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FIG. 1.
Schematic maps and the phenotypes summarized of the mutants analyzed in
this study. Vectors are named as in
Table I, as indicated with
Footnote a. Footnote b shows the conserved region among DP1s that shows
homology to the eukaryote small subunit of DNA polymerase 
is indicated
in the box colored in yellow. The mini-intein insertion site and the
catalytic center for polymerization in DP2 are shown with arrows and
vertical lines. The region that shows homology to the catalytic
subunit of yeast DNA polymerase 
in DP2 is indicated in the box
colored in red. Two cysteine clusters are indicated with boxes
filled with horizontal lines. The numbers in
bold and blue in the parentheses are the numbers of
peptide without the intein. Footnote c indicates the thermostability of the
truncated peptide (ther. stab.), defined as the presence (+) or
absence (-) of specific bands on SDS-GAGE analysis of the supernatant after
heating at 85 °C for 30 min (Fig.
3). Subunit Inter., subunit interaction, the ability of
complex formation between DP1Pho and DP2Pho after purification steps;
Pol., polymerization; Exo., 3'-5' exonuclease
activities; +, positive; -, negative; blank, not checked.
