| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 279, Issue 30, 31638-31645, July 23, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
-converting Enzyme by Its Pro Domain*
||**
From the
Department of Biochemistry and Biophysics and Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6059, the Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel, and ¶GlaxoSmithKline Research and Development, Research Triangle Park, North Carolina 27709
Received for publication, February 5, 2004 , and in revised form, April 14, 2004.
 |
ABSTRACT |
|---|
 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
-converting enzyme (TACE) is a disintegrin metalloproteinase that processes tumor necrosis factor and a host of other ectodomains. TACE is biosynthesized as a zymogen, and activation requires the removal of an inhibitory pro domain. Little is known about how the pro domain exerts inhibition for this class of enzymes. To study the inhibitory properties of the pro domain of TACE, we have expressed it in isolation from the rest of the protease. Here we show that the TACE pro domain (TACE Pro) is a stably folded protein that is able to inhibit this enzyme. TACE Pro inhibited the catalytic domain of TACE with an IC50 of 70 nM. In contrast, this inhibitory potency decreased over 30-fold against a TACE form containing the catalytic plus disintegrin/cysteine-rich domains (IC50 greater that 2 µM). The disintegrin/cysteine-rich region in isolation also decreases the interaction of TACE Pro with the catalytic domain. Surprisingly, we found that the cysteine switch motif located in TACE Pro was not essential for inhibition of the enzymatic activity of TACE; the pro domain variant C184A showed the same inhibitory potency against both TACE forms as wild type TACE Pro. X-ray absorption spectroscopy experiments indicate that binding of TACE Pro to the catalytic domain does include ligation of the catalytic zinc ion via the sulfur atom of its conserved Cys184 residue. Moreover, the binding of TACE Pro to the catalytic zinc ion partially oxidizes the catalytic zinc ion of the enzyme. Despite this, the nature of the interaction between the pro and catalytic domains of TACE is not consistent with a simple competitive model of inhibition based on cysteine switch ligation of the zinc ion within the active site of TACE. |
INTRODUCTION |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
-converting enzyme (TACE1 or ADAM 17) is a zinc metalloproteinase that cleaves the precursor, membrane-bound form of tumor necrosis factor- and other ectodomains (1–3). TACE is a member of a family of proteases known as the ADAMs (a disintegrin and metalloproteinase). ADAMs are multidomain proteins typically comprising a pro domain, metalloproteinase domain, a disintegrin/cysteine-rich domain, transmembrane domain, and a cytoplasmic tail (4, 5). ADAMs are synthesized as latent precursors and later converted to the mature enzyme, lacking the pro domain, by furin or a furin-like enzyme in the secretory pathway (6, 7).
It has been shown previously that the pro domain of TACE seems to act as an inhibitor of this enzyme, because the activity of TACE was only recovered upon its removal (8). TACE Pro includes a cysteine switch box (PKVCGY186), a feature present in most metzincins, including matrix metalloproteinases and ADAMs. It has been proposed that the pro domains of metzincins act as inhibitors of their catalytic domains through a mechanism that involves ligation of the cysteinyl thiol within the cysteine switch box to the zinc ion in the active site (9–11). The cysteine switch present in TACE Pro did appear to be important for inhibition of this enzyme, because thiol-modifying reagents such as 4-aminophenylmercuric acetate and octylthioglucoside promoted pro domain release from the catalytic domain and, therefore, enzyme activation (8).
TACE Pro also serves a second function: it is essential for the secretion of functional enzyme. In insect cells, a recombinant form of this enzyme lacking the pro domain failed to be secreted and was extensively degraded intracellularly (8). Similar results have been reported for other members of the ADAM family (12, 13). At first approximation, this is similar to the secreted bacterial serine proteases subtilisin and -lytic protease, in which the pro domain accelerates the folding of the catalytic domain by several orders of magnitude, apparently by lowering the conformational energy barrier between the unfolded and native states (14–17).
Here we show that the isolated pro domain of TACE is a potent inhibitor of the catalytic domain of this enzyme. TACE Pro is a less effective inhibitor of a form of TACE comprising the catalytic plus disintegrin/cysteine-rich domains, suggesting a role for the disintegrin/cysteine-rich domain in pro domain removal and enzyme activation. We also show that the cysteine switch of TACE, although present in the zymogen form, is not required for the interaction between the pro and catalytic domains of TACE.
|
MATERIALS AND METHODS |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
Expression of Recombinant TACE Pro Proteins—E. coli BL21(DE3) electrocompetent cells were transformed with the corresponding TACE Pro plasmid and plated on LB plates containing 150 µg/ml ampicillin. After overnight incubation at 37 °C, the cells were resuspended in 1 liter of LB with 150 µg/ml ampicillin. The cells were grown at 37 °C, induced with 1 mM isopropyl-
-D-thiogalactopyranoside at an optical density of 0.6 and harvested 3 h after induction.
Purification and Refolding of Wild Type and Mutant TACE Pro—The cell pellets were washed once with 20 mM Tris-HCl, pH 8, and twice with the same buffer containing 0.1% Triton X-100. Washed pellets were solubilized in 20 mM Tris-HCl, pH 8, containing 6 M Gdn-HCl and centrifuged at 26,000 x g for 30 min. The supernatant was applied to a 20-ml Ni+2-nitrilotriacetic acid column. The column was washed with 20 mM Tris-HCl, pH 8, 6 M Gdn-HCl and then with the same buffer containing 20 mM imidazole. The protein was eluted with 20 mM Tris-HCl, pH 8, 6 M Gdn-HCl, 300 mM imidazole. The eluate was dialyzed against water. Under these conditions, the protein formed a white precipitate that was then resuspended in 20 mM Tris-HCl, pH 8, 6 M Gdn-HCl to a concentration of 6 mg/ml. 50 µl of this protein solution were incubated with 950 µl of refolding buffer (FoldIt Screen Formulation 16; Hampton Research) with addition of 1 mM reduced glutathione, 0.1 mM oxidized glutathione, and 3 mM lauryl maltoside for 4 h at 4 °C with gentle stirring. After refolding, the solution was centrifuged at 10,000 x g for 10 min and filtered through a 0.22-µm filter. Finally, it was dialyzed against 250 ml of 20 mM Tris-HCl, pH 8, with 150 mM NaCl (buffer A). The final protein concentration obtained after this procedure was 4–6 µM (0.09–0.14 mg/ml).
Expression and Purification of TACE Truncates R473 and R651— The construction of TACE truncates R473 (Met1–Arg473) and R651 (Met1–Arg651) and production of baculovirus in Sf9 (Spodoptera frugiperda) cells for protein production in insect cells have been described previously (8). Logarithmically growing Trichoplusia ni cells were infected with baculovirus strains encoding R473 or R651 at a multiplicity of infection of 1. The cultures were harvested 48 h after infection, and the proteins were purified as described previously (8).
TACE Activity and Inhibition Assays—The inhibitory activity of TACE Pro was determined by an high performance liquid chromatography-based assay using the synthetic peptide dinitrophenyl-SPLAQAVRSSSR-NH2 as the substrate. TACE (R473 and R651), at a final concentration of 1 nM, was incubated with a series of TACE Pro concentrations ranging from 0 to 6 µM in buffer A. The incubation took place for 20 min at 37 °C with gentle shaking. The reaction was initiated by the addition of substrate at a final concentration of 20 µM. The reactions were incubated at 37 °C for 30 min with gentle shaking, quenched by the addition of an equal volume of 1% heptafluorobutyric acid, and filtered using polyvinyl difluoride membrane filters. The quenched reaction mixtures were applied to a 150-mm C18 column (Vydac) and resolved using a gradient of 0.1% heptaflurobutyric acid in water and 0.1% heptaflurobutyric acid in acetonitrile. The ratio of product/substrate was obtained by integration of the absorbance of the resolved peaks at 350 nm.
Circular Dichroism Spectroscopy—CD spectra were recorded in an AVIV 62DS spectropolarimeter. Protein stocks were maintained at a concentration of 180 µM in buffer A containing 6 M Gdn-HCl. Those stocks were rapidly diluted 60-fold in buffer A to reach a final concentration of 3 µM of refolded protein. The spectra were taken between 208 and 260 nm using a 1-cm-path fused quartz cuvette. Scans were done in triplicate.
For denaturation studies, the protein stocks were diluted into buffer A containing Gdn-HCl concentrations ranging from 0 to 7.5 M. The ellipticity at 222 nm was recorded for four independent protein dilutions at each Gdn-HCl concentration.
Fluorescence Spectroscopy—The fluorescence emission scans were obtained using an AVIV ATF-105 spectrofluorometer. The samples were prepared in the same way as for CD spectroscopy. The protein samples were excited at 280 nm, and the intrinsic tryptophan emission spectra were recorded from 300 to 400 nm using a 3-mm-path cuvette. The photomultiplier tube was preset to 50% of the maximum output at a fixed excitation wavelength of 280 nm and an emission wavelength of 350 nm.
For denaturation studies, the protein stocks were also diluted in buffer A containing a range of Gdn-HCl concentrations from 0 to 7.5 M. The emission spectra were recorded from 300 to 400 nm, and the center of mass of each spectrum (
c) was calculated using the equation,
 | (Eq. 1) |
i. Sample Preparation for X-ray Absorption Spectroscopy Studies—The catalytic domain of TACE was concentrated by ultra filtration using Vivaspin 6-ml units (Vivascience AG; 10-kDa cut-off) to a final concentration of 0.1 mM (2.92 mg/ml). The samples were loaded into copper sample holders (10 x 5 x 0.5 mm) precovered with Mylar tape, which is transparent to X-rays, immediately followed by freezing in liquid nitrogen. The frozen samples were then mounted inside a Diplex closed cycle helium cryostat, and their temperature was maintained at 30 K to minimize thermal disorder in the XAS data.
XAS Data Collection—Data collection was performed at the National Synchrotron Light Source at Brookhaven National Laboratory, beam line X9B. The spectra were recorded at the zinc K-edge (zinc absorption edge energy in which a 15-core electron is excited by the x-ray beam) in fluorescence geometry at a low temperature (30 K). The beam energy was defined using a flat Si (111) monochromator crystal. The incident beam intensity I0 was recorded using an ionization chamber. The fluorescence intensity was recorded using a 13-element germanium detector. The transmission signal from a zinc foil was measured with a reference ion chamber simultaneously with fluorescence to calibrate the beam energy position throughout all measurements. Five or six scans of each sample were collected. The samples were checked for burning marks after each scan, and the beam position on the sample was changed before each scan to minimize radiation damage. The enzyme integrity was checked before and after exposure to X-rays. In these experiments, TACE was found to be intact and fully active.
XAS Data Processing and Analysis—The average zinc K-edge absorption coefficient µ(E), was obtained after averaging 5–13 independent XAS measurements for each sample. Each data set was aligned using the first inflection point of a reference zinc metal foil (9659 eV). Subsequently, the absorption coefficients for different samples were shifted in x-ray energy until their first inflection points were aligned at the same energy.
The smooth atomic background was then removed with the AUTOBK program of the UWXAFS data analysis package, developed at the University of Washington at Seattle (18). For background removal, the energy shift, E0, was chosen as the edge shift of the processed data for each sample and used as the origin of the photoelectron energy. The R space region for minimizing the signal below the first shell was chosen between 0.6 and 1.2 Å. Upon background removal, the useful k range (energy range used in fitting procedure, in wave units) in the resultant k2-weighted (k) was between 0 and 10.0 Å-1. Model data for fitting procedures were constructed by extracting the structural zinc site coordinates (in a radius of 4 Å from the zinc) of gelatinase A (Protein Data Bank code 1CK7 [PDB] ) and stromelysin-1 (Protein Data Bank code 1SLM [PDB] ). The theoretical photoelectron scattering amplitudes and phase shifts were calculated for each zinc ligand (path), using the computer code FEFF7 (19, 20). The total theoretical signal (k) was constructed by adding the most important partial k values that contributed to the R range of interest. The theoretical XAFS signal was fitted to the experimental data using the nonlinear least squares method, implemented in the program FEFFIT 2.98 (18) in R space, by Fourier transforming of both theoretical and experimental data. The experimental data and theoretical values were weighted by k and multiplied by a Hanning window function in Fourier transforms.
|
RESULTS |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
TACE Pro was expressed at high levels and accumulated as inclusion bodies. The purification protocol involved a nickel chelate chromatography step under denaturing conditions followed by refolding via dialysis. Refolding of TACE Pro was the limiting step in obtaining large amounts of the protein, because of its tendency to aggregate at concentrations over 8 µM. Nevertheless, the final yield for this refolding step was typically 31–48%. After refolding, TACE Pro was over 95% pure judging by reducing SDS-PAGE analysis. The protein partitioned between two forms: a free monomer and a disulfide-linked dimer (Fig. 1). The free monomer constituted 40% of the purified protein, as determined by densitometric analysis of gels ran at different total protein concentrations.
|
|
|
|
|
An Intact Cysteine Switch Is Not Required for Inhibition— The pro domain of TACE contains a conserved cysteine residue at position 184 in the context of a cysteine switch consensus motif. This residue was expected to mediate inhibition via coordination to the zinc ion in the active site, as proposed in the cysteine switch model (9–11). To study the importance of the cysteine switch motif in the inhibition of TACE, we generated a variant of TACE Pro carrying a cysteine to alanine substitution at position 184 (TACE Pro C184A). The mutant protein was expressed in E. coli and purified using the same protocol as for TACE Pro. TACE Pro C184A purified entirely as a monomer (data not shown) and had similar solution properties as its wild type counterpart. The fluorescence emission and CD spectra of TACE Pro C184A were basically indistinguishable from those of the wild type protein (Fig. 2). TACE Pro C184A also had similar equilibrium denaturation profiles to those of TACE Pro (Fig. 3).
Surprisingly, the introduction of this cysteine to alanine mutation had only a modest effect in the inhibitory potency of TACE Pro. The IC50 values obtained for TACE Pro C184A were 40 nM against the catalytic domain and higher than 2 µM against the mature ectodomain (catalytic plus disintegrin/cysteine-rich domains). This suggests that the cysteine residue cannot determine by itself the interaction between the pro and catalytic domains of TACE. Other residues within the cysteine switch region (PKVCGYLKVD190) do not appear to be critical for the pro-catalytic domain interaction either, because alanine substitutions at each individual position of the switch region, in the context of TACE Pro C184A, affected the inhibitory ability of TACE Pro modestly, within 2-fold of the IC50 observed with wild type TACE Pro (Table I). Additional sequence elements within the pro domain different from the cysteine switch region, yet to be identified, must mediate the pro-catalytic domain interaction.
|
0.45 eV in comparison with active TACE (catalytic domain alone). A shift in edge position is often an indication of structural modification at the metal site, such as different ligation (21). Specifically, this shift reflects a change in the total effective charge of the zinc ion in TACE, which is partially oxidized upon binding of the pro domain. The post-edge spectra exhibit slight modifications of the peak intensities around 9680 and 9737 eV, indicating mild conformational changes within the environment of the catalytic zinc ion of TACE.
|
|
2 (the sum of the residuals squared. It represents robustness of the fit) values. The zinc ligand distances derived from our EXAFS analysis for the pro enzyme are in agreement with the crystal structure of pro-MMP2 (22) and previous data of pro-MMP-2 EXAFS analysis (24).
The EXAFS curve fitting data analysis of active TACE shows that the zinc ion is tetrahedrally coordinated with three zinc-nitrogen (His) bond distances at 2.06 ± 0.01 Å, one zinc-nitrogen/oxygen bond distance at 1.89 ± 0.01 Å, three zinc-carbon bond distances at 2.85 ± 0.01 Å, and three zinc-carbon bond distances at 3.11 ± 0.01 Å. Attempts to fit the first coordination shell of the active enzyme with only three zinc-nitrogen/oxygen contributions or additional zinc-sulfur contributions resulted with unstable fits and high 2 values. The first coordination shell zinc-ligand bond distances of active TACE are in agreement with the x-ray crystal structure (23), showing three zinc-nitrogen contributions of the histidine residues chelating the catalytic zinc ion. Similarly, the catalytic zinc ion of MMP-2 is coordinated by three zinc-nitrogen (His) at 1.97 ± 0.02 Å and one zinc-nitrogen/oxygen (presumably zinc-oxygen contribution from water) at 2.01 ± 0.05 Å (24).
|
DISCUSSION |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
-lytic protease. For those enzymes, the pro domain is needed to catalyze protein folding by effectively decreasing the conformational energy barrier between the folded and unfolded states (folding under kinetic control) (14–17). On the other hand, the role of ADAM pro domains may be restricted to aid in the trafficking of these enzymes through the secretory pathway by acting as intramolecular chaperones without a direct role in catalysis of folding. In an analogous manner to several molecular chaperones, these pro domains may actually arrest the folding of the catalytic domain, instead of promoting it. Under this alternate interpretation, the final assembly of ADAM proteinases into their native, functional conformations occurs only after pro domain dissociation. At this point, there is no evidence to distinguish between these two possibilities for any ADAM. Pro domains have also been shown to be inhibitors of the catalytic domain on the basis that the enzyme only becomes fully active after removal of its pro domain (8, 12, 25). As mentioned above, ADAM 33 seems to be an exception to this rule, although definitive data to support this claim have not been provided yet (29). This pro domain removal can be either autocatalytic or mediated by a furin-like enzyme, depending on each individual ADAM family member (8, 12, 25–30). There is very limited structural information on the pro domains of ADAMs. Although extensive research has been done on the pro domains of MMPs, it is difficult to use that structural information to model the pro domains of ADAM, because of the low homology across the family within this region and the fact that the pro domains of ADAM are substantially larger than those of MMPs. To study the determinants of the interaction between the pro domain and the catalytic domain of TACE, we expressed TACE Pro in E. coli. We found TACE Pro to have a thermodynamically stable structure, according to both our fluorescence and circular dichroism spectroscopy data. Denaturation experiments using both spectroscopic probes showed that TACE Pro has reversible, two-state unfolding behavior under equilibrium conditions upon dilution in solutions containing the chemical denaturant Gdn-HCl. TACE Pro showed an unfolding transition at a relatively low Gdn-HCl concentration (1 M) suggesting that, although it can behave as an independent folding unit, its thermodynamic stabilization may depend on its association with the catalytic domain.
TACE Pro refolded in vitro proved to be a very potent inhibitor of its catalytic domain. It was previously reported that although a TACE pro-catalytic domain complex is basically inactive, the activity of the enzyme was recovered upon dilution of the protein complex (8). Recovery of activity by dilution occurs in the nanomolar range.2 The in vitro refolded TACE Pro inhibits the catalytic domain in this same range (IC50 = 70 nM). Therefore, this domain, after expression and purification in an isolated form, resembles the function of the naturally occurring pro domain. The inhibitory potency of TACE Pro was much weaker against the complete TACE ectodomain (catalytic plus disintegrin/cysteine-rich domains). This indicates that the disintegrin/cysteine-rich domain may play a role in displacing the pro domain of TACE from the catalytic domain upon processing by furin or a furin-like enzyme. We found that the disintegrin/cysteine-rich domain can remove the pro domain from the pro-catalytic complex in trans (Fig. 5). It is possible that part of the disintegrin/cysteine-rich domain of TACE may be positioned next to the substrate-binding cleft. Therefore, it may sterically hinder the interaction of the pro domain with this surface of the catalytic domain. In fact, Murphy and co-workers (31, 32) found that an unrelated proteinaceous inhibitor of TACE, tissue inhibitor of metalloproteinases-3 (TIMP-3), also exhibited decreased affinity against the ectodomain form of the enzyme relative to the isolated catalytic domain.
The pro domain of TACE contains a consensus cysteine switch box found in both the MMP and ADAM families. It has been proposed that the pro domain of metzincins exert their inhibitory role through ligation of the cysteinyl thiol to the zinc ion in the active site of these enzymes (9–11). Since the original proposal of the cysteine switch model (9), there have been conflicting reports on the importance of the cysteine and other residues within the pro domain in the folding and inhibitory roles of such domain in MMPs (9–11, 33). In the case of MMP3, at least two residues in addition to the cysteine within the switch box are important for maintaining the latency of the enzyme (34). Within the ADAM family, the role of this motif has been studied in ADAM 9, ADAM 10, and ADAM 12. The cysteine switch has been shown to be a key element in the pro-catalytic interaction (12, 13, 25). For ADAM 9 and ADAM 10, a Cys to Ala mutation in the switch box prevented the production of functional enzyme, probably because of protein misfolding. In the case of ADAM 12, the cysteine residue does not seem to be required for secretion, but a mutation to alanine renders the pro domain incapable of inhibiting the activity of the catalytic domain. In this report, we demonstrate that an intact cysteine switch is not required for inhibition of TACE by its pro domain. A TACE Pro variant carrying a Cys to Ala mutation at position 184 proved to have similar solution properties and inhibitory potency to those of its wild type counterpart. Other residues adjacent to this central cysteine residue in the switch box do not seem to be required for the pro-catalytic domain interaction either. This result is not in contradiction with a previous publication showing that 4-aminophenylmercuric acetate and octyl thioglucoside (two thiol-modifying reagents) are able to dissociate the pro-catalytic complex (8). As it has been reported previously for the MMPs, these reagents may disrupt the pro-catalytic domain interaction in a manner that does not involve thiol modification of the cysteine residue.
To address whether Cys184 actually ligated the catalytic zinc ion of TACE, we performed XAS analysis of both the free and Pro-bound catalytic domain species. XAS is a valuable spectroscopic technique for elucidating the local structure of a variety of metal-binding sites in metalloproteins (35). The successful application of XAS to study electronic and local structures of the catalytic zinc site in the related MMPs systems has been demonstrated (24). XAS spectra are divided into two regions: XANES and EXAFS. XANES provides information about the effective charge of the metal ion and its geometry. Complementary to this, analysis of the EXAFS region provides the local structure around the analyzed metal ion, which includes average bond distances, mean square variation in distance, metal coordination number, and ligand type. Interestingly, our EX-AFS results show that the binding of TACE Pro to the catalytic domain does involve ligation of the catalytic zinc ion in the enzyme via the sulfur ion of the conserved Cys184 amino acid residue. This indicates that the steady state pro-catalytic complex of TACE possesses a stable zinc-sulfur bond at the active site. However, our biochemical evidence shows that the binding of Cys184 of TACE Pro to the catalytic zinc site in TACE is energetically neutral. Differently from the MMPs, the zinc-sulfur (Cys) bond in TACE may be extremely susceptible to chemical attack by either adjacent polar residues within the active site or solvent molecules. Consistent with this hypothesis, the XANES features of active free catalytic domain and inactive pro-catalytic domain complex are different from the ones observed for MMP-2 and pro-MMP-2. This suggests that although the basic metal-ligand coordination is conserved, the chemical potential of the catalytic zinc site in TACE may be different relative to the MMPs. Such chemical diversity has been documented for TACE in comparative XAS studies with MMP-2 (36).
The above results pose an obvious question: if the cysteine switch of TACE is physically present in the zymogen form but is not needed for enzyme inhibition, what then is its function? Our own studies point at a role in preventing intracellular proteolysis of the TACE zymogen. An intact cysteine switch is not essential for the efficient biosynthesis and maturation of the TACE forms used in this study (catalytic domain and catalytic plus disintegrin/cysteine-rich domain). Mature, functional catalytic domain and TACE ectodomain were secreted from insect cells infected with baculoviral strains encoding the C184A variants of both enzyme forms. Furthermore, full-length TACE C184A was also biosynthesized and maturated similarly to its wild type counterpart in these cells. However, the only difference we could detect is very telling; significant intracellular degradation of the zymogen forms was observed when Cys184 was missing, suggesting that a cysteine switch in the "closed" position may be required for protecting TACE from intracellular degradation.3 This may explain both the relatively high degree of conservation of this motif throughout the ADAM family of proteinases and our direct confirmation of the Cys184 thiol coordination to the catalytic zinc ion by XAS methods (Fig. 6).
In summary, here we report on the biophysical properties and inhibitory activity of the pro domain of TACE. We demonstrate that this domain can act as an inhibitor of TACE in trans, being more potent against the catalytic domain than against the whole ectodomain. Our results question the application of a classical cysteine switch mechanism for maintenance of the zymogen state by TACE Pro. The importance of other regions outside the cysteine switch box of TACE in maintaining the latency of the enzyme is currently under investigation.
|
FOOTNOTES |
|---|
|| Supported by the Israeli Science Foundation Project 3771.
** To whom correspondence should be addressed: 242 Anatomy-Chemistry Bldg., 3620 Hamilton Walk, Philadelphia, PA 19104. Tel.: 215-898-7500; Fax: 215-573-4764; E-mail: mmilla{at}mail.med.upenn.edu.
1 The abbreviations used are: TACE, tumor necrosis factor--converting enzyme; TACE Pro, TACE pro domain; Gdn-HCl, guanidinium hydrochloride; XAS, X-ray absorption spectroscopy; XANES, x-ray absorption near edge spectra; EXAFS, extended x-ray absorption fine structure; MMP, matrix metalloproteinase.
2 M. A. Leesnitzer, unpublished observation.
3 J. D. Leonard, unpublished work.
|
ACKNOWLEDGMENTS |
|---|
|
REFERENCES |
|---|
|
TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J. Levine Molecular Mechanisms of Soluble Cytokine Receptor Generation J. Biol. Chem., May 23, 2008; 283(21): 14177 - 14181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Moss, M. Bomar, Q. Liu, H. Sage, P. Dempsey, P. M. Lenhart, P. A. Gillispie, A. Stoeck, D. Wildeboer, J. W. Bartsch, et al. The ADAM10 Prodomain Is a Specific Inhibitor of ADAM10 Proteolytic Activity and Inhibits Cellular Shedding Events J. Biol. Chem., December 7, 2007; 282(49): 35712 - 35721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-H. Koo, J.-M. Longpre, R. P. T. Somerville, J. P. Alexander, R. Leduc, and S. S. Apte Regulation of ADAMTS9 Secretion and Enzymatic Activity by Its Propeptide J. Biol. Chem., June 1, 2007; 282(22): 16146 - 16154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhu, W. Bergmeier, J. Wu, H. Jiang, T. J. Stalker, M. Cieslak, R. Fan, L. Boumsell, A. Kumanogoh, H. Kikutani, et al. Regulated surface expression and shedding support a dual role for semaphorin 4D in platelet responses to vascular injury PNAS, January 30, 2007; 104(5): 1621 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ohtsu, P. J. Dempsey, and S. Eguchi ADAMs as mediators of EGF receptor transactivation by G protein-coupled receptors Am J Physiol Cell Physiol, July 1, 2006; 291(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. M. Wewer, M. Morgelin, P. Holck, J. Jacobsen, M. C. Lydolph, A. H. Johnsen, M. Kveiborg, and R. Albrechtsen ADAM12 Is a Four-leafed Clover: THE EXCISED PRODOMAIN REMAINS BOUND TO THE MATURE ENZYME J. Biol. Chem., April 7, 2006; 281(14): 9418 - 9422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wei, M. Kashiwagi, S. Kota, Z. Xie, H. Nagase, and K. Brew Reactive Site Mutations in Tissue Inhibitor of Metalloproteinase-3 Disrupt Inhibition of Matrix Metalloproteinases but Not Tumor Necrosis Factor-{alpha}-converting Enzyme J. Biol. Chem., September 23, 2005; 280(38): 32877 - 32882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Gomez, S. H. Sokol, A. B. Muir, G. Soong, J. Bastien, and A. S. Prince Bacterial Induction of TNF-{alpha} Converting Enzyme Expression and IL-6 Receptor {alpha} Shedding Regulates Airway Inflammatory Signaling J. Immunol., August 1, 2005; 175(3): 1930 - 1936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
, native TACE Pro C184A; 
, denatured TACE Pro; 
], (deg·cm2/dmol) x 10-3.
