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J. Biol. Chem., Vol. 281, Issue 15, 10035-10041, April 14, 2006
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1
From the
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 and
Antibiotic Research Institute, Sandoz GmbH, Brunner Strasse, A-1235 Vienna, Austria
Received for publication, August 10, 2005 , and in revised form, February 3, 2006.
| ABSTRACT |
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-lactam antibiotics, an event that leads to bacterial death. The gene product of mecA from MRSA is a penicillin-binding protein (PBP) designated PBP 2a. PBP 2a is refractory to the action of all commercially available
-lactam antibiotics. Furthermore, PBP 2a is capable of taking over the functions of the other PBPs of S. aureus in the face of the challenge by
-lactam antibiotics. Three cephalosporins (compounds 13) have been studied herein, which show antibacterial activities against MRSA, including the clinically important vancomycin-resistant strains. These cephalosporins exhibit substantially smaller dissociation constants for the preacylation complex compared with the case of typical cephalosporins, but their pseudo-second-order rate constants for encounter with PBP 2a (k2/Ks) are not very large (
200 M1 s1). It is documented herein that these cephalosporins facilitate a conformational change in PBP 2a, a process that is enhanced in the presence of a synthetic surrogate for cell wall, resulting in increases in the k2/Ks parameter and in more facile enzyme inhibition. These findings argue that the novel cephalosporins are able to co-opt interactions between PBP 2a and the cell wall in gaining access to the active site in the inhibition process, a set of events that leads to effective inhibition of PBP 2a and the attendant killing of the MRSA strains. | INTRODUCTION |
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-lactams in the clinic. In the 1940s, resistance to first generation penicillins emerged from a profusion of strains containing a class A
-lactamase. In response to the
-lactamase challenge, a second generation of penicillins that included methicillin was introduced in 1959. By 1961, a strain of S. aureus emerged first in the United Kingdom and subsequently in other parts of the world, which was resistant to methicillin and other
-lactams. This strain became known as methicillin-resistant S. aureus (MRSA).2 The recent emergence of variants of MRSA that are resistant to glycopeptide antibiotics, such as vancomycin, due to the acquisition of the vanA gene from Enterococci, has created a new and disconcerting chapter in the evolution of this organism. MRSA is one of the most serious infectious disease agents globally. The need for novel antibiotics to treat this organism is genuine.
The basis for MRSA resistance to
-lactam antibiotics is complex (15). Nonetheless, a primary factor is the acquisition of the gene mecA, which encodes a penicillin-binding protein (PBP) designated as PBP 2a. Whereas
-lactam antibiotics can inhibit their target PBPs in penicillin-sensitive S. aureus strains, a process that impairs the final steps of cell wall biosynthesis and leads to bacterial death, PBP 2a is refractory to inhibition by all commercially available
-lactam antibiotics (58). Hence, whereas
-lactam antibiotics inhibit the regular S. aureus PBPs, the
-lactam-insensitive PBP 2a takes over their biosynthetic functions. Hence, MRSA acquires a survival advantage to the challenge by
-lactam antibiotics. The basis for PBP 2a resistance to inhibition is complex. The rate constant for acylation of the active site serine by the
-lactam is attenuated, and the dissociation constant for the preacylation
-lactam-enzyme complex is less favorable (6, 9). An additional reason for PBP 2a
-lactam resistance would appear to be the lack of access of the
-lactam to its active site. The active site of PBP 2a is normally closed and must open to bind to the bacterial cell wall, its substrate (10, 11). We recently documented that in concert with interactions with cell wall fragments outside the active site, the active site becomes more accessible by a conformational change (10).
The clinical difficulties in treatment of MRSA have prompted the pharmaceutical industry to search for new
-lactams active against the PBP 2a of MRSA (1214). We report herein our analyses with three novel cephalosporins, 13 (Fig. 1), discovered at the Sandoz Antibiotic Research Institute of Vienna. Cephalosporin 1 is currently undergoing clinical trials for treatment of MRSA infections. These cephalosporins are found to have significantly smaller dissociation constants for the preacylation complex with PBP 2a compared with other
-lactam antibiotics, as will be discussed. However, the rate constants for the PBP 2a acylation by these three are similar to those for other
-lactams. Moreover, the rate constants for PBP 2a acylation by these cephalosporins are enhanced considerably in the presence of an analogue of a fragment of bacterial cell wall (compound 4). This suggests that these cephalosporins might be co-opting interactions of the cell wall with PBP 2a in vivo to gain access to the active site of this enzyme in the course of covalent inhibition that leads to bacterial death.
| EXPERIMENTAL PROCEDURES |
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500 =+15,900 cm1 M1). The experiments were carried out in 25 mM Hepes, 1 M NaCl (pH 7.0) buffer. Nitrocefin (120 µM) was used as the reporter molecule to determine the apparent first-order rate constants for acylation by the nonchromogenic (or poorly chromogenic) cephalosporins 13 at varying concentrations (10120 µM) in the presence and absence of compound 4 (0.52 mM) by the methods of Graves-Woodward et al. (15). Compounds 13 were dissolved in 25 mM Hepes, 1 M NaCl (pH 7.0) buffer. UV-spectroscopic analysis of the compounds in the range of 200350 nm at room temperature for 4 days indicated no change, consistent with the stability of the antibiotics within this duration. The deacylation rate constants (k3) were determined using BOCILLIN FL as a reporter molecule. A typical reaction mixture (60 µl) contained 15 µM PBP 2a and cephalosporin 1, 2, or 3 at a concentration 2-fold higher than its Kd value. The mixture was incubated at room temperature for 45 min in 25 mM Hepes, 1 M NaCl (pH 7.0) buffer. The excess cephalosporin was removed by passing the mixture through a Micro Bio-Spin®6 column (Bio-Rad). Deacylation rate constants for cephalosporins 13 were determined both in the presence and absence of the cell wall surrogate compound 4. In the experiments in the presence of compound 4, it (1.5 mM) was added to the resultant mixture immediately following the removal of excess antibiotic by passing the mixture through a Micro Bio-Spin®6 column (Bio-Rad). An aliquot (3 µl) of the mixture was diluted 5-fold with the same buffer and incubated at room temperature for different time intervals. The amount of free protein, liberated from the acyl-enzyme species, was assayed by the addition of BOCILLIN FL to afford a final concentration of 40 µM and incubated for an additional 20 min at room temperature. SDS sample buffer (15 µl) was added to the reaction mixture, which was then boiled for 3 min. The samples (30 µl in total) were loaded onto a 10% SDS-polyacrylamide gel, which was developed and then scanned using a Storm840® Fluorimager.
Circular DichroismThe CD spectra of PBP 2a (3 µM in 25 mM Hepes, 1 M NaCl, pH 7.0) were recorded on a stopped-flow circular dichroism spectrometer (AVIV INSTRUMENTS INC-202 SF) with a 2-mm path length in the absence and presence of a 6 µM concentration of each of the cephalosporins 13 at 25 °C. The nearly negligible contribution of each cephalosporin by itself was subtracted in each case. The ensuing conformational changes were then monitored for 96 h.
BacteriaThe strain panel was comprised of S. aureus COL, S. aureus COLVA, S. aureus VRS1 isolated in Michigan (16), and S. aureus VRS2 isolated in Pennsylvania (17). All four strains were obtained from the Network on Antimicrobial Resistance in Staphylococcus aureus. Also screened was a clinical isolate of vancomycin-resistant Enterococcus faecalis (E. faecalis 99) obtained from Wayne State University Medical Center.
Susceptibility TestingMICs were determined four times in each case by a broth microdilution method using cation-adjusted Mueller-Hinton II broth and procedures from the National Committee for Clinical Laboratory Standards (NCCLS) (18).
Solubility of the Acyl-enzyme Species of Cephalosporins 13 and PBP 2aA typical reaction mixture (100 µl) contained 9 µM PBP 2a and 20 µM cephalosporin 1, 2, or 3. The mixture was incubated at room temperature for 30 min in 25 mM Hepes, 1 M NaCl (pH 7.0) buffer. Aliquots (8 µl) of the mixture were diluted 3-fold with the same buffer and incubated for different time intervals (096 h). The samples were centrifuged (14,000 rpm) to pellet any protein that might have precipitated (none was visually detected). SDS sample buffer (15 µl) was added to the supernatant, and the mixture was boiled for 3 min and loaded onto a 10% SDS-polyacrylamide gel. PBP 2a (3 µM), in the absence of the cephalosporins, was run alongside as a control. The gel was treated with Coomassie Blue, destained, and scanned (Epson Perfection, 3200 Photo). The density of each PBP 2a band corresponding to a different time point was determined using ImageJ software. The results for each cephalosporin indicated that the acyl-enzyme complex remained fully soluble over the course of 4 days.
Nondenaturing PAGE of the Acyl-enzyme Species of Cephalosporins 13 and PBP 2aA typical reaction mixture (60 µl) contained 9 µM PBP 2a and 20 µM cephalosporins 13 in 25 mM Hepes, 1 M NaCl (pH 7.0) buffer. Aliquots (8 µl) of the mixture were diluted 3-fold with the same buffer and incubated for different time intervals (096 h). Nondenaturing sample buffer (15 µl) was added to aliquots of the sample, loaded onto a 5% nondenaturing polyacrylamide gel, and run alongside two controls, PBP 2a (3 µM) by itself and PBP 2a (3 µM) in the presence of ceftazidime (15 µM). Gels were run at 4 °C after 3, 24, and 96 h to monitor the occurrence of any oligomerization within the three samples. Each gel was treated with Coomassie Blue, destained, and scanned (Epson Perfection, 3200 Photo). The density of each PBP 2a band corresponding to the acyl-PBP 2a species (in the presence of cephalosporins 13) was compared with the two controls. There was no difference in the appearance of the bands for PBP 2a bound to antibiotics 13, for PBP 2a bound to ceftazidime, or for PBP 2a by itself. The results indicated that the acyl-enzyme species for each antibiotic or PBP 2a by itself did not form oligomers over the course of 4 days.
| RESULTS AND DISCUSSION |
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-lactams follows this kinetic scheme.
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-lactam-sensitive PBPs (1923). The deacylation rate constants (k3) are slower yet, with t
of 2677 h. Furthermore, the dissociation constants (Ks) for the preacylation complexes are elevated to values that preclude in vivo saturation of PBP 2a. Hence, these
-lactams (as with other examples) are ineffective PBP 2a inhibitors both by their modest dissociation constants and attenuated rate constants for acylation. A similar trend was noted for PBP 5 of Enterococcus faecium with some
-lactam antibiotics (24).
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Two additional data are noteworthy. First, the k2 values for these compounds are comparable with those for the other cephalosporins (Table 1). Hence, their improved biological activity does not result from enhanced acylation of PBP 2a for the antibiotic by itself. Second, in contrast to the three other cephalosporins, we could not detect any deacylation of the ABRI cephalosporin acyl-enzyme species over 3 days of monitoring. Therefore, these cephalosporins acylate PBP 2a and give a complex that does not allow for the recovery of PBP 2a activity. This lack of deacylation may also contribute to the effectiveness of these antibiotics.
Returning to the issue of the small values for k2/Ks, it is conceivable that the copy number of PBP 2a (
800 molecules/S. aureus bacterium (25)) is so low that even these rather modest k2/Ks parameters are sufficient for inhibition of PBP 2a (and anti-MRSA activity). However, our earlier findings with a bacterial cell wall mimetic indicated that the active site opening of PBP 2a, from a closed to an open conformation, was enabled by the binding of the cell wall mimetic to an allosteric site outside of the transpeptidase active site (10). This possibility was considered for the ABRI cephalosporins.
Compound 4 is an analogue of a fragment of the S. aureus cell wall, whose synthesis was reported recently by our laboratory (10). Compound 4 possesses the N-acetylglucosamine-N-acetylmuramic acid disaccharide backbone of the cell wall. To the N-acetylmuramic acid residue is appended the pentapeptide (N-acetylmuramic acid-L-Ala-
-D-Gln-L-Lys-D-Ala-D-Ala) linker unit that is typical of S. aureus. The peptidoglycan of S. aureus also has a pentaglycyl moiety appended to the L-Lys side chain that participates in the PBP 2a-catalyzed cross-linking reaction. The omission of the pentaglycyl segment from the structure of 4 was deliberate, since we did not want 4 to be turned over by PBP 2a.
The results for the ABRI cephalosporins with PBP 2a in the presence of compound 4 are intriguing (Table 1). As the concentration of the cell-wall analogue increased, the values for k2/Ks increased for each of antibiotics 13. The Kd (dissociation constant) for interaction of compound 4 outside the transpeptidase active site of PBP 2a is 1.2 ± 0.2 mM (10). We argued previously that the effective concentrations of the cell wall components that the PBPs experience on the bacterial cytoplasmic membrane are high (an entropic effect) (26). However, it is likely that the Kd value for a larger fragment of peptidoglycan than compound 4 (i.e. a true polymeric cell wall) or for an analogue with the pentaglycyl moiety appended to the L-Lys residue would be lower (more favorable) than those measured for compound 4. Nonetheless, compound 4 is a useful surrogate for the bacterial cell wall, since it does bind to the requisite allosteric binding site outside of the active site with demonstrated saturation (10). The results with compound 4 indicate that PBP 2a is predisposed to enhanced inhibition by the ABRI cephalosporins in the presence of the cell wall. It is important to note that these effects are consistent and set trends for all three cephalosporins (Table 1). As the concentration of the cell wall fragment increases, the values of k2 increase (enhance acylation), and those of Ks decrease (more favorable binding). The absolute magnitude of these parameters is not as important (since compound 4 is a mere cell wall surrogate) as the consistent trends that they establish. Despite the fact that compound 4 is not a perfect substitute for the cell wall of S. aureus, the values measured for k2/Ks at concentrations of 4 that approach saturation (>Kd) of PBP 2a are within the range that should be significant in vivo (i.e. 20004000 M1 s1) (27). We believe that these values should even be larger in the presence of a true polymeric cell wall, and they would contribute to the action of the novel cephalosporins in vivo.
Our previous study described time-dependent changes in the CD spectrum of PBP 2a in the presence of oxacillin and ceftazidime, during the first few t
values of the acylation process, documenting the existence of conformational changes during the acylation event. This was followed by the ultimate restoration to the CD spectrum of the mixture to that of the active enzyme, indicative of the completion of the deacylation event in due time (6). A similar set of changes in the CD spectrum is observed for PBP 2a in the presence of cephalosporins 13 (Fig. 2), except the process for the onset is more rapid, and there is no recovery to the native state. The issue of lack of recovery of activity (and of the coincident altered CD spectrum) is addressed below. However, we were able to fit the data from the early parts of the CD spectra to a first-order process for the conformational change. In essence, the formation of the noncovalent complex of the antibiotic and PBP 2a sets off the conformational change that leads to acylation of the active site. The limitation of this method is the fact that the CD spectral acquisition is a relatively slow process, and we had as few as four data points for the extraction of the rate constants. As such, these rate constants should be considered approximations. However, it is interesting that the observed first-order rate constants from the CD measurements (kobs of 0.004, 0.002, and 0.001 s1 for antibiotics 1, 2, and 3, respectively) are close to the corresponding values for the first-order acylation rate constants (k2, in the absence of compound 4; Table 1) of the respective antibiotics.
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Parenthetically we state here that since the change in the PBP 2a CD spectrum is also seen with penicillins (6), the effect that we observe with cephalosporins 13 cannot be attributed to the well characterized tautomerization of the dihydrothiazine double bond of the cephalosporins upon acylation of the enzyme (32, 33). Furthermore, the chromophore for the cephalosporins is weak, compared with the signals from the protein, such that it does not influence any of the events seen in Fig. 2 (see the flat lines at the top of A, C, and E).
One other conceivable explanation for the change in the CD of PBP 2a upon exposure to antibiotics 13 could be precipitation of the acylenzyme species from solution. The lack of observation of enhanced noise anticipated for protein precipitation in the course of the CD data acquisition argued against this possibility. Nonetheless, as described under "Experimental Procedures," we performed a specific experiment to show that concentration of the acylated PBP 2a in solution did not diminish over 4 days of incubation (data not shown). There was no evidence for any protein precipitation. Another conceivable contributor to the CD changes could be aggregation or oligomerization of the protein on acylation by the antibiotics over the course of the 4 days of monitoring. Admittedly, this was a remote possibility, but we investigated it by monitoring the acylated proteins over the duration of the 4 days by nondenaturing gels. PBP 2a is acylated by these antibiotics and remains stably in solution as a monomeric protein (data not shown). The essence of the observations of the data of Fig. 2 is the fact that these novel cephalosporins rapidly (in a matter of minutes, as opposed to over the course of hours seen with other cephalosporins) cause a conformational change necessary for access to the active site. This feature differentiates these antibacterials from other cephalosporins (including the clinically used cefepime and ceftazidime) studied by us previously (6). Furthermore, this process follows first-order kinetics, also inconsistent with the possibility of aggregation or oligomerization.
The minimum inhibitory concentrations (MICs) of antibiotics 13 were determined with four MRSA strains (Table 2). S. aureus COL (34) is a well characterized MRSA strain whose entire genome has been sequenced (35). S. aureus COLVA was engineered by transferring the vancomycin resistance determinants from the clinical staphylococcal isolate VRS1 into S. aureus COL (36), and it is resistant to both oxacillin (methicillin) and vancomycin, as reflected by the MIC data. The strains S. aureus VRS1 and S. aureus VRS2 are vancomycin-resistant MRSA strains isolated in Michigan (16) and in Pennsylvania (17). These are the first ever vancomycin-resistant MRSA strains isolated from the clinic. An MIC value of 4 µg/ml or less for vancomycin is highly desirable. We also include in this table the MIC for a vancomycin-resistant clinical isolate of E. faecalis (E. faecalis 99). Oxacillin, not methicillin, is used for treatment of infections from methicillin/oxacillin-susceptible staphylococci (oxacillin MICs
2 µg/ml for S. aureus and
0.25 µg/ml for coagulase-negative staphylococci) infections in the United States. Both antibiotics produce similar MICs against various staphylococcal strains. As expected, the MRSA strains are oxacillin-resistant (MIC > 128 µg/ml).
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-lactam antibiotics against oxacillin-susceptible strains of S. aureus (
8 µg/ml for cefepime, ceftazidime, and aztreonam and
4 µg/ml for imipenem) (37). The data presented here indicate that cephalosporins 13 have good activities against strains of MRSA (and one strain of vancomycin-resistant E. faecalis that was also examined). We present evidence that the dissociation constants for the preacylation complexes of PBP 2a with these antibiotics are lower, but the first-order acylation rate constants for PBP 2a by these antibiotics are not any different than those of other cephalosporins (Table 1). Whereas the pseudo-second-order rate constants (k2/Ks) for the encounter of these cephalosporins and PBP 2a are small, we have documented that in the presence of an analogue of the cell wall (compound 4), this meaningful parameter, a quantitative measure of enzyme inactivation efficacy, is enhanced significantly. This enhancement takes the k2/Ks values for cephalosporins 13 to a level that is clinically relevant (27). In addition, it is likely that the effect of the actual S. aureus polymeric cell wall on enhancement of the k2/Ks values would be even more than what we have measured with the surrogate compound 4.
Furthermore, we have documented that interactions of cephalosporins 13 with PBP 2a cause a rapid adjustment in the conformation of the protein, such that access to the active site of the enzyme becomes more facile. We surmise that, based on the measured k2/Ks values, the interactions of the cell wall with PBP 2a might be required for the antibiotics to be effective. Hence, cephalosporins 1-3 might conceivably co-opt the existing interactions of the cell wall with PBP 2a to achieve access to the active site. We add that Lu et al. (9) analyzed one
-lactam antibiotic that has shown activity against MRSA previously. The kinetics of interactions of this antibiotic with PBP 2a were evaluated by mass spectrometry (k2 = 0.39 s1, Ks = 0.22 mM, k2/Ks = 1750 M1 s1) (9). Whereas the Ks value for this antibiotic was 1 order of magnitude higher than those studied by us in this respect, the measured k2 was more rapid in that case. The effect of the cell wall fragments on PBP 2a was not evaluated in that study; nor were the issues of conformational changes investigated.
The interaction between the cell wall and PBP 2a is believed to facilitate the requisite conformational change. We have documented such an allosteric binding site for the cell wall, which binds to PBP 2a in a saturable manner. Although the knowledge of the location of this binding site and how this binding event alters the protein structure is presently lacking, we speculate that the collective three-dimensional structures of cephalosporins 13, especially the abundance of heteroatoms at the C3 and C7 functionalities, are the contributing factors.
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1 To whom correspondence should be addressed: Dept. of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556. Tel.: 574-631-6652; Fax: 574-631-2933; E-mail: mobashery{at}nd.edu.
2 The abbreviations used are: MRSA, methicillin-resistant S. aureus; PBP, penicillin-binding protein; FL, fluorescent; MIC, minimum inhibitory concentration. ![]()
| ACKNOWLEDGMENTS |
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| REFERENCES |
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