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J. Biol. Chem., Vol. 280, Issue 50, 41222-41228, December 16, 2005
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From the Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892
Received for publication, August 31, 2005 , and in revised form, October 3, 2005.
| ABSTRACT |
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itself and TH/bZIP (TH-responsive basic leucine zipper transcription factor). By using an antibody that recognizes both TR
and TR
, we found that TR binding to the TR
promoter is indeed constitutive. Most surprisingly, TR binding to the TH/bZIP promoter increases dramatically after TH treatment of premetamorphic tadpoles and during metamorphosis. By using an antibody specific to TR
,TR
binding increases at both promoters in response to TH. In vitro biochemical studies showed that TRs bind TH/bZIP TRE with 4-fold lower affinity than to TR
TRE. Our data show that only high affinity TR
TRE is occupied by limiting levels of TR during premetamorphosis and that lower affinity TH/bZIP TRE becomes occupied only when overall the TR expression is higher during metamorphosis. These data provide the first in vivo evidence to suggest that one mechanism for tissue- and gene-specific regulation of TR target gene expression is through tissue and developmental stage-dependent regulation of TR levels, likely a critical mechanism for coordinating development in different organs during postembryonic development. | INTRODUCTION |
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and TR
, that regulate gene expression by binding thyroid hormone (TH)-response elements (TREs) of TH-inducible genes and recruiting cofactors (1). In the absence of TH, TRs recruit corepressors, including N-CoR, SMRT, TBL1/TBLR1, HDAC3, and GPS2 (27). Corepressor binding is associated with deacetylated histones in the TRE region and gene repression. In the presence of TH, coactivators, such as SRC, p300, TRAP, and Mediator complexes, replace corepressors (811). Coactivator binding promotes transcription by acetylating histones and interacting with the basal transcriptional machinery.
The above knowledge based on in vitro studies complements extensive studies on the developmental role of TRs in frogs and TR knock-out mice. TR is important for postembryonic development of many organs (12). The destruction of larval organs and the formation of adult organs during frog metamorphosis is totally dependent upon TH (13, 14). Transgenic overexpression of mutant TRs and cofactors showed gene activation by TR is necessary and sufficient to initiate TH-dependent developmental transitions in frogs (15, 16). In knock-out mice lacking TRs, developmental defects are evident in brain, heart, and intestine, among other organs (17). Similar observations on the importance of TR in development have been noted in all vertebrates studied, including humans (18), fish (19), and chickens (20). Most interestingly, the phenotype of TR knock-out mice is dramatically different from mice lacking TH (17). Therefore, the study of the molecular mechanisms of TR in gene regulation in vivo is important for understanding the developmental actions of TR.
To bridge the gap between our knowledge of the developmental roles in vivo and the molecular mechanisms of TR action in vitro, we developed the dual function model for the role of TR during development (21, 22). Frog metamorphosis is a valuable model for studying hormonal control of postembryonic development because premetamorphic tadpoles naturally lack TH, and the large size of tadpoles allows direct study of the molecular mechanisms of gene regulation during development in vivo. In frogs, TR
is expressed throughout larval development, well before TH is synthesized and secreted into the blood, and both TR
and TR
are expressed during metamorphic climax, characterized by extensive organ remodeling and high levels of TH (14). In the dual function model, during premetamorphosis TR
binds corepressors to deacetylate histones and down-regulate TH-response genes, while during metamorphic climax, TR
and TR
activate those same genes by acetylating histones via binding coactivators in the presence of TH. In vivo support for this model comes from chromatin immunoprecipitation studies on TH-responsive promoters. Corepressor binding has been detected at TREs in premetamorphic tadpoles, and this binding is associated with low levels of acetylated histones (2325). Conversely, coactivator binding with high levels of acetylated histones has been found during metamorphic climax (4, 26, 27).
A critical parameter for the dual function model is TR binding to TH-inducible genes in vivo. Even though ChIP assays suggest that TRs bind during both premetamorphosis and climax when TH is absent and present, respectively, developmental changes in TR expression hinted that levels of TR binding to TH-response promoters might vary dramatically during development. Here we used quantitative PCR (qPCR) to quantify TR binding to two different promoters during development, and we identified a contributing factor to differences we observed for TR binding between the promoters. These results expand our understanding of tissue- and gene-dependent roles of TR during development.
| MATERIALS AND METHODS |
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We used the following two rabbit antisera against TR: 1) anti-TR(PB) made by injecting full-length TR
but recognizes both TR
and TR
(29), and 2) anti-TR
made by coinjecting two synthetic peptides, REKRRKDEIQKSLVQKPEPT (amino acids 104123 of TR
synthesized on 8-MAP) and DRPGLASVERIEK (amino acids 290302 of TR
conjugated to keyhole limpet hemocyanin). Anti-ID14 antiserum made in rabbits immunized against the peptide ETKCRCNMDGDVE conjugated to 8-MAP (Invitrogen) was used as a negative control. ID14 is a novel extracellular protein expressed by intestinal epithelial cells (30).
Chromatin ImmunoprecipitationChromatin was isolated from tadpole tails or intestines flushed with 0.6x phosphate-buffered saline. Organs were placed in 1 ml of nuclei extraction buffer (0.5% Triton X-100, 10 mM Tris-HCl, pH 7.5, 3 mM CaCl2, 0.25 M sucrose, with protease inhibitor tablet (Roche Applied Science, Complete, Mini, EDTA-free), 0.1 mM dithiothreitol, and 0.2 mM phenylmethylsulfonyl fluoride) in Dounce homogenizers on ice and crushed with 1015 strokes using pestle A (Kontes). The homogenate was fixed in 1% formaldehyde with rotation at room temperature for 20 min, and the fixation was stopped with 0.1 M Tris-HCl, pH 9.5. The homogenate was centrifuged at 2000 x g at 4 °C for 2 min, and the pellet was resuspended in 1 ml of nuclei extraction buffer and re-homogenized in Dounce with 510 strokes using pestle A for tails and pestle B for intestines. Then the homogenate was filtered through a Falcon 100-µm cell strainer and centrifuged at 2000 x g at 4 °C for 2 min. The pellet was resuspended in 200300 µl of SDS lysis buffer (Upstate Cell Signaling Solutions) on ice, sonicated to an average length of 800 bp, and centrifuged at 16,000 x g for 10 min at 4 °C. The chromatin in the supernatant was quantitated and frozen in aliquots at -80 °C.
For immunoprecipitation as reported previously (25), the DNA concentration of the chromatin was adjusted to 100 ng/µl using the SDS lysis buffer, and then diluted to 10 ng/µl with ChIP dilution buffer (Upstate Cell Signaling Solutions). After preclearing with salmon sperm DNA/protein A-agarose (Upstate Cell Signaling Solutions), input samples were taken, and 500 µl of each chromatin sample was added to tubes with anti-TR(PB), anti-TR
, or anti-ID14 antibodies and salmon sperm DNA/protein A-agarose beads and incubated with rotation from 4 h to overnight at 4 °C. After incubation, the beads were washed with 1 ml of ChIP Buffer I (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 50 mM HEPES, pH 7.5, 150 mM NaCl), Buffer II (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 50 mM HEPES, pH 7.5, 500 mM NaCl), Buffer III (0.25 M LiCl, 0.5% Nonidet P-40, 0.5% sodium deoxycholate, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0), and TE (10 mM Tris-HCl, pH 8.1, 1 mM EDTA, pH 8.0) in succession. After the last wash, 100 µl of elution buffer (0.5% SDS, 0.1 M NaHCO3 (Sigma), 25 µg/ml proteinase K (Roche Applied Science)) was added to the samples and input controls and rotated at 65 °C for 6 h to overnight. The ChIP DNA was purified using the QIA-quick PCR purification kit (Qiagen) and eluted with 40 µl of EB buffer (Qiagen, 10 mM Tris-HCl, pH 8.5). Analysis of ChIP DNA was done as described previously for radioactive PCR followed by gel electrophoresis and autoradiography (25) and quantitative PCR (16). Analysis of variance and post hoc tests of quantitative PCR results were carried out using Statview statistical software (Abacus Concepts, Berkeley, CA).
Gel Mobility Shift AssayReceptor mRNA was made for TR
A, TR
AII, or RXR
contained in pSP64(poly(A)) (29, 31) using mMessage mMachine (Ambion). Large batches of oocyte extracts for TR
, TR
, and RXR
were prepared by injecting 3050 oocytes with 23 nl of 330 ng/µl receptor mRNA. After overnight incubation, surviving oocytes were homogenized in 20 µl of binding buffer per oocyte (20 mM HEPES, pH 7.5, 60 mM KCl, 5 mM MgCl2, 5 mM dithiothreitol, 10% glycerol, 0.1% Nonidet P-40, and 1 mM phenylmethylsulfonyl fluoride). Homogenate was centrifuged in an Eppendorf microcentrifuge at top speed at 4 °C for 10 min. Supernatant was collected and recentrifuged to remove lipids and debris. Aliquots were frozen at -80 °C. To ensure similar amounts of TR
and TR
in the extracts, a preliminary experiment was done by injecting TR
and TR
mRNA and metabolically labeling the proteins with [35S]methionine. Oocyte extracts were then run on a gel and exposed to film to determine the amount of mRNA needed to produce equal amounts of TR
and TR
by taking into account the number of methionines in each receptor.
Probes for the gel mobility shift assay spanned the single TRE in TR
(31) and the two TREs in TH/bZIP (32) and had 4-bp overhangs on both 5' and 3' ends. The sequences were 5'-CGTCCTCCCTAGGCAGGTCATTTCAGGACAGCCCAGCGCCC and 5'-ACCAGGGCGCTGGGCTGTCCTGAAATGACCTGCCTAGGGAG for TR
and 5'-ACTAGGGTTAAGTAAGGTGAATGCTCAGCCTCATTTGAACT and 5'-ACAGAGTTCAAATGAGGCTGAGCATTCACCTTACTTAACCC for TH/bZIP. Probes were annealed by adding 5 µl of 5 pmol/µl of each strand to Buffer III (New England Biolabs) in a 1.7-ml Eppendorf tube, putting the tubes in a beaker of boiling water, and allowing them to cool to room temperature. After ethanol precipitation, the pellet was dissolved in 12 µl of TE buffer, and the probe was labeled with T4 polynucleotide kinase in the forward reaction buffer in a 25-µl reaction (Invitrogen). After stopping the reaction and increasing the volume to 50 µl, unincorporated nucleotides were removed using a G-50 column (Amersham Biosciences) so that the final probe concentration was 0.1 pmol/µl.
Gel mobility shift assays were done based on procedures described previously (29, 31). Oocyte extracts (12 µl) from oocytes injected with RXR
and oocytes injected with either TR
or TR
were mixed and incubated on ice for 10 min. The binding buffer (enough for a final reaction volume of 15 µl) and 1 µl of 2 µg/µl of sonicated poly(dI-dC) (Amersham Biosciences) in binding buffer was then added and incubated for 20 min at room temperature. 1 µl of 0.1 pmol/µl labeled probe and, as indicated, 315x unlabeled probe was then added and incubated for another 20 min. Gel mobility shift samples were run on a 6% PAGE in 0.5x TBE buffer (Invitrogen) at 250 V for 15 min. Gels were dried and exposed to film 15 min to overnight. Scatchard plot analysis was done using the public domain NIH Image program (developed at National Institutes of Health and available on the internet at rsb.info.nih.gov/nih-image) to determine the total counts bound and total counts free and then graphing bound versus bound/free in Statview (Abacus Concepts) to calculate the slope.
In Vitro Transcription and Western BlottingThe pSP64(poly(A)) vector (Promega) containing TR
A or TR
AII (29) was linearized with EcoRI and in vitro transcribed and translated following the manufacturer's protocol (TNT Quick Coupled transcription/translation system, Promega). Standard protocols for SDS-PAGE and protein transfer were performed, followed by Western blotting using anti-TR(PB) and anti-TR
(38) antibodies and ECL-plus chemiluminescent detection (Amersham Biosciences).
| RESULTS |
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and TH/bZIP, in the intestine and tail with anti-TR(PB) antibody (Fig. 1). TR binding remained constant at the TR
promoter in the presence or absence of T3 in both the intestine and tail (Fig. 1, top panels), and this constitutive binding was found at the TH/bZIP promoter in the intestine as well (Fig. 1, lower panels). However, an increase in TR binding was detected in the presence of T3 in the tail (Fig. 1, lower panels).
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exon 5. The control DNA region, exon 5 of the TR
gene, does not contain a TRE and is at least 20 kb away from the promoter where the TRE is located (33). This controls for sufficient sonication of the chromatin in the assay. Thus, the very low amounts of precipitated exon 5 DNA, expressed as % input, for both antibodies, anti-TR(PB) and anti-ID14, indicate that the chromatin was sonicated sufficiently to avoid chromatin fragments containing both the TRE and exon 5. We suggest that this level of % input at exon 5 represents the background for the assay. We also found background levels of % input at the TH/bZIP and TR
TREs with the anti-ID14 antibody, indicating the washing steps after immunoprecipitation effectively removed nonspecific interactions.
Consistent with the above analysis, TR binding to the TH/bZIP TRE was dramatically and statistically significantly increased in the presence T3 in both the tail (F3,10 = 7.9, p < 0.005 (This F-statistic has 3 and 10 degrees of freedom reflecting treatment levels and sample sizes.)) and intestine (F3,10 = 9.1, p < 0.003), where the maximal levels of increase after T3 treatment were about 10- and 7-fold, respectively (Fig. 2, A and B). Scheffe's post hoc tests revealed no differences in the immunoprecipitated TRE region as % input among T3-treated samples. These same ChIP DNA samples were used to quantitate TR
TRE immunoprecipitation, where less dramatic increases were observed, i.e. the immunoprecipitated TRE region as % input changed less than 2-fold in the tail and 24-fold in the intestine (Fig. 2, A and B) (TABLE ONE). This smaller change at the TR
TRE was not detectable using conventional PCR analysis, and indeed, because of the differences in values when using chromatin isolated from multiple animals with different days of T3 treatment, the changes caused by T3 treatment in the tail were not statistically significant (F3,10 = 3.3, p < 0.068), although the increase in the intestine was significant (F3,10 = 16.3, p < 0.0004).
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TRE, no significant differences in TR binding across development were observed in the tail (F3,22 = 1.5, p < 0.23) or the intestine (post hoc tests showed no pairwise significant difference, although TR binding differences were significant overall, F3,14 = 4.1, p < 0.028).
TR
and TR
Have a Higher Affinity for TR
TRE Compared with the TH/bZIP TREA generality based on the above data is that the level of TR binding to the TH/bZIP TRE in tails and intestines of untreated, premetamorphic tadpoles was at or marginally above background levels, based on signal from exon 5 and control antibody. In addition, the TR binding at the TR
TRE in premetamorphosis was much higher above background compared with the TH/bZIP TRE, especially in the tail. In the presence of T3, an increase in TR binding was greater at the TH/bZIP TRE than at the TR
TRE. These differences between TR
and TH/bZIP TREs motivated experiments to determine the underlying basis for the differences between promoters. By using gel mobility shift assays, we tested several possibilities that may explain the differences as follows. 1) The T3-induced conformational change in TR per se may increase its affinity for the TH/bZIP promoter. 2) TR
, which is the predominant TR before T3 treatment, may have a lower affinity than TR
for the TH/bZIP TRE. 3) TRs may have different affinities for TR
versus TH/bZIP TREs.
For the gel mobility shift assays, we isolated frog oocyte cytoplasm containing TR
,TR
, or RXR after injection of corresponding mRNAs. We adjusted mRNA concentration for production of the oocyte cytoplasm based on [35S]methionine incorporation into the TRs after a pilot mRNA injection so that the isolated cytoplasms had equal amounts of the two TRs (data not shown), and we confirmed this result by Western blot using the same antibody as in the above ChIP experiments, anti-TR(PB), that recognizes both TR
and TR
(Fig. 3A). Next, we showed this cytoplasm was competent in the gel mobility shift assays, where a shift was seen when TR, either
or
, and RXR were coincubated in the binding reaction and not when only one receptor or no receptors were included (Fig. 3B).
To address the simple possibility that there is some difference in the ability to bind the TH/bZIP TRE versus TR
TRE in the presence or absence of T3, we carried out a gel mobility shift assay that included T3 (Fig. 3C). The results showed equal binding by both TRs to both TREs in the presence or absence of T3. The slight difference in migration in the presence of T3 is likely due to a conformational change in the TRs induced by T3, causing different migration on the nondenaturing gels (36).
The other potential explanations for the low binding in TH/bZIP in the absence of T3 may relate to binding affinity differences between the two TR isoforms or between TREs. First, we performed a TRE competition experiment where TR binding to radiolabeled TREs was competed with unlabeled TREs (Fig. 4). By using radiolabeled TR
TRE, we incubated mixtures of TR
and RXR or TR
and RXR with increasing amounts of cold TR
TRE or TH/bZIP TRE (Fig. 4A). For both TR
and TR
, cold TR
TRE was a better competitor than cold TH/bZIP TRE for TR binding as shown by the greater reduction of bound radiolabeled TR
TRE in the presence of cold TR
TRE (Fig. 4A, compare lanes 24 with 57 and lanes 911 with 1214). Similar results were obtained with the reciprocal experiment using radiolabeled TH/bZIP TRE (Fig. 4B), showing that cold TR
TRE again competed more efficiently than TH/bZIP TRE (compare lanes 24 with 57 and lanes 911 with 1214).
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TRE more strongly than the TH/bZIP TRE, TR
may not bind the TREs as strongly compared with TR
. In premetamorphic tadpoles, TR
is predominant, and TR
expression is very low, whereas TR
expression is up-regulated in the presence of T3 (37). Thus, the low TR binding to TH/bZIP may be due to a potentially low TR
binding affinity for TH/bZIP TRE and low TR
protein levels in premetamorphosis. To examine potential differences in binding to the two TREs between the TR isoforms, we performed a Scatchard plot analysis (Fig. 5). We used either radiolabeled TR
or TH/bZIP TREs in decreasing amounts in the binding reactions with a constant amount of TR
or TR
in a gel mobility shift assay (Fig. 5, A and B). These radiographs were used to determine the total amount of bound and free TRE from each binding reaction for the Scatchard plot analysis (Fig. 5C). The results corroborate data from the above competition experiments showing that for each TR the binding affinity is higher for TR
TRE than TH/bZIP TRE with the Kd (dissociation constant) for TR
TRE 4-fold lower than the Kd for the TH/bZIP TRE. In addition, both TRs had identical Kd values for either TR
TRE or TH/bZIP TRE, indicating identical affinity for each TRE by TR
and TR
.
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Expression by TH Leads to High Occupancy of TR
at TREsThe increase in TR binding at TREs after T3 treatment suggests that TR levels are not sufficient to saturate TREs, at least the weaker TH/bZIP TRE in premetamorphic tadpoles. As T3 treatment preferentially induces TR
expression (37), we would predict that TR
binding at TREs would increase more dramatically than total TR binding to the TREs. To examine this hypothesis, we performed ChIP assay using a TR
-specific antibody on tail and intestine during development. First, we generated a TR
-specific antibody by immunizing a rabbit with two TR
-specific peptides (see "Materials and Methods"). Western blot analysis of in vitro translated TR
and TR
with this polyclonal antibody, anti-TR
, showed that it is specific for TR
(Fig. 6A), even though similar amounts of TR
and TR
were present as shown by the anti-TR(PB) antibody used above (Fig. 6B). When the ChIP assay was carried out with anti-TR
antibody comparing control and T3-treated tadpoles, TR
binding increased by about 10-fold at the TH/bZIP TRE and 3-fold at the TR
TRE in both tails (F3,10 = 16.8, p < 0.0003 for TH/bZIP and F3,10 = 5.7, p < 0.015 for TR
) and intestines (F3,8 = 7.8, p < 0.009 for TH/bZIP and F3,8 = 2.6, p < 0.12 for TR
) (Fig. 7, A and B). These increases were similar or slightly higher than those observed with the anti-TR(PB) antibody. During natural metamorphosis, TR
binding to the TH/bZIP TRE gradually increased to 20- and 10-fold in the tail and intestines, respectively, from premetamorphosis to metamorphic climax (Fig. 7, C and D). At the TR
TRE during natural metamorphosis, the increases were 5- and 2-fold in the tail and intestines, respectively. The level of TR
binding was at background control antibody levels for the TH/bZIP TRE for both organs in premetamorphic tadpoles and was at or marginally above background levels for the TR
TRE and increased much more during metamorphosis than that observed for anti-TR(PB) antibody (TABLE ONE). These results are consistent with the relative changes in TR
and TR
expression during natural and T3-induced metamorphosis (see "Discussion").
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| DISCUSSION |
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expression in premetamorphic tadpoles (37, 38). At the same time, we found limited change in TR binding to the TR
TRE in response to TH. The accuracy of these results was initially in doubt because it was based on conventional PCR and autoradiography, techniques of dubious quantitative value. Therefore, we carried out qPCR and confirmed promoter-specific, differential recruitment of TR to the two TREs. Next, we used gel mobility shift assays to investigate the underlying cause for the differences in TR binding between the two promoters in vivo. Our data showed that TR
and TR
bind to the TREs with identical affinity and that the TH/bZIP TRE has a 4-fold weaker affinity than TR
TRE for either receptor, a result consistent with a sequence comparison of the TR
and TH/bZIP TREs with a consensus TRE (Fig. 8) (39). The TR
TRE has a single nucleotide different from the consensus, whereas the two TH/bZIP TREs have 3 or 4 nucleotide differences each. Most interestingly, even though there are two TREs in the TH/bZIP promoter, only a single TR/RXR heterodimer is bound under gel mobility shift conditions (our data and see Ref. 40).
The combination of results from the ChIP and gel mobility shift assays suggests the following hypothesis explaining the differences in TR binding in vivo for the two promoters. TH/bZIP TRE occupancy by TRs is low in premetamorphosis because of limiting protein levels of TR, which allows the binding to the 4-fold higher affinity TR
TRE-binding sites. Then, in T3-induced or natural metamorphosis, even the lower affinity TH/bZIP TRE-binding sites would be occupied because of the T3-induced expression of TR
and TR
. Furthermore, the relatively little change in TR binding at the TR
TRE implies that the TR expression levels in premetamorphosis are able to saturate these high affinity TRE-binding sites so that even during metamorphosis with higher levels of TR little binding occurs.
We examined a corollary of the above hypothesis that the TR
isoform binding would be low at both promoters in premetamorphosis when TR
expression was low and would increase at both promoters in the presence of T3 after enough time had occurred for TR
synthesis. We predicted this increase would be greater than the increase observed with the anti-TR(PB) antibody that recognizes both TR isoforms. Indeed, the increase for TR
binding during natural metamorphosis was much greater than that seen for the other antibody at both promoters. Although the increase in TR
binding compared with total TR binding was less dramatic during T3 treatment, this result was consistent with the relative expression of TR
and TR
during T3 treatment. During natural metamorphosis, TR
mRNA expression increases only 23-fold in both the intestine and tail, whereas TR
mRNA increases by over 10-fold (4143). This leads to a much higher TR
to TR
ratio at climax than in premetamorphosis, thereby leading to a higher increase in TR
binding to TREs than in total TR binding. In contrast, during T3 treatment both TR
and TR
increase dramatically at least in the tail (43); thus even though overall TR binding increased at both promoters, the relative proportions of TR
might not increase significantly, as we observed.
The results of this study have strong implications for the role of TR in development. The dual function model for the role of TR states that TH-responsive promoters are repressed during premetamorphosis and activated during metamorphosis because of the critical role of ligand for TR function (21). No exceptions to this model have been identified so far regarding the up-regulation of direct response genes by T3 during metamorphosis (22). However, our ChIP results show that not all T3-response genes are bound by TR during premetamorphosis, indicating that TR does not regulate these genes before metamorphosis. Rather, the lack or low levels of expression of some TH-response genes before metamorphosis, such as TH/bZIP (41, 43), must be through TR-independent mechanisms via other transcription factors or formation of a repressive chromatin structure. Thus, our data indicate that the application of the dual function model during premetamorphosis cannot be universally applied to all T3-response genes and needs to incorporate TRE affinity for TRs and changing levels of TR during development. During premetamorphosis, the dual function model applies only to those genes with high affinity TREs. As receptor levels increase continuously during development (37), more and more promoters containing weaker TREs come under positive regulatory control of TR and TH.
Another hypothesized mechanism for TR function in development is its autoinduction. Previous studies in frogs revealed that TH regulates its own receptors, and this autoregulation is thought to be important for the developmental role of TH because it is correlated with metamorphic progression. However, because of the lack of knock-out technology in frogs, this hypothesis has not been directly tested. Our results here and the kinetics of TR
and TH/bZIP mRNA expression indirectly implicate a critical role of autoregulation. The kinetics of TR
and TH/bZIP mRNA expression are different after T3 treatment, where transcription of the TR
gene is up-regulated to maximal levels well before that from the TH/bZIP gene, which exhibits a second wave of increase in mRNA levels after T3 treatment (43). The initial rate of increase of TH/bZIP transcript may be limited by relatively low levels of TR binding at the promoter because of the lower affinity TRE, whereas after the start of T3 treatment when sufficient TR synthesis has occurred, the TR binding to the TH/bZIP TRE may be increased enough to promote a boost in mRNA production. Lack of autoregulation would likely result in the inability of TR to induce genes important for metamorphosis, such as TH/bZIP, due to results from our ChIP showing low TR binding to TH/bZIP TRE before metamorphosis.
Amphibian metamorphosis involves complex coordination of different transformations at different developmental stages in various organs. Different TH-response genes may play roles at different stages of metamorphosis in different organs/tissues or even within a single tissue, and their expression levels would need to be controlled accordingly. For example, limb development occurs much earlier, at the onset of metamorphosis, than tail resorption, which is the last process to complete. One mechanism to control such developmental timing is to have tissue-specific TR expression levels for controlling tissue sensitivity, i.e. the ability to activate T3-response genes (44). Specifically, the tissues first to transform during metamorphosis, such as the hind limb, would have sufficiently high levels of TR to respond to T3 early during metamorphosis compared with those transforming later, such as the tail, which is known to be the case (44). In addition, different direct T3-response genes may function at different time points during transformation of a given tissue. For example, TR
genes may function earlier, and its activation may be needed for the optimal expression of other T3-response genes, such as TH/bZip, which may function later. By having TREs with different affinities at different genes, gene-specific temporal regulation of T3-response genes can be achieved by regulating TR levels at least in part through autoinduction of the TR
genes. Without such tissue- and gene-specific control of gene expression, some genes, such as TH/bZip, may be precociously activated. This would in turn lead to uncoordinated tissue transformations, thereby resulting in developmental defects.
| FOOTNOTES |
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1 To whom correspondence should be addressed: Bldg. 18 T, Rm. 106, Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, MD 20892. Tel.: 301-402-1004; Fax: 301-402-1323; E-mail: Shi{at}helix.nih.gov.
2 The abbreviations used are: TR, TH receptor; TH, thyroid hormone; TRE, TH response elements; ChIP, chromatin immunoprecipitation; T3, triiodothyronine; qPCR, quantitative PCR; bZIP, basic leucine zipper transcription factor; RXR, retinoid X receptor. ![]()
| REFERENCES |
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