HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 279, Issue 10, 8648-8654, March 5, 2004

This Article Abstract Full Text (PDF) All Versions of this Article: 279/10/8648    most recent M312095200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cheng, B. Articles by Tse-Dinh, Y.-C. Search for Related Content PubMed PubMed Citation Articles by Cheng, B. Articles by Tse-Dinh, Y.-C. Social Bookmarking                      What's this?

Flexibility at Gly-194 Is Required for DNA Cleavage and Relaxation Activity of Escherichia coli DNA Topoisomerase I^*

Bokun Cheng, Jingyang Feng, Sharvari Gadgil, and Yuk-Ching Tse-Dinh

From the Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595

Received for publication, November 4, 2003 , and in revised form, January 1, 2004.

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
The proposed mechanism of type IA DNA topoisomerase I includes conformational changes by the single enzyme polypeptide to allow binding of the G strand of the DNA substrate at the active site, and the opening or closing of the "gate" created on the G strand of DNA to the passing single or double DNA strand(s) through the cleaved G strand DNA. The shifting of an helix upon G strand DNA binding has been observed from the comparison of the type IA DNA topoisomerase crystal structures (Changela, A., DiGate, R. J., and Mondragón, A. (2001) Nature 411, 1077–1081). Site-directed mutagenesis of the strictly conserved Gly-194 at the N terminus of this helix in Escherichia coli DNA topoisomerase I showed that flexibility around this glycine residue is required for DNA cleavage and relaxation activity and supports a functional role for this hinge region in the enzyme conformational change.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
DNA topoisomerases are ubiquitous enzymes involved in DNA replication, transcription, and recombination (reviewed in Refs. 1–3). These enzymes catalyze the interconversion of DNA topological isomers by first cleaving one or more DNA strands and then allowing another single- or double-stranded DNA strand to pass through the cleaved DNA. After religation of the cleaved DNA, the DNA involved in strand passage has to be released from the enzyme molecule. There are therefore multiple steps in catalysis that would require changes in enzyme conformations. Type IA DNA topoisomerases catalyze the topological conversions by breaking and rejoining a single strand of DNA with the cleaved DNA forming a 5'-phosphoryltyrosine linkage with the enzyme. The current models of catalysis for this class of DNA topoisomerases (4–6) begin with the binding of the enzyme at the junction of single- and double-stranded DNA, followed by cleavage of a single G strand of DNA by the active-site tyrosine to create a "gate" for subsequent strand passage. However, the crystal structures of the enzymes in the absence of DNA (7–9) show insufficient space around the active-site tyrosine responsible for G strand DNA cleavage to accommodate the placement of the G strand of DNA in the vicinity of the tyrosine hydroxyl nucleophile. A more recent crystal structure of Escherichia coli DNA topoisomerase III with an 8-base-long single-stranded oligonucleotide bound at the active site showed that an helix making contacts with the G strand DNA has shifted relative to its position in the structure of E. coli DNA topoisomerase III with no DNA bound (10). The corresponding helix in E. coli DNA topoisomerase I begins at position 195 (Fig. 1). The glycine residue preceding the N terminus of this helix (Gly-194) is strictly conserved among type IA DNA topoisomerases (11). The side chains of residues equivalent to Arg-195 and Gln-197 of E. coli DNA topoisomerase I make contacts with the phosphate backbone in the topoisomerase III oligonucleotide crystal structure (10). We hypothesized that the conformational flexibility around the Gly-194 of E. coli DNA topoisomerase I may be important for the movement of this helix within the enzyme during catalysis so that the active-site tyrosine will be in a position to carry out nucleophilic attack on the scissile phosphate. This was investigated by site-directed mutagenesis of Gly-194 and analysis of the purified mutant enzymes. The results demonstrate that the conformational flexibility around Gly-194 is critical for DNA cleavage to take place to lead to subsequent relaxation of negatively supercoiled DNA.

View larger version (46K): [in this window] [in a new window]   FIG. 1. The proposed hinge region in the three-dimensional structure of the 67-kDa N-terminal domain of E. coli DNA topoisomerase I (Protein Data Bank number 1ECL [PDB] ) (a) and the non-covalent complex of E. coli DNA topoisomerase III with an 8-base single-stranded oligonucleotide (Protein Data Bank number 1I7D [PDB] ) (b). The helix found to be shifted in the E. coli DNA topoisomerase III complex (10) is shown in the ribbon form. Arg-197 and Gln-199 in this helix interact with the phosphodiester backbone.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Relaxation Activity Assay—Wild-type and mutant topoisomerases were diluted serially for a relaxation activity assay in a reaction volume of 20 µl with 10 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.1 mg/ml gelatin, 6 mM MgCl₂, and 0.5 µg of CsCl gradient-purified supercoiled plasmid DNA. For the relaxation assay of activity in total E. coli soluble lysates, 10 µg of yeast tRNA was also included to inhibit the nuclease activities in the lysates. The reaction was terminated after a 30-min incubation at 37 °C by the addition of 5 µl of 50 mM EDTA, 50% glycerol, and 0.5% (v/v) bromphenol blue. The DNA was analyzed by electrophoresis in a 0.8% (w/v) agarose gel with TAE buffer (40 mM Tris acetate, pH 8.1, 2 mM EDTA). After staining with ethidium bromide, the gel was photographed over UV light.

Cleavage of Single-stranded DNA—To prepare for a cleavage substrate with a strong topoisomerase I cleavage site (14, 15), PCR was carried out with the forward primer (5'-CTCTGGCGGTGATAATGGTT-3') and reverse primer (5'-CTCTTCCGCATAAACGCTT-3') using phage DNA as the template and Taq DNA polymerase. The forward primer was labeled prior to the PCR reaction with T4 polynucleotide kinase and [-³²P]ATP so that the top strand of the 203-bp PCR product was labeled at the 5'-end. After electrophoresis in a 1.4% agarose gel, the PCR product was gel-purified using the GenElute spin column (Sigma). The eluted DNA was ethanol-precipitated and resuspended in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Prior to the reaction with topoisomerase, the DNA was denatured to single strands by heating at 95 °C for 5 min. After incubation with the wild-type or mutant topoisomerase at 37 °C for 30 min, trapping of the covalent enzyme-DNA complex and cleaved DNA was achieved by the addition of 0.1 M NaOH. After neutralization (14), the DNA was electrophoresed in an 8% sequencing gel followed by autoradiography of the dried gel to visualize the 5'-end-labeled DNA cleavage products.

Gel Electrophoretic Mobility Shift Assay—A 39-base oligonucleotide 5'-GTTATGCAATGCGCTTTGGGCAAACCAAGAGAGCATAAC-3' (PAGE purified from Sigma-Genosys) with a strong cleavage site for E. coli DNA topoisomerase I (14, 15) was labeled at the 5'-end with T4 polynucleotide kinase and [-³²P]ATP. Reaction mixtures (10 µl) containing 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml bovine serum albumin, 12% glycerol, 1 pmol of the labeled 39-mer, and 0.5–4 pmol (48–388 ng) of wild-type or mutant topoisomerase I were incubated at 37 °C for 5 min. The formation of the gel electrophoretic mobility shift complex was measured by electrophoresis in a 6% polyacrylamide gel with an electrophoresis buffer of 45 mM Tris borate, pH 8.3, 1 mM EDTA as described (16) and quantitated with the PhosphorImager Storm 760.

Oligonucleotide Cleavage Assay—Wild-type and mutant enzymes were incubated with the 5'-end-labeled oligonucleotide used in the gel shift assay (5 pmol in 30 µl of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA) at 37 °C. At different time points, 3-µl aliquots of the reaction mixture were removed and mixed with an equal volume of stop solution (79% formamide, 0.2 M NaOH, 0.04% bromphenol blue). The samples were heated at 80 °C for 5 min before electrophoresis in a 15% sequencing gel. The fraction of oligonucleotide cleaved by the enzyme (following CAAT in the oligonucleotide sequence) was determined by quantitative analysis with the PhosphorImager Storm 760.

Nuclease P1 and DNase I Digestion—Conditions were modified from those described previously (17). For nuclease P1 digestion, the 5'-end-labeled 39-base oligonucleotide (0.2 pmol) was incubated with increasing amounts of wild-type and mutant topoisomerase I in 5 µl of 40 mM Tris-HCl, pH 8.0, 0.1 mg/ml bovine serum albumen, 1 mM magnesium chloride for 4 min at 37 °C. Nuclease P1 (100 ng from Roche Applied Science) was added, and incubation was continued for 2 min before the addition of EDTA to 10 mM and an equal volume of 90% formamide, 0.05% bromphenol blue. After heating at 95 °C for 5 min, the reaction products were electrophoresed in a 15% sequencing gel and visualized with the PhosphorImager Storm 760. The same procedures were used for DNase I digestion except that 0.2 pmol of a 59-base-long oligonucleotide with the same preferred topoisomerase I cleavage site near the junction of the single- and self-complementary double-stranded regions (5'-GCCCTGAAAGATTATGCAATGCGCTTTGGGCAAACCAAGAGAGCATAATCTTTCAGGGC-3') labeled at the 5'-end was used as the substrate with 50 ng of DNase I added to each reaction.

Limited Proteolytic Digestion—Limited digestion of wild-type and mutant topoisomerase I enzymes was carried out with 5 µg of protein in 20 mM potassium phosphate, pH 7.5, 20 mM KCl, 0.2 mM dithiothreitol using sequencing grade Glu-C endoproteinases (Roche Applied Science) at 1:100 w/w ratio at 37 °C for the indicated lengths of time. The digestion was stopped by the addition of an equal volume of 2x gel loading buffer for SDS gel and immersion in boiling water for 5 min. The digestion mixtures were analyzed by electrophoresis in a 15% SDS-polyacrylamide gel followed by staining with Coomassie Blue.

Intrinsic Tryptophan Fluorescence Measurements—Fluorescence measurements were performed with the CARY Eclipse fluorescence spectrophotometer with excitation and 295 nm at room temperature (25 °C). The spectral bandwidths were 5 and 10 nm, respectively, for excitation and emission. The wild-type and mutant topoisomerase I were present at 0.1 mg/ml in 20 mM potassium phosphate, pH 7.4, 20 mM KCl.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
Substitution at Gly-194 in the Potential Hinge Region Resulted in Loss of Relaxation and DNA Cleavage Activities— Random substitutions were generated for Gly-194 by oligonucleotide-directed in vitro mutagenesis, and six different substitution mutants for Gly-194 were identified by DNA sequencing of the individual plasmid pRV10 DNA isolates (Fig. 2). Western blot analysis of the expression level of the mutated topoisomerase in the total protein lysates of E. coli GP200 (topA) cells expressing these mutant topoisomerases showed that all six mutant topoisomerases were accumulated in the soluble extracts at levels comparable with wild-type topoisomerase I (Fig. 2a), so the mutations at Gly-194 did not appear to have resulted in insoluble or unstable topoisomerase I proteins. When assayed with negatively supercoiled plasmid DNA, no relaxation activity was detectable in the total lysates of E. coli GP200 (topA) cells expressing these six mutant topoisomerases (Fig. 2b). Relaxation activity was readily detectable for the GP200 lysate expressing the wild-type topoisomerase I even after a 10-fold dilution of the lysate. The G194R and G194A mutant enzymes expressed in GP200 were purified to >95% homogeneity with procedures similar to those used for the wild-type topoisomerase I and assayed for relaxation activity (Fig. 3). The G194R mutant topoisomerase I had no detectable relaxation activity, whereas the G194A mutant enzyme had an 40-fold reduction in activity when compared with the wild-type topoisomerase I.

View larger version (55K): [in this window] [in a new window]   FIG. 2. Comparison of the expression level of the six Gly-194 substitution topoisomerase I mutants and relaxation activity detected in the total soluble lysates of E. coli GP200 expressing these substitution mutants. a, Western blot analysis of expression level of wild-type (Wt) and mutant topoisomerases with Gly-194 converted to the amino acid indicated. b, relaxation of negatively supercoiled plasmid DNA by the total soluble lysates. C, no lysate added; dash, E. coli GP200 lysate; dWt, wild-type lysate diluted 1:10.

View larger version (43K): [in this window] [in a new window]   FIG. 3. Relaxation activity of purified G194R and G194A mutant topoisomerases. Plasmid pRV10 DNA was incubated with serially diluted wild-type or mutant enzyme. C, control, no enzyme added; S, negatively supercoiled circular DNA; N, nicked/relaxed circular DNA.

View larger version (54K): [in this window] [in a new window]   FIG. 4. Cleavage of 5'-end-labeled single-stranded DNA by wild-type and mutant topoisomerase I. C, control, no enzyme added.

View larger version (24K): [in this window] [in a new window]   FIG. 5. Cleavage of single-stranded 39-base oligonucleotide by wild-type and G194A mutant topoisomerase I. The 5'-end-labeled single-stranded oligonucleotide with a strong topoisomerase I cleavage site (5 pmol) was incubated with different amounts of wild-type or G194A mutant enzyme, and the time course of cleavage was followed by gel electrophoresis and PhosphorImager analysis.

View larger version (19K): [in this window] [in a new window]   FIG. 6. Binding of wild-type and G194R and G194A mutant topoisomerases to a 39-base single-stranded oligonucleotide determined by gel electrophoretic mobility shift assay. The results plotted represent the average from three sets of experimental data. Up to 4 pmol of enzyme was used to limit the degree of cleavage products being generated by the wild-type topoisomerase I.

View larger version (96K): [in this window] [in a new window]   FIG. 7. Comparison of the nuclease protection patterns generated by wild-type and mutant topoisomerases. Nuclease P1 digestions were carried out in the presence of wild-type or G194R and G194A mutant enzymes as described under "Experimental Procedures." The major cleavage product from wild-type topoisomerase I is indicated by the arrow. The nuclease P1 digestion product, marked with an asterisk, was enhanced in the presence of the mutant topoisomerases but not by the binding of wild-type topoisomerase I.

View larger version (93K): [in this window] [in a new window]   FIG. 8. Comparison of the DNase I protection patterns generated by wild-type and mutant topoisomerases. DNase I digestions on a 59-base 5'-end-labeled oligonucleotide with single- and self-complementary double-stranded regions was carried out in the presence of either wild-type or G194R and G194A mutant enzymes as described under "Experimental Procedures." The position of the major cleavage product from wild-type topoisomerase I is indicated by the arrow.

View larger version (104K): [in this window] [in a new window]   FIG. 9. Limited Glu-C proteolysis of wild-type and G194A and G194R mutant topoisomerases. Gel electrophoresis in a 15% SDS-polyacrylamide gel and Coomassie blue staining were used to analyze the Glu-C digestion patterns after 5 and 10 min of incubation.

View larger version (32K): [in this window] [in a new window]   FIG. 10. Intrinsic fluorescence of wild-type and G194R and G194A mutant topoisomerases. a, comparison of intrinsic fluorescence of wild-type and G194R, G194A, and Y319F mutant topoisomerases. b–e, effect of the addition of negatively supercoiled plasmid pBR322 DNA on the intrinsic fluorescence of wild-type and G194R, G194A, and Y319F mutant topoisomerases. f, plot of the change in fluorescence emission at 340 nm with the amount of DNA added.

To demonstrate that the change in fluorescence observed for the wild-type topoisomerase I precedes the DNA cleavage step, the topoisomerase I mutant with the active-site tyrosine mutated to phenylalanine was also analyzed. Its intrinsic fluorescence was found to be similar to that of the wild-type enzyme (Fig. 10a). Upon the addition of pBR322 DNA, there was also an increase in intrinsic fluorescence emission at about half of the magnitude observed for the wild-type enzyme (Fig. 10, e and f). Therefore this active site mutant upon binding to supercoiled DNA could also undergo a conformational change leading to the increase in fluorescence even though it cannot form the covalent complex with DNA. In contrast, the G194R and G194A mutants were found to be defective for this conformational change.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
A number of conserved amino acids around the active site of E. coli DNA topoisomerase I have been shown by site-directed mutagenesis studies (18–20) to be involved in the DNA cleavage step of catalysis by the enzyme. The proposed roles for these conserved amino acids during DNA cleavage include binding of the scissile phosphate, stabilization of the transition state, and leaving group during nucleophilic attack by the active-site tyrosine. These are directly related to the chemical functions present on the side chains of these residues. However, other amino acids in the enzyme at a greater distance from the active-site tyrosine are likely to be required for the conformational changes involved in catalysis instead of direct chemical involvement in the DNA cleavage and religation. These residues that are important for enzyme conformational changes instead of the chemical steps in the enzyme mechanism remain to be identified.

Based on the crystal structure of E. coli topoisomerase III complexed to single-stranded oligonucleotide, the movement of an helix was proposed to be important for the positioning of the scissile phosphate at the active site of type IA DNA topoisomerases (10). It is hypothesized here that the flexibility of the conserved Gly-194 residue at the N terminus of this helix in E. coli topoisomerase I is important for its function as a hinge for this movement. To assess the importance of the hypothesized hinge function, the Gly-194 of E. coli topoisomerase I was mutated to six other amino acids. All six substitutions were found to result in a significant loss of enzyme relaxation activity. Detailed analysis of the purified G194A and G194R mutants showed that the reduction of relaxation activity was 40-fold for the alanine-substituted mutant, whereas no relaxation activity was retained for the more bulky arginine substitution. The loss of relaxation activity resulted primarily from the >100-fold reduction in DNA cleavage activity. The decrease in overall non-covalent complex formation due to the mutations was relatively minor as the mutant enzymes could still form a gel mobility shift complex with the oligonucleotide substrate and protect the bound DNA from P1 nuclease and DNase I digestions. There was some difference in protein folding between the wild-type enzyme and the G194R mutant with the glycine substituted by the more bulky arginine, but the more conservative G194A mutation appeared to have little effect on the enzyme structure in the absence of DNA, as assessed by limited proteolysis and intrinsic fluorescence measurement. A comparison of the response of the intrinsic fluorescence upon the addition of negatively supercoiled DNA showed that the wild-type topoisomerase underwent a conformational change affecting the environment of the tryptophan residues. A similar increase in intrinsic protein fluorescence was also observed for the active-site Y319F mutant topoisomerase I demonstrating that the conformational change leading to the fluorescence increase preceded the formation of the cleaved covalent complex. This increase in intrinsic fluorescence was not detected with the Gly-194 mutant topoisomerases, in agreement with the Gly-194 residue being responsible for a conformational change that takes place after an initial non-covalent binding to DNA and before DNA cleavage takes place. Therefore, in the non-covalent complex formed by the G194R and G194A mutants, the DNA substrate was likely not positioned correctly for nucleophilic attack to take place on the scissile phosphate by the active-site tyrosine.

The sensitivity of enzyme cleavage and relaxation activity to substitution at Gly-194 supports the importance of this residue in the overall topoisomerase I mechanism. There are many known examples of critical glycine residues acting as hinges in the enzyme conformational change required for catalysis (21–24). For example, a glycine to arginine mutation in the hinge region of E. coli tryptophan synthase also blocked the conformational switching (21). A glycine to tryptophan mutation was used to demonstrate the role of the glycine in the conformational change of human O-6-alkylguanine DNA-alkyltransferase upon DNA binding (22). Even though the Gly-194 of E. coli DNA topoisomerase I is located at a considerable distance from the active-site tyrosine, its substitution by another amino acid could still affect the DNA cleavage step drastically. It is likely to be required for shifting the helix containing Arg-195 and Gln-197 in place to interact with the DNA substrate specifically so that the scissile phosphate could be properly aligned for nucleophilic attack by Tyr-319. The Gly-132 of vaccinia DNA topoisomerase I located at an interdomain hinge is invariant among type IB DNA topoisomerases (25). Mutagenesis results obtained previously (25) suggested that this glycine residue in vaccinia DNA topoisomerase I is required for a precleavage activation step. This is similar to the role proposed for the Gly-194 of E. coli DNA topoisomerase I in this study.

This conformational change involving Gly-194 as the hinge is only one of the conformational changes likely to be required for a complete cycle of linking number change catalyzed by E. coli DNA topoisomerase I. After DNA cleavage takes place, the spacing between the 5'-phosphate and 3'-hydroxyl groups of the cleaved DNA must be increased to allow DNA strand passage, and then the spacing had to be decreased again to allow DNA religation to take place. The mechanisms of these conformational changes remain to be elucidated.

	FOOTNOTES

 
^* This work was supported by Grant GM54226 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 914-594-4061; Fax: 914-594-4058; E-mail: yuk-ching_tse-dinh{at}nymc.edu.

¹ S. Gadgil and Y.-C. Tse-Dinh, unpublished results.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 

1. Champoux, J. J. (2001) Annu. Rev. Biochem. 70, 369–413[CrossRef][Medline] [Order article via Infotrieve]
2. Wang, J. C. (1996) Annu. Rev. Biochem. 65, 635–692[CrossRef][Medline] [Order article via Infotrieve]
3. Wang, J. C. (2002) Nat. Rev. Mol. Cell Biol. 3, 430–440[CrossRef][Medline] [Order article via Infotrieve]
4. Tse-Dinh, Y.-C. (1998) Biochim. Biophys. Acta 1400, 19–27[Medline] [Order article via Infotrieve]
5. Keck, J. L., and Berger, J. M. (1999) Nat. Struct. Biol. 6, 900–902[CrossRef][Medline] [Order article via Infotrieve]
6. Champoux, J. J. (2002) Trends Pharmacol. Sci. 23, 199–201[CrossRef][Medline] [Order article via Infotrieve]
7. Lima, C. D., Wang, J. C., and Mondragón, A. (1994) Nature 367, 138–145[CrossRef][Medline] [Order article via Infotrieve]
8. Mondragón, A., and DiGate, R. (1999) Structure Fold. Des. 7, 1373–1383[Medline] [Order article via Infotrieve]
9. Rodriguez, A. C., and Stock, D. (2002) EMBO J. 21, 418–426[CrossRef][Medline] [Order article via Infotrieve]
10. Changela, A., DiGate, R. J., and Mondragón, A. (2001) Nature 411, 1077–1081[CrossRef][Medline] [Order article via Infotrieve]
11. Caron, P. R. (1999) in DNA Topoisomerase Protocol: DNA Topology and Enzymes (Bjornsti, M.-A., and Osheroff, N., eds) Vol. 1, pp. 279–316, Humana Press, Totowa, NJ
12. Zhu, C.-X., and Tse-Dinh, Y.-C. (1999) in DNA Topoisomerase Protocols: DNA Topology and Enzymes (Bjornsti, M.-A., and Osheroff, N., eds) Vol. 1, pp. 145–151, Humana Press, Totowa, NJ
13. Wang, J. C., Peck, L. J., and Becherer, K. (1982) Cold Spring Harbor Symp. Quant. Biol. 47, 85–91
14. Tse, Y.-C., Kirkegaard, K., and Wang, J. C. (1980) J. Biol. Chem. 255, 5560–5565[Free Full Text]
15. Kirkegaard, K., Pflugfelder, G., and Wang, J. C. (1984) Cold Spring Harbor Symp. Quant. Biol. 49, 411–419[Medline] [Order article via Infotrieve]
16. Ahumada, A., and Tse-Dinh, Y.-C. (2002) BMC Biochemistry 3, 13[Medline] [Order article via Infotrieve]
17. Zhang, H. L., Malpure, S., and DiGate, R. J. (1995) J. Biol. Chem. 270, 23700–23705[Abstract/Free Full Text]
18. Zhu, C.-X., Roche, C. J., Papanicolaou, N., Dipietrantonio, A., and Tse-Dinh, Y.-C. (1998) J. Biol. Chem. 273, 8783–8789[Abstract/Free Full Text]
19. Chen, S.-J., and Wang, J. C. (1998) J. Biol. Chem. 273, 6050–6056[Abstract/Free Full Text]
20. Perry, K., and Mondragón, A. (2002) J. Biol. Chem. 277, 13237–13245[Abstract/Free Full Text]
21. Zhao, G.-P., and Somerville, R. L. (1993) J. Biol. Chem. 268, 14921–14931[Abstract/Free Full Text]
22. Rafferty, J. A., Wibley, J. E., Speers, P., Hickson, I., Margison, G. P., Moody, P. C., and Douglas, K. T. (1997) Biochim. Biophys. Acta 1342, 90–102[CrossRef][Medline] [Order article via Infotrieve]
23. Grant, G. A., Hu, Z., and Xu, X. L. (2001) J. Biol. Chem. 276, 17844–17850[Abstract/Free Full Text]
24. McCormack, E. A., Llorca, O., Carrascosa, J. L., Valpuesta, J. M., and Willison, K. R. (2001) J. Struct. Biol. 135, 198–204[CrossRef][Medline] [Order article via Infotrieve]
25. Wittschieben, J., and Shuman, S. (1997) Nucleic Acids Res. 25, 3001–3008[Abstract/Free Full Text]

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				B. Cheng, J. Feng, V. Mulay, S. Gadgil, and Y.-C. Tse-Dinh Site-directed Mutagenesis of Residues Involved in G Strand DNA Binding by Escherichia coli DNA Topoisomerase I J. Biol. Chem., September 17, 2004; 279(38): 39207 - 39213. [Abstract] [Full Text] [PDF]

				E. Moreno, C. Tovar-Palacio, P. de los Heros, B. Guzman, N. A. Bobadilla, N. Vazquez, D. Riccardi, E. Poch, and G. Gamba A Single Nucleotide Polymorphism Alters the Activity of the Renal Na+:Cl- Cotransporter and Reveals a Role for Transmembrane Segment 4 in Chloride and Thiazide Affinity J. Biol. Chem., April 16, 2004; 279(16): 16553 - 16560. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 279/10/8648    most recent M312095200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cheng, B. Articles by Tse-Dinh, Y.-C. Search for Related Content PubMed PubMed Citation Articles by Cheng, B. Articles by Tse-Dinh, Y.-C. Social Bookmarking                      What's this?