J Biol Chem, Vol. 275, Issue 3, 2246-2246, January 21, 2000

Transient, phorbol ester-induced DOC2-Munc13 inter- actions in vivo.

Page 27350: The final sentence under "Results," "However, a similar pretreatment of the cells with the Cl domain-specific PKC inhibitor chelerythrine (which does block PE-induced Munc13-1 translocation (1)) abolished the translocation effect for all construct combinations (Fig. 4, e and f)," should read: "However, a similar pretreatment of the cells with the PKC inhibitor chelerythrine (which does block PE-induced Munc13-1 translocation (R. R. Duncan, unpublished observation)) abolished the translocation effect for all construct combinations (Fig. 4, e and f)."

This change clarifies the point that chelerythrine may not be a specific inhibitor of Cl domain-mediated PKC effects. The reference cited (Betz, A., Ashery, U., Rickmann, M., Augustin, I., Neher, E., Sudhof, T. C., Rettig, J., and Brose, N. (1998) Neuron 21, 123-136) used calphostin C as a Cl domain inhibitor, not chelerythrine. We have additional data using a range of PKC inhibitors, including calphostin C and staurosporine, which further confirm our published data but which are not presented with this work.

Page 27347, "abbreviations" footnote: Correct the definition of "DOCZ, double CZ" to "DOC2, double C2."