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J. Biol. Chem., Vol. 279, Issue 12, 10892-10900, March 19, 2004
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From the Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021
Received for publication, November 14, 2003 , and in revised form, December 28, 2003.
| ABSTRACT |
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| INTRODUCTION |
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The enzyme Fcp1 is the major protein serine phosphatase responsible for removing phosphates from the CTD (2, 816). Fcp1 orthologs are present in all known eukaryal proteomes, and the enzyme is essential for cell viability in budding and fission yeast (10, 15). A partial deficiency of human Fcp1 is associated with an autosomal recessive developmental disorder characterized by cataracts, facial dysmorphism, and peripheral neuropathy (17).
Fcp1 catalyzes the metal-dependent hydrolysis of phosphoserine from the CTD in the context of the pol II elongation complex, isolated pol II, and (in the case of Schizosaccharomyces pombe Fcp1) synthetic CTD phosphopeptide substrates. Fcp1 also hydrolyzes the nonspecific substrate p-nitrophenyl phosphate (13, 16). Fcp1 orthologs are relatively large polypeptides (723 aa in S. pombe; 732 aa in Saccharomyces cerevisiae; 961 aa in humans) consisting of a conserved central catalytic domain flanked by variable N- and C-terminal segments that are dispensable for CTD phosphatase activity in vitro (16, 18), but are collectively required for Fcp1 function in vivo (19). The minimal phosphatase domain of S. pombe Fcp1 spans amino acids 156580 (18). The catalytic domain is itself composed of two domain modules: an N-terminal FCPH (FCP1 homology) domain spanning S. pombe Fcp1 residues 140326 (see Fig. 1) and a C-terminal BRCT (BRCA1 C terminus) domain, both of which are essential for Fcp1 phosphatase activity in vivo and in vitro (14, 16, 19). Although the catalytic contributions made by the BRCT domain are not known, a recent report implicates the BRCT domain in the initial binding of Fcp1 to the phosphorylated CTD (20). A wealth of data implicate the FCPH domain as the seat of the phosphatase active site.
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We previously employed site-directed mutagenesis to locate candidate catalytic residues of S. pombe Fcp1 (16, 18). We found that alanine substitutions for Asp170, Asp172, Arg223, Asp258, Lys280, Asp297, and Asp298 abolished phosphatase activity with either p-nitrophenyl phosphate or CTD-PO4 as substrates. Structure-activity relationships were subsequently determined by introducing conservative substitutions at each essential position. Because the seven essential amino acids identified in S. pombe Fcp1 are conserved in Fcp1 orthologs from other organisms, we predicted that they comprise the phosphatase active site.
A goal of the present study was to more fully delineate the phosphatase active site and the catalytic mechanism of Fcp1. Therefore, we conducted additional mutational analysis of the FCPH domain, guided by sequence comparisons to diverse Fcp1 homologs (Fig. 1). We report the effects of 17 mutations at 11 conserved positions of S. pombe Fcp1, which identify four new residues (Thr174, Tyr237, Thr243, and Tyr249) as important for activity with both specific and nonspecific substrates. Based on the crystal structures available for related DXDX(T/V) phosphatases, we discuss plausible catalytic roles for the essential side chains of Fcp1.
Although the mutational studies illuminate the chemical mechanism of Fcp1, they do not instruct us on the basis for specific recognition of the phosphorylated CTD. Previously, we showed that S. pombe Fcp1 displays an inherent preference for a particular CTD phosphorylation array. Using equivalent 28-aa CTD peptides of identical amino acid sequence and phosphoserine content, which differed only in the positions of phosphoserine within the tetraheptad sequence, we found that Fcp1 was 10-fold more active in dephosphorylating Ser2-PO4 than Ser5-PO4 (16). Here we systematically investigated the structural features of the CTD Ser2-PO4 substrate that promote optimal phosphatase activity. Our results show that: (i) Fcp1 acts distributively during the hydrolysis of substrates containing tandem Ser2-PO4 heptads; (ii) Fcp1 fails to hydrolyze the N-terminal Ser2-PO4-containing heptad when the substrate is phased as (Y1S2P3T4S5P6S7)n; (iii) the minimal optimal CTD substrate for Fcp1 is a single heptad of phasing S5P6S7Y1S2P3T4; and (iv) single alanine mutations of flanking residues Tyr1 or Pro3 result in 6-fold decrements in CTD phosphatase activity.
| EXPERIMENTAL PROCEDURES |
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CTD PhosphopeptidesThe following CTD Ser2-PO4 peptides containing an unmodified N-terminal amine and a C-terminal acid were synthesized and purified by the Sloan-Kettering Microchemistry Core Laboratory as described in Ref. 29 (the Ser2-PO4 residues are underlined): 28-mer YSPTSPSYSPTSPSYSPTSPSYSPTSPS; 14-mer YSPTSPSYSPTSPS; 14-mer YSPTSPSYSPTSPS; 14-mer YSPTSPSYSPTSPS; 12-mer PTSPSYSPTSPS; 10-mer SPSYSPTSPS; 8-mer SYSPTSPS; 10-mer (Y1A), SPSASPTSPS; 10-mer (P3A), SPSYSATSPS. Additional CTD Ser2-PO4 peptides containing an N-terminal amine and a C-terminal amide were as follows: 9-mer SPSYSPTSP; 8-mer SPSYSPTS; 7-mer SPSYSPT. The peptides were dissolved in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA and stored at 4 °C. The molar concentrations of the phosphopeptides were initially estimated from the absorbance at 274 nM using an extinction coefficient of 1.4 x 103 M1 for tyrosine. The content of Ser2-PO4 was then determined for each peptide by measuring the release of inorganic phosphate after digestion with calf intestinal phosphatase (CIP; purchased from Roche Applied Science) as follows: CIP reaction mixtures (25 µl) containing
2.5 nmol of CTD phosphopeptide and CIP (2 units) were incubated for 60 min at 37 °C. The reactions were quenched by adding 0.5 ml of malachite green reagent (BIOMOL Research Laboratories, Plymouth Meeting, PA). Release of phosphate was determined by measuring A620 and interpolating the value to a phosphate standard curve. The phosphopeptide concentrations measured by CIP were in good agreement (±10%) with the concentrations calculated from the UV absorbance. The amounts of input CTD Ser2-PO4 substrate specified in the Fcp1 reactions are based on the concentrations determined by CIP digestion.
Phosphatase AssayReaction mixtures (100 µl) containing 50 mM Tris acetate (pH 5.5), 10 mM MgCl2, 10 mM p-nitrophenyl phosphate (pNØP), and Fcp1 as specified were incubated for 30 min at 37 °C. The reactions were quenched by adding 900 µl of 1 M sodium carbonate. Release of p-nitrophenol (pNØ) was determined by measuring A410 and interpolating the value to a pNØ standard curve.
CTD Phosphatase AssayReaction mixtures (25 µl) containing 50 mM Tris acetate (pH 5.5), 10 mM MgCl2, CTD phosphopeptide, and Fcp1 were incubated for 60 min at 37 °C. The reactions were quenched by adding 0.5 ml of malachite green reagent. Release of phosphate was determined by measuring A620 and interpolating the value to a phosphate standard curve.
Product Analysis by MALDI-TOF Mass SpectrometryReaction mixtures (300 µl) containing 50 mM Tris acetate (pH 5.5), 10 mM MgCl2, 25 µM phosphopeptide (YSPTSPS)4 (= 100 µM Ser2-PO4) and 0.3 µM Fcp1 were incubated at 37 °C. Aliquots (25 µl) were withdrawn at the times specified, and the reaction was quenched by adding 10 µl of 5 M formic acid. Aliquots (1 µl) of the mixture were diluted 100-fold with 0.1% formic acid and loaded onto a 2-µl column of Poros 50 R2 reverse-phase chromatography beads (PerSeptive Biosystems, Framingham, MA) packed into an Eppendorf gel-loading tip. The adsorbed peptides were eluted with 8 µl of 30% acetonitrile, 0.1% formic acid. An aliquot (0.5 µl) of the eluted peptide pool was analyzed by matrix-assisted laser-desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS), using a Bruker UltraFlex TOF/TOF instrument (Bruker Daltonics, Bremen, Germany), as described previously (40). The spectra were obtained in the negative ion mode, by averaging multiple acquisitions. The distribution of tetra-, tri-, di-, and monophosphorylated CTD peptides in each sample (Fig. 3B) was calculated as the ratio of the observed peak heights of the individual tetra-, tri-, di-, or monophosphorylated peptides to the sum of the heights of the tetra-, tri-, di-, and monophosphorylated peptide peaks.
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| RESULTS |
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To explore this issue, aliquots of the reaction mixture were withdrawn at serial times, quenched with acid, and analyzed by MALDI-TOF mass spectrometry, which fractionates the peptide pool based on molecular mass. This allowed us to gauge the distribution of products as the input tetraphosphorylated CTD substrate was converted to species of lower mass differing by multiples of 80-Da increments (signifying the loss of integral numbers of phosphate groups). The first instructive findings emerged from the product distribution at the 60-min reaction end point, showing that there was no residual tetraphosphorylated CTD substrate and that 87% of the product corresponded to singly phosphorylated CTD peptide (1xPO4) and 13% corresponded to doubly phosphorylated CTD (2xPO4) (Fig. 3B). These results confirmed that all of the input CTD substrate was reactive with Fcp1 and that the reaction ceased after three of the four phosphates were hydrolyzed. The structural basis for this restricted end point is explored in depth below.
The evolution of the product distribution during the 60-min reaction (Fig. 3B) allowed a clear discrimination between processive and distributive modes of action by S. pombe Fcp1 on a multiply phosphorylated CTD array. In a purely processive mechanism under conditions of substrate excess, Fcp1 would sequentially hydrolyze all available Ser2-PO4 on the peptide to which it initially bound before dissociating to a new substrate peptide. In that event, we would expect to detect at all times a product distribution consisting of a mixture of the unreacted 4xPO4 substrate and the 1xPO4 CTD "end-product," with a steady increase in the abundance of end-product as a function of time and little accumulation of CTD peptides with intermediate phosphorylation states. In contrast, in a purely distributive mechanism, Fcp1 would dissociate from the CTD peptide after hydrolysis of only one Ser2-PO4 position and then be available to rebind to other CTD peptides in the substrate pool. In a distributive mechanism, we would expect to see a serial flux of product through each of the dephosphorylated species, i.e. that the triphosphorylated CTD (3xPO4) predominates at the earliest times before it decays to a diphosphorylated CTD (2xPO4), which is converted in turn to the 1xPO4 CTD end-product. Indeed, the distributive pattern is exactly what we observed (Fig. 3B). At the earliest time analyzed (2 min), virtually all of the input substrate had been consumed and converted primarily to 3xPO4 CTD (78%) and to a lesser extent to 2xPO4 CTD (22%). The 3xPO4 species declined steadily in abundance over 20 min. The 2xPO4 CTD accumulated steadily between 2 and 10 min, plateaued at 1020 min (comprising 62% of the CTD pool) and declined thereafter, concomitant with the steady accumulation of the 1xPO4 product. Note that no 1xPO4 product was detected at the earliest times (
5 min) and that this species did not appear until such time as a significant fraction of the CTD had been converted to 2xPO4 CTD (Fig. 3B). These results provide strong evidence for a distributive catalytic mechanism for hydrolysis of Ser2-PO4. The kinetic profile also hinted that removal of the first phosphate was faster than removal of the second phosphate, which was in turn faster than removal of the third phosphate.
Fcp1 Dephosphorylates a CTD Phosphopeptide Containing Two Heptad RepeatsThe preceding experiment using a tetraheptad CTD Ser2-PO4 substrate showed clearly that Fcp1 is capable of dephosphorylating three of the four heptads. We considered two possible explanations for this result: (i) Fcp1 requires two Ser2-PO4 moieties to bind and hydrolyze the CTD or (ii) Fcp1 requires a minimal length CTD peptide flanking each Ser2-PO4 and that either the N-terminal heptad or the C-terminal heptad of the 28-aa substrate lacks the required flanking residues.
To address this issue, we synthesized a 14-aa diheptad CTD peptide YSPTSPSYSPTSPS phosphorylated at Ser2 of each heptad. Reaction of Fcp1 with 100 µM of the 2xPO4 CTD peptide resulted in Pi release proportional to the amount of input enzyme in the range of 0.161.25 µg of Fcp1 (Fig. 4A, titration curve (
)); 48% of the input phosphoserine was hydrolyzed at saturating enzyme levels (2.55 µg of Fcp1). Apparently, only one of the two available Ser2-PO4 heptads served as a substrate for Fcp1. To determine which phosphoserine was dephosphorylated and which was resistant, we synthesized two 14-mer monophosphorylated CTD peptides, which contained Ser2-PO4 in either the N-terminal heptad (YSPTSPSYSPTSPS) or the C-terminal heptad (YSPTSPSYSPTSPS). Reaction of Fcp1 with 100 µM of the 1xPO4 C-terminal peptide resulted in quantitative Pi release that was proportional to input Fcp1 (Fig. 4A, titration curve (
)). In contrast, Fcp1 was unreactive with 100 µM of the 1xPO4 N-terminal peptide at up to 5 µg of input enzyme (Fig. 4A, titration curve (
)). From the slopes of the titration curves, we calculated that Fcp1 specific activity with the 1xPO4 N-terminal peptide was 0.5% of the specific activity with the 1xPO4 C-terminal peptide substrate. A kinetic analysis under conditions of substrate excess showed that the rate and extent of Pi release were similar for the 14-mer 2xPO4 substrate and the 1xPO4 C-terminal phosphoheptad substrate (Fig. 4B). From the slopes of the curves, we calculated turnover numbers of
0.09 s1 for the two diheptad CTD substrates.
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Defining the Minimal CTD Substrate for S. pombe Fcp1 The requirements for CTD primary structure on the N-terminal side of the Ser2-PO4 residue were probed by comparing Fcp1 activity with a series of N-terminal deleted, singly phosphorylated 12-mer (PTSPSYSPTSPS), 10-mer SPSYSPTSPS, and 8-mer (SYSPTSPS) peptides, which contained five amino acids C-terminal to Ser2-PO4 and either 6, 4, or 2 amino acids on the N-terminal side, respectively (Fig. 5A). Fcp1 catalyzed quantitative release of Pi from the 12-mer PTSPSYSPTSPS and 10-mer SPSYSPTSPS peptides with similar specific activities (these being 80 and 94%, respectively, of the specific activity with the 14-mer diheptad 1xPO4 CTD substrate used in Fig. 4). In contrast, Fcp1 was much less active in dephosphorylating the 8-mer peptide SYSPTSPS (specific activity being 5% of that observed with the diheptad 1xPO4 CTD substrate). We conclude that 4 amino acids N-terminal to the Ser2-PO4 sufficed for optimal Fcp1 activity.
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Role of CTD Residues Tyr1 and Pro3 in Fcp1-catalyzed Dephosphorylation of Ser2To investigate the role of the amino acid side chains flanking phosphoserine as determinants of Fcp1 activity, we tested mutated versions of the 10-mer CTD phosphopeptide SPSYSPTSPS, wherein Tyr1 or Pro3 was replaced individually by alanine (Fig. 6). The titration profiles of the reaction of Fcp1 with 100 µM of the Y1A (SPSASPTSPS) or P3A (SPSYSATSPS) substrates displayed a clear shift to the right compared with the wild-type CTD peptide (Fig. 6). The specific activities of Fcp1 with the Y1A and P3A 10-mer substrates were 16 and 17%, respectively, of the wild-type 10-mer CTD substrate. Thus, Tyr1 and Pro3 are both important for optimal CTD phosphatase activity of Fcp1. As discussed below, these results provide insight to the basis for preferential dephosphorylation of CTD Ser2-PO4 by S. pombe Fcp1.
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in Fig. 1), seven were found to be essential for phosphatase activity (shaded boxes in Fig. 1) (16, 18). Here we extended the analysis by introducing single alanine changes at 11 new amino acids of the FCPH domain that are conserved in other Fcp1 orthologs. The mutated positions are indicated by | in Fig. 1. We focused in particular on basic (Lys163, Arg164, Lys221, and Lys247), acidic (Asp273), and polar (Gln173, Thr174, Tyr237, Tyr249, Thr243, and Ser270) side chains. Wild-type Fcp1 and the 11 Fcp1-Ala mutants were produced in bacteria as His10-tagged fusions and purified from soluble bacterial extracts by nickel-agarose chromatography. SDS-PAGE analysis of the imidazole eluate fractions showed that the preparations were highly enriched with respect to the His-Fcp1 polypeptide (Fig. 7).
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)), which released 17 nmol of pNØ per microgram of protein in 30 min. The specific activity of the T243A protein was <0.5% of the activity of wild-type Fcp1, whereas Y249A, Y237A, and T174A were 2%, 5, and 9% as active as wild-type Fcp1, respectively (Fig. 8A). Our criterion for a significant mutational effect is one that elicits at least a 10-fold decrement in specific activity compared with wild-type Fcp1. Thus, we conclude that the Thr174, Tyr237, Tyr249, and Thr243 side chains are important for Fcp1 activity. The specific activities of the other Fcp1-Ala proteins, expressed as the percent of wild-type, were as follows: K163A (85%) R164A (34%), Q173A (48%), K221A (67%), K247A (140%), S270A (160%), and D273A (90%). We surmise that the Lys163, Arg164, Gln173, Lys221, Lys247, Ser270, and Asp273 side chains do not contribute significantly to catalysis of the generic phosphatase reaction.
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Structure-function Relationships at Essential Residues of S. pombe Fcp1To further evaluate the contributions of Thr174, Tyr237, Thr243, and Tyr249 to the phosphatase reaction, we tested the effects of conservative substitutions. Threonine was replaced by serine and valine; tyrosine was changed to phenylalanine. The recombinant T174S, T174V, Y237F, T243S, T243V, and Y249F proteins were purified from soluble bacterial extracts by nickel-agarose chromatography (Fig. 7B). Hydrolysis of pNØP was measured as a function of enzyme concentration for wild-type Fcp1 and the six conservative mutants (Fig. 9A). Introduction of a serine in lieu of Thr243 restored activity to 48% of the wild-type level, compared with <1% activity for T243A. The valine substitution had no salutary effect (2% of wild-type activity), implying that the hydroxyl group at position 243 is essential for catalysis. Replacement of Thr174 with serine revived activity to 40% of the wild-type level, compared with 9% activity for the T174A mutant, whereas the valine mutant (with 2% of wild-type activity) was even more defective than T174A. Thus, the hydroxyl group of Thr174 is the functionally relevant substituent. Conservative replacement of Tyr237 with Phe resulted in virtually complete gain of function, to 95% of wild-type activity, compared with the Y237A mutant, which was 2% as active as wild-type Fcp1. The hydroxyl group of the aromatic residue at position 237 is apparently not important for Fcp1 function. This is consistent with the fact that a Phe side chain is present at the equivalent position of S. cerevisiae Fcp1 (Fig. 1). Conservative replacement of Tyr249 with Phe elicited a partial gain of function (to 19% of wild-type activity, versus 5% for Y249A), indicating that the hydroxyl moiety of Tyr249, although not strictly essential, enhances Fcp1 phosphatase activity by a factor of 5. The Tyr249 side chain is strictly conserved in all of the Fcp1 orthologs aligned in Fig. 1.
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| DISCUSSION |
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Our emphasis has been to understand the intrinsic catalytic properties and specificity of Fcp1, which is a necessary step if one is to ultimately evaluate how positive and negative regulatory factors, covalent modifications of Fcp1 (31, 39) or cis-trans isomerization of the CTD prolines (46), impact on Fcp1 function. We have developed S. pombe Fcp1 as a model system in view of the relative ease of obtaining active recombinant protein and the fact that S. pombe Fcp1 displays a comparatively vigorous intrinsic phosphatase activity with either pNØP or synthetic CTD phosphopeptide substrates. We showed previously that recombinant S. pombe Fcp1 was 10-fold more active at its optimal pH of 5.5 in dephosphorylating a 28-mer CTD peptide containing Ser2-PO4 than an otherwise identical 28-mer containing Ser5-PO4. Here we confirmed that S. pombe Fcp1 also prefers Ser2-PO4 at pH 7.0. The present study substantially advances our knowledge of Fcp1 mechanism by: (i) demonstrating that S. pombe Fcp1 is intrinsically distributive with respect to the phospho-CTD substrate; (ii) defining the minimal CTD Ser2-PO4 substrate; and (iii) underscoring the role of CTD primary structure as a determinant of Fcp1 activity.
The issue of whether Fcp1 is intrinsically processive or distributive is significant in light of a previous report that human Fcp1 appeared to act processively in dephosphorylating 32P-labeled pol II in the presence of TFIIF (32). S. pombe Fcp1 is clearly distributive with respect to the CTD. Although we cannot rule out a model whereby the fission yeast and human enzyme have fundamentally different mechanisms, we speculate that the apparent processive action of the human enzyme on intact pol II does not arise because the enzyme remains bound to the CTD until the CTD is exhaustively dephosphorylated ("true" processivity), but rather that human Fcp1 is bound elsewhere to the body of pol II (15,30) and that this association allows the polyphosphorylated pol II CTD in cis to reiteratively bind, react, and dissociate at the Fcp1 active site ("apparent" processivity). Were Fcp1 truly processive with respect to the CTD, then one would have to posit the existence of mechanisms to protect the CTD from wholesale dephosphorylation after a single Fcp1 binding event (e.g. via sequestration of some heptads by virtue of binding to other proteins, or via factors that promote Fcp1 dissociation from the CTD).
Knowledge of the atomic structure of the CTD and how such parameters as CTD length, amino acid sequence, and phosphorylation arrays influence CTD-PO4 effector functions has emerged from recent studies of the interaction of the mRNA capping apparatus with the phosphorylated CTD (29, 33, 34). A key inference from these studies is that the CTD-PO4 is conformationally plastic and capable of adopting diverse structures that are templated by the proteins to which the CTD binds. Thus, it is incumbent to delineate for each CTD-interacting protein or enzyme the basis for CTD recognition.
The present analysis of the hydrolysis by S. pombe Fcp1 of synthetic CTD phosphopeptides illuminates the contributions of CTD length, heptad phasing, and individual conserved CTD side chains to CTD phosphatase activity. These insights could only have been obtained using defined substrates. The reports that Fcp1 enzymes from species other than S. pombe appear to catalyze the hydrolysis of phosphoserine from the CTD in the context of the pol II elongation complex or isolated pol II, but not in the context of a recombinant CTD fusion protein (12, 30), have been cited as casting doubt on the value of results obtained for S. pombe Fcp1 with CTD phosphopeptide substrates (42). We would emphasize here that orthologous enzymes from different species cannot be presumed to have the same inherent biochemical properties, or to engage in the same repertoire of protein-protein interactions that impact on their enzymatic activity. Indeed, this point is underscored by the demonstration that the orthologous triphosphatase and guanylyltransferase components of the fungal mRNA capping apparatus interact differently with each other, and with the pol II CTD, in S. pombe versus S. cerevisiae (33, 44, 45). Rather than viewing the clearly demonstrated capacity of S. pombe Fcp1 to act on CTD phosphopeptides in vitro as an aberration, we see S. pombe Fcp1 as being liberated from the dependence on protein-protein contacts (e.g. with the "body" of pol II or with TFIIF) for CTD phosphatase activity seen with human Fcp1. Moreover, the ability to dephosphorylate CTD phosphopeptide substrates is not a property unique to S. pombe Fcp1 among the growing collection of protein phosphatases known or suspected to act on the pol II CTD in vivo. Yeo et al. (5) have identified several small CTD phosphatases (SCPs) in human cells, which belong to the DXDX(T/V) enzyme family that includes Fcp1. They showed that recombinant human SCP1 has optimal phosphatase activity at pH 5.0 (thereby resembling S. pombe Fcp1) and that human SCP1 readily dephosphorylates the same pair of synthetic 28-mer CTD Ser2-PO4 and Ser5-PO4 peptides we use here and in our prior studies of CTD-PO4 effector functions (16, 18, 29, 33). Human SCP1 displayed a modest (2-fold) preference for Ser5-PO4 over Ser2-PO4 (5). Thus, different DX-DX(T/V) phosphatase family members that act on the CTD clearly do have different inherent specificities.
Knowing now that a properly phased single heptad S5P6S7Y1S2P3T4 suffices for efficient S. pombe Fcp1 phosphatase activity in vitro and that the side chains flanking the phosphoserine are important for Fcp1 activity, we are in a position explain why S. pombe Fcp1 preferentially dephosphorylates Ser2-PO4 versus Ser5-PO4. Inspection of the analogously phased Ser5-PO4 peptide Y1S2 P3T4S5P6S7 shows that the C-terminal flanking dipeptide is similar in both cases (either Pro-Thr or Pro-Ser), whereas the N-terminal flanking peptides are divergent (Ser-Pro-Ser-Tyr versus Tyr-Ser-Pro-Thr), particularly with respect to the presence of a tyrosine immediately upstream of phosphoserine in the preferred Ser2-PO4 CTD. Loss of the Tyr1 side chains reduced Fcp1 activity by a factor of 6, an effect that accounts for most of the disparity between the Ser2 and Ser5 phosphatase activities of S. pombe Fcp1. The similar reduction in activity elicited by the P3A mutation of the CTD underscores that Fcp1 is a proline-directed protein serine phosphatase.
The present mutational analysis of Fcp1 identified four new side chains important for both generic and CTD-specific phosphatase activities: Thr174, Tyr237, Thr243, and Tyr249. Together with the seven essential amino acids identified previously (Asp170, Asp172, Arg223, Asp258, Lys280, Asp297, and Asp298), they likely comprise the phosphatase active site. It had been suggested on the basis of primary structure comparisons and comparative mutational analyses that Fcp1 belongs to a subgroup of DXDX(T/V) acyl-phosphatases that includes bacteriophage T4 polynucleotide 3' phosphatase (18, 3538). The signature of this subfamily is the essential 297DD298 dipeptide located at the distal end of the FCPH domain of Fcp1 (Fig. 1). The ten catalytically essential side chains of the T4 phosphatase include six aspartates, two arginines, one lysine, and one serine (3538).2 The amino acid composition of the T4 phosphatase active site is generally similar to the constellation of essential residues of S. pombe Fcp1, which consists, to date, of five aspartates, one arginine, one lysine, two threonines, and two tyrosines.
Crystallographic snapshots have been obtained for Methanococcus jannaschii phosphoserine phosphatase (PSP), Lactococcus lactis phosphoglucomutase (PGM), and T4 polynucleotide 3' phosphatase at various stages of the catalytic cycle (25, 27, 28, 38). These structures suggested plausible catalytic roles for five of the essential side chains of Fcp1 in either nucleophilic attack on Ser-PO4 (Asp170), general acid-base catalysis (Asp172), transition-state stabilization (Lys280), or metal coordination (Asp297 and Asp298). Reference to the available crystal structures of PSP and PGM suggests that the newly identified essential Fcp1 residue Thr243 is the functional equivalent of a conserved serine side chain (Ser99 in PSP and Ser114 in PGM) that interacts via O
with one of the trigonal phosphate oxygens of the pentacovalent transition state. The equivalent Ser211 residue in T4 phosphatase is essential for activity.2 The catalytic serine is located 31 to 47 aa upstream of a conserved lysine that coordinates one of the other trigonal phosphate oxygens of the transition state. Note that Thr243 of Fcp1 is situated 37 aa upstream of its corresponding essential lysine, Lys280. The structure-activity relationships at Fcp1 Thr243 and T4 phosphatase Ser211 are concordant (i.e. either threonine or serine at these positions supported the activity of both enzymes). Thus, the requirement for a hydroxyamino acid at Fcp1 residue 243 is consistent with the hydrogen bond to the phosphate seen in the PSP and PGM crystals.
Thr174 is important for Fcp1 activity and is located within the signature DXDXT phosphatase motif; all Fcp1 orthologs contain threonine at this position (Fig. 1). The corresponding side chain in PSP (Thr15) engages in a hydrogen bond via O
to the carboxylate O
2 of the essential Asp11 side chain. PSP Asp11 O
2 coordinates the essential divalent cation cofactor, whereas O
1 forms the covalent acylphosphate linkage (25). We posit a similar interaction between Fcp1 Thr174 and Asp170 and suggest that this hydrogen bond helps orient the Asp nucleophile for metal coordination and attack on the phosphorylated substrate.
Definitive evaluation of the Fcp1 mechanism and the substrate specificity determinants suggested by our studies of S. pombe Fcp1 will ultimately hinge on crystallization of Fcp1 bound to a defined CTD phosphopeptide. The delineation of the phased heptamer S5P6S7Y1S2P3T4 as the minimal effective CTD substrate should facilitate cocrystallization experiments.
| FOOTNOTES |
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To whom correspondence should be addressed. Tel.: 212-639-7145; Fax: 212-717-3623; E-mail: s-shuman{at}ski.mskcc.org.
1 The abbreviations used are: CTD, C-terminal domain; pol II, polymerase II; aa, amino acid(s); CIP, calf intestinal phosphatase; pNØ, p-nitrophenol; pNØP, p-nitrophenyl phosphate; MALDI-TOF, matrix-assisted laser desorption ionization time-of-flight; MS, mass spectrometry; SCP, small CTD phosphatase; PSP, phosphoserine phosphatase; PGM, phosphoglucomutase. ![]()
2 H. Zhu and S. Shuman, unpublished data. ![]()
| ACKNOWLEDGMENTS |
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| REFERENCES |
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