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J. Biol. Chem., Vol. 279, Issue 21, 22585-22594, May 21, 2004
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From the
Department of Biochemistry, Graduate Program in Genetics and Molecular Biology, **Department of Biochemistry and Division of Cancer Biology, and Department of Radiation Oncology, School of Medicine, Emory University, Atlanta, Georgia, 30322 and the ||Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, Texas 76107
Received for publication, January 15, 2004 , and in revised form, March 10, 2004.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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400 to 1400 Ntg1p-recognized DNA lesions per genome necessary to provoke profound biological changes similar in many respects to the phenotypic properties of cancer cells. The BER/NER-defective cells are genetically unstable, exhibiting mutator and hyper-recombinogenic phenotypes. They also exhibit aberrations in morphology, DNA content, and growth characteristics compared with WT, BER-defective, and NER-defective cells. The BER/NER-defective cells also possess increased levels of intracellular reactive oxygen species, activate the yeast checkpoint response pathway via Rad53p phosphorylation in stationary phase, and show profound changes in transcription patterns, a subset of which can be ascribed to responses resulting from unrepaired DNA damage. By establishing a relationship between specific levels of spontaneous DNA damage and the ensuing deleterious biological consequences, these yeast DNA excision repair-defective strains are an informative model for gauging the progressive biological consequences of spontaneous DNA damage accumulation and may have relevancy for delineating underlying mechanisms in tumorigenesis. |
INTRODUCTION |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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BER involves primarily the repair of small, helix non-distorting base lesions and abasic sites (5). It is believed that repair of oxidative damage is mediated primarily by this pathway (6). In BER, the DNA damage is recognized by a specific DNA glycosylase that catalyzes the release of the damaged base and creates an abasic site. In S. cerevisiae, the abasic site can be processed further by either a hydrolytic AP endonuclease such as Apn1p or N-glycosylase-associated AP lyases such as Ntg1p or Ntg2p. DNA strand cleavage resulting from the actions of these proteins results in modified strand break termini that must be further processed to become substrates for subsequent repair proteins such as DNA polymerase and DNA ligase (7).
In contrast to BER, NER is believed to repair bulky, helix-distorting lesions such as bipyrimidine UV photoproducts (5). However, several reports indicate that there is considerable overlap between the DNA excision repair pathways, BER and NER, with respect to damage processing. For example, similar to BER, NER has been shown to be capable of processing oxidative lesions such as thymine glycol and 8-oxoguanine (8-10). In addition, it was demonstrated that both BER and NER repair abasic sites (11, 12). More recently, synergism between BER and NER for the repair of endogenous and exogenous oxidative DNA damage was demonstrated (13).
When excision repair is compromised, it could be expected that a large proportion of spontaneous DNA damage will be left unrepaired and cause deleterious biological consequences. However, in DNA repair defective yeast cells, the level of spontaneous DNA damage existing in the genome has neither been determined nor connected with specific biological endpoints. DNA damage has been implicated in the development of cancer, aging, and other degenerative conditions (14). Although the role of unrepaired DNA damage in mutagenesis and the initiation of carcinogenesis in mammalian cells is well established, its contribution to the progression of tumor development and the acquisition of a genetic instability phenotype is poorly understood, particularly with respect to the levels of DNA damage required to drive these processes (4).
We have employed a series of haploid yeast mutants defective in BER (ntg1 ntg2 apn1), NER (rad1), or BER and NER (ntg1 ntg2 apn1 rad1) to determine the effects of spontaneous DNA damage on cellular processes in S. cerevisiae. As shown previously, it is necessary to disrupt APN1, NTG1, and NTG2 to severely compromise the BER pathway, because single knockouts or double knockout combinations of these three genes do not exhibit obvious phenotypic abnormalities (11). Our results demonstrate that cells acquire remarkably high levels of spontaneous DNA damage that are normally repaired by BER and NER. In addition, we show that, when excision repair is compromised, unrepaired spontaneous DNA damage, upon reaching specific levels, results in gross morphological abnormalities, genetic instability, a chronic stress state characterized by high intracellular ROS levels, profound changes in transcription patterns, and changes in Rad53p phosphorylation. These features are similar to those observed in cancer cells, suggesting that yeast can be used as an informative model for assessing the progressive biological consequences of unrepaired DNA damage in higher eukaryotes and the underlying mechanisms leading to such changes.
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EXPERIMENTAL PROCEDURES |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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ade2-101oc his3
200 ura3Nco lys2Bgl leu2-R). SJR751 will be referred to as wild type (WT). BER-defective strain SJR867 (MAT ade2-101oc his3200 ura3Nco lys2Bgl leu2-R ntgl::LEU2 ntg2::hisG apn11::HIS3), NER-defective strain SJR868 (MAT ade2-101oc his3200 ura3Nco lys2Bgl leu2-R rad1::hisG), BER/NER-defective strain SJR1101 (MAT ade2-101oc his3200 ura3Nco lys2Bgl leu2-R ntgl::LEU2 ntg2::hisG apn11::HIS3 rad1::hisG). Isogenic strains were constructed as described previously (11). We previously observed that the BER/NER-defective strain consistently produced a mixture of large and small colonies when grown on rich YPD medium (11). When these colonies were microscopically examined, small colonies were found to be composed entirely of large cells nearly twice the size of WT cells, whereas large colonies were composed of a mixture of large cells as well as those that were similar in size to WT (Fig. 2A). Therefore, the BER/NER-defective strain was subdivided into two morphological population types, BER/NER-defective mixed cell type or BER/NER-defective large cell type, representing mixed cell size and large cell size populations, respectively. These designations refer to the cell composition of independent colonies from BER/NER-defective strains used to inoculate experimental cultures.
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Cell Growth, Viability, and Microscopy—YPD medium was inoculated with a single yeast colony and grown at 30 °C to a density of 1 x 108 cells/ml. An aliquot was used to inoculate fresh YPD medium to a density of 1 x 104 cells/ml. After 15 h of growth, cells were counted and doubling time was determined. For microscopy and viability assessment, YPD medium was inoculated with a single yeast colony, and cells were grown for 96 h. Cells were harvested and resuspended in fresh YPD medium 48 and 72 h after initial inoculation. 96 h after inoculation, aliquots were streaked onto YPD plates. Plates were placed at 30 °C for 24 h and then viewed at 10x magnification. Approximately 300 of the streaked cells were counted and assessed as viable (formed colony of greater than five cells) or non-viable (failed to form colony of greater than five cells). For microscopy, 1-ml aliquots of cells were harvested 12, 48, 72, and 96 h following initial inoculation. Cells were resuspended in 70% ethanol and 1 µl of 1 mg/ml diaminophenylindole (DAPI) was added. Samples were viewed by microscopy at 100x magnification in both phase contrast and after DAPI staining. For all morphological studies, cells were sonicated prior to viewing to ensure that clumps of cells, composed of several distinct units, were not mistaken for extensively branched single unit networks.
DNA Isolation and Processing—Cells were grown in YPD for 24 h (log phase of growth) or 72 h (stationary phase). After 24 or 72 h of growth, 2 x 109 cells were harvested by centrifugation and resuspended in 0.8 ml of sorbitol EDTA (0.9 M sorbitol, 0.1 M EDTA, pH 7.4) containing 25 µl of dithiothreitol (1 M) and 100 µl of zymolyase 20T (10 mg/ml) (US Biologicals). Cells were incubated at 37 °C overnight. Spheroblasts were spun down, resuspended in 0.5 ml of Tris-EDTA (50 mm Tris-HCl, pH 7.4, 20 mM EDTA) containing 50 µl of 20 mg/ml proteinase K solution (Qiagen), and incubated at 55 °C for 1 h. 100 µl of 10% SDS was added, and samples were incubated for 20 min at 65 °C. After adding 300 µl of potassium acetate (5 M), samples were incubated on ice for 30 min. Samples were centrifuged, supernatants were transferred, and two chloroform extractions were performed. DNA was precipitated with isopropanol, resuspended in TE buffer (10 mM Tris, 1 mM EDTA, pH 8) and digested with 5 µl of ribonuclease A (10 mg/ml) (US Biologicals) at 37 °C for 2 h. Samples were precipitated with ethanol, washed twice, lyophilized, and resuspended in TE buffer. To isolate DNA from cells exposed to H2O2, the same protocol was utilized with the following alterations. 2 x 109 cells were harvested by centrifugation, washed once in sterile water, and resuspended to the initial volume with sterile water. Cells were treated with 2.5 mM H2O2 (Sigma) for 30 min at 30 °C and then harvested by centrifugation and washed twice with sterile water. This DNA extraction procedure was designed to minimize the level of adventitious DNA damage. DNA was quantified by fluorometry using the fluorescent dye bis-benzimide (Hoechst 33258) (Sigma DNA Quantitation kit, DNA-QF). Fluorometry was performed according to the manufacturer's recommendations. 10-µg aliquots of yeast DNA were digested with BamH1 and ApaL1 (New England Biolabs), using 2.5-3 units of each enzyme per microgram of DNA, for 4 h at 37 °C. Digestion with these enzymes yields a 3.7-kb fragment containing the 1.8-kb coding sequence of the CAN1 gene and 1.9 kb of flanking sequence on chromosome V. After restriction, DNA was precipitated with ethanol, washed twice, lyophilized, and resuspended in TE buffer to a concentration of 1 µg/µl. DNA concentration of the digested samples was determined by the average of at least five independent fluorometric readings.
Alkaline Gel Electrophoresis—Gel electrophoresis was performed using a 1.1% alkaline agarose gel in alkaline electrophoresis buffer (30 mM NaOH, 2 mM EDTA, pH 8) for 20 min at 40 V and then for 15 h at 17 V (17). Genomic DNA samples were prepared as follows. 10 µg of BamH1- and ApaL1-digested genomic DNA was incubated with 5 µg of recombinant glutathione S-transferase-tagged NTG1 protein in a 25-µl reaction mixture containing buffer B (15 mM KH2PO4, pH 6.8, 10 mM EDTA, 10 mM 
-mercaptoethanol, and 40 mM KCl) (6). All sample volumes were equalized by adding TE and then incubated for 30 min at 37 °C. To stop the reaction, samples were heated at 60 °C for 5 min. An internal standard corresponding to a 710-bp segment of the CAN1 gene, generated by PCR amplification, was added to each sample, after Ntg1p incubation, at a concentration of 0.15 ng/µg of genomic DNA. Prior to loading, denaturing dye (50 mM NaOH, 1 mM EDTA, pH 8) was added to each sample. The recombinant Ntg1p used in these experiments is N-terminally tagged with glutathione S-transferase and expressed in Escherichia coli as described previously (6). The protein was purified by glutathione-agarose chromatography followed by Mono-S fast-performance liquid chromatography (18).
Transfer of DNA to Membranes and Hybridization—Transfer and hybridization were performed according to Sambrook and Russell (19), with the following modifications. Transfer was performed utilizing alkaline transfer buffer (20x SSC, 0.4 M NaOH). After 24 h of transfer, DNA was UV-cross-linked to the nylon membrane and pre-hybridized at 65 °C in rapid-hyb buffer (Amersham Biosciences). For hybridization, a 423-bp PCR-generated fragment of the CAN1 gene was the template for a random primed [-32P]dATP-labeled probe. The amount of DNA in the 3.7-kb CAN1 gene fragment was determined by PhosphorImager analysis of the Southern blot and used to determine levels of oxidative DNA damage. All CAN1 fragment bands were normalized to corresponding internal standard bands to control for sample loading variations. The Poisson expression was used to calculate the number of NTG1p-recognized lesions per 3.7-kb CAN1 fragment (20).
Detection of Rad53 by Western Blotting—Yeast protein extracts were prepared following zirconium bead lysis in trichloroacetic acid and Rad53 was visualized by PAGE gel electrophoresis as described previously (15, 16). The primary Rad53 antibody used was from Santa Cruz Biotechnology. For phosphatase treatments, the trichloroacetic acid precipitate was washed twice in acetone, resuspended in phosphatase buffer (Roche Applied Science) and incubated for 30 min at 37 °C with 10 units of calf intestine phosphatase (Roche Applied Science).
Measurement of Spontaneous Mutation and Recombination Frequencies—YPD media was inoculated with a single colony and cells were grown to saturation. With an aliquot of this culture, fresh YPD media was inoculated to a density allowing harvest of 2 x 107 cells/ml after 15 h of growth. To assess CanR mutations and Lys+ recombinants, cells were harvested and plated as described (11). To identify lys2Bgl revertants, the following modifications were made. Cell aliquots were plated onto complete YNBD media to determine initial viability. The remaining cells were resuspended in YPD media and allowed to grow for 24 h at 30 °C. Cells were then harvested and plated onto complete YNBD medium to determine viable cell number in the outgrowth population. Remaining cells were plated onto YNBD medium lacking lysine to select for Lys+ revertants. The lys2Bgl mutation frequency values were corrected for the number of generations undergone during outgrowth. Both CanR and Lys+ colonies were counted 3 days after selective plating. The mutation and recombination frequencies reported in Table II were calculated as follows: the number of mutants or recombinants/total number of colonies. Potential jackpot cultures were identified as outliers statistically by using the Grubbs test (21). These values were withheld from frequency calculations presented in Table II. The data from a minimum of 20 independent cultures was used for each mutation and recombination frequency determination.
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5 x 106 cells/ml was centrifuged for 5 min at 3020 rpm. The supernatant was removed, and cells were resuspended in 1 ml of water. Cells were centrifuged again, and the supernatant was removed. The resulting pellet was resuspended in 1 ml of absolute ethanol and stored at 4 °C overnight. Cells were vortexed vigorously, centrifuged, resuspended in 1 ml of water, and pelleted again. The pellet was then resuspended with 1 ml of 50 mM sodium citrate (pH 7.0) and transferred to appropriate FACS analysis tubes (Falcon 2054, 12 x 75 mm). 8 µl of 10 mg/ml DNase-free RNase A was added, and samples were incubated for 1 h at 50 °C. 25 µl of 10 mg/ml of proteinase K was added, and the samples were incubated for 1 h at 50 °C. Samples were then stained with 1 ml of propidium iodide (16 µg/ml) in 50 mM sodium citrate (pH 7.0). Samples were shielded from light and analyzed using a BD Biosciences FACSCalibur® and CellQuest® software. Reactive Oxygen Species Levels—ROS levels were measured with dihydrorhodamine 123 according to Qi et al. (22), with the following modifications. 5 ml of mid-log phase cell culture was treated with 2.5 µg/ml dihydrorhodamine 123 for 2 h at 30 °C. The cell culture was washed and resuspended in PBS and analyzed using a FACSCalibur® and CellQuest® software. Wild type cells were exposed to 3 or 4 mM H2O2 or left untreated for 30 min at 30 °C. Cells were washed once, resuspended in media, and then treated with dihydrorhodamine 123 for ROS assessment.
Microarray Analysis of Gene Expression—Cell cultures were monitored for growth until they reached 5 x 106 cells/ml and were exposed to 0.75 mM H2O2 or left untreated for 30 min at 30 °C. Following treatment, cells were harvested and total RNA was isolated. Total RNA was extracted from cells using the hot-phenol method. RNA was then purified on an RNA affinity resin (RNeasy Mini Kit, Qiagen, Chats-worth, CA). The total RNA was ethanol-precipitated and resuspended in 15 µl of diethyl pyrocarbonate-treated water (RNase-free). Total RNA was quantitated by UV spectrophotometry. Approximately, 20 µg of total RNA was used in the cDNA synthesis reaction. The cDNA synthesis procedure was carried out as described in the Affymetrix GeneChip Expression Analysis Technical Manual, using a modified oligo(dT) primer with a T7 RNA polymerase promoter on the 5'-end of the sequence (5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(dT)24-3') and Invitrogen reagents. cDNA was ethanol-precipitated and resuspended in 12 µl of diethyl pyrocarbonate water. 3.3 µl of cDNA was used for the cRNA biotin-labeling reaction. cRNA was labeled with Bio-UTP and Bio-CTP as directed in the ENZO Bioarray High Yield RNA transcript labeling kit (ENZO Diagnostics, Farming-dale, NY). cRNA was purified on an affinity resin (RNeasy Mini Kit, Qiagen) and was fragmented as described in the Affymetrix GeneChip Expression Analysis Technical Manual. Fragmented cRNA was quantitated by measuring absorbance at 260 nm, and purity was ascertained by determining the absorbance ratio A260 nm/A280 nm. All RNA samples yielded ratios of 1.9 or greater indicating appropriate RNA quality.
Chip Hybridization and Data Analysis—The hybridization mix was prepared using 25 µg of fragmented cRNA and reagents as recommended by the manufacturer to a final volume of 300 µl. The hybridization mix was heated at 99 °C for 5 min and then 45 °C for 5 min. Following a 5-min centrifugation, 200 µl of the hybridization mixture was loaded onto YSG98 microarray chips (Affymetrix, Santa Clara, CA) containing 6400 ORFs.C. O. Following hybridization, the chips were loaded into the fluidics station for washing and staining as described in Section 2, Chapter 4 of the Affymetrix GeneChip Expression Analysis Technical Manual. After staining and washing, the chips were loaded into the GeneArray Scanner and scanned using a specialized confocal laser scanning microscope and analyzed using Microarray Suite version 5.0. The arrays were scaled to have a target intensity of 150 in order for all chips to be compared with one another. Integrity of RNA samples was determined by comparing the signal intensity of probes derived from the 5'-end and the 3'-end of actin and the TATA-binding protein. The signal ratio 5' to 3' did not exceed 2.5-fold, indicating that the mRNA was not degraded during preparation. For all chips, Affymetrix Statistical Algorithms were applied in the analysis of the probe arrays as described in the Statistical Algorithms Reference Guide (obtainable from Affymetrix, Santa Clara, CA, available at www.Affymetrix.com). Expression information can be found on the Doetsch laboratory website (www.biochem.emory.edu/labs/medpwd/microarray_index.html).
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RESULTS |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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Genomic DNA from WT- and NER-defective cells in log phase of growth revealed no reduction of the 3.7-kb CAN1 gene segment following incubation with Ntg1p, whereas DNA from BER-defective cells showed a modest reduction (Fig. 1 and Table I). In contrast, genomic DNA extracted from both BER/NER-defective mixed and large type cells in log phase of growth contained significant levels of unrepaired oxidative DNA damage recognized by Ntg1p, resulting in a reduction of 23 and 35% in the CAN1 signal, respectively (Fig. 1 and Table I). Using the Poisson formula to estimate DNA damage, the frequency of Ntg1p-recognized DNA lesions per 3.7-kb CAN1 gene fragment was calculated (20). The number of lesions per fragment was negligible in WT cells, 0.26 in BER/NER-defective mixed type cells, and 0.43 in BER/NER-defective large type cells, corresponding to the presence of 840 and 1400 Ntg1p-recognized lesions per genome, respectively (Table I). The level of oxidative base damage induced in WT cells by an acute exposure to H2O2 (2.5 mM), resulting in 58% killing, corresponds to 1360 Ntg1p-recognized lesions per genome (Fig. 1 and Table I). After 72 h of growth (stationary phase), an increase in Ntg1-recognized DNA lesions was observed for all strains (Table I). This result is consistent with the observation that more ROS are present in stationary phase yeast cells (24). The finding that the BER/NER-defective strain contained such high levels of genomic oxidative DNA damage is remarkable and indicates that these cells are capable of tolerating substantial, chronic spontaneous DNA damage. In addition, these measurements allow for the connection of a particular level of unrepaired DNA damage with associated deleterious biological consequences as described below.
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In addition to displaying abnormal cellular and colony morphology, BER/NER-defective cells (both mixed cell type and large cell type) grew at 50% (180 min per generation) of the rate of WT cells (data not shown). Because the growth rates of the BER/NER-defective strain types were substantially retarded, the viability of this strain after 96 h of growth in rich media was determined. WT, BER-defective, and NER-defective cells exhibited less than a 5% decrease in viability over 96 h of continuous growth, whereas BER/NER-defective cells showed a marked decrease in viability, with mixed cell type and large cell type exhibiting decreases of 20 and 40%, respectively (data not shown). Such a decrease in viable, colony-forming cells can be observed in microscopic fields containing large numbers of single, non-viable BER/NER-defective cells (Fig. 2B). Thus, adverse effects on cellular and colony morphology, cell growth, and viability accompany deficiencies in excision repair.
Increased DNA Content in Excision Repair-defective Cells—The observed slow growth and aberrant cellular morphology, especially the large cell size, of the BER/NER-defective strain could reflect abnormalities in cell cycle progression. To address whether BER/NER-defective cells possess altered cell cycle properties, cells in log phase of growth were subjected to FACS analysis. WT, BER-defective, and NER-defective cells show a normal distribution of G1 and G2/M cells as evidenced by two peaks (Fig. 3A). The profile for the BER/NER-defective strain, however, shows a profound shift and broadening of these two peaks. Such a peak profile is similar to those that have been previously shown to correspond to cells with increased DNA content and size (29). The DNA content profile and the large cell size of the BER/NER-defective large cell type suggest that this strain possesses a 2N or greater DNA content at all times. This was confirmed by isolating DNA from equal numbers of WT, BER-defective, NER-defective, and BER/NER-defective mixed and large type cells. The DNA content per cell of the BER/NER-defective large cell type was significantly higher (2.5- to 4-fold) than that of the other strains (Fig. 3B). Attempts to monitor cell cycle progression were unsuccessful because of the exquisite sensitivity of the BER/NER-defective cell types to the toxic effects of nocodazole and hydroxyurea (data not shown). However, the sensitivity of the BER/NER-defective strain to these cell cycle checkpoint drugs indicates that these cells may not respond properly to checkpoint controls, regardless of which stage of the cell cycle these inhibitors are acting upon (30).
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Bgl frameshift allele. Forward mutations in the arginine permease gene CAN1 render the locus non-functional and the cells resistant to the toxic arginine analog L-canavanine (31). A 3N-1 frameshift mutation within a 150-bp reversion window in the lys2Bgl allele yields Lys+ prototrophs (32). For recombination measurements, recombination between two non-functional LYS2 alleles, lys2Bgl on chromosome II and lys23500 on chromosome V, was measured by Lys+ prototroph production (33). Previous studies revealed significant increases in both spontaneous mutation and recombination rates of the BER/NER-defective strain over WT in stationary (non-dividing) phase (11). However, because abnormal cellular morphology was first observed during log phase of growth in the BER/NER-defective strain, but not the WT, BER-defective, or NER-defective strain, mutation and recombination frequency status during log phase of growth was determined. Both mutation and recombination frequencies were moderately elevated in the BER-defective strain compared with the WT and NER-defective strains (Table II). In contrast, when BER and NER are simultaneously compromised, mutation and recombination frequencies increase synergistically. The BER/NER-defective strain was increased 56- and 48-fold over WT in CanR and lys2Bgl mutation frequencies, respectively, and 121-fold above WT in recombination frequency. Such increases in mutation and recombination frequencies suggest that when DNA excision repair is compromised, the remaining unrepaired, spontaneous DNA damage is accompanied by increases in at least two types of genetic instability.
Elevated ROS in Excision Repair-defective Cells—ROS are produced through normal cellular metabolic processes or exposure to stress agents and are thought to be important for signal transduction pathways associated with control of transcription and cell growth (34, 35). To determine whether unrepaired spontaneous DNA damage leads to an increase in ROS, log phase cells were grown in the presence of dihydrorhodamine, an intracellular ROS indicator (22). The WT, BER-defective, and NER-defective strains have identical profiles for intracellular ROS content, whereas the BER/NER-defective strain has 29% (mixed cell type) and 56% (large cell type) of cells with substantially higher intracellular ROS levels compared with the other strains (Fig. 5A). These results indicate that unrepaired spontaneous DNA damage leads to an increase in intracellular ROS. In addition, the BER/NER-defective large cell type ROS profile is intermediate to that produced by exposures to 3 and 4 mM H2O2 in the WT strain (Fig. 5B). These results indicate that the BER/NER-defective strain has adapted to survive under conditions of oxidative stress, which, when given to WT cells as an acute exposure to H2O2, produce similar intracellular ROS levels that are accompanied by a high degree of toxicity.
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50% killing, the expression pattern also varied significantly from the untreated WT expression pattern (Fig. 6E). There was 21% overlap in the genes up-regulated in the BER/NER-defective large type cells compared with WT cells exposed to a toxic dose of H2O2. Such genes include UBI4 (elevated 3.3-fold), encoding ubiquitin, which has been shown to be necessary for regulation of DNA repair activities (36), and GPM2 (elevated 4.5-fold), identified as being induced by a number of known DNA damaging agents (37-39). Specifically, 122 genes are up-regulated (2- to 289-fold), and 13 genes are down-regulated (2- to 254-fold) in the BER/NER-defective large type cells when compared with WT cells (no H2O2 exposure) (data represent three independent experiments). All expression data information can be found at www.biochem.emory.edu/labs/medpwd/microarray_index.html.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
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In log phase of growth (24 h), we have shown that WT and NER-defective cells have negligible levels of Ntg1p-recognized lesions. This finding is somewhat surprising considering that NER repairs a number of spontaneously occurring Ntg1p-recognized lesions (8-12). In contrast, BER-defective cells possess moderately elevated levels of DNA damage, 400 Ntg1p-recognized lesions per genome (Table I). Such unrepaired DNA damage in the BER-defective strain results in no obvious deleterious effects with respect to growth, viability, morphology, cell cycle, intracellular ROS, and global gene expression patterns but does cause a moderate increase in mutation and recombination frequencies (Table II). Therefore, spontaneous levels of DNA in the range of 400 Ntg1p-recognized lesions per genome cause genetic instability but no other observable deleterious biological consequences.
In contrast to the single pathway-defective strains, BER/NER-defective cells in log phase of growth (24 h) have dramatically elevated levels of DNA damage (1400 Ntg1p-recognized lesions per genome) and consequently exhibit profound biological and genetic changes. The BER/NER-defective strain displays characteristics similar to cancer cells, such as genetic instability, abnormal growth, morphological aberrations, increased levels of intracellular ROS, and altered global transcription profiles (4). Significantly, these results establish a range of greater than 400 but less than 1400 Ntg1p-recognized lesions per genome necessary to provoke adverse biological effects. However, because Ntg1p recognizes only a subset of DNA lesions resulting from spontaneous processes, our measured DNA damage frequencies represent an underestimate of the total number of lesions present per genome. Our system does, however, demonstrate that as spontaneous DNA damage accumulates there is a progression of deleterious consequences beginning with genetic instability followed by other biological abnormalities. Levels of spontaneous DNA damage, in the range of 400 lesions per genome, result in genetic instability, but no other biological consequences. However, when spontaneous DNA damage levels reach 1400 lesions per genome, a constellation of additional biological changes occur.
Despite the significantly elevated spontaneous DNA damage levels in the BER/NER-defective strain in log phase of growth, activation of the Rad53p DNA damage checkpoint pathway does not occur. However, when BER/NER-defective cells enter stationary phase, DNA damage levels increase and Rad53p becomes phosphorylated. This suggests that Rad53p phosphorylation status is dependent upon the amount of unrepaired oxidative DNA damage present in cells. This also suggests that there is a narrow threshold of DNA damage that needs to be exceeded for Rad53p checkpoint activation (in our study, between 1400 and 1900 Ntg1p-recognized lesions per genome). Because Rad53p is autocatalytic, this supports our observation that a narrow threshold of DNA damage is needed for its activation (40). In addition, checkpoint activation in stationary phase (non-dividing) cultures suggests that unrepaired DNA damage triggers checkpoint activation rather than interference with replication or a DNA repair intermediate, although we cannot rule out a contribution from recombination repair. Similar observations have been made for UV-induced DNA damage (15). In humans, repair-proficient senescent cell cultures are characterized by phosphorylated Chk2 (the human Rad53p homolog), presumably due to telomere erosion (41). However, our results indicate that oxidative DNA damage may also play a role in regulation of senescence. The absence of Rad53p phosphorylation during the growth phase of a culture could reflect a lower DNA damage level. Alternatively, the absence of Rad53p phosphorylation could represent a need to actively dampen the DNA damage signal to allow for replication in the presence of unrepaired DNA damage. This process, known as adaptation, is clearly correlated with reduction of Rad53p phosphorylation in budding yeast (42). A silenced checkpoint system could also provide an explanation for our inability to synchronize the BER/NER-defective cells with the checkpoint-triggering agents nocodazole and hydroxyurea.
Because the only difference between the WT and the BER/NER-defective strain is in the ability to repair spontaneous DNA damage, global genome expression analysis allowed identification of responses resulting specifically from unrepaired, spontaneous DNA damage present in the genome of BER/NER-defective cells. Because Rad53p phosphorylation was not detected in BER/NER-defective cells in log phase of growth, these DNA damage-dependent changes in transcription do not involve the Rad53p-dependent DNA damage checkpoint pathway. However, the up-regulation of RNR3 and HUG1 in BER/NER-defective cells supports the idea that DNA damage signaling is occurring. For example, RNR3 is maintained at low levels in wild type yeast and is induced only upon signaling resulting from DNA damage (43). Signaling in response to DNA damage is dependent on Mec1p (44), which causes a number of changes, including phosphorylation of proteins involved in DNA damage responses (25). HUG1, which suppresses mec1 lethality (suggesting HUG1 is a negative regulator of MEC1) is induced following activation of Mec1p (45). In our study, significant up-regulation of HUG1, a 20-fold increase over WT, was observed in BER/NER-defective cells. Consequently, to allow for survival, we speculate that HUG1 may be highly up-regulated to suppress the continuous DNA damage signal/response occurring in BER/NER-defective cells. Furthermore, 64% of the genes up-regulated in the BER/NER-defective cells have been identified in previously published transcriptional studies as being up-regulated by exposure to exogenous DNA damaging agents, including methylmethane sulfonate, ionizing radiation, and H2O2 (37-39). These studies support the notion that many changes in expression in the BER/NER-defective cells are due to responses to unrepaired, spontaneous DNA damage. Therefore, the 122 genes identified in this study not only represent candidate responders to DNA damage but may also represent genes that are necessary for survival and growth under conditions of chronic oxidative stress caused by DNA damage. As a result, further analysis of these strains, under conditions of acute exposure to DNA-damaging agents, may be useful for identifying genes responding specifically to the presence of DNA damage as opposed to other signaling pathways initiated outside of the nucleus.
Our findings also show that unrepaired, spontaneous DNA damage in BER/NER-defective cells results in increased levels of intracellular ROS. It has been observed that changes in ROS levels are accompanied by changes in signal transduction pathways controlling transcription and cell growth (34, 35). The increased levels of ROS in the BER/NER-defective cells may indicate changes in signal transduction pathways involved in yeast cell survival when DNA damage levels are chronically elevated and left unrepaired. In human cells, it has been observed that, upon p53 pathway activation, which occurs in response to DNA damage, levels of ROS increase (35). The increased ROS levels may also indicate changes in the regulation of proteins through post-translational modifications necessary for repair of DNA damage. For example, RNR3, which is a component of ribonucleotide reductase, is regulated by its redox state. To produce dNTPs, which are necessary for replication and to execute DNA repair or damage tolerance, ribonucleotide reductase activity is up-regulated through redox signaling during S phase and in response to DNA damage (43).
In the BER/NER-defective cells, the abnormal cellular and colony morphologies, due to unrepaired, spontaneous DNA damage, are reminiscent of environmental stress-induced pseudohyphal growth and the morphologies of cells with mutations in cell cycle regulators CDC28 and cyclins (27, 46). These morphologies are also consistent with those initiated by MEC1 in response to slowed DNA synthesis in haploid yeast (28). Although the above study failed to show induction of filamentous growth by sublethal doses of DNA-damaging agents, our results reveal that the accumulation of unrepaired, spontaneous DNA damage elicits a similar filamentous phenotype. These similarities, as well as the documented growth delays, cell size enlargement, and increased DNA content of the BER/NER-defective cells, suggest that these cells possess cell cycle abnormalities. This idea is further supported by their extreme sensitivity to DNA damage checkpoint drugs. However, because Rad53p phosphorylation was detected in BER/NER-defective cells in stationary phase, this suggests that in mid-log phase of growth, either the Rad53p checkpoint pathway is silenced or another cell cycle checkpoint pathway is corrupted. Together, these results indicate that acquisition of specific levels of unrepaired, spontaneous DNA damage not only results in genetic instability but also profoundly affects global gene expression patterns, intracellular ROS levels, and cell cycle properties. Below these levels of DNA damage, such deleterious biological endpoints do not occur.
We have shown, in yeast, that when specific DNA repair pathways are corrupted, a particular level of spontaneous DNA damage accumulates. The resulting genetic instability and loss of biological integrity observed in our strains are analogous to cellular phenotypes of human disease states such as cancer. For example, cancer cells have been shown to accumulate oxidative DNA damage and display elevated levels of intracellular ROS, polyploidy, genetic instability, aberrant cellular morphologies, and transcriptional alterations (4, 14, 47). In addition to cancer, cells from aged tissue and neurodegenerative diseases accumulate oxidative DNA damage (14). These biological and genetic effects might also occur in cells, such as those affected by chronic inflammation, where conditions of continuous oxidative stress may saturate one or more functional DNA excision repair pathways. We have generated yeast strains possessing different levels of oxidative stress and DNA damage due to deficiencies in one or two DNA excision repair pathways and have established the levels of Ntg1p-recognized lesions that, together with other types of spontaneous DNA damage, are responsible for provoking deleterious biological consequences. Given the similarity of phenotypes observed in several human disease states and our yeast DNA repair mutants, we propose that such strains might be exploited as an informative model system for gauging the sequential biological consequences of chronic oxidative stress and DNA damage accumulation.
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FOOTNOTES |
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¶ Both authors contributed equally to this work.
To whom correspondence should be addressed: Dept. of Biochemistry, Rollins Research Center, Rm. 4013, 1510 Clifton Rd., Atlanta, GA 30322. Tel.: 404-727-0409; Fax: 404-727-2618; E-mail: medpwd{at}emory.edu.
1 The abbreviations used are: BER, base excision repair; NER, nucleotide excision repair; MMR, mismatch repair; DAPI, diaminophenylindole; FACS, fluorescence-activated cell sorting; ROS, reactive oxygen species; ORF, open reading frame.
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