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J. Biol. Chem., Vol. 277, Issue 40, 37464-37468, October 4, 2002

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A Novel Ligand-binding Domain Involved in Regulation of Amino Acid Metabolism in Prokaryotes^*

Thijs J. G. Ettema^§, Arie B. Brinkman, Travis H. Tani^¶, John B. Rafferty, and John van der Oost

From the  Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, NL-6307 CT Wageningen, The Netherlands, the  Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom, and the ^¶ Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-1055

Received for publication, June 18, 2002, and in revised form, July 15, 2002

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

A combination of sequence profile searching and structural protein analysis has revealed a novel type of small molecule binding domain that is involved in the allosteric regulation of prokaryotic amino acid metabolism. This domain, designated RAM, has been found to be fused to the DNA-binding domain of Lrp-like transcription regulators and to the catalytic domain of some metabolic enzymes, and has been found as a stand-alone module. Structural analysis of the RAM domain of Lrp reveals a -fold that is strikingly similar to that of the recently described ACT domain, a ubiquitous allosteric regulatory domain of many metabolic enzymes. However, structural alignment and re-evaluation of previous mutagenesis data suggest that the effector-binding sites of both modules are significantly different. By assuming that the RAM and ACT domains originated from a common ancestor, these observations suggest that their ligand-binding sites have evolved independently. Both domains appear to play analogous roles in controlling key steps in amino acid metabolism at the level of gene expression as well as enzyme activity.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Allosteric regulation is a general mechanism that enables a tight control of both enzyme activity and gene expression. A textbook example of this mechanism is the feedback inhibition of enzymes that catalyze key steps in amino acid biosynthesis. Important insight in the molecular basis of modulated enzyme activity has been provided by the crystal structure of 3-phosphoglycerate dehydrogenase (SerA) (1, 2), an enzyme that catalyzes the rate-limiting first step in serine biosynthesis. The SerA structure has revealed separated catalytic and regulatory domains that are connected by a flexible hinge. The structural motif of the regulatory domain consists of a four-stranded anti-parallel -sheet with two -helices packed on one side. When the serine effector molecule binds to this -sandwich, the hinge region allows allosteric regulation: a slight interdomain rearrangement that down-regulates the catalytic activity of the enzyme (2, 3).

A thorough sequence profile analysis has shown that the SerA regulatory domain is an ancient small molecule binding domain (SMBD)¹ that is conserved in a wide variety of enzymes as well as in some transcriptional regulators that are involved in the control of amino acid and purine metabolism (4). As predicted in the latter study, the presence of this ancient "ACT domain" (for review see Ref. 5) was indeed demonstrated in the recent crystal structures of rat phenylalanine hydroxylase (6), an enzyme that catalyzes the conversion of phenylalanine to tyrosine. Interestingly, the structure of the Escherichia coli threonine deaminase (7), the enzyme catalyzing the first step of the isoleucine biosynthesis pathway, revealed two domains that resembled the ACT domain of SerA at the structural level rather than at the sequence level (4). This observation demonstrates that the sequence divergence of SMBDs like ACT can expand beyond the detection limits of the sequence-based algorithm of PSI-BLAST (8). For this reason, the threonine deaminase-ACT domains have been referred to as "ACT-like" domains (5).

In the present study we describe a novel ligand-binding module that we named the RAM domain because of its general involvement in the allosteric Regulation of Amino acid Metabolism. This domain is mainly found in association with a class of prokaryotic transcriptional regulators but also as a module in enzymes and in some instances as stand-alone SMBD.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

PSI-BLAST Analysis and Multiple Sequence Alignments, Domain Analysis of Proteins-- In order to verify and characterize the relationship between distant RAM domains at the sequence level, we performed several PSI-BLAST searches (8) at the National Center of Biotechnology Information. When a PSI-BLAST search was seeded with the C-terminal domain of the Pyrococcus furiosus LrpA (residues 62-141), using a BLOSUM80 matrix and an expect value threshold of 0.001, the first stand-alone versions of RAM (lacking the HTH domain) were retrieved within the first iteration (10580664; E = 6 × 10¹²); the RAM domains within the Sulfolobus solfataricus and Sulfolobus tokodaii 2-isopropylmalate synthase (13814162, respectively, 15623321) were recovered in iteration 3 (E = 7 × 10⁶). A reverse PSI-BLAST using the S. solfataricus 2-isopropylmalate synthase sequence (residues 342-461) recovered HTH-RAM proteins (e.g. 13813287 at iteration 1, E = 3 × 10⁵) and stand-alone versions of the RAM domain (e.g. 13813398 at iteration 2, E = 5 × 10⁵), thereby connecting the most distant RAM domain containing proteins with each other on a statistical basis. A multiple alignment of RAM domains was constructed by collecting the "highest scoring pairs of sequence segments" using PSI-BLAST, which were re-aligned using ClustalW (9), followed by minor manual adjustment based on the secondary structure. Domain analysis of RAM- and ACT-containing proteins was performed using SMART (10) an PFAM (11). All protein sequences were extracted from ENTREZ.

Structural Alignment and Superimposition Three-dimensional Structures of SerA ACT Domain and LrpA RAM Domain-- In order to detect the structural similarity of ACT and RAM, a superimposition of the C-terminal domain of the P. furiosus LrpA (residues 64-135) and the ACT domain present in the SerA of E. coli (residues 335-410, PDB entry 1PSD) was constructed using the Swiss PDB viewer (12) using the "Iterative fit" option. The superimposition was constructed with 49 -carbon atoms that displayed a root mean square value of less than 2.5 Å, comprising about 65% of the domains. From the superimposed structures, a structural alignment was deduced using the Structural alignment tool of the Swiss PDB viewer (12).

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

The Structure of the C-terminal Domain of LrpA Resembles Structure of the ACT Domain-- The Lrp family of transcriptional regulators plays a crucial role in the control of amino acid metabolism in prokaryotes. Although the leucine-responsive regulatory protein (Lrp) from E. coli is a global transcriptional regulator (13), most Lrp homologs act as specific regulators (e.g. Refs. 14-16). The interaction of specific amino acid effectors with Lrp-like regulators may lead to modulation of (i) DNA affinity, (ii) DNA bending, (iii) Lrp oligomeric state (dimer/tetramer/octamer/hexadecamer), and (iv) Lrp tertiary structure (13, 15, 16, 17). All these changes most likely reflect an allosteric regulation of Lrp activity by a (minor) structural rearrangement.

Recently, the structure of an archaeal Lrp homolog, the P. furiosus LrpA octamer (or tetramer of dimers), has been resolved (18). The structure of the LrpA monomer revealed an N-terminal DNA-binding helix-turn-helix (HTH) domain, an extended hinge, and a C-terminal globular domain with a -fold. Previous mutagenesis analyses were in perfect agreement with the N-terminal domain being involved in the interaction with DNA and predicted the C-terminal -sandwich to have a regulatory function (18, 19). What was not noted during the initial analysis of the LrpA structure is the interesting fact that the C-terminal regulatory domain of LrpA appears to resemble the ACT domains (4, 5) with respect to structure and function; both consist of a typical -sandwich and are anticipated to be regulatory domains involved in allosteric modulation of the activity of enzymes and DNA-binding proteins that are involved in amino acid metabolism. Despite the similarity in structure with the ACT domain, we propose that, for reasons discussed below, the C-terminal regulatory domain of LrpA is part of a novel, distinct class of regulatory domains.

The overall structural resemblance between the RAM domain of the P. furiosus LrpA and the ACT domain of the E. coli SerA is confirmed by superimposition of both structures (Fig. 1a). The obtained root mean square deviation value between the LrpA-RAM and the SerA-ACT (1.8 Å) compares with a value obtained with a superimposition between SerA-ACT and the ACT domain of the rat phenylalanine hydroxylase (1.7 Å).

View larger version (41K): [in this window] [in a new window]   Fig. 1.   a, superimposition of the RAM domain of the P. furiosus LrpA (purple) with the ACT domain of the E. coli SerA (yellow) showing the strong similarity between the two domains at the structural level (root mean square deviation value 1.8 Å). The superimposition was constructed with 49 -carbon atoms, which composes about 65% of the domains. In addition, the position of the negative effector of SerA ACT domain, serine, was indicated within the superimposed domains. Superimposition and figure were created using the Swiss PDB viewer (19). b, structural alignment of the RAM domain of the P. furiosus LrpA (residues 64-135) and the ACT domain of the E. coli SerA (residues 335-410). In addition, the 80% consensus sequences for RAM and ACT domains are included in the alignment indicating the sequence divergence between the two SMBDs. For abbreviations of the different amino acid classes see Fig. 2. Matched residues that display a root mean square value of less than 2.5 Å are boxed. The structural alignment was constructed using the Swiss PDB viewer (19) using the "Structural alignment" option. c, structural comparison of the RAM dimer of P. furiosus LrpA (left) and ACT dimer of E. coli SerA (right). The monomers are shown in cyan and blue, and the ligand response mutations of the RAM domain and the ACT domain corresponding to those that are depicted in Fig. 2, a and b, respectively, were mapped into the backbones of the respective structures in red with magenta side chains.

Ligand Response Mutations in RAM and ACT Suggest a Different Location of the Ligand-binding Sites in RAM and ACT-- Despite the structural similarities between the RAM domain and the ACT domain, however, the effector-binding sites in these domains seem to be different. In the ACT domain of SerA, the loop that links the first -strand (1) and the first -helix (1) of the -fold, makes up the binding pocket of the serine effector. The important role of this loop is in agreement with the fact that it is very well conserved in ACT-containing enzymes and regulators (Fig. 2a). An invariant glycine residue, which is also conserved in the ACT-like domains of the E. coli threonine deaminase, and an adjacent hydrophobic residue have been proposed to be involved in maintenance of the strand-helix interface; two additional conserved polar residues are involved in binding the ligand with hydrogen bonds (1, 4). The importance of the conserved region was also confirmed by mutation analysis of other ACT-containing enzymes from E. coli, i.e. the valine-binding regulatory subunit of the acetolactate synthase (IlvH) (20) and the lysine-sensitive aspartokinase (LysC) (21) (Fig. 2a). Mutations that resulted in a strongly reduced or abolished response after being exposed to their respective effector (ligand response mutations) all cluster within the conserved loop region, suggesting that binding of the effector resembles the interaction of SerA with serine. Mapping the ACT ligand response mutations into the structure of the ACT dimer of SerA (Fig. 1c) confirms this idea. The ligand response mutations cluster at the dimer interface in general and at the ligand (serine)-binding site in particular (Fig. 1c).

View larger version (86K): [in this window] [in a new window]   Fig. 2.   a, an alignment of the most diverse members of the ACT domain. The secondary structure assignment that is indicated above the alignment was derived from the crystal structure of the E. coli 3-phosphoglycerate dehydrogenase (SerA; PDB code 1PSD). The amino acid residues that are involved in the binding of the effector serine in SerA, are indicated above the alignment (x). In addition, the ligand response mutations that were determined for the E. coli small subunit of the acetolactate synthase (IlvH) and aspartokinase (LysC) are indicated with * and $, respectively. The 80% consensus shown below the alignments was obtained as described above, and the position numbers on the left side indicate the limits of the domains. Also a structural alignment of the two regulatory ACT-like domains of the E. coli threonine deaminase (THD1) is included, with a mapped ligand response mutation (+), indicating the divergence on the sequence level between these domains and the genuine ACT domains. Secondary structure from the first repeat of THD1 is indicated above the alignment. The 80% consensus shown below the alignments was obtained using the following amino acid classes (4): small (s, ACGSTDNVP; shaded yellow), polar (p, YWHKREQDNST; shaded green), big (b, FILMVWYKREQ; gray shading), and hydrophobic (h, ILVCAGMFYWHTP; shaded black). Of the hydrophobic residues, the aliphatic subset (h, ILVA) is in black with red shading. The position numbers on the left side indicate the limits of the domains. b, a multiple alignment of RAM domains. By using the structure of P. furiosus LrpA (7), the secondary structure of the RAM domain has been deduced, as is indicated above the alignment (E, -strand; H, -helix). The sequences are grouped by domain architecture as follows: 1, Lrp-like transcriptional regulators and homologs (HTH-RAM); 2, stand-alone versions of the RAM domain; 3, duplication of Lrp-like transcriptional regulator; and 4, RAM domain present in crenarchaeal 2-isopropylmalate synthase. The ligand response mutations as determined for the E. coli Lrp by Platko and Calvo (19) are indicated above the alignment (#). All protein sequences were obtained from ENTREZ, and GenBankTM identifiers are indicated for each protein. Species abbreviations are as follow: Aea, Aquifex aeoliticus; Afu, Archaeoglobus fulgidus; Atu, Agrobacterium tumefaciens; Bsu, Bacillus subtilis; Ccr, Caulobacter crescentus; Eco, E. coli; Mlo, Mesorhizobium loti; Mtu, Mycobacterium tuberculosis; Pab, Pyrococcus abyssi; Pfu, P. furiosus; Pho, Pyrococcus horikoshii; Pmi, Proteus mirabilis; Pmu, Pasteurella multocida; Ppu, Pseudomonas putida; Sco, Streptomyces coelicolor; Sno, Streptomyces noursei; Sso, S. solfataricus; Sto, S. tokodaii; and Tvu, Thermoplasma vulcanium.

The dimer structure of the SerA-ACT differs significantly from the LrpA-RAM dimer. Whereas the contact between the two ACT domains seems to be mediated via the 2 and 3 interface resulting in an eight-stranded anti-parallel -sheet (1), in LrpA the RAM dimer is mainly formed by interactions between the antiparallel -sheets that are facing each other, forming an antiparallel -barrel-like structure (Fig. 1c) (18). Because of this structural difference, and given the fact that the ligand-binding site for ACT domains is located at the dimer interface, this might imply that the ligand-binding site is different in RAM domains. Indeed, the region that is involved in interaction with the ligand (Fig. 2a) is well conserved in ACT domains, whereas the corresponding loop that links strand 1 and helix 1 in RAM domains displays only poor sequence conservation (Fig. 2b). In RAM, the best conserved region, again including an invariant glycine residue, appears to be the region surrounding the loop connecting strands 2 and 3. Moreover, extensive mutagenesis studies that have been performed with the E. coli Lrp (19) confirm the importance of this region with respect to ligand response. The Lrp leucine response mutations that were obtained in this study apparently lost the capacity to bind their ligand (19). When the equivalents of these E. coli Lrp ligand response mutations are mapped into the structure of the LrpA-RAM dimer of P. furiosus, it becomes clear that five of seven mapped mutations belonging to this class (Leu-95, Met-101, Ala-134, Ile-135, and Ile-136) are clustered in a region across the dimer interface (Fig. 1c). The remaining two ligand response mutations (Gly-111 and Gly-123) are located in close proximity to the other mutations, albeit in adjacent dimers of the octamer rather than within the same dimer (18) (not shown in Fig. 1c). These observations suggest that the ligand-binding site of RAM is located at a different position than that of ACT. Based on the relatively high sequence conservation and to some extent on the mutation data, it is tempting to speculate that the ligand-binding site of RAM is located between the 2 and 3. Lrp ligand co-crystallization experiments are required to confirm this hypothesis.

The RAM Domain Has a Wide Phyletic Distribution and Is Present in Transcriptional Regulators, Metabolic Enzymes, and as a Stand-alone Version-- In order to verify and characterize the relationship between distant RAM domains at the sequence level, we performed several iterative data base searches (PSI-BLAST) (8) at the National Center of Biotechnology Information, revealing the ubiquitous phyletic distribution of this domain among Archaea and Bacteria (>250 proteins after 5 iterations). The proteins that were retrieved during this search were dominated by the Lrp-like regulators, typically consisting of an N-terminal DNA-binding helix-turn-helix domain fused to a C-terminal RAM domain (HTH-RAM). An interesting variant of HTH-RAM is a duplicated version, found in some bacteria (Streptomyces sp.); this supports the view that the native form of Lrp-like transcriptional regulators is at least a dimer configuration. In addition, examples were found of a RAM domain that was fused to the C terminus of 2-isopropylmalate synthase (IPMS) in the Crenarchaea S. solfataricus (SSO0977), S. tokodaii (ST1301), and Pyrobaculum aerophilum (PAE1986). Also several stand-alone RAM domains were detected with the PSI-BLAST search (Fig. 3). This variable domain architecture of RAM again resembles that of the ACT domain. ACT is found as an allosteric regulatory domain associated with metabolic enzymes (like SerA, phenylalanine hydroxylase and threonine deaminase) and transcriptional regulators (like TyrR and PhhR) as well as a stand-alone version (IlvH) (Fig. 3). It is noted that the RAM domains are mainly associated with transcriptional regulators, whereas the ACT domain is most often found as a regulatory module of metabolic enzymes.

View larger version (33K): [in this window] [in a new window]   Fig. 3.   Domain architectures of ACT and RAM containing proteins, subdivided into three classes according to their function. For each class of proteins, the phyletic distribution is depicted below the domain structure. Domain analysis was performed using SMART (20). Apart from the regulatory domains (ACT or RAM), the other domains that are part of the proteins are described as follows. a, transcription regulation-associated regulatory domains. 1, Lrp-like transcriptional regulators, generally consisting of an N-terminal DNA-binding helix-turn-helix domain fused to a C-terminal RAM domain; 2, duplicated form of Lrp-like transcriptional regulator, consisting of a tandem repeat of the transcriptional regulator; 3, transcriptional regulator of aromatic amino acid biosynthesis, containing an N-terminal ACT domain, followed by a PAS domain which is possibly involved in signal sensing. The C-terminal part of these regulators contain a sigma54 interacting domain (PF00989) and a DNA-binding helix-turn-helix (hth_8; PF02954). b, enzyme-associated regulatory domains. 1, Crenarchaeal 2-isopropylmalate synthases, containing an HMGL-like domain, which is found in a diverse set of enzymes including several aldolases and a pyruvate carboxylase; 2, aspartokinases, containing an N-terminal kinase domain (PF00696) that is involved in phosphorylation of a variety of amino acid substrates; 3, diverse group of proteins involved in biosynthesis of aromatic amino acids consisting of prephenate hydratases, chorismate mutases, and phenylalanine hydroxylases. From the latter enzyme the domain architecture is displayed, containing a biopterin_H domain (PF00351), which is present in biopterin-dependent aromatic amino acid hydroxylases; 4, group of proteins consisting of 3-phosphoglycerate, homoserine, and malate dehydrogenases, containing an N-terminal 2-Hacid_DH catalytic domain (PF00389) and a NAD-binding domain (2-Hacid_DH_C, PF02826); 5, formyltetrahydrofolate deformylases, typically containing a C-terminal formyl_transf. domain (PF00551), a domain that is present in multiple enzymes that are involved in de novo purine biosynthesis; 6, diverse set of proteins containing uridylyltransferases that are involved in glutamine synthase regulation (GlnD), guanosine polyphosphate 3'-pyrophosphorylases (SpoT), and GTP pyrophosphokinases (RelA). The latter two enzymes are involved in stringent response. The domain architecture that is depicted here represents GlnD, containing a nucleotidyltransferase domain (NTP_transf, PF01909) and an HDc domain that is involved in metal-dependent phosphohydrolase activity. c, stand-alone regulatory domains. 1, small regulatory domain of the acetolactate synthase, generally consisting of a N-terminal ACT domain fused to a small domain that is probably involved in the interaction with the large subunit (IlvI); 2, isolated RAM domains. The function of these proteins is still to be elucidated; however, it is possible that they play a role analogous to the isolated ACT domains, i.e. regulatory subunit of enzymes or transcriptional regulators.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

The -motif appears to be a common regulatory structure in amino acid metabolic enzymes and transcriptional regulators; both the RAM and the ACT domains share this fold and are associated with proteins that are involved with amino acid metabolism either as part of enzymes, as part of transcriptional regulators, or as stand-alone SMBD. Apart from the structural and functional similarity between the two domains, another connection is the fact that the expression of some bacterial ACT-containing enzymes (e.g. SerA and IlvHI) is under control of RAM-containing transcriptional regulators of the Lrp family (9). These observed analogies between RAM and ACT may be useful for speculating about the function of uncharacterized RAM and ACT domains. For example, the function of the stand-alone versions of the RAM domains that are present in several bacterial and archaeal genomes is yet unclear. However, the function of stand-alone ACT domains might suggest the possible function of their stand-alone RAM counterparts. For example, the acetolactate synthase in E. coli is a key enzyme in branched chain amino acid biosynthesis that is subjected to valine feedback inhibition (19). The heterotetrameric holoenzyme is made up of the large catalytic subunit (IlvI) and a small regulatory subunit that consists of a single ACT domain (IlvH) and accounts for the valine-mediated feedback repression. It is possible that a stand-alone RAM domain performs a function that is analogous to that of the IlvH subunit, allosteric regulation of enzymes (or possibly transcriptional regulators) involved in amino acid metabolism via protein-protein interactions. Interestingly, profile-based analysis of prokaryal genomes (e.g. S. solfataricus) failed to identify a gene encoding the small regulatory subunit of the acetolactate synthase (IlvH), whereas homologs of the gene encoding the large catalytic subunit of the acetolactate synthase could be identified on the genome (e.g. ilvB-1). Possibly, the large subunit of the acetolactate synthase present in these organisms are subjected to allosteric regulation by the stand-alone RAM domains that are encoded on the genome.

A more clear-cut example is the anticipated role for the RAM domain that is present at the C terminus of the crenarchaeal IPMS. Generally, IPMS catalyzes the first step in leucine biosynthesis that is subjected to leucine-mediated feedback inhibition. Leucine has the following two known effects on this enzyme in Salmonella typhimurium: (i) it controls the catalytic activity of the enzyme by feedback inhibition (22), and (ii) it causes a dissociation of the tetrameric enzyme into its monomeric subunits (23). Genetic mapping of leucine-insensitive mutants (24, 25) revealed that the mutation resulting in the affected end product inhibition is located at the C terminus of the S. typhimurium IPMS. So the domain that is probably responsible for the allosteric control of IPMS is located in the C-terminal part of the enzyme. Sequence analysis of the C-terminal part of the S. typhimurium IPMS did not reveal the presence of a RAM or ACT domain, possibly suggesting the presence of yet another alternative regulatory domain. Although the exact mechanism of inhibition remains to be elucidated, the presence of a C-terminal RAM domain in the crenarchaeal RAM-IPMS strongly suggests that these enzymes are subjected to RAM-mediated feedback regulation.

The date presented here indicate that structure-based alignments of the regulatory domains as well as extensive (reverse) PSI-BLAST analyses fail to close the apparent gap in sequence divergence between RAM and ACT. This might suggest that ACT and RAM both have independently evolved into allosteric regulatory domains. However, the most parsimonious scenario is that the two domains have emerged from a common ancestor and only evolved different interaction specificity with respect to their ligands. Possibly, the diversification in binding sites is the underlying reason of the dramatic sequence divergence between RAM and ACT, with the overall structure being well conserved. This appears to be yet another example of domain evolution in which sequence similarity has been lost while retaining their structural similarity (26).

	ACKNOWLEDGEMENT

We thank Dr. Eugene V. Koonin (National Center of Biotechnology Information, Bethesda) for stimulating discussions.

	FOOTNOTES

^* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The atomic coordinates and the structure factors (code 1I1G) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

^§ To whom correspondence should be addressed. Tel.: 31-317-483110; Fax: 31-317-483829; E-mail: Thijs.Ettema@algemeen.micr.wau.nl.

Published, JBC Papers in Press, July 23, 2002, DOI 10.1074/jbc.M206063200

	ABBREVIATIONS

The abbreviations used are: SMBD, small molecule binding domain; PDB, Protein Data Bank; IPMS, 2-isopropylmalate synthase; HTH, helix-turn-helix.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

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