Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M605965200 on August 22, 2006

J. Biol. Chem., Vol. 281, Issue 43, 32148-32155, October 27, 2006
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/43/32148    most recent
M605965200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Tandon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Tandon, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Cytosolic Proteins Regulate {alpha}-Synuclein Dissociation from Presynaptic Membranes*

Sabine Wislet-Gendebien1, Cheryl D'Souza, Toshitaka Kawarai, Peter St George-Hyslop, David Westaway, Paul Fraser, and Anurag Tandon2

From the Centre for Research in Neurodegenerative Diseases, University of Toronto, Toronto, Ontario M5S 3H2, Canada

Received for publication, June 22, 2006 , and in revised form, August 3, 2006.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
Intracellular accumulation of insoluble {alpha}-synuclein in Lewy bodies is a key neuropathological trait of Parkinson disease (PD). Neither the normal function of {alpha}-synuclein nor the biochemical mechanisms that cause its deposition are understood, although both are likely influenced by the interaction of {alpha}-synuclein with vesicular membranes, either for a physiological role in vesicular trafficking or as a pathological seeding mechanism that exacerbates the propensity of {alpha}-synuclein to self-assemble into fibrils. In addition to the {alpha}-helical form that is peripherally-attached to vesicles, a substantial portion of {alpha}-synuclein is freely diffusible in the cytoplasm. The mechanisms controlling {alpha}-synuclein exchange between these compartments are unknown and the possibility that chronic dysregulation of membrane-bound and soluble {alpha}-synuclein pools may contribute to Lewy body pathology led us to search for cellular factors that can regulate {alpha}-synuclein membrane interactions. Here we reveal that dissociation of membrane-bound {alpha}-synuclein is dependent on brain-specific cytosolic proteins and insensitive to calcium or metabolic energy. Two PD-linked mutations (A30P and A53T) significantly increase the cytosol-dependent {alpha}-synuclein off-rate but have no effect on cytosol-independent dissociation. These results reveal a novel mechanism by which cytosolic brain proteins modulate {alpha}-synuclein interactions with intracellular membranes. Importantly, our finding that {alpha}-synuclein dissociation is up-regulated by both familial PD mutations implicates cytosolic cofactors in disease pathogenesis and as molecular targets to influence {alpha}-synuclein aggregation.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 
{alpha}-Synuclein ({alpha}-syn)3 is a member of a multigene synuclein family that is highly abundant in presynaptic terminals of mammalian brain (1, 2). The function of {alpha}-syn is poorly resolved, although it is attributed with wide ranging roles in vesicular trafficking and vesicle biogenesis and as a molecular chaperone (3). {alpha}-Syn is also implicated in a broad spectrum of neurodegenerative disorders collectively named synucleinopathies, being a primary component of Lewy bodies (4), and as a fragment in Alzheimer disease plaques (5). In a small number of pedigrees, autosomal-dominant inheritance of Parkinson disease (PD) is linked to either multiplication of the normal {alpha}-syn gene or to one of three missense mutations (A30P, E46K, and A53T) (6, 7).

In vitro studies suggest that {alpha}-syn is natively unfolded in aqueous solution, and exposure to lipids stabilizes the amino terminus in an amphipathic {alpha}-helix that aligns polar and non-polar residues into opposing orientations (8–11). Presumably, this secondary structure confers the lipid-binding properties for direct membrane interaction such that purified recombinant {alpha}-syn can bind to small diameter artificial vesicles rich in acidic phospholipids (8, 12), to purified synaptic vesicles (13, 14), and to membranes within intact cells (15). Most studies examining {alpha}-syn membrane binding have used either artificial phospholipids or purified membranes, without consideration to the potential regulatory function of soluble or membrane factors that may be pertinent to disease progression. Previous investigations were equivocal on whether {alpha}-syn mutations (A30P and A53T) affect membrane interactions; the A30P or A53T mutation had little or no effect on {alpha}-syn binding (16, 17), the A53T mutation reduced (8) or increased (18, 19) the membrane binding, or the A30P had reduced binding to purified synaptic vesicles (14, 20, 21). Further complexity may also arise from reversible membrane interaction, akin to that of cytosolic regulators of vesicular trafficking whose direct or indirect association with vesicle membranes is necessarily transient (e.g. exocytosis: NSF, {alpha}/beta-SNAP, rab3, rabphilin, synapsin, CAPS; endocytosis: clathrin/adaptor, dynamin) (22–24). Association and dissociation is coupled to the vesicle life cycle, dictated by cation fluxes, nucleotide triphosphate cleavage, and post-translational modifications. In the case of rab3, an additional mechanism governs its membrane attachment. Another cytosolic protein, GDP-dissociation inhibitor, is essential to extract rab3 off exocytosing vesicles and deliver it to newly synthesized vesicles (25–27).

It is unknown whether {alpha}-synuclein exchange occurs between soluble and membrane compartments or even whether {alpha}-syn can dissociate from reconstituted or biological membranes. The possibility that chronic dysregulation of subcellular {alpha}-synuclein pools may contribute to PD and other synucleinopathies prompted our search for cellular factors that affect {alpha}-synuclein membrane interactions. To address this, we developed an assay comprised of permeabilized synaptosomes and report here that {alpha}-syn dissociation from presynaptic membranes requires cytosolic proteins from the brain. Particularly relevant to disease pathogenesis was the finding that cytosol-dependent {alpha}-syn dissociation was significantly increased by two PD-related mutations (A30P and A53T) suggesting a mechanism whereby cytosolic cofactors can regulate {alpha}-syn solubility and, thereby, modulate disease progression.


Figure 1
View larger version (49K):
[in this window]
[in a new window]
  		FIGURE 1.
A, {alpha}-syn dissociation assay. Step 1, synaptosomes and cytosol were prepared separately from brains of Tg mice expressing the human {alpha}-syn (Wt, A30P, or A53T) and {alpha}-syn-deficient mice, respectively. Step 2, following hypotonic lysis of synaptosomes, the washed membranes ({alpha}-syn donor) were co-incubated with {alpha}-syn–/– cytosol ({alpha}-syn acceptor). Step 3, membrane and cytosolic fractions were separated by centrifugation and the supernatants were analyzed by Western blotting. B, distribution of {alpha}-syn in acceptor and donor fractions. Western blots showing total synaptosomal {alpha}-syn (detected with anti-{alpha}-syn antibody Syn-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and synaptophysin recovered in either supernatant (S) or pellet (P) following resuspension of intact synaptosomes in isotonic buffer, hypotonic buffer, or hypotonically lysed synaptosomes (Donor fraction) washed three times and resuspended in isotonic buffer for 10, 20, or 30 min. As expected, no {alpha}-syn was detected in brain cytosol from {alpha}-syn-deficient mice (acceptor fraction: knockout cytosol).

 

   EXPERIMENTAL PROCEDURES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
RESULTS
 DISCUSSION
 REFERENCES
 
Transgene Construction and Generation of Transgenic Mice—The human {alpha}-syn gene containing a eukaryotic Kozak initiation sequence (GCCGCCACC) (28, 29) upstream of the start codon was ligated into a linearized cos-Tet expression vector containing the Syrian hamster prion protein promoter gene (30, 31). A30P and A53T {alpha}-syn mutant transgenes were generated using the Quick change kit (Stratagene). Following microinjection into fertilized oocytes of FVB/N mice, positive founders were identified by amplification of genomic DNA and bred to FVB wild-type mice. To generate {alpha}-syn Tg mice lacking endogenous murine {alpha}-syn, the {alpha}-syn Tg mice were crossed with SNCA–/– mice (Jackson Laboratories). F1 off-spring positive for {alpha}-syn transgene were backcrossed and F2 progeny were selected for the presence of the neomycin resistance gene and the absence of the endogenous {alpha}-syn gene as described previously (32). All animal experiments were performed according to guidelines established in the Canadian Guide for the Care and Use of Laboratory Animals.

Synaptosome Preparation—Synaptosomes were prepared as described (26, 33). Briefly, brains from mice (A30P, A53T, and Wt transgenic mice) were dissected and homogenized with 10 strokes at 500 rpm, in ice-cold buffer A (320 mM sucrose, 1 mM EGTA, and 5 mM HEPES (pH 7.4) and centrifuged at 3000 x g for 10 min. The supernatant was spun for 10 min at 14,000 x g and the pellet (P2) resuspended in buffer A. The P2 fraction was loaded onto a discontinuous Ficoll gradient (13, 9, and 5% in buffer A) and centrifuged for 35 min at 35,000 x g. The 13–9% interface, containing intact synaptosomes, was resuspended in buffer B (140 mM NaCl, 5 mM KCl, 20 mM HEPES, 5 mM NaHCO3, 1.2 mM Na2HPO4, 1 mM MgCl2, 1 mM EGTA, and 10 mM glucose) and spun at 14,000 x g for 10 min. The pellet was hypotonically lysed by two washes in buffer C (10 mM HEPES, 18 mM KOAc (pH 7.2)), spun at 14,000 x g for 10 min, and resuspended in buffer D (25 mM HEPES, 125 mM KOAc, and 2.5 mM MgCl2). After centrifugation (14,000 x g for 10 min), synaptosomes were resuspended in buffer D and incubated (as indicated in each figure legend) with or without {alpha}-syn deficient brain or liver cytosol before separating membrane and supernatant by centrifugation at 14,000 x g for 10 min. The supernatant was centrifuged at 100,000 x g to remove trace contaminating elements such as broken membranes or dissociated synaptic vesicles before quantifying soluble {alpha}-syn by Western blotting.

Cytosol Preparation from {alpha}-Syn Knock-out and Non-transgenic Mice—Mouse brains were homogenized in 10 volumes of homogenizing buffer (1 mM sucrose, 1 M KOAc, 100 mM MgOAc, 1 M HEPES (pH 7.4)). The homogenate was spun at 15,000 x g for 10 min and the supernatant was then centrifuged at 100,000 x g for 1 h. The supernatant was dialyzed 4 times with cold dialysis buffer (1 mM KOAc, 1 M HEPES (pH 7.2)) for 45 min each, centrifuged at 28,000 x g for 25 min, and stored at –80 °C. Protein concentration was determined by BCA protein assay (Pierce).


Figure 2
View larger version (44K):
[in this window]
[in a new window]
  		FIGURE 2.
A, cytosol-dependent {alpha}-syn dissociation from synaptosome membranes. {alpha}-Syn Tg membranes and brain {alpha}-syn-deficient cytosol were co-incubated to assess the role of cytosolic proteins on {alpha}-syn dissociation. Incubation for 10 min at 37 °C of hypotonically lysed synaptosome membranes (100 mg of wet synaptosomal membrane weight) with various cytosol concentrations (0–1.5 mg/ml, total protein concentration) increased soluble human {alpha}-syn, as detected by human-specific antibody 211. The integral membrane protein synaptophysin was not released into the supernatant but remained associated with the membrane pellet (Membr) solubilized with 1% Triton X-100 buffer. B, quantification of cytosol concentration on dissociation of human {alpha}-syn. Mean {alpha}-syn intensity and S.E. of four independent experiments and normalized to {alpha}-syn solubilized by 1.5 mg/ml cytosol. {alpha}-Syn dissociation in the presence of 1.5 mg/ml cytosol was blocked by pretreatment of membranes with LWS1 {alpha}-syn antibody (Student's t test, p < 0.0001, n = 4, compared with 1.5 mg/ml cytosol without antibody condition), but not anti-synaptophysin antibody (Student's t test, p > 0.3, n = 4) or preimmune serum (Student's t test, p > 0.7, n = 4). C, regulated dissociation of murine {alpha}-syn. Western blot probed with Syn-1 antibody showing endogenous murine {alpha}-syn from non-Tg mice also exhibited cytosol-dependent dissociation from synaptosome membranes upon incubation for 10 min at 37 °C with 1.5 mg/ml {alpha}-syn–/– cytosol.

 
Western Blotting—Proteins were boiled briefly in loading buffer (10% v/v glycerol; 0.05 M Tris (pH 6.8), 2% SDS, bromphenol blue, and 2.5% v/v beta-mercaptoethanol) and separated by electrophoresis using 12% Tris-glycine polyacrylamide gels. Proteins were transferred to nitrocellulose (Life Sciences) and probed by Western blotting using: antibodies against {alpha}-syn (monoclonals 211 and Syn-1, Neomarkers), our own rabbit polyclonal (LWS1, 1:1000) raised to a 24-mer {alpha}-syn-specific peptide, glyceraldehyde-3-phosphate dehydrogenase (monoclonal 6C5, Biodesign), or synaptophysin (monoclonal, Biodesign). Bound horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Sigma) were revealed by chemiluminescence using ECL Plus (GE Healthcare) and quantified with a Storm 860 fluorescent imager and ImageQuant software (GE Healthcare). Statistical comparisons were done with Graph-Pad InStat software using Student's t test for comparisons between two groups or ANOVA (Bonferroni's test) for multiple comparisons.


   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
DISCUSSION
 REFERENCES
 
Cytosolic Proteins Increase {alpha}-Syn Dissociation from Membrane—To identify regulators of {alpha}-syn solubility, we assessed the effects of cytosolic proteins on the interaction of {alpha}-syn with membranes. We measured {alpha}-syn dissociation from synaptosomal membranes prepared from brains of transgenic mice expressing human {alpha}-syn as the donor of membrane-bound {alpha}-syn in the absence or presence of brain cytosol derived from {alpha}-syn-deficient mice as the acceptor soluble fraction (Fig. 1A). Intact synaptosomes from {alpha}-syn transgenic mouse brain suspended in hypotonic buffer release ~30% of their total {alpha}-syn content, which comprises the soluble cytoplasmic pool (Fig. 1B). The remaining {alpha}-syn is stably associated with the membrane fraction following resuspension in isotonic buffer and is entirely susceptible to membrane disruption by detergents. The release of membrane-derived {alpha}-syn into the supernatant was markedly increased by the addition of cytosol, whereas integral membrane proteins, such as synaptophysin, were retained by the membrane pellet (Fig. 2A). Preincubation of membranes with antiserum raised to {alpha}-syn inhibited {alpha}-syn dissociation, in contrast to anti-synaptophysin antibodies or preimmune serum, neither of which had any effect (Fig. 2B). The ability to dissociate from presynaptic membranes was not exclusive to human {alpha}-syn, because endogenous {alpha}-syn from non-transgenic mice also exhibited similar cytosol-dependent dissociation from presynaptic membranes (Fig. 2C).

Comparable levels of a nonspecific protein, bovine serum albumin (BSA), did not affect {alpha}-syn solubility (Fig. 3A). However, the cytosolic activity was heat- and protease-labile as cytosol preincubated at 95 °C or pre-digested with trypsin failed to induce {alpha}-syn dissociation, suggesting that this process requires specific cytosolic proteins (Fig. 3A). To verify that proteolytic activity was completely inactivated by trypsin inhibitor before exposure to membranes, we showed that preincubation of trypsinized-cytosol with an equal concentration of untreated cytosol partially rescued {alpha}-syn dissociation activity. Moreover, equivalent concentrations of liver-derived cytosol were significantly less active compared with brain cytosol, suggesting that the factors inducing {alpha}-syn-dissociation are brainspecific (Fig. 3B). The cytosol-dependent component of {alpha}-syn dissociation from synaptic membranes was responsive to temperature, increasing ~10-fold from 4 to 37 °C. In contrast, cytosol-independent {alpha}-syn dissociation was insensitive over this range (Fig. 3C).


Figure 3
View larger version (22K):
[in this window]
[in a new window]
  		FIGURE 3.
Cytosolic brain proteins regulate {alpha}-syn dissociation from membranes. A, cytosolic activity is heat and protease labile. Increasing non-specific protein concentration with bovine serum albumin (BSA) did not induce additional {alpha}-syn dissociation compared the "no cytosol" treatment (0 mg/ml cytosol) (one-way ANOVA, p > 0.9, n = 4). Pre-exposure of brain cytosol to either heating for 3 min at 95 °C (heat-inactivated cytosol, HIC) or to trypsin (Trp) digestion completely eliminated the ability of 1.5 mg/ml cytosol (C) to induce {alpha}-syn dissociation (one-way ANOVA, p > 0.8, n = 4–8). For the trypsin experiments, proteolytic activity was terminated with trypsin inhibitor prior to the incubation with membranes, as shown by the partial rescue after half-dilution with untreated brain cytosol (Trp+C). ***, p < 0.01 relative to no cytosol condition (one way ANOVA, n = 4; Bonferroni's multiple comparison test). B, {alpha}-syn dissociation activity is concentrated in brain cytosol. The activities of brain and liver cytosol were compared. Liver-derived cytosol showed some weak activity only at the highest concentration tested, whereas brain cytosol induced significant {alpha}-syn dissociation at 6-fold lower concentration 0.5 mg/ml (***, p < 0.0001, one-way ANOVA, n = 4; Bonferroni's multiple comparison test as compared with 0 mg/ml cytosol condition). C, cytosol-dependent {alpha}-syn dissociation is influenced by temperature. Top panel, Western blot showing {alpha}-syn dissociation in presence or absence of cytosol during 10-min incubation at various temperatures (4, 16, 25, and 37 °C). Bottom panel, mean {alpha}-syn intensity and S.E. from five independent experiments and normalized to {alpha}-syn dissociation with 1.5 mg/ml cytosol (one-way ANOVA, p < 0.0001, n = 5; Bonferroni's multiple comparison test revealed that temperature significantly increased cytosol dependent {alpha}-syn dissociation). No significant correlation was observed with temperature change in absence of cytosol (one way ANOVA, p > 0.5, n = 5). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

 
Cytosolic Proteins Are Rate-limiting for {alpha}-Syn Translocation—The kinetics of {alpha}-syn dissociation from membranes are shown in Fig. 4A. Both the rate and the extent of {alpha}-syn dissociation were increased by raising the cytosol concentration. The appearance of soluble {alpha}-syn reached a plateau within 5–10 min, suggesting that cytosolic factors are rate-limiting under the conditions of our assay. Because the liberated {alpha}-syn represents less than 5% of the total membrane-associated {alpha}-syn, the majority of the {alpha}-syn is unaffected. Therefore, we examined whether any of the remaining bound {alpha}-syn was susceptible to cytosol-mediated dissociation by subsequent exposure to cytosol. Fig. 4B shows that additional membrane-bound {alpha}-syn could indeed be solubilized, although the amount in successive incubations declined somewhat, consistent with a reduction in the pool of extractable {alpha}-syn. If cytosol was omitted during the second incubation, no additional {alpha}-syn release was detected arguing that each dissociation event requires cytosolic factors and that {alpha}-syn is not primed for dissociation by the prior exposure to cytosol.

We tested whether cytosol used during one incubation retained activity to extract additional {alpha}-syn from fresh synaptic membranes. To distinguish between {alpha}-syn extracted during the first and second incubation, we used synaptosomes from non-Tg mice in the first incubation and measured the dissociation of endogenous murine {alpha}-syn using the syn-1 antibody (Fig. 4C, lane 1). The "used" cytosol, now containing murine {alpha}-syn, was incubated with permeabilized synaptosomes from human {alpha}-syn Tg mice that are murine {alpha}-syn-deficient. The anti-{alpha}-syn antibody 211, which is specific for the human isoform, did not reveal any human {alpha}-syn released from membranes in the presence of used cytosol (Fig. 4C, lane 2) but did so when using fresh cytosol (Fig. 4C, lane 3). These results suggest that the availability of cytosolic proteins determines the extent of {alpha}-syn dissociation and that they do not regenerate under our experimental conditions.


Figure 4
View larger version (34K):
[in this window]
[in a new window]
  		FIGURE 4.
Cytosolic activity is rate-limiting for{alpha}-syn dissociation. A, {alpha}-syn dissociation kinetics are regulated by cytosol concentration. Top panel, representative Western blot showing the time-course of {alpha}-syn dissociation in the absence or presence of 1.5 mg/ml brain cytosol. The extent of {alpha}-syn dissociation reached a plateau at within 5–10 min in presence of different concentrations of cytosol. Bottom panel, quantification of {alpha}-syn dissociation kinetics (mean and S.E.) from four to five independent experiments each. B, membrane {alpha}-syn is susceptible to multiple extractions with cytosol. Repeated co-incubation of the hypotonically lysed synaptosomes with fresh cytosol (+) for 10 min at 37 °C revealed a substantial extractable pool of membrane-bound {alpha}-syn. There was a slight, but statistically insignificant, decrease in {alpha}-syn dissociation with each incubation (one-way ANOVA, p > 0.2, n = 4). No {alpha}-syn was released from membranes if fresh cytosol was omitted (–), indicating the cytosolic factors must be present to induce {alpha}-syn dissociation. C, cytosolic activity is depleted by single exposure to synaptic membranes. KO cytosol was first incubated with non-Tg membranes to induce dissociation of murine {alpha}-syn as detected by monoclonal antibody Syn-1 in lane 1. The same cytosol was subsequently incubated with synaptic membranes derived from human {alpha}-syn Tg mice that lack endogenous murine {alpha}-syn. Supernatant from the second incubation in lane 2 contained only the murine {alpha}-syn from the first incubation, but no human Tg {alpha}-syn immunoreactivity was detected by the human specific {alpha}-syn monoclonal antibody 211. In contrast, fresh cytosol induced dissociation of human {alpha}-syn from the Tg membranes in lane 3. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

 
{alpha}-Syn Dissociation Is Not Regulated by Ca2+ or by Nucleotide Triphosphates—Because many nerve terminal functions are subject to regulation by Ca2+ influx and the availability of metabolic energy, we examined whether {alpha}-syn translocation is affected by changes in these parameters. No significant differences on {alpha}-syn dissociation were observed in the presence or absence (with EGTA) of 1 mM Ca2+ and in the presence of increasing concentrations of cytosol, suggesting a Ca2+-independent mechanism (Fig. 5A). Next, we assessed the effects of ATP and GTP, their non-hydrolyzable analogs, and their various metabolic products in our assay. We did not observe any significant changes to {alpha}-syn dissociation in the presence of any of these compounds (Fig. 5B), suggesting that {alpha}-syn dissociation occurs independently of high energy phosphates.

Parkinson Disease Mutations Increase Cytosol-induced {alpha}-Syn Translocation—We analyzed whether the PD-associated mutations, A30P and A53T, affect {alpha}-syn dissociation using equivalent amounts of synaptosomal membrane (100 mg of wet synaptosomal membrane weight). Fig. 6 shows that, as with Wt {alpha}-syn, cytosol exposure also increased the dissociation of mutant {alpha}-syn from synaptic membranes. However, the dissociation of both A30P and A53T mutants, which was clearly cytosol-dependent, was significantly greater than that of Wt {alpha}-syn at the higher cytosol concentration (p < 0.01). To exclude the possibility that differences in dissociation rates of {alpha}-syn mutants are due to variations in expression level or subcellular compartmentalization, we determined the distribution of {alpha}-syn in synaptosomes. We first compared {alpha}-syn expression in the cytosolic pool (released by hypotonic lysis) relative to the total synaptosomal {alpha}-syn (solubilized by detergent). Regardless of the transgene, ~30% of {alpha}-syn in isolated nerve terminals is soluble (Fig. 7A); the expression levels of Wt and mutant human {alpha}-syn were indistinguishable. The hypotonically lysed synaptosomes, which represent the {alpha}-syn donor in our dissociation assay, were centrifuged and {alpha}-syn in the pellets measured. Again, there were no significant differences between the membrane fractions of Wt and mutants {alpha}-syn (Fig. 7B). In effect, this result indicates that the clinical mutations do not inherently destabilize the {alpha}-syn interaction with biologically derived particulate fraction, since association was maintained with low cytosol concentrations. Rather, the removal of {alpha}-syn from membranes is a mediated event that is enhanced by the two missense mutations.


   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
REFERENCES
 
Current models of {alpha}-syn lipid interaction predict that the amino-terminal portion of{alpha}-syn assembles into an amphipathic{alpha}-helix that embeds partially into membrane bilayers (8, 10, 11, 16, 34). In accord with this concept, our results show that the majority of synaptosomal {alpha}-syn is associated with membranes in vivo, although substantial portion is freely diffusible within the cytoplasm. How these bound and diffusible pools are maintained and whether regulated exchange occurs between these {alpha}-syn compartments is unclear. Here, we present evidence that {alpha}-syn stably associated with the membrane fraction can be recruited into the soluble fraction in the presence of brain cytosol. This was not due to a detergent action of cytosol because integral membrane proteins, e.g. synaptophysin, remained with the membrane pellet and the process was blocked specifically by antibodies to {alpha}-syn. Because the antiserum alone had no affect on {alpha}-syn distribution in the absence of cytosol, the antibodies likely prevent access of cytosolic factors to membrane {alpha}-syn. Moreover, predigestion of brain cytosol with trypsin or pre-heating at 95 °C eliminated the activity, directly implicating a role for specific cytosolic proteins in controlling {alpha}-syn solubility. The permissive factors appear to be enriched in brain since 6-fold greater level of liver cytosol was required to achieve equivalent {alpha}-syn dissociation as brain cytosol.


Figure 5
View larger version (17K):
[in this window]
[in a new window]
  		FIGURE 5.
Calcium and phosphate high energy did not affect {alpha}-syn dissociation. A, histogram shows mean and S.E. of {alpha}-syn dissociated from synaptic membranes in the presence different concentration of brain cytosol supplemented with 1 mM Ca2+ or 1 mM EGTA. No significant difference was observed between EGTA and Ca2+ treatments (one-way ANOVA, p > 0.5, n = 5). B, the effects of 1 mM adenosine, ATP, ADP, GTP, ATP{gamma}S, and GTP{gamma}S, or 3 mM of AMP-CP (non-hydrolysable ADP analog) and AMP-PNP (the non-hydrolysable ATP analog) on {alpha}-syn dissociation were measured in the presence or absence of cytosol following incubation at 37 °C for 10 min. Neither nucleotide triphosphate nor their non-hydrolyzable analogs or metabolic products affected significantly basal or cytosol-induced {alpha}-syn dissociation (one-way ANOVA, with cytosol p > 0.1, n = 5; without cytosol p > 0.2, n = 5). CTRL, control.

 


Figure 6
View larger version (41K):
[in this window]
[in a new window]
  		FIGURE 6.
Increased dissociation of A30P and A53T {alpha}-syn compared with Wt {alpha}-syn. A, two familial PD-linked mutations increase {alpha}-syn dissociation. Dissociation of {alpha}-syn from equivalent amounts of synaptic membranes (100 mg of wet synaptosomal membrane weight) derived from Wt, A53T, or A30P transgenic {alpha}-syn mice was measured in the presence of various concentrations of cytosol (0.15, 0.5, and 1.5 mg/ml). The top panel shows a representative Western blot of {alpha}-syn in the supernatant (S) and in the membrane pellet (P) after a 10-min incubation at 37 °C (using human specific anti-{alpha}-syn antibody 211). Synaptophysin immunoreactivity reflects synaptic membrane input. The bottom panel illustrates the combined results of four independent experiments. Dissociation of {alpha}-syn with either PD-linked mutation was significantly higher in the presence of 1.5 mg/ml cytosol (***, one-way ANOVA, Bonferroni's test, p = 0.0012) but not in the presence of lower cytosol concentrations (0.15 and 0.5 mg/ml cytosol; one-way ANOVA, p > 0.7).

 
The proteins triggering {alpha}-syn dissociation are in limiting quantity in cytosol and are not regenerated under the conditions of our assay. A single exposure to synaptosome membranes was sufficient to deplete cytosolic capacity to extract membrane {alpha}-syn so that subsequent incubations with fresh membranes yielded no additional soluble {alpha}-syn. In contrast, presynaptic membranes retained ample extractable {alpha}-syn, which could be dissociated with subsequent applications of cytosol. While this suggests that the rate and extent of {alpha}-syn dissociation is primarily dependent on the availability of cytosolic factors, additional studies will be needed to determine whether some of the {alpha}-syn that remains membrane-bound is biochemically distinct from the cytosol-extracted {alpha}-syn.

The cytosolic proteins in our experiments are substantially less concentrated than presynaptic cytosol in vivo (estimated at 200–300 mg/ml)4 as a result of dilution with extraction buffers during isolation and contamination from non-neuronal cells in brain. Arguably, the portion of {alpha}-syn that can potentially be mobilized from membranes is likely to be correspondingly greater in intact cells than the 5% shifts we detected. This is compatible with a previous report by Fortin et al. (35) showing that GFP-tagged {alpha}-syn expressed in primary hippocampal neurons accumulates at synaptic boutons and that the fluorescence at photobleached boutons recovers rapidly as if {alpha}-syn has substantial steady state mobility and can be replenished from adjacent boutons. Neuronal stimulation in the presence of extracellular Ca2+ dispersed the {alpha}-syn fluorescence from presynapses in a tetanus toxin-sensitive manner, suggesting that {alpha}-syn translocation occurred after exocytosis and that Ca2+ entry per se is an insufficient trigger. We also did not observe any Ca2+ dependence or a direct requirement for nucleotide triphosphate cleavage in inducing {alpha}-syn dissociation. Although it is likely that post-exocytotic regulatory aspects are not recapitulated in our assay, our observations demonstrating cytosol-mediated {alpha}-syn dissociation may underlie the movement of GFP-{alpha}-syn during resting conditions or associated with exocytosis (35). Our results suggest a dynamic model of {alpha}-syn interaction with membranes, in which cytosolic proteins control the exchange of membrane-stabilized {alpha}-syn with a diffusible form, and may explain some discrepancies in previous studies regarding the proportions of soluble and bound {alpha}-syn, which ranged from equal distribution (36, 37) to virtually all soluble (13). The fact that most of the {alpha}-syn in synaptosomes is membrane-bound during steady state conditions suggests that net cytosol-induced dissociation is impeded until activation by physiological stimuli and that the equilibrium between membrane and soluble pools is balanced by regulated re-association to membranes.


Figure 7
View larger version (48K):
[in this window]
[in a new window]
  		FIGURE 7.
A, proportion of cytoplasmic and total synuclein is not affected by PD mutations. Top panel, {alpha}-Syn expression levels and distribution in cytoplasm were compared between synaptosomes derived from Wt (lanes 1–3), A30P (lanes 4–6), and A53T (lanes 7–9) {alpha}-syn Tg mice. A representative Western blot showing {alpha}-syn, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and synaptophysin in the supernatant following resuspension of 100 mg (wet tissue weight) intact synaptosomes into isotonic buffer (lanes 1, 4, and 7), hypotonic buffer (lanes 2, 5, and 8), or 1% Triton X-100 (lanes 3, 6, and 9). Bottom panel, quantification of five independent experiments measuring the cytosolic (C; from lanes 2, 5, and 8) and total (T; from lanes 3, 6, and 9) {alpha}-syn. No significant differences were observed between Wt and mutant {alpha}-syn expression (one-way ANOVA, cytosolic fraction, p = 0.51, n = 5; membrane fraction, p = 0.26, n = 5). B, left panel: {alpha}-syn immunoreactivity was compared between pellet fractions of the hypotonically-lysed synaptosomes from A (lanes 2, 5, and 8). Right panel, no significant differences were observed in the amount of membrane bound {alpha}-syn in hypotonically lysed synaptosomes from {alpha}-syn Wt and mutant Tg mice (one-way ANOVA, p > 0.8).

 
The cytosolic activity that mediates {alpha}-syn dissociation clearly distinguishes between the Wt {alpha}-syn and the PD-associated mutants. Both A30P and A53T mutations doubled the cytosol-induced {alpha}-syn off-rate but had no effect on the cytosol-independent dissociation. This was unexpected because several studies, although not all (16), have previously suggested that the A30P mutation (but not A53T) impairs lipid binding (12, 14, 21, 38). Those studies measured the ability of purified recombinant {alpha}-syn to insert spontaneously into purified or artificial lipids and did not account for naturally existing cofactors or chaperones. Indeed, we did not observe differences in Wt or mutant {alpha}-syn expression in our mice or their partitioning between soluble and membrane compartments, in agreement with studies on intact cultured neurons showing no effect on membrane interaction by the A30P mutation (17) and with other transgenic lines where neither the ratio of soluble and particulate {alpha}-syn (39) nor its axonal transport (40) is affected by the A30P mutation. Furthermore, in our experiments, which employ biological membranes and include potential endogenous factors that might participate in {alpha}-syn membrane interactions, A53T {alpha}-syn was as likely to dissociate in the presence of cytosol as A30P {alpha}-syn, suggesting that the mutations may confer a common pathogenic effect that is not evident when purified components are used to assess {alpha}-syn membrane binding.

Our assay relies on {alpha}-syn-deficient cytosol as the acceptor fraction in order to detect the subtle movement that would otherwise be masked by the 30% of presynaptic {alpha}-syn that is freely diffusible in cytoplasm. Although we did not address whether other peripherally associated presynaptic proteins are also recruited into the cytosolic buffer along with {alpha}-syn, this is a likely scenario since several presynaptic proteins are transiently associated with the membrane fraction and each has a distinct mechanism that regulates its direct or indirect membrane interaction (22–24). These are mediated by either a membrane protein complex (e.g. {alpha}/beta-SNAP), phosphorylation (e.g. synapsin), Ca2+ (e.g. CAPS), and nucleotide triphosphate hydrolysis (e.g. NSF, dynamin). In particular, there are similarities between the dissociation of {alpha}-syn and the behavior of rab3, a GTP-binding protein that undergoes regulated dissociation and association from vesicles with the aid of GDP-dissociation inhibitor (25–27). Rab3 lacks a transmembrane domain and its membrane-attachment is afforded by hydrophobic prenyl groups at its carboxyl terminus that insert into lipid bilayers (41). GDP-dissociation inhibitor likely shelters the prenyl moieties from aqueous cytoplasm during extraction of rab3a from membranes. In the case of {alpha}-syn, its amino-terminal is predicted to organize into an amphipathic {alpha}-helix that permits partial insertion of hydrophobic residues into the lipid bilayer (11). Regulated dissociation from membranes would require either unfolding of the {alpha}-helix or segregation of those hydrophobic residues. Both mechanisms are compatible with a requirement for a cognate cytosolic factor that regulates {alpha}-syn solubility. Notably, the differential effect imparted by both PD-linked {alpha}-syn mutations relative to Wt {alpha}-syn in the presence of cytosol implicates the as yet undefined cytosolic activity in the pathogenic process. Our approach described here to assess {alpha}-syn membrane interactions can be used to identify and characterize the cytosolic activity that modulates {alpha}-syn sequestration into and exchange between sub-cellular compartments. Importantly, the underlying mechanism may be amenable to experimental manipulation as a means of influencing {alpha}-syn solubility in vivo so as to prevent its pathogenic accumulation.


   FOOTNOTES
 
* This work was supported by operating grants (to A. T.) from the Canadian Institute of Health Research (CIHR) and the Parkinson Society of Canada. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

1 Postdoctoral support was provided by the Leon Frederick Foundation and the Journal of Cell Science travel grant. Back

2 To whom correspondence should be addressed: Centre for Research in Neurodegenerative Diseases, University of Toronto, 6 Queen's Park Crescent West, Toronto, Ontario M5S 3H2, Canada. Tel.: 416-978-8880; Fax: 416-978-1878; E-mail: a.tandon{at}utoronto.ca.

3 The abbreviations used are: {alpha}-syn, {alpha}-synuclein; PD, Parkinson disease; Wt, wild-type; ANOVA, analysis of variance; Tg, transgenic; ATP{gamma}S, adenosine 5'-O-(thiotriphosphate); GTP{gamma}S, guanosine 5'-3-O-(thio)triphosphate; AMP-PNP, adenosine 5'-(beta,{gamma}-imino)triphosphate; AMP-CP, {alpha},beta-methylene adenosine diphosphate. Back

4 W. Balch, personal communication. Back



   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 REFERENCES
 

  1. Lavedan, C. (1998) Genome Res. 8, 871–880[Abstract/Free Full Text]
  2. Clayton, D. F., and George, J. M. (1999) J. Neurosci. Res. 58, 120–129[CrossRef][Medline] [Order article via Infotrieve]
  3. Bennett, M. C. (2005) Pharmacol. Ther. 105, 311–331[CrossRef][Medline] [Order article via Infotrieve]
  4. Kruger, R., Muller, T., and Riess, O. (2000) J. Neural Transm. 107, 31–40[CrossRef][Medline] [Order article via Infotrieve]
  5. Ueda, K., Saitoh, T., and Mori, H. (1994) Biochem. Biophys. Res. Commun. 205, 1366–1372[CrossRef][Medline] [Order article via Infotrieve]
  6. Dawson, T. M., and Dawson, V. L. (2003) J. Clin. Invest. 111, 145–151[CrossRef][Medline] [Order article via Infotrieve]
  7. Zarranz, J. J., Alegre, J., Gomez-Esteban, J. C., Lezcano, E., Ros, R., Ampuero, I., Vidal, L., Hoenicka, J., Rodriguez, O., Atares, B., Llorens, V., Tortosa, E. G., Del Ser, T., Munoz, D. G., and de Yebenes, J. G. (2004) Ann. Neurol. 55, 164–173[CrossRef][Medline] [Order article via Infotrieve]
  8. Jo, E., McLaurin, J., Yip, C. M., St George-Hyslop, P., and Fraser, P. E. (2000) J. Biol. Chem. 275, 34328–34334[Abstract/Free Full Text]
  9. Eliezer, D., Kutluay, E., Bussell, R., Jr., and Browne, G. (2001) J. Mol. Biol. 307, 1061–1073[CrossRef][Medline] [Order article via Infotrieve]
  10. Chandra, S., Chen, X., Rizo, J., Jahn, R., and Sudhof, T. C. (2003) J. Biol. Chem. 278, 15313–15318[Abstract/Free Full Text]
  11. Jao, C. C., Der-Sarkissian, A., Chen, J., and Langen, R. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 8331–8336[Abstract/Free Full Text]
  12. Kubo, S., Nemani, V. M., Chalkley, R. J., Anthony, M. D., Hattori, N., Mizuno, Y., Edwards, R. H., and Fortin, D. L. (2005) J. Biol. Chem. 280, 31664–31672[Abstract/Free Full Text]
  13. Maroteaux, L., and Scheller, R. H. (1991) Brain Res. Mol. Brain Res. 11, 335–343[Medline] [Order article via Infotrieve]
  14. Jensen, P. H., Nielsen, M. S., Jakes, R., Dotti, C. G., and Goedert, M. (1998) J. Biol. Chem. 273, 26292–26294[Abstract/Free Full Text]
  15. Fortin, D. L., Troyer, M. D., Nakamura, K., Kubo, S., Anthony, M. D., and Edwards, R. H. (2004) J. Neurosci. 24, 6715–6723[Abstract/Free Full Text]
  16. Perrin, R. J., Woods, W. S., Clayton, D. F., and George, J. M. (2000) J. Biol. Chem. 275, 34393–34398[Abstract/Free Full Text]
  17. McLean, P. J., Kawamata, H., Ribich, S., and Hyman, B. T. (2000) J. Biol. Chem. 275, 8812–8816[Abstract/Free Full Text]
  18. Sharon, R., Goldberg, M. S., Bar-Josef, I., Betensky, R. A., Shen, J., and Selkoe, D. J. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 9110–9115[Abstract/Free Full Text]
  19. Lotharius, J., Barg, S., Wiekop, P., Lundberg, C., Raymon, H. K., and Brundin, P. (2002) J. Biol. Chem. 277, 38884–38894[Abstract/Free Full Text]
  20. Iwai, A., Masliah, E., Yoshimoto, M., Ge, N., Flanagan, L., de Silva, H. A., Kittel, A., and Saitoh, T. (1995) Neuron 14, 467–475[CrossRef][Medline] [Order article via Infotrieve]
  21. Jo, E., Fuller, N., Rand, R. P., George-Hyslop, P., and Fraser, P. E. (2002) J. Mol. Biol. 315, 799–807[CrossRef][Medline] [Order article via Infotrieve]
  22. Lin, R. C., and Scheller, R. H. (2000) Annu. Rev. Cell Dev. Biol. 16, 19–49[CrossRef][Medline] [Order article via Infotrieve]
  23. Slepnev, V. I., and De Camilli, P. (2000) Nat. Rev. Neurosci. 1, 161–172[Medline] [Order article via Infotrieve]
  24. Sudhof, T. C. (2004) Annu. Rev. Neurosci. 27, 509–547[CrossRef][Medline] [Order article via Infotrieve]
  25. Araki, S., Kikuchi, A., Hata, Y., Isomura, M., and Takai, Y. (1990) J. Biol. Chem. 265, 13007–13015[Abstract/Free Full Text]
  26. Fischer von Mollard, G., Sudhof, T. C., and Jahn, R. (1991) Nature 349, 79–81[CrossRef][Medline] [Order article via Infotrieve]
  27. Wu, S. K., Zeng, K., Wilson, I. A., and Balch, W. E. (1996) Trends Biochem. Sci. 21, 472–476[CrossRef][Medline] [Order article via Infotrieve]
  28. Kozak, M. (1984) Nucleic Acids Res. 12, 857–872[Abstract/Free Full Text]
  29. Kozak, M. (1991) J. Cell Biol. 115, 887–903[Abstract/Free Full Text]
  30. Scott, M. R., Kohler, R., Foster, D., and Prusiner, S. B. (1992) Protein Sci. 1, 986–997[Medline] [Order article via Infotrieve]
  31. Citron, M., Westaway, D., Xia, W., Carlson, G., Diehl, T., Levesque, G., Johnson-Wood, K., Lee, M., Seubert, P., Davis, A., Kholodenko, D., Motter, R., Sherrington, R., Perry, B., Yao, H., Strome, R., Lieberburg, I., Rommens, J., Kim, S., Schenk, D., Fraser, P., St George, H. P., and Selkoe, D. J. (1997) Nat. Med. 3, 67–72[Medline] [Order article via Infotrieve]
  32. Abeliovich, A., Schmitz, Y., Farinas, I., Choi-Lundberg, D., Ho, W. H., Castillo, P. E., Shinsky, N., Verdugo, J. M., Armanini, M., Ryan, A., Hynes, M., Phillips, H., Sulzer, D., and Rosenthal, A. (2000) Neuron 25, 239–252[CrossRef][Medline] [Order article via Infotrieve]
  33. Tandon, A., Bannykh, S., Kowalchyk, J. A., Banerjee, A., Martin, T. F., and Balch, W. E. (1998) Neuron 21, 147–154[CrossRef][Medline] [Order article via Infotrieve]
  34. Zhu, M., Li, J., and Fink, A. L. (2003) J. Biol. Chem. 278, 40186–40197[Abstract/Free Full Text]
  35. Fortin, D. L., Nemani, V. M., Voglmaier, S. M., Anthony, M. D., Ryan, T. A., and Edwards, R. H. (2005) J. Neurosci. 25, 10913–10921[Abstract/Free Full Text]
  36. Irizarry, M. C., Kim, T. W., McNamara, M., Tanzi, R. E., George, J. M., Clayton, D. F., and Hyman, B. T. (1996) J. Neuropathol. Exp. Neurol. 55, 889–895[Medline] [Order article via Infotrieve]
  37. Kahle, P. J., Neumann, M., Ozmen, L., Muller, V., Odoy, S., Okamoto, N., Jacobsen, H., Iwatsubo, T., Trojanowski, J. Q., Takahashi, H., Wakabayashi, K., Bogdanovic, N., Riederer, P., Kretzschmar, H. A., and Haass, C. (2001) Am. J. Pathol. 159, 2215–2225[Abstract/Free Full Text]
  38. Bussell, R., and Eliezer, D. (2004) Biochemistry 43, 4810–4818[CrossRef][Medline] [Order article via Infotrieve]
  39. Kahle, P. J., Neumann, M., Ozmen, L., Muller, V., Jacobsen, H., Schindzielorz, A., Okochi, M., Leimer, U., van Der, P. H., Probst, A., Kremmer, E., Kretzschmar, H. A., and Haass, C. (2000) J. Neurosci. 20, 6365–6373[Abstract/Free Full Text]
  40. Li, W., Hoffman, P. N., Stirling, W., Price, D. L., and Lee, M. K. (2004) J. Neurochem. 88, 401–410[Medline] [Order article via Infotrieve]
  41. Goody, R. S., Rak, A., and Alexandrov, K. (2005) Cell Mol. Life Sci. 62, 1657–1670[CrossRef][Medline] [Order article via Infotrieve]

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/43/32148    most recent
M605965200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Tandon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Tandon, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement