JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M601173200 on June 28, 2006

J. Biol. Chem., Vol. 281, Issue 35, 25502-25508, September 1, 2006
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/35/25502    most recent
M601173200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, M. D.
Right arrow Articles by Bycroft, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, M. D.
Right arrow Articles by Bycroft, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The PUB Domain Functions as a p97 Binding Module in Human Peptide N-Glycanase*

Mark D. Allen{ddagger}, Alexander Buchberger§1, and Mark Bycroft{ddagger}2

From the {ddagger}Centre for Protein Engineering, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom and the §Max Planck Institute of Biochemistry, Department of Molecular Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany

Received for publication, February 7, 2006 , and in revised form, June 27, 2006.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
The AAA ATPase p97 is a ubiquitin-selective molecular machine involved in multiple cellular processes, including protein degradation through the ubiquitin-proteasome system and homotypic membrane fusion. Specific p97 functions are mediated by a variety of cofactors, among them peptide N-glycanase, an enzyme that removes glycans from misfolded glycoproteins. Here we report the three-dimensional structure of the aminoterminal PUB domain of human peptide N-glycanase. We demonstrate that the PUB domain is a novel p97 binding module interacting with the D1 and/or D2 ATPase domains of p97 and identify an evolutionary conserved surface patch required for p97 binding. Furthermore, we show that the PUB and UBX domains do not bind to p97 in a mutually exclusive manner. Our results suggest that PUB domain-containing proteins constitute a widespread family of diverse p97 cofactors.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Glycoproteins of the secretory pathway that fail to fold correctly in the endoplasmic reticulum are retro-translocated to the cytosol for degradation by the ubiquitin-proteasome system in a process known as endoplasmic reticulum-associated protein degradation (1, 2). This pathway requires dedicated ubiquitin ligases and the AAA ATPase p97 (also called VCP, Cdc48) (1-3). p97 consists of an amino-terminal N domain and two AAA domains, D1 and D2. It forms a homohexameric, barrel-like structure consisting of two ring-shaped layers made of the D1 and D2 domains (4). The protein-extracting activity of p97 is believed to be the result of conformational changes that accompany nucleotide binding and hydrolysis (5, 6). The importance of functional p97 for endoplasmic reticulum-associated protein degradation is illustrated by the fact that mutations in p97 that are associated with the disorder inclusion body myopathy with Paget disease of the bone and fronto-temporal dementia cause endoplasmic reticulum-associated protein degradation defects (7, 8).

Prior to proteasomal degradation of retro-translocated, glycosylated proteins, N-linked oligosaccharide chains are removed by the enzyme peptide N-glycanase (PNGase)3 (9-11). The Saccharomyces cerevisiae PNGase homologue Png1 is 363 amino acids in length and contains a catalytic triad of cysteine, histidine, and aspartic acid residues typical of the transglutaminase-like superfamily of enzymes (12). It binds to the proteasomal targeting factor Rad23, thereby possibly linking glycan removal to proteasomal degradation (9).

Although the interaction between PNGase and Rad23 is evolutionary conserved in higher eukaryotes (13), differences appear to exist with respect to details of the interaction (14). Animal PNGases possess an additional amino-terminal extension that contains a PUB (also called PUG) domain, a protein module of unknown function found in many proteins linked to the ubiquitin-proteasome system on the basis of their domain architecture (15, 16) (Fig. 1). The presence of the PUB domain suggests that animal PNGases are subject to more complex regulation. Consistent with this notion, p97 and the putative retrotranslocation pore component Derlin-1 have recently been shown to interact with PNGase in mammalian cells, raising the intriguing possibility that endoplasmic reticulum-associated protein degradation substrates are deglycosylated by PNGase during their p97-mediated retro-translocation and subsequently targeted to the 26 S proteasome via the Rad23 homologue, HR23B (17-19). To gain insight into the role of the PUB domain, we solved the three-dimensional structure of the PUB domain of human PNGase and found that it is a novel p97 binding module containing a conserved p97 binding site.


   EXPERIMENTAL PROCEDURES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
RESULTS
 DISCUSSION
 REFERENCES
 
Structure Determination—DNA encoding the PUB domain of human PNGase (residues 11-109) was PCR amplified from a human cDNA library (Clontech) and cloned into a pRSETA (Invitrogen) derivative that expresses proteins fused to the lipoyl domain of Bacillus stearothermophilus dihydrolipoamide acetyltransferase. The resulting plasmid was transformed into Escherichia coli C41(DE3) cells. Cells were grown at 37 °C in Luria Bertani broth to mid log phase and induced with 1 mM isopropyl-1-thio-beta-D-galactopyranoside. The temperature was then reduced to 25 °C, and the cells were grown for a further 16 h. Cells were lysed by sonication, and the fusion protein was purified using a nickel-nitrilotriacetic acid Superflow affinity column (Amersham Biosciences). Following cleavage with thrombin (4 h at 30 °C), the PUB domain was further purified by ion exchange chromatography using a Source Q column (Amersham Biosciences) and subsequent gel filtration using a Superdex 75 HR column (Amersham Biosciences). A PUB domain containing L66M, L75M, and L87M mutations was created using the QuikChange II XL kit (Stratagene) to facilitate selenium incorporation. Selenomethionine-substituted mutant protein was prepared exactly as above except that cells were grown in M9 minimal medium supplemented with seleno-methionine. Crystals were grown using the sitting drop vapor diffusion method at 290 K with a reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris, pH 8.5, 30% polyethylene glycol 4000 using 20% glycerol as a cryoprotectant. Native and MAD data sets were collected at ID14-4, European Synchrotron Radiation Facility, Grenoble. X-ray diffraction data were indexed and integrated using the program MOSFLM and scaled with the program SCALA (20). An initial MAD density map was generated by locating four selenium sites in the data sets Peak, Inflection, High-energy remote using the program SOLVE (21), which was also used to calculate phases. RESOLVE (22) was used for solvent flattening, assuming a 40% solvent content. The structure was built using MAIN (23) and refined using CNS (24). The structure of the native protein was determined by molecular replacement using the program CNS. The PUB domain in which Asn-41, Lys-50, and Tyr-51 are replaced with alanine was crystallized in 50% polyethylene glycol 400, 0.1 M CHES, pH 9.5, 0.2 M NaCl. The structure of the mutant protein was determined by molecular replacement using the structure of the native protein.

p97 Binding Assay—Plasmids for the bacterial expression of amino-terminal glutathione S-transferase (GST) fusions of human PNGase (residues 11-109; GST-PUBPNGase) and human UBXD1 (residues 150-264; GST-PUBUBXD1) and of amino-terminal hexahistidine fusions of full-length human p97 and p47 were cloned according to standard procedures. Plasmid pProExHT-p97{Delta}N for the expression of hexahistidine-tagged human p97 lacking residues 1-199 (p97{Delta}N) was a kind gift from P. Zwickl (Martinsried, Germany). Plasmid pQE30-FAF1 (25) for the expression of hexahistidine-tagged full-length human FAF1 was a kind gift from O. G. Issinger (Odense, Denmark). Site-directed mutagenesis of GST-PUBPNGase was performed using the QuikChange II XL kit (Stratagene) according to the manufacturer's instructions. All proteins were purified by glutathione or nickel-nitrilotriacetic acid affinity chromatography as applicable according to standard procedures. Glutathione-Sepharose pull-downs were performed exactly as described (26), using 10 µl of beads, 40 µg (1.5 nmol) of GST, 60 µg (1.6 nmol) of GST-PUB domain fusions, 20 µg (0.22 nmol) of His6-p97, 15 µg (0.2 nmol) of His6-p97{Delta}N, 100 µg (2.4 nmol) of His6-p47, and 150 µg (2.0 nmol) of His6-FAF1. In the experiment described in Fig. 5E, 40 µl of beads, 200 µg of GST (7.7 nmol), and 60 µg (1.6 nmol), 120 µg (3.2 nmol), or 300 µg (8 nmol) of GST-PUB domain fusion was used. In the experiments described in Fig. 5, D and E, His6-p97 and His6-p47 or His6-FAF1 were preincubated for 1 h at 4 °C before the preincubation mixture was added to GST-PUBPNGase immobilized on glutathione-Sepharose beads. The protein concentrations in the preincubation mixture were: 2.4 µM His6-p97, 28.6 µM His6-p47, and 21.3 µM His6-FAF1.


Figure 1
View larger version (17K):
[in this window]
[in a new window]
  		FIGURE 1.
Domain architectures of PUB domain-containing proteins. Selected proteins from man (Hs), Arabidopsis thaliana (At), Cryptosporidium parvum (Cp), and Plasmodium falciparum (Pf) with links to the ubiquitin-proteasome system are shown. Domains are labeled according to the SMART data base. WLM and PPDE denote putative deubiquitinating enzyme domains (36). The proteins were either previously reported to contain the domain (15) or were identified in sequence similarity searches with the program psi-blast (www.ncbi.nlm.nih.gov/BLAST) using the amino acid sequence of the amino-terminal domain of PNGase as a query.

 

   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
DISCUSSION
 REFERENCES
 
PUB Domain Structure—The amino-terminal PUB domain of human PNGase could be easily expressed in E. coli, and the purified protein produced crystals that diffracted well. As the domain contains only one methionine residue we prepared a L66M/L75M/L87M mutant protein to facilitate the incorporation of sufficient amounts of seleno-methionine to enable the structure of the domain to be solved using the multi-wavelength anomalous dispersion method. The seleno-methionine-substituted mutant protein readily crystallized, and its structure was determined using multiwavelength anomalous dispersion. The structure of the native domain was then determined to 1.6 Å by molecular replacement using the mutant structure. Crystallographic data are summarized in Table 1. A representative section of the electron density is shown in Fig. 2A. The PUB domain fold consists of a bundle of five {alpha} helices that pack onto a short three-stranded anti-parallel beta sheet (Fig. 2B). In addition, there is a small 310 helix in the loop between the second helix and the first beta strand. The structure has some resemblance to the wing helix motif, which also consists of helices packing onto a small beta sheet. The topological arrangement of the helices is, however, very different, and a search of the protein structure data base using the program DALI (27) revealed no significant similarity to other known structures. A structure-based alignment of a selection of PUB domains indicates that insertions and deletions are restricted to loops between elements of secondary structure (Fig. 3) and are unlikely to affect the overall fold. Thus, the structure of the PNGase PUB domain can serve as a good model for other PUB domains.


View this table:
[in this window]
[in a new window]
  		TABLE 1
Crystallographic data The first two columns refer to the Se derivative, and the third column refers to the native protein.

 


Figure 2
View larger version (26K):
[in this window]
[in a new window]
  		FIGURE 2.
Crystal structure of the PUB domain of human PNGase. A, electron density from the 1.6 Å map. B, overall structure of the PUB domain. The ribbon representation is color coded from the amino terminus (dark blue) to the carboxyl terminus (dark red), and secondary structure elements are labeled (prepared using the program Pymol (www.pymol.org).

 
A number of residues are highly conserved in PUB domains (Fig. 3). Gly-79 and Phe-80 are located in the turn between the fourth {alpha} helix and the second strand of the sheet. Both residues have a structural role: a glycine residue is required for the sharp turn at the end of the helix, and the side chain of the phenylalanine residue packs into the hydrophobic core of the domain. Similarly, the highly conserved residue Leu-35 is at the center of the hydrophobic core of the domain. In contrast, several hydrophilic conserved residues are exposed at the protein surface. Asn-41, which is at the end of the second helix, is conserved in all PUB domains, and Asn-58 in helix 3 is highly conserved. Lys-50 and Tyr-51, both of which are in the 310 helix, Glu-73 in the third {alpha} helix, and Arg-55 in the first beta strand are also highly conserved and mostly replaced conservatively in other PUB domains. Intriguingly, the side chains of most of these residues line a basic pocket on the protein surface, and residues Asn-41, Lys-50, and Tyr-51 form a well defined surface patch (Fig. 4). The clustering of three highly conserved residues in this surface patch strongly suggests that it is a functionally important site.

p97 Binding—Recently, p97 has been shown to interact with human PNGase in vivo and in vitro (17, 19). To test whether the PUB domain is involved in p97 binding, we performed an in vitro GST pulldown experiment using the isolated PUB domain of human PNGase (Fig. 5A). Indeed, the isolated PUB domain bound p97 very efficiently (compare lane 4 to the input shown in lane 1), whereas no background binding of p97 to GST alone was detectable (lane 2). Similar results were obtained with the PUB domain of human UBXD1 protein (lane 3) (28). These data show for the first time the direct binding of PUB domains to p97. Furthermore, they suggest that the PUB domain is a novel p97 binding module.


Figure 3
View larger version (20K):
[in this window]
[in a new window]
  		FIGURE 3.
Structure-based sequence alignment of PUB domains. The residue numbers of the PUB domains shown in Fig. 1 are given next to the protein names. Secondary structure elements of the PNGase PUB domain are indicated at the top. The most highly conserved residues are boxed. Asterisks mark three highly conserved residues forming a solvent-exposed surface patch (see Fig. 4). The alignment was prepared using the program Jalview (37).

 


Figure 4
View larger version (56K):
[in this window]
[in a new window]
  		FIGURE 4.
A conserved surface patch on the PUB domain. A, ribbon representation of the PNGase PUB domain with conserved, solvent-exposed residues shown as sticks. B, surface electrostatic potential shown in the same orientation.

 
We next analyzed the importance of the conserved surface patch consisting of residues Asn-41, Lys-50, and Tyr-51 for p97 binding, using a site-directed mutagenesis approach. Altering residues Asn-41 and Lys-50 of the PUB domain of human PNGase to alanine, either alone or in combination, did not result in a significant decrease in p97 binding as compared with the wild-type PUB domain (Fig. 5A, lanes 4-7). The combined mutation of residues Lys-50 and Tyr-51 led only to a slight reduction in p97 binding (lane 8). In contrast, mutation of all three conserved residues almost completely abolished binding of p97 to the mutant PUB domain (lane 9). To verify that the three amino acid exchanges did not affect the overall protein structure, we determined the crystal structure of the NKY41,50,51AAA mutant PUB domain. The structure of the triple mutant PUB domain is identical to that of the native protein, and the C{alpha} atoms can be superimposed with a root mean square deviation of 0.17 Å (Fig. 5B). The lack of binding activity of the mutated domain is therefore solely the result of the removal of functionally important side chains, indicating that the conserved surface patch comprising residues Asn-41, Lys-50, and Tyr-51 forms part of the major p97 binding site on the PUB domain.

Many p97 cofactors, including the heterodimer Ufd1/Npl4 and members of the large family of UBX domain-containing proteins, bind to the amino-terminal N domain of p97 (29-31). We therefore tested in NMR chemical shift-mapping experiments whether the PUB domain of human PNGase similarly binds to the N domain of p97. However, unlike the p47 UBX domain (32), the PUB domain did not show detectable binding to the isolated N domain of p97 (data not shown). To test whether the PUB domain binds to the D1 and/or D2 ATPase domains of p97, we repeated the pulldown experiment with a truncated p97 variant lacking the N domain, p97{Delta}N. p97{Delta}N bound efficiently and specifically to the PUB domains of PNGase and UBXD1 (Fig. 5C), showing that the PUB domain binding site resides within the D1 and/or D2 ATPase domains of p97.

The distinct binding regions of p97 for the PUB versus UBX domains raised the possibility that both domains can bind simultaneously to p97. Indeed, incubation of p97 with a 10-fold molar excess of the UBX domain proteins p47 or FAF1 did not prevent its binding to the PUB domain in a GST pulldown experiment (Fig. 5D, lanes 7-9), suggesting that binding of UBX and PUB domains is not mutually exclusive. Consistent with this interpretation, significant binding of p47 and FAF1 to the GST-PUB domain fusion was observed in the presence, but not the absence, of p97 (compare lanes 8 and 9 with lanes 10 and 11). To ensure that the amount of PUB domain used in this experiment was saturating with respect to p97, we performed a titration experiment with increasing amounts of GST-PUB domain fusion protein (Fig. 5E). We found that the lowest PUB domain concentration, which had been used in the previous experiments, was already saturating, because no additional p97 bound to a 5-fold higher concentration (lanes 3-5). Consistently, the amount of p47 bound to p97 remained constant even at the highest concentration of PUB domain used (lanes 6-8), whereas no p47 bound to the PUB domain in the absence of p97 (lane 9). Taken together, these data demonstrate that binding of the PUB domain and of UBX domain proteins to p97 is not mutually exclusive.


Figure 5
View larger version (38K):
[in this window]
[in a new window]
  		FIGURE 5.
The PUB domain is a novel p97 binding module. A, p97 and the PUB domain interact directly via the conserved surface patch. GST fusion proteins of the PUB domain of human UBXD1 (GST-PUBUBXD1; lane 3) or wild-type or the indicated mutant PUB domains from human PNGase (GST-PUBPNGase; lanes 4-9) bound to glutathione beads were incubated with p97. After repeated washing steps, binding of p97 was analyzed by SDS-PAGE followed by Coomassie staining of the gel. GST served as negative control (lane 2). For comparison, 10% of the p97 input is shown (lane 1). B, the NKY41,50,51AAA triple mutant PUB domain has the same structure as the wild-type domain. Stereo view of the superimposed backbone traces of the wild-type (red) and mutant (blue) PUB domains. C, the PUB domain binds to the D1 and/or D2 ATPase domains of p97. Binding of p97 or p97{Delta}N as indicated to the PUB domains of human PNGase or human UBXD1 was tested as in panel A. D, binding of the PUB domain and UBX domain proteins is not mutually exclusive. Binding of p97 to the PUB domain of human PNGase was tested as in panel A after preincubating p97 without (lane 7) or with a 10-fold molar excess of the UBX domain proteins p47 (lane 8) or FAF1 (lane 9). E, p47 binds to p97 in the presence of saturating amounts of PUB domain. Binding of p97 and p47 to the PUB domain of human PNGase was tested as in panel D in the presence of increasing amounts of PUB domain. In lanes 3 and 6, the same amount of PUB domain as in panels A, C, and D was used. In lanes 4 and 7, twice the amount of PUB domain was; in lanes 2, 5, 8, and 9, the 5-fold amount of GST and PUB domain, respectively, was used.

 

   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
REFERENCES
 
p97 is a chaperone-like molecular machine involved in a variety of cellular functions, including protein degradation, membrane fusion, and cell cycle regulation (31, 33). This remarkable versatility of p97 depends critically on its ability to form complexes with a number of different cofactors mediating distinct activities (31). Substrate-recruiting cofactors including the heterodimer Ufd1/Npl4 and members of the large UBX protein family regulate the substrate specificity of p97 (26, 34), while substrate-processing cofactors like the ubiquitin chain elongation enzyme Ufd2 or the deubiquitinylating enzymes VCIP135 and Otu1 determine the fate of p97 substrates (31, 35). In addition, a significant number of p97-binding proteins exist whose mechanism of action on p97 is still poorly characterized, among them PNGase (17).

In a structural approach to investigate p97 cofactor functions, we solved the three-dimensional structure of the PUB domain of human PNGase. Although the structure did not possess significant similarities to known proteins, it revealed a conserved surface patch suggestive of a functionally important site (Fig. 4). Indeed, our biochemical analysis showed that the PUB domain is a novel p97 binding module and that the conserved surface patch is a major p97 binding site (Fig. 5A). Interestingly, the PUB domain does not bind to the N domain of p97, unlike the well established substrate-recruiting cofactors Ufd1/Npl4 and p47, but rather to the D1 and/or D2 ATPase domains. Consequently, binding of the PUB domain is not mutually exclusive with binding of substrate-recruiting UBX proteins (Figs. 5, D and E). The use of a different binding site on p97 by the PUB domain could simply allow the assembly of larger complexes on p97. Alternatively, the interaction with the D1 and/or D2 domains may enable the PUB domain to coordinate the ATP hydrolysis-dependent substrate-extracting activity of p97 with PNGase-catalyzed deglycosylation and substrate-processing activities of other cofactors, perhaps including the proteasomal targeting factor HR23B (9, 17).

Our finding that the two distantly related PUB domains of human PNGase and human UBXD1 both bind to p97 (Fig. 5A) suggests strongly that PUB domain-containing proteins constitute a novel family of p97 cofactors. PUB domain proteins are found in the plant and animal kingdoms and are particularly common in trypanosomes, which contain several unique families of PUB domain-containing proteins (36). The PUB domain is often found in proteins that also contain domains associated with ubiquitin conjugation and removal (Fig. 1). It is tempting to speculate that these proteins coordinate assembly and/or processing of ubiquitin chains with p97 function.

UBXD1-like proteins containing both PUB and UBX domains together are evolutionary conserved and represent the most widespread group of PUB domain proteins (28). Although none of these proteins has been characterized in any detail, the simultaneous presence of two p97 binding domains suggests that UBXD1-like proteins may be important regulators of p97 function. Intriguingly, the major p97 binding motif found in most UBX domains (residues FPR in the turn between strands 3 and 4 of the UBX domain) (29, 32) is significantly altered in UBXD1-like proteins (28, 29), thus potentially decreasing the affinity for p97. We speculate that the presence of a second p97 binding module (the PUB domain) within UBXD1-like proteins allowed for the modulation of the binding properties of the UBX domain during evolution. It will be interesting to study the details of such a dual p97 binding mechanism. The identification of PUB domain proteins as an entire new family of potential p97 cofactors further emphasizes the key role of substrate-recruiting and substrate-processing proteins for the regulation of the p97 molecular machine and opens a new direction for the investigation of p97 functions in various organisms.


   FOOTNOTES
 
* This work was supported by Emmy Noether Grant Bu 951/1-1 to /1-4 of the Deutsche Forschungsgemeinschaft (to A. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

1 To whom correspondence may be addressed. Tel.: 49-89-85783050; Fax: 49-89-85783055; E-mail: buchberg{at}biochem.mpg.de. 2 To whom correspondence may be addressed. Tel.: 44-1223-402129; Fax: 44-1223-402140; E-mail: mb10031{at}cus.cam.ac.uk.

3 The abbreviations used are: PNGase, peptide N-glycanase; GST, glutathione S-transferase; CHES, 2-(cyclohexylamino)ethanesulfonic acid. Back


   ACKNOWLEDGMENTS
 
We thank Sandra Köglsberger for excellent technical assistance, Peter Zwickl for providing the p97{Delta}N expression plasmid, Olaf-Georg Issinger for providing the FAF1 expression plasmid, and Roger Williams for help with data collection.



   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 

  1. Romisch, K. (2005) Annu. Rev. Cell Dev. Biol. 21, 435-456[CrossRef][Medline] [Order article via Infotrieve]
  2. Meusser, B., Hirsch, C., Jarosch, E., and Sommer, T. (2005) Nat. Cell Biol. 7, 766-772[CrossRef][Medline] [Order article via Infotrieve]
  3. Bays, N. W., and Hampton, R. Y. (2002) Curr. Biol. 12, R366-R371[CrossRef][Medline] [Order article via Infotrieve]
  4. DeLaBarre, B., and Brunger, A. T. (2003) Nat. Struct. Biol. 10, 856-863[CrossRef][Medline] [Order article via Infotrieve]
  5. DeLaBarre, B., and Brunger, A. T. (2005) J. Mol. Biol. 347, 437-452[CrossRef][Medline] [Order article via Infotrieve]
  6. Beuron, F., Dreveny, I., Yuan, X., Pye, V. E., McKeown, C., Briggs, L. C., Cliff, M. J., Kaneko, Y., Wallis, R., Isaacson, R. L., Ladbury, J. E., Matthews, S. J., Kondo, H., Zhang, X., and Freemont, P. S. (2006) EMBO J.
  7. Watts, G. D., Wymer, J., Kovach, M. J., Mehta, S. G., Mumm, S., Darvish, D., Pestronk, A., Whyte, M. P., and Kimonis, V. E. (2004) Nat. Genet. 36, 377-381[CrossRef][Medline] [Order article via Infotrieve]
  8. Weihl, C. C., Dalal, S., Pestronk, A., and Hanson, P. I. (2006) Hum. Mol. Genet. 15, 189-199[Abstract/Free Full Text]
  9. Katiyar, S., Li, G., and Lennarz, W. J. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 13774-13779[Abstract/Free Full Text]
  10. Blom, D., Hirsch, C., Stern, P., Tortorella, D., and Ploegh, H. L. (2004) EMBO J. 23, 650-658[CrossRef][Medline] [Order article via Infotrieve]
  11. Kim, I., Ahn, J., Liu, C., Tanabe, K., Apodaca, J., Suzuki, T., and Rao, H. (2006) J. Cell Biol. 172, 211-219[Abstract/Free Full Text]
  12. Lee, J. H., Choi, J. M., Lee, C., Yi, K. J., and Cho, Y. (2005) Proc. Natl. Acad. Sci. U. S. A. 102, 9144-9149[Abstract/Free Full Text]
  13. Park, H., Suzuki, T., and Lennarz, W. J. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 11163-11168[Abstract/Free Full Text]
  14. Zhao, G., Zhou, X., Wang, L., Li, G., Kisker, C., Lennarz, W. J., and Schindelin, H. (2006) J. Biol. Chem. 281, 13751-13761[Abstract/Free Full Text]
  15. Suzuki, T., Park, H., Till, E. A., and Lennarz, W. J. (2001) Biochem. Biophys. Res. Comm. 287, 1083-1087[CrossRef][Medline] [Order article via Infotrieve]
  16. Doerks, T., Copley, R. R., Schultz, J., Ponting, C. P., and Bork, P. (2002) Genome Res. 12, 47-56[Abstract/Free Full Text]
  17. Li, G., Zhou, X., Zhao, G., Schindelin, H., and Lennarz, W. J. (2005) Proc. Natl. Acad. Sci. U. S. A. 102, 15809-15814[Abstract/Free Full Text]
  18. Katiyar, S., Joshi, S., and Lennarz, W. J. (2005) Mol. Biol. Cell 16, 4584-4594[Abstract/Free Full Text]
  19. McNeill, H., Knebel, A., Arthur, J. S., Cuenda, A., and Cohen, P. (2004) Biochem. J. 384, 391-400[CrossRef][Medline] [Order article via Infotrieve]
  20. Leslie, A. G. W. (1991) CCP4 and ESF-EACMB Newsletter on Protein Crystallography, SERC Laboratory, Daresbury, Warrington
  21. Terwilliger, T. C., and Berendzen, J. (1999) Acta Crystallogr. Sect. D Biol. Crystallogr. 55, 849-861[CrossRef][Medline] [Order article via Infotrieve]
  22. Terwilliger, T. C. (2000) Acta Crystallogr. Sect. D Biol. Crystallogr. 56, 965-972[CrossRef][Medline] [Order article via Infotrieve]
  23. Turk, D. (1992) Further Development of a Program for Molecular Graphics and Electron Density Manipulation and Its Application to Protein Structure Determination. Ph.D. thesis, Technische Universität, München
  24. Brunger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921[CrossRef][Medline] [Order article via Infotrieve]
  25. Olsen, B. B., Jessen, V., Hojrup, P., Issinger, O. G., and Boldyreff, B. (2003) FEBS Lett. 546, 218-222[CrossRef][Medline] [Order article via Infotrieve]
  26. Schuberth, C., Richly, H., Rumpf, S., and Buchberger, A. (2004) EMBO Rep. 5, 818-824[CrossRef][Medline] [Order article via Infotrieve]
  27. Holm, L., and Sander, C. (1995) Trends Biochem. Sci. 20, 478-480[CrossRef][Medline] [Order article via Infotrieve]
  28. Carim-Todd, L., Escarceller, M., Estivill, X., and Sumoy, L. (2001) Biochim. Biophys. Acta 1517, 298-301[Medline] [Order article via Infotrieve]
  29. Buchberger, A., Howard, M. J., Proctor, M., and Bycroft, M. (2001) J. Mol. Biol. 307, 17-24[CrossRef][Medline] [Order article via Infotrieve]
  30. Yuan, X., Shaw, A., Zhang, X., Kondo, H., Lally, J., Freemont, P. S., and Matthews, S. (2001) J. Mol. Biol. 311, 255-263[CrossRef][Medline] [Order article via Infotrieve]
  31. Dreveny, I., Pye, V. E., Beuron, F., Briggs, L. C., Isaacson, R. L., Matthews, S. J., McKeown, C., Yuan, X., Zhang, X., and Freemont, P. S. (2004) Biochem. Soc. Trans. 32, 715-720[CrossRef][Medline] [Order article via Infotrieve]
  32. Dreveny, I., Kondo, H., Uchiyama, K., Shaw, A., Zhang, X., and Freemont, P. S. (2004) EMBO J. 23, 1030-1039[CrossRef][Medline] [Order article via Infotrieve]
  33. Woodman, P. G. (2003) J. Cell Sci. 116, 4283-4290[Abstract/Free Full Text]
  34. Meyer, H. H., Shorter, J. G., Seemann, J., Pappin, D., and Warren, G. (2000) EMBO J. 19, 2181-2192[CrossRef][Medline] [Order article via Infotrieve]
  35. Rumpf, S., and Jentsch, S. (2006) Mol. Cell 21, 261-269[CrossRef][Medline] [Order article via Infotrieve]
  36. Iyer, L. M., Koonin, E. V., and Aravind, L. (2004) Cell Cycle 3, 1440-1450[Medline] [Order article via Infotrieve]
  37. Clamp, M., Cuff, J., Searle, S. M., and Barton, G. J. (2004) Bioinformatics 20, 426-427[Abstract/Free Full Text]

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
I. Shapira, D. Charuvi, Y. Elkabetz, K. Hirschberg, and S. Bar-Nun
Distinguishing between retention signals and degrons acting in ERAD
J. Cell Sci., December 15, 2007; 120(24): 4377 - 4387.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. Kato and Y. Kamiya
Structural views of glycoprotein-fate determination in cells
Glycobiology, October 1, 2007; 17(10): 1031 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Zhao, X. Zhou, L. Wang, G. Li, H. Schindelin, and W. J. Lennarz
Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation
PNAS, May 22, 2007; 104(21): 8785 - 8790.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Park, D. M. Rancour, and S. Y. Bednarek
Protein Domain-Domain Interactions and Requirements for the Negative Regulation of Arabidopsis CDC48/p97 by the Plant Ubiquitin Regulatory X (UBX) Domain-containing Protein, PUX1
J. Biol. Chem., February 23, 2007; 282(8): 5217 - 5224.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/35/25502    most recent
M601173200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, M. D.
Right arrow Articles by Bycroft, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, M. D.
Right arrow Articles by Bycroft, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.