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J. Biol. Chem., Vol. 278, Issue 44, 42785-42794, October 31, 2003
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From the
IRBB-Barcelona Science Park, Josep Samitier, 1-5, E-08028 Barcelona, Spain, **Biomedical Research Institute "Alberto Sols," Arturo Duperier, 4, E-28029 Madrid, Spain, ¶Department of Biochemistry and Molecular Biology, University of Barcelona, Martí Franquès, 1, E-08028 Barcelona, Spain, INSERM U402, Faculte de Médecine Saint-Antoine, 27, rue Chaligny, F-75571 Paris Cedex 12, France, Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti, 435, I-20141 Milan, Italy, and the ¶¶Endocrinology and Diabetes Unit, Hospital Clínic de Barcelona and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Villarroel, 170, E-08036 Barcelona, Spain
Received for publication, July 30, 2003
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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. Experiments with a specific inhibitor of ERK1/2 activation and kinase assays indicate that these proteins were directly involved in the stimulation of glycogen synthase and glycogen synthesis induced by tungstate without a direct involvement of protein kinase B (PKB/Akt). These results show a direct involvement of ERK1/2 in the mechanism of action of tungstate at the hepatic level. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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-cell population in neonatally streptozotocin-treated rats (2). In vitro, tungstate or pertungstate treatment mimics most of the metabolic effects of insulin in primary cultured adipocytes, which include the stimulation of lipogenesis, hexose uptake, glucose oxidation, and inhibition of lipolysis (6). In contrast to other transition metal derivatives, sodium tungstate has a low toxicity profile in both short and long term treatments (for a review, see Refs. 9 and 10). Despite considerable data on the pharmacological and metabolic effects of sodium tungstate, there is a lack of information on its molecular mechanisms of action. This compound has been described as a phosphatase inhibitor (11–16). Interestingly, Foster et al. (14) described the inhibitory effects of this compound on glucose-6-phosphatase in vitro, an action that may contribute to a decrease in gluconeogenic activity in vivo.
As described above, tungstate mimics most of the metabolic effects of insulin. The initial steps in insulin action involve the binding of this hormone to its receptor and the subsequent activation of its tyrosine kinase activity. This process results in the phosphorylation of several substrates, which, among other actions, ultimately trigger the activation of glycogen synthesis. Stimulation of glycogen deposition is one of the most significant consequences of insulin action in liver. Therefore, the analysis of the effects of tungstate on glycogen deposition may greatly contribute to identify its mechanism of action.
Here we present the first analysis of tungstate action on several components of the insulin transduction cascade. Tungstate exerted insulin-like actions in primary cultured rat hepatocytes as it increased glycogen deposition. The actions of this compound were not mediated by the insulin receptor (IR)1 as its phosphorylation state remained unchanged after treatment. In contrast, there was a clear transient phosphorylation and consequent activation of ERK1/2. These protein kinases triggered the phosphorylation of glycogen synthase kinase-3 (GSK3) and p90rsk1, and ultimately, the activation of glycogen synthase (GS). These data indicate that ERK1/2 plays a key role in tungstate-stimulated glycogen synthesis in liver and therefore contributes to the antidiabetic action of this compound.
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EXPERIMENTAL PROCEDURES |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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/ and c-Myc antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-p44/42 MAPK (Thr-202/Tyr-204), phospho-GSK-3 (Ser-9), phospho-p90rsk (Ser-380), PKB/Akt, phospho-PKB/Akt (Thr-308), and phospho-PKB/Akt (Ser-473) antibodies were from Cell Signaling (Beverly, MA). Horseradish peroxidase-conjugated secondary antibodies were from Cell Signaling. PD98059 was from Sigma. Enzymes and biochemical reagents were from Sigma, unless otherwise indicated. All other chemicals were of analytical grade. Cell Lines—CHO-R cells (17) were cultured in Ham's-F12 nutrient mixture supplemented with 10% FCS and 0.7 mg/ml Geneticin. 293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FCS.
Hepatocyte Isolation and Culture—Collagenase perfusion was used to isolate hepatocytes from male Wistar rats (180–225 g) fasted for 24 h, as described previously (18). Cells were suspended in DMEM, supplemented with 10 mM glucose, 10% FCS, 100 nM insulin, and 100 nM dexamethasone (Sigma) and then seeded onto plastic plates of 60-mm diameter treated with 0.1% gelatin (Sigma) at a final density of 8 x 104 cells/cm2. After cell attachment (4 h at 37 °C), the medium was replaced by DMEM without glucose, FCS, and hormones, or alternatively, by DMEM with 5 mM glucose, 10% FCS. Hepatocytes were incubated a further 14–20 h at 37 °C before starting the experiments.
Glycogen and GS Measurement—To measure glycogen content, cell monolayers were scraped into 30% KOH, and the extract was then boiled for 15 min and centrifuged at 5000 x g for 15 min. Glycogen was measured in the cleared supernatants, as described (19).
Glycogen synthesis was measured after incubation of primary cultured hepatocytes with 2 µCi/ml [U-14C]glucose (PerkinElmer Life Sciences) for 2 h in DMEM with 10 mM glucose. On termination of the incubations, hepatocyte monolayers were washed three times with 150 mM NaCl and extracted in 0.1 M NaOH. Extracts were deproteinized with trichloroacetic acid (10%, w/v) containing glycogen carrier, and the radioactivity incorporated into glycogen was measured.
To measure GS activity, frozen cell monolayers were scraped using 100 µl of homogenization buffer, which consisted of 10 mM Tris-HCl (pH 7.0), 150 mM KF, 15 mM EDTA, 15 mM 2-mercaptoethanol, 10 µg/ml leupeptin, 1 mM benzamidine, and 1 mM phenylmethylsulfonyl fluoride. Homogenization was performed at 4 °C with a Polytron homogenizer. Homogenates were centrifuged at 10,000 x g for 15 min at 4 °C; supernatants and sediments were recovered for determinations. Sediments were resuspended in 100 µl of the same buffer. GS activity was measured in the presence or absence of 6.6 mM glucose-6-phosphate (20). The ratio of these two activities (–glucose-6-phosphate/+glucose-6-phosphate) is an estimate of the activation state of the enzyme.
Cell Treatments and Phosphorylation Analysis—CHO-R cells were grown to confluence and then deprived of FCS for 14–16 h. Tungstate and insulin treatments were performed in serum-free medium unless otherwise indicated. Plates were flash-frozen in liquid nitrogen and processed for protein extract preparation. Cells were scraped with 400 µl/60-mm plate of cold 30 mM Tris-HCl, pH 7.4, 25 mM NaCl, 1% (v/v) Triton X-100, 0.1% SDS, 10 mM sodium fluoride, 10 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1 mM EGTA, 20 nM okadaic acid, 10 µg/ml aprotinin, 10 µg/ml leupeptin, 10 µg/ml pepstatin. After 10 min on ice, extracts were centrifuged 10 min at 4 °C at 13,000 x g. Protein concentration from the supernatants was measured using the Bio-Rad protein assay. Proteins were separated by SDS-PAGE, transferred to nitrocellulose membranes (Schleicher & Schüll), and immunoblotted with selected antibodies; the immunoblots were developed using an enhanced chemiluminescence detection system (Amersham Biosciences), following the manufacturer's instructions. ERK1/2 activity was measured after immunoprecipitation of cell extracts with the phospho-p44/42 MAPK antibody and assayed with recombinant ELK1 as substrate using the p44/42 MAPK assay kit (Cell Signaling).
Transfection Experiments—These were performed as described in Ref. 21. Briefly, 293T cells were plated 24 h before transfection at a density of 3 x 105 cells/60-mm plate and transfected with empty plasmid pSG5 or the derived vector containing Pyst1 cDNA. Cells were incubated in medium containing 0.5% FCS for 24 h and treated with 1 mM tungstate for 1 h. They were then treated with 100 nM epidermal growth factor for 5 and 10 min in the presence or absence of 1 mM tungstate, and ERK1/2 phosphorylation was analyzed by Western blot as described above.
Statistical Analysis—Results were analyzed for significance by analysis of variance and unpaired Student's t test. p < 0.05 was considered significant.
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RESULTS |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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Tungstate Treatment Does Not Phosphorylate the Insulin Receptor in CHO-R Cells or Primary Cultured Hepatocytes— Our first approach was to compare the effects of insulin and tungstate on the tyrosine phosphorylation profile in Chinese hamster ovary cells that overexpress human wild type insulin receptor (CHO-R) (17). This is a suitable cellular model to study the downstream events of the insulin transduction cascade. CHO-R cells were treated with insulin or with increasing concentrations of sodium tungstate for 5, 10, 60 min, and overnight (14 h). Cell extracts were then analyzed by Western blot using antibodies that react to phospho-tyrosine residues. Insulin produced a rapid phosphorylation of the -subunit of its receptor, and several higher molecular weight bands, one of which had a molecular weight compatible with the insulin receptor substrate-1 (IRS-1) (Fig. 2, A–C). In contrast, in cells treated with tungstate, there was no significant phosphorylation of the IR -subunit at the concentrations assayed neither in short (Fig. 2B) nor in long term (Fig. 2C) treatments. Co-incubation with tungstate plus insulin did not increase the phosphorylation of the IR -subunit above the levels observed when the cells were treated with insulin alone (not shown).
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Similarly, when primary cultured hepatocytes were incubated with tungstate under conditions in which the compound stimulated glycogen deposition, no significant increase in the phosphorylation of the IR -subunit was observed (Fig. 2D). In contrast, incubation with insulin, which also stimulated glycogen deposition to a similar extent, induced the phosphorylation of this receptor (Fig. 2D).
Since tungstate is a phosphatase inhibitor, we tested whether this compound could prevent IR dephosphorylation in CHO-R cells stimulated by insulin and thus improve downstream insulin signaling. Briefly, CHO-R cells were treated with insulin for 10 min, the hormone was removed, and the effect of tungstate on the phosphorylation state of the IR -subunit was evaluated by Western blot analysis at a range of time points. No significant differences were detected between tungstate-treated and untreated cells (Fig. 2E). These results indicate that tungstate increased glycogen deposition without modifying the IR -subunit phosphorylation.
Tungstate Treatment Activates ERK1/2 in CHO-R Cells and Primary Cultured Hepatocytes—The tyrosine phosphorylation pattern in tungstate-treated CHO-R cells showed an additional protein band of 40–44 kDa, which was also present in insulin-treated cells (Fig. 2F). The molecular mass of the phosphorylated band was compatible with MAPK family members.
To check whether this protein corresponded to a MAPK, we performed a series of Western blots in extracts from CHO-R cells and primary cultured hepatocytes using antibodies that recognize ERK1/2 only when phosphorylated at conserved threonine 202 and tyrosine 204 (numbering corresponding to human MAPK (Erk1)). Transient phosphorylation of ERK1 and 2 was observed when CHO-R cells were treated either with insulin or with tungstate; it peaked at 5–10 min, decreasing thereafter (Fig. 3A). Furthermore, analysis with antibodies against total ERK1/2 showed that these treatments did not significantly modify the total levels of these two kinases (Fig. 3A). We also observed an increase in the phosphorylation of ERK1/2 in primary cultured hepatocytes incubated with 1 mM tungstate, which peaked at about 10 min, decreasing thereafter (Fig. 3B). There was no significant increase in the total amount of ERK1/2 (Fig. 3B). No synergistic effect was observed when CHO-R cells or primary cultured hepatocytes were simultaneously incubated with tungstate and insulin (not shown).
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ERK1/2 phosphorylation at consensus residues triggers the activation of these kinases (22, 23). To confirm that these phosphorylated kinases were catalytically active, we assayed ERK1/2 activity in immunoprecipitates from CHO-R cells treated either with tungstate or with insulin. Recombinant transcription factor ELK-1 was used as substrate, and the phosphorylation was monitored by Western blot with antibodies that recognize this protein when phosphorylated by ERK1/2 at serine 383. Tungstate and insulin treatment increased ERK1/2 activity (Fig. 3C), a finding consistent with the phosphorylation data shown in Fig. 3, A and B.
As a phosphatase inhibitor, the effect of tungstate on ERK1/2 phosphorylation may be related to the inhibition of a MAPK-phosphatase. Pyst1 (MKP-3, rVH6) is the main MAPK phosphatase involved in the inactivation of ERK1/2 (24–26). The putative inhibition of Pyst1 by tungstate would potentially lead to an increased phosphorylation of its substrates, i.e. ERK1/2.
A first set of experiments was performed to compare the time-dependent dephosphorylation of ERK1/2 in tungstate-treated versus untreated cells. Briefly, CHO-R cells were treated with insulin for 10 min, the hormone was then removed, and the effect of tungstate on the phosphorylation state of ERK1/2 was evaluated by Western blot analysis at a range of time points. No significant differences were detected between treated and untreated cells (Fig. 3D). To further study this point, we performed a second set of experiments in which Pyst1 was overexpressed in 293T cells. Cells were treated with 100 nM epidermal growth factor in the presence or absence of 1 mM of tungstate, and the ERK1/2 phosphorylation state was then visualized by Western blot. Pyst1 overexpression enhanced the dephosphorylation of ERK1/2. This effect was not significantly blocked or delayed by preincubation with 1 mM tungstate (Fig. 3D). These results indicate that no MAPK phosphatase is involved in tungstate-dependent ERK1/2 activation.
Tungstate-induced Glycogen Deposition in Rat Primary Cultured Hepatocytes Is Dependent on ERK1/2 Activation—We tested whether tungstate-induced ERK1/2 phosphorylation was involved in the stimulation of glycogen deposition triggered by this compound. For this purpose, we used PD98059, a specific inhibitor of ERK1/2 phosphorylation. Incubation of CHO-R cells and hepatocytes with this inhibitor completely blocked the tungstate-induced phosphorylation of ERK1/2 (Fig. 4A). Under these conditions, PD98059 also prevented tungstate-induced glycogen deposition in cultured hepatocytes, indicating that this process is dependent on active ERK1/2 (Fig. 4B).
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Tungstate Treatment Leads to the Activation of p90rsk and Activation of Glycogen Synthesis in a PKB/Akt Phosphorylation-independent Way—The stimulation of glycogen synthesis by insulin is triggered by the phosphorylation of GSK3 at serine 9 and its concomitant inactivation. Therefore, we used specific antibodies to analyze whether tungstate treatment stimulates the phosphorylation of GSK3 in CHO-R and primary hepatocytes. Tungstate, like insulin, stimulated the phosphorylation of GSK3 at consensus serine 9 in both cell types, whereas no significant variations were observed in the total amount of this kinase (Fig. 5, A and E).
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GSK3 is mainly phosphorylated by activated PKB/Akt after insulin treatment (for a review, see Refs. 27 and 28). We analyzed whether tungstate stimulated the phosphorylation of PKB/Akt at consensus threonine 308 and serine 473 using antibodies specific for these phosphorylation sites. Our results show that, in contrast to insulin, this compound did not significantly modify the phosphorylation state of this kinase in CHO-R cells (Fig. 5B) or in primary cultured hepatocytes (not shown). Since PKB/Akt was not apparently involved in the phosphorylation of GSK3, we next checked whether ERK1/2 may play a role in this event. As shown in Fig. 5C, pretreatment of CHO-R cells with PD98059 abrogated tungstate-induced GSK3 phosphorylation. Similar results were obtained in primary cultured hepatocytes (not shown). p90rsk (MAPKAP-K1), one of the main substrates activated by phosphorylated ERK1/2, may be involved in the phosphorylation and inactivation of GSK3 (29–31). Tungstate treatment strongly stimulated the phosphorylation of p90rsk at consensus serine 380, both in CHO-R cells and in primary cultured hepatocytes, whereas no significant changes were observed in the total amount of this protein (Fig. 5, D and E). Therefore, the phosphorylation of p90rsk may be the link between ERK activation and GSK3 phosphorylation.
GSK3 inactivation may lead to the activation of GS, the key enzyme of glycogen synthesis. Incubation of primary cultured hepatocytes with tungstate increased the GS activation state (expressed as –glucose-6-phosphate/+glucose-6-phosphate activity ratio) in both soluble and particulate fractions (Fig. 6). Moreover, tungstate-induced GS activation was greatly reduced when the cells had been incubated previously with PD98059, indicating that an active ERK1/2 kinase is required for the activation of this enzyme (not shown).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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We considered the possibility that this compound may alter the phosphorylation of the IR -subunit, thus mimicking the action of insulin. Tungstate did not significantly alter the phosphorylation state of the IR -subunit, nor did it block or delay its dephosphorylation. Therefore, this compound bypassed the IR in its mechanism of action. In contrast, we observed a clear and transient activation of ERK1/2 when CHO-R cells or primary cultured hepatocytes were incubated with the compound. As a potential phosphatase inhibitor, tungstate might have kept ERK1/2 in an active state through inhibiting a MAPK phosphatase. However, this does not seem to be the case since dephosphorylation of ERK1/2 was not significantly delayed by tungstate. Furthermore, we have gained evidence that indicates that Pyst1/MKP3, the main phosphatase that acts on ERK1/2 (24–26), is not involved in tungstate-induced activation of these kinases.
The accepted mechanism by which insulin activates GS and therefore glycogen deposition is through the inactivation of GSK3 induced by phosphorylation at serine 9 (33–35). Tungstate treatment indeed promoted GSK3 phosphorylation at this consensus residue, which explains the observed activation of GS induced by this compound. Moreover, tungstate-induced GS activation and glycogen deposition were dependent on the activation of ERK1/2 since they were blocked by inhibition of the phosphorylation of these enzymes, indicating a connection between the phosphorylation of these kinases and glycogen synthesis in tungstate-treated cells. We propose that p90rsk links these two events as this protein is a substrate of ERK1/2 and is strongly activated in cells treated with tungstate and since it has been reported that it participates in the inactivation of GSK3 (29–31, 36–39).
Nevertheless, the role of p90rsk in the activation of GS is controversial. Some results show that in skeletal muscle and adipocytes, p90rsk is not significantly involved in the regulation of glycogen synthesis (40, 41). In fact, results from mice that lacked the RSK2 isoform showed that this protein is not required for activation of muscle GS by insulin but may indirectly modulate muscle GS activity and/or glycogen content (42). However, although muscle and adipose tissue mainly express the RSK2 isoform of p90rsk, a different isozyme, RSK1, is expressed in liver (43). Very little is known about RSK1 and its role in liver glycogen metabolism. In our experiments, RSK1 was stimulated by tungstate treatment in primary cultured hepatocytes, and on the basis of these results, we propose that RSK1 contributes to tungstate-induced GS activation and glycogen deposition in liver.
Surprisingly, all the phosphorylation events described were independent of the activation of PKB/Akt, the main contributor to GSK3 phosphorylation in the insulin signaling pathway. This is a remarkable result since it has been reported that the insulin- or IGF1-induced phosphorylation of GSK3 is prevented by inhibition of PKB/Akt but not of p70S6K or ERK1/2 (44).
Furthermore, tungstate-stimulated ERK1/2 activation is not correlated with an increase in the proliferation rate of CHO-R or other cell lines.2 Taken together, these results indicate that under tungstate stimulation, the metabolic effects of the activation of these MAPKs are prevalent over the mitogenic ones.
The question remains as to what activates ERK1/2 in this experimental situation. Our data indicate that these protein kinases are not directly activated by tungstate as they are sensitive to PD98059, a MEK1/2 inhibitor. We propose that tungstate acts on one or several of the components of the ras-raf-MEK-ERK pathway.
In conclusion, we have identified the first set of molecular targets through which sodium tungstate may exert its antidiabetic action. Moreover, this study provides novel data that point to ERK1/2 as a new target for the design of antidiabetic agents.
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FOOTNOTES |
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A recipient of a predoctoral fellowship from the Generalitat de Catalunya.
|| A recipient of a predoctoral fellowship from Ministerio de Educación, Cultura y Deporte.
|||| To whom correspondence should be addressed. Tel.: 34-93-403-71-60; Fax: 34-93-403-71-14; E-mail: guinovart{at}pcb.ub.es.
1 The abbreviations used are: IR, insulin receptor; IRS-1, insulin receptor substrate-1; ERK1/2, extracellular signal-regulated kinases 1 and 2; GS, glycogen synthase; GSK3, GS kinase-3; MAPK, mitogen-activated protein kinase; MEK, MAPK/ERK kinase; MAPKAP-K1, MAPK-activated protein kinase-1; PKB, protein kinase B; DMEM, Dulbecco's modified Eagle's medium; FCS, fetal calf serum; CHO, Chinese hamster ovary.
2 J. E. Domínguez, B. Nadal, R. Gomis, and J. J. Guinovart, manuscript in preparation.
3 A. Barberà, M. C. Muñoz, R. Gomis, and J. J. Guinovart, manuscript in preparation.
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ACKNOWLEDGMENTS |
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REFERENCES |
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