J Biol Chem, Vol. 274, Issue 53, 38276-38276, December 31, 1999

Targeting Janus kinase 3 in mast cells prevents immediate hypersensitivity reactions and anaphylaxis.

Page 27037: Ref. 27 was incorrect and should be replaced with the following reference: Sudbeck, E., Liu, X., Narla, R., Mahajan, S., Ghosh, S., Mao, C., and Uckun, F. M. (1999) Clin. Cancer Res. 5, 1569-1582.

Also, add the following information regarding the specificity of the anti-JAK3 antibody: C-21 antibody (Santa Cruz) is directed against a 21-amino acid peptide sequence (amino acids 1105-1124) in the C-terminal portion of human JAK3. This sequence shows 67% identity to an overlapping 6-amino acid sequence (YHSLFA) in the N-terminal portion of human and mouse, as well as rat JAK3. C-21 antibody recognizes, by Western blot analysis and immunoprecipitation, recombinant wild-type mouse JAK3, which does not have the C-terminal target sequence present in human JAK3. C-21 antibody immunoprecipitates an ~120-kDa protein from the lysates of rat mast cells, which has not yet been confirmed to be JAK3 by sequence analysis but is recognized by Western blot analysis with anti-JAK3 antibodies. Further studies are underway to map the C-21 immunoreactive portions of murine and human JAK3.