several ligand binding sites have been identified in SMN protein. Based on a yeast two-hybrid screen using SMN as a bait, SIP-1 (SMN-interacting protein 1) was selected and shown to interact with SMN in vivo and in vitro (6). The SMN·SIP-1 complexes are large (approximately 300 kDa) and contain additional proteins several of which were shown to be U snRNP proteins (6). The proteins within this complex are involved in spliceosomal snRNP biogenesis (7) and in pre-mRNA splicing (8). Two of these proteins contain an Sm domain (recognized by autoimmune sera of the Sm serotype), SmB and SmB', and bind recombinant SMN in vitro (6). These results correlate with the localization of SMN protein in nuclear structures called gems (5) and, assuming that SMN and SIP-1 are involved in the recycling of U snRNP in the nucleus, also in the cytoplasm (3, 5).

The polyproline stretch in the SMN protein contains a symmetric motif with the sequence Pro₅-X₁₇-Pro₁₀-X₁₇-Pro₅ (with X specifying any amino acid residue); no similar motif has been described for any other protein. This motif is highly conserved between human and mouse SMN (2, 15), implying that it has an essential function. Several polypeptides comprising proline clusters regulate actin polymerization (19). As polyproline-rich ligands of profilins, the mammalian proteins VASP (20), Mena (21), and p140mDia (22) have been characterized. In several mammals, two isoforms of profilins have been identified (see references in 23) which differ slightly in their affinities for the various ligands. In particular, bovine profilin I (PFN I) was found to have a lower affinity for polyproline motifs than profilin II (PFN II; 24). PFN I is expressed early in mammalian embryogenesis (25) and is distributed widely in various cells and tissues, whereas PFN II is not expressed in most tissues tested but is highly concentrated in the central nervous system and in cultured neurons (26).

Prompted by the conspicuous proline stretches in SMN, we analyzed the possible interaction of this protein with both isoforms of profilin, PFN I and PFN II.

A preliminary report of this work has been given (27).

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Yeast Two-hybrid Assay-- cDNA fragments encoding human SMN, SMN7 (gift of Dr. Judith Melki, Illkirch), and mouse profilins I and II (gift of Dr. Walter Witke, Monterotondo, Italy) were subcloned into the yeast expression plasmids pGBT9 in-frame to the GAL4 DNA binding domain and into pGAD424 in-frame to the GAL4 activating domain, respectively. Deletion fragments SMN-N (encoding amino acids 1-188), SMN-C (encoding 188-294), and SMN7-C (encoding 188-278) were inserted into the vectors pGBT9 and pGAD424 using synthetic oligodeoxynucleotides as linkers. For all constructs, the correct reading frames were confirmed by sequencing. The preparation of all media and reagents and all manipulations of the yeast strains HF7c and SFY as well as the -galactosidase filter assay were performed according to the Matchmaker protocol (CLONTECH, Heidelberg).

In Vitro Transcription/Translation and Production of Fusion Proteins-- The coding regions of human SMN and SMN7 and mouse PFN II were cloned into the vector pcDNA3 (Invitrogen, Groningen). The [³⁵S]methionine-labeled proteins were synthesized by in vitro transcription/translation using the TNT-coupled reticulocyte lysate system (Promega, Heidelberg) according to the manufacturer's protocol. The maltose-binding protein-SMN fusion protein (MBP-SMN) was expressed in the pMal-c2 bacterial expression system (New England Biolabs, Schwalbach/Ts, FRG) in Escherichia coli strain DH5' and purified using amylose affinity chromatography according to the manufacturer's manual. The elution fractions were analyzed by SDS-PAGE and immunoblot (a-MBP antibody, New England Biolabs) and concentrated using Ultrafree-4 centrifugation tubes (Millipore, Eschborn, FRG). For the expression of recombinant mouse PFN II the T7 RNA polymerase expression system (28) was used. The E. coli strain BL21(DE3) pLysS was transformed with the expression plasmid pMW172/PFN II, containing the coding region of mouse PFN II. The expressed protein was purified by poly-(L-proline) affinity chromatography as described previously (29). Eluted fractions were pooled and dialyzed against 10 mM Tris/HCl, pH 7.6, 2 mM CaCl₂, 1.25 mM dithiothreitol. The purity of the protein preparations was better than 95%, as judged by Coomassie Blue-stained SDS-gel profiles.

Dot Overlay Assays-- 100 pmol of MBP (New England Biolabs) and recombinant MBP-SMN fusion protein or 500 pmol of mouse PFN I and II were immobilized on a nitrocellulose membrane using a dot blotter with circular slots of 5-mm diameter (Biometra, Göttingen). The transfer was checked by reversible Ponceau staining, and unspecific binding sites were blocked with 5% non-fat milk powder in phosphate-buffered saline (PBS; 140 mM NaCl, 10 mM Na,K phosphate, pH 7.3). In vitro translated proteins were diluted in PBS containing 1% (w/v) BSA and 20 mM -mercaptoethanol. The membrane was incubated in this solution at 4 °C overnight. After intensive washing with PBS containing 0.1% (v/v) Triton X-100, bound proteins were detected by autoradiography.

Affinity Precipitation-- Purified recombinant mouse PFN II and BSA (as a negative control) were coupled to NHS-Sepharose 4B Fast Flow (Amersham Pharmacia Biotech, Uppsala) with an efficiency of 10-15 µg of bound protein/µl of NHS material. Free NHS groups were blocked with ethanolamine. Aliquots of 25 µl in vitro transcription/translation reaction mixture were diluted in PBS and incubated with 10 µl of conjugated Sepharose for 4 h at 4 °C. After sedimentation, the beads were washed successively with standard PBS, 140 mM NaCl, high salt saline, 290 mM NaCl, and PBS with 0.2% Triton X-100. The SDS-denatured proteins of the first supernatant and of the washed pellets were separated by SDS-PAGE, and the gel was blotted onto nitrocellulose membrane. The ³⁵S-labeled polypeptides were detected by autoradiography.

Tissues and Cells-- Spinal cords were dissected from freshly killed 40-60-day-old mice (strain C57BL/6). Immunopositive gems were only observed when the preparations were done rather swiftly until the tissue blocks were shock frozen in liquid nitrogen-isopropane. In some cases mice were perfused under deep anesthesia with a solution of 9 g/liter NaCl, 25 g/liter polyvinylpyrrolidone (40 kDa), 0.25 g/liter heparin, and 5 g/liter procaine-HCl, pH 7.2, to remove blood plasma. Perfusions were done in accordance with the German law for the protection of animals with a permit by the local authority.

HeLa cells were cultured either directly in 10-cm dishes or on glass coverslips coated with collagen in Dulbecco's modified Eagle's medium (Biochrom KG, Berlin) supplemented with 10% fetal calf serum (PAA Laboratories, Cölbe, FRG), according to standard procedures.

In Situ Hybridization-- Riboprobes specific for PFN I and II were prepared from cDNAs containing the entire coding region (PFN I, accession number X14425; PFN II,²), cloned in pGEM-3 (Promega). The riboprobes were generated by in vitro transcription with SP6 (sense for PFN I, antisense for PFN II) and T7 (antisense for PFN I, sense for PFN II) polymerase with fluorescein-conjugated UTP (in situ color kit, Amersham Pharmacia Biotech). Spinal cord segments were fixed in 3% paraformaldehyde in buffered saline and embedded in paraffin according to standard procedures. Deparaffinized sections (5 µm) were hybridized with riboprobes (400 ng/ml) overnight at 55 °C and subsequently washed with 1 × SSC, 0.1% SDS, 0.2 × SSC, 0.1% SDS at 55 °C for 10 min followed by 0.1 × SSC, 0.1% SDS at 55 °C for 5 min. After washing, samples were treated with RNase A (10 µg/ml in 2 × SSC) for 15 min at 37 °C. Bound riboprobe was detected with an anti-fluorescein antibody coupled to alkaline phosphatase and subsequent color reaction using nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate as substrate.

Antibodies-- 12 anti-profilin hybridoma clones (with recombinant mouse PFN I and II as immunogens) have been used in the course of this study. Several monoclonal antibodies showed isoform specificity in immunoblots with SDS-denatured antigens but not on sections. Some antibodies recognized diffuse PFN but not PFN in gems. A possible explanation for these differences might be epitope masking or modification. Several antibodies recognized human and bovine PFN in immunoblots and cultured cells but not mouse PFNs. Problems with mouse monoclonal antibodies on mouse tissue sections, due to endogenous mouse IgG, could in part be overcome by perfusion of the animals. Results obtained with the following antibodies were included in this study: monoclonal antibody 2B1 directed against human SMN (IgG; generous gift of Dr. G. Dreyfuss, Ref. 5, and purchased from Transduction Labs, Lexington, KY), henceforth designated mc-a-SMN; a commercial polyclonal antibody that recognizes both PFN isoforms (Alexis Co., San Diego, CA), here designated pc-anti-PFN; monoclonal IgG 2H11, raised against a fusion protein of an -actinin domain with bovine PFN I, which reacts specifically with PFN I of many mammalian species, but not with rodents (30), designated mc-a-PFN I; and a monoclonal IgG raised against recombinant mouse PFN II, 5C6, here designated mc-a-PFN II. For pc-a-PFN (Alexis) the specificity was verified on Western blots of extracts from cultured cells and tissues including mouse spinal cord; only a ~15-kDa band was stained. Monoclonal antibody 2H11 has been described extensively (30). mc-a-PFN II was tested for its isoform specificity by enzyme-linked immunosorbent assay and Western blotting on extracts of E. coli expressing recombinant PFN I and PFN II, respectively, and stained a band of ~15 kDa band only in the presence of PFN II.

Immunoprecipitation of Protein Complexes after in Situ Cross-linking-- In situ cross-linking, precipitation, and analysis of protein complexes were performed as described (31, 32) with slight modifications. HeLa cells were grown on 10-cm dishes (2.5 × 10⁶/dish), rinsed in PBS, and incubated at room temperature for 30 min with a 0.5 mM concentration of the membrane-permeant cross-linker dithiobis(succinimidyl propionate) (Pierce, Sankt Augustin, Germany). Excess cross-linker was quenched with 0.2 M glycine. After additional rinsing in PBS, cells were lysed in RIPA buffer (50 mM Tris, pH 7.2, 1% (v/v) Triton X-100, 0.25% desoxycholate, 1 mM EGTA, 150 mM NaCl, 20 mM glycine, 2.5 mM sodium azide, 1 µM pepstatin A, 80 µM Pefabloc SC, 0.46 µM aprotinin; 30 min at 4 °C). Cellular material was then scraped off the dish with a rubber policeman, homogenized by pipetting, and centrifuged at 15,000 × g at 4 °C for 10 min. 2 µl of mc-a-SMN was added to the supernatant, and the samples were incubated for 16 h at 4 °C. Then 50 µl of a 50% slurry of protein G-Sepharose preblocked with 2% (w/v) BSA in RIPA buffer was added to the samples, which were then incubated further under stirring at 4 °C for 1 h. The Sepharose beads were collected by centrifugation and washed twice in RIPA buffer and once with PBS before the samples were boiled in SDS sample buffer containing 20% (v/v) -mercaptoethanol, to cleave the cross-linker. Finally, samples were analyzed by SDS-PAGE and immunoblotting, using standard procedures. For control precipitations, we used the monoclonal antibody 4A6, which does not react with any vertebrate protein but specifically recognizes an epitope present exclusively in profilins of birch-related plants (33, 34).

Immunohistochemistry-- For immunofluorescence, cryosections and cells were fixed with methanol at 20 °C for 6 min. Monoclonal antibodies were used as undiluted tissue culture supernatants. Goat anti-mouse IgG, conjugated with Cy3 (red, Dianova, Hamburg) served as a second antibody. For double staining, Fab fragments of Cy2-conjugated (green) goat anti-mouse IgG (Dianova) were used to saturate the anti-SMN before incubation with mc-a-PFN II and Cy3-conjugated goat anti-mouse IgG. Fluorescent images were photographed on a Zeiss Axiophot fluorescence microscope. Color slides were scanned and images processed using Adobe Photoshop (version 5.0).

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

SMN and Profilins Interact in the Yeast Two-hybrid System-- To test for a direct interaction between SMN and PFN we employed the yeast two-hybrid system. Appropriate vectors comprising cDNA fragments encoding mouse PFN I and II and human SMN (Fig. 1A), SMN7, NH₂- and COOH-terminal deletion fragments of SMN, and a COOH-terminal deletion fragment of SMN7 were generated (Fig. 1B). SMN-N, encoding amino acids 1-188, and SMN-C, encoding amino acids 188-294, were obtained by cleavage at the NcoI site at base pair 565 within the coding region of the SMN gene. The COOH-terminal deletion fragment contained the proline-rich region with the P₅, P₁₀, P₅ motifs. The results of the yeast two-hybrid screens are shown in Table I. SMN protein interacted strongly with itself (5, 9), with PFN II, and less well with PFN I. SMN-SMN interactions were shown to be mediated by the COOH-terminal third of SMN. These findings correspond to the location of the recently determined oligomerization domain of SMN, a 30-amino acid region between residues 249 and 278 (9; Fig. 1A). The same fragment, which contains 54% proline residues between amino acids 190 and 294, was found to be responsible for PFN binding. SMN7 and SMN7-C did not interact with any of the partners (Table I).

View larger version (25K): [in this window] [in a new window]   Fig. 1.   Functional domains in the SMN polypeptide and constructs used for protein-protein interaction studies. Panel A, the full-length SMN protein is shown as a bar with exons separated by vertical lines and numbered above; black boxes symbolize proline-rich motifs. Boxes below refer to interaction sites with ligand proteins: SIP-1 (6); SmB, a protein of the U snRNP complex (6); PFN I and PFN II (this work); SMN, self-association (9). Panel B, SMN7 truncated form (natural splice isoform) and deletion fragments used in the yeast two-hybrid system.

Interaction of SMN and Profilin in Mammalian Cells-- To corroborate the results obtained in the yeast two-hybrid system we performed coimmunoprecipitations with HeLa cell extracts. Cells were treated with the membrane-permeant cross-linker dithiobis(succinimidyl propionate) before lysis, in order to stabilize protein complexes within the living cells (31, 32). The complexes were immunoprecipitated with mc-a-SMN and protein G-Sepharose and processed for SDS-PAGE and immunoblotting. As shown in Fig. 2, the precipitates contained both proteins, SMN and profilin, indicating that SMN and profilin form complexes under the competitive conditions in the cytosol of cells.

View larger version (54K): [in this window] [in a new window]   Fig. 2.   SMN·PFN complex formation in HeLa cells as shown by in situ cross-linking and subsequent coimmunoprecipitation. HeLa cells were treated with the membrane-permeant cross-linker dithiobis(succinimidyl propionate) (31, 32). SMN and bound proteins were immunoprecipitated (IP) with mc-a-SMN antibody; mc-a-birch pollen profilin, not reactive with mammalian profilins (33, 34), served as unspecific control. Precipitated proteins were detected by immunoblot (IB) with mc-a-PFN I or mc-a-SMN as indicated. LC, IgG, light chain.

SMN and Profilins Interact in Vitro-- To characterize further the interaction between SMN and profilin we performed solid phase binding assays on membranes. In vitro translated, radioactively labeled SMN and mouse profilins, a recombinant fusion protein (MBP-SMN) comprising SMN and the MBP of E. coli as a fusion partner, and recombinant mouse profilins were used in this assay. As shown in Fig. 3A, in vitro translated SMN bound to immobilized MBP-SMN but not to MBP alone. Recombinant PFN II bound strongly to immobilized MBP-SMN, whereas the interaction with mouse PFN I was rather weak. In the converse experiment, in vitro translated SMN also bound strongly to immobilized PFN II but weakly to PFN I. The significance of the SMN polyproline stretches for this interaction was tested in competition experiments. The addition of peptides consisting of 50-60 proline residues (poly-Pro) inhibited the complex formation between membrane-adsorbed MBP-SMN and in vitro translated PFN II. As seen in Fig. 3B, a 25-fold molar excess of poly-Pro over SMN virtually abolished the interaction.

View larger version (45K): [in this window] [in a new window]   Fig. 3.   Interaction of SMN with mouse PFN isoforms in vitro. Panels A and B, solid phase overlay assays. Panel A, comparison of SMN binding by PFN I and II. 100 pmol of MBP and MBP-SMN fusion protein or 500 pmol of recombinant mouse PFN I and mouse PFN II per spot were immobilized on a membrane and were incubated with 35S-labeled in vitro translated proteins in the overlay. Bound protein was detected by autoradiography. Panel B, effect of poly-L-proline on the interaction of PFN II with SMN. 100 pmol of MBP-SMN fusion protein per spot was immobilized and incubated with in vitro translated mouse PFN II in the presence of increasing concentrations of poly-L-proline (poly-Pro). Panel C, affinity precipitation of in vitro translated SMN and SMN7 with PFN II bound to beads and effects of poly-L-proline. Translation efficiency and purity of [35S]methionine-labeled proteins were checked by PAGE and autoradiography (Total). 120 µg of mouse PFN II was coupled on 10 µl of NHS-HiTrap material. The material was incubated with in vitro translated 35S[Met-labeled SMN and SMN7. BSA, negative control. Poly-Pro, a 5-fold excess of poly-L-proline over coupled mouse PFN II. Samples of the supernatants (S) and pellets (P) were separated by SDS-PAGE and blotted. 35S-labeled SMN was detected by autoradiography. Different positions on the gel are the result of interference with electrophoretic mobility by salt in supernatants.

Lastly, affinity precipitation with Sepharose-coupled proteins was performed. PFN II covalently coupled to Sepharose beads was incubated with in vitro translated [³⁵S]methionine-labeled SMN. Bound and free SMN were separated by centrifugation and detected by SDS-gel electrophoresis and autoradiography. As shown in Fig. 3C, Sepharose-coupled recombinant PFN II bound SMN, whereas control BSA-Sepharose did not. Again, SMN/PFN II binding was sensitive to the presence of poly-Pro: a 5-fold molar excess over SMN completely abolished the interaction. However, in contrast to the results obtained with the yeast two-hybrid system, in vitro translated SMN7 did bind to Sepharose-coupled PFN II.

The data from the yeast two-hybrid experiments and the in vitro data allow the conclusion that SMN binds PFN II stronger than PFN I. Because mouse PFN II is characteristic for central nervous system tissue (26) we examined the expression of the mRNAs for both profilin isoforms and SMN in the spinal cord.

PFNs I and II mRNAs Are Highly Expressed in the Spinal Cord-- To analyze the expression pattern of PFNs in the spinal cord, the organ affected in SMA, in situ hybridizations were performed using mouse PFN I- and PFN II-specific riboprobes (Fig. 4). PFN I mRNA was present in a variety of cell types, including motoneurons and interneurons of the gray as well as in astrocytes of the white matter, whereas PFN II mRNA expression was restricted to motoneurons in the anterior horn and some interneurons. Hence, motoneurons appeared especially rich in the mRNA of both PFN isoforms.

View larger version (177K): [in this window] [in a new window]   Fig. 4.   PFN I and II mRNA expression in mouse spinal cord sections. Panels A and B, cross-sections of adult mouse cervical spinal cord (dorsal, up; lateral, left) hybridized with antisense probes against PFN I (panel A) and PFN II (panel B), respectively. The border between gray (G in panel C) and white (W) matter is delineated by dashed lines, revealing that PFN I is expressed in a variety of cells in both areas, whereas PFN II expression is predominant in the neurons within the gray matter. Panel C, a corresponding section incubated with a PFN II sense probe (negative control). Panel D, enlarged part of the ventral horn of the section in panel B, showing hybridization of PFN II probe with a group of motoneurons. Panel E, parallel section stained with cresyl violet (Nissl) to show cell bodies of motoneurons. Bar in panel A, 100 µm for A, B, and C. Bar in panel D, 50 µm for D and E.

Colocalization of PFNs and SMN-- On the protein level, the distribution of profilins and SMN was analyzed by immunohistochemistry (Fig. 5). HeLa cells were stained for PFN and SMN as examples of non-neuronal cells, using a monoclonal antibody specific for PFN I (mc-a-PFN I, Fig. 5, A-C). A similar distribution, diffuse in the cytoplasm and concentrated in nuclear gems, was observed for PFN I and SMN. Whereas this distribution is well known for SMN (5), the localization of PFN in gems, i.e. nuclear dots that come in pairs or in quadruplets, is a novel finding. Not all cells contained gems in their nuclei, but all gems inspected were positive for both proteins, SMN and PFN I. We further analyzed mouse spinal cord because the spinal cord is the central nervous system region affected in SMA patients. Spinal cord sections were stained with a variety of anti-profilin antibodies, in single as well as in double-labeled experiments, in combination with anti-SMN. Examples are shown in Fig. 5, D-I. High levels of PFN were seen in large anterior horn neurons using both monoclonal (Fig. 5, D-F) and polyclonal anti-PFNs (Fig. 5, G-I).

View larger version (114K): [in this window] [in a new window]   Fig. 5.   Immunocytochemical localization by double-staining of PFNs (Cy3, red) and SMN (Cy2, green). Panels A-C, cultured HeLa cells; panels D-I, frozen sections from the ventral region of mouse spinal cord. Panels A, D, and G, profilin staining with antibodies mc-a-PFN I (panel A, 2H11; Ref. 30), mc-a-PFN II (panel D, 5C6), and pc-a-PFN (panel G, Alexis). Panels B, E, and H, SMN staining with mc-a-SMN (2B1; Ref. 5). Panels C, F, and I, double exposures, to show colocalization (yellow). In all cases, there is a diffuse staining of the cytoplasm for profilin and SMN. In the non-neuronal HeLa cells (panels A-C), a nuclear gem in a quadruplet configuration is indicated by arrowheads. In panels D-F, a gem in a twin ("gemini") configuration in the nucleus of a motoneuron is indicated by arrowheads. In panels G-I, gems are indicated by arrowheads in quadruplet (upper left) and in twin (lower right) configurations. Dark area to the lower left is white matter (see Fig. 4). In all cases, gems are stained for both profilin and SMN (yellow in panels C, F, and I). Bars in panel A (valid for panels A-C) and panel G (valid for panels D-I), 10 µm.

In the many of these neurons, profilins were seen to colocalize with SMN in gems, and, like SMN, they were also distributed diffusely in the cytoplasm of the cell bodies (Fig. 5).

Again, wherever gems were seen on spinal cord sections, they were labeled for both SMN and PFN. Similar observations were made (data not shown) on a motoneuron-derived mouse cell line, NSC-19 (35).

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

In this study we show that SMN and profilins are capable of forming complexes under a variety of experimental conditions, including coimmunoprecipitation, the yeast two-hybrid system, and in vitro assays. We demonstrate that this interaction is mediated by the polyproline stretch in SMN and the corresponding polyproline binding site in profilins. Our data expand the previously known functional domain structure of SMN by a profilin-binding site, which comprises the polyproline stretches in the COOH-terminal half of SMN. Within the region of the SMN sequence delineated by amino acid residues 244-248 (the COOH-terminal Pro₅ motif), there is an overlap between the domains responsible for complex formation with the spliceosomal protein SmB (amino acids 240-267), the oligomerization domain in exon 6, and the profilin interaction domain. This suggests that profilin binding may regulate the interactions with these proteins.

Although the SMN7 deletion fragment still contains all identified binding motifs in the COOH-terminal region of the molecule, i.e. for SmB, SMN, and profilin binding, we found that this fragment failed to interact with itself as well as with PFN II in the yeast two-hybrid system. The fact that SMN7-C gave the same negative result might emphasize the importance of exon 7 for these interactions. However, we cannot exclude misfolding or degradation of this short fragment. Liu and Dreyfuss (5), however, mention an interaction between SMN and two SMN cDNA clones that lack exon 7 in their two-hybrid experiments. Because this notion is not documented by experimental data, it is difficult to explain why these results are at variance with ours. In contrast to our finding in the yeast two-hybrid system, SMN7 did bind to PFN II in solution, as seen by affinity precipitation with radioactively labeled proteins and a sensitive detection system. The discrepancy between the data obtained with the yeast two-hybrid system and those from in vitro binding studies may reflect relevant but gradual differences in the ability of SMN and SMN7 to bind profilin in the competitive environment of the cytoplasm. Alternatively, it could be caused by a diminished translation efficiency, aberrant folding, or increased sensitivity to degradation of SMN7 compared with SMN, which in itself might also be relevant for the etiology of spinal muscular atrophy. Although a variety of proline-rich ligands for the corresponding binding site in profilins has been identified (23), not all proteins with polyproline stretches interact with profilins. For example, the proline cluster containing protein CAP (36) does not bind to profilins in vitro,³ whereas its isolated polyproline peptide does so (20, 37). Moreover, the difference in affinity for proline-rich ligands between profilin I and II, as reported for VASP (20), has also been seen with SMN. These observations support the notion that the interaction between SMN and PFNs is not an unspecific consequence of the presence of polyproline stretches in SMN.

Our cytochemical studies indicate that large neurons are particularly rich in profilins. As we have shown both on the mRNA and on the protein level, PFN II is expressed predominantly in motoneurons that are the primary targets for neurodegeneration in SMA patients, whereas PFN I is expressed in many different cell types. In all cells and tissues examined, profilins were found diffusely distributed in the cytoplasm and diffusely or as dot-like structures or gems in the nucleus. The same distribution was observed for the SMN protein in HeLa cells, in mouse spinal cord (this work and Ref. 38), in neuroblastoma (38), in a motoneuron-like mouse cell line (this work), and in spinal cord tissue biopsies from human fetuses (3). A role of SMN in gem formation is suggested by the correlation of gem number in explanted fibroblasts with the severity of SMA symptoms in patients (4). Furthermore, SIP-1, a small protein that is involved in snRNP biogenesis, interacts with SMN and is also localized in gems, suggesting that SMN, SIP-1, and profilins are components of the same complex. In this context, it is noteworthy that coprecipitation of an unidentified 15-kDa polypeptide from HeLa cell lysates with either SMN or SIP-1 antibodies has been reported previously (6). We suspect this polypeptide to be profilin.

The identification of profilins as components of a physiologically important nuclear structure defines these proteins as members of a growing list of microfilament-associated proteins located in the cytoplasmic and in the nuclear compartment, with discrete functions in both locations. Such proteins comprise -catenin (39) plakoglobin (40), vinculin (41), and actin (42). Except for -catenin (43), the precise role of these proteins in the nucleus has yet to be elucidated.

It has been shown that SMN is essential for mRNA splicing (8). However, the etiology of the chromosome 5q spinal muscular atrophies, in particular its neuron-specific primary pathology, is not yet understood. The correlation between self-association of SMN protein (3, 8) and the severity of the disease strongly suggests that oligomerization of SMN is necessary for SMN's function, at least in motoneurons. Oligomerization and cytoplasmic/nuclear transport of SMN, in turn, might be modulated by profilins, especially by neuronal PFN II.

Thus our results are suggestive for the understanding of how a defect in the ubiquitous protein SMN would cause neurodegeneration while leaving other cell types unaffected (the wasting of skeletal muscle is a secondary consequence of neurodegeneration). Following this argument, PFN II might be considered as a possible target of mutations causing SMA. Genetic knockout or other manipulations of the profilin II gene of mice should provide further insights into the role of PFN II in neuronal survival.

	ACKNOWLEDGEMENTS

We thank Drs. J. Melki (Illkirch) and S. Lefebvre (Paris) for kindly providing the SMN and SMN7 cDNAs, Dr. W. Witke for the PFN II cDNA, Dr. G. Dreyfuss (Philadelphia) for a generous gift of the monoclonal antibody against SMN, Dr. U. Fischer (München) for helpful discussions, and Angela Perz (Bielefeld) for excellent technical assistance.

	FOOTNOTES

^* This work was supported in part by the Volkswagen Foundation, by the Deutsche Forschungsgemeinschaft, and by the Fonds der chemischen Industrie.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Fax: 49-521-106-5654; E-mail: h.jockusch@biologie.uni-bielefeld.de.

² W. Witke, personal communication.

³ B. M. Jockusch, unpublished data.

	ABBREVIATIONS

The abbreviations used are: SMA(s), spinal muscular atrophy(ies); SMN, survival motoneuron; PFN, profilin; sn, small nuclear; SIP, SMN-interacting protein; MBP, maltose-binding protein; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; BSA, bovine serum albumin; poly-Pro, peptides consisting of 50-60 proline residues.

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