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J. Biol. Chem., Vol. 277, Issue 23, 20499-20503, June 7, 2002

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Separate Analysis of Twin-arginine Translocation (Tat)-specific Membrane Binding and Translocation in Escherichia coli^*

Meriem Alami, Dorothea Trescher, Long-Fei Wu^§, and Matthias Müller^¶

From the  Institut für Biochemie und Molekularbiologie, Universität Freiburg, Hermann-Herderstrasse 7, D-79104 Freiburg, Germany and ^§ Laboratoire de Chimie Bacteriénne, UPR9043 CNRS, Institut de Biologie Structurale et Microbiologie, 31 chemin Joseph Aiguier, F-13402 Marseille Cedex 20, France

Received for publication, February 20, 2002, and in revised form, March 19, 2002

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

The twin-arginine translocation (Tat) pathway exports those precursor proteins to the periplasmic space of bacteria that harbor a twin-arginine (RR) consensus motif in their signal sequences. We have reproduced translocation of several Tat substrates into inside-out plasma membrane vesicles from Escherichia coli. Translocation proceeding at an efficiency of up to 20% occurs specifically via the Tat pathway as indicated by (i) its requirement for elevated levels of the TatABC proteins in the membrane vesicles, (ii) competition by an intact twin-arginine signal peptide, and (iii) susceptibility toward dissipation of the transmembrane H⁺ gradient. The latter treatment, while blocking translocation, still allows for functional membrane association of Tat precursors. This is shown by the finding that translocation of isolated membrane-bound Tat precursor is restored upon re-energization of the vesicles.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Bacteria export proteins from their cytoplasm to the cell envelope, which consists of the cytoplasmic membrane, the surrounding periplasmic space and, in the case of Gram-negative organisms, also the outer membrane. Whereas the majority of these proteins are exported via the classical Sec pathway (1), several periplasmic proteins use the more recently discovered twin-arginine translocation (Tat)¹ pathway, a homologue of which is also operative at the thylakoidal membrane of chloroplasts (recently reviewed in Refs. 2-4). Substrates of the Tat pathway are distinguished by a highly conserved (S/T)RRXFLK motif in their signal sequences.

The most striking difference between the Sec and the Tat pathway of bacteria is the capability of the latter to transport fully folded proteins. As such, most Tat substrates have redox cofactors bound or even form oligomeric complexes prior to export (5, 6). This feature in combination with the fact that an RR signal can transport heterologous proteins (7, 8) renders the bacterial Tat pathway a potentially attractive biotechnological tool.

As of now, almost no details are known on how Tat substrates are targeted to and translocated by the cognate translocase. Both in thylakoids and in bacteria, a transmembrane H⁺ gradient is essential (5, 9). By deletion analysis, the membrane-embedded (10) TatA, TatB, and TatC proteins, which are conserved between bacteria and chloroplasts, were shown to be constituents of the Tat pathway (for references, see the indicated reviews). The Tat translocase of Escherichia coli appears to be represented by ~600-kDa complexes that, depending on the detergent used for solubilization, contain varying amounts of TatA, TatB, and TatC (11-13). In addition, one RR signal sequence-binding protein, unrelated to TatABC, has been described (14). By use of a cell-free system with unprecedented high efficiency, we show here that in E. coli, translocation of Tat substrates across the cytoplasmic membrane follows a targeting step that is independent of the H⁺ gradient but requires an intact RR signal.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Plasmids-- The pre-SufI and pre-SufI-R5K,R6K (SufI-KK) coding regions, subcloned from derivatives of pNR14 (15) into pBluescript SK (Stratagene) using the EcoRI-XbaI sites of the polylinker, were kindly provided by Dr. Tracy Palmer (John Innes Centre, Norwich, UK). The TorA-P2 fusion (16) cloned into pBAD18 was kindly provided by Drs. Jan Willem de Gier and Gunnar von Heijne (Stockholm University, Sweden). The TorA-P2 coding sequence was amplified by PCR using Pwo DNA polymerase (Roche Molecular Biochemicals) and 5'-GCTCAAGAAGGATCCAAAATAATGAAC-3' and 5'-CGACCCGGGCTATTAATGGATGCC-3' as forward and reverse primers, respectively. The amplified fragment was digested with BamH1 and SmaI and subcloned into the pKSM717 polylinker (17) to give plasmid p717TorP2. The inserted DNA was verified by sequencing. Plasmids pMW18 encoding the fusion protein TorA-23K (18) and pDMB encoding pre-OmpA (19) have been described.

The tatABCD gene cluster was amplified from the chromosome of E. coli strain TG1 by PCR using the primers 5'-GGCAGGTGGTCTGATCGTCTAGATTGTCGG-3' and 5'-TTTCACCGATCTAGATGGTGAGGCTCGCTC-3' with the Expand High Fidelity PCR System according to the manufacturer's instructions (Roche Molecular Biochemicals). The amplified 2621-bp fragment, which encompasses the entire tat operon and about 126 bp upstream of tatA and 95 bp downstream of tatD, was digested with XbaI and cloned into the same site of plasmid pET22b(+) (Novagen) behind the phage T7 promoter. The structure of the resulting plasmid p8737 was verified by EcoRV, HindIII, BstXI, and XmnI endonuclease digestion. Introduction of the plasmid p8737 into the mutants MCMTA (tatB::Kn) (20) and B1LK0 (tatC) (18) restored the growth of these strains on trimethylamine N-oxide under anaerobic conditions.

In Vitro Transcription/Translation-- An S-135 was prepared according to Ref. 21 from E. coli strain SL119 (BL21; recD::Tn10, F, hsdS, gal, OmpT) kindly provided by Dr. Richard Burgess (University of Wisconsin, Madison, WI). Cells were grown in a medium containing 9 g of bactotryptone, 0.8 g of yeast extract, 5.6 g of NaCl, 0.8 g of glucose, and 1 ml of 1 M NaOH/liter. After growth, cells were resuspended at 1 g/ml in 10 mM triethanolamine acetate (TeaOAc), pH 8, 14 mM Mg(OAc)₂, 60 mM KOAc, and 1 mM dithiothreitol (DTT) and passed twice at 8.000 p.s.i. through a French pressure cell. An S-30 was obtained by centrifugation of the homogenate for 30 min at 30,000 × g. 750-µl aliquots of membrane-free S-135 were obtained from 1-ml aliquots of S-30 by centrifugation at 90,000 rpm for 13 min at 4 °C in a TLA 100.2 rotor (Beckman).

Coupled transcription/translation from plasmid DNA was performed similarly as described in Ref. 21. The reaction mixture (25 µl) contained 40 mM TeaOAc, pH 7.5, 70 mM KOAc, 9 mM Mg(OAc)₂, 0.8 mM spermidine, 3.2% (w/v) polyethylene glycol 6000-8000, 2 mM DTT, 2.5 mM ATP, 0.5 mM each of GTP, UTP, and CTP, 10 mM KOH to neutralize the NTP, 8 mM phosphoenolpyruvate, 8 mM putrescine, 8 mM creatine phosphate, 40 µg/ml creatine phosphokinase, 40 µM each of 18 amino acids, 10 µCi of ³⁵S-labeled methionine and cysteine, 3-4 µl of S-135, 1 µg of plasmid DNA, 10 µg/ml rifampicin to inhibit the E. coli RNA polymerase, and 3 units of T7 RNA polymerase. Incubation was for 45 min at 37 °C. Where appropriate, inside-out inner membrane vesicles (INV) were added 5 min after the start of the incubation to a final concentration of 1-2 A₂₈₀ units/ml. Reactions were stopped as detailed in the legend of Fig. 1.

Preparation of Membrane Vesicles-- INV were prepared from E. coli strains MC4100 (F, lacU169, araD139, rpsL150, relA1, ptsF, rbsR, flbB5301 (22)) and DADE (MC4100, tatABCDtatE) (23). A crude membrane pellet obtained as described (21) was loaded on a three-step sucrose gradient (12 ml of 0.77 M sucrose, 12 ml of 1.44 M sucrose, and 10 ml of 2.2 M sucrose in 50 mM TeaOAc, pH 7.5, 1 mM EDTA, 1 mM DTT) and centrifuged for 16 h at 25,000 rpm at 4 °C in a SW27 rotor (Beckman). INV were withdrawn from the 0.77/1.44 M sucrose interface, pelleted after dilution, and resuspended in INV buffer (50 mM TeaOAc, pH 7.5, 250 mM sucrose, 1 mM DTT). Tat⁺-INV were prepared from E. coli strain BL21(DE3)pLysS (24) transformed with the plasmid p8737. The expression of the tat gene products was induced by the addition of 1 mM isopropyl thio--D-galactoside.

Low Salt Treatment of Membrane Vesicles-- The purified INV were stripped of their F₁ component of the F₁F₀-ATPase by low salt treatment (25). INV were pelleted in an Airfuge (Beckman) at 30 p.s.i. for 10 min through a sucrose cushion (500 mM sucrose, 50 mM TeaOAc, pH 7.5, 1 mM DTT). The pellet was resuspended in half of the original volume of low salt buffer (1 mM TeaOAc, pH 7.5, 250 mM sucrose, 0.5 mM EDTA, 1 mM DTT) and incubated on ice for 15 min. The low salt-extracted vesicles were again pelleted through a sucrose cushion (1 mM TeaOAc, pH 7.5, 500 mM sucrose, 0.5 mM EDTA, 1 mM DTT) in the Airfuge at 30 p.s.i. for 10 min. The supernatant above the sucrose cushion was collected as the low salt extract (LSE), and the pellet that consists of the washed vesicles (wTat⁺) was washed one more time in low salt buffer and resuspended in the original volume of INV buffer.

Miscellaneous-- Flotation centrifugation was performed as described (26). Polyclonal anti-TatB and anti-TatC antibodies were raised in rabbits using the peptides NH₂-CTTVQNELTQELKLQ EFQDSLKKVEK-COOH and NH₂-CGKGRNREEENDAEAESEKTEE-COOH, respectively. Peptides corresponding to the signal sequence of SufI (SLSRRQFIQASGIALAAGAVPLKASA) and SufI-KK (SLSKKQFIQASGIALAAGAVPLKASA) were a generous gift of Dr. Ben Berks (University of Oxford, Oxford, UK). The Cys at position 16 of the SufI signal peptide had been replaced by Ala.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

In Vitro Reconstruction of RR-dependent Translocation-- When sufI, encoding a cofactor-less Tat substrate of E. coli (15), was expressed from a T7 promoter in an E. coli transcription/translation system, one major translation product of about 52 kDa was obtained that corresponds in size to pre-SufI (Fig. 1A, lane 1). The presence during synthesis of INV of wild-type E. coli did not yield proteolytic processing of pre-SufI (lane 3) nor resistance to proteinase K (lane 4, PK). This indicated that, in contrast to the Sec substrate OmpA (Fig. 1B), removal of the signal peptide and translocation of pre-SufI into the lumen of wild-type vesicles had not occurred. Similar to a previous finding (9), proteolytic processing and protease resistance of pre-SufI were, however, observed when INV had been prepared from a TatABCD-overproducing strain (Fig. 1A, lanes 5 and 6, Tat⁺-INV). As expected, these Tat⁺-INV did not exhibit any significant change in translocation of the Sec-dependent OmpA when compared with wild-type INV (not shown). Because proteolytic processing and protease resistance of pre-SufI were completely abolished by the dissipation of the H⁺ gradient of the Tat⁺-INV using the uncoupler carbonyl cyanide m-chlorophenyl-hydrazone (CCCP) at 100 µM (lanes 7 and 8), these phenomena cannot be due to unspecific cleavage or inherent protease resistance but rather reflect true translocation of pre-SufI into Tat⁺-INV. This was further confirmed by the abolishment of protease resistance in the presence of a membrane-disrupting detergent (not shown). Fig. 1C illustrates the overproduced amounts of TatB and TatC in Tat⁺-INV, and similar results were obtained for TatA (not shown).

View larger version (33K): [in this window] [in a new window]   Fig. 1.   Efficient H+-dependent translocation of several Tat-precursors into INV. A and B, the indicated proteins were synthesized by a coupled transcription/translation system prepared from E. coli. [35S]methionine/cysteine-labeled translation products were separated by SDS-PAGE and visualized by phosphorimaging. Where indicated, INV from wild-type strain (wt) or a TatABCD overproducer (Tat+) were present during synthesis. CCCP was added by 1:25 dilution from a 2.5 mM stock solution prepared in Me2SO. After synthesis, samples were divided in half, one half being directly precipitated with 1 volume of 10% trichloroacetic acid and the other half being precipitated only after 20 min of incubation at 25 °C with 1 volume of 1 mg/ml proteinase K (PK). The percentage of translocation was calculated by dividing the intensity of the proteinase K-resistant band by the sum of those of the precursor and mature form (open and closed arrowheads, respectively) and correcting for losses of Met/Cys due to the removal of the signal sequence. The asterisks mark uncharacterized cleavage products by wild-type INV of the TorA hybrids (see "Results"). C, immunoblot analysis of INV washed with 1 M KOAc (K-INV, 50 µg of protein/lane) prepared from the TatABCDE deletion mutant, a wild-type strain, and the TatABCD overproducer using anti-TatB and anti-TatC antibodies.

In three independent experiments, the extent of translocation of SufI into Tat⁺-INV was determined to be 20.5% ± 3.2. A similar value (18.4% ± 5.1) was found for another Tat substrate, TorA-23K (Fig. 1A), a hybrid protein consisting of the RR signal sequence of E. coli trimethylamine N-oxide reductase (TorA) and the 23-kDa subunit of the photosystem II oxygen-evolving complex from spinach chloroplasts (27). The 23-kDa protein is a substrate of the thylakoidal Tat apparatus. Another hybrid made of the TorA signal sequence and the otherwise Sec-dependent periplasmic P2 domain of E. coli leader (signal) peptidase (TorA-P2) (16) was also translocated into Tat⁺-INV albeit less efficiently (Fig. 1A; 5.1% ± 1.9).

In contrast to SufI, the two TorA hybrid precursors were proteolytically processed also by wild-type INV (lane 3, asterisks). These cleavage products, however, were not translocated into INV as they remained totally proteinase K-sensitive (compare lanes 4 and 6) and appeared even in the presence of CCCP (panel B), which otherwise effectively inhibited both the Tat (panel A, lane 7) and the Sec pathway (panel B, OmpA). Non-Tat-related processing appears to be a peculiarity of TorA hybrid proteins (16).

To confirm that the three Tat precursors were in fact translocated into the vesicles via the Tat pathway, competition experiments were performed (Fig. 2) using the signal sequence peptides of SufI (RR^signal) and a mutant peptide in which the two invariant arginines had been replaced by lysines (KK^signal). Translocation into Tat⁺-INV of all three Tat precursors was completely inhibited by the wild-type SufI signal peptide (Fig. 2A, lane 4). Inhibition was specific since it was not obtained with the mutant KK peptide (lane 6) nor was it observed for the Sec-dependent OmpA. Thus translocation of Tat substrates into Tat⁺-INV involves specific recognition of the RR signal sequence. Accordingly, the KK mutant precursor of SufI was not translocated into Tat⁺-INV (Fig. 2B).

View larger version (52K): [in this window] [in a new window]   Fig. 2.   In vitro translocation of Tat precursors requires an intact twin-arginine signal sequence. A, the indicated precursor proteins were synthesized in vitro for 45 min. Subsequently, samples were made 0.4 mM in puromycin using a neutralized stock solution of 11 mM, incubated for 5 min at 37 °C and then for another 45 min at 37 °C in the presence of Tat+-INV with buffer, K+ and Mg2+ salts, ATP, DTT, creatine phosphate, and creatine phosphokinase added to give the same concentrations as during the initial synthesis reaction. RRsignal, signal peptide of SufI; KKsignal, same but with the two consensus arginines exchanged against lysines. Peptides dissolved in H2O were added at 400 µM together with INV. PK, proteinase K. B, the precursor of the KK mutant of SufI was synthesized in vitro as described in the legend of Fig. 1.

Separating Functional Membrane Binding from Translocation-- To analyze the membrane targeting of Tat substrates, we determined the amount of INV-bound precursor by flotation centrifugation. Under the conditions employed (Fig. 3A), INV float predominantly to fraction 2 of the sucrose gradient and to some degree also to fraction 3, whereas non-membrane-associated material is recovered mostly from the pellet P. This is exemplified in the upper panel of Fig. 3A for OmpA, which, when synthesized in the absence of INV, largely stays in the pellet P. On the contrary, the majority of precursor and mature form of OmpA obtained in the presence of Tat⁺-INV is found in fraction 2. Likewise, in vitro-synthesized SufI and TorA-23K quantitatively floated with Tat⁺-INV (Fig. 3A). In contrast to OmpA, the Tat precursors did, however, not exhibit any significant flotation with wild-type INV (not shown) consistent with the low level of TatABC proteins of these vesicles being limiting for translocation.

View larger version (61K): [in this window] [in a new window]   Fig. 3.   Membrane targeting of Tat substrates precedes transmembrane translocation. A, precursors were synthesized in vitro in the presence of the indicated INV. wTat+, low salt-washed INV. Samples were subjected to flotation gradient centrifugation. The four fractions sequentially withdrawn from the top of the gradient were precipitated with trichloroacetic acid and together with the pelleted material (P), which was directly dissolved in SDS-PAGE sample buffer, analyzed by SDS-PAGE and phosphorimaging. The intensities of the bands corresponding to precursor and mature forms of OmpA, SufI, and TorA-23K were quantified with the sum of the five fractions each set at 100%. The given numbers are the means of two independent experiments, only one of which is shown. Note that in contrast to the two Tat precursors, signal sequence cleavage of OmpA is only partially sensitive toward low salt-stripping of INV (cf. panel A, fractions 2), reflecting the H+ gradient-independent initial stage of translocation of this Sec precursor (30). B, SufI and TorA-P2 were synthesized in vitro. Their translocation into the indicated INV was tested by proteinase K (PK) protection. C, SufI was synthesized in the presence of wTat+-INV. Lanes 3 and 4, samples were made 0.4 mM in puromycin, incubated for 5 min at 37 °C and then for another 45 min at 37 °C in the presence of LSE and the additions described in the legend for Fig. 2 (LSEpost). Lanes 5-8, SufI translation products derived from 800 µl of puromycin-treated reaction mix were separated by flotation centrifugation on 20 gradients; the combined fractions 2 containing membrane-targeted SufI (SufI-wTat+) were divided in half and incubated as described for lanes 3 and 4 in the absence or presence of LSE.

To analyze membrane binding independently of transmembrane translocation, we employed de-energized INV. Removal of the F₁-ATPase from INV by low salt stripping impairs translocation of Sec precursors by the dissipation of the H⁺-motive force and is reversed by the readdition of F₁-ATPase or the low salt extract (LSE) (28). Likewise (Fig. 3B), when Tat⁺-INV were treated with low salt (wTat⁺-INV), processing and translocation of SufI and TorA-P2 were completely abolished (compare lanes 3 and 4 with lanes 5 and 6). The low salt-stripped vesicles, however, regained RR translocation activity by the addition of the LSE (lanes 7 and 8).

Next, the flotation behavior of Tat precursors in the presence of wTat⁺-INV was analyzed (Fig. 3A). As found for Tat⁺-INV, the majority of SufI and TorA-23K floated with the low salt-extracted vesicles (cf. fractions 2 of the Tat⁺ and wTat⁺ gradients). The only difference between the two vesicle populations was the lack of signal sequence cleavage by wTat⁺-INV that, however, was restored by the low salt extract. Evidently, inactivation of the Tat machinery by the dissipation of the H⁺ gradient does not prevent the precursor from binding to the vesicles.

Translocation via the Tat Translocase Follows RR Signal Sequence-dependent Membrane Binding-- If the Tat precursors floating with low salt-stripped INV reflect populations of functional transport intermediates, translocation of the accumulated precursors should proceed once the H⁺ gradient has been restored. Pre-SufI was therefore synthesized in the presence of low salt-stripped Tat⁺-INV to allow for membrane targeting. Subsequently, the low salt extract was added (Fig. 3C, LSE^post), either directly or after isolating pre-SufI bound to low salt-stripped INV by flotation centrifugation (SufI-wTat⁺). In both cases, the low salt extract did restore processing and translocation of SufI (lanes 3, 4, 7, and 8), clearly indicating that the precursor had been targeted to the translocation-deficient vesicles in a functional manner.

To find out whether functional targeting requires an intact RR signal sequence, we investigated the flotation behavior of the translocation-defective SufI-KK. As summarized in Table I, the sedimentation pattern of pre-SufI-KK obtained after synthesis in the absence of INV hardly changed upon the addition of Tat⁺-INV with only a minor part of the pre-SufI-KK being recovered from the prime vesicle fraction 2. This was in clear contrast to the results observed with translocation-proficient precursors (cf. Fig. 3A). Moreover, sedimentation of pre-SufI-KK was only marginally influenced by de-energizing Tat⁺-INV with CCCP or by low salt stripping. These results therefore suggest that a specific targeting of Tat precursors requires an intact RR signal sequence.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

We have separated membrane targeting of bacterial twin-arginine preproteins from their translocation into inside-out plasma membrane vesicles. The cell-free system employed here does not grossly differ from previous ones described for the Sec-dependent protein export except that the usage of membrane vesicles prepared from a TatABCD overproducer was imperative. Although wild-type vesicles allow for efficient Sec-dependent translocation, they lack RR translocase activity for reasons that are not yet evident. However, the TatABC proteins have also been shown to be limiting for the Tat pathway in vivo (9, 13).

The efficiency of our in vitro system (up to 20% for at least two different Tat substrates) is by far higher than that of a similar one (0.4% for pre-SufI only) that was recently described (9) in which INV were routinely added after synthesis of pre-SufI. We have repeatedly made the observation that the later the vesicles are added after starting synthesis of the Tat precursors, the lower the efficiency of transport into these vesicles. However, why a precursor addressed to a dedicated posttranslational pathway should become translocation-incompetent in time remains unanswered thus far. In any event, a highly efficient in vitro system was a prerequisite to analyze functional precursor binding and translocation in a separate and stepwise manner by low salt stripping of INV.

Efficient membrane binding of RR precursors was observed only with INV harboring elevated levels of TatABC. The lack of Tat-specific translocation into wild-type INV seems to be correlated with an impaired targeting of Tat substrates to these vesicles, suggesting that TatABC proteins function as a receptor site for Tat-specific precursor proteins. We also show that targeting to Tat⁺-INV requires an intact RR motif in the signal sequence. The combined results are consistent with the bacterial TatABC pore recognizing at least the consensus motif of the RR signal sequence. In accordance, the TatB and TatC analogs of thylakoids were recently shown to represent a Tat-specific recognition site from which translocation proceeds in a TatA-dependent manner (29).

It should be noted that the Tat precursors tested throughout these studies are known not to require bound cofactors for export (15, 27). Nevertheless, their translocation into INV required Tat-specific conditions such as the presence of TatABC, the H⁺ gradient, and the RR motif. By use of low salt-stripped INV, we have blocked RR-dependent translocation without interfering with a functional membrane targeting of Tat precursors. This strategy should now enable us to characterize both events individually, applying INV derived from Tat deletion strains and cross-linking methods.

	FOOTNOTES

^* This work was supported by Grant QLK3-CT-1999-00917 of the European Union (to L. F. W. and M. M.) and by support from the Sonderforschungsbereich 388 and the Fonds der Chemischen Industrie (to M.M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 49-761-203-5265; Fax: 49-761-203-5274; E-mail: matthias.mueller@biochemie.uni-freiburg.de.

Published, JBC Papers in Press, March 28, 2002, DOI 10.1074/jbc.M201711200

	ABBREVIATIONS

The abbreviations used are: Tat, twin-arginine translocation; TeaOAc, triethanolamine acetate; INV, inside-out inner membrane vesicles; CCCP, carbonyl cyanide m-chlorophenyl-hydrazone; DTT, dithiothreitol; LSE, low salt extract.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

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