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J. Biol. Chem., Vol. 280, Issue 32, 29392, August 12, 2005

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Correction

Stable gene silencing in human monocytic cell lines using lentiviral-delivered small interference RNA. Silencing of the p110 isoform of phosphoinositide 3-kinase reveals differential regulation of adherence induced by 1,25-dihydroxycholecalciferol and bacterial lipopolysaccharide. Vol. 279 (2004) 9379-9388

Page 9382, Table I: The sequences of the oligonucleotides were not printed correctly. The last underlined GATC sequence on the first oligo should be located 5' of the second oligo. Similarly, the last underlined GATC sequence at the end of the third oligo should be at the 5' end of the fourth oligo. The corrected Table I is shown below. Although this does not affect the outcome of the published article, it may be impossible for other laboratories to reproduce the results if not corrected.

View this table: [in this window] [in a new window]   TABLE I Short hairpin RNA encoding sequences targeting human p110 mRNA Hairpin sequences with the HindIII site are underlined and italicized. For each construct, the two strands of DNA were annealed and ligated into the pSHAG-1 vector. The underlined sequences at the 5' and 3' ends are for directional cloning into pSHAG-1, which was cut with BseRI and BamH1. See "Experimental Procedures" for details.

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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lee, J. S. Articles by Reiner, N. E. Search for Related Content PubMed Articles by Lee, J. S. Articles by Reiner, N. E. Social Bookmarking                      What's this?