Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.C600038200 on April 10, 2006

J. Biol. Chem., Vol. 281, Issue 22, 15033-15036, June 2, 2006
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/22/15033    most recent
C600038200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freibaum, B. D.
Right arrow Articles by Counter, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freibaum, B. D.
Right arrow Articles by Counter, C. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

hSnm1B Is a Novel Telomere-associated Protein*

Brian D. Freibaum and Christopher M. Counter1

From the Department of Pharmacology and Cancer Biology, Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina 27710

Received for publication, February 21, 2006


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Artemis, a member of the beta-CASP family, has been implicated in the regulation of both telomere stability and length. Prompted by this, we examined whether the other two putative DNA-binding members of this family, hSnm1A and hSnm1B, may associate with telomeres. hSnm1A was found to not interact with the telomere. Conversely, hSnm1B was found to associate with telomeres in vivo by both immunofluorescence and chromatin immunoprecipitation. Furthermore, the C terminus of hSnm1B was shown to interact with the TRF homology domain of TRF2 indicating that hSnm1B is likely recruited to the telomere via interaction with the double-stranded telomere-binding protein TRF2.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
The ends of human chromosomes are capped and protected by a DNA-protein structure termed the telomere. The DNA portion of human telomeres is composed of a G-rich repeat (TTAGGG) that extends past the complementary C-rich strand, forming a 3' extension. This extension has been found by electron microscopy to loop back and invade the double-stranded region, forming a large loop structure termed the t-loop (1). The protein portion of telomeres is composed of a core of six proteins termed the telosome or shelterin (2–4), three of which directly bind telomeric DNA, TRF1 (5), TRF2 (6, 7), and hPot1 (8), and three that associate with the DNA-binding proteins, Tin2 (9), Tpp1 (10–12), and Rap1 (13). TRF1 and TRF2 bind double-stranded telomeric DNA (7), whereas hPot1 binds the single-stranded region of the telomere (8). In addition to this core complex, many other proteins are known to associate and function at the telomere, albeit at a smaller concentration and often indirectly through binding to TRF1 or TRF2 (4).

One protein that influences the stability of the telomere but is not a member of the described core telomeric complex is Artemis. This protein is a member of the beta-CASP family of proteins that contain a unique metallo-beta-lactamase domain that hydrolyzes nucleic acids (14). Artemis is well established to play a role in non-homologous recombination where it forms a complex with DNA-PKCS, which cleaves the intermediate hairpin loop structure formed during V(D)J recombination and non-homologous end joining (15). However, Artemis-deficient mice have increased telomeric fusions, suggesting that this family of proteins plays a role in telomere structure or function (16). Indeed, Artemis deficient cell lines have increased rates of telomeric shortening as well as rapid accumulation of anaphase bridges (17).

Five mammalian proteins belonging to the beta-CASP family have been identified, two of which hydrolyze RNA (18–21), whereas the remaining three, Artemis, Snm1A, and Snm1B, are believed to function solely on DNA substrates (22). Snm1A localizes to DNA double-strand breaks after ionizing radiation; although cells lacking Snm1A are sensitive only to mitomycin C and not ionizing radiation (23, 24). Snm1A co-localizes with the DNA damage response protein 53BP before and after exposure to ionizing radiation (23). Snm1A is also highly up-regulated during mitosis and is believed to function as a checkpoint protein during early mitosis (25, 26). Snm1A knock-out mice have increased susceptibility to infection and tumorigenesis (27), further suggesting a role in DNA repair and proper immune function similar to Artemis.

Snm1B is the least well understood of the DNA-binding beta-CASP family members. Knock-out of Snm1B in chicken cells and small interfering RNA against Snm1B in human cells both result in mild sensitivity to interstrand cross-linking agents (28, 29). Knockdown of Snm1B by small interfering RNA induces an increase in aberrant metaphase morphology in human cells (29).

The importance of Artemis on telomere stability and telomere length regulation led us to examine whether the other two DNA-binding members of the beta-CASP family, human (h)2 Snm1A and hSnm1B associate with telomeres.


   EXPERIMENTAL PROCEDURES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
RESULTS
 DISCUSSION
 REFERENCES
 
Cell Culture—The transformed human embryonic kidney cell line, 293T, was cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum.

Plasmids—hSnm1A was PCR-amplified from plasmid clone (ATCC clone 7286032) to include a 5'-FLAG epitope sequence and subcloned into pBabePuro. YFP was PCR amplified from pEYFP-C3 (Clontech) and subcloned in frame at the N terminus of FLAG-hSnm1A to generate YFP-FLAG-hSnm1A. The sequence of hSnm1A was verified by DNA sequencing.

hSnm1B was obtained by reverse transcriptase PCR amplification of dT-primed 293T RNA and subcloned in frame into pEGFP-C1 (Clontech). The sequence of hSnm1B was verified by DNA sequencing. hSnm1B or truncation mutants that result in fragments from amino acids 413–532, 463–532, 496–532, and 363–495 were generated by PCR and subcloned in frame with GFP lacking a stop codon, generating pBabePuro-GFP-hSnm1B or mutants thereof.

pcDNA3-myc-TRF2 was a kind gift from Dominique Broccoli. The following truncation mutants of TRF2 were generated by PCR (numbers refer to the corresponding amino acid region amplified), N-terminally tagged with myc by cloning into pCMV-myc (Clontech): TRF2-(2–100), TRF2-(101–200), TRF2-(301–400), TRF2-(401–500), TRF2-(2–300), and TRF2-(269–445).

Visualization of YFP-tagged hSnm1A, GFP-tagged hSnm1B, and TRF2—293T cells grown on coverslips coated with 100 mg/ml poly-D-lysine, Mr > 300,000 (Sigma), were transiently transfected with either pBabe-YFP-FLAG-hSnm1A or pBabe-GFP-hSnm1B using FuGENE 6 (Roche Diagnostics) according to the manufacturer's protocols. After 48 h, 293T cells were fixed in 3.7% formaldehyde in PBS for 10 min at room temperature. To visualize TRF2, 293T cells were then permeabilized with 0.5% Nonidet P-40 in 1x PBS and incubated with the anti-human TRF2 monoclonal antibody (Imgenex IMG-124A) at 1:5000 dilution. The primary antibody was detected with the rhodamine (TRITC)-conjugated donkey anti-mouse IgG antibody (Jackson ImmunoResearch Laboratories). The cells were then washed twice with PBS, mounted, and observed using the 100x objective lens on an Olympus IX70 confocal microscope.

Chromatin Immunoprecipitation Assay—Chromatin immunoprecipitations were performed as described previously (30) with the following modifications: a Branson sonifier microtip (Branson Ultrasonics) was used for sonication (output 3; duty cycle, 30% for five 10 s bursts), after which insoluble material was pelleted by microcentrifugation (13,000 x g for 5 min at 4 °C), and the remaining lysate was diluted in lysis buffer (1:2). 30 µl of 50% slurry of GammaBind G-Sepharose (Amersham Biosciences) was added to the lysate and incubated at 4 °C for 1 h to preclear the lysate. The lysate was then transferred to new tubes and immunoprecipitated overnight with 4 µg of anti-GFP monoclonal antibody (Roche Diagnostics). Finally, dot blots were hybridized with a 32P-labeled oligonucleotide telomeric probe (T2AG3)4 in Church's buffer overnight at 50 °C followed by two washes with 4x SSC containing 0.1% SDS. After 5 days of phosphoimaging the blots were then stripped and probed with an {alpha} satellite probe (derived from plasmid p82H) (31) in Church's buffer overnight at 60 °C followed by two washes with 0.1x SSC containing 0.1% SDS. Hybridization of the probes was confirmed with 10 µg of total genomic DNA blotted on each membrane.

Immuoprecipitations—293T cells were seeded in 6-cm tissue culture dishes and transfected with 3 µg of plasmid. Nuclear lysates were collected 48 h later and immunoprecipitated overnight with 4 µg of anti-GFP monoclonal antibody (Roche Diagnostics). Bound proteins were then collected on GammaBind G-Sepharose, washed in radioimmune precipitation assay buffer, and denatured by boiling in SDS sample buffer. The proteins were then separated by polyacrylamide gel electrophoresis and immunoblotted with the appropriate antibody. Anti-myc antibody (Invitrogen) was used to detect myc-TRF2 and myc-TRF2 truncation mutants. Anti-GFP monoclonal antibody (Roche Diagnostics) and rabbit anti-GFP polyclonal antibody (Santa Cruz Biotechnology) were used to detect YFP-FLAG-hSnm1A, GFP-hSnm1B, and GFP-hSnm1B truncation mutants.


Figure 1
View larger version (108K):
[in this window]
[in a new window]
  		FIGURE 1.
hSnm1B, but not hSnm1A, localizes to the telomere. 293T cells were plated on coverslips and transiently transfected with either YFP-FLAG-hSnm1A (left) or GFP-hSnm1B (right). The cells were then fixed, permeabilized, and incubated with TRF2 monoclonal antibody. The TRF2 antibody was visualized with the rhodamine (TRITC)-conjugated donkey anti-mouse IgG antibody (top). YFP-FLAG-hSnm1A was found to localize to punctate nuclear bodies (center left) but did not co-localize with TRF2 (bottom left). GFP-hSnm1B was found to localize to punctate nuclear bodies (center right) that co-localize with TRF2 (bottom right).

 

   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
DISCUSSION
 REFERENCES
 
hSnm1B, but Not hSnm1A, Localizes to the Telomere—Perturbing Artemis function in mice leads to telomere defects (16, 17). However, Artemis is only one of three putative DNA-binding proteins containing beta-lactamase domains, the remaining two being hSnm1A and hSnm1B (22). hSnm1A and hSnm1B have also been found to localize to discrete punctate nuclear bodies (28), reminiscent of punctate nuclear localization seen with telomeric proteins (7). We therefore tested whether hSnm1A or hSnm1B localized to the telomere. To examine whether these proteins associate with telomeres in human cells, we assayed whether ectopic hSnm1A or hSnm1B co-localize with the known double-stranded telomeric-binding protein TRF2. Specifically, human 293T cells were transfected with either FLAG-hSnm1A or hSnm1B N-terminally tagged with YFP or GFP, respectively, for visualization purposes. The localization of these fluorescent proteins was then compared with that of endogenous TRF2, as measured by immunofluorescence. As reported previously, both YFP-FLAG-hSnm1A and GFP-hSnm1B localized to discrete punctate nuclear bodies (28). However, only GFP-hSnm1B co-localized with endogenous TRF2 at the telomere (Fig. 1). These data support the notion that hSnm1B associates with telomeres.

To independently confirm the localization of hSnm1B to telomeres, we tested whether hSnm1B associates with telomeric chromatin in vivo by chromatin immunoprecipitation. GFP-hSnm1B was transiently expressed in 293T cells for 48 h, and cells were cross-linked, lysed, the chromatin sheared, and the tagged protein immunoprecipitated with GFP antibody. To detect associated telomeric DNA, DNA was purified from the immunoprecipitate, blotted on a membrane, and hybridized with a telomeric probe to determine specific protein-telomere interactions. As a control for nonspecific association with telomeric DNA, GFP and YFP-FLAG-hSnm1A, both of which are not found to be associated with telomeric DNA by immunofluorescence (Fig. 1 and data not shown) were similarly expressed in 293T cells and analyzed for association with telomeric DNA in parallel with GFP-hSnm1B. We also stripped and rehybridized the membranes with a centromeric probe to control for nonspecific protein-DNA interactions. Finally, to ensure that interactions with telomeric DNA were specific, non-cross-linked controls were also subject to chromatin immunoprecipitation (Fig. 2A). We found that hSnm1B co-immunoprecipitated specifically with telomeric DNA (Fig. 2, A and B), as a strong signal was detected when hSnm1B immunoprecipitates were hybridized with a telomeric probe but not the centromeric probe (Fig. 2, A and C), and only background signal was detected with the telomeric probe in the absence of crosslinking. Similarly, immunoprecipitates from control GFP and YFP-FLAG-hSnm1A gave background signals with both the telomeric and centromeric probes (Fig. 2, A–C). We thus conclude that hSnm1B associates with telomeres in vivo.

The C Terminus of hSnm1B Binds TRF2—Given that the immunofluorescence and chromatin immunoprecipitation experiments demonstrate that hSnm1B associates with telomeres in vivo, we sought to determine how hSnm1B is tethered to telomeres. We explored the possibility that hSnm1B localizes to the telomere via protein-protein interaction. Since TRF1, TRF2, and hPot1 are the core proteins directly associating with telomeric DNA, and these proteins are known to serve as a scaffold for proteins to telomeres (4), we first tested whether they associate with hSnm1B. 293T cells were transiently transfected with GFP-hSnm1B, immunoprecipitated with an anti-GFP monoclonal antibody, and blotted with an anti-TRF1, TRF2, or hPot1 antibody to detect for an association with endogenous TRF1, TRF2, or hPot1, respectively. Of these, only TRF2 appeared to co-immunoprecipitate with hSnm1B (Fig. 3A and data not shown). This interaction was specific to hSnm1B, as transiently expressed YFP-FLAG-hSnm1A did not co-immunoprecipitate with TRF2 (Fig. 3C).


Figure 2
View larger version (26K):
[in this window]
[in a new window]
  		FIGURE 2.
hSnm1B binds telomeric DNA in vivo. A, 293T cells were transiently transfected with GFP alone, YFP-FLAG-hSnm1A, GFP-hSnm1B, or GFP-hSnm1B-(363–495) and either treated with formaldehyde to crosslink proteins and DNA or left untreated as a control. The cells were then subjected to chromatin immunoprecipitation with an anti-GFP monoclonal antibody followed by southern blotting with a telomeric probe. As a control, membranes were also stripped and re-hybridized with a centromeric probe to determine nonspecific DNA interactions. B and C, graphical quantification of telomeric (B) or nonspecific centromeric hybridization (C) signals of the indicated chromatin-immunoprecipitated proteins. Only hSnm1B specifically immunoprecipitated telomeric DNA.

 
To further explore the association of TRF2 with hSnm1B, we mapped the TRF2-binding domain on hSnm1B. We focused on the C terminus of hSnm1B, as this region is not conserved with hSnm1A, which does not associate with telomeres. Three progressively larger N-terminal truncation mutants of hSnm1B (413–532, 463–532, 496–532) were generated and N-terminally tagged with GFP (Fig. 3B), transiently expressed in 293T cells, after which the mutant proteins were immunoprecipitated with GFP monoclonal antibody and immunoblotted with an anti-TRF2 antibody to detect endogenous TRF2 (Fig. 3C). All three progressively shorter C-terminal fragments co-immunoprecipitated with TRF2, including GFP-hSnm1B-(496–532), the polypeptide encompassing the last 37 amino acids of the protein. This suggests that the most terminal amino acids are responsible for the TRF2 interaction. In agreement, a mutant lacking these C-terminal 37 amino acids, GFP-hSnm1B-(363–495), was not capable of binding TRF2.


Figure 3
View larger version (48K):
[in this window]
[in a new window]
  		FIGURE 3.
TRF2 interacts with the C terminus of hSnm1B. A, 293T cells were transiently transfected with GFP-hSnm1B and immunoprecipitated with GFP monoclonal antibody. The resultant immunoprecipitates were separated on a polyacrylamide gel and blotted with either GFP monoclonal antibody (top) or TRF2 monoclonal antibody (bottom). GFP-hSnm1B was found to co-immunoprecipitate with endogenous TRF2. Control mock-transfected cells were unable to co-immunoprecipitate TRF2. B, to elucidate the TRF2-binding domain, the indicated GFP-tagged hSnm1B truncation mutants were generated. C, 293T cells were transiently transfected with YFP-FLAG-hSnm1A, GFP-hSnm1B, or a GFP-tagged truncation mutant of hSnm1B, followed by immunoprecipitation with GFP monoclonal antibody, separation on a polyacrylamide gel, and blotting with either GFP polyclonal antibody (top) or TRF2 monoclonal antibody (bottom). All of these mutants were able to co-immunoprecipitate endogenous TRF2 except mutant GFP-hSnm1B (363–495). YFP-FLAG-hSnm1A was unable to co-immunoprecipitate TRF2.

 
We next tested whether the TRF2-binding region of hSnm1B was required for telomere association in vivo by chromatin immunoprecipitation. Specifically, the GFP-hSnm1B-(363–495) was transiently expressed in 293T cells, immunoprecipitated with an anti-GFP monoclonal antibody, and associated telomeric DNA detected by hybridization with a telomeric probe. Unlike full-length hSnm1B, the TRF2-binding mutant did not co-immunoprecipitate with telomeric DNA (Fig. 2). Based on these in vitro and in vivo experiments, we conclude that hSnm1B is localized to telomeres via an interaction with TRF2.

hSnm1B Interacts with the TRFH Domain of TRF2—Because TRF2 is known to interact with a number of telomere-binding proteins, we were interested in determining what domain of TRF2 was required for the interaction with hSnm1B. A panel of N-terminal myc-tagged TRF2 truncation mutants were generated (Fig. 4A) and assayed for association with hSnm1B. Specifically, 293T cells were transiently transfected with wild-type or mutant myc-TRF2 and GFP-hSnm1B, after which hSnm1B was immunoprecipitated with an anti-GFP monoclonal antibody followed by immunoblotting with an anti-myc antibody to detect associated ectopic TRF2. Only full-length TRF2 and a truncation mutant corresponding to the first 300 amino acids of TRF2, myc-TRF2-(2–300), were able to interact with GFP-hSnm1B (Fig. 4B). This mutant encompasses both the N-terminal basic domain of TRF2 as well as the TRF2 homology domain, which is essential for dimerization and for known protein-protein interactions (32). The basic domain is not sufficient for interaction with GFP-hSnm1B, as a mutant containing the entire domain, myc-TRF2-(2–100), is unable to be immunoprecipitated with GFP-hSnm1B (Fig. 4B). Thus, the TRF homology domain of TRF2 is most important for interacting with hSnm1B. Interestingly, far more hSnm1B was immunoprecipitated when TRF2 or the hSnm1B-binding domain of TRF2 was overexpressed (Fig. 4B), perhaps suggesting that binding of TRF2 either increases the stability of hSnm1B or produces a conformational change within hSnm1B that fosters immunoprecipitation.


Figure 4
View larger version (40K):
[in this window]
[in a new window]
  		FIGURE 4.
The TRF2 homology domain is required for interaction with hSnm1B. A, to elucidate the hSnm1B interaction domain on TRF2, the indicated N-terminal myc epitope-tagged TRF2 truncation mutants were generated. B, 293T cells were transiently transfected with GFP-hSnm1B and either myc-TRF2 or a myc-tagged TRF2 truncation mutant, followed by immunoprecipitation with GFP monoclonal antibody. The immunoprecipitates were then separated on a polyacrylamide gel and blotted with either GFP monoclonal antibody (top) or myc antibody (middle). 5% of the nuclear extracts were used for inputs to verify expression of the TRF2 truncation mutants (bottom). hSnm1B was found to strongly interact only with wild-type TRF2 and the region of TRF2 containing the complete TRF2 homology domain.

 

   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
REFERENCES
 
We have shown that hSnm1B is a novel telomere-associated protein. In addition, we have shown that hSnm1B co-immunoprecipitates with the double-stranded telomere-binding protein TRF2. This interaction occurs via a TRF2 interaction domain on the C terminus of hSnm1B. We speculate that hSnm1B is recruited to the telomere via the interaction with TRF2, although we have not yet determined whether this interaction is direct or indirect. What hSnm1B does at the telomere can only be speculated at this point. On one hand, since Artemis can cleave single-stranded DNA loop structures (15), it is possible that hSnm1B may play a role in processing the telomeric t-loop structure. Alternatively, as the budding yeast SNM1 possesses 5'-exonuclease activity in vitro (33, 34), perhaps hSnm1B generates the single-stranded 3' overhang at the end of the telomere. Nevertheless, the association of hSnm1B with telomeres argues that this protein is likely performing some function critical to telomere structure or function.


   FOOTNOTES
 
* This work was supported by National Institutes of Health Grant CA82481. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

1 A Leukemia and Lymphoma Society Scholar. To whom correspondence should be addressed. Tel.: 919-684-9890; Fax: 919-684-8958; E-mail: count004{at}mc.duke.edu.

2 The abbreviations used are: h, human; YFP, yellow fluorescent protein; GFP, green fluorescent protein; PBS, phosphate-buffered saline; TRITC, tetramethylrhodamine isothiocyanate. Back


   ACKNOWLEDGMENTS
 
We thank Brooke Ancrile for technical assistance; members of the Counter laboratory, Shawn Ahmed, and Bettina Meier for helpful discussions; and last, Dominique Broccoli for generously providing pcDNA3-myc-TRF2.



   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 

  1. Griffith, J. D., Comeau, L., Rosenfield, S., Stansel, R. M., Bianchi, A., Moss, H., and de Lange, T. (1999) Cell 97, 503–514[CrossRef][Medline] [Order article via Infotrieve]
  2. Wright, J. H., Gottschling, D. E., and Zakian, V. A. (1992) Genes. Dev. 6, 197–210[Abstract/Free Full Text]
  3. Liu, D., O'Connor, M. S., Qin, J., and Songyang, Z. (2004) J. Biol. Chem. 279, 51338–51342[Abstract/Free Full Text]
  4. de Lange, T. (2005) Genes Dev. 19, 2100–2110[Abstract/Free Full Text]
  5. Smith, S., and de Lange, T. (1997) Trends Genet. 13, 21–26[CrossRef][Medline] [Order article via Infotrieve]
  6. Bilaud, T., Brun, C., Ancelin, K., Koering, C. E., Laroche, T., and Gilson, E. (1997) Nat. Genet. 17, 236–239[CrossRef][Medline] [Order article via Infotrieve]
  7. Broccoli, D., Smogorzewska, A., Chong, L., and de Lange, T. (1997) Nat. Genet. 17, 231–235[CrossRef][Medline] [Order article via Infotrieve]
  8. Baumann, P., and Cech, T. R. (2001) Science 292, 1171–1175[Abstract/Free Full Text]
  9. Kim, S. H., Kaminker, P., and Campisi, J. (1999) Nat. Genet. 23, 405–412[CrossRef][Medline] [Order article via Infotrieve]
  10. Liu, D., Safari, A., O'Connor, M. S., Chan, D. W., Laegeler, A., Qin, J., and Songyang, Z. (2004) Nat. Cell Biol. 6, 673–680[CrossRef][Medline] [Order article via Infotrieve]
  11. Ye, J. Z., Hockemeyer, D., Krutchinsky, A. N., Loayza, D., Hooper, S. M., Chait, B. T., and de Lange, T. (2004) Genes Dev. 18, 1649–1654[Abstract/Free Full Text]
  12. Houghtaling, B. R., Cuttonaro, L., Chang, W., and Smith, S. (2004) Curr. Biol. 14, 1621–1631[CrossRef][Medline] [Order article via Infotrieve]
  13. Li, B., Oestreich, S., and de Lange, T. (2000) Cell 101, 471–483[CrossRef][Medline] [Order article via Infotrieve]
  14. Aravind, L. (1999) In Silico Biol. 1, 69–91[Medline] [Order article via Infotrieve]
  15. Ma, Y., Pannicke, U., Schwarz, K., and Lieber, M. R. (2002) Cell 108, 781–794[CrossRef][Medline] [Order article via Infotrieve]
  16. Rooney, S., Alt, F. W., Lombard, D., Whitlow, S., Eckersdorff, M., Fleming, J., Fugmann, S., Ferguson, D. O., Schatz, D. G., and Sekiguchi, J. (2003) J. Exp. Med. 197, 553–565[Abstract/Free Full Text]
  17. Cabuy, E., Newton, C., Joksic, G., Woodbine, L., Koller, B., Jeggo, P. A., and Slijepcevic, P. (2005) Radiat. Res. 164, 53–62[CrossRef][Medline] [Order article via Infotrieve]
  18. Christofori, G., and Keller, W. (1988) Cell 54, 875–889[CrossRef][Medline] [Order article via Infotrieve]
  19. Takagaki, Y., Ryner, L. C., and Manley, J. L. (1989) Genes Dev. 3, 1711–1724[Abstract/Free Full Text]
  20. Gilmartin, G. M., and Nevins, J. R. (1989) Genes Dev. 3, 2180–2190[Abstract/Free Full Text]
  21. Takaku, H., Minagawa, A., Takagi, M., and Nashimoto, M. (2003) Nucleic Acids Res. 31, 2272–2278[Abstract/Free Full Text]
  22. Callebaut, I., Moshous, D., Mornon, J. P., and de Villartay, J. P. (2002) Nucleic Acids Res. 30, 3592–3601[Abstract/Free Full Text]
  23. Richie, C. T., Peterson, C., Lu, T., Hittelman, W. N., Carpenter, P. B., and Legerski, R. J. (2002) Mol. Cell. Biol. 22, 8635–8647[Abstract/Free Full Text]
  24. Dronkert, M. L., de Wit, J., Boeve, M., Vasconcelos, M. L., van Steeg, H., Tan, T. L., Hoeijmakers, J. H., and Kanaar, R. (2000) Mol. Cell. Biol. 20, 4553–4561[Abstract/Free Full Text]
  25. Zhang, X., Richie, C., and Legerski, R. J. (2002) DNA Repair (Amst.) 1, 379–390
  26. Akhter, S., Richie, C. T., Deng, J. M., Brey, E., Zhang, X., Patrick, C., Jr., Behringer, R. R., and Legerski, R. J. (2004) Mol. Cell. Biol. 24, 10448–10455[Abstract/Free Full Text]
  27. Ahkter, S., Richie, C. T., Zhang, N., Behringer, R. R., Zhu, C., and Legerski, R. J. (2005) Mol. Cell. Biol. 25, 10071–10078[Abstract/Free Full Text]
  28. Ishiai, M., Kimura, M., Namikoshi, K., Yamazoe, M., Yamamoto, K., Arakawa, H., Agematsu, K., Matsushita, N., Takeda, S., Buerstedde, J. M., and Takata, M. (2004) Mol. Cell. Biol. 24, 10733–10741[Abstract/Free Full Text]
  29. Demuth, I., Digweed, M., and Concannon, P. (2004) Oncogene. 23, 8611–8618[CrossRef][Medline] [Order article via Infotrieve]
  30. Cheung, P., Tanner, K. G., Cheung, W. L., Sassone-Corsi, P., Denu, J. M., and Allis, C. D. (2000) Mol. Cell 5, 905–915[CrossRef][Medline] [Order article via Infotrieve]
  31. Mitchell, A. R., Gosden, J. R., and Miller, D. A. (1985) Chromosoma 92, 369–377[CrossRef][Medline] [Order article via Infotrieve]
  32. Fairall, L., Chapman, L., Moss, H., de Lange, T., and Rhodes, D. (2001) Mol. Cell 8, 351–361[CrossRef][Medline] [Order article via Infotrieve]
  33. Li, X., and Moses, R. E. (2003) DNA Repair (Amst.) 2, 121–129
  34. Li, X., Hejna, J., and Moses, R. E. (2005) DNA Repair (Amst.) 4, 163–170

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
Y. Tatsumi, K. Ezura, K. Yoshida, T. Yugawa, M. Narisawa-Saito, T. Kiyono, S. Ohta, C. Obuse, and M. Fujita
Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres
Genes Cells, October 1, 2008; 13(10): 1045 - 1059.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. D. Freibaum and C. M. Counter
The Protein hSnm1B Is Stabilized When Bound to the Telomere-binding Protein TRF2
J. Biol. Chem., August 29, 2008; 283(35): 23671 - 23676.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Grillari, H. Katinger, and R. Voglauer
Contributions of DNA interstrand cross-links to aging of cells and organisms
Nucleic Acids Res., December 14, 2007; (2007) gkm1065v1.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/22/15033    most recent
C600038200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freibaum, B. D.
Right arrow Articles by Counter, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freibaum, B. D.
Right arrow Articles by Counter, C. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement