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Originally published In Press as doi:10.1074/jbc.M306941200 on September 23, 2003

J. Biol. Chem., Vol. 278, Issue 49, 49308-49315, December 5, 2003
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A Bimodal Modulation of the cAMP Pathway Is Involved in the Control of Myogenic Differentiation in L6 Cells*

Fabio Naro{ddagger}§, Vania De Arcangelis{ddagger}, Claudio Sette¶, Caterina Ambrosio||, Hiba Komati**, Mario Molinaro{ddagger}, Sergio Adamo{ddagger}, and Georges Nemoz**

From the {ddagger}Dipartimento di Istologia ed Embriologia Medica, Università "La Sapienza," 00161 Rome, Italy, **INSERM U585, Physiopathologie des Lipides et Membranes, INSA de Lyon, 69621 Villeurbanne, France, the Dipartimento di Sanità Pubblica e Biologia Cellulare, Sezione di Anatomia, Università "Tor Vergata," 00173 Rome, Italy, and the ||Dipartimento di Farmacologia, Istituto Superiore di Sanità, 00161 Rome, Italy

Received for publication, June 30, 2003 , and in revised form, September 22, 2003.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
We have previously shown that myogenesis induction by Arg8-vasopressin (AVP) in L6 rat myoblasts involves a sustained stimulation of type 4 cAMP-phosphodiesterase. In this model, we observed that a transient cAMP generation occurs in the minutes following AVP addition. Evidence suggests that cAMP generation is due to the prostaglandins produced in response to AVP binding to V1a receptors and subsequent activation of phospholipase A2. The early cAMP increase was effective in activating cAMP-dependent protein kinase (PKA) and increasing phosphorylation of CREB transcription factor. Inhibition of PKA by compound H89 prior to AVP addition led to a significant reduction of expression of the differentiation marker creatine kinase, whereas H89 added 1-5 h after AVP had no significant effect. Furthermore, PKA inhibition 24 h after the beginning of AVP treatment potentiated differentiation. This shows that both an early activation and a later down-regulation of the cAMP pathway are required for AVP induction of myogenesis. Because phosphodiesterase PDE4D3 overexpressed in L6 cells lost its ability to potentiate AVP-induced differentiation when mutated and rendered insensitive to PKA phosphorylation and activation, we hypothesize that the early cAMP increase is required to trigger the down-regulation of cAMP pathway through stimulation of phosphodiesterase.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Myogenic differentiation of myoblasts is a complex process resulting in the formation of multinucleated myotubes expressing an array of muscle-specific proteins. It takes place both in the course of normal embryonic development and during repair of lesions caused by muscle injuries or degeneration occurring in muscular dystrophies (1). Several hormonal factors have been shown to positively influence the myogenic process in vivo or in vitro. Among them, the neuropituitary hormone vasopressin (AVP)1 appears particularly interesting, because it induces myogenic differentiation of rat myoblast lines and mouse satellite cells in the absence of other factors (2). The signaling pathways triggered by AVP in L6-C5 myogenic cells include phospholipase C and phospholipase D activation (3, 4). We also reported that AVP-induced differentiation requires a prolonged activation of type 4 phosphodiesterase (PDE4) and a reduction of cAMP levels and PKA activity occurring after several hours of AVP treatment of myogenic cells (5), in agreement with studies showing that a sustained elevation of cAMP levels exerts a potent inhibitory effect on myogenic differentiation (6, 7). Phosphodiesterases of the PDE4 family, and especially the isoform PDE4D3, ensure a tight control of cAMP levels in L6-C5 cells and are positive effectors of the myogenic response (5, 8). However, the signaling cascades triggered by AVP in myoblasts are still largely unknown, and in particular, the delineation of the complex role played by the cAMP pathway remains incomplete. In fact, despite its recognized inhibitory effect on myogenic differentiation, it has been reported that cAMP intracellular levels and PKA activity rise at the onset of myogenesis (9-11), which suggests that the cAMP pathway may have a dual role in this process. Thus, it seems of importance to better understand the involvement of cAMP in the control of muscle formation. The recent description of alterations in the expression of genes involved in several signaling pathways, including the cAMP pathway, that have been noticed in muscular tissue of patients affected by dystrophic diseases (12) underscores the interest of this question.

In the present work, we describe early AVP effects on cAMP levels in L6-C5 cells and address the possible physiological meanings of the observed changes. Contrary to longer term observations, in the first few minutes of stimulation, AVP induced a marked and transient cAMP accumulation. We identified an indirect mechanism of cyclase stimulation by AVP in these cells involving prostaglandin synthesis, and we made observations indicating that although short-lived, the early cAMP surge has a physiological relevance and exerts a positive influence on the myogenic process. We hypothesize that an important effect of the early cAMP production is to trigger the activation of phosphodiesterase PDE4D3 resulting in the later down-regulation of the cAMP pathway.


   EXPERIMENTAL PROCEDURES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
RESULTS
 DISCUSSION
 REFERENCES
 
Materials—Synthetic AVP, [deamino-Pen1,O-Me-Tyr2,Arg8]-vasopressin V1a antagonist, Kemptide, PKA peptide inhibitor, a creatine kinase assay kit, and lipid standards were purchased from Sigma. Rolipram and H89 were obtained from Calbiochem-Merck KGAa. Fatty acid-free BSA and FuGene 6 were from Roche Applied Science. Anti-Phospho-CREB was from New England Biolabs (Beverly, MA). [{gamma}-32P]ATP, [3H]cAMP, [14C]arachidonic acid, and cAMP-[125I] radioimmunoassay kit were from PerkinElmer Life Sciences. The ECL Western blot detection kit was from Amersham Biosciences.

Cell Culture and Differentiation—Subcloning and characterization of L6 (13) rat myogenic cell clones was previously reported (3). The cells of the subclone C5 (L6-C5), a clone that had shown significant differentiation ability (2, 14), were used throughout this study. The cells were routinely seeded at the density of 10,000/cm2 in Dulbecco's modified Eagle's medium (DMEM) containing 4500 mg/ml glucose, supplemented with 100 units/ml penicillin, 100 µg/ml streptomycin, and 10% heat-inactivated FBS. Twenty-four hours after plating, the cultures were extensively washed with DMEM and shifted to serum-free medium consisting of DMEM supplemented with 1% (w/v) fatty acid-free BSA, with or without other additions. Full terminal myogenic differentiation was morphologically evaluated after 6 days by assessing the presence of multinucleated myotubes in May Grunwald-Giemsa stained cultures. To evaluate cell differentiation by quantification of specific markers, either the cells were homogenized in 30 mM HEPES, 1 mM EDTA, pH 7.2 buffer and creatine kinase activity was assessed as described in Ref. 2, or the cells were homogenized in RIPA buffer and myosin heavy chain content was evaluated by enzyme-linked immunosorbent assay, using MF20 as the primary antibody, as described in (5).

cAMP Assay—The cells cultured for 24 h in 1% BSA medium without addition were treated for different times with 10-6 M AVP in the presence or absence of various agents, as stated in figure legends. Before harvesting, the cells were washed twice with cold PBS, and 0.5 ml of ice-cold 10% trichloroacetic acid was added. The cell extracts were collected and centrifuged at 10,000 x g for 15 min. The supernatants were extracted five times with diethyl ether to eliminate trichloroacetic acid. cAMP was assayed by radioimmunoassay, according to the manufacturer's recommendations, using the acetylation procedure. The trichloroacetic acid pellets were used to quantify proteins by the Bradford microassay.

Reverse Transcriptase-PCR—Total RNA was prepared from quiescent L6-C5 myoblasts (i.e. cells cultured for 48 h in 1% BSA medium), myotubes (cells cultured for 6 d in the presence of 10-7 M AVP), or rat kidney or liver using Trizol reagent as advised by the manufacturer (Invitrogen). 5 µg of RNA from each sample was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and p(dT)10 primer (Roche Applied Science). The following primers, designed according to the nucleotide sequences of rat V1a vasopressin receptor cDNA (GenBankTM GI:16554454) and of rat V2 vasopressin receptor cDNA (GenBankTM GI:9506414) were used to amplify cDNAs: Pair 1 (V1aR nucleotides 1381-1530), sense 5'-AAATTCGCCAAGGATGACTC-3', and antisense 5'-TGGGGCTCAAGTGGAGACAG-3'; Pair 2 (V2R nucleotides 676-1116), sense 5'-GTTCTTATCTTCCGGGAGATAC-3', and antisense 5'-TCAGGAGGGTGTATCCTTCATC-3'. PCRs were carried out with 2.5 units/sample of platinum Taq polymerase (Invitrogen), by performing 27 amplification cycles of 94 °C for 30 s, 57 °C (for V1aR) or 54 °C (for V2R) for 40 s, and 68 °C for 50 s. Integrity and equal cDNA loading in the PCR was checked by quantification of {beta}-actin mRNA levels, by using primers designed according to mouse cytoskeletal {beta}-actin cDNA sequence (GI:49865), to obtain a 355-bp band (sense, nucleotides 309-330, 5'-ACCAACTGGGACGACATGGAG-3'; and antisense, nucleotides 693-663, 5'-GGTCAGGATCTTCATGAGGTAGTC-3'). The PCR products were analyzed on a 2% agarose gel.

Transfection Experiments—Transient transfections were performed by using FuGene 6TM according to the manufacturer's instructions (Roche Applied Science). Briefly, the pCDNA3-V1aR construct or empty vector (1 µg each in a total volume of 20 µl in Dulbecco's modified Eagle's medium) were mixed with diluted FuGene reagent (97 µl of serum-free medium with 3 µl of FuGene) and incubated for 15 min. The DNA-FuGene mix was then added dropwise to 200,000 COS-1 cells/ml in suspension. The transfected cells were seeded and incubated for an additional 48 h in 10% FBS-containing DMEM. They were then switched to BSA medium and received 0.5 mM IBMX and, after 10 min, 10-6 M AVP. After 15 min of incubation at 37 °C, the cell extracts were prepared and assayed for cAMP content as described above. L6-C5 cells were transiently transfected with either pCMV5-PDE4D3 construct, pCMV5-S54A-PDE4D3 (15), or the empty vector, in the same conditions as above. After 16 h of culture in FBS medium, they were shifted to BSA medium and cultured for 6 h in the presence or absence of 10-7 M AVP. Myogenic differentiation was evaluated by assessing the nuclear myogenin content of the cells by immunofluorescence. The cells were fixed in 4% paraformaldehyde in PBS for 30 min at 4 °C and permeabilized in 0.2% Triton X-100 in PBS for 30 min. They were washed with 1% BSA in PBS and incubated overnight at room temperature with the undiluted supernatant of F5D hybridoma cells (developed by Dr. W. E. Wright, University of Texas, Dallas, obtained from the Developmental Studies Hybridoma Bank maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA). After washing with 1% BSA in PBS, the cells were incubated for 1 h at room temperature with rhodamin-conjugated goat anti-mouse IgG (Cappel Laboratories, West Chester, PA) and examined by fluorescence microscopy.

Analysis of Arachidonate Metabolism—The cells cultured for 24 h in 1% BSA medium were incubated for 2 h in the presence of 0.5 µCi/ml of [14C]arachidonic acid dissolved in DMEM without FBS or BSA. Radioactivity of the culture medium was then measured to assess the incorporation of the fatty acid (usually 80-90%). The cells were washed with 0.5% BSA in DMEM, equilibrated for 15 min in the same medium, and treated for 5 min with 10-7 M AVP at 37 °C. The radioactivity of the medium was then determined on aliquots to evaluate the amount of arachidonate and metabolites released. The remainder was acidified to pH 3-4 with HCl and extracted two times by 3 volumes of ethyl acetate containing 50 µM butyl-hydroxytoluene. Pooled organic phases were evaporated under nitrogen stream, and the lipid extract was analyzed by thin layer chromatography on a Silica gel plate (Merck) with water-saturated ethyl acetate, iso-octane, acetic acid (110:50:20) as eluent. The dry plate was treated by iodine vapors to detect lipid standards and then autoradiographed for 6 weeks at -75 °C using Hyperfilm MP (Amersham Biosciences).

Protein Kinase A Activity—Protein kinase A activity was measured by evaluating the incorporation of labeled phosphate from [{gamma}-32P]ATP into the synthetic peptide substrate Kemptide, as described in Ref. 5. cAMP-Phosphodiesterase Activity—cAMP-phosphodiesterase activity was assayed by a two-step radioisotopic procedure, based on the transformation of labeled 5'-AMP produced by phosphodiesterase action on [3H]cyclic AMP into adenosine by treatment with 5'-nucleotidase and separation of labeled substrate and adenosine end product by anion exchange column chromatography, as described in Ref. 5.

Western Blot Analysis—Cells cultured in 1% BSA medium for 24 h were treated with AVP for different times, washed, and directly extracted in Laemmli buffer. The extracts were probe-sonicated for 2 s. 20 µg of protein extracts were analyzed by SDS-PAGE. Immunodetection was performed with a polyclonal anti-phospho-CREB antibody (New England Biolabs) diluted 1:1000. Second antibody incubation was carried out with 1:10,000 dilution of anti-rabbit-IgG antibody conjugated to horseradish peroxidase. Immunostained bands were detected by the ECL method (Amersham Biosciences).


   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
DISCUSSION
 REFERENCES
 
AVP Induces a Transient Increase in cAMP Levels—Because intracellular cAMP concentration plays a crucial role for myogenic differentiation, we set out to investigate in detail the modulation of cAMP levels by AVP, a strong inducer of differentiation in L6-C5 cells. We found that AVP stimulation of L6-C5 cells provoked a transient increase in the intracellular cAMP concentration, with a peak reached after 1-3 min and a return to basal levels after ~15 min (Fig. 1A). The maximal AVP-induced cAMP accumulation measured in the presence of the nonselective phosphodiesterase inhibitor IBMX was about 10-20-fold the basal level, much lower than that induced by epinephrin in the same conditions (almost 1000-fold basal level; Fig. 1B). AVP elicited a concentration-dependent increase of cAMP levels, with an EC50 of 0.3 nM (Fig. 1C), a concentration within the range of the physiological concentrations of the hormone in the bloodstream.



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  		FIG. 1.
AVP induces an increase of cAMP levels in L6-C5 cells. A, transient increase of cAMP level after AVP treatment. Intracellular cAMP concentration was measured after incubation of the cells with or without 1 µM AVP for the indicated times. The cells were treated at different times, and all of the samples (control (•) and AVP-treated ({circ})) were harvested and assayed at the same time. The results are the means ± S.D. of triplicates and are representative of four experiments. Significant changes are indicated by * (p < 0.05) and ** (p < 0.01). B, comparison of cAMP variations in response to epinephrine and AVP. The cells were pretreated for 10 min with 0.5 mM IBMX, and then 10-6 M AVP or 10-6 epinephrine were added for an additional 10 min before cAMP level measurement. The results are the means ± S.D. of three independent measurements performed in triplicate. C, dose dependence of AVP-induced cAMP increase. Cells pretreated for 10 min with 5 mM IBMX were exposed for 10 min to increasing concentrations of AVP. Inset, cells were incubated with 10-7 M AVP in the presence or absence of 10-5 M of the V1aR antagonist [deamino-Pen1,O-Me-Tyr2,Arg8]-vasopressin. The results are the means ± S.D. of three independent measurements performed in triplicate. D, effect of PDE4 inhibition on AVP-induced cAMP increase. The cells were incubated with 10-5 M rolipram or solvent for 10 min before being challenged with 10-6 M AVP for the indicated times. The assay was performed in triplicate.

 
Type 4 Phosphodiesterase Activity Is Involved in the Limitation of Amplitude and Duration of the Early cAMP Response to AVP—We have previously shown that AVP triggers a biphasic activation of type 4 cAMP-specific phosphodiesterase, with an initial peak after 2 min and a later one after 15 min of hormonal treatment (5). To determine whether the transient nature of the cAMP rise triggered by AVP was due to the activity of this phosphodiesterase family, cAMP levels were measured in L6-C5 cells stimulated by AVP in the presence or absence of rolipram, a selective PDE4 inhibitor. Interestingly, we observed that rolipram strongly potentiated the increase in cAMP concentration caused by AVP treatment and allowed the maintenance of elevated cAMP levels for at least 30 min (Fig. 1D). This result indicates that PDE4 activity is required for the rapid return of cAMP to basal levels observed in L6-C5 cells and suggests that AVP controls the level and duration of the cAMP transient by concomitantly stimulating cAMP production and degradation by PDE4 in these cells.

The AVP-induced cAMP Accumulation Is Mediated by V1a Receptor Stimulation—It has been suggested, on the basis of functional observations, that L6 myoblasts do not express the adenylyl cyclase-coupled V2 receptors for AVP. On the other hand, these cells express V1 receptors, which are connected to the phospholipase C signaling pathway (16). We set out to determine whether low levels of the V2 receptor, which could account for the cAMP response to AVP, are expressed by L6-C5 cells. We thus performed reverse transcriptase-PCR experiments using primers designed to amplify specific regions of the V1a and V2 receptor cDNAs. As shown in Fig. 2A, V1a receptor mRNA transcripts are present in both L6-C5 myoblasts and differentiated myotubes. However, we failed to detect even trace amounts of V2 receptor mRNA (Fig. 2A), indicating that AVP does not act through this adenylyl cyclase-coupled receptor in L6-C5 cells.



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  		FIG. 2.
V1a receptor mediates the AVP-induced cAMP accumulation. A, RNA from mouse kidney and liver, L6-C5 myoblasts (Mb), and myotubes (Mt) was reverse transcribed, and PCRs were run as described under "Experimental Procedures," with primer pairs specific, respectively, for the V1a and V2 receptors and {beta}-actin for assessment of mRNA integrity. The expected size for the positive reaction was 150 bp for V1a receptor and 441 bp for V2 receptor. This experiment was repeated twice with similar results. B, COS cells were transiently transfected with either the pCDNA3 expression vector containing V1a receptor cDNA or the empty vector (MOCK) and plated in 10% FBS-containing medium. After 48 h, the cells were switched to 1% BSA medium, preincubated with 0.5 mM IBMX for 10 min, and stimulated with 10-6 M AVP for another 15 min. Intracellular cAMP was then measured as described under "Experimental Procedures." The results are the means ± S.D. of three independent experiments performed in triplicate. The average cAMP level in the mock transfected control cells was 12.0 ± 1.4 pmol/mg of protein. **, significantly different from all the other conditions (p <= 0.005).

 
To test whether the V1 receptor was involved in cAMP generation triggered by AVP, L6-C5 cells were stimulated in the presence of an excess of the selective V1 receptor antagonist [deamino-Pen1,O-Me-Tyr2, Arg8]-vasopressin and cAMP accumulation was measured. We observed that the V1 receptor antagonist completely suppressed the rise in intracellular cAMP concentration induced by AVP in L6-C5 cells (Fig. 1C), indicating that the hormone action requires stimulation of V1 receptors.

To ascertain whether activation of V1a receptors is sufficient to mediate the AVP-induced cAMP rise, we transiently transfected COS-1 cells, which do not respond to AVP, with an expression plasmid carrying the V1a receptor cDNA, and we evaluated the cAMP response of transfected cells. As expected, AVP stimulation did not exert any effect on cAMP levels in mock transfected cells. By contrast, transfection of V1a receptor was sufficient to confer to COS cells the ability to respond to AVP, a 70% increase in intracellular cAMP levels being observed after hormonal stimulation (Fig. 2B).

The mechanism of cAMP Synthesis Activation Induced by AVP in L6 Cells Involves Prostaglandin Synthesis and Secretion—Because V1a receptor is not coupled to adenylyl cyclase, we hypothesized that the activation of cAMP synthesis was due to an indirect mechanism set in motion by AVP. It has been reported that AVP triggers phospholipase A2 activation and liberation of free arachidonic acid in H9c2 cardiac myoblasts (17). Considering that free arachidonate may be further metabolized into prostaglandins that are known activators of cAMP synthesis, we tested the existence of such a pathway in L6-C5 cells. We first determined whether AVP was able to trigger the synthesis of prostaglandins in these cells. L6-C5 myoblasts were labeled by preincubation with [14C]arachidonic acid before the stimulation with the hormone. We observed that a 5-min AVP treatment lead to a 2-fold increase in the total radioactivity released in the culture medium (not shown), indicative of phospholipase A2 stimulation. TLC analysis of lipid extracts of the culture medium (Fig. 3A) showed that AVP promoted the synthesis and release of prostaglandins E2 and D2 principally, and PGF2{alpha} in smaller amount, suggesting that these compounds could act as autocrine effectors to activate adenylyl cyclase. To examine this hypothesis, we measured cAMP accumulation in response to AVP in the presence of two different cyclooxygenase inhibitors able to block the conversion of endogenous arachidonate into prostaglandins. Indeed, the cAMP increase induced by AVP was completely suppressed by indomethacin and partially by aspirin (Fig. 3B), confirming that prostaglandin synthesis was required for the production of cAMP.



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  		FIG. 3.
AVP induces cAMP production in L6-C5 cells through the release of prostaglandins. A, prostaglandins are synthesized under the action of AVP. The cells were labeled with [14C]arachidonic acid and treated (lane b) or not (lane a) for 5 min with 10-7 M AVP. Lipid extracts from the cell supernatants were analyzed by thin layer chromatography and autoradiography. Lipid standards were run in parallel. B, inhibition of prostaglandin synthesis suppresses the AVP-induced cAMP increase. The cells were preincubated for 15 min with 0.5 mM IBMX in the presence or absence of 10-5 M indomethacin or 0.2 mM aspirin and then treated for 15 min with 10-7 M AVP, before intracellular cAMP measurement. The results are the means ± S.E. of six different samples for controls and three different samples for the other points, each sample being assayed in triplicate. **, significantly different from control with AVP (p = 0.003). ***, p < 0.001.

 
AVP Induces a Rapid Activation of the cAMP Signaling Pathway—To assess whether, despite its transient feature, the AVP-induced cAMP elevation has a functional significance, we investigated the consequences of AVP treatment on the downstream steps of the cAMP pathway of signaling. We found that PKA was activated 2.3-fold in L6 cells treated for 5 min with AVP and that PKA activity returned to basal levels in 30-45 min (Fig. 4A). Furthermore, as shown in Fig. 4B, AVP stimulation resulted in the phosphorylation of the CREB transcription factor, the PKA phosphorylation of which represents a critical step in the transcriptional regulation of gene expression by the cAMP pathway (18). CREB was rapidly phosphorylated in response to AVP, with a steady-state phosphorylation reached in 15-30 min (Fig. 4B, upper panel). In the presence of the PKA inhibitor H89, AVP-induced CREB phosphorylation was totally suppressed (Fig. 4B, lower panel), suggesting that it was mediated by activated PKA.



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  		FIG. 4.
AVP activates the cAMP pathway in L6-C5 cells. A, time course of PKA activation by 1 µM AVP. Each point is the mean ± S.D. of four independent experiments. B, time course of CREB phosphorylation induced by AVP. The cells were extracted after different times of treatment by 10-7 M AVP, and the proteins were immunoblotted with an antibody specific for phosphorylated CREB. Lower panel, PKA inhibition by H89 suppresses CREB phosphorylation induced by AVP. The cells were pretreated or not for 15 min with 20 µM H89 and then treated at different times with 10-7 M AVP. All of the samples were harvested at the same time and analyzed as above. The experiment was repeated twice with similar results.

 
The time course of PKA activation by AVP (Fig. 4A) indicates that its maximal activity is reached before the second phase of PDE4 activation, which peaks at 15-30 min (5). Because the major type 4 phosphodiesterase isoform expressed in L6 cells is PDE4D3, which can be activated by PKA-mediated phosphorylation (15), it is possible that the late phase of PDE stimulation depends on PKA. Thus, we tested the effects of PKA inhibition on the biphasic activation of PDE by AVP. As shown in Fig. 5, only the late activation of PDE (15 min) was dependent on PKA activity, whereas the early activation (3 min) was not affected in the presence of the PKA inhibitor H89. This result indicates that PKA is involved in the second phase of AVP-dependent PDE stimulation.



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  		FIG. 5.
Effect of PKA inhibition on the AVP-induced PDE activation in L6-C5 cells. A, effect of H89 on the first phase of PDE activation by AVP. The cells were pretreated or not by 10-5 M H89 for 15 min and treated by 10-7 M AVP for 3 min. They were then immediately washed, homogenized, and assayed for PDE activity. B, effect of H89 on the second phase of PDE activation by AVP. The cells were treated as above, except that AVP treatment lasted for 15 min. The results are the means ± S.E. of four to six samples from two experiments, each being assayed in triplicate. *, different from control (p = 0.02). **, p = 0.009. ***, p = 0.001. #, different from AVP alone (p = 0.006).

 
The Transient Activation of the cAMP Pathway Induced by AVP Positively Influences Myogenic Differentiation—To evaluate whether the early activation of the cAMP pathway is required for L6-C5 cell myogenic differentiation triggered by AVP, we added H89 at different times before or after the onset of AVP treatment. When PKA activity was blocked before the AVP treatment, it resulted in a marked decrease in the number and the size of myotubes formed after 6 days in response to AVP (Fig. 6A). This effect was confirmed by measuring creatine kinase activity, a marker of terminal differentiation (Fig. 6B). H89 added before the onset of AVP stimulation significantly decreased creatine kinase expression (by 65% when added 90 min before AVP), which suggests that early PKA-mediated events have a positive influence on myogenic differentiation. Interestingly, the H89 inhibition of creatine kinase expression was not significant if the inhibitor was added 1-5 h after the onset of AVP treatment (Fig. 6B), demonstrating that a short-lived activation (within 1 h) of the cyclic AMP pathway is sufficient to stimulate myogenic differentiation. Furthermore, we observed that inhibition of PKA activity by H89 24 h after the onset of AVP treatment induced a significant stimulation of terminal differentiation, as evaluated by measuring after 6 days the activity of creatine kinase (+71% with H89 treatment) and the expression of myosin heavy chain (+96% with H89 treatment) (Fig. 6C).



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  		FIG. 6.
Effect of PKA inhibition on the AVP-induced myogenic differentiation of L6-C5 cells. A, morphological examination of the effects of H89 pretreatment on myotube formation induced by AVP. The cells cultured in 1% BSA medium were pretreated (panels c and d) or not (panels a and b) with 10 µM H89 for 20 min and then stimulated (panels b and d) or not (panels a and c) with 10-7 M AVP. After 6 days of culture, the cells were stained, and the formation of myotubes was assessed by microscopy. B, the effect of H89 on myogenic differentiation depends on the time of inhibitor addition. The cells cultured in 1% BSA medium were treated by 5 µM H89 at different times before or after the addition of 10-7 M AVP. After 6 days of culture, the cells were harvested, and creatine kinase activity was assayed. The results are the means ± S.E. of five to eleven independent measurements performed in triplicate. *, significantly different from control with AVP (p < 0.01). {dagger}, significantly different from H89 added 90 min before AVP (p < 0.01). C, H89 added 24 h after AVP potentiates AVP differentiating effect. The cells, treated or not by AVP as above, received 5 µM H89 24 h later. After 6 days of culture, the cells were harvested, and creatine kinase activity was assayed, or myosin content was evaluated by enzyme-linked immunosorbent assay. The results are expressed as percentages of the value of control with AVP and are the means ± S.E. of nine samples, each assayed in triplicate. *, significantly different from the control with AVP (p < 0.01).

 
A Mutated Form of Phosphodiesterase PDE4D3 That Is Insensitive to PKA Phosphorylation Is Less Efficient at Enhancing AVP-induced Differentiation than Wild-type Enzyme—We have previously shown that overexpression of the PDE4D3 phosphodiesterase isoform is able to potentiate AVP-induced differentiation of L6-C5 cells and, in particular, to enhance the nuclear accumulation of myogenin, an early and crucial step of the myogenic process (5). Based on the results of Fig. 5 suggesting that AVP induces a PKA-mediated activation of phosphodiesterase, it is possible that PDE4D3 phosphorylation is involved in AVP differentiating effects. To test the requirement of PKA-mediated phosphorylation and activation of PDE4D3 for the onset of myogenic differentiation, we took advantage of a PDE4D3 point mutant, S54A-PDE4D3, which is insensitive to PKA activation (15). L6-C5 cells were transfected with either wild-type or S54A-PDE4D3, stimulated with AVP, and monitored for nuclear translocation of myogenin. As shown in Fig. 7, the PKA-insensitive mutant, in contrast with the wild-type form, did not activate the basal level of differentiation, nor did it enhance the effect of AVP, suggesting that PKA-dependent phosphorylation of PDE4D3 has an important role in the AVP-induced myogenic response.



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  		FIG. 7.
A PKA-insensitive mutant of the PDE4D3 phosphodiesterase isoform is less efficient than the wild-type counterpart at enhancing the AVP-induced nuclear accumulation of myogenin. A, analysis of myogenin nuclear accumulation by immunofluorescence in PDE-transfected L6-C5 cells. L6-C5 myoblasts were transiently transfected either with pCMV5 containing wild-type PDE4D3 cDNA, pCMV5 containing mutant S54A-PDE4D3 cDNA (15), or the empty vector (mock). After 16 h, the cells were shifted to 1% BSA medium with or without the addition of 10-7 M AVP. After 6 h, myogenin was detected by immunofluorescence, by using the anti-myogenin antibody F5D and a secondary antibody coupled to rhodamin. All the nuclei in presence were visualized by UV microscopy using Hoechst 33342 nuclear dye. ser54ala mut., S54A-PDE4D3. B, quantification of myogenin positive nuclei in the above experiment. The results are expressed as percentages of myogenin-positive nuclei. Normalization of the efficiency of transfection was obtained by cotransfecting a GFP-carrying plasmid. The results are the means ± S.E. of 10 fields counted in a typical experiment, representative of four performed. The basal specific activities of PDE in the homogenates of cells transfected with wild-type PDE4D3 and S54A mutant were very similar (5-6-fold the activity of mock transfected cell homogenates). *, different from mock without AVP (p < 0.05). ***, different from mock with AVP (p < 0.001). ###, different from wt-PDE4D3 transfected with AVP (p < 0.001).

 

   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
REFERENCES
 
We have previously established that the neuropituitary hormone AVP is able to strongly stimulate the in vitro myogenic differentiation of L6 myoblasts, as well as of other myogenic cell lines and primary cells. AVP myogenic effects involve the sustained activation of a type 4 phosphodiesterase and a prolonged decrease in both cAMP levels and PKA activity, occurring after a few hours of AVP treatment. In the present study, we examined the modifications of the cAMP pathway induced by AVP in L6 cells in the first minutes of the treatment. We found that, in contrast to what is observed in longer term studies, AVP produced an elevation of cAMP levels, which peaked at 1-3 min and then rapidly returned to basal levels.

Activation of the cAMP pathway by AVP classically involves the stimulation of V2 receptors linked to the activation of adenylyl cyclase through interaction with the Gs protein, as observed for example in kidney collecting duct (19). However, functional observations suggest that L6 myoblasts only express the V1 receptors for AVP, which are related to phospholipase C activation. We have used the reverse transcriptase-PCR approach to clearly define the expression profile of AVP receptors in L6 cells, and we observed that only V1a receptor mRNA transcripts are detectable in L6 myoblasts, as well as in differentiated myotubes. That AVP is able to indirectly stimulate adenylyl cyclase through the activation of V1 receptors was confirmed by showing that a selective V1 receptor antagonist inhibited the AVP-induced cAMP accumulation. Furthermore, we observed that overexpression of V1a receptor in COS-1 cells confers to these cells the ability to respond to AVP stimulation by an increase in cAMP, which demonstrated that a signaling pathway triggered by V1a receptor stimulation indirectly causes the activation of adenylyl cyclase.

The indirect mechanism of cyclase stimulation involves prostaglandin synthesis, because it was inhibited by two prostaglandin synthesis inhibitors, indomethacin and aspirin. This is consistent with our observation of a stimulation of arachidonate release and synthesis of prostaglandins E2, D2, and F2{alpha} under AVP stimulation of L6 myoblasts. Arachidonate release has been reported to occur in AVP-treated cardiac myoblasts through the stimulation of V1 receptors (17), and AVP-induced prostaglandin synthesis in vascular smooth muscle cells has also been reported (20). As determined in the H9c2 cardiac myoblasts, the cascade leading to AVP-induced arachidonate release includes the following steps: AVP binding to V1 receptors triggers the activation of phospholipase C and leads to increased intracellular Ca2+, mitogen-activated protein kinase activation, and cPLA2 phosphorylation. In L6 skeletal myoblasts, a similar activation of mitogen-activated protein kinases by AVP has been observed.2 In these cells, free arachidonate could then ultimately be metabolized into prostaglandins E2, D2, and secondarily F2{alpha}, which, after release into the extracellular medium, could activate adenylyl cyclase through binding to their receptors. Despite the multiplicity of the steps involved in this process, it can likely take place in a short time span, as shown in other systems. It has been reported that in tracheal smooth muscle cells bradykinin induces cAMP synthesis through a similar pathway, the maximal cAMP concentration being reached in 2 min (21, 22).

Although transient and of modest amplitude compared with the one elicited by epinephrine, the AVP-induced cAMP increase was able to trigger the activation of downstream effectors, as evidenced by PKA activation and H89-sensitive CREB transcription factor phosphorylation. Because H89 is known not to be a fully specific PKA inhibitor (23), we cannot exclude that other kinases, together with PKA, are involved in AVP-induced CREB phosphorylation. Nevertheless, this last observation suggests that the early steps of the myogenic process induced by AVP could include the expression of cAMP-regulated genes. In addition, activation of PKA seems to be involved in the delayed phase of PDE4 stimulation by AVP, as shown by its marked reduction under PKA inhibition.

The essential role of early PKA activation in the AVP-induced differentiation process is supported by experiments showing that differentiation is impaired if PKA activity is inhibited by H89 added before the differentiating agent AVP. Because we have shown in a previous study that the AVP-induced differentiation process requires an elevation of cAMP-phosphodiesterase activity resulting in a prolonged lowering of cAMP levels and PKA activity (from 3 to 48 h after AVP addition), the above observations might appear contradictory. However, in view of the transitory feature of the cAMP pathway activation triggered by AVP, a model reconciling our different observations can be proposed. In the first period of L6 response, an elevated PKA activity would have a positive influence on initiation of the myogenic process. Then, the progress of differentiation would require a rapid deactivation of the cAMP pathway insured by cAMP-PDE stimulation, a sustained high level of cAMP being strongly inhibitory to further differentiation steps. This notion is in agreement with the present observation that the PKA inhibitor H89 added 24 h after the onset of AVP treatment stimulated myogenesis. It can be noticed that opposite effects were obtained under treatment by a cAMP-elevating agent. The addition of rolipram to the culture medium up to 24 h after AVP induced a complete inhibition of differentiation (5), later treatments being ineffective,2 which supports the conclusion that H89 effects at 24 h were actually due to PKA inhibition. It is thus likely that molecular event(s) negatively regulated by cAMP and taking place at 24 h is (are) responsible for the cAMP-induced blockade of myogenesis. We can hypothesize that one such event is the nuclear accumulation of the muscle-specific transcription factor myogenin, because we have previously shown that it is totally inhibited if cAMP levels are kept elevated by the presence of the phosphodiesterase inhibitor rolipram (5, 8).

Such a model implying a bimodal modulation of the cAMP pathway allows integrating apparently conflicting results obtained with different myogenic cell systems. First, it is consistent with data demonstrating that maintaining high cAMP levels throughout the experiment or overexpressing PKA catalytic subunit inhibits the differentiation of mouse and rat myoblast lines (7, 24). This model also takes into account the data showing that increasing cAMP before the onset of differentiation (i.e. before switching cells to differentiating conditions) induces a potent stimulation of avian myoblast terminal differentiation (10). In this last study, the authors observed an amplifying effect of triiodothyronin on a transient elevation of cAMP levels preceding the onset of myoblast fusion. They propose that at least part of the positive effect of triiodothyronin on quail myoblasts terminal differentiation is mediated by cAMP, through enhanced withdrawal of the cells from the replicative state, because both triiodothyronin and cAMP inhibit avian myoblast proliferation. However, this hormone does not influence proliferation in a murine myoblast model (25), and cAMP has been shown to have no influence on the proliferation of a variety of myogenic cells of mammalian origin (24, 26-28). In our hands, the cAMP-elevating agents rolipram and forskolin had very little effect on L6-C5 cell proliferation.2 It is thus likely that in the L6-C5 rat myoblasts the positive effect of cAMP on the myogenic response does not involve an accelerated withdrawal from the cell cycle. cAMP effectors such as PKA and CREB might target a still undefined element of the complex machinery, which insures the control of skeletal muscle-specific gene expression by the Myo D family of transcription factors. In C2 mouse myoblasts, phosphorylated CREB is increased at the onset of differentiation and associates with MyoD and other protein partners in a complex targeting a cyclic AMP-responsive element in the promoter of RB gene, which results in an enhancement of RB expression, RB protein being involved not only in cell cycle arrest but also in the expression of late stage muscle-specific genes and in prevention of apoptotic cell death during differentiation (29).

Additionally, it is possible that moderate and transient early activation of the cAMP pathway is required to induce a later inactivation of this pathway, through PKA phosphorylation and activation of the type 4 phosphodiesterase PDE4D3, which is in agreement with our observation that PKA inhibition reduces the increase in phosphodiesterase activity induced by a 15-min AVP stimulation. This would ensure the prolonged lowering of cAMP levels that is essential to the progress of myogenesis. In strong support to this hypothesis, we observed (Fig. 7) that overexpression in L6 cells of a mutated PDE4D3 phosphodiesterase isoform lacking the serine 54 target of PKA phosphorylation was much less effective than overexpression of the wild-type enzyme at enhancing the AVP-induced nuclear import of myogenin, a crucial step of the myogenic process. Thus, PKA-dependent phosphorylation and activation of PDE4D3 seem to play important roles in the progress of AVP-induced myogenesis. In this regard, the recently reported dramatic underexpression of the PDE4D gene, that encodes phosphodiesterase PDE4D3, in the muscle of patients affected by Duchenne muscular dystrophy (12), illustrates the involvement of this enzyme in the preservation of muscle function and stresses the importance of the control of cAMP signaling by phosphodiesterase in muscle pathophysiology.

The present study supports the existence of an autocrine loop responsible for a bimodal modulation of the cAMP pathway and its physiological involvement in myogenesis. This autocrine loop involves prostaglandin formation, which underlines the role of arachidonic acid metabolism in muscle differentiation. Such a role of prostanoids had been proposed in early pioneer work (30). More recently, the importance of the inflammatory component in muscular dystrophy pathogenesis has been evidenced by gene expression studies that pointed out, in particular, an overexpression of phospholipase A2 likely to increase prostanoid content in human dystrophic muscle (12, 31). In addition, it has been recently reported that PGF2{alpha} stimulates muscle cell growth and nuclear accretion by increasing intracellular calcium concentration (32). The delineation of the role of prostaglandins in the formation and regeneration of muscle thus appears to deserve further investigation.


   FOOTNOTES
 
* This work was supported by grants from the Ministero Istruzione Università e Ricerca (to S. A. and M. M.), from University of Rome-La Sapienza (to S. A. and F. N.), and from Centro di Eccellenza Biologia e Medicina Molecolare. The exchanges between the collaborating institutions were supported by a Consiglio Nazionale delle Ricerche-Institut National de la Santé et de la Recherche Médicale joint program grant (to G. N. and S. A.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

§ To whom correspondence should be addressed: Dipartimento di Istologia ed Embriologia Medica, Università di Roma La Sapienza, 00161 Rome, Italy. Tel.: 39-06-4976-6587; Fax: 39-06-4462-854; E-mail: fabio.naro{at}uniroma1.it.

1 The abbreviations used are: AVP, Arg8-vasopressin; DMEM, Dulbecco's modified Eagle's medium; PDE, cAMP-phosphodiesterase; PKA, cAMP-dependent protein kinase; rolipram, 4-(3-[cyclopentyloxy] 4-methoxyphenyl)-2-pyrrolidinone; BSA, bovine serum albumin; FBS, fetal bovine serum; IBMX, isobutylmethylxanthine; PBS, phosphate-buffered saline; CREB, cAMP-responsive element-binding protein. Back

2 F. Naro, V. De Arcangelis, C. Sette, H. Komati, S. Adamo, and G. Nemoz, unpublished results. Back


   ACKNOWLEDGMENTS
 
We thank Dr. Tommaso Costa for helpful discussion and critical reading of the manuscript and Dr. S. J. Lolait for kind sharing of the V1aR carrying plasmid.



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 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
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