JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M203276200 on May 30, 2002

J. Biol. Chem., Vol. 277, Issue 34, 31220-31227, August 23, 2002
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/34/31220    most recent
M203276200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bouton, C.
Right arrow Articles by Drapier, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bouton, C.
Right arrow Articles by Drapier, J.-C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Recycling of RNA Binding Iron Regulatory Protein 1 into an Aconitase after Nitric Oxide Removal Depends on Mitochondrial ATP*

Cécile Bouton, Marie-Jeanne Chauveau, Sylvie Lazereg, and Jean-Claude DrapierDagger

From the Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France

Received for publication, April 5, 2002, and in revised form, May 23, 2002

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Iron regulatory proteins (IRPs) control iron metabolism by specifically interacting with iron-responsive elements (IREs) on mRNAs. Nitric oxide (NO) converts IRP-1 from a [4Fe-4S] aconitase to a trans-regulatory protein through Fe-S cluster disassembly. Here, we have focused on the fate of IRE binding IRP1 from murine macrophages when NO flux stops. We show that virtually all IRP-1 molecules from NO-producing cells dissociated from IRE and recovered aconitase activity after re-assembling a [4Fe-4S] cluster in vitro. The reverse change in IRP-1 activities also occurred in intact cells no longer exposed to NO and did not require de novo protein synthesis. Likewise, inhibition of mitochondrial aconitase via NO-induced Fe-S cluster disassembly was also reversed independently of protein translation after NO removal. Our results provide the first evidence of Fe-S cluster repair of NO-modified aconitases in mammalian cells. Moreover, we show that reverse change in IRP-1 activities and repair of mitochondrial aconitase activity depended on energized mitochondria. Finally, we demonstrate that IRP-1 activation by NO was accompanied by both a drastic decrease in ferritin levels and an increase in transferrin receptor mRNA levels. However, although ferritin expression was recovered upon IRP-1-IRE dissociation, expression of transferrin receptor mRNA continued to rise for several hours after stopping NO flux.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In the early nineties, the notion that iron-sulfur cluster proteins may play an important role in regulating gene expression began to emerge (1). Iron regulatory protein 1 (IRP-1),1 which controls intracellular iron homeostasis at a post-transcriptional level, is one of the best illustrations. Once properly activated, IRP-1 specifically binds to one or several iron-responsive element(s) (IRE) located in the untranslated regions (UTRs) of several mRNAs, including those encoding ferritin, mitochondrial aconitase, and transferrin receptor (Tf-R). The binding of IRP-1 to IRE in the 5'-UTR of ferritin or mitochondrial aconitase mRNA blocks its translation, whereas binding of IRP-1 to at least three of the five IREs in the 3'-UTR of Tf-R mRNA stabilizes it. Remarkably, IRP-1 is a bifunctional protein that in resting cells displays a [4Fe-4S] cluster at the active site and consequently exhibits an aconitase activity in the cytosol. When cellular iron becomes limiting, cytosolic aconitase activity disappears, whereas IRP-1 progressively gains the capacity to bind IREs. A second IRP, named IRP-2, also binds to IRE motifs in response to iron depletion but does not have aconitase activity because of its incapacity to assemble an Fe-S cluster (for review, see Ref. 2).

It is now well known that nitric oxide (NO) can also modulate IRP-1 (3, 4). Moreover, several lines of evidence indicate that activation of IRP-1 by NO is followed by repression of ferritin translation and an increase in Tf-R mRNA levels (4, 5). In contrast to iron depletion, NO quickly modulates IRP-1 activity at a post-translational level (6, 7). NO rapidly converts IRP-1 from aconitase to its trans-regulatory form without requiring de novo protein synthesis (6). Its primary target site is the Fe-S cluster, because it has no effect on IRP-1 from cytosol that had been pretreated with an excess of aconitase substrates (e.g. citrate or cis-aconitate) known to interact directly with the labile iron of the cluster (8, 9). Subsequently, electron paramagnetic resonance studies have definitively established that NO promotes the cluster disassembly of the protein (10). At this juncture, one of the challenging issues ahead was to investigate the fate of the trans-regulatory form of IRP-1 once NO flux stops. This question addresses the still ill-defined cell machinery involved in iron-sulfur cluster (ISC) assembly in mammals. Recent results using yeast indicate that mitochondria are most probably the primary site of the biogenesis of both mitochondrial and cytosolic Fe-S cluster proteins (11-13). Yet in mammalian cells, components of the ISC machinery have recently been characterized in extra-mitochondrial compartments (14, 15). Taking these recent data into consideration and to understand better the impact of NO on proteins whose mRNAs are under the control of the IRP/IRE system, we have investigated the possible Fe-S cluster repair of NO-modified IRP-1 when NO flux stops. In parallel, we have analyzed the consequences of this regulation for ferritin and mitochondrial aconitase expression as well as Tf-R mRNA level. In addition to IRP-1, we have also explored the fate of the related mitochondrial Fe-S aconitase when cells were no longer exposed to NO and studied the participation of energized mitochondria in the recovery of their aconitase activity.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Materials-- Spermine NONOate (SPER/NO), DPTA NONOate (DPTA/NO), and DETA NONOate (DETA/NO) were from Cayman Chemical (Ann Arbor, MI). Cycloheximide (CHX), carbonyl cyanide m-chlorophenylhydrazone (CCCP), LD-L 10 kit, and oligomycin were from Sigma.

Cell Culture and Treatments-- The RAW 264.7 macrophage cell line was cultured in high glucose Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum (Invitrogen). Cells were incubated with different NONOates for the times indicated in the figures and washed twice with phosphate-buffered saline. Cells were then incubated in fresh medium alone or with medium that contained CHX or CCCP as indicated in the figure legends. At the indicated times, cell were harvested, and both cytosol and mitochondria-enriched fractions were prepared. To determine the expression of Tf-R mRNA, total RNAs were extracted in parallel experiments.

Preparation of Mitochondria-enriched Fraction-- RAW 264.7 cells (5 × 106/ml) were harvested and suspended in 0.25 M sucrose buffered by 100 mM HEPES, pH 7.4. They were then treated with 0.007% digitonin as described (16). The mitochondria-enriched pellet was treated with a lysis buffer composed of 100 mM Tris, pH 7.5, 0.5% Triton X-100, and 20 µg/ml phenylmethylsulfonyl fluoride. After 20 min on ice, the lysate was centrifuged at 10,000 × g for 10 min at 4 °C, and the supernatant was immediately tested for mitochondrial aconitase activity and aliquots were kept at -80 °C for Western blot analysis. Cytosolic contamination of mitochondria-enriched fractions was determined by measuring the amount of lactate dehydrogenase activity associated with the mitochondria-enriched fraction (kit LD-L 10, Sigma Diagnostics). This amount value did not exceed 5%. In control experiments, we also checked by Western blot that IRP-1 protein was not detectable in mitochondria-enriched fractions.

Preparation of Cytoplasmic Extracts-- RAW 264.7 cell monolayer (5 × 106/ml) was harvested in 250 µl of 0.25 M sucrose buffered with 100 mM HEPES, pH 7.4. The cell suspension was then treated with 0.007% digitonin, and the lysate was centrifuged at 1,800 × g for 10 min. The resulting supernatant was then centrifuged at 100,000 × g for 1 h at 4 °C. The cytosolic extract was tested for protein concentration (~1 mg/ml) before measuring aconitase activity. No mitochondrial aconitase expression was detected by Western blotting in the cytosolic fraction. Samples were then kept at -80 °C for further measurements.

Preparation of RNA Transcripts-- Transcription reactions were performed in vitro with 1 or 2 µg of either linearized pSPT-TR 11 (corresponding to the 2.3-kilobase 3'-UTR of the human Tf-R) or linearized pSPT-fer (corresponding to the human ferritin H-chain IRE) (kindly provided by Dr. L. C. Kühn) in the presence of 60 µCi of [32P]CTP, 2.5 mM unlabeled NTP, 0.02 mM unlabeled CTP, 10 mM DTT, 20 units of RNase inhibitor, and 20 units of T7 RNA polymerase in a final volume of 20 µl. Samples were incubated for 2 h at 38 °C. Full-length transcripts from pSPT-TR11 were purified on Sephadex G50 column.

Electromobility Shift Assay-- RNA-protein interactions were performed as described previously (17). Briefly, 2 µg of cytoplasmic extracts were mixed with a molar excess of labeled H-chain ferritin IRE in a final volume of 20 µl of 10 mM HEPES, pH 7.6, 40 mM KCl, 3 mM MgCl2, and 5% glycerol (buffer A). After 20 min of incubation at room temperature, 1 µl of RNase T1 (1 unit/µl) was added. After 10 min, 2 µl of 50 mg/ml heparin were added for an equivalent additional time. IRP-IRE complexes were resolved in 6% nondenaturing polyacrylamide gels. In a parallel experiment, samples were treated with 2% 2-mercaptoethanol (2-ME) before the addition of the labeled RNA probe. This treatment allows full expression of IRP-1 IRE binding activity. The IRP-1-IRE complex was quantified with ImageQuant software (Molecular Dynamics). RNA-protein interactions were also analyzed with a molar excess of labeled 3'-UTR of the Tf-R, which contains 5 IRE sequences (TfR 5-IREs). The above-mentioned protocol was slightly modified for the Tf-R probe; 10 µg of cytoplasmic extracts were used in a final volume of 30 µl of buffer A. Incubation time for the Tf-R 3'-UTR-IRPs interactions was 40 min. Four units of RNase T1 were then added for 20 min followed by 3 µl of 50 mg/ml heparin for 10 min.

Western Blot Analysis-- Ten micrograms of protein from cytosolic extracts or 0.1 µg of purified mouse liver ferritin were resolved on 12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes. The membranes were then blocked with gentle agitation in 20 mM Tris-base, pH 7.5, 137 mM NaCl, 0.1% Tween 20 buffer containing 5% fat-free dry milk at 37 °C for 1 h followed by an overnight incubation at 4 °C with a purified mouse liver ferritin antiserum (kindly provided by Dr. J. Brock, Glasgow University, Glasgow, UK). Membranes were then washed and incubated with a peroxidase-conjugated goat anti-rabbit secondary antibody (Amersham Biosciences) for 1 h at room temperature. The immunoreactive bands were detected by using the enhanced chemiluminescence Western blotting detection system Super Signal (Pierce). To analyze the expression of mitochondrial aconitase, we used an antibody raised against purified beef heart mitochondrial aconitase and kindly provided by Dr. R. B. Franklin, University of Maryland, Baltimore, MD. Concerning analysis of IRP-1 expression levels, we used a chicken polyclonal antibody raised against purified human recombinant IRP-1 (Agro-Bio, La Ferté Saint-Aubin, France).

Northern Blot Analysis-- Total RNA from RAW 264.7 macrophages was extracted using the TRIzolTM reagent (Invitrogen). Equal amounts of RNA (20 µg) were electrophoresed at 61 mV for 2-3 h in a 1% agarose/formaldehyde gel and transferred to a positively charged nylon membrane (Pall Corp. Ann Arbor, MI). After cross-linking of the RNA to the membrane by UV light and prehybridization for at least 1 h with Hybrizol I solution (Intergen Co., NY), membranes were probed overnight with the 2.3-kilobase EcoRI fragment of the murine Tf-R cDNA (kindly provided by Dr. L. C. Kühn, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland), which was labeled by the random hexamer priming method (Invitrogen). Blots were washed twice in 2× standard saline phosphate/EDTA (0.15 M NaCl/10 mM phosphate, pH 7.4, 1 mM EDTA) for 15 min at room temperature and then twice in 0.1× standard saline phosphate/EDTA at 55 °C for 15 min before exposing to phosphorimaging. Blots were also re-probed with a 1.3-kilobase PstI fragment of glyceraldehyde-3-phosphate dehydrogenase cDNA as a loading control and washed as above except for the last two washes, which were performed at 65 °C.

Aconitase Activity-- Mitochondrial and cytosolic aconitase activities were determined spectrophotometrically at 240 nm by following the disappearance of cis-aconitate, which is the intermediate substrate of aconitases, at 37 °C. Fifty micrograms of protein were used in 100 mM Tris-HCl, pH 7.4. Units correspond to nmol of substrate consumed/min.

In Vitro Iron-Sulfur Cluster Reconstitution-- Reconstitution of the [4Fe-4S] cluster in IRP-1 from control or IFN-gamma /LPS-treated cell cytosols was carried out by the addition of 10 mM DTT, 125 µM ferrous ammonium sulfate, and 125 µM sodium sulfide to 100 µg of protein extracts in 20 mM HEPES, pH 7.5, 20 mM KCl, and 5% glycerol. All reactions were performed under an anaerobic atmosphere for 20 min.

Oxygen Consumption-- Oxygen consumption was measured with a Clark-type oxygen electrode from Hansatech (Norfolk, UK). RAW 264.7 cells were cultured in high glucose Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum at 37 °C in 5% CO2 humidified atmosphere and treated with the uncoupler of oxidative phosphorylation CCCP for 6 h. Control or CCCP-treated cells (10 × 106/ml) were harvested, washed twice with the respiration buffer (25 mM HEPES, pH 7.2, 20 mM sucrose, 1.2 mM KH2PO4, 118 mM NaCl, 4.8 mM KCl, 1 mM CaCl2), and then centrifuged at 232 × g for 10 min. The cell pellet was resuspended in 1 ml of pre-warmed respiration buffer and homogenized with constant stirring in the respiration chamber at 37 °C during measurements. During the oxygen consumption measurement, the mitochondrial ATPase inhibitor oligomycin (10 µM) was added to the chamber.

Measurement of Protein Synthesis-- Cells were cultivated with various concentrations of CHX (250, 500, and 1000 ng/ml) in leucine-starved Dulbecco's modified Eagle's medium for 30 min. Then cells were pulse-labeled with 2 µCi/ml of L-[3H]leucine for 2 h. After incorporation of leucine, cells were washed 10 times and harvested in a solution of 10% trichloroacetic acid. Samples were filtered under a vacuum through a Whatman GF/B filter, and 100 µl of the filtrate were used for measuring incorporated 3H radioactivity as described previously (18). Cell viability was estimated by measuring lactate dehydrogenase release using the LD-L 10 kit. We chose the concentration of 1 µg/ml CHX, which completely blocked translation without cell toxicity.

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

In Vitro Reconstitution of the [4Fe-4S] Cluster into IRE Binding IRP-1 from NO-producing Macrophages-- We first investigated the possible in vitro repair of the [4Fe-4S] cluster in IRE binding IRP-1 from RAW macrophages previously stimulated by IFN-gamma /LPS to produce NO. Nitrite production was verified by nitrite accumulation in the culture medium (data not shown). Cytosols from control or NO-producing cells were extracted and incubated with a source of ferrous iron and sulfide in the presence of DTT. After 20 min under an anaerobic atmosphere, cytosols were tested for their IRP-1-IRE binding and aconitase activities. In the IRP-1-IRE binding assay, samples were routinely treated with 2% 2-ME in a parallel experiment to express full IRE binding activity (Fig. 1A, lower panel). Untreated cytosols from control cells presented low IRP-1-IRE binding activity and significant aconitase activity (27.9 units/mg) (Fig. 1A, lane 1, and B). The addition of iron, sulfide, and DTT further attenuated IRP-1-IRE binding activity and increased aconitase activity to 41.1 units/mg (Fig. 1A, lane 2, and B). In contrast, cytosols from NO-producing cells showed full IRP-1-IRE binding activity and no aconitase activity (Fig. 1A, lane 3, and B). Interestingly, we observed that treatment of these cytosols with iron, sulfide, and DTT almost completely abolished IRP-1 affinity toward IRE (Fig. 1A, compare lane 4 to lane 3). This drastic decrease in IRE binding was not observed if one of the components used for the Fe-S cluster reconstitution was missing (data not shown). In parallel to the loss of IRE binding by IRP-1, cytosolic aconitase activity resumed and reached up to 50% that of control cytosols (Fig. 1B, black bars). Based on recent data showing down-regulation of IRP-1 gene expression by NO (19), we measured the IRP-1 expression level under our experimental conditions. In fact, the IRP-1 protein level was decreased by 57% in NO-producing cells (Fig. 1C), explaining why only half of aconitase activity was recovered. These data show that virtually every IRP-1 from NO-producing cells had their Fe-S cluster repaired. We also showed that in vitro repaired Fe-S clusters were stable for at least 2 h in air, as indicated by the constant level of aconitase activity (data not shown).


View larger version (17K):
[in this window]
[in a new window]
  		Fig. 1.   In vitro reconstitution of the [4Fe-4S] cluster in IRP-1 from IFN-gamma /LPS-treated RAW 264.7 macrophages. Cells were exposed to 20 units/ml IFN-gamma and 50 ng/ml LPS for 16 h. Cytosols from control or IFN-gamma /LPS-treated cells were then extracted. A and B, reconstitution of the [4Fe-4S] cluster in IRP-1 was carried out by the addition of 125 µM ferrous ammonium sulfate (Fe2+), 125 µM sodium sulfide (S2-), and 10 mM DTT to 100 µg of control or IFN-gamma /LPS-treated cell cytosol in 300 µl of 20 mM HEPES, pH 7.5, 5% glycerol, and 20 mM KCl. All reactions were performed at room temperature under anaerobic atmosphere for 20 min. Two µg of protein of each reaction were then analyzed for IRP-1-IRE binding activity (panel A) as described under "Experimental Procedures." In panel B, IRP-1-IRE binding activity was quantified by phosphorimaging and expressed as percent of the value obtained with 2% 2-ME (open bars). In parallel, 50 µg of protein were transferred to an open-top cuvette for UV spectroscopy to measure aconitase activity (filled bars). C, IRP-1 levels were analyzed by Western blotting as described under "Experimental Procedures" and quantified by densitometry. Purified recombinant IRP-1 (rIRP-1) was used as the positive control. The experiments were performed four times, and a representative result is shown.

Change in IRP-1 Activities after Stopping Exposure of Cells to Exogenous NO-- To investigate the change in IRP-1 structure/activities in intact cells when NO flux stops, we used NONOates because of their predictable and various rates of NO release. To activate IRP-1, we first exposed cells to three different NONOates, SPER/NO, DPTA/NO, and DETA/NO. Under our experimental conditions, they released NO linearly at different rates, 3-4, 1.5, and 0.4 µM/min, respectively. We showed that the fast NO-releasing SPER/NO led to a rapid activation of IRP-1 within 2 h (Fig. 2A, upper row, compare lane 6 to lane 1). Aconitase activity was strongly decreased, corresponding to 38% that of the control (right panel of A). DPTA/NO triggered significant activation of IRP-1 within 4 h (Fig. 2B, compare lane 5 to lane 1) with a remaining aconitase activity of 22% of the control (right panel of B). Finally, DETA/NO, whose rate of NO release is similar to that of inducible NO synthase (20), allowed strong activation of IRP-1 within 16 h (Fig. 2C, compare lane 4 to lane 1) and showed a remaining aconitase activity of 19% of the control (right panel of C). After removing NO donors by extensive washing, we measured both aconitase and IRE binding activities of IRP-1 at the indicated times. IRP-1-IRE binding activity progressively decreased (Fig. 2, left panels) while the cells were recovering cytosolic aconitase activity (Fig. 2, right panels). This reverse change in IRP-1 was apparent within the first hours after NO removal whatever the rate of NO release previously generated to activate IRP-1. NO also inhibits mitochondrial aconitase through disruption of its [4Fe-4S] cluster (10, 21). Therefore, we also tested the possible restoration of its activity under the same experimental conditions. Mitochondrial aconitase from NO-treated cells was quickly recovered after stopping NO flux (data not shown).


View larger version (30K):
[in this window]
[in a new window]
  		Fig. 2.   Simultaneous decrease in IRP-1 IRE binding activity and rise in cytosolic aconitase activity after NO donor removal. RAW 264.7 cells were incubated with 0.2 mM SPER/NO for 2 h (A), 0.4 mM DPTA/NO for 4 h (B), and 0.5 mM DETA/NO for 16 h (C). Cells were then washed to remove the NO donor and further incubated with fresh medium. At the indicated times, cytosols were extracted and analyzed for IRP-1-IRE binding activity (left panels) and aconitase activity (right panels) as described in Fig. 1. Aconitase activity was expressed as a percent of the appropriate control value (%/control). The experiments were performed at least three times, and a representative result is shown. Ft, ferritin.

To investigate whether resumption of IRP-1 and mitochondrial aconitase activities requires de novo protein synthesis, CHX was added to cell culture medium. As previously shown, a 16-h preincubation of macrophages with DETA/NO was accompanied by loss of cytosolic aconitase activity (5% of control, left panel of Fig. 3A) and a concomitant increase in IRE binding capacity of IRP-1 (Fig. 3B, compare lane 2 to lane 1). The addition of CHX after washing away DETA/NO did not preclude the reappearance of cytosolic aconitase activity, which reached the same level as CHX-untreated cells (Fig. 3A, left panel). Interestingly, IRE binding capacity of IRP-1 was more strongly decreased when protein synthesis was blocked (Fig. 3B, compare lane 6 to lane 4). Similar experiments using DPTA/NO as a source of NO gave the same results (data not shown). The latter experiments demonstrate that reversion of IRP-1 activities after shutting off NO flux was not due to newly synthesized IRP-1 but was dependent on a post-translational change of the NO-modified IRP-1 itself. We also observed a rapid increase in mitochondrial aconitase activity, reaching up to 70% of the control value 6 h after DETA/NO removal even in the presence of CHX (Fig. 3, right panel of A).


View larger version (24K):
[in this window]
[in a new window]
  		Fig. 3.   Effect of cycloheximide on change in IRP-1 and mitochondrial aconitase activities after DETA/NO removal. A, cells were exposed to 0.5 mM DETA/NO for 16 h. After extensive washing, cells were further incubated with fresh medium or medium containing 1 µg/ml CHX. After 6 h, cytosols and mitochondria-enriched fractions were extracted as described under "Experimental Procedures" and tested for their respective aconitase activity. Aconitase activity was expressed as a percent of the appropriate control value (%/control) (panel A). In a parallel experiment, cytosols were also analyzed for IRP-1-IRE binding activity by electromobility shift assay (upper panel of B). IRP-1-IRE binding activity was quantified by phosphorimaging and expressed as a percent of the value obtained with 2% 2-ME (lower panel of B). The experiments were performed three times, and a representative result is shown.

Effect of Mitochondria De-energization on IRP-1 and Mitochondrial Aconitase Activity Repair-- Biosynthesis of extra-mitochondrial Fe-S cluster proteins in yeast has been found to depend on an ATP binding cassette (ABC) transporter located in the inner membrane of mitochondria (22). Accordingly, we analyzed whether the reverse change in IRP-1 activities as well as the activity repair of the mitochondrial aconitase after DETA/NO removal required an ATP-dependent mechanism from mitochondria. As described above, DETA/NO was removed, and the cells were then treated with an uncoupler of oxidative phosphorylation, CCCP. In a preliminary experiment, we showed that it instantaneously de-energized mitochondria. We then performed a time-course experiment to follow the activity of both IRP-1 and mitochondrial aconitase. We showed that recovery of aconitase activity was markedly delayed in the cytosol of CCCP-treated cells as compared with untreated cells (left panel of Fig. 4A, compare the white bars to the black bars). Conversely, the high IRE binding activity of IRP-1 resulting from the treatment by DETA/NO remained elevated in CCCP-treated cells at least 6 h after stopping NO flux (data not shown). Recovery of mitochondrial aconitase activity was also delayed in CCCP-treated cells but to a lesser extent (right panel of Fig. 4A, compare white bars to black bars). In parallel experiments, the uncoupler CCCP was tested for its efficiency on mitochondria de-energization (Fig. 4B). Results show that CCCP was effective during the a 6-h recovery phase, as indicated by the unchanged oxygen consumption of CCCP-treated cells after inhibition of ATP synthesis by oligomycin.


View larger version (18K):
[in this window]
[in a new window]
  		Fig. 4.   Effect of the uncoupler of oxidative phosphorylation CCCP on the restitution of cytosolic and mitochondrial aconitase activities after DETA/NO removal. A, RAW 264.7 macrophages were pretreated with DETA/NO for 16 h. Cells were then washed to remove the NO donor and incubated with fresh medium or medium containing 10 µM CCCP for additional indicated times. A, cytosols and mitochondria-enriched fractions were then extracted and tested for their respective aconitase activity. Aconitase activity was expressed as a percent of control value (%/control). B, cells (10 × 106/ml) were treated with 10 µM CCCP for 6 h, and then oxygen consumption was tested as described under "Experimental Procedures." As indicated by an arrow, the mitochondrial ATPase inhibitor oligomycin (10 µM) was added to the chamber. The experiments were performed three times, and a representative result is shown.

Recovery of Initial IRP-1 Activities Correlates with Resumption of High Levels of Ferritin-- We investigated whether inactivation of IRP-1 trans-regulatory activity was followed by a change in expression of proteins whose mRNA contains an IRE sequence in their 5'-UTR. We first incubated RAW macrophages with DETA/NO for 16 h and analyzed their cytosols for aconitase activity and IRE binding by IRPs using the ferritin IRE as a probe. In parallel, we measured the ferritin expression level. We showed that both down-regulation of IRP-2-IRE binding and activation of IRP-1-IRE binding in DETA/NO-treated cells resulted in severe reduction of ferritin expression (Fig. 5). Six hours after NO donor removal, loss of IRP-1 in its capacity to bind ferritin IRE was directly correlated with resumption of ferritin translation (Fig. 5C, compare lane 4 to lane 2). Noteworthy, loss of IRE binding activity of IRP-2 in response to NO was not reversed after stopping NO flux. In parallel, the level of mitochondrial aconitase, whose mRNA also has an IRE in its 5'-UTR (23, 24), was measured. Despite activation of IRP-1 to bind IRE, the level of this protein was not reduced in response to an overnight stimulation by NO and remained unchanged 6 h after stopping NO flux once IRP-1 was inactivated (Fig. 5C, lower panel).


View larger version (20K):
[in this window]
[in a new window]
  		Fig. 5.   Effect of IRP-1 deactivation on ferritin and mitochondrial aconitase levels. Cells were pretreated with 0.5 mM DETA/NO for 16 h. Cytosols from control (lane 1) or DETA/NO-treated cells (lane 2) were then extracted. In a parallel experiment, the medium of control (lane 3) or DETA/NO-pretreated cells (lane 4) was replaced with fresh medium for an additional 6 h before extracting cytosols. A, analysis of IRP-1 and IRP-2-IRE binding activity using the ferritin IRE probe (IRE-Ft). B, measurement of cytosolic aconitase activity. The enzymatic activity is expressed as a percent of the appropriate control value (%/control). C, expression of cytosolic ferritin and mitochondrial aconitase (mt-aco) levels. Equal amounts of protein (10 µg) were subjected to Western blot analysis using an anti-ferritin and anti-mitochondrial aconitase antisera. Purified mouse liver ferritin and mitochondrial aconitase (Sigma) were used as positive control (lanes 5). The experiments were performed four times, and a representative result is shown.

Resumption of IRP1 Activities and Consequences for Tf-R mRNA Level-- Tf-R mRNA contains five IRE sequences in its 3'-UTR (25). We therefore studied the regulation of its expression after the change in IRP-1 activities induced by the "on/off NO flux signal" described above. It is worth noting that in this set of experiments, IRP-1 and IRP-2-IRE binding capacity was assessed using the entire 3'-UTR that contained the five IRE sequences of the Tf-R as a probe. In parallel to cytosolic extraction, total mRNAs were extracted and analyzed for Tf-R expression. We first observed that the capacity of IRP-2 to bind Tf-R IREs was not significantly modified in control and DETA/NO-exposed cells (Fig. 6A, compare lane 2 to lane 1). In contrast, we observed that exposure of macrophages to DETA/NO resulted in a strong increase in IRP-1 binding capacity toward Tf-R IRE sequences that was accompanied by an enhanced Tf-R mRNA expression (Fig. 6, panel A and C). After 6 additional hours without DETA/NO, IRP-1 no longer interacted with Tf-R IREs and recovered aconitase activity (Fig. 6A, compare lane 4 to lane 2, and panel B), but surprisingly, this was not accompanied by a decrease in Tf-R mRNA level. Actually, Tf-R mRNA expression continued to increase even 10 h after exposure to NO was ended (data not shown).


View larger version (20K):
[in this window]
[in a new window]
  		Fig. 6.   Effect of IRP-1 deactivation on Tf-R mRNA expression. Cells were pretreated with 0.5 mM DETA/NO for 16 h. Control (lane 1) and DETA/NO-pretreated cell cytosols (lane 2) were then extracted. In a parallel experiment, the medium of control cells (lane 3) and DETA/NO-pretreated cells (lane 4) was replaced with fresh medium for an additional 6 h before extracting cytosols. A, analysis of IRP-1 and IRP-2-IRE binding activity using the entire 3'-UTR of Tf-R mRNA as probe (TfR 5-IREs). B, cytosolic aconitase activity was measured spectrophotometrically at 240 nm as described under "Experimental Procedures." C, in a parallel experiment, cells were treated as in panel A, and total mRNA was extracted. Twenty µg of total mRNA extracts were probed with the 2.3-kilobase fragment of the Tf-R cDNA (upper panel of C). The membranes were rehybridized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe (lower panel of C). The experiments were performed four times, and a representative result is shown.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Whereas the mechanism of IRP-1 activation in response to NO has been progressively disclosed, the inactivation process of IRP-1 when cells are no longer exposed to NO has never been characterized. Investigating this pathway is crucial to understanding the real impact of NO on proteins whose mRNAs contain the IRE motif(s). In this report, we first showed that in addition to iron and DTT, sulfide was required to ensure both IRP-1-IRE dissociation and recovery of aconitase activity. As documented earlier by Kennedy et al. (26), this observation strongly suggests that NO-modified IRP-1 from activated macrophages is an apoprotein that can be readily reactivated through repair and reinsertion of its [4Fe-4S] cluster. Interestingly, we have observed that recovery of IRP-1 aconitase activity also occurred in intact cells as soon as they were relieved of the NO burden, whatever the time course and the rate of NO formerly generated. Because recovery did not require de novo protein synthesis, we propose that IRE binding IRP-1 could be recycled into an aconitase through reassembly and reinsertion of the Fe-S cluster into the backbone of the protein upon NO removal (Fig. 7). Along the same lines, we noticed that the presence of a labile protein(s) may be necessary to keep the IRP-1-IRE interaction, because the translation inhibitor cycloheximide favored the IRP-1-IRE dissociation in cells relieved of NO (Fig. 3B). Taking into account this result, we are now seeking new partner proteins and/or chaperones that could participate in the IRP-1 activation/deactivation process in response to the on/off NO flux signal. We also investigated the repair activity of the related mitochondrial aconitase after stopping NO exposure. This enzyme, whose Fe-S cluster and in turn enzymatic activity are altered by NO (10, 18, 21), regained its function easily as soon as NO flux stopped without requiring protein translation. Again, these in vivo experiments point to a reinsertion of a fully assembled [4Fe-4S] cluster into NO-modified mitochondrial aconitase. Altogether, our results also indicate that cellular factors that participate in aconitase recovery were left intact during NO exposure and were therefore available to reconstitute IRP-1 and mitochondrial aconitase initial functions once NO flux stopped.


View larger version (23K):
[in this window]
[in a new window]
  		Fig. 7.   Schematic representation of the NO/IRP-1/ferritin connection. In the cytosol of resting cells, a fully assembled [4Fe-4S] cluster allows IRP-1 to function as an aconitase. 1, upon NO exposure, the Fe-S cluster is disassembled, and a relaxed form of the protein binds ferritin IRE, most likely with the participation of a labile accessory protein(s). As a consequence, ferritin translation is repressed. Upon NO removal, IRP1 dissociates from IRE (2) and, via a mitochondrial ATP-dependent process (3), recovers cytosolic aconitase activity, which implies [4Fe-4S] cluster reconstitution (4). As a result, a high level of ferritin is rapidly restored (5).

It is now clear that Fe-S cluster formation and repair do not occur spontaneously in vivo. In yeast and bacteria, the ISC (iron-sulfur cluster assembly) machinery, which comprises some 10 specific proteins, is responsible for both iron and elemental sulfur delivery and (re)assembly of Fe-S clusters (11). Interestingly, it was recently reported that the ISC machinery, in particular the NifS-like IscS protein, could efficiently repair the NO-modified ferredoxin [2Fe-2S] cluster in Escherichia coli (27). It is, thus, reasonable to assume that the recently identified mammalian NifS-like proteins (14, 15, 28) may participate in the Fe-S cluster repair of NO-modified IRP-1 and mitochondrial aconitase. As previously mentioned, Kispal et al. (22) also found that maturation of cytosolic Fe-S cluster-containing proteins of Saccharomyces cerevisiae depends on an ABC transporter, named Atm1p, located in the inner membrane of mitochondria. It has been reported that this transporter exports Fe-S clusters previously constituted in the matrix of mitochondria to the cytosolic compartment. Importantly, a mitochondrial ABC transporter named ABC7 has also been identified in mouse and human as the functional orthologue of yeast Atm1p (29-32). In humans, mutation of the ABC7 gene causes X-linked sideroblastic anemia with cerebellar ataxia. The ABC7 ATP binding domain faces the mitochondrial matrix. It is therefore relevant to investigate the role of energized-mitochondria in the Fe-S cluster repair of NO-modified aconitases. We found that recovery of initial IRP1 activities was markedly delayed in cells whose mitochondria were unable to produce ATP. These data provide the first evidence that mitochondrial ATP production is crucial for Fe-S cluster repair of an extra-mitochondrial protein in mammalian cells. Resumption of mitochondrial aconitase activity was also affected upon uncoupling, albeit to a lesser extent than that of IRP-1, suggesting that it also relies on an ATP-dependent mechanism. We therefore hypothesize that mitochondrial ABC7 is involved in IRP1 Fe-S cluster repair, but alternative mechanisms cannot be ruled out. In bacteria and yeast, hsp70 chaperones such as Jac1p and Ssq1p, whose activity depends on ATP, are involved in the formation and repair of Fe-S clusters (33). It is therefore possible that their putative mammalian homologs are required for Fe-S cluster repair of both mitochondrial aconitase and cytosolic IRP-1. Moreover, we also noticed that IRP-1 cannot dissociate from IRE if mitochondria are unable to produce ATP. This result supports the idea that IRP-1-IRE complex dissociation, an obligate step upstream of Fe-S cluster repair, requires an ATP-dependent mechanism not involving the ISC machinery. This proposal is reminiscent of the ATP-dependent protein-DNA dissociating activity of the yeast protein Mot1 (34).

To understand better the role of NO in iron and energy metabolisms, we then analyzed whether the expression of ferritin and mitochondrial aconitase, whose mRNAs contain an IRE motif, matched the IRP-1 activation/deactivation process induced by the on/off NO flux signal. Interestingly, we noticed that DETA/NO mimicked the effect of stimulation by IFN-gamma /LPS on macrophage IRP-1 and IRP-2 activities (6, 35, 36). Therefore, use of DETA/NO by avoiding interference due to the immunological stimuli proved a relevant approach to examining the real impact of both IRPs on the control of iron metabolism in response to NO. We showed that IRP-1 activation combined with concomitant IRP-2 inactivation was accompanied by a complete inhibition of ferritin expression. This points to a determinant role of IRP-1 in this regulation versus IRP-2. Moreover, in macrophages re-incubated in fresh culture medium after 16 h of exposure to DETA/NO, the inability of IRP-1 to bind the ferritin IRE motif perfectly correlated with reappearance of ferritin expression as illustrated in Fig. 7. Because inhibition of ferritin translation increases sensitivity to oxidative stress (37-38), it is conceivable that the NO-dependent modulation of IRPs strengthens the macrophage array of weapons against intracellular pathogens.

In contrast to ferritin and despite a functional IRE in its mRNA (24, 39), the level of mitochondrial aconitase was not modulated in response to overnight exposure to DETA/NO. This could be explained by the fact that mitochondrial aconitase is a very stable protein with a half-life >16 h in RAW 264.7 cells (data not shown). Therefore, regulation of mitochondrial aconitase via the IRP/IRE system, if any, could be visible only after several days, in line with what has been described in mice maintained for several weeks on a low iron diet (39).

Lastly, to investigate whether IRP-1 activation/deactivation is correlated with regulation of Tf-R expression level, we analyzed changes in IRP activities in macrophages exposed to our experimental on/off NO flux signal. As expected, IRP-1-IRE binding greatly increased in response to NO, but surprisingly, binding of IRP-2 to Tf-R-specific IREs was unchanged. This exclusive IRP-1 activation was accompanied by a noticeable increase in Tf-R mRNA levels. Strikingly, the level of this mRNA continued to increase even after 10 h of cell culture in the absence of NO and despite the fact that IRP-1 had long been dissociated from IREs and had recovered an aconitase activity. This unexpected high expression of Tf-R mRNA indicates that it is not solely controlled post-transcriptionally by IRPs. This NO-dependent steady up-regulation requires additional mechanisms that deserve further investigation, but some clues are already appealing. Recently, it has been shown that NO induces accumulation of the transcription factor hypoxia-inducible factor 1 (40). Assuming that a hypoxia response element, the binding site for hypoxia-inducible factor 1, has been identified in the Tf-R promoter region (41), the continuous increase in Tf-R mRNA could be explained by a long-lasting activation of hypoxia-inducible factor 1 by NO. However, preliminary studies using transcription inhibitors suggest that Tf-R mRNA remains stable after DETA/NO removal.2 It is, thus, tempting to speculate that NO may interfere with the machinery involved in the degradation of Tf-R mRNA. Unfortunately, this process is still largely unknown, and to date only polymerase III transcripts have been suspected to be involved (42).

In conclusion, our results demonstrate that fluctuation of NO levels regulates IRP-1 by inducing reversible post-translational modifications that allow the same molecule of IRP-1 to commute quickly between two activities. One function, aconitase, is lost, but another, IRE binding capacity, is gained and lasts as long as NO flux is sustained. Upon removal of the latter, the process is reversible, and initial activities are recovered. By leaving the protein undamaged, ready to be reactivated as aconitase after [4Fe-4S] cluster re-insertion by a machinery left intact, NO in this context proves an authentic regulator rather than a somewhat toxic effector molecule. Like a hormone, NO has a short half-life. In addition, NOS dimerization, which is necessary to synthesize NO, can be interrupted under various pathophysiological conditions affecting availability of heme, tetrahydrobiopterin, or L-arginine (43). It is thus well established that in vivo, L-arginine is depleted in inflammatory settings or microbial infection as a consequence of arginase up-regulation (44-46). Cells must adjust to NO rise and fall, and some flexible transducer molecules have to cope with this obligate metabolic challenge. Our data support such a special role for IRP-1.

    FOOTNOTES

* This work was supported in part by Association pour la Recherche contre le Cancer Grant 5856.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 33-1-69-82-45-62; Fax: 33-1-69-07-72-47; E-mail: Jean-Claude.Drapier@icsn.cnrs-gif.fr.

Published, JBC Papers in Press, May 30, 2002, DOI 10.1074/jbc.M203276200

2 C. Bouton and J.-C. Drapier, unpublished data.

    ABBREVIATIONS

The abbreviations used are: IRP-1, iron regulatory protein, IRE; iron-responsive element, UTR, untranslated region; Tf-R, transferrin receptor; ISC, iron-sulfur cluster; NONOate, diazeniumdiolate; SPER/NO, spermine NONOate; DPTA/NO, dipropylenetriamine NONOate; DETA/NO, diethylenetetraamine NONOate; CHX, cycloheximide; CCCP, carbonyl cyanide m-chlorophenyl hydrazone; 2-ME, 2-mercaptoethanol; ABC, ATP binding cassette; DTT, dithiothreitol; IFN, interferon; LPS, lipopolysaccharide.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

1. Beinert, H., and Kiley, P. (1996) FEBS Lett. 382, 218-219[CrossRef][Medline] [Order article via Infotrieve]
2. Eisenstein, R. S. (2000) Annu. Rev. Nutr. 20, 627-662[CrossRef][Medline] [Order article via Infotrieve]
3. Drapier, J. C., Hirling, H., Wietzerbin, J., Kaldy, P., and Kühn, L. C. (1993) EMBO J. 12, 3643-3649[Medline] [Order article via Infotrieve]
4. Weiss, G., Goossen, W. D., Fuchs, D., Pantopoulos, K., Werner-Felmayer, G., Wachter, H., and Hentze, M. W. (1993) EMBO J. 12, 3651-3657[Medline] [Order article via Infotrieve]
5. Pantopoulos, K., and Hentze, M. W. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1267-1271[Abstract/Free Full Text]
6. Bouton, C., Oliveira, L., and Drapier, J. C. (1998) J. Biol. Chem. 273, 9403-9408[Abstract/Free Full Text]
7. Wardrop, S. L., Watts, R. N., and Richardson, D. R. (2000) Biochemistry 39, 2748-2758[CrossRef][Medline] [Order article via Infotrieve]
8. Bouton, C., Raveau, M., and Drapier, J. C. (1996) J. Biol. Chem. 271, 2300-2306[Abstract/Free Full Text]
9. Beinert, H., and Kennedy, M. C. (1993) FASEB J. 7, 1442-1449[Abstract]
10. Kennedy, M. C., Antholine, W. E., and Beinert, H. (1997) J. Biol. Chem. 272, 20340-20347[Abstract/Free Full Text]
11. Lill, R., and Kispal, G. (2000) Trends Biochem. Sci. 25, 352-356[CrossRef][Medline] [Order article via Infotrieve]
12. Strain, J., Lorenz, C. R., Bode, J., Garland, S., Smolen, G. A., Ta, D. T., Vickery, L. E., and Culotta, V. C. (1998) J. Biol. Chem. 273, 31138-31144[Abstract/Free Full Text]
13. Li, J., Kogan, M., Knight, S. A., Pain, D., and Dancis, A. (1999) J. Biol. Chem. 274, 33025-33034[Abstract/Free Full Text]
14. Land, T., and Rouault, T. A. (1998) Mol. Cell 2, 807-815[CrossRef][Medline] [Order article via Infotrieve]
15. Tong, W. H., and Rouault, T. (2000) EMBO J. 19, 5692-5700[CrossRef][Medline] [Order article via Infotrieve]
16. Drapier, J. C., and Hibbs, J. B., Jr. (1996) Methods Enzymol. 269, 26-36[Medline] [Order article via Infotrieve]
17. Leibold, E. A., and Munro, H. N. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 2171-2175[Abstract/Free Full Text]
18. Drapier, J. C., and Hibbs, J. B., Jr. (1988) J. Immunol. 140, 2829-2838[Abstract]
19. Oliveira, L., and Drapier, J. C. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 6550-6555[Abstract/Free Full Text]
20. Clementi, E., Brown, G. C., Feelisch, M., and Moncada, S. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 7631-7636[Abstract/Free Full Text]
21. Gardner, P. R., Costantino, G., Szabo, C., and Salzman, A. L. (1997) J. Biol. Chem. 272, 25071-25076[Abstract/Free Full Text]
22. Kispal, G., Csere, P., Prohl, C., and Lill, R. (1999) EMBO J. 18, 3981-3989[CrossRef][Medline] [Order article via Infotrieve]
23. Zheng, L., Kennedy, M. C., Blondin, G. A., Beinert, H., and Zalkin, H. (1992) Arch. Biochem. Biophys. 299, 356-360[CrossRef][Medline] [Order article via Infotrieve]
24. Gray, N. K., Pantopoulous, K., Dandekar, T., Ackrell, B. A., and Hentze, M. W. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 4925-4930[Abstract/Free Full Text]
25. Casey, J. L., Hentze, M. W., Koeller, D. M., Caughman, S. W., Rouault, T. A., Klausner, R. D., and Harford, J. B. (1988) Science. 240, 924-928[Abstract/Free Full Text]
26. Kennedy, M. C., Emptage, M. H., Dreyer, J. L., and Beinert, H. (1983) J. Biol. Chem. 258, 11098-11105[Abstract/Free Full Text]
27. Yang, W., Rogers, P. A., and Ding, H. (2002) J. Biol. Chem. 277, 12868-12873[Abstract/Free Full Text]
28. Nakai, Y., Yoshihara, Y., Hayashi, H., and Kagamiyama, H. (1998) FEBS Lett. 433, 143-148[CrossRef][Medline] [Order article via Infotrieve]
29. Leighton, J., and Schatz, G. (1995) EMBO J. 14, 188-195[Medline] [Order article via Infotrieve]
30. Savary, S., Allikmets, R., Denisot, F., Luciani, M. F., Mattei, M. G., Dean, M., and Chimini, G. (1997) Genomics 41, 275-278[CrossRef][Medline] [Order article via Infotrieve]
31. Csere, P., Lill, R., and Kispal, G. (1998) FEBS Lett. 441, 266-270[CrossRef][Medline] [Order article via Infotrieve]
32. Bekri, S., Kispal, G., Lange, H., Fitzsimons, E., Tolmie, J., Lill, R., and Bishop, D. F. (2000) Blood 96, 3256-3264[Abstract/Free Full Text]
33. Lutz, T., Westermann, B., Neupert, W., and Herrmann, J. M. (2001) J. Mol. Biol. 307, 815-825[CrossRef][Medline] [Order article via Infotrieve]
34. Auble, D. T., Hansen, K. E., Mueller, C. G., Lane, W. S., Thorner, J., and Hahn, S. (1994) Genes Dev. 8, 1920-1934[Abstract/Free Full Text]
35. Recalcati, S., Taramelli, D., Conte, D., and Cairo, G. (1998) Blood 91, 1059-1066[Abstract/Free Full Text]
36. Kim, S., and Ponka, P. (2000) J. Biol. Chem. 275, 6220-6226[Abstract/Free Full Text]
37. Nascimento, A., and Meneghini, R. (1995) Carcinogenesis 16, 1335-1338[Abstract/Free Full Text]
38. DeRusso, P. A., Philpott, C. C., Iwai, K., Mostowski, H. S., Klausner, R. D., and Rouault, T. A. (1995) J. Biol. Chem. 270, 15451-15454[Abstract/Free Full Text]
39. Kim, H. Y., LaVaute, T., Iwai, K, Klausner, R. D., and Rouault, T. A. (1996) J. Biol. Chem. 271, 24226-24230[Abstract/Free Full Text]
40. Sandau, K. B., Fandrey, J., and Brüne, B. (2001) Blood 97, 1009-1015[Abstract/Free Full Text]
41. Lok, C. N., and Ponka, P. (1999) J. Biol. Chem. 274, 24147-24152[Abstract/Free Full Text]
42. Seiser, C., Posch, M., Thompson, N., and Kühn, L. C. (1995) J. Biol. Chem. 270, 29400-29406[Abstract/Free Full Text]
43. Albakri, Q. A., and Stuehr, D. J. (1996) J. Biol. Chem. 271, 5414-5421[Abstract/Free Full Text]
44. Waddington, S. N. (2002) Kidney Int. 61, 876-881[CrossRef][Medline] [Order article via Infotrieve]
45. Gobert, A. P., Daulouede, S., Lepoivre, M., Boucher, J. L., Bouteille, B., Buguet, A., Cespuglio, R., Veyret, B., and Vincendeau, P. (2000) Infect. Immun. 68, 4653-4657[Abstract/Free Full Text]
46. Gobert, A. P., McGee, D. J., Akhtar, M., Mendz, G. L., Newton, J. C., Cheng, Y., Mobley, H. L., and Wilson, K. T. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 13844-13849[Abstract/Free Full Text]

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Fosset, M.-J. Chauveau, B. Guillon, F. Canal, J.-C. Drapier, and C. Bouton
RNA Silencing of Mitochondrial m-Nfs1 Reduces Fe-S Enzyme Activity Both in Mitochondria and Cytosol of Mammalian Cells
J. Biol. Chem., September 1, 2006; 281(35): 25398 - 25406.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Biederbick, O. Stehling, R. Rosser, B. Niggemeyer, Y. Nakai, H.-P. Elsasser, and R. Lill
Role of Human Mitochondrial Nfs1 in Cytosolic Iron-Sulfur Protein Biogenesis and Iron Regulation
Mol. Cell. Biol., August 1, 2006; 26(15): 5675 - 5687.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Gerber, K. Neumann, C. Prohl, U. Muhlenhoff, and R. Lill
The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins
Mol. Cell. Biol., June 1, 2004; 24(11): 4848 - 4857.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
C. Bouton and J.-C. Drapier
Iron Regulatory Proteins as NO Signal Transducers
Sci. Signal., May 13, 2003; 2003(182): pe17 - pe17.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Erlitzki, J. C. Long, and E. C. Theil
Multiple, Conserved Iron-responsive Elements in the 3'-Untranslated Region of Transferrin Receptor mRNA Enhance Binding of Iron Regulatory Protein 2
J. Biol. Chem., November 1, 2002; 277(45): 42579 - 42587.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available