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J. Biol. Chem., Vol. 277, Issue 27, 24103-24113, July 5, 2002
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From the
Received for publication, January 29, 2002, and in revised form, March 14, 2002
We have identified and defined the function of
kpsF of Neisseria meningitidis and the
homologues of kpsF in encapsulated K1 and K5
Escherichia coli. KpsF was shown to be the
arabinose-5-phosphate isomerase, an enzyme not previously identified in
prokaryotes, that mediates the interconversion of ribulose 5-phosphate
and arabinose 5-phosphate. KpsF is required for
3-deoxy-D-manno-octulosonic acid (Kdo) biosynthesis in
N. meningitidis. Mutation of kpsF or the gene
encoding the CMP-Kdo synthetase (kpsU/kdsB) in
N. meningitidis resulted in expression of a
lipooligosaccharide (LOS) structure that contained only lipid A and
reduced capsule expression in the five invasive disease-associated
meningococcal serogroups (A, B, C, Y, and W-135). The step linking
meningococcal capsule and LOS biosynthesis was shown to be Kdo
production as the expression of capsule was wild type in a Kdo
transferase (kdtA) mutant. Thus, in addition to
lipooligosaccharide assembly, Kdo is required for meningococcal
capsular polysaccharide expression. Furthermore, N. meningitidis, unlike enteric Gram-negative bacteria, can survive and synthesize only unglycosylated lipid A.
Neisseria meningitidis, an exclusive pathogen of
humans, is the cause of epidemic bacterial meningitis and sepsis.
Capsular polysaccharide and lipooligosaccharide
(LOS1 or endotoxin) are
critical virulence factors in meningococcal pathogenesis. For example,
both structures contribute to the resistance of meningococci to
bactericidal activity of human sera (1, 2). Structural differences in
capsule and LOS determine meningococcal serogroups and immunotypes,
respectively. Of the 13 different capsule serogroups so far defined, 5 serogroups (A, B, C, Y, and W-135) cause the majority of cases of
invasive meningococcal disease. With the exception of the serogroup A
capsule, ( Meningococcal LOS consists of a lipid A moiety, a conserved inner core
composed of two heptoses linked to two Kdo sugars, and an outer core
with variable oligosaccharide composition. The meningococcal lipid A is
distinct from that of Escherichia coli and is composed of a
The genetic basis of meningococcal capsule expression has been studied
extensively. The capsule (cps) locus consists of a four-gene
biosynthesis operon that encodes the production of sialic acid or
N-acetylmannosamine and the formation of capsule polymers, whereas the divergently transcribed ctrABCD transport operon
encodes proteins responsible for capsule translocation (11-13). Nearby are two genes, lipA and lipB, proposed to be
involved in lipidation of capsule polymers (14). No genes outside the
cps locus have been shown to participate in meningococcal
capsule expression. E. coli K1 strains also express a
capsule composed of Materials and Bacterial Strains
Bacterial strains, plasmids, and primers used in this study are
described in Table I. Monoclonal
antibodies for meningococcal serogroup B (2-2-B), C (4-2-C), Y (5-2-Y),
and W-135 (7-1-W) capsular polysaccharides were kindly provided by
Wendell Zollinger (Water Reed Army Institute of Research, Washington,
D. C.). Monoclonal antibody M2 against FLAG epitope and antibiotics
were obtained from Sigma. Restriction enzymes were purchased from New
England Biolabs. Polyclonal antiserum to the KpsF protein was raised in rabbits (Covance Co.).
Growth Conditions
Meningococcal strains were grown with 3.5% CO2 at
37 °C unless specified otherwise. GC base agar (Difco), supplemented
with 0.4% glucose and 0.68 mM
Fe(NO3)3, or GC broth with same supplements and
0.043% NaHCO3 was used. BHI medium (37 g/liter brain heart infusion) with 1.25% fetal bovine serum was used when kanamycin selection was required. Antibiotics concentrations (in µg/ml) used
for E. coli strains were ampicillin, 100, kanamycin, 50, spectinomycin, 100, and erythromycin, 300; and for N. meningitidis were kanamycin, 80, spectinomycin, 60, erythromycin,
3, and tetracycline, 5. E. coli strain DH5 Construction of Meningococcal Nonpolar Mutants
kpsF--
An internal 793-bp fragment of kpsF
(NMB0352) was produced by PCR amplification using primers YT60 and YT61
and cloned into pCR2.1 to yield pYT203. A SmaI-digested
aphA-3 cassette (19) was subsequently inserted into the
unique AscI site (blunted with Klenow) to generate pYT206.
ScaI-linearized plasmid was used to transform meningococcal
strain NMB. The correct homologous recombination of the
aphA-3 cassette into NMB0352 coding sequence was confirmed by PCR.
kdsB--
The NMB0675 (kdsB) sequence from the MC58
genome (20) was used to design primers YT84 and YT85. A 746-bp PCR
product was amplified from chromosomal DNA of strain NMB using Vent DNA
polymerase (New England Biolabs), phosphorylated with T4 kinase, and
then cloned into HincII-SmaI-digested pUC18 to
yield pYT256. The aphA-3 cassette released by
SacI-HincII digestion was subsequently inserted into the unique EcoRV site within kdsB to
generate pYT259. Colony PCR using KanC (21) and YT85 primers confirmed
the correct insertion of the cassette. ScaI-digested pYT259
was used to transform strain NMB, and kanamycin-resistant colonies were
selected at 30 °C.
tal--
PCR amplification using primers YT68 and YT69 and NMB
chromosomal DNA as a template yielded a 715-bp internal fragment of tal, and the PCR product was subsequently cloned into
pCR2.1. A unique ClaI site was used to insert the
aphA-3 cassette released by
EcoRI-BamHI double digestion and blunted with
Klenow. The resulting plasmid, pCAS5, with correct orientation and
in-frame fusion of the aphA-3 in tal was
linearized with ScaI digestion and used for transforming
meningococcal strain NMB to generate the CAS5 mutant.
kdtA--
The construction of this mutant has been described
previously.2
Overexpression and Purification of the Meningococcal KpsF
Protein
The coding sequence of KpsF was amplified with primers YT70 and
YT71. The PCR product was digested with NdeI and
XhoI and then cloned into pET20b(+) which had been digested
with the same enzymes, yielding pYT225. The plasmid was purified and
transformed into E. coli expression strain, BLR21(DE3)pLysS.
One liter of LB culture of the KpsF overexpression strain was induced
with 1 mM IPTG. The harvested cells were resuspended in 15 ml of lysis buffer (50 mM sodium phosphate, pH 8.0; 300 mM NaCl; 10 mM imidazole; 1 mM
phenylmethylsulfonyl fluoride) and sonicated 10 times for 30 s
with 30-s cooling intervals. The cell debris was removed by
centrifugation at 14,000 × g for 15 min. The crude
extract was then incubated with 2 ml of 50% suspension of
nickel-nitrilotriacetic acid resin (Qiagen) for 2 h before packing
into a column. The column was washed with 10 ml each of 20 and 50 mM imidazole in lysis buffer and then eluted with 10 ml of
250 mM imidazole. The fractions were pooled after SDS-PAGE
analysis, concentrated through a Centricon-3 filter (Amicon), and
dialyzed in storage buffer (50 mM HEPES, pH 7.5; 100 mM NaCl; 5 mM MgCl2; 1 mM EDTA). Protein concentration was determined by the
Bradford assay (Bio-Rad) with bovine serum albumin as standards.
Complementation of the NMB206 Mutant by a Second Copy of
Meningococcal kpsF
A plasmid containing an intact copy of kpsF
controlled by the lac promoter and ermC gene
inserted downstream of kpsF was constructed. Full-length
kpsF was amplified with primers CAS1 and CAS2. The PCR
product was digested with HindIII and BamHI and
cloned into pEGFP cut with the same enzymes. An erythromycin-resistant
cassette (ermC) obtained from pAErmC'G (22) with
EcoRI digestion was subsequently cloned downstream of the
kpsF gene using SmaI-EcoRI sites. This
kpsF/ermC construct was amplified by PCR with primers CAS4
and YT59 and then cloned into the unique HincII site within a 1-kb chromosomal sequence of meningococcal 120A1 locus, which is
located about 85 kb from the kpsF locus, in pYT109 (23). Transformation and homologous recombination of the flanking sequences of the 120A1 locus introduce the kpsF/ermC fragment into
this site and resulted in ermR transformants. Subsequently,
a PCR product (primers YT69 and CAS3) encompassing the sequence
flanking the entire kpsF coding sequence with the
aphA-3 insertion within kpsF was used to
transform this strain. ErmR/KanR transformants
were then selected. A panel of PCR analyses and Southern blots with
aphA-3 cassette and kpsF internal fragment (YT60-YT61) as probes confirm that allelic exchange of the
aphA-3 cassette occurred at the wild type kpsF
locus, and the second copy of kpsF at the 120A1 locus was
intact (data not shown).
Cloning of the K1 E. coli kpsF Homologue
Primers YT77 and YT78 were used to amplify kpsF from
K1 E. coli strain EV36 (24). The PCR fragment was digested
with EcoRI and BglII and ligated with pFlag-CTC
(Sigma), which has been cut with the same enzymes. kpsF was
therefore under the control of the tac promoter and was
fused to the FLAG epitope in the resulting plasmid, pYT239. A fragment,
which contains the lacI repressor gene and the cloned
kpsF, was released by BglI digestion and then subcloned into the EcoRV site of a meningococcal shuttle
vector pYT250 to yield pYT240. The kpsF-coding sequence with
in-frame FLAG fusion was confirmed with DNA sequencing analysis. The
kpsF-encoding plasmid was methylated with HaeIII
methylase according to the reported procedure (25) prior to
transformation into meningococci. Erythromycin-resistant transformants
were analyzed for the presence of E. coli kpsF by colony PCR.
LOS Extraction and Characterization
Twelve liters of meningococcal cultures were harvested, and the
combined cell pellet was dried in a SpeedVac (Savant) overnight, and
the dry weight was measured. The dried pellet was then extracted with
phenol:chloroform:petroleum ether as described (21). The LOS samples
were analyzed with 16.5% Tricine SDS-PAGE followed by silver staining
(26). A micro phenol/water extraction was done as described below. A
2-ml aliquot of cultures at ~0.9 of A600 reading was collected, and the
bacterial pellet was resuspended in 0.5 ml of buffer A (50 mM Na2HPO4, pH 7.0; 5 mM EDTA; 0.05% NaN3). A 0.5-ml aliquot of 90%
liquefied phenol was added to the cell suspension and mixed by vortex.
The mixture was incubated at 65 °C for 15 min with vortex every 5 min and then cooled on ice for another 5 min. The aqueous phase and the
phenol phase were separated by centrifugation. Both phases were
dialyzed (6000-8000 Mr cut-off) against 5 changes of water and then lyophilized. An extraction method using a
solution of 0.25 M EDTA and 0.25 M of TEA was
adapted (27). Cells from 1.5 ml of overnight cultures were resuspended
in 50 µl of EDTA-TEA buffer or EDTA-TEA, 5% phenol and incubated at
60 °C for 30 min. The crude LOS in the supernatant was collected
after centrifugation.
A mini-scale LOS preparation was obtained by proteinase K
treatment of whole cell lysates. Briefly, cells were suspended in water, and the protein concentrations were estimated by Bradford assay.
A mixture of 8 µl of whole cell lysate at a concentration of 1 µg/µl, 28 µl of 2% SDS in TE buffer, and 8 µl of proteinase K
(25 mg/ml) was incubated at 60 °C overnight. The digestion was quenched by adding 38 µl of loading buffer (21). Aliquots of LOS
samples were resolved on a Tricine SDS minigel, and the LOS migration
pattern was visualized by silver staining (26).
Structural Analysis of LOS of the NMB206 Mutant
The contaminant phospholipids in the LOS samples extracted from
the phenol:chloroform:petroleum ether method were removed by extracting
the LOS with ethanol:water (9:1, v/v). The supernatant was removed, and
the pellet was extracted repeatedly until no more phospholipid was
found in the supernatant. The level of phospholipid was determined by
the amount of C16:0, C16:1, and C18:1 fatty acids present because they
are characteristic of meningococcal phospholipids (28). The resulting
pellet was suspended in water and freeze-dried.
Compositional analysis was performed by the preparation and combined
gas chromatographic/mass spectrometric analysis of
trimethylsilylmethylglycosides with N-acetylation and of
fatty acid methyl esters (29). For the determination of Kdo, lipid A
was methanolyzed with methanolic 1 M HCl at 80 °C for
4 h (30) prior to trimethylsilylation and GC-MS analysis.
Ester-linked fatty acids were selectively liberated from a vacuum-dried
sample by alkaline transesterification with sodium methoxide (0.25 M, 37 °C, 15 h) (31). Combined GC-MS analysis was
performed using a 50-m methylsilicone column from Quadrex Corp.
Methylation analysis was carried out using the method of Ciucanu and
Kerek (32). The permethylated product was purified by using a Sep-Pak
C18 cartridge (33), then hydrolyzed with 2 M
trifluoroacetic acid (120 °C, 2 h), reduced with
NaB2H4, acetylated, and analyzed by combined
GC-MS.
Mass Spectrometry of LOS
To dephosphorylate lipid A, the sample was treated with cold
aqueous 48% hydrogen fluoride (HF) and kept for 48 h at 4 °C. The HF was removed by flushing under a stream of air, followed by
addition of diethyl ether (600 ml) and drying with a stream of air.
This latter diethyl ether/drying step was repeated three times. The
resulting residue was suspended in deionized water, dialyzed at 4 °C
for 48 h, and finally freeze-dried.
Oligosaccharides were analyzed by MALDI-TOF mass spectrometry using a
Hewlett-Packard LD-TOF system. The oligosaccharides were dissolved in
distilled water at a final concentration of 2 µg/µl, and 1 µl was
mixed with the dihydroxybenzoic acid in methanol matrix for analysis.
Tandem MS/MS analysis was performed using a Q-TOF hybrid mass
spectrometer (Q-TOFII; Micromass, UK) equipped with an electrospray source (Z-spray) operated in the either the positive or negative mode.
The samples were dissolved in 1:1 methanol and chloroform and infused
into a mass spectrometer with a syringe pump (Harvard Apparatus
Cambridge, MA) at a flow rate of 5 µl/min. A potential of 3 kV (+ or
Immunoblots
The detailed colony immunoblot protocol has been published
previously (21). Briefly, cells resuspended in GC broth were diluted to
2 × 108 cells/ml
(A550 nm = 0.4). Aliquots of 50 µl of
the cell suspensions at various dilutions were applied to a pre-wetted nitrocellulose membrane. The membrane was blocked with 3% bovine serum
albumin for 1 h. Monoclonal antibodies specific for serogroups B
(2-2-B), C (4-2-C), W-135 (7-1-W), and Y (5-2-Y) were used at 1:500,
1:500, 1:500, and 1:50 dilution, respectively.
Protein samples for Western blots were resolved by 12% SDS-PAGE and
transferred to polyvinylidene difluoride membranes. 10% bovine serum
albumin in TTBS was used to block the membrane. Anti-FLAG monoclonal
antibody was used at 10 µg/ml in TTBS. Anti-KpsF polyclonal serum
(Covance) was used at a 1:500 dilution.
Whole Cell ELISA
The previously published protocol of whole cell ELISA (12) with
minor modification was employed. A 50-µl aliquot of a 1:3 dilution of
cell suspensions at A650 of 0.1 was applied and
dried at 37 °C overnight. Antibodies specific for serogroup B
(2-2-B) and serogroup A (14-1-A) were used at 1:2000 and 1:30,000
dilution, respectively. All incubations were performed at 37 °C.
Determination of Ketopentoses
The procedure of Dische and Borenfreund (34), as modified by
Bigham et al. (35), was used to determine the presence of ketopentoses. Briefly, enzyme was incubated at 37 °C in the presence of various aldopentoses (ribose 5-phosphate, erythrose 4-phosphate, glucose 6-phosphate, and arabinose). The reaction solution was quenched
with the addition of 1.5% cysteine solution followed immediately by
concentrated H2SO4. An aliquot of 0.12%
carbazole in 95% ethanol was then added, and the absorbance was read
at 540 nm. As reported by Ray and co-workers (35), the conversion of 1 µmol of arabinose 5-phosphate (Ara-5-P) to 1 µmol of
ribulose 5-phosphate gave an 31P NMR Analysis
To a solution of phosphorylated pentose (2.7 mM
Ara-5-P or 5.4 mM ribulose 5-phosphate), 100 mM
Bis-tris propane-HCl, pH 7.5, and 10% D2O (for NMR lock)
in a 3-mm NMR tube was added to ~25 pmol of Ara-5-P isomerase. This
solution was then monitored by 31P NMR until equilibrium
(no further change in peak ratio) was achieved. Samples are referenced
to an external standard of neat phosphoric acid (0 ppm). Spectra were
obtained on a Brucker Avance DRX-500 operating at 202.46 MHz for
31P with WALTZ16 proton decoupling. Each spectrum
represents 64 scans.
Statistical Analysis
Student's t test with a two-tailed hypothesis was
used to determine the significant difference (p Identification of kpsF Homologue in N. meningitidis--
The first
gene of region 1 of the E. coli K1 kps locus,
kpsF, is absent in the meningococcal cps locus
(Fig. 1). A search of the complete
serogroup B (MC58) and A (Z2491) genome data bases (20, 39) with the K1
KpsF protein sequence revealed a single highly homologous gene in each
genome, NMB0352 and NMA2135, respectively. In contrast to K1 E. coli, the kpsF homologues in both genomes were not
associated with the capsule locus. For example, in serogroups B MC58
genome, the capsule locus (NMB0067-NMB0083) is centered around 80 kb,
whereas NMB0352 is located near 360 kb. A divergently transcribed gene
encoding a putative transaldolase (tal) was located 96 bp
upstream of the kpsF homologue, and a 221-bp intergenic space separated kpsF from a gene (NMB0353) downstream
predicted to encode a conserved hypothetical protein (Fig. 1). NMB0352
(kpsF) was predicted to encode a 34-kDa protein of 324 residues and to be a cytoplasmic soluble protein by topology prediction
programs, TopPredII and Psort (40, 41). The NMA2135 homologue of
serogroup A genome was in an identical organization to that of NMB0352
(Fig. 1) and shared 98% identity. NMB0352 is annotated as a sugar
isomerase in the MC58 genome because of the presence of a sugar
isomerase domain (42) between residues 38 and 172. A putative Walker A box is also located within the sugar isomerase domain. Unlike N. meningitidis, E. coli contained two additional
kpsF homologues, gutQ and yrbH.
An alignment of the five predicted proteins is shown in Fig.
2. The homology was shared throughout the
entire protein sequence, especially the sequence surrounding the Walker
A box within the sugar isomerase domain. Both meningococcal KpsF
homologues have slightly higher similarity to YrbH than to either
E. coli KpsF or QutQ (67 versus 60%).
Mutation of kpsF Yielded a Defect in Capsule Expression in the Five
Invasive Disease-associated Meningococcal Serogroups--
Because
kpsF has been proposed to participate in K1 capsule
expression (43), the meningococcal kpsF homologue was
mutated in the serogroup B meningococcal strain NMB. The capsule
phenotype in multiple independent nonpolar
kpsF::aphA-3 transformants
(n = 4) was assayed by a serogroup B capsule-specific
whole cell ELISA. Only 10-20% of the serogroup B capsule expressed by
the parent strain was expressed by the transformants. The data for one
of these transformants, designated NMB206, are shown in Fig. 3A. The reduction of capsule
in the NMB206 mutant and other
kpsF::aphA-3 transformants was further
confirmed by colony immunoblots (Fig. 3B). The
capsule-deficient phenotype was also reproduced in four independent
transformants of strain NMB using a PCR product containing only the
aphA-3 cassette and kpsF-flanking DNA, amplified
from the NMB206 mutant using primers YT60 and YT61 (Fig. 1).
Cieslewicz and Vimr (43) reported that mutation of kpsF
results in an intracellular accumulation of capsular polymers in a
K1-K12 hybrid E. coli strain. To determine whether the
meningococcal kpsF::aphA-3 mutant
accumulated intracellular capsular polysaccharide, mutant NMB206 was
lysed by freeze-thaw treatment or by an EDTA-HEPES method described by
Moe et al. (44). An E. coli strain, EV94 (kpsS::Tn10), which has been shown to
accumulate intracellular capsule (45), was included as a positive
control for lysis. Capsular polysaccharide released into the
supernatant was quantified by ELISA. Additional capsular polysaccharide
was not detected by either lysis method when compared with the whole
cell ELISA, indicating intracellular capsule polymers did not
accumulate in a meningococcal kpsF mutant (data not shown).
The gene for a putative transaldolase (tal) was found to be
immediately upstream of kpsF and was transcribed divergently
from kpsF with a 96-bp intergenic space. A nonpolar
aphA-3 insertional mutation was created in tal, whose product is predicted to function in the pentose phosphate metabolic pathway (46), and the resulting mutant expressed a wild type
level of capsule as measured by capsule whole cell ELISA (data not shown).
To assess whether the meningococcal KpsF homologue was required for
meningococcal capsule expression of the major disease-associated serogroups, strains of serogroup A (strain F8229), C (FAM18), Y
(GA0929), and W-135 (GA1002) of N. meningitidis were
transformed with linearized pYT206, and the mutation within
kpsF in all strains was confirmed by PCR. Capsule expression
was then assessed by whole cell ELISA (serogroup A) or colony
immunoblots (serogroups C, Y, and W-135) by using capsule
serogroup-specific monoclonal antibodies. Reduced capsule expression
was observed in strains of all serogroups (Fig. 3B). These
results demonstrated that KpsF was required for the expression of
either the sialic acid (serogroups B, C, Y, and W-135) or non-sialic
acid (serogroup A) meningococcal capsules.
Lipo-oligosaccharide Was Truncated in the kpsF Mutant and Contained
Only Unglycosylated Lipid A--
The kpsF (NMB206) mutant
formed small crinkled colonies that had a dry-rough appearance. To
access whether other outer membrane structures were altered in the
mutant, whole cell lysates and outer membrane preparations were
examined. No major alterations in proteins were observed in these
preparations. However, proteinase K-treated whole cell lysates examined
by Tricine SDS-PAGE followed by silver staining revealed no LOS. To
understand these observations, we used four different LOS extraction
methods as follows: phenol/chloroform/petroleum ether, hot
phenol/water, EDTA-TEA/proteinase K, and EDTA-TEA, 5% phenol. As shown
in Fig. 4, no silver-staining bands
corresponding to LOS of the wild type parent were observed in material
obtained from any of these extractions. As a LOS structure containing
lipid A-Kdo2 (mutant 469 (1, 47)) can be detected by silver
staining, these data suggested that the LOS structure was further
truncated or that no LOS was produced by the meningococcal
kpsF mutant.
Fatty acid analysis of the material extracted by
phenol/chloroform/petroleum ether revealed the presence of
approximately equal molar amounts of dodecanoic acid (C12:0, 980 nmol/mg), 3-hydroxydodecanoic acid (3-OHC12:0, 965 nmol/mg), and
3-hydroxytetradecanoic acid (3-OHC14:0 940 nmol/mg). A small amount of
palmitic acid (C16:0) was also observed which was not part of the LOS
structure (see below) and, perhaps, was due to the presence of a low
level of contaminating phospholipids. The same fatty acyl residues were present in the same ratio in HF-treated LOS, except that in this case a
significant level of GlcN was also detected (925 nmol/mg). Assuming a
normal lipid A structure which would have 2 mol of GlcN per mol of
lipid A, it can be concluded that there are a total of 6 mol of fatty
acid per mol of lipid A, i.e. ~2 mol each of C12:0,
3-OHC12:0, and 3-OHC14:0. After treatment of lipid A with sodium
methoxide, C12:0 and 3-OHC12:0 were quantitatively liberated as methyl
esters, showing that they had been exclusively ester-linked. The mild
alkaline-treated LOS was subjected to strong alkaline hydrolysis which
released only 3-OHC14:0 and proved that this was the amide-bound fatty
acyl residue. Thus, composition analysis suggests that NMB206 produces
a LOS with the expected lipid A for N. meningitidis.
However, what proved to be very unusual was that the LOS contained no
detectable glycosyl components other than the GlcN that is derived from
the lipid A. In fact, none of the glycosyl residues typical of LOS from
the wild type NMB or other mutants (8) was detected including the inner
core sugar residues, heptose and Kdo. These results suggested that the
LOS from NMB206 consisted only of unglycosylated lipid A because no
glycosyl residues could be detected and because it contained the
typical fatty acylation pattern for meningococcal lipid A.
LOS from NMB206 was further analyzed by mass spectrometry, MALDI-TOF
MS. The results are shown in Fig.
5A. The [M
The above MALDI-TOF results showed that NMB206 produced one major LOS
molecule, i.e. at m/z 1633, which consisted of
N. meningiditis lipid A with only one phosphate. This LOS
was completely devoid of any of the core glycosyl residues including
Kdo. The results also suggested that the one phosphate group is located
at the 4'-position and that there is no glycosidically linked
phosphate. In order to confirm the location of the phosphate, the LOS
was methylated, and partially methylated alditol acetates were prepared and analyzed by GC-MS. In this procedure the GlcN residues that are
phosphorylated at the 4'-position retain the phosphate in their
partially methylated alditol acetate derivative and are not observed
during GC-MS analysis, whereas the reducing-end GlcN, or
GlcN-1-phosphate, residues of the lipid A are observed as the partially
methylated alditol acetate of 6-linked GlcN (8). Because there was no
detectable terminally linked GlcN, these results support the conclusion
that the 4'-position in the LOS is phosphorylated and, therefore, the
single phosphate group on this LOS must be located at the
4'-position.
The structure of the LOS, after removal of the phosphate substituent,
was further analyzed by tandem MS/MS analysis, Fig. 6A. The [M + Na]+ ion, m/z 1576, gives primary fragments due
to the following: (a) the loss of either
From the above results, it is clear that the LOS from NMB206 consists
primarily of lipid A that is not glycosylated and contains a single
phosphate group at the 4'-position. There are minor species in which
this phosphate is substituted by a phosphoethanolamine group
(m/z 1756), lacks one of the fatty acyl substituents
(m/z 1435 and 1451), or consists of a monophosphorylated
triacylglucosamine residue (m/z 864). The structure of the
major LOS from the kpsF mutant (NMB206) is shown in Fig.
6B.
Complementation of the Meningococcal kpsF Mutant--
To confirm
that the observed phenotypes were due to mutation of kpsF,
complementation experiments were performed to introduce a second intact
copy of kpsF into the meningococcal NMB206 (kpsF) mutant. To overcome decreased competence of the NMB206 mutant, we
transformed strain NMB with the plasmid carrying a second copy of
kpsF prior to the inactivation of the wild type gene with
the aphA-3 insertion (see "Experimental Procedures").
Only transformants in which PCR and Southern blots confirmed that
aphA-3 insertion occurred in the chromosomal copy but not
the plasmid-encoded copies of kpsF were selected for further
studies. When capsule expression was examined by whole cell ELISA and
colony immunoblots, the complemented strain was identical to the wild
type (data not shown), indicating mutation of the wild type copy of
kpsF was complemented by the presence of the second copy of
kpsF.
The meningococcal KpsF protein shares 64% similarity to the E. coli K1 KpsF protein. To determine whether these two proteins were
homologues, we first constructed a strain (NMB240) carrying pYT240, an
ErmR shuttle vector containing the E. coli K1
kpsF fused to a FLAG epitope and under the control of the
tac promoter. We then disrupted the meningococcal
kpsF using a PCR fragment (primers YT60 and YT61) amplified
from the NMB206 mutant that contained the aphA-3 cassette
within the kpsF coding sequence. ErmR and
KanR transformants (NMB240/206) were identified that
contained the insertion of aphA-3 cassette into
meningococcal kpsF and an intact copy of E. coli
K1 kpsF on the shuttle vector. A strain (NMB250) containing
the shuttle vector without the E. coli kpsF insert (pYT250)
was used to generate a negative control strain (NMB250/206) for these experiments.
Whole cell lysates of strains NMB206, NMB240, NMB240/206, NMB250, and
NMB250/206 were analyzed by Western blots probed with an anti-FLAG
monoclonal antibody (Fig. 7A)
and anti-KpsF polyclonal serum (Fig. 7B). K1 KpsF-FLAG
proteins were expressed at a similar level in both the NMB240 and
NMB240/206 strains without induction, indicating an incomplete
suppression by lacI. Increased expression was observed in
the presence of IPTG (Fig. 7A). The absence of reactive band
in strains containing kpsF::aphA-3
mutations, when probed with antiserum against meningococcal KpsF,
confirmed that these mutations eliminated expression of meningococcal
KpsF (Fig. 7B). The colony morphology and growth rate of the
NMB240/206 strain was similar to the wild type parent, whereas the
NMB250/206 strain resembled the NMB206 strain. When these strains were
examined by the capsule-specific whole cell ELISA, the NMB240/206
complemented strain yielded wild type level of capsule expression,
whereas the NMB250/206 negative control produced a capsule-deficient
phenotype similar to that of strain NMB206 (Fig. 7D). These
data demonstrated that E. coli K1 KpsF could functionally
replace the meningococcal KpsF and complement the capsule-deficient
phenotype of the NMB206 mutant. LOS from these strains was also
examined by silver-stained Tricine SDS-PAGE, and wild type LOS bands
were restored in the NMB240/206 strain but not the NMB250/206 strain,
indicating complementation of meningococcal kpsF mutation by
E. coli K1 KpsF (Fig. 7C).
Kdo Biosynthesis Is Involved in Meningococcal Capsule
Expression--
LOS is the major component of the outer leaflet of the
outer membrane, and capsule is anchored on the outer membrane via a diacylglycerol moiety (7). Possible structural changes of the outer
membrane produced by the markedly truncated LOS (unglycosylated lipid
A) might affect capsule expression, although capsule expression was not
influenced in previous meningococcal mutants with various truncations
in the outer and inner core of LOS (1) or in a lpxA mutant
that does not contain LOS.3
To address this question, a kdtA mutation was created in
meningococcal strain NMB yielding strain NMB249. KdtA is the CMP-Kdo
transferase that catalyzes the transfer of Kdo to lipid A. The NMB249
mutant generated the same unglycosylated lipid A structure as that of the kpsF mutant.2 However, this mutant, when
analyzed by whole cell ELISA, produced wild type levels of capsule
(Fig. 8). Thus, the reduced capsule expression caused by the kpsF mutation was not due to outer
membrane alterations or other pleiotropic effects resulting from a
truncated LOS.
The absence of Kdo in LOS and the reduction of capsule expression in
the kpsF mutant suggested a role of Kdo in capsule
expression. Kdo has been identified as a component of the E. coli K5 capsule at the reducing end of the polymer (48). To assess
further the role of Kdo in meningococcal capsule expression, an
insertional nonpolar mutation was created in the CMP-Kdo synthetase,
kdsB, in order to eliminate the production of the activated
Kdo sugar. When the kdsB mutant was assayed by whole cell
ELISA, a significant reduction in capsule expression, similar to that
of the kpsF mutant, was observed (Fig. 8). These data showed
a role for Kdo in meningococcal capsule expression and further
suggested that KpsF was involved in Kdo production.
KpsF Is the Arabinose-5-phosphate Isomerase of N. meningitidis--
The predicted protein sequence of KpsF suggested a
sugar isomerase activity. To determine whether KpsF had an isomerase
activity, a colorimetric assay system for keto-pentoses (35) was first conducted using the purified KpsF protein, which contained a C-terminal His6 tag (Fig.
9A). Addition of KpsF protein
into the reaction mixture containing arabinose 5-phosphate resulted in
an increase in color, whereas adding the protein to ribulose
5-phosphate caused a decrease in reading of A540
(data not shown). The reaction was dependent on the concentration of
KpsF protein. Other monosaccharides, such as erythrose 4-phosphate,
glucose 6-phosphate, ribose 5-phosphate, and arabinose, did not serve
as substrates for KpsF protein (data not shown). The NMR chemical
shifts of the phosphoryl groups of arabinose-5-P and ribulose-5-P are
different; thus 31P NMR can monitor the interconversion
between the two sugars. As shown in Fig. 9C, using
arabinose-5-P as the substrate, a new peak corresponding to the
phosphoryl group of ribulose-5-P appeared and increased over time, and
the same phenomenon was observed for the reverse reaction. These data
demonstrated that KpsF catalyzes the interconversion of ribulose
5-phosphate and arabinose 5-phosphate. The LOS defect in the
kpsF mutant, but not the kdsB mutant, can be
complemented by exogenous supplement of arabinose in the growth medium
(Fig. 9B), further indicating the role of KpsF protein as an
arabinose-5-phosphate isomerase.
By using a comparative genomic approach based on the two recently
published meningococcal genome data bases (39, 40), we identified
meningococcal homologues of kpsF and kpsU
(kdsB) of K1 E. coli. In contrast to K1 E. coli, N. meningitidis contains only a single copy of
the gene encoding the CMP-Kdo synthetase, kdsB, in a
location distant from the capsule locus. We found that the
meningococcal kpsF and kpsU (kdsB)
homologues are both required for Kdo biosynthesis. KpsF was identified
as the arabinose-5-phosphate isomerase. Mutations of kpsF
and kpsU (kdsB) in N. meningitidis caused a significant reduction in surface capsule expression and resulted in a LOS structure composed only of unglycosylated
meningococcal lipid A.
The condensation of arabinose 5-phosphate and phosphoenolpyruvate
catalyzed by the Kdo synthase, KdsA, is usually considered to be the
first step in Kdo biosynthesis (Fig.
10). However, arabinose 5-phosphate is
not readily available from glycolysis, and an isomerase is required for
the interconversion of ribulose 5-phosphate and arabinose 5-phosphate
(Fig. 10). Although the enzymatic activity has been demonstrated in
cell extract, the gene encoding this enzyme has not been identified
previously (35). KpsF contains a sugar isomerase domain commonly found
in a variety of proteins such as GlmS and LpcA (42) involved in
phosphosugar isomerization. In addition, the sugar isomerase domain is
also present in the transcriptional regulator, RpiR, of the
ribose-phosphate isomerase, RpiA, which interconverts ribose
5-phosphate and ribulose 5-phosphate, a reaction that precedes the
arabinose-5-phosphate isomerase. Interestingly, in addition to
kpsF, region 1 of the capsule locus of E. coli
strains expressing group II capsules (e.g. K1 and K5) encodes a second copy of the CMP-Kdo synthetase (kpsU). In
E. coli, this may be an evolutionary acquisition of genes to
ensure that the Kdo substrate required for capsule biosynthesis was not limited by requirements of the LPS biosynthesis pathways. In fact in
E. coli two other predicted proteins, GutQ and YrbH, are
homologues of KpsF. GutQ is located within the glucitol operon, but its
function has not been determined previously (49).
KpsF Is the Arabinose-5-phosphate Isomerase Required for
3-Deoxy-D-manno-octulosonic Acid Biosynthesis and for
Both Lipooligosaccharide Assembly and Capsular Polysaccharide
Expression in Neisseria meningitidis*
,,
,**
Division of Infectious Diseases, Department
of Medicine, Emory University School of Medicine, the
** Department of Veterans Affairs Medical Center,
Atlanta, Georgia 30033, the § Complex Carbohydrate
Research Center, University of Georgia, Athens, Georgia 30602, and
the ¶ Department of Medicinal Chemistry, University of Michigan,
Ann Arbor, Michigan 48109
ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
1
6)-linked N-acetylmannosamine 1-phosphate,
meningococcal capsules associated with invasive diseases contain sialic
acid as follows: serogroup B, 28-linked
N-acetylneuraminic acid (NeuAc); serogroup C,
29-linked partially O-acetylated NeuAc;
serogroup Y, an alternating sequence of D-glucose and
partially O-acetylated NeuAc; and serogroup W-135, an
alternating sequence of D-galactose and NeuAc (3-6). Each
of the capsular polymers also contains a 1,2-diacylglycerol
phospholipid anchor (7).
1',6-linked disaccharide of glucosamine acylated with
-hydroxymyristates and -hydroxylaurates at 2- and 2'-, and 3- and
3'-positions respectively, and symmetrical acyloxyacyl linkages of
laurate residues are located at 2- and 2'-positions (8). More than 30 genes involved in the biosynthesis of lipid A, heptose, Kdo, and the
outer core polysaccharides have been identified (9), but the genes
required for the assembly of Kdo are not completely defined (10).
28-linked polysialic acid. The
kps locus of K1 E. coli capsule expression has
also been well characterized and, when compared with the meningococcal locus (Fig. 1A), contains several "extra" genes
including kpsF, kpsD, kpsU,
neuD, and neuE (15). KpsU has been shown to
encode a second copy of the CMP-Kdo synthetase, KdsB (16). KpsD is a
periplasmic protein, and mutation of kpsD results in
periplasmic accumulation of capsular polysaccharide (17). The functions of KpsF, NeuD, and NeuE are currently unknown. Furthermore, it is not
known whether these genes are present in meningococci and whether they
play a role in meningococcal capsule expression.
EXPERIMENTAL PROCEDURES
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
Bacterial strains, plasmids, and primers used in this study
cultured on
Luria Bertani (LB) medium was used for cloning and propagation of
plasmids. Meningococci were transformed by the procedure of
Janik et al. (18). E. coli strains were
transformed by electroporation with GenePulser (Bio-Rad) according to
the manufacturer's protocol.
) was applied to the capillary, and nitrogen was employed as both the
drying and nebulization gas. NaI and [Glu]fibrinopeptide B were used
as calibration standards in the negative and positive modes,
respectively. In the MS analysis the Q1 is operated in RF-only mode
with all ions transmitted into the pusher region of the TOF analyzer,
and the MS spectrum was recorded from m/z 400-2000 with 1-s
integration time. For MS/MS spectra, the transmission window of
quadrupole (Q1) was set up to about 3 mass units, and the selected
precursor ions were allowed to fragment in the hexapole collision cell.
The collision energies (40-55 eV) were optimized for maximized product
ion yield, and argon was used as collision gas. The MS/MS data were
integrated over a period of 4-5 min for each precursor ion.
A of 8.2.
0.05) between two variables in this study.
RESULTS
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 1.
A, genetic organization of the
N. meningitidis and E. coli K1 capsule loci.
Homologous genes are coded by filled patterns, whereas the
"extra" genes within K1 cps locus are shaded in
gray. B, map of the meningococcal
kpsF locus (NMB0352). In N. meningitidis a 96-bp
intergenic region separates the divergently transcribed tal
and kpsF. Predicted open reading frame (NMB0353) encoding a
hypothetical protein of unknown function is downstream of
kpsF. Restriction sites used in generating insertional
mutations are also labeled.
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Fig. 2.
Protein sequence alignment of the
meningococcal and E. coli KpsF homologues using the
ClustalW method. The sugar isomerase domain is indicated with a
black line above the sequence, and a thick black
bar labeled the location of a predicted Walker A box.
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Fig. 3.
Capsular polysaccharide whole cell ELISAs and
whole cell immuno-dot blots of meningococcal kpsF
mutants. A, surface-expressed capsule of
strains F8239 (serogroup A) and the NMB (serogroup B) and the
corresponding kpsF mutants of these strains were determined
by ELISA. B, serial dilutions of meningococci of
serogroups B, C, Y, and W-135 (from left to
right: 1 × 107, 5 × 106,
1 × 106, and 5 × 105 colony-forming
units/ml) spotted onto nitrocellulose membranes and immunoblotted with
corresponding capsule-specific antibodies.
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Fig. 4.
Silver-stained Tricine SDS-PAGE (16%) of
extracted LOS from the wild type parent NMB (lanes 1,
3, 5, 7, and
9) and the kpsF (NMB206) mutant
(lanes 2, 4, 6,
8, and 10). Labeled molecular weight
markers are on the left. The crude extracts from EDTA/TEA
extraction before (lanes 7 and 8) and after
(lanes 9 and 10) proteinase K treatment are shown
for comparison.
H]
ion of major intensity was m/z 1633, and
those of minor intensities were m/z 1756, 1451, 1435, and
864. The m/z 1756 ion was not present in the spectrum shown
in Fig. 5 but did occur in a second preparation as a minor ion together
with the other ions mentioned. These different molecular ions were due
to variations in phosphate, phosphoethanolamine, and fatty acyl
substitution patterns. Except for m/z 864, all of the
molecular species observed were consistent with the conclusion that the
LOS consisted only lipid A and did not contain any detectable Kdo or
core glycosyl residues. The minor ion at 864 was consistent with a
mono-phosphorylated triacylglucosoamine equivalent to one-half of a
lipid A molecule. Mild acid hydrolysis, which would remove glycosidically linked phosphate, did not alter the MALDI-TOF spectrum and indicated that the single phosphate group was most likely not
glycosidically linked and was, therefore, located at the 4'-position. Much of the heterogeneity in the NMB206 LOS was removed by treatment with aqueous HF, which removes all phosphate substituents. MALDI-TOF MS
analysis in the positive mode of the HF-treated LOS (Fig.
5B) revealed a major [M + Na]+ ion at 1576 (the calculated value is 1577) and a minor ion at 1394. The
m/z 1576 ion was consistent with a molecule of composition GlcN2C12:O2OHC12:O2OHC14:O2,
and the ion at m/z 1394 with
GlcN2C12:O1OHC12:O2OHC14:O2. The m/z 1576 ion was derived from the LOS species at
m/z 1756 and 1633. The m/z 1394 ion was derived
LOS m/z 1451 species. Ions derived from the minor LOS
species at m/z 1435 or 864 were not detected.
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Fig. 5.
MALDI-TOF spectra of NMB206 LOS before
(A) and after (B) removal of
phosphate substituents with aqueous HF. The spectrum in
A was collected in the negative mode and that in
B in the positive mode.
-hydroxylaurate
(215, m/z 1361), -hydroxylauryl (199, m/z
1379), laurate (199, m/z 1379), or lauryl (183,
m/z 1394) fatty acyl components; (b) cleavage
between the glycoside bond (m/z 807 and 791); (c)
cleavage of the glycoside ring of the GlcN residue at the C3-C4 and
C1-O5 bonds (m/z 880); and (d) cleavage of the
glycoside ring at the C4-C5 and C1-O5 bonds (m/z 851). The
remaining fragments are due to the loss of -hydroxylaurate, -hydroxylauryl, or laurate from several of the primary fragments. This fragmentation pattern is completely consistent with the typical symmetrically fatty acylated lipid A reported for N. meningitidis.
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Fig. 6.
A tandem MS/MS spectrum of the 1576 ion of
the HF-treated LOS from the kpsF mutant NMB206
(A) and the structure of the major LOS produced by
NMB206 (B).
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Fig. 7.
Complementation of the meningococcal
kpsF mutation by K1 kpsF.
A, Western immunoblot with FLAG tag-specific
monoclonal antibody. B, Western blot with KpsF-specific
polyclonal antiserum. C, silver stain 16% Tricine
SDS-PAGE of proteinase K-digested whole cell lysate. D,
whole cell capsule ELISA. Meningococcal strains are as follows:
lane 1, wild type serogroup B parent strain, NMB;
lane 2, strain NMB206
(kpsF::aphA-3); lane
3, strain NMB240
(Ptac::K1-kpsF) induced
with IPTG; lane 4, strain NMB240/206
(Ptac::K1-kpsF,
kpsF::aphA-3) induced with IPTG;
lane 5, strain NMB240 without IPTG; lane
6, strain NMB240/206 without IPTG; lane 7,
strain 250 (vector control); lane 8, strain NMB250/206
(vector control with kpsF::aphA-3).
Data in D are normalized to the reading of the wild type
strain, and the average values of at least three independent
experiments are shown.
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Fig. 8.
Whole cell capsule ELISA of the wild type
strain NMB and mutants: strain NMB206
(kpsF::aphA-3), strain
NMB249 (kdtA::aphA-3), and
strain NMB259
(kdsB::aphA-3). The
A405 nm reading of the wild type parent was
normalized to 100% (n 
3).
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Fig. 9.
A, Coomassie-stained SDS-PAGE of KpsF
purification. The meningococcal kpsF containing plasmid
pYT225 was transformed into the E. coli expression strain
BLR21(DE3)pLysS and induced with IPTG. Lane 1, induced
whole cell; lane 2, non-induced whole cell; lane
3, total cleared cell lysate; lane 4,
flow-through of nickel-nitrilotriacetic acid column; lane
5, 20 mM imidazole wash; lane 6,
250 mM imidazole elute. The arrow on the
right indicates the position of KpsF protein and the smaller
protein band (*), likely a degradation product of KpsF because it
reacts with antiserum against KpsF. Molecular mass in kDa is
labeled on the left. B, silver-stained 16%
Tricine SDS-PAGE of LOS extracted from the NMB206 mutant exogenously
complemented with arabinose. C, 31P spectra
of the Ara-5-P isomerase reaction starting with either Ara-5-P
(a-c) or ribulose 5-phosphate (d-f) as
substrates. Spectra a-c were taken at
t = 0, t = 54 min, and
t = 250 min, respectively, with only Ara-5-P ( 
= 4.9 ppm) present at t = 0 min (a).
Spectra f, e, and
d were taken at t = 0, t = 180 min, and t = 600 min, respectively, with only
ribulose 5-phosphate ( = 5.3 ppm) present at t = 0 min (f). Note that spectra c and d
represent equilibrium approached from either substrate and that the
equilibrium ratio of Ara-5-P to ribulose 5-phosphate (65:35) is the
same in either case.
DISCUSSION
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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Fig. 10.
Biosynthesis pathway of
CMP-Kdo.
Although kpsF is conserved (98% amino acid identity) in the kps locus of E. coli strains expressing K1 and K5 capsular polysaccharides (50, 51), the role of KpsF in capsule expression of these bacteria has not been firmly established. The expression of region 1 in E. coli is regulated by temperature at the transcriptional level; however, KpsF is not required for thermoregulation (50). A nonpolar kpsF mutation resulted in about 10-fold reduction of capsule translocation to the surface of K1 E. coli (43). Although Kdo is a component of K5 capsules at the reducing end of the polymers (48), KpsF is not required for K5 capsule expression because cloning the K5 capsule gene cluster lacking kpsF into a K12 strain produced a capsule comparable with that of the wild type K5 strain (52). The lack of clear phenotypes in E. coli kpsF mutants is likely due to the presence of gutQ and/or yrbH. The meningococcal genomes, however, have no other homologues of kpsF. We found that E. coli K1 kpsF complements the meningococcal kpsF mutation in both LOS biosynthesis and capsule expression. This suggests that E. coli K1 and K5 and meningococcal kpsF homologues perform analogous functions.
Our data indicate that Kdo is involved in meningococcal capsule expression. We found that inactivation of the arabinose-5-phosphate isomerase, KpsF, reduces expression of both sialic acid (serogroup B, C, Y, and W-135) and non-sialic acid (serogroup A) meningococcal capsules. Thus, Kdo is not likely to be involved in the activation of the different monomeric substrates (e.g. sialic acid and mannosamine) but may function in capsule assembly, such as an acceptor involved in the initiation of capsule polymerization.
Gotschlich et al. (7) characterized the structures of meningococcal capsular polysaccharides from serogroups A, B, and C. The authors reported a common diacylglycerol substitution at the reducing end of capsule polymers, but no Kdo residues were observed. Although K1 and K5 E. coli have an identical organization of region 1 of the kps locus (Fig. 1), Kdo is found as the reducing sugar in the E. coli K5 capsule (48) but thus far not in K1 polymers. Finke et al. (48) hypothesized that the biosynthesis of K5 capsule is initiated by substitution of an undecaprenol phosphate (UP) carrier with Kdo, which then acts as an acceptor for subsequent capsule polymerization. In the case of K1 E. coli, however, UP alone was proposed to be the acceptor in capsule polymer assembly (53). Our data suggest that Kdo-UP may act as a carrier for capsule polymer assembly in meningococci and may be subsequently replaced by the phospholipid substitution, thus removing Kdo from the final assembled capsule polymers. In both meningococcal kpsF and kdsB mutants, capsule expression was significantly reduced but not completely eliminated, suggesting an alternative acceptor other than Kdo-UP.
Re endotoxin, a Kdo2-lipid A structure, has been
widely regarded as the minimal endotoxin structure that supports
viability (54). However, meningococci were shown recently to be viable without any endotoxin (55). We found that a viable meningococcal kpsF mutant can produce fully acylated lipid A without Kdo
glycosylation (Fig. 6). The kpsF mutant expresses primarily
4'-phosphorylated hexa-acyl lipid A, with minor components consisted of
penta-acyl lipid A and triacylglucosamine. Interestingly, the
glycosidic position of lipid A was unphosphorylated in the
kpsF mutant. A negative charge provided by phosphorylation
at this position has been proposed to be essential (10) or when absent
is provided by the reducing sugar of the lipid A backbone,
-D-galacturonic acid, as in Rhizobium
leguminosarum (31) or in Rhodospirillum fulvum, a
galacturonic acid replaces the phosphate group at the glycosidic
position (56). The absence of phosphorylation at the glycosidic
position was also detected in the unglycosylated lipid A species
expressed by the kdtA mutant.2 These
observations suggest that phosphorylation of the glycosidic position is
optional when Kdo glycosylation is absent. The data also suggest that
N. meningitidis can express a lipid A glycosidic phosphatase.
In summary, we have identified that kpsF in meningococci and
its homologues in E. coli encode the arabinose-5-phosphate
isomerases of prokaryotes. The unique phenotype of the kpsF
mutant should allow us to understand better the genetic basis and
biosynthesis of meningococcal LOS and capsule, two critical virulence
factors in meningococcal pathogenesis.
| |
ACKNOWLEDGEMENTS |
|---|
We thank Dr. Eric Vimr for providing E. coli strains EV36 and EV94 and Nigel Saunders for providing the NMB0352 sequence prior to the publication of the MC58 genome. We are grateful to Yoon Kim Miller and Larry Martin for technical assistance and Lane Pucko for administrative assistance.
| |
FOOTNOTES |
|---|
* This work was supported by United States Public Health Service Grants AI-33517 and AI40247 from the National Institutes of Health (to D. S. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: Vertex Pharmaceuticals, Inc., 130 Waverly
St., Cambridge, MA 02139.
To whom correspondence should be addressed:
Division of Infectious Diseases, Dept. of Medicine, Emory
University School of Medicine, 69 Butler St., S.E., Atlanta, GA
30303. Tel.: 404-728-7688; Fax: 404-329-2210; Email:
dstep01@emory.edu.
Published, JBC Papers in Press, April 15, 2002, DOI 10.1074/jbc.M200931200
2 Tzeng, Y. L., Datta, A., Kolli, K., Carlson, R. W., and Stephens, D. S. (2002) J. Bacteriol. 184, 2379-2388, in press.
3 Y.-L. Tzeng and D. S. Stephens, unpublished results.
| |
ABBREVIATIONS |
|---|
The abbreviations used are:
LOS, lipooligosaccharide;
Bis-tris propane, 1,3-bis[tris(hydroxymethyl)methylamino]propane;
Kdo, 3-deoxy-D-manno-octulosonic acid;
TEA, triethylamine;
Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine;
HF, hydrogen fluoride;
MS, mass spectrometry;
GC-MS, gas
chromatography-mass spectrometry;
ELISA, enzyme-linked immunosorbent
assay;
IPTG, isopropyl-1-thio--D-galactopyranoside;
Ara-5-P, arabinose 5-phosphate;
UP, undecaprenol phosphate.
| |
REFERENCES |
|---|
|
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ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES
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