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Originally published In Press as doi:10.1074/jbc.M509886200 on November 29, 2005

J. Biol. Chem., Vol. 281, Issue 5, 2876-2881, February 3, 2006
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Structure of the N-terminal Calcium Sensor Domain of Centrin Reveals the Biochemical Basis for Domain-specific Function*

Jonathan H. Sheehan{ddagger}1, Christopher G. Bunick{ddagger}1, Haitao Hu{ddagger}12, Patricia A. Fagan§1, Susan M. Meyn{ddagger}, and Walter J. Chazin{ddagger}§3

From the {ddagger}Departments of Biochemistry and Physics and Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232-8725 and the §Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92307

Received for publication, September 8, 2005 , and in revised form, November 28, 2005.


   ABSTRACT
TOP
 ABSTRACT
INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 CONCLUDING REMARKS
 REFERENCES
 
Centrin is an essential component of microtubule-organizing centers in organisms ranging from algae and yeast to humans. It is an EF-hand calcium-binding protein with homology to calmodulin but distinct calcium binding properties. In a previously proposed model, the C-terminal domain of centrin serves as a constitutive anchor to target proteins, and the N-terminal domain serves as the sensor of calcium signals. The three-dimensional structure of the N-terminal domain of Chlamydomonas rheinhardtii centrin has been determined in the presence of calcium by solution NMR spectroscopy. The domain is found to occupy an open conformation typical of EF-hand calcium sensors. Comparison of the N- and C-terminal domains of centrin reveals a structural and biochemical basis for the domain specificity of interactions with its cellular targets and the distinct nature of centrin relative to other EF-hand proteins. An NMR titration of the centrin N-terminal domain with a fragment of the known centrin target Sfi1 reveals binding of the peptide to a discrete site on the protein, which supports the proposal that the N-terminal domain serves as a calcium sensor in centrin.


   INTRODUCTION
TOP
 ABSTRACT
 INTRODUCTION
EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 CONCLUDING REMARKS
 REFERENCES
 
Many dynamic processes in cells are critically dependent on the microtubule-based cytoskeleton. The number, direction, and polarity of the microtubules are regulated by an organelle known as the microtubule-organizing center (MTOC)4. In higher eukaryotes, such as humans, the centrosome functions as the MTOC. Lower organisms possess equivalent MTOCs, notably the basal body in algae and the spindle pole body in yeast. The composition of MTOCs is highly proteinaceous, and there exists significant structural heterogeneity between them in different eukaryotes. Despite this heterogeneity, MTOCs in all eukaryotes contain a number of conserved protein components.

One of these proteins is centrin (also known as caltractin), an EF-hand calcium-binding protein that has been identified in organisms ranging from protozoa and yeast to plants and humans (1, 2). Genetic studies show that centrin is essential to proper cellular division because it regulates the cell cycle-dependent duplication and segregation of the MTOCs (3, 4). Other centrin functions include: (i) initiation of flagellar excision in Chlamydomonas reinhardtii through a fiber-based microtubule severing mechanism (5), (ii) forming part of the human heterotrimeric DNA damage recognition complex required for global genome nucleotide excision repair (6), (iii) modulation of homologous recombination and nucleotide excision repair in Arabidopsis (7), and (iv) involvement with nuclear mRNA export machinery in yeast (8).

Centrin is closely related to the ubiquitous archetypal EF-hand calcium sensor protein calmodulin (CaM). Both proteins are composed of two structurally independent globular domains connected by a flexible linker. Each structural domain contains two helix-loop-helix "EF-hand" calcium-binding motifs (9). Unlike CaM, centrin has a long (~20 residue) positively charged N-terminal extension that is highly disordered in solution. This region has been shown to mediate calcium-dependent polymerization of human centrin2 (hCen2) in vitro (10).

Calcium-induced conformational change is central to the target activation mechanism of CaM and other EF-hand calcium sensor proteins (11). The mechanism for activation of CaM-like calcium sensors involves calcium-induced structural rearrangements within each domain, which lead to the formation of large hydrophobic cavities on the molecular surface that mediate the interaction with target proteins. The interactions of CaM with kinase targets were the first to be characterized structurally and these studies revealed binding through a wrap-around mode where both domains envelop a single helical moiety from the target (12). As additional CaM targets were characterized, other binding mechanisms were observed including extended and dimerization modes (reviewed in Ref. 13). In the case of centrin, there is mounting evidence that the extended binding mode may be more prominent (14-17), and this subject remains an area of intense investigation.

Centrin and CaM have strikingly different calcium binding properties when measured in vitro. CaM has four high affinity calcium-binding sites (designated sites I-IV) with dissociation constants (Kd) in the range of 1-10 µM under physiological salt concentrations (9). The relatively high affinity of CaM for calcium enables it to respond effectively to physiologically relevant increases in the intracellular concentration of free calcium, which typically rises into the range of 1-10 µM. Most centrins, in contrast, have different calcium binding properties, including one or more non-functional calcium binding sites. Two examples emphasize these distinct centrin calcium binding characteristics. 1) The yeast homologue of centrin (cdc31p) has two defective calcium binding sites (sites II and III) based on both mutational studies and primary sequence analysis of the residues comprising the EF-hand loops (15, 18). 2) The C-terminal domain of C. reinhardtii centrin (CRC-C) has substantially lower affinity for calcium in vitro than that observed for typical EF-hand calcium sensors because of the substitution of a highly conserved glutamate with an aspartate at position 12 in site III (16, 19). In canonical EF-hand loops, the glutamate at position 12 provides important bidentate oxygen coordination of calcium. Nevertheless, despite reduced calcium binding affinity in site IV, CRC-C is able to mediate the interaction with Kar1 and the other target proteins reported for centrin (20-24) using the fully functional site IV.

A number of centrin-binding partners have been identified, and the majority of those studied appear to bind primarily via the C-terminal domain. Recently, a peptide corresponding to the cdc31p-binding region of Kar1 (K19) was shown to bind with high affinity only to the C-terminal domain of C. reinhardtii centrin in vitro (17). The calcium affinity in the presence of the target was sufficiently high to warrant the notion that CRC-C is bound to Kar1 at the basal level of calcium in the cell. This observation implies that the N-terminal domain serves as the centrin calcium sensor.

Sfi1 is another known centrin-binding partner, which, like centrin, is an essential component of the yeast MTOC (20). Yeast Sfi1 contains 17 repeats of a centrin binding motif containing the consensus sequence AX7LLX3F/LX2WK/R. It has been shown that the interaction of Sfi1 with centrin is essential for mitosis (20). A model has been proposed for calcium/centrin-dependent contraction of the Sfi1 filament (43), in which it is conceivable that both centrin domains are involved.

We report here the three-dimensional structure of CRC-N in the presence of calcium, determined by solution NMR spectroscopy. The domain occupies an open conformation similar to EF-hand calcium sensor domains. A comparative analysis of the structure reveals why CRC interacts with Kar1 only via its C-terminal domain and identifies structural features that distinguish CRC from CaM and other typical EF-hand calcium sensor proteins. To test the proposal that it serves as a calcium sensor, titrations of CRC-N with the seventh centrin-binding repeat of Sfi1 were performed, using intrinsic tryptophan fluorescence and NMR spectroscopy to characterize the interaction.


   EXPERIMENTAL PROCEDURES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
RESULTS
 DISCUSSION
 CONCLUDING REMARKS
 REFERENCES
 
Recombinant C. reinhardtii centrin N-terminal domain was expressed and purified as described elsewhere (19). The 96-residue construct used in this study consists of residues Met1 through Met94, with an additional Gly-Ser sequence at the N terminus left after cleavage of the His6 tag.

A 24-residue peptide (IVSLKEANLVKRIFHSWKKLLYID) including the seventh centrin-binding repeat of Sfi1 (underlined) was synthesized by Sigma Genosys and further purified by high performance liquid chromatography.

Fluorescence Spectroscopy—All fluorescence experiments were performed on a Spex Fluorolog 1681 fluorimeter (Spex Industries Inc., Edison, NJ) at 20 °C. The excitation wavelength was 285 nm, with slit width set to 2.0 mm. Small aliquots of appropriate dilutions of a 1 mMCRC-N stock solution containing 150 mM KCl and 25 mM Tris at pH 7.1 were added to a 5 µM (initial concentration) Sfi1 peptide solution under identical conditions, then incubated with 1 mM EDTA or 5 mM Ca2+. Corrections for background fluorescence were made by subtracting the spectra from identical solutions without peptide.

NMR Spectroscopy—NMR data were acquired on five different samples15 of CRC-N with the following isotopic compositions: unlabeled; U-15N; U-13C; U-15-N,13C; and 10% 13C. Buffers contained either 10% or 100%2 H2O as appropriate. Each sample typically had a protein concentration of 1-2 mM in a buffer of 25 mM Tris-d11 and 5-10 mM CaCl2 at pH 7.0.

All NMR data were recorded at 25 °C on Bruker DRX600 and DRX800 spectrometers equipped with triple-resonance probe heads and triple axis pulsed field gradient accessories. Backbone sequential assignments of CRC-N were achieved by the combined use of HNCA, CBCANH, CBCA(CO)NH, and HNCO experiments (NMR experiments reviewed in Ref. 25). Aliphatic side chain resonance assignments were obtained from(H)CC(CO)NH, H(CCCO)NH, and HBHA-(CO)NH experiments. 1H chemical shift assignments of aromatic side chains were primarily based on a two-dimensional homonuclear two-quantum experiment. Stereospecific assignments of the valine and leucine methyl groups were achieved using the 10% 13C-enriched sample (26). To obtain NOE-based distance restraints, a two-dimensional homonuclear NOESY experiment was recorded on the unlabeled sample, a three-dimensional 15N NOESY-HSQC on the 15N-enriched sample, and three-dimensional 13C NOESY-HSQC and four-dimensional13C HMQC-NOESY-HMQC on the uniformly 13C-labeled sample. The mixing time used in all NOESY experiments was 100 ms. Standard 15N-1H HSQC spectra were acquired under identical conditions for calcium-loaded U-15N CRC-N the absence and presence of a 3-fold excess of Sfi1 peptide. Data were processed in FELIX (Version 2000; Accelrys, San Diego, CA).

Structure Calculations—NOE assignments were made using FELIX and aided by the program SANE (27). Initial analysis of the NOE data indicated that the first 23 residues (Gly(-2) through Gly21) of the 96-residue CRC-N construct were highly disordered. Consequently, the first 20 residues (Gly (-2) through Gly18) were excluded from the structure calculations. For residues Leu22 through Met94, a total of 1108 NOE-based distance restraints (462 intraresidue, 220 sequential, 208 medium range, and 218 long range) were derived from the suite of NOESY experiments noted above. These restraints were initially assigned conservative upper bounds based on calibration of the NOESY cross-peak intensities against NOE correlations corresponding to known proton-proton distances or distance ranges. They were subsequently fine-tuned through a series of test structure calculations. The final restraint list had upper bounds of 3.5, 4.5, and 6.0 Å for the three-dimensional 15N NOESY-HSQC, three-dimensional 13C NOESY-HSQC, and four-dimensional 13C HMQC-NOESY-HMQC data sets, and 4.5 and 6.0 Å for the two-dimensional NOESY experiment. In addition, 24 distance restraints were generated for 12 hydrogen bonds based on the observation of reduced amide exchange rates and characteristic NOEs (28), and 116 backbone torsion angle restraints, each with an assigned minimum range of ±30°, were obtained from 1H{alpha}, 13C{alpha}, 13Cbeta, 13C', and 15N chemical shifts using the program TALOS (29).

Initial structures of CRC-N were generated using DYANA (30) and subsequently refined with the program AMBER (31) (Version 7.0) using a 20-ps simulated annealing protocol described previously (16). Out of the 50 structures generated, 45 converged properly to a single fold. The program FINDFAM (32) was then used to establish that at least 20 structures were needed to adequately represent the ensemble. The final representative ensemble of 20 structures was selected on the basis of minimal constraint violation energies. All of these structures also had favorable (low) molecular energies in the AMBER force field.

Structure Analysis—Graphical analysis of the structures was carried out using the program MOLMOL (33). The stereochemistry of the final family of structures was assessed using the software PROCHECK NMR (34). Inter-helical angles were calculated using the program INTERHLX (Kyoko Yap, University of Toronto). ClustalW (35) was used for multiple-sequence alignments. Homology models were constructed using the SWISS-MODEL module at ExPASy (36). Structures used in the comparative analyses2+ were obtained from the Protein Data Bank: apo CaM(1CFC [PDB] ), Ca2+-CaM (1CLL), Ca2+-CaM/R20 complex (1CDL), Ca2+-TnC (1TN4), and Ca2+-S100B (1MHO).

Data Deposition—The 1H, 13C, and 15N chemical shift assignments have been deposited with the BioMagResBank under accession number 6918. The coordinates of the final family of 20 structures and the full list of NMR restraints used in the structure calculations have been deposited with the Protein Data Bank under accession code 2AMI.


   RESULTS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
DISCUSSION
 CONCLUDING REMARKS
 REFERENCES
 
The high resolution three-dimensional structure of calcium-loaded CRC-N was determined by multidimensional heteronuclear NMR spectroscopy. The favorable line widths and excellent dispersion exhibited in the NMR spectra greatly facilitated the chemical shift assignments and NOE analysis. Nearly complete 1H, 13C, and 15N resonance assignments were obtained for CRC-N. A total of 1248 (>16/residue) experimentally derived NMR restraints (1108 NOE-based distance restraints, 24 hydrogen bond distance restraints, 116 backbone torsion angle restraints) were used in the structure calculations. The final representative ensemble of structures exhibits low constraint violation energies, with no distance violations >0.2 Å and no torsion angle violations >5°, and low molecular energies (-1072 ± 26 kcal-mol-1)in the AMBER force field. Analysis by PROCHECK NMR shows that 91.5% of all backbone torsion angles, excluding proline and glycine residues, fall within the most favored regions of the Ramachandran plot. The structural statistics for the final ensemble are summarized in Table 1.


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  		TABLE 1
Statistics for the (Ca2+)2-CRC-N structural ensemble

 
A stereo view of the family of 20 conformers that represent the structure of (Ca2+)2-CRC-N is shown in Fig. 1. The secondary structure elements of CRC-N are defined to a high level of precision, whereas the two calcium-binding loops, the linker connecting the two EF-hands, and the two termini are less well defined (Table 1). Not surprisingly, the overall fold of CRC-N is very similar to other EF-hand proteins, with the two EF-hands packed against each other to form a single structural domain. This compact packing of the pair of EF-hands into a four-helix bundle is important for function (37).

One important means to classify EF-hand calcium binding proteins is via their inter-helical angles. To this end, inter-helical angles were calculated for calcium-loaded CRC-N and compared with CRC-C and the representative calcium sensors: CaM, TnC, and S100B (Table 2). The comparison of inter-helical angles shows that CRC-N occupies an open conformation more similar to CRC-C and the individual domains of calcium-bound CaM than those of apo-CaM and the alternate "S100-type" calcium sensor. This analysis confirms that CRC-N adopts an open conformation in the calcium-loaded state, consistent with calcium sensor activity.


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  		TABLE 2
Comparison of inter-helical angles in CRC-N, CaM, TnC, and S100Ba

 


Figure 1
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  		FIGURE 1.
Stereo view of the final ensemble of 20 conformers representing the solution structure of CRC-N (residues Leu22-Met94).

 
To further test the ability of CRC-N to serve as a calcium sensor, experiments were performed to characterize CRC-N binding of a peptide fragment of Sfi1 corresponding to the seventh centrin binding repeat. This repeat was chosen because it is closest to the consensus AX7LLX3F/LX2WK/R sequence. Addition of calcium-free CRC-N to a solution of the Sfi1 peptide had no effect on the intrinsic tryptophan fluorescence. However, repeating this experiment using calcium-loaded CRC-N resulted in a substantial quenching of fluorescence and a shift in {lambda}max from 355 to 335 nm (Fig. 2A). Fitting of the titration data to a standard binding curve indicates a dissociation constant in the micromolar range. Note that it is difficult to judge the significance of this value because centrin is anticipated to be pre-associated with Sfi1 via the C-terminal domain at the basal level of Ca2+.

To obtain further insight into the nature of the binding of the Sfi1 peptide to CRC-N, an NMR titration of the peptide into a solution of calcium-loaded, 15N-enriched CRC-N was performed. Consistent with the fluorescence experiments, perturbations in the NMR signals of CRC-N were observed only in the presence of calcium. Fig. 2B shows an overlay of the 15N-1H HSQC spectrum of calcium-loaded CRC-N in the absence and presence of the Sfi1 peptide. The observation of perturbations of only a subset of CRC-N signals indicates that the peptide binds to a discrete binding site on the protein.

Table 3 lists all residues whose chemical shifts can be positively identified as perturbed by the addition of the Sfi1 peptide. Remarkably, residues are perturbed in each of the structural elements of the protein, even the unstructured N-terminal region (Met1-Gly21). To analyze these data, it is important to recognize that the origin of perturbations in NMR chemical shifts is a combination of changes in the environment due to the presence of a ligand and any alterations in the conformation of the protein. In many cases, NMR chemical shift perturbations provide direct insights into the location of the ligand binding site because the structure of the protein is not significantly affected. However, for Sfi1 binding to CRC-N, the widespread distribution of chemical shift perturbations suggests that the conformation of protein changes in concert with binding of the peptide and limits the ability to define the binding site in the absence of additional experimental data such as intermolecular NOEs.


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  		TABLE 3
Chemical shift perturbations of CRC-N upon peptide binding

 

   DISCUSSION
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
CONCLUDING REMARKS
 REFERENCES
 
Previous studies of centrin have shown that its N- and C-terminal domains have a number of distinct features (10, 17, 19, 38-40, 44). For example, a number of centrin targets have been shown to interact through the C-terminal domain. Among these studies, the interaction with the core centrin-binding region of Kar1 has been most extensively characterized (1). A 19-residue peptide fragment, K19, binds to the C-terminal domain of centrin with high affinity but shows no appreciable affinity for CRC-N (17).


Figure 2
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  		FIGURE 2.
Binding of Sfi1 peptide to CRC-N. A, intrinsic fluorescence of the peptide in the absence (black) and presence (red) of calcium-loaded CRC-N. B, overlay of a region of the 600 MHz HSQC NMR spectrum of 15N-labeled CRC-N in the absence (black) and presence (red) of the Sfi1 peptide.

 
In contrast to Kar1 and other C-terminal domain-specific targets, some centrin targets may bind via contacts with both domains. Sfi1 is one such protein. Like centrin, Sfi1 is an essential component of the yeast MTOC (20). Yeast Sfi1 contains 17 copies of a consensus repeat sequence that binds centrin. It has been shown that the interaction of Sfi1 with centrin is essential for mitosis, and a model has been proposed involving calcium-dependent contraction of the Sfi1 filament (43). We have previously obtained evidence suggesting that CRC-C can bind targets at the basal level of calcium in the cell (17). In this study we show that the consensus repeat 7 of Sfi1 binds to a specific site on CRC-N only in the presence of calcium. Thus, our results support the proposal by Salisbury (43) and suggest a specific model in which the centrin C-terminal domain serves as an anchor and the N-terminal domain serves as the calcium sensor.

Insights into the biochemical basis for differences between the two centrin domains are evident from sequence analysis combined with comparative analysis of their 3D structures. An alignment of CRC-N and CRC-C sequences is shown in Fig. 3A. A notable property of CRC-C is that it possesses only two of the four well conserved methionine residues found in CRC-N and other CaM-like calcium sensor domains, which are purported to play a key role in target binding (41, 42). Position 127, which is an alanine in CRC-C but a Met in CRC-N, is an important residue in CRC-C. This small residue is associated with the unique secondary hydrophobic pocket in the CRC-C binding site, into which the Leu13 side chain of K19 is deeply inserted. Fig. 3b shows that in CRC-N this pocket is filled in (by Met54). This difference in the CRC-N and CRC-C structures is fully consistent with the lack of high affinity binding to Kar1 and the concept of domain-specific binding targets for centrin.

The differences between CRC-N and CRC-C extend beyond the unique secondary hydrophobic binding pocket. To fully understand the properties that distinguish CRC-N, additional comparative structural analyses were carried out with other calcium sensor domains. Returning to inter-helical angles, although CRC-N clearly occupies an open conformation, careful inspection reveals significant differences relative to CRC-C and the domains of CaM and TnC (Table 2). Of note in these comparisons are the consistent differences in the critical I/II and III/IV inter-helical interfaces, which are larger by 28° and 17° in the case of CRC-N versus CRC-C and by 27° and 24° in the case of CRC-N versus CaM-N.

Sequence alignment of the domains of CRC and CaM provides further insight into the significant difference in the interface between helices I and II. One readily apparent difference is a well conserved valine in CRC-C and the CaM domains, which is substituted by a smaller alanine in CRC-N (Ala53) (Fig. 3A). Fig. 4 shows a comparison of the side chain packing in the inter-helical interface between helices I and II in CRC-N and CaM-N. The smaller Ala53 side chain in CRC-N allows the two helices to pack more closely against each other, which correlates with the observed larger inter-helical angle (Table 2). This contributes significantly to CRC-N appearing to be "less open" than CRC-C, CaM-N, and other calcium sensor domains.


Figure 3
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  		FIGURE 3.
CRC-N has unique structural features. A, sequence alignment of CRC-N with CRC-C, CaM-N, and CaM-C. Note within the structural boundaries of helix II that there are two highlighted (red and blue) sequence positions. In the red position of helix II there is an alanine in CRC-N (Ala53), while the other three domain sequences contain a valine. In the blue position of helix II there is an alanine in CRC-C (Ala127), while the other three domain sequences contain a methionine (Met54 in CRC-N). B, molecular surface representations of CRC-N and CRC-C with the hydrophobic accessible surface depicted in yellow. Circles are drawn at the location of the Met for Ala substitution: CRC-N (left, Met54) and CRC-C (right, Ala127).

 


Figure 4
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  		FIGURE 4.
Differences in the inter-helical packing between helices I and II in CaM-N (left) and CRC-N (right). CRC-N has an alanine at position 53 in place of a well conserved valine in CaM-N, CaM-C, and CRC-C. This sequence difference allows closer packing of helix II against Phe37 in helix I in CRC-N. The arrow indicates the shift in packing of this conserved Phe ring.

 


Figure 5
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  		FIGURE 5.
Comparison of the N- and C-terminal domains of CaM, CRC, and hCen2. The electrostatic potential surfaces are displayed for CRC-N (2AMI), CRC-C (1OQP), CaM-N (1CLL), CaM-C (1CLL), hCen2-N (modeled on our CRC-N structure), and hCen2-C (2A4J). The gradient in the electrostatic charge potential is depicted as color gradients, red for negative charge and blue for positive charge.

 
Molecular surface representations in Fig. 5 show that CRC-N has a unique, relatively flat binding surface, unlike the concave binding surfaces observed in other calmodulin family calcium sensor domains. In the case of CRC-N, the binding pocket is filled in by several long side chains, such as Met54,Met69, Ile70, Ile73, and Lys93. An analysis of multiple sequence alignments of 30 different centrins (structbio.vanderbilt.edu/chazin/cabp_database/seq/align/centrin.aln.html) reveals a high degree of conservation of these side chains. Indeed, a homology model of hCen2-N shows surface and charge characteristics very similar to CRC-N in Fig. 5 because many critical residues in the binding site are conserved. These observations suggest that the unique features observed for CRC-N are likely conserved among all centrins.

The electrostatic potential surface of CRC-N is compared with those of CRC-C, CaM-N, and CaM-C in Fig. 5. CRC-N is the least negatively charged among the four domains. This fact is most evident on the face of the putative CRC-N target-binding site, which contains a large basic patch. The concentration of basic residues in the CRC-N target binding surface contrasts starkly with the largely acidic nature of the binding site in CRC-C and the two CaM domains. The opposite charge properties of the two CRC domains are consistent with our proposal that the CRC-N has the potential to recognize targets that are distinct from CRC-C and the CaM domains.

To obtain further insight into probable determinants of binding specificity, Fig. 6 shows the sequences of the two peptides (from Kar1 and Sfi1) that have been characterized in detail by biophysical methods. Three hydrophobic residues in K19 provide key stabilizing contacts in the high resolution structure of the, complex with CRC-C, including Trp10 Leu13, and Leu14. In the alignment of the two peptides, the three residues in Sfi1 are found to be identical, and the Sfi1 peptide does indeed bind to CRC-C5.

Why then does the Sfi1 peptide interact with CRC-N but the Kar1 peptide does not? The critical factors are evidently due to differences elsewhere in the sequence. For example, residues immediately N-terminal to the Trp anchor are quite different between Kar1 and Sfi1, with the three-residue gap of particular importance (Fig. 6). The evidence from our NMR titration of the Sfi1 peptide with CRC-N reveals there are many residues perturbed that lie outside the archetypal target binding surface, including a number of residues in the unstructured N-terminal residues (1-21). The latter is intriguing because the sole functional property associated with this region to date is calcium-dependent oligomerization of human centrin 2 observed only in vitro in the absence of any other proteins (10). Since NMR chemical shift perturbations can arise from either direct contacts with ligand or allosteric structural perturbations, the structural implications cannot yet be specified from available data. Regardless of the structural details, the data do strongly imply that Sfi1 interacts with CRC-N in a manner different from the complex of K19 with CRC-C.


Figure 6
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  		FIGURE 6.
Sequence comparison of Kar1 and Sfi1 peptides. The pairwise alignment of K19 and Sfi1r7c highlights identical (red) and conserved (magenta) residues in each peptide.

 

   CONCLUDING REMARKS
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 CONCLUDING REMARKS
REFERENCES
 
The structure reported here along with the demonstration of calcium-dependent binding of the Sfi1 peptide and several other lines of evidence provide strong support for the proposal that the N-terminal domain of centrin serves as a calcium sensor. This hypothesis is consistent with the domain-specific functional model of centrin. The centrin C-terminal domain serves as an anchor that is constitutively bound even at the basal level of calcium in the cell (17). Our new data provide evidence supporting the role of the N-terminal domain as the calcium-sensitive regulatory element. Experiments to test and refine this hypothesis are urgently required and will enable the in-depth knowledge of centrin structure and biochemistry to be used to better understand its cellular functions.


   FOOTNOTES
 
The atomic coordinates and structure factors (code 2AMI) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

* This work was supported by Operating Grants RO1 GM-40120 and GM-65484 (to W. J. C.) and Vanderbilt Center in Molecular Toxicology Grant P30 ES0000267 (for facilities) from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Back

1 These authors contributed equally to this research. Back

2 Present address: Discovery Chemistry Research and Technologies, Lilly Research Laboratories, Indianapolis, IN 46285. Back

3 To whom correspondence should be addressed. Tel.: 615-936-2210; Fax: 615-936-2211; walter.chazin{at}vanderbilt.edu.

4 The abbreviations used are: MTOC, microtubule-organizing center; CRC-C, 77-residueC-terminal fragment of Chlamydomonas reinhardtii centrin; CRC-N, 94-residue N-terminal fragment of Chlamydomonas reinhardtii centrin; hCen2, human centrin iso-form-2; CaM, calmodulin; TnC, troponin C; cdc31p, the yeast homologue of centrin; K19, 19-residue cdc31p-binding domain of Kar1; HSQC, heteronuclear single quantum coherence; NOE, nuclear Overhauser effect. Back

5 S. Meyn and W. J. Chazin, unpublished data. Back


   ACKNOWLEDGMENTS
 
We acknowledge the support and contributions of Vincent Lee and Bessie Huang at the early stages of this research and the kind hospitality of David A. Case.



   REFERENCES
TOP
 ABSTRACT
 INTRODUCTION
 EXPERIMENTAL PROCEDURES
 RESULTS
 DISCUSSION
 CONCLUDING REMARKS
 REFERENCES
 

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