HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 40, 37529-37536, October 5, 2001

This Article Abstract Full Text (PDF) All Versions of this Article: 276/40/37529    most recent M103234200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foehr, E. D. Articles by Bradshaw, R. A. Search for Related Content PubMed PubMed Citation Articles by Foehr, E. D. Articles by Bradshaw, R. A. Social Bookmarking                      What's this?

The Role of Tyrosine Residues in Fibroblast Growth Factor Receptor 1 Signaling in PC12 Cells

SYSTEMATIC SITE-DIRECTED MUTAGENESIS IN THE ENDODOMAIN^*

Erik D. Foehr^§, Simona Raffioni^¶, Judith Murray-Rust, and Ralph A. Bradshaw^**

From the Departments of  Physiology and Biophysics, ^** Anatomy and Neurobiology, and ^¶ Biological Chemistry, School of Medicine, University of California, Irvine, California 92697, ^§ Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94103, and the  Imperial Cancer Research Fund, Lincolns Inn Fields, London WC2A 3PX, United Kingdom

Received for publication, April 11, 2001, and in revised form, June 20, 2001

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

To assess the contribution of the intracellular domain tyrosine residues to the signaling capacity of fibroblast growth factor receptor 1 (FGFR1), stably transfected chimeras bearing the ectodomain of the platelet-derived growth factor receptor (PDGFR) and the endodomain of FGFR1 were systematically altered by a tyrosine to phenylalanine bloc and individual conversions. The 15 tyrosine residues of the endodomain of this construct (PFR1) were divided into four linear segments (labeled A, B, C, and D) that contained 4, 4, 2, and 5 tyrosine residues, respectively. When stimulated by platelet-derived growth factor, derivatives in which the A, B, or A + B blocs of tyrosines were mutated were about two-thirds as active as the unmodified chimera at 48 h but achieved full activity by 96 h in a neurite outgrowth assay in transfected PC12 cells. Elimination of only the two activation loop tyrosines (C bloc) also inactivated the receptor. All derivatives in which 4 (or 5) of the D bloc tyrosines were mutated were inactive in producing differentiation but showed low levels of kinase activity in in vitro assays. Derivatives in which 1, 2, or 3 tyrosines of the D bloc in different combinations were systematically changed demonstrated that 2 residues (Tyr⁶⁷⁷ and Tyr⁷⁰¹, using hFGFR1 numbering) were essential for bioactivity, but the remaining 3 residues, including Tyr⁷⁶⁶, the previously identified site for phospholipase C (PLC) activation, were not. Differentiation activity was paralleled by the activation (phosphorylation) of FRS2, SOS, and ERK1/2. PLC activity was dependent on the presence of Tyr⁷⁶⁶ but also required Tyr⁶⁷⁷ and/or Tyr⁷⁰¹. Although fully active chimeras did not require PLC, the responses of chimeras showing reduced activation of FRS2 were significantly enhanced by this activity. These results establish that PFR1 does not utilize any tyrosine residues, phosphorylated or not, to activate FRS2. However, it does require Tyr⁶⁷⁷ and/or Tyr⁷⁰¹, which may function to stabilize the active conformation directly or indirectly.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Although divergent in detail, the receptor tyrosine kinase (RTK)¹ family is characterized by a similar organization in which the protomeric unit is composed of a ligand binding (ecto) domain, a single pass transmembrane region, and an intracellular (endo) domain featuring an activatable tyrosine kinase. Activation generally requires ligand binding and a stabilized dimeric structure that allows interaction between the constituent monomers and trans protomer autophosphorylation (1, 2). The modifications (tyrosine phosphorylations) introduced usually contribute to stabilizing the activated form of the kinase and can provide docking sites for downstream transducers and effectors. However, not all RTKs require tyrosine phosphorylation for activation, e.g. the EGFR (3), and some of these modifications apparently do not contribute directly to functional responses.

The kinase domains of RTKs are homologs of the larger kinase family and show the characteristic bilobal structure of these enzymes (2). ATP and the protein (peptide) substrate are bound in a cleft formed by the two domains, and several key binding/catalytic residues are universally conserved (1). Generally, RTK activation is controlled by a loop (or "lip") that contains one to three tyrosine residues that are sites of phosphorylation. This activation loop (A-loop) is apparently quite flexible and can move readily from a conformation in which it occludes all or part of the cleft to one in which substrate binding is not impeded. In most cases, phosphorylation of the A-loop tyrosines clearly stabilizes the "active" conformation, but it is less clear how ligand binding shifts the equilibrium from the "closed" to "open" form prior to the covalent modification.

The three-dimensional structures of several kinases, including those of the insulin receptor (IR) and fibroblast growth factor receptor 1(FGFR1), have been particularly valuable in developing an understanding of kinase activation mechanisms (4, 5). The unphosphorylated forms of the FGFR1 and the IR kinase (IRK) domains show quite different orientations of the A-loop, although both occupy the active site cleft to some degree. In the unphosphorylated IRK, both the ATP and protein substrate binding sites are blocked, whereas in the unphosphorylated FGFR1K, an ATP analog is bound but residues of the catalytic loop hinder binding of peptide substrates. So far, there are structures available only for the IRK in both the inhibited and activated forms. There are large changes in conformation between inactive IRK and the tri-phosphorylated structure (4). In the A-loop of the unphosphorylated IRK structure, Tyr¹¹⁶² is H-bonded to the putative catalytic bases and blocks the nucleotide binding site. In this tri-phosphorylated form of IRK, pTyr¹¹⁶³ contributes to stabilization of the activated A-loop conformation by interaction with Arg^1155, and the phosphate group of pTyr¹¹⁶² H-bonds to the side chain of Arg¹¹⁶⁴. In FGFR1, mutation of either tyrosine in the A-loop sequence (Tyr⁶⁵³ or Tyr⁶⁵⁴), which are analogous to Tyr¹¹⁶² and Tyr¹¹⁶³ of IR, does not inactivate the enzyme (although mutation of both does) (5). It is likely, therefore, that both tyrosines interact, in the phosphorylated state, to stabilize the open form of the A-loop.

There are four major forms of the FGFR (designated FGFR1-4) that are members of the RTK family. Their expression is widely distributed in terms of tissues and developmental periods, reflecting a variety of cellular activities (6). All of the principal isoforms activate phospholipase C (PLC) and the RAS/MAPK (ERK) pathways, albeit with varying effectiveness (7). The former is bound to a specific phosphotyrosine (Tyr⁷⁷⁶ in FGFR1) found in the same relative position in all of the isoforms (6), and mutation of this residue (usually to phenylalanine) results in complete loss of PLC-dependent responses (8, 9). The induction of ERK1/2 activation (via activated RAS) also occurs through the phosphorylation of FRS2, a signaling scaffold that binds constitutively to FGFRs regardless of their activation state (10-13). FRS2 has multiple sites for phosphorylation, which when modified allow binding of Grb2 and Shp2 leading to subsequent ERK activation as well as other responses (13).

The interaction of FRS2 with FGFR1 occurs via the binding of the phosphotyrosine binding domain of FRS2 to a 12-residue segment in the juxtamembrane region of FGFR1 (14). Because the other FGFRs also activate FRS2 and contain this sequence, a similar interaction is likely with these as well. Importantly, this segment does not contain a tyrosine residue, and hence FRS2 interaction and activation apparently do not depend directly on the formation of a phosphotyrosine derivative. FGFRs also induce Shc phosphorylation (10), although direct interactions with the receptor have not been observed and, at least in PC12 cell differentiation, dominant negative derivatives have demonstrated that it does not play a role in differentiation (6).

Seven tyrosine residues in the FGFR1 endodomain are phosphorylated in the activated receptor (15). In addition to the two found in the A-loop (Tyr⁶⁵³ and Tyr⁶⁵⁴) and at the site of PLC binding (Tyr⁷⁶⁶), one residue in the juxtamembrane region (Tyr⁴⁶³), two in the kinase insert (Tyr⁵⁸³ and Tyr⁵⁸⁵), and one in the C-terminal lobe (Tyr⁷³⁰) were identified. Site-directed mutagenesis established that at least one of the A-loop tyrosine residues was necessary, but the mutation of the remainder of the sites did not affect FRS2 activation nor did it affect mitogenic signaling in L6 cells or neuronal differentiation on PC12 cells (8, 9, 16). In contrast, Wang and Goldfarb (17) found that the two tyrosine residues in the kinase insert were a necessary (but not sufficient) component of mitogenic signaling by FGFR1 in BaF3 lymphoid cells.

To further assess the specific and global contributions of the FGFR1 endodomain tyrosine residues to receptor function, these residues were systematically substituted with phenylalanine residues in a PDGFR/FGFR1 chimera and stably transfected into PC12 cells. Although none of the previously reported sites of phosphorylation outside of the A-loop were found to be required for neural differentiation, two residues adjacent to the A-loop (Tyr⁶⁷⁷ and Tyr⁷⁰¹) were required for induction of PC12 cell differentiation by the chimeric receptors. These residues may function to stabilize the A-loop in an active conformation following ligand binding.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Cloning of FGFR1-- A full-length cDNA clone (p31) encoding a 2-Ig loop form of FGFR1 was obtained from a PC12 cDNA library in  ZAP (Stratagene). The library was screened with a cDNA probe encompassing the extracellular domain of human FGFR1 (kindly provided by Dr. Michael Jaye). The clone was sequenced by the dideoxy method using Sequenase and by automated sequencing on an ABI sequencer.

Chimera Subcloning and Site-directed Mutagenesis-- As described previously (18), the naturally occurring 5' EcoRI and 3' MseI cDNA fragment of human PDGFR- encoding the N-terminal extracellular domain was ligated with a 5' MseI site introduced by polymerase chain reaction at the extracellular/transmembrane junction of the cDNA encloding the rFGFR1 intracellular domain and flanked by a 3' EcoRI site, also introduced by polymerase chain reaction. The construct, designated PFR1, was subcloned into the EcoRI site of pCMV for mutagenesis and transient expression in 293 cells. Stable transfection into PC12 cells was accomplished with a retroviral vector (see below). Tyrosine to phenylalanine mutations were generated using the CLONTECH Transformer site-directed mutagenesis kit. The substitutions were performed with only single base pair changes. All PFR1 cDNA constructs used in this study were sequenced to confirm that the site-directed mutagenesis was correct.

PC12 Cell Culture and Differentiation Assay-- Stable transfections were performed as described previously (18). Briefly, the viral packaging cell line GP+e86 was transfected with the pLEN-PFR1 constructs by the calcium phosphate method. After 2 days, media from retroviral producing GP+e86 cells was filtered (0.45 µm) and added to PC12 cells. After 24 h, PC12 cells were subjected to G418 (Life Technologies, Inc.) selection until individual colonies could be selected. PFR1 expressing PC12 cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 2.5% plasma derived fetal calf serum, 5% plasma-derived horse serum (Cocalico), and 1% penicillin/streptomycin (Life Technologies, Inc.). To quantitate the level of differentiation, PFR1 expressing PC12 cell lines were seeded at a density of 1 × 10⁵ cells/well on collagen (Collaborative Research)-coated 6-well tissue culture dishes (Falcon) in Dulbecco's modified Eagle's medium supplemented with 1% plasma-derived horse serum. Cells expressing PFR1 or mock-transfected cells were stimulated with 30 ng/ml PDGF-BB (Austral) or with medium alone as a control. The kinetics and extent of neurite outgrowth was measured as the percentage of cells with neurites greater than two cell bodies in length at specified times.

Immunoprecipitation and Immunoblot Analysis-- Protein was extracted from cells in lysis buffer (10 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% Triton X-100, 50 mM NaC1, 30 mM sodium pyrophosphate, 50 mM NaF, 1 mM phenylmethylsulfonyl fluoride, 100 µM Na₃VO₄) and quantitated. One mg of lysate was immunoprecipited with antibody prior to addition of protein A-Sepharose (Amersham Pharmacia Biotech) and washed three times in lysis buffer. The samples were then subjected to SDS-PAGE before electrotransfer to polyvinylidene difluoride membrane. For immunoblot analysis, the membranes were blocked at room temperature in Tris-buffered saline (TBS) containing 3% bovine serum albumin and blotted with primary antibodies. The membranes were washed in TBS and probed with the appropriated horseradish peroxidase conjugated secondary antibody (Amersham Pharmacia Biotech) diluted in TBS containing 5% nonfat dry milk. After three washes in TBS the bands were visualized by ECL chemiluminescent detection system (Amersham Pharmacia Biotech). Before the membranes were reprobed, they were stripped in 0.2 M glycine-HCl, pH 2.l5, 0.05% Tween 20 at 80 °C. The antibodies used for immunoprecipitation and immunoblot analysis include anti-FGFR1, PY20, anti-SOS, and anti-PLC, which were purchased from Santa Cruz Biotechnology; the anti-Grb2, anti-Shc, and p13suc-agarose was purchased from Transduction Laboratories. The anti-PDGFR antibody used for immunoprecipitation was purchased from Genzyme, and anti-PDGFR antibody used for immunoblot analysis was purchased from Austral.

In Vitro Kinase Assay-- Cells were incubated in eukaryotic lysis buffer and immunoprecipitated with anti-PDGFR (R & D Systems) and protein A-Sepharose (Amersham Pharmacia Biotech). The immune complexes were washed three times in lysis buffer and once in kinase buffer (20 mM HEPES, pH 7.4, 2 mM MnCl₂, 10 mM MgCl₂, 25 mM glycerol-2-phosphate, o.1 mM Na₃ VO₄, 4 mM NaF, 1 mM dithiothreitol, and 20 µM ATP), resuspended in 20 µl of kinase buffer, and incubated for 30 min at 30 °C with 5 µM [-³²P]ATP (6000 ci/mmol). The kinase reactions were terminated by the addition of SDS-PAGE sample buffer. The samples were analyzed by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and exposed to Hyperfilm (Amersham Pharmacia Biotech). The membranes were subsequently immunoblotted with anti-FGFR1 antibodies (Santa Cruz Biotechnology) to determine the relative amount of immunoprecipitated chimera.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Rat PC12 FGFR1 Clone-- The rat FGFR1 clone (p31) selected to prepare the chimeras used in this study was isolated from a PC12 cDNA library in  ZAP. This clone was characterized by a 2-Ig domain ectodomain and a Thr-Val insert in the juxtamembrane region. This motif has been shown to be a protein kinase C site that is important for the regulation of receptor activity in a splice variant from Xenopus embryos. Several clones judged to contain 3-Ig domain structures from size considerations were identified but not sequenced.² A comparison of the transmembrane and endodomains of the p31 sequence to those of two other rat FGFR1 clones revealed that they were identical to those of a rat kidney FGFR1 clone (GenBank^TM accession no. 554008) (except for the absence of the Val-Thr insert) but differed from a rat brain sequence (GenBank^TM accession no. D12498) at five sites (Table I). Two of these sites involve tyrosine residues (Tyr⁷⁰¹ and Tyr⁷⁷⁶). Thus, the brain isoform contains only 13 tyrosine residues in the endodomain as compared with the 15 found in the kidney and PC12 versions.

The tyrosine residues have been numbered in this report to correspond to those of human FGFR1 (GenBank^TM accession no. P11362) or the 3-Ig domain rat receptor). They do not correspond to the true sequential positions in the chimera (as the PDGFR ectodomain is larger than that of hFGFR1) or the 2-Ig loop sequence of p31. However, using the hFGFR1 numbers allows a direct comparison to several previous studies of FGFR1 and avoids the confusion of multiple numbering systems.

PFR1 Intracellular Domain Mutagenesis: Effects on Neurite Proliferation-- PFR1, constructed from the p31 clone, contains 15 tyrosines in the endodomain as illustrated at the top of Fig. 1. In studies with hFGFR1 (and a recombinant kinase domain derivative), Mohammadi et al. (15) identified six phosphorylated tyrosine residues (Tyr⁴⁶³, Tyr⁵⁸³, Tyr⁵⁸⁵, Tyr⁶⁵³, Tyr⁶⁵⁴, and Tyr⁷³⁰) in the activated receptor. Tyr⁷⁶⁶ was previously identified as a seventh site (8, 9). These sites are marked with an asterisk in Fig. 1.

View larger version (36K): [in this window] [in a new window]   Fig. 1.   Strategy for the mutagenesis of endodomain tyrosines of PFR1. The intracellular region of FGFR1 is depicted, and the 15-tyrosine residues are labeled. The 7 tyrosine residues previously identified as sites of phosphorylation (15) in vivo are marked with asterisks. The intracellular domain has been divided into four regions: A, B, C, and D. Mutant receptors are identified by the region and the number of residues changed to phenylalanine. Sequence numbering is for hFGFR1.

To evaluate the contributions of all of the endodomain tyrosine residues to the signaling potential of PFR1, and by extrapolation of FGFR1, they were converted to phenylalanine, a substitution normally expected to maintain hydrophobic integrity but lacking the opportunity for post-translational modification or H-bonding capacity. To facilitate these changes, the tyrosine residues were subdivided into four blocs, denoted A, B, C, and D (Fig. 1). Blocs A and B contained four tyrosine sites, and D, five sites. The C bloc comprised only the pair of tyrosines that are situated in the A-loop and whose combined phosphorylation stabilizes the active form of the kinase (5). The Tyr to Phe mutations were introduced en bloc and the resulting derivatives stably transfected into PC12 cells. These are designated by the bloc and number of residues modified, e.g. PFR1 A4 indicates all of the tyrosine residues of the A bloc have been converted to phenylalanine (Fig. 1).

The effect of the Tyr to Phe mutations in blocs A, B, C, and D, singly and in various combinations, on the biological response (neurite outgrowth) of the stably transfected cells is shown in Fig. 2A. All of the cells examined showed a moderate ligand-induced decrease in activity (after 48 h) as compared with unmodified PFR1, but the derivatives with modified A or B blocs (or both) eventually achieved full differentiation (data not shown). In contrast, all of the derivatives in which either the first four (D4) or all five (D5) tyrosines of the D bloc had been modified (D4, A4/D4, B4/D4, A4/B4/D4, and A4/B4/D5) were inactive. As expected, the C2 derivative, with all of the other 13 tyrosines of the endodomain intact, was also inactive. These results establish that none of the total of eight tyrosine residues in the N-terminal portion of the kinase domain (including the juxtamembrane sequence and kinase insert) are essential, although the derivatives lacking some or all of these residues are modestly less active than the native (PFR1) receptor (Fig. 2A). At the same time, the results indicate that one or more of the first four residues of the D bloc (Tyr⁶⁷⁷, Tyr⁷⁰¹, Tyr⁷³⁰, and Tyr⁷⁶⁶) is required.

View larger version (24K): [in this window] [in a new window]   Fig. 2.   PDGF-induced neurite formation of stably transfected PC12 cells bearing PFR1 Tyr  Phe mutants. The various PFR1 expressing PC12 cell lines were stimulated with 30 ng/ml PDGF in 6-well collagen-coated dishes and scored for the formation of neurites more than two cell bodies in length after 2 days. The percentage of cells bearing neurites is depicted and represents triplicate experiments (>200 cells/experiment; S.D. < 5%). Nomenclature is as described for Fig. 1. For the D bloc mutants (panel B), the position of each modified residue is indicated: 1 = Tyr677; 2 = Tyr701; 3 = Tyr730; 4 = Tyr766; 5 = Tyr776. Panel A, full bloc mutants; panel B, D bloc mutants: IF = 1 Phe; 2F = 2 Phe; 3F = 3 Phe.

To more specifically evaluate the contributions of the D bloc tyrosines, which clearly strongly affect neurite outgrowth activity, a total of 18 constructs (Fig. 3) with each site converted from Tyr to Phe in single, double, or triple combinations were prepared using the A4/B4 construct as a parent. These were stably transfected into PC12 cells and each derivative tested for neurite production by PDGF. The results of these assays are shown in Fig. 2B. The five tyrosines in the D bloc were numbered 1 to 5 (consecutively) for identification and correspond to Tyr⁶⁷⁷, Tyr⁷⁰¹, Tyr⁷³⁰, Tyr⁷⁶⁶, and Tyr⁷⁷⁶ (see Fig. 1). Thus, PFR1 A4/B4/D1-1 (designated simply D1-1 in Figs. 2B and 3) indicates the derivative in which the first tyrosine of the D bloc (Tyr⁶³⁰) has been converted to phenylalanine (along with the eight residues of blocs A and B).

View larger version (34K): [in this window] [in a new window]   Fig. 3.   Strategy for the mutagenesis of the tyrosines in block D of the endodomain of PFR1. All D bloc mutants depicted were made with the A4/B4 derivative (see Fig. 1). Nomenclature is as described for Figs. 1 and 2.

The individual substitution of each of the D bloc tyrosines (the D1 set marked 1F in Fig. 2B) has variable affects on the differentiative activity of the stably transfected chimeras after 48 h. The derivatives with substitutions at Tyr⁶⁷⁷ or Tyr⁷⁰¹ (D1-1 or D1-2, respectively) showed substantially decreased activity (~10-20% of control); the other three derivatives (Y730F, Y766F, and Y776F) showed activity of about 50% of control. Two of these latter residues (Tyr⁷³⁰ and Tyr⁷⁶⁶) are phosphorylated when FGFR1 is activated (15).

The pairwise substitutions of the D bloc tyrosines (the D2 set marked 2F in Fig. 2B) further supports the view that the substitution of the first two tyrosines (Tyr⁶⁷⁷ and Tyr⁷⁰¹) by phenylalanine is responsible for the loss of activity of the various D4 and D5 derivatives (Fig. 2A). The D2-12 derivative is inactive, whereas the D2-34 derivative is about as active as the chimeras with the single substitutions of these residues. The fifth tyrosine (Tyr⁷⁷⁶) does not make a significant contribution because the triple mutant (D3-345) in which this residue is also altered is almost as active as the D2-34 derivative (Fig. 2B). The particular importance of Tyr⁷⁰¹ is emphasized in the observations that every chimera with a double or triple substitution that includes the Y701F mutation is inactive. However, the derivative that contains only Tyr⁷⁰¹ (D3-134) is also inactive. (Although these derivatives also contain tyrosine D1-5 (Tyr⁷⁷⁶), this residue clearly makes no contribution; cf. D2-13 with D3-135 and D2-34 with D3-345, which have effectively the same activities (Fig. 2B)). The low but quite significant activity of D3-135 as compared with D3-134 suggests that tyrosine Tyr⁷⁶⁶, which has been shown to be the site of PLC activation (8, 9), may contribute somewhat to differentiation (presumably via PLC activation) when the level of FRS2 activation is lowered significantly (see below). This would also explain the comparable activation of D2-13 and D2-5 (with D3-135) and the very low activity of D2-4.

Effects on Receptor Autophosphorylation-- To correlate the cellular responses with receptor-associated molecular events, several of the stably transfected cell lines were examined with respect to receptor autophosphorylation following PDGF stimulation and by in vitro kinase assays. As shown in Fig. 4A and B, receptor autophosphorylation as measured by anti-phosphotyrosine immunoblots of anti-FGFR1-treated PC12 cell lysates closely paralleled the neurite proliferation activities (Fig. 2). Only the A4/B4/D2-23 derivative, which was inactive in the differentiation assay, showed significant autophosphorylation. Interestingly, the amount of modification observed was also generally comparable with the bioactivity even though these measurements are not strictly quantitative.

View larger version (49K): [in this window] [in a new window]   Fig. 4.   Autophosphorylation of PFR1 chimeric receptors. A and B, native PC12 cells or those stably expressing the indicated chimeras were incubated for 10 min in the presence or absence of 30 ng/ml PDGF. 1000 µg of cell lysate was incubated with FGFR1 antibody, collected with protein A-Sepharose, and subjected to 7.5% SDS-PAGE and immunoblot analysis (IB) with anti-phosphotyrosine antibody (PY, upper panels) and anti-PDGFR antibody (lower panels). C, 1000 µg of cell lysate was incubated with anti-PDGFR antibody, collected with protein A-Sepharose, and subjected to an in vitro kinase assay (IVKA, upper row) and anti-FGFR1 immunoblotting (lower row). Details of the assay are given in the text. IP, immunoprecipitate.

A more sensitive in vitro kinase assay was also used to examine the autophosphorylation of the A4/B4/D2-12 derivatives (Fig. 4C). As expected, the C2 chimera did not show any capacity for autophosphorylation in this test either. However, both the D4 and D2-12 derivatives were clearly weakly but significantly labeled. The similar level of modification suggests that it is the C2 tyrosines that are modified (rather than the D3 or D4 tyrosines that are absent in the A4/B4/D4 chimera). These findings also indicate that these (and probably all of the various D bloc mutants) are properly folded as they have low but detectable levels of catalytic activity.

Effects on the Activation of ERK, SOS, FRS2, and PLC-- The ability of the various PFR1 tyrosine derivatives to activate FGFR1-sensitive signaling pathways was assessed by immunoprecipitation and Western blotting of cell lysates following PDGF stimulation. The activation of ERK1/2 was determined using antibodies specific for the phosphorylated form of their MAPK. As shown in Fig. 5A, ERK1/2 is substantially activated by PDGF in PFR1 cells but not in cells bearing either the C2 or A4/B4/D4 variants. The A4, B4, and A4/B4 derivatives also activated ERK1/2 in a ligand-dependent manner in keeping with their differentiative activity. The D2-34 derivative showed weaker activation of ERK1/2, comparable with its weaker stimulation of neurite production, whereas D2-12 showed no ERK1/2 activation (Fig. 5B).

View larger version (60K): [in this window] [in a new window]   Fig. 5.   Tyrosine phosphorylation pattern of PC12 cells following stimulation of mutant chimeric receptors. Native PC12 cells or those expressing the PFR1 chimeras were incubated for 10 min in the presence or absence of 30 ng/ml PDGF. 100 µg of cell lysate was subjected to 7.5% SDS-PAGE, and immunoblot analysis (IB) was performed with anti-phosphotyrosine (PY), anti-PDGF, and anti-active ERK as indicated. Panels A and B represent different groups of chimeras tested.

FGFR1 as well as the other FGFRs (6) activate a scaffold protein FRS2 that interacts constitutively with FGFR1 (Fig. 6) (14). Upon phosphorylation, it binds Grb2 and Shp2, leading to the binding of SOS and activated RAS that subsequently causes ERK1/2 activation (10-13). As shown in Fig. 6, FRS2 activation of the various derivatized chimeras, as determined with anti-phosphotyrosine antibodies, is consistent with both the neurite proliferation and ERK1/2 activities of these receptors. Although clearly not quantitative, the levels of FRS2 phosphorylation closely parallel the differentiative activity. A similar profile was observed for SOS association with activated FRS2 (Fig. 7), although SOS association was not observed with the D1-1 and D1-2 derivatives (at the exposure shown).

View larger version (59K): [in this window] [in a new window]   Fig. 6.   Association of the mutant PFR1 chimeric receptors with FRS2. Native PC12 cells or those expressing chimeras were incubated for 10 min in the presence of 30 ng/ml PDGF. 1000 µg of cell lysate was incubated with FRS2 antibodies, collected with protein A-Sepharose, and subjected to 7.5% SDS-PAGE and immunoblot analysis (IB) with anti-FRS2 antibodies, anti-phosphotyrosine (PY), and anti-PDGFR antibody as indicated. IP, immunoprecipitate.

View larger version (24K): [in this window] [in a new window]   Fig. 7.   The co-immunoprecipitation of SOS with FRS2. Native PC12 cells or those expressing the chimeras were incubated with SOS antibodies, collected with protein A-Sepharose, and subjected to 7.5% SDS-PAGE and immunoblot analysis (IB) with anti-SOS antibodies and anti-phosphotyrosine antibody (PY). IP, immunoprecipitate.

PLC is specifically bound to tyrosine D4 (Tyr⁷⁶⁶) of FGFR1, and therefore derivatives lacking this residue would not be expected to show activation of this enzyme. Indeed, D4, A4/B4/D2-34, and A4/B4/D1-4 lack this capacity (Fig. 8), whereas the A4, B4, and A4/B4 derivatives all show PLC phosphorylation. Interestingly, the A4/B4/D2-12 chimera, which is inactive in neurite proliferation assays and ERK activation, also fails to activate PLC even though there is weak kinase activation (Fig. 4C), and the requisite tyrosine (Y766) is intact.

View larger version (31K): [in this window] [in a new window]   Fig. 8.   The activation of PLC by select PFR1 chimeric receptors. Native PC12 cells or those expressing the chimeras were incubated for 10 min in presence of 30 ng/ml PDGF. 1000 µg of cell lysate was incubated with PLC antibodies, collected with protein A-Sepharose, and subjected to 7.5% SDS-PAGE and immunoblot analysis (IB) with anti-PLC antibodies and anti-phosphotyrosine (PY) antibody. IP, immunoprecipitate.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

RTKs use a variety of strategies to recruit and activate signaling effectors but they share significant similarities in their mechanisms of auto-activation (1, 2). Generally, RTKs rely on the ligand-induced formation of phosphorylated tyrosines located in the juxtamembrane, kinase domain, or C-terminal extension to provide binding sites for PTB- or Src homology 2-containing effectors or adaptors (19). These sites can be highly selective for a particular signaling protein or be relatively promiscuous, binding several signaling entities. Thus, mutations of these residues (usually to phenylalanine) can cause specific changes in a receptor's signaling capacity or show little effect. At the same time, not all receptor tyrosine residues that are phosphorylated apparently act as docking sites, and it cannot be assumed that all endodomain tyrosines of a receptor that can be phosphorylated will necessarily be detected. By systematically eliminating all of the tyrosine residues in the endodomain, the role (or lack thereof) of each to the signaling capacity of a receptor can be assessed. The results reported herein indicate that there is a minimum of four tyrosines in rat FGFR1 required for function in PC12 cell differentiation: the two activation loop residues (Tyr⁶⁵³ and Tyr⁶⁵⁴) and two C-terminal lobe residues (Tyr⁶⁷⁷ and Tyr⁷⁰¹), which were not previously identified as either sites of modification or necessary for activity. Because Mohammadi et al. (15) previously showed that in FGFR1 Tyr⁶⁵³ could also be changed to phenylalanine while still retaining activity, there may only be three tyrosines (Tyr⁶⁵⁴, Tyr⁶⁷⁷, and Tyr₇₀₁) absolutely required for activity in these cells.

To some considerable degree, the contribution of the D1-1 and D1-2 tyrosines (Tyr⁶⁷⁷ and Tyr⁷⁰¹) to both receptor activation and downstream signaling can be elucidated from the data in Figs. 2 and 4. Autophosphorylation reflects both the state of the kinase activation and the number of receptor phosphorylation sites. Although these are not likely to be equally modified, one would nevertheless expect that derivatives in which modifiable tyrosines had been converted to phenylalanine would show lower amounts of phosphorylation. There are seven sites of tyrosine phosphorylation in FGFR1 (15), and the identical responses of FGFR1 and PFR1 in stably transfected PC12 cells suggest that the phosphorylation pattern of PFR1 will be comparable (18). The A4 and B4 blocks cover one and two sites, respectively, leaving only a total of four sites in these derivatives. The A4/B4 chimera accordingly would be expected to show lower levels of autophosphorylation for this reason alone. However, it is 25% less active in the neurite outgrowth assay at 48 h as compared with PFR1 (Fig. 2), suggesting some of the decreased autophosphorylation may reflect decreased kinase activity.

Several other comparisons confirm that the effect of Tyr⁶⁷⁷ and Tyr⁷⁰¹ on autophosphorylation reflects decreased kinase activity rather than just a decrease in available sites. A comparison of the A4/B4/D4 and A4/B4/D2-34 chimeras shows that the latter is well modified (comparable with the A4/B4 derivative), whereas the A4/B4/D4 modifications can be detected only with the in vitro kinase assay (Fig. 4C). Both contain only the C2 tyrosines as known modification sites (Tyr⁷³⁰ and Tyr⁷⁶⁶). Clearly these latter two residues are not modified when the D1 and D2 tyrosines are substituted, which is consistent with the lack of activation of PLC by the latter derivative (Fig. 8). It is also instructive to compare the A4/B4 derivative with the A4/B4/D1-1 and D1-2 mutants. These chimeras all contain four potential tyrosine autophosphorylation sites, but the latter are clearly modified to a lesser degree and have substantially reduced neurite proliferation activity.

Tyrosines 677 and 701 are not believed to be sites of autophosphorylation (15), which is consistent with their relatively buried positions as determined in structural analyses of the FGFR1 kinase domain (marked in blue in Fig. 9) (5). Thus, these tyrosines are likely important structurally, but when mutated to phenylalanine, they do not appear to cause misfolding or instability of the receptor. Indeed, derivatives involving these residues retain measurable catalytic activity, albeit quite low in some cases, indicating that correct folding has occurred. However, the presence of an aromatic ring is apparently insufficient to meet their roles in the fully active kinase, and a H-bonding capacity is also required either structurally or to play a more direct role in the activation process. A comparison of the FGFR1 structure with the unliganded IRK structures leads to the following conclusions (4, 5, 20). Tyr⁶⁷⁷ lies closest to the A-loop and, although not showing any interaction in the FGFR1 structure, the environment of the equivalent Phe¹¹⁸⁶ changes drastically between the two forms of the IRK. In the activated form, it lies beneath the A-loop, and a tyrosine here would have the potential to H-bond to and stabilize the loop in the activated form. In the FGFR1 structure, Tyr⁷⁰¹ is partially buried in a group of mainly hydrophobic residues and forms a H-bond with the side chain of His⁷¹⁷. In the IRK structures this region remains essentially unchanged between the two forms; the equivalent residue (Tyr¹²¹⁰) makes no contacts with side chains but is involved in water-mediated H-bonding to the carbonyl groups of 1174 and 1176 and the main chain nitrogen of 1228, thus bridging secondary structure elements. Tyr⁷⁰¹ lies on an extended loop between -helices F and G, which follow a similar trace in all three structures. If the A-loop adopts a similar conformation in fully activated FGFR to that in IRK, a readjustment of the G loop would be required for Tyr⁷⁰¹ to make a stabilizing contact. Such a feature is not, however, seen in the two known tyrosine kinase structures with activated conformation A-loop, i.e. IRK and the non-receptor Lck kinase (21).

View larger version (39K): [in this window] [in a new window]   Fig. 9.   Representation of the kinase domain of FGFR1. -Helices are shown in gray-green, and -sheets are indicated by blue arrows. Tyrosine residue side chains are shown with the following colors: sites of phosphorylation are in red (Tyr583 and Tyr585 are not defined), A-loop tyrosines are in magenta, the three tyrosines that are unmodified are in gray, and two that are required for activity (this study) are in blue. Numbering is from hFGFR1 (GenBankTM accession no. P11362).

Tyr⁶⁷⁷ and Tyr⁷⁰¹ are well conserved in the FGFR family. Only Halocynthia noretzi, an invertebrate worm, has a Phe to Tyr substitution at residue 677. Interestingly, this same sequence has a H717E substitution, although Tyr⁷⁰¹ is conserved. The only substitution of Tyr⁷⁰¹ is the Asn replacement found in the rat brain form of FGFR1 (see Table I). The significance of this substitution is unclear, but this residue does retain H-bonding capacity, although it is somewhat shorter in length than the tyrosine side chain.

These same residues are widely but not universally conserved in other tyrosine kinases including those associated with receptor structure. Tyr⁶⁷⁷ (of hFGFR1) is also found in PDGFR, CSFIR, EGFR, VEGFR, and Ret but is not found in IR, Met, or TrkA. It is also found in some of the smaller tyrosine kinases such as CDK2 and Erk2 but not in Src, Lck, or Abl1. Tyr⁷⁰¹, on the other hand, is present in a broad range of RTKs and non-RTKs. Of the above mentioned enzymes, it is substituted only by phenylalanine in CDK2 and Erk2. In TrkA, this position is actually occupied by a tryptophan residue followed by a tyrosine, and some repacking of this region would be expected. It's H-bonding partner, His⁷¹⁷ in FGFR1, is not conserved between kinases, and there are probably subtle ways in which Tyr⁷⁰¹ acts within the different receptors.

The studies described in this report reflect the structural requirements in one cell type. Furthermore, as they were carried out in situ, they represent the activation/deactivation processes of a living cell. Although the connection between ligand binding and kinase activation is incompletely understood, it is clear that this activation normally requires a dimeric structure. Either as the result of dimerization or as the subsequent reorientation of the protomers in a preformed dimer, ligand binding leads to a conformational change in the kinase domain that is characterized by A-loop movement and changes in orientation/conformation of the two principal lobes forming the active site cleft. The former is often accompanied by phosphorylation events that further stabilize this conformation, presumably by forming sites of interaction between phosphate groups and basic (lysine or arginine side chains) centers. The substitution of a glutamic acid for Lys⁶⁵⁰ in FGFR3 (the basis for the human disease thanatophoric dysplasia II) (22), two residues downstream from the tyrosine residues corresponding to Tyr⁶⁵³ and Tyr⁶⁵⁴, leads to autoactivation by providing an alternative interaction of this sort that is not reversible.³ In a cellular milieu, the phosphorylation of the activation loop tyrosines is balanced by dephosphorylation events. The availability of these modified residues as substrates will thus depend on the degree to which the modified loop is stabilized in the open conformation. If Tyr⁶⁷⁷ and Tyr⁷⁰¹ contribute directly or indirectly to this structure, their absence would likely accelerate the reverse reactions. Importantly, it cannot be stated with certainty that these derivatives do not play purely a structural role and that the kinase domains of these inactive derivatives (lacking Tyr⁶⁷⁷ and/or Tyr⁷⁰¹) are simply inactive. However, the strong autophosphorylation of the A4/B4/D2-23 derivative, which lacked measurable differentiative activity, argues against this as a general condition.

The identified sites of autophosphorylation are shown in Fig. 9 (in red). Tyr⁷⁷⁶ (the PLC site) lies outside the domain pictured, and the kinase insert residues (Tyr⁵⁸³ and Tyr⁵⁸⁵) were not defined because of poor resolution in this part of the structural analysis (5). Mohammadi et al. (15) determined that the elimination (by mutation to phenylalanine) of the modified tyrosines was without effect on L6 or PC12 cell responses (with the exception of Tyr⁶⁵³ and Tyr⁶⁵⁴ as already noted). However, Wang and Goldfarb (17), using chimeras of FGFR1 and FGFR4, found that the two tyrosines in the kinase domain insert (Tyr⁵⁸³ and Tyr⁵⁸⁵) were required for full mitogenic activity in BaF cells. It has been shown that previously Tyr⁷⁷⁶ could be altered without affecting L6 and PC12 cell responses, indicating that PLC was unnecessary in these cases (8, 9, 16). However, PLC can contribute to FGFR1 function, as has been demonstrated in neuronal growth paradigms (23) and herein. PLC can make a contribution, at least to PC12 differentiation, if the receptor lacks the capacity for the full activation of other pathways. This can be seen clearly in the comparison of the activity of A4/B4 and A4/B4/D1-4, with the latter lacking the PLC activation site. The lower but significant activity of A4/B4/D2-13 and D2-15 most likely also results from a PLC contribution that is absent, for instance, from the D2-14 derivative. Indeed, TrkA derivatives in PC12 cells that lack the activation site for FRS2 (or Shc) do show ligand-induced neurite outgrowth, but at greatly reduced levels, that is generated solely by PLC activation (23).

The elimination of the nonphosphorylated tyrosines (as well as the modified ones) also establishes that this receptor does not contain cryptic (or undetected) additional residues of phosphotyrosine, which is consistent with the view that the FGFRs signal solely through FRS2 and PLC activation. The former associates constitutively with FGFRs (13), as was also observed in these studies, and is independent of the state of activation. The binding site has been identified as a 12-residue sequence in the juxtamembrane domain, which is devoid of tyrosine residues (14). Whether activation of the FGFR allows for tighter binding and therefore enhanced enzyme-substrate interactions is unknown, but the elimination of the tyrosines, particularly in the A4/B4 blocks that are closest to the site of interaction, has only modest effects on the differentiation capacity (See Fig. 2). This may reflect some alteration in FRS2-FGFR1 binding and the level of activation of the signaling scaffold, or it may simply indicate that the A4/B4 derivative is not as active (because it cannot stabilize the active form as effectively).

	FOOTNOTES

^* This work was supported by National Institutes of Health Research Grant AG09735.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Physiology and Biophysics, University of California, Irvine, CA 92697. Tel.: 949-824-6236; Fax: 949-824-8036; E-mail: rablab@uci.edu.

Published, JBC Papers in Press, July 17, 2001, DOI 10.1074/jbc.M103234200

² S. Raffioni, unpublished data.

³ D. Tyson, L. M. Thompson, and R. A. Bradshaw, manuscript in preparation.

	ABBREVIATIONS

The abbreviations used are: RTK, receptor tyrosine kinase; EGFR, epidermal growth factor receptor; PDGFR, platelet-derived growth factor receptor; A-loop, activation loop; IR, insulin receptor; IRK, insulin receptor kinase; FGFR, fibroblast growth factor receptor; PLC, phospholipase C; ERK1/2, extracellular receptor-stimulated kinase 1/2; FRS2, FGF receptor substrate 2; PFR1, PDGFR/FGFR1 chimeric receptor; MAPK, mitogen-activated protein kinase; PAGE, polyacrylamide gel electrophoresis; TBS, Tris-buffered saline.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Lonic, E. F. Barry, C. Quach, B. Kobe, N. Saunders, and M. A. Guthridge Fibroblast Growth Factor Receptor 2 Phosphorylation on Serine 779 Couples to 14-3-3 and Regulates Cell Survival and Proliferation Mol. Cell. Biol., May 15, 2008; 28(10): 3372 - 3385. [Abstract] [Full Text] [PDF]

				C. Schalk-Hihi, H.-C. Ma, G. T. Struble, S. Bayoumy, R. Williams, E. Devine, I. P. Petrounia, T. Mezzasalma, L. Zeng, C. Schubert, et al. Protein Engineering of the Colony-stimulating Factor-1 Receptor Kinase Domain for Structural Studies J. Biol. Chem., February 9, 2007; 282(6): 4085 - 4093. [Abstract] [Full Text] [PDF]

				Y. Chen, Y.-H. Li, X.-P. Chen, L.-M. Gong, S.-P. Zhang, Z.-J. Chang, X.-F. Zhang, X.-Y. Fu, and L. Liu Point Mutation at Single Tyrosine Residue of Novel Oncogene NOK Abrogates Tumorigenesis in Nude Mice Cancer Res., December 1, 2005; 65(23): 10838 - 10846. [Abstract] [Full Text] [PDF]

				M. J. Cross, L. Lu, P. Magnusson, D. Nyqvist, K. Holmqvist, M. Welsh, and L. Claesson-Welsh The Shb Adaptor Protein Binds to Tyrosine 766 in the FGFR-1 and Regulates the Ras/MEK/MAPK Pathway via FRS2 Phosphorylation in Endothelial Cells Mol. Biol. Cell, August 1, 2002; 13(8): 2881 - 2893. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 276/40/37529    most recent M103234200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foehr, E. D. Articles by Bradshaw, R. A. Search for Related Content PubMed PubMed Citation Articles by Foehr, E. D.