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J. Biol. Chem., Vol. 275, Issue 23, 17626-17630, June 9, 2000

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Nonequivalent Nucleotide Trapping in the Two Nucleotide Binding Folds of the Human Multidrug Resistance Protein MRP1^*

Koh Nagata, Masahito Nishitani, Michinori Matsuo, Noriyuki Kioka, Teruo Amachi, and Kazumitsu Ueda

From the Laboratory of Biochemistry, Division of Applied Life Sciences, Kyoto University Graduate School of Agriculture, Kyoto 606-8502, Japan

Received for publication, January 31, 2000, and in revised form, March 3, 2000

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). P-glycoprotein hydrolyzes ATP at both NBFs, and vanadate-induced nucleotide trapping occurs at both NBFs. We examined vanadate-induced nucleotide trapping in MRP1 stably expressed in KB cell membrane by using 8-azido-[-³²P]ATP. Vanadate-induced nucleotide trapping in MRP1 was found to be stimulated by reduced glutathione, glutathione disulfide, and etoposide and to be synergistically stimulated by the presence of etoposide and either glutathione. These results suggest that glutathione and etoposide interact with MRP1 at different sites and that those bindings cooperatively stimulate the nucleotide trapping. Mild trypsin digestion of MRP1 revealed that vanadate-induced nucleotide trapping mainly occurs at NBF2. Our results suggest that the two NBFs of MRP1 might be functionally nonequivalent.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Multidrug resistance of tumor cells is a major obstacle to cancer chemotherapy. This phenomenon is frequently associated with the expression of P-glycoprotein and multidrug resistance protein 1 (MRP1),¹ both of which are ATP binding cassette (ABC) proteins. P-glycoprotein and MRP1 function as ATP-dependent efflux pumps that extrude cytotoxic drugs from the cells before they reach their intracellular targets, thus conferring resistance to many structurally dissimilar anticancer drugs, such as the Vinca alkaloids, colchicine, actinomycin D, etoposide, taxol, and anthracyclines (1-5). However, the mechanism of transport for MRP1 could be different from that for P-glycoprotein, because the depletion of intracellular glutathione (GSH) by buthionine sulfoximine results in a complete reversal of resistance to anticancer drugs of some cell lines expressing MRP1 (6, 7), but buthionine sulfoximine has no effect on P-glycoprotein-mediated multidrug resistance. It has been reported that MRP1 transports GSH-S-conjugates such as leukotriene C₄, glutathione disulfide (GSSG), and 2,4-dinitrophenyl-S-glutathione (8-11) and that MRP1 mediates ATP-dependent transport of vincristine, daunorubicin, and etoposide in the presence of GSH (12-14). From these findings, it has been postulated that MRP1 can actively cotransport GSH and unmodified xenobiotics as well as GSH-S-conjugates.

We have reported that MRP1 in membrane from a human MRP1 cDNA transformant can be specifically photoaffinity labeled with 8-azido-[-³²P]ATP by vanadate-induced nucleotide trapping (15). Vanadate and Mg²⁺ were required for trapping of nucleotide, and photoaffinity labeling was inhibited by the excess ADP as well as ATP. These results have suggested that a stable inhibitory complex MRP1·MgADP·Vi, an analog of the MRP1·MgADP·P_i transition state complex, is formed in the presence of vanadate, as suggested for P-glycoprotein (16). Vanadate-induced nucleotide trapping in P-glycoprotein has been reported to be stimulated by the transport substrates (17, 18) and to occur nonselectively in both nucleotide-binding folds (NBFs) (19). Because vanadate-induced trapping with 8-azido-ATP in MRP1 was stimulated in the presence of GSH and GSSG as well as anticancer drugs, we assumed that these compounds directly interact with MRP1 and stimulate the formation of the transition state in the ATPase reaction of MRP1 (15). However, we have not been able to observe the cooperative effect of these compounds on vanadate-induced nucleotide trapping, which might be expected if MRP1 should actively cotransport GSH and anticancer drugs. Chang et al. (20) reported that ATPase activity of purified MRP1 was stimulated by GSH and doxorubicin and that their effects were additive; however, the stimulatory effect of GSH and doxorubicin on MRP1 ATPase was 1.7-fold at most. In this study, we examined the vanadate-induced nucleotide trapping in MRP1 at 26 °C, and found that etoposide and GSH (or GSSG) synergistically stimulate the nucleotide trapping as much as 20-fold. Interestingly, mild trypsin digestion revealed that vanadate-induced nucleotide trapping mainly occurs at NBF2.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Materials-- The monoclonal antibodies MRPr1 (21) and MRPm6 (22) were purchased from Nichirei and Chemicon, respectively. Etoposide was from Sigma and Bristol Myers Squibb. 8-Azido-[-³²P]ATP was purchased from ICN Biomedicals, and [³H]17-estradiol 17--D-glucuronide (E217G) (44.0 µCi/nmol) was from NEN Life Science Products.

Vanadate-induced Nucleotide Trapping in MRP1 with 8-Azido-[-³²P]ATP-- Membranes (10 µg) were prepared from KB/MRP1, a human MRP1 cDNA transformant as described previously (15). They were mixed with GSH and etoposide in 6 µl of TEM buffer (40 mM Tris-Cl (pH 7.5), 3 mM MgSO₄, 0.1 mM EGTA) containing 200 µM sodium orthovanadate and 2 mM ouabain on ice, and 10 µM 8-azido-[-³²P]ATP was added as a final component. The mixture was incubated for 1 min at 26 °C. The reactions were stopped by the addition of 500 µl of ice-cold TEM buffer, and free ATP was removed after centrifugation (15,000 × g, 5 min, 2 °C). The pellets were washed in the same buffer, resuspended in 8 µl of TEM buffer, and irradiated for 45 s (at 254 nm, 8.2 mW/cm²) on ice. The samples were electrophoresed on a 7% SDS-polyacrylamide gel and autoradiographed. The trapped 8-azido-[-³²P]ATP in MRP1 was measured by scanning with a radioimaging analyzer (BAS2000, Fuji Photo Film Co.). Experiments were done at least in triplicate.

Limited Trypsin Digestion of MRP1-- The photoaffinity-labeled membranes (20 µg) were resuspended in TEM buffer containing 0.25-2.0 µg/ml trypsin and 250 mM sucrose to 1.7 µg of membrane proteins/µl and incubated for 15 min at 20 °C.

Transport Studies-- Membrane vesicles were prepared from KB-3-1 or KB/MRP1 as described previously (23), and the transport studies were done using the rapid filtration technique (24). Membrane vesicle suspension (10 µg of protein) was incubated with 57 nM [³H]E217G in 20 µl of transport medium (10 mM Tris, 250 mM sucrose, 100 mM NaCl, 10 mM MgCl₂, pH 7.4) for 30 min at 37 °C.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Time Course of Vanadate-induced Nucleotide Trapping in MRP1-- We examined vanadate-induced nucleotide trapping in MRP1 stably expressed in KB cell membrane by using 8-azido-[-³²P]ATP. It has been reported that, when P-glycoprotein is incubated with ATP and vanadate, the inhibition of P-glycoprotein ATPase activity induced by vanadate is rapid, reaching completion in less than 10 s at 37 °C (25). Because we assumed that the reaction of MRP1 would be as fast as P-glycoprotein and the reaction at 37 °C would be too fast to be analyzed quantitatively, we examined the time course of vanadate-induced nucleotide trapping in MRP1 at 26 °C. When membranes from KB/MRP1 were incubated with vanadate, Mg²⁺, and 10 µM 8-azido-[-³²P]ATP, followed by UV irradiation after removing free ligands, a 190-kDa protein was specifically photoaffinity-labeled in a time-dependent manner (Fig. 1A, lanes 1-4). The 190-kDa protein was not labeled in membrane proteins from untransfected KB-3-1 (data not shown), indicating that the 190-kDa protein is MRP1. In the presence of 2 mM GSSG, which is considered to be one of the endogenous substrates for MRP1 (9), photoaffinity labeling of a 190-kDa protein was stimulated (Fig. 1A, lanes 5-8) and showed a strong time dependence up to 2 min (Fig. 1B).

View larger version (34K): [in this window] [in a new window]   Fig. 1.   Time dependence of vanadate-induced nucleotide trapping of MRP1. A, membranes (10 µg) from KB/MRP1 were incubated with 10 µM 8-azido-[-32P]ATP containing 200 µM orthovanadate and 3 mM MgSO4 in the absence (lanes 1-4) or presence (lanes 5-8) of 2 mM GSSG at 26 °C for 0.5 min (lanes 1 and 5), 1 min (lanes 2 and 6), 2 min (lanes 3 and 7), and 4 min (lanes 4 and 8). After free ATP was removed, the proteins were irradiated with UV and analyzed as described under "Experimental Procedures." B, trapped 8-azido-[-32P]ATP in MRP1 in the absence () or presence of GSSG () was measured and expressed relative to that at 1 min in the absence of GSSG.

Effects of Etoposide and Glutathione on Nucleotide Trapping in MRP1-- We examined the effects of GSH, GSSG, and etoposide on the nucleotide trapping in KB/MRP1 membranes incubated at 26 °C for 1 min with 10 µM 8-azido-[-³²P]ATP (Fig. 2). KB/MRP1 cells showed the highest resistance to etoposide among the anticancer drugs examined (15). GSSG, GSH, and etoposide significantly stimulated nucleotide trapping in a concentration-dependent manner. The nucleotide trapping is stimulated 4.3- and 2.2-fold by 2 mM GSSG and GSH, respectively, and 3.7-fold by 1 mM etoposide.

View larger version (23K): [in this window] [in a new window]   Fig. 2.   Dependence of vanadate-induced nucleotide trapping of MRP1 on the concentration of GSH, GSSG, and etoposide. Membranes (10 µg) from KB/MRP1 were incubated with 10 µM 8-azido-[-32P]ATP and 200 µM orthovanadate and 3 mM MgSO4 in the presence of GSH (), GSSG (), and etoposide () at 26 °C for 1 min. After free ATP was removed, proteins were irradiated with UV and analyzed as described under "Experimental Procedures." Trapped 8-azido-[-32P]ATP in MRP1 was expressed relative to that in the absence of glutathione or etoposide.

Synergistic Effects of Etoposide and Glutathione on Nucleotide Trapping in MRP1-- The unmodified xenobiotics have been suggested to be actively cotransported with GSH (12, 13). If this is true, then GSH and xenobiotics can be expected to cooperatively affect ATP hydrolysis of MRP1. To explore this possibility, we examined the effect of etoposide on the nucleotide trapping in the presence of GSH. Fig. 3A shows that etoposide at 0.5 mM and 1 mM stimulates the nucleotide trapping about 20-fold in the presence of 2 mM GSH. Interestingly, in the presence of 2 mM GSSG, etoposide also stimulates the nucleotide trapping to the similar extent. The combination of 2 mM GSH and 2 mM GSSG did not stimulate nucleotide trapping more than 2 mM GSSG alone did (data not shown). No stimulatory effect on the nucleotide trapping with etoposide was noted with 2-mercaptoethanol or dithiothreitol, which are other reducing agents (data not shown).

View larger version (37K): [in this window] [in a new window]   Fig. 3.   Synergistic stimulation by glutathione and etoposide. A, membranes (10 µg) from KB/MRP1 were incubated with 10 µM 8-azido-[-32P]ATP and 200 µM orthovanadate and 3 mM MgSO4 in the presence of etoposide (), 2 mM GSH and etoposide (), and 2 mM GSSG and etoposide () at 26 °C for 1 min. After free ATP was removed, proteins were irradiated with UV and analyzed as described under "Experimental Procedures". Trapped 8-azido-[-32P]ATP in MRP1 was expressed relative to that in the absence of glutathione or etoposide. B and C, membranes (10 µg) from KB/MRP1 were incubated with 10 µM 8-azido-[-32P]ATP, 200 µM orthovanadate, and 3 mM MgSO4 in the presence of 2 mM GSH and 1 mM etoposide at 26 °C (B) or at 37 °C (C) for 1 min. Lane 1, without GSH or etoposide; lane 3, without MgSO4 in the presence of 1 mM EDTA; lane 4, without vanadate; lane 5, in the presence of 1 mM ATP; lane 6, in the presence of 1 mM ADP. After free ligands were removed, proteins were irradiated with UV and analyzed.

In the absence of Mg²⁺ or vanadate, and in the presence of excess ATP, nucleotide trapping was reduced by 98%, 97%, and 96%, respectively at 26 °C (Fig. 3B). Excess ADP also reduced the nucleotide trapping, but the inhibitory effect (85%) was less than ATP at 26 °C (lane 6). At 37 °C, excess ADP reduced nucleotide trapping as efficiently as excess ATP did (94% by ADP and 98% by ATP) (Fig. 3C).

Mild Trypsin Digestion of MRP1-- The cooperative stimulation of the nucleotide trapping by GSH and etoposide suggested that they interact with MRP1 at different sites as proposed (26) and that those bindings stimulate the nucleotide trapping cooperatively. Therefore, we assumed that these bindings might have different effects on the two NBFs of MRP1. For example, GSH binding might stimulate ATP hydrolysis at one of the two NBFs, and etoposide binding might stimulate it at the other.

Mild trypsin digestion of MRP1 has been reported to produce two large polypeptides (21). One is a fragment of approximately 120 kDa, corresponding to the N-proximal half of MRP1, and the second is a fragment of 75-80 kDa, corresponding to the C-proximal half of MRP1. It is supposed that the 120-kDa fragment contains NBF1 and the 75- to 80-kDa fragment contains NBF2, because they react with antibodies against NBF1 and NBF2, respectively (21). To examine which NBF is photoaffinity-labeled, photoaffinity-labeled MRP was digested with trypsin.

KB/MRP1 membranes were incubated with 10 or 100 µM 8-azido-[-³²P]ATP and vanadate in the presence or absence of GSH and etoposide at 26 °C for 1 min. Then, membrane proteins were photoaffinity-labeled by UV irradiation after removing free ligands and mildly digested with 2.0 µg/ml trypsin for 15 min at 20 °C. The mild trypsin digestion produced strongly photoaffinity-labeled 75- to 80-kDa fragments and a faintly labeled 120-kDa fragment under any conditions examined (GSSG alone, etoposide alone, and etoposide with GSH) (Fig. 4A). The photoaffinity-labeled 120-kDa fragment was observed slightly better with 100 µM 8-azido-[-³²P]ATP than with 10 µM 8-azido-[-³²P]ATP. However, the 75- to 80-kDa fragments were mainly photoaffinity-labeled under any conditions examined.

View larger version (56K): [in this window] [in a new window]   Fig. 4.   Limited tryptic digestion of MRP1 after photoaffinity labeling. Membranes (20 µg) from KB/MRP1 were incubated with 10 µM (lanes 1, 3, 5, 7) or 100 µM (lanes 2, 4, 6, 8) 8-azido-[-32P]ATP in the presence of 200 µM orthovanadate and 3 mM MgSO4. The reactions were done in the absence (lanes 1 and 2) or presence of 1 mM etoposide (lanes 3 and 4), 2 mM GSSG (lanes 5 and 6), and 2 mM GSH and 1 mM etoposide (lanes 7 and 8) at 26 °C for 1 min. After free ATP was removed, the proteins were irradiated with UV and digested with 2.0 µg/ml trypsin for 15 min at 20 °C. B, Western blot with mAb MRPr1 (lanes 1 and 2) and with mAb MRPm6 (lanes 3 and 4) of membrane proteins from KB/MRP1 digested with 2.0 µg/ml trypsin before (lanes 1 and 3) and after (lanes 2 and 4) vanadate-induced nucleotide trapping. Lane 5, photoaffinity labeling under the condition of A, lane 7. The tryptic 120-kDa fragment containing NBF1 and the 75- to 80-kDa fragments containing NBF2 are indicated.

Western blotting (Fig. 4B) indicates that the 120-kDa fragment, produced by the mild trypsin digestion, was recognized by monoclonal antibody (mAb) MRPr1, and the 75- to 80-kDa fragments were recognized by mAb MRPm6 as reported previously (21). MRPr1 and MRPm6 have been reported to recognize the N-proximal half of MRP1 containing NBF1 and the C-proximal half containing NBF2, respectively (21). Photoaffinity labeling, done in parallel, showed that the labeled 75- to 80-kDa fragments comigrated with the fragments recognized by MRPm6 (lane 5). These results suggest that NBF2 of MRP1 is preferentially photoaffinity-labeled.

Preferential Vanadate-induced Nucleotide Trapping at NBF2 of MRP1-- To confirm that the 75- to 80-kDa fragments, containing NBF2, were preferentially photoaffinity-labeled, the decrease of photoaffinity-labeled undigested MRP1 and the increase of labeled tryptic fragments were measured. KB/MRP1 membranes were incubated with 10 µM 8-azido-[-³²P]ATP and vanadate in the presence of GSH and etoposide at 26 °C and were photoaffinity-labeled by UV irradiation after removing free ligands. They were then incubated with various concentrations of trypsin for 15 min at 20 °C (Fig. 5).

View larger version (35K): [in this window] [in a new window]   Fig. 5.   Preferential vanadate-induced nucleotide trapping at NBF2 of MRP1. A, membrane proteins (20 µg) from KB/MRP1 were incubated with 10 µM 8-azido-[-32P]ATP, 200 µM orthovanadate, and 3 mM MgSO4 in the presence of 2 mM GSH and 1 mM etoposide for 3 min at 26 °C and UV irradiated after removing free ATP. Membrane proteins were digested with 0 (lane 1), 0.25 (lane 2), 1 (lane 3), and 2 µg/ml trypsin (lane 4) for 15 min at 20 °C and the tryptic fragments were analyzed. B, relative photoaffinity labeling of undigested MRP1 (), the tryptic 120-kDa fragment containing NBF1 (), and the 75- to 80-kDa fragments containing NBF2 () were calculated relative to the photoaffinity labeling of undigested MRP1 without trypsin digestion.

The photoaffinity-labeled band of undigested MRP1 decreased and those of tryptic fragments increased with increasing concentrations of trypsin (Fig. 5B). By incubating with 0.25 µg/ml trypsin, the photoaffinity-labeled band of undigested MRP1 decreased 24 ± 6%, and those of the 120- and 75- to 80-kDa tryptic fragments increased 3 ± 2% and 20 ± 4%, respectively. This indicates that most of 8-azido-[-³²P]ATP is trapped in the 75- to 80-kDa fragments containing NBF2 after incubation in the presence of GSH and etoposide.

8-Azido-ATP Supports Active Transport of E217G-- To confirm that 8-azido-ATP supports the active transport activity of MRP, we examined ATP-dependent E217G uptake into membrane vesicles prepared from KB/MRP1 (Fig. 6). E217G has been reported to be a good substrate for MRP1 (27). [³H]E217G uptake into membrane vesicles prepared from KB/MRP1 was observed in the presence of 8-azido-ATP as well as ATP, but not in the presence of AMP or ADP. The ATP-dependent uptake of [³H]E217G into membrane vesicles prepared from KB-3-1 host cells was less than one-tenth of that with membrane vesicles prepared from KB/MRP1. These results suggest that 8-azido-ATP is hydrolyzed by MRP1 and supports active transport by MRP1.

View larger version (19K): [in this window] [in a new window]   Fig. 6.   8-Azido-ATP supports the uptake of [3H]E217G into membrane vesicles. Membrane vesicles prepared from KB-3-1 or KB/MRP1 were incubated with 57 nM [3H]E217G in the presence of no nucleotide (lanes 1 and 6), 5 mM AMP (lanes 2 and 7), 5 mM ADP (lanes 3 and 8), 5 mM ATP (lanes 4 and 9), or 5 mM 8-Azido-ATP (lanes 5 and 10) for 30 min. Experiments were done in triplicate and expressed relative to the value with KB/MRP1 in the presence of ATP.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

We demonstrated in the present study that MRP1 is specifically photoaffinity-labeled with 8-azido-[-³²P]ATP in KB/MRP1 membranes. The photoaffinity labeling of MRP is dependent on vanadate and Mg²⁺ and is inhibited by the excess ADP as well as ATP. These properties of vanadate-induced nucleotide trapping is similar to those of P-glycoprotein (25). 8-Azido-ATP supports active transport of E217G into plasma membrane vesicles from KB/MRP1 (Fig. 6). 8-Azido-ATP also supports active transport of leukotriene C₄ with similar an apparent K_m for ATP (28). These results suggest that a stable inhibitory complex, MRP1·MgADP·Vi, an analog of the MRP1·MgADP·P_i transition state complex, is formed in the presence of vanadate after ATP hydrolysis, as suggested for P-glycoprotein (16). However, more experiments are needed to establish whether there is a good correlation between vanadate-induced nucleotide trapping and ATP hydrolysis by MRP.

The vanadate-induced nucleotide trapping of MRP1 with 8-azido-[-³²P]ATP is stimulated by GSH, GSSG, and etoposide, and, importantly, is synergistically stimulated by both GSH and etoposide and by GSSG and etoposide. These results suggest that glutathione and etoposide interact with MRP1 at different sites as proposed (26) and that those bindings cooperatively stimulate the nucleotide trapping.

GSSG is transported by MRP1 (29), whereas transport of GSH is controversial (6, 29, 30). Lower stimulation of vanadate-induced nucleotide trapping in MRP1 by GSH compared with GSSG suggests that GSH per se is not a good substrate for MRP1. Synergistic stimulation of vanadate-induced nucleotide trapping by etoposide and GSH suggests that their simultaneous binding to MRP1 cooperatively induces conformational changes at the catalytic NBF. Because GSSG is as effective as GSH for stimulating vanadate-induced nucleotide trapping in the presence of etoposide, and because GSSG would not interact with etoposide even in a noncovalently associated form, etoposide may be recognized as an unmodified form by MRP together with glutathione as proposed (6, 30). We have examined the effects of doxorubicin and vincristine on vanadate-induced nucleotide trapping in MRP. However, they did not show such strong synergistic effects with glutathione as etoposide showed (data not shown). The reason why etoposide shows such a strong synergistic effect with glutathione remains to be identified.

In P-glycoprotein, two NBFs appear to be functionally equivalent (16). Both NBFs can hydrolyze ATP, and vanadate-induced nucleotide trapping occurs in both NBFs probably nonselectively (19) and sequentially (16, 31). Mild trypsin digestion of MRP1 reveals that vanadate-induced nucleotide trapping occurs mainly at NBF2 under any conditions examined: under a basal condition, in the presence of GSSG alone, etoposide alone, and etoposide and GSH. There could be several possible explanations for the preferential vanadate-induced nucleotide trapping at NBF2: the first is that NBF1 has lower ATP binding affinity than does NBF2. If that is the case, NBF1 might not be photoaffinity-labeled with 10 µM 8-azido-[-³²P]ATP. However, NBF2 was preferentially photoaffinity-labeled even with 100 µM 8-azido-[-³²P]ATP. The second explanation is that MRP1 hydrolyzes ATP only at NBF2; the third is that ATP hydrolysis of MRP1 occurs selectively at NBF2 first, when substrates bind to MRP, and then at NBF1. If the first catalytic turnover occurs selectively at NBF2 of MRP1, it would produce an intermediate containing ADP at NBF2, and then vanadate would bind to this intermediate to form a stable inhibitory complex, MRP1·MgADP·Vi at NBF2. This would not allow any further reaction at NBF1, and no vanadate-induced nucleotide trapping would occur at NBF1. The fourth explanation would be that NBF1 of MRP1 also hydrolyzes ATP but a stable inhibitory complex, MRP1·MgADP·Vi, is not formed at NBF1. It is not clear yet what causes the preferential photoaffinity labeling of NBF2. However, these results suggest that the two NBFs of MRP1 are functionally nonequivalent.

Nonequivalent features between two NBFs of ABC proteins have been reported for SUR1 and cystic fibrosis transmembrane conductance regulator (CFTR). SUR1 is a subunit of the pancreatic -cell K_ATP channel, which plays a key role in the regulation of glucose-induced insulin secretion. SUR1 may not be a transporter or a channel but may function only as a switch that monitors intracellular ATP/ADP concentrations. SUR1 binds ATP strongly at NBF1 even in the absence of Mg²⁺ and binds MgADP at NBF2 (17, 32). Recently, we suggested that NBF2 of SUR1 may hydrolyze ATP (32, 33). CFTR is an ATP-dependent chloride channel and malfunctions in cystic fibrosis. NBF1 of CFTR was preferentially labeled with 8-azido-ATP in the absence of vanadate. The addition of vanadate increased photoaffinity labeling and resulted in the labeling of both NBFs of CFTR (34).

In conclusion, vanadate-induced nucleotide trapping in MRP1 is strongly and synergistically stimulated by the presence of etoposide and glutathione. Interestingly, vanadate-induced nucleotide trapping occurs mainly at NBF2, suggesting the nonequivalency of two NBFs of MRP1. This new finding on variation in the cooperativity of the two NBFs in ABC proteins should promote understanding of the molecular basis of the differences in function of the various ABC proteins as transporters, ion channels, or ion channel regulators.

	ACKNOWLEDGEMENTS

We thank Drs. S. P. Cole and R. G. Deeley for communicating data in advance of publication. We thank Dr. G. J. R. Zaman, the Netherlands Cancer Institute, for the gift of MRP1 cDNA. We thank Dr. Hiroshi Suzuki, the University of Tokyo, for the technical advice and Judy Noguchi for correcting English.

	FOOTNOTES

^* This work was supported by Grants-in-Aid for Scientific Research on Priority Areas "ABC Proteins" (Grant 10217205) from the Ministry of Education, Science, Sports, and Culture of Japan and by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Laboratory of Biochemistry, Dept. of Applied Life Science, Kyoto University Graduate School of Agriculture, Kyoto 606-8502, Japan. Tel.: 81-75-753-6105; Fax: 81-75-753-6104; E-mail: uedak @kais.kyoto-u.ac.jp.

Published, JBC Papers in Press, March 29, 2000, DOI 10.1074/jbc.M000792200

	ABBREVIATIONS

The abbreviations used are: MRP, multidrug resistance-associated protein; ABC, ATP-binding cassette; NBF, nucleotide-binding fold; GSH, glutathione; GSSG, glutathione disulfide; CFTR, cystic fibrosis transmembrane conductance regulator; SUR, sulfonylurea receptor; E217G, 17-estradiol 17--D-glucuronide; mAb, monoclonal antibody.

	REFERENCES

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				L. F. Payen, M. Gao, C. J. Westlake, S. P. C. Cole, and R. G. Deeley Role of Carboxylate Residues Adjacent to the Conserved Core Walker B Motifs in the Catalytic Cycle of Multidrug Resistance Protein 1 (ABCC1) J. Biol. Chem., October 3, 2003; 278(40): 38537 - 38547. [Abstract] [Full Text] [PDF]

				C. Basso, P. Vergani, A. C. Nairn, and D. C. Gadsby Prolonged Nonhydrolytic Interaction of Nucleotide with CFTR's NH2-terminal Nucleotide Binding Domain and its Role in Channel Gating J. Gen. Physiol., August 25, 2003; 122(3): 333 - 348. [Abstract] [Full Text] [PDF]

				R. Yang, L. Cui, Y.-x. Hou, J. R. Riordan, and X.-b. Chang ATP Binding to the First Nucleotide Binding Domain of Multidrug Resistance-associated Protein Plays a Regulatory Role at Low Nucleotide Concentration, whereas ATP Hydrolysis at the Second Plays a Dominant Role in ATP-dependent Leukotriene C4 Transport J. Biol. Chem., August 15, 2003; 278(33): 30764 - 30771. [Abstract] [Full Text] [PDF]

				G-T Ho, F M Moodie, and J Satsangi Multidrug resistance 1 gene (P-glycoprotein 170): an important determinant in gastrointestinal disease? Gut, May 1, 2003; 52(5): 759 - 766. [Abstract] [Full Text]

				Y.-x. Hou, J. R. Riordan, and X.-b. Chang ATP Binding, Not Hydrolysis, at the First Nucleotide-binding Domain of Multidrug Resistance-associated Protein MRP1 Enhances ADP{middle dot}Vi Trapping at the Second Domain J. Biol. Chem., January 31, 2003; 278(6): 3599 - 3605. [Abstract] [Full Text] [PDF]

				R. J. Pezza, M. A. Villarreal, G. G. Montich, and C. E. Argarana Vanadate inhibits the ATPase activity and DNA binding capability of bacterial MutS. A structural model for the vanadate-MutS interaction at the Walker A motif Nucleic Acids Res., November 1, 2002; 30(21): 4700 - 4708. [Abstract] [Full Text] [PDF]

				L. Aleksandrov, A. A. Aleksandrov, X.-b. Chang, and J. R. Riordan The First Nucleotide Binding Domain of Cystic Fibrosis Transmembrane Conductance Regulator Is a Site of Stable Nucleotide Interaction, whereas the Second Is a Site of Rapid Turnover J. Biol. Chem., May 3, 2002; 277(18): 15419 - 15425. [Abstract] [Full Text] [PDF]

				A. L. Davidson Mechanism of Coupling of Transport to Hydrolysis in Bacterial ATP-Binding Cassette Transporters J. Bacteriol., March 1, 2002; 184(5): 1225 - 1233. [Full Text] [PDF]

				C. Vigano, M. Julien, I. Carrier, P. Gros, and J.-M. Ruysschaert Structural and Functional Asymmetry of the Nucleotide-binding Domains of P-glycoprotein Investigated by Attenuated Total Reflection Fourier Transform Infrared Spectroscopy J. Biol. Chem., February 8, 2002; 277(7): 5008 - 5016. [Abstract] [Full Text] [PDF]

				Y.-x. Hou, L. Cui, J. R. Riordan, and X.-b. Chang ATP Binding to the First Nucleotide-binding Domain of Multidrug Resistance Protein MRP1 Increases Binding and Hydrolysis of ATP and Trapping of ADP at the Second Domain J. Biol. Chem., February 8, 2002; 277(7): 5110 - 5119. [Abstract] [Full Text] [PDF]

				Y.-M. Qian, W. Qiu, M. Gao, C. J. Westlake, S. P. C. Cole, and R. G. Deeley Characterization of Binding of Leukotriene C4 by Human Multidrug Resistance Protein 1. EVIDENCE OF DIFFERENTIAL INTERACTIONS WITH NH2- AND COOH-PROXIMAL HALVES OF THE PROTEIN J. Biol. Chem., October 12, 2001; 276(42): 38636 - 38644. [Abstract] [Full Text] [PDF]

				E. M. Leslie, Q. Mao, C. J. Oleschuk, R. G. Deeley, and S. P. C. Cole Modulation of Multidrug Resistance Protein 1 (MRP1/ABCC1) Transport and ATPase Activities by Interaction with Dietary Flavonoids Mol. Pharmacol., April 16, 2001; 59(5): 1171 - 1180. [Abstract] [Full Text]

				Q. Mao, R. G. Deeley, and S. P. C. Cole Functional Reconstitution of Substrate Transport by Purified Multidrug Resistance Protein MRP1 (ABCC1) in Phospholipid Vesicles J. Biol. Chem., October 27, 2000; 275(44): 34166 - 34172. [Abstract] [Full Text] [PDF]

				M. Matsuo, K. Tanabe, N. Kioka, T. Amachi, and K. Ueda Different Binding Properties and Affinities for ATP and ADP among Sulfonylurea Receptor Subtypes, SUR1, SUR2A, and SUR2B J. Biol. Chem., September 8, 2000; 275(37): 28757 - 28763. [Abstract] [Full Text] [PDF]

				A. R. Walmsley, T. Zhou, M. I. Borges-Walmsley, and B. P. Rosen A Kinetic Model for the Action of a Resistance Efflux Pump J. Biol. Chem., February 23, 2001; 276(9): 6378 - 6391. [Abstract] [Full Text] [PDF]

K. M. Kerr, Z. E. Sauna, and S. V. Ambudkar Correlation between Steady-state ATP Hydrolysis and Vanadate-induced ADP Trapping in Human P-glycoprotein. EVIDENCE FOR ADP RELEASE AS THE RATE-LIMITING STEP IN THE CATALYTIC CYCLE AND ITS MODULATION BY SUBSTRATES J. Biol. Chem., March 16, 2001; 276(12): 8657 - 8664. [Abstract] [Full Text] [PDF]