| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 282, Issue 22, 16454-16464, June 1, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11
123
From the
Center for Molecular Neurobiology, the Department of Molecular and Cellular Biochemistry, and the Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio 43210
Received for publication, January 8, 2007 , and in revised form, April 3, 2007.
 |
ABSTRACT |
|---|
 TOP ABSTRACT INTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
30% of total tau converted into detergent-insoluble aggregates. Agonist addition also led to rapid losses in the tubulin binding activity of tau, although tau was not hyperphosphorylated as judged by occupancy of phosphorylation sites Ser396/Ser404. Tau aggregation was associated with decreased viability as detected by ToPro-3 uptake. The results, which establish a new approach for analysis of tau aggregation in cells independent of tau hyperphosphorylation, suggest that conformational changes associated with aggregation are incompatible with microtubule binding, and that toxicity associated with intracellular tau aggregation is not acute but develops over a period of days. |
INTRODUCTION |
|---|
|
TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
-sheet conformation. As in other filamentous aggregates, the resultant -sheets stack in parallel, with each sheet orthogonal to the axis of the growing tau filament (2). Neurofibrillary lesion formation is accompanied by 4–8-fold increases in total brain tau levels (3, 4). Because expression of total tau does not change at the mRNA level in AD (5, 6), disease-associated increases in tau levels may derive from post-translational events (e.g. fibrillization) that selectively decrease tau turnover (7).
Tau fibrillization correlates spatially and temporally with neurodegeneration and cognitive decline (8, 9). As a result, tau lesion densities are used for postmortem staging and diagnosis of AD (10). These correlations, along with the discovery that mutations in the tau gene cause familial forms of frontotemporal dementia (11–13), suggest that tau misfunction is somehow associated with neurodegeneration. Three general hypotheses have been put forward to rationalize a direct relationship between tau fibrillization and disease. The first argues that tau aggregates and other misconformers are directly toxic to cells that harbor them. For example, tau aggregates are capable of inhibiting the ubiquitin-proteasome system (7). Inhibition of this system, which plays important roles in cell homeostasis, has been proposed to be a source of cellular stress (14, 15). The second argues that sequestration of tau from microtubules destabilizes the cytoskeleton leading to a loss-of-function toxicity (16). Although loss of tau is well tolerated over both long (17, 18) and short (19) time periods, simultaneous loss of both tau and other microtubule-associated proteins may lead to more severe phenotypes (20). A third hypothesis is that tau modified post-translationally (e.g. by hyperphosphorylation or truncation) may be directly toxic to cells (21, 22). Under these conditions, aggregation could be protective for cells by sequestering toxic species.
Resolving the role of tau fibrillization in disease is important for assessing the potential of tau-directed therapies. However, directly testing the above hypotheses in biological models has been complicated by the resistance of full-length tau isoforms to spontaneous aggregation over experimentally tractable time periods (23). The problem has been overcome by high level tau overexpression alone (24, 25) or in combination with aggregation promoting mutations (26). Conditions that drive aggregation-promoting post-translational modifications (e.g. phosphorylation (27)) also have been successful. But the efficiency of fibrillization is frequently low, whereas mere overexpression of normal tau in neurons results in a toxic phenotype (28). Because levels of normal tau protein do not increase in AD (4), the significance of overexpression-mediated toxicity found in transgenic systems has been ambiguous.
Recently we found that the kinetic barrier to full-length tau fibrillization can be overcome by small-molecule ligands that bind -sheet structures such as CR (Congo red), thiazin red, and thioflavin S (23). The ability of CR to populate amyloidgenic protein conformations (29) suggests that these agents drive aggregation by thermodynamic linkage of their binding reaction with the tau self-association reaction (linkage refers to the influence of ligand binding on other equilibria including aggregation reactions and phase transitions (30)). As a result, fibrillization agonists facilitate the study of full-length tau fibrillization in the absence of post-translational modifications, mutations, or macromolecular inducers such as heparin (23).
Here we extend this approach to cell culture as a means of examining the role of full-length tau fibrillization on acute toxicity. The results suggest that large scale aggregation in HEK-293 cells leads to significant losses in cell viability over a period of days.
|
EXPERIMENTAL PROCEDURES |
|---|
|
TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
-tubulin antibody MAB1864 (33) was from Chemicon International (Temecula, CA), whereas monoclonal mouse anti--tubulin antibody DM-1A (34) along with protease inhibitors (1x complete contained: 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride, 0.8 µM aprotinin, 40 µM bestatin, 20 µM leupeptin, 15 µM pepstatin A, 14 µM L-transepoxysuccinyl-leucylamido-[4-guanidino]butane, and 26 µM N-acetyl-leu-leunorleu-al) and poly-D-lysine were from Sigma. Alexa 546-conjugated goat anti-mouse IgG secondary antibody and fluorescent probe ToPro-3 were from Invitrogen, whereas Cy5-conjugated goat anti-rat IgG secondary antibody and Vecta Shield were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) and Vector Laboratories (Burlingame, CA), respectively. Purified bovine brain tubulin was from Cytoskeleton (Denver, CO). CR (City Chemical LLC, West Haven, CT) was stored as a 50 mM stock solution in Me2SO. Recombinant htau40 containing polyhistidine-tagged htau40 (His6-htau40) was prepared as described previously (35).
In Vitro Tau Assembly—His6-htau40 (1 µM) was incubated with CR for varying time periods in assembly buffer (10 mM HEPES, pH 7.4, 100 mM NaCl, 5 mM dithiothreitol) at 37 °C. Aliquots were removed and assayed for solubility by centrifugation (described below) or for fibrillization by electron microscopy as described previously (23, 36). Images were viewed in a Phillips CM 12 transmission electron microscope operated at 65 kV, and captured on film at 22,000-fold magnification. Five fields were imaged per sample. All filaments in each image were counted and average total length adsorbed per field (
f) was calculated ± S.D.
Cell Culture—HEK-293 cells were obtained from ATCC (Manassas, VA), whereas an HEK-293-derived cell line stably expressing human htau40 (stable tau cells) was prepared as described previously (37). A stable tetracycline-inducible htau40 cell line (inducible tau cells) was prepared in Trex-293 cells (Invitrogen) following the manufacturer's instructions and the same plasmid construct used to create stable tau cells. All cell lines were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin G, 250 ng/ml amphotericin B, and 0.1 mg/ml streptomycin (37 °C with 5% CO2). Stable tau cells were grown under selection with 0.5 mg/ml G418, whereas inducible tau cells were grown in 5 µg/ml blasticidin and 0.4 µg/ml zeocin. Tau expression was induced in these cells with tetracycline (1 µg/ml). To assay the effect of CR on tau aggregation and cell viability, all cells were plated and cultured for 24 h, treated with varying concentrations of CR, and harvested after 1–14 days of treatment. CR was refreshed with each change of media.
Isolation of Soluble and Insoluble Tau—The solubility of cell-derived tau protein was assayed under microtubule-destabilizing conditions. CR-treated tau cells were suspended in lysis buffer (20 mM Tris-HCl, pH 7.4, 140 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3NO4, 10 mM NaF, 0.5% v/v Nonidet P-40, 1 mM EDTA, and 1x complete protease inhibitors), subjected to three freeze/thaw cycles (38), and then incubated at 4 °C for 30 min to induce the disassembly of microtubules. Nuclei were removed from the lysates by centrifugation at 1000 x g for 5 min at 4 °C.
The resultant extracts were centrifuged (100,000 x g for 1 h) at 4 °C. The resultant pellets were washed three times with lysis buffer, then resuspended in lysis buffer (1/3, v/v, relative to the total volume of supernatant). Equal volumes of pellet and supernatant fractions were boiled and subjected to immunoblot analysis as described below. Aliquots of in vitro aggregation reactions were processed similarly after centrifugation, except that pellets were washed with PBS before being resuspended in SDS-sample buffer.
Immunoelectron Microscopy—Tau aggregates were isolated from CR-treated tau cells by a modification of the procedure used to isolate authentic PHF (39). Cells were homogenized in ice-cold buffer H (10 mM Tris-HCl, 1 mM EGTA, 0.8 M NaCl, 10% sucrose and protease inhibitor mixture, pH 7.4), and centrifuged for 20 min at 20,000 x g. Supernatant fractions (S1) were saved, whereas pellet fractions (P1) were re-homogenized in buffer H and separated into S2 and P2 fractions by centrifugation (20 min at 20,000 x g). Supernatant fractions S1 and S2 were combined and incubated with 1% Sarkosyl for 1 h at room temperature with agitation. After centrifugation of the mixture (1 h at 100,000 x g), the resultant pellets were resuspended in 50 mM Tris-HCl (pH 7.4). Aliquots were absorbed onto Formvar/carbon-coated grids and incubated with the Tau12 monoclonal antibody for 30 min at 37 °C. After washing with 0.1% gelatin, grids were incubated with the 10-nm gold-labeled goat anti-mouse IgG + IgM (Amersham Biosciences). Samples were then stained with 2% uranyl acetate for 1 min, and viewed by electron microscopy as described above.
Tubulin Assays—To assess tubulin binding activity of tau aggregates prepared in vitro, His6-htau40 (1 µM) treated with CR (10 µM) for various lengths of time was incubated with 1 mg/ml purified tubulin at 37 °C for 30 min in binding buffer (80 mM PIPES, pH 6.8, 1 mM MgCl2, 1 mM EGTA, 1 mM GTP) in a final volume of 100 µl. Reactions were incubated with 1 µg of monoclonal anti--tubulin antibody for 2 h after which protein G-agarose beads (40 µl of 25% (w/v) slurry) were added and incubation continued for 1 h. Immunoprecipitates were collected by centrifugation (2000 x g for 5 min), washed three times with lysis buffer, and finally subjected to immunoblot analysis as described below. For these experiments, input tubulin was visualized by Coomassie Blue staining after SDS-PAGE.
To assay the influence of aggregation on the ability of tau to bind endogenous tubulin, lysates (100 µg) were prepared from CR-treated or non-treated stable tau cells as described above, after which tubulin was immunoprecipitated with anti--tubulin antibody and protein G-agarose as described above. For these experiments, levels of endogenous tubulin cell lysates were estimated by immunoblot analysis with anti--tubulin antibody.
To assay tubulin polymerization, 1 mg/ml tubulin was incubated (37 °C) in binding buffer containing 10% glycerol with or without 10 µM CR. The time course of polymerization was followed as classically described (40), except that absorbance was measured at 380 nm (instead of 350 nm) to minimize the absorbance contribution from CR.
Immunoblot Analysis—Samples were boiled in SDS-PAGE loading buffer, subjected to SDS-PAGE, and transferred to polyvinylidene difluoride membranes as described previously (37). Protein bands were visualized by enhanced chemiluminescence and quantified with a Bio-Rad GS-800 calibrated laser densitometer.
Cell Death Assay—Inducible tau cells grown in the presence or absence of 10 µM CR and tetracycline inducer for up to 4 days were isolated by trypsinization, harvested by centrifugation, resuspended in PBS, dispensed in triplicate into 96-well black-walled flat bottom plates (Costar) at initial seeding densities of 
100,000 cells/well, and incubated with ToPro-3 (5 µM for 30 min), a fluorescent DNA-intercalating dye taken up by dead but not live cells (41, 42). ToPro-3 uptake was then measured directly in a FlexStation plate reader (
em = 640 nm; ex = 670 nm) relative to a cell-free blank reaction.
Immunocytochemistry—Cells were plated on poly-D-lysine-coated glass coverslips for 24 h, then grown in the presence or absence of 10 µM CR for up to 4 days. Cells were then harvested at various time points, chilled on ice for 30 min, washed three times with PBS, and finally fixed with methanol at -20 °C. Following PBS washes, cells were sequentially labeled with primary monoclonal antibodies Tau5 (1:200) and MAB1864 (1:200) and secondary antibodies Alexa 546-labeled goat anti-mouse IgG (1:400) and/or Cy5-conjugated goat anti-rat IgG (1:400) in PBS containing 3% bovine serum albumin. When present, ToPro-3 was incubated at 5 µM for 1.5 h. Coverslips were then mounted with Vectashield and visualized with a Leica TCS SL laser-scanning confocal system operated at wavelengths optimized for simultaneous detection of Alexa 546 (ex = 546 nm; em = 555–620) and Cy5 (ex = 633 nm; em = 650–750) or ToPro-3 (ex = 640 nm; em = 650–750 nm) in the presence of tau-bound CR (ex = 488 nm; em = 500–535).
Analytical Methods—Aggregation progress curves were modeled as a monomolecular growth scheme assuming pseudo-first order kinetics. Filament growth was fit to the function,
 |
correspond to filament length or mass at time t, at time 0, and at infinite time, respectively, and kapp is the apparent first-order rate constant. For in vitro reactions, y0 was always set equal to zero.
Consistent with this model, time-dependent loss of soluble tau was fit to a simple exponential decay function,
 |
All linear and non-linear regression fits are reported ± S.E. of the estimate. The probability that an observed difference in the mean of replicates was statistically significant was determined by Student's t test.
|
RESULTS |
|---|
|
TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
6-fold more potent than thiazin red as a tau fibrillization inducer (23), and established an optimum CR concentration range of 10–20 µM. The time course of CR-mediated aggregation (1 µM His6-htau40) was estimated at 10 µM CR. At these reactant concentrations, fibrillization proceeded without a perceptible lag and yielded well resolved filaments for the first 48 h. After that time, however, filaments formed tangled masses that were impossible to quantify by transmission electron microscopy methods (Fig. 1C). When the first 48 h of reaction was modeled as a first-order approach to plateau using the monomolecular equation (Equation 1), kapp, the first-order rate constant for growth of the filament population was 0.28 ± 0.03 h-1. Together these data demonstrate that fibrillization of micromolar concentrations of full-length tau protein can be rapidly induced by 10 µM CR under near physiological conditions.
|
Congo Red Induces Tau Aggregation in Cell Culture—To determine whether CR could induce tau aggregation in cell culture, HEK-293 cells stably overexpressing the longest human tau isoform (stable tau cells) were incubated with varying concentrations of CR for 7 days. HEK-293 cells were chosen for this experiment because they tolerate high-level tau overexpression (5.3 ± 0.6 pg/cell, n = 4 determinations, data not shown) without serious toxicity (Ref. 37 and see below). Cell lysates were then prepared in nonionic detergent Nonidet P-40, separated into soluble and insoluble fractions by ultracentrifugation (1 h at 100,000 x g), and subjected to immunoblot analysis using anti-tau and anti--tubulin monoclonal antibodies. Insolubility in both ionic and nonionic detergents is a characteristic of authentic AD-derived tau filaments (43, 44). Results showed that Tau5 immunoreactivity appearing in the pellet fraction had a biphasic dependence on CR concentration (Fig. 3A). Insoluble tau was not a result of cosedimentation with microtubules, because lysates were prepared under microtubule depolymerizing conditions where most tubulin remained in the soluble fraction (Fig. 3B). The optimum CR concentration for inducing insoluble cellular tau was similar to the optimum determined in vitro (Fig. 3C).
|
|
|
|
|
To characterize the cellular distribution of tau aggregates, wild-type HEK-293 cells or stable tau cells grown in the presence or absence of 10 µM CR for up to 7 days were subjected to confocal immunocytochemistry using monoclonal antibodies against tau (Tau5) and tubulin (MAB1864). MAB1864 was used in place of DM-1A because its rat isotype facilitated double labeling in the presence of mouse monoclonal IgGs such as Tau5. Immunoreactivity was detected with secondary antibodies linked to fluors Alexa 564 and Cy5 so as to minimize overlap with CR autofluorescence. In the absence of tau expression (HEK-293 cells), tubulin staining predominated at the cell periphery (Fig. 5A). CR treatment of these cells did not alter their microtubule staining pattern, and free intracellular CR did not autofluoresce at visible excitation/emission wavelengths (shown for ex = 488; em = 500–535 in Fig. 5B). In cells expressing htau40 (tau stable cells) in the absence of CR, Tau5 immunostaining colocalized with tubulin (Fig. 5C). After a period of days in the presence of 10 µM CR, however, tau-positive inclusions appeared in the cytoplasm where they colocalized with CR autofluorescence (maximal at ex = 488 under these conditions) but not tubulin (Fig. 5D).
|
Tau Aggregation Reduces Its Tubulin-binding Activity—To determine the effects of CR-induced tau fibrillization on tau function, the ability of His6-htau40 treated with CR in vitro for up to 24 h to bind purified tubulin was examined by immunoprecipitation using -tubulin antibody DM-1A. In the absence of CR, tau co-immunoprecipitated with tubulin (Fig. 7A). In contrast, the presence of CR led to time-dependent losses in levels of tubulin-bound tau (Fig. 7A), with the initial rate of loss of tubulin-binding activity being faster that the rate of loss of tau from the soluble fraction (Fig. 7B). Loss of tau-binding activity was not the result of direct CR-mediated inhibition of tubulin polymerization, because CR at 10 µM was incapable of antagonizing spontaneous tubulin polymerization in the absence of tau (Fig. 7C). These data indicated that it was the products of CR/tau interaction that were incompatible with tubulin binding.
To confirm these findings, lysates prepared from tau cells grown in the presence or absence of CR for 7 days were subjected to tubulin immunoprecipitation with anti--tubulin antibody DM-1A followed by immunoblot analysis with antibody Tau5. Over this time period, the presence of CR yielded a doubling of total tau levels relative to cells incubated in its absence (Figs. 4 and 8). Nonetheless, the amount of endogenous tau that immunoprecipitated with endogenous tubulin decreased over 30% (Fig. 8). Together these data suggest that the change in tau conformation or aggregation state induced by CR diminished tubulin binding affinity relative to tau monomer in random coil conformation.
Tau Aggregation Decreases Cell Viability—To determine the effects of tau aggregation on cell viability, tetracycline-inducible tau cells were treated with or without 10 µM CR for up to 4 days and then incubated in the presence of ToPro-3, a nuclear stain (38). Inducible cells were used so that the tau dependence of CR-mediated toxicity could be more tightly controlled. Assay was limited to a 4-day time period so that dead cells would not be underestimated because of media change.
|
ex = 640 nm; em = 670 nm) did not overlap with the autofluorescence of CR observed in the presence of tau. Nonetheless, ToPro-3 is a cyanine dye, and certain cyanines are capable of interacting with tau protein (53). To exclude cross-reactivity between ToPro-3 and tau aggregates as a source of nonspecific signal intensity, inducible cells treated with tetracycline to express tau protein were incubated with or without CR for 4 days, then fixed, incubated with ToPro-3, and finally examined by confocal microscopy. In the absence of CR, ToPro-3 selectively labeled cell nuclei and did not colocalize with cytoplasmic tau immunofluorescence (Fig. 9A). In the presence of CR, CR-positive tau inclusions formed, but ToPro-3 staining remained exclusively nuclear (Fig. 9B). These data suggest that the ToPro-3 fluorescence remains dependent on access to nuclei and is not influenced by the presence of CR or tau aggregates. To assess the effect of CR-mediated tau aggregation on cell viability, inducible cells were treated with and without tetracycline inducer of tau expression in the presence and absence of CR. ToPro-3 fluorescence intensity was linearly related to the number of cells assayed under all conditions (Fig. 10A). However, the relationship between cell number and fluorescence intensity (i.e. the slope) depended on the cell population investigated. Compared with non-tau expressing cells grown in the absence of CR, the presence of either CR or the induction of tau expression in the absence of CR led to a modest 1.6 ± 0.1-fold increase in ToPro-3 fluorescence intensity (p < 0.01; Fig. 10, A and B), suggesting weak toxicity associated with these experimental conditions relative to untreated and uninduced controls. In contrast, tau overexpression in the presence of CR led to a 5.9 ± 0.2-fold increase in ToPro-3 fluorescence intensity compared with non-tau expressing cells grown in the absence of CR (p < 0.001; Fig. 10, A and B). These levels were significantly greater than those for either non-induced cells grown in the presence of CR or induced cells grown in the absence of CR (p < 0.001), and also greater than the effects of CR treatment and tau overexpression combined. Together these data suggest that changes in tau conformation and aggregation state produce a tau-dependent decrease in cell viability.
|
DISCUSSION |
|---|
|
TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
115 h in HEK-293 cells, which would be 1–2 orders of magnitude longer than the half-life for nonaggregated tau (62, 63).
|
5.6 pg/cell. Assuming a typical HEK-293 cell volume of 4 pl (66, 67), these levels correspond to intracellular tau concentrations (25 µM) well above estimates of physiological tau concentrations in cells (68) and also adult brain homogenates (3). In either case, however, the amount of free tau is unknown. The ability of agonist inducers to support tau aggregation as low as 200–300 nM htau40 (23) suggests that it may be possible to induce tau aggregation at more physiological bulk tau concentrations in the future. Third, CR treatment does not lead to tau hyperphosphorylation as judged by occupancy of sties Ser396/Ser404 and so may not capture potential toxicity of phosphoaggregates. For example, high level accumulation of phospho-tau aggregates have been suggested to sequester PIN1 as part of their mechanism of toxicity (69). Still, there are many sites beyond Ser396/Ser404 that are at least partially occupied in cells and these may change in the presence of CR. Finally, CR appears to drive homogeneous nucleation of assembly competent species, whereas the association of PHFs with cytomembranes (70) in AD tissue is more consistent with a heterogeneous nucleation mechanism (71). Experience with -amyloid peptide suggests that it is the location of active fibrillization, rather than accumulation of filaments per se, that influences toxicity (72). If so, then site of nucleation may have crucial effects on cell phenotype. Heterogeneous interactions may contribute to toxicity in part by controlling the location of nucleation and extension reactions, and homogeneous nucleation paradigms may miss this aspect of toxicity. With these limitations in mind, the results found here in HEK-293 cells are significant in two respects. First, they indicate that changes in full-length tau conformation and aggregation can decrease cell viability. The toxicity is not acute, but develops over a period of days and is statistically significant by 4 days in culture. The results are consistent with the long-term toxicity associated with the tau conformational change and aggregation in some transgenic mouse models (28). Most importantly, toxicity did not depend on direct modulation of signal transduction pathways, suggesting that tau aggregation/conformational change can influence cell viability. It will be important to extend these findings to neurons. In AD, >90% of cortical tau aggregation localizes to neuropil threads and dystrophic neurites associated with neuritic plaques, indicating that most tau aggregation occurs in specialized processes rather than in cell bodies (73). These aggregates may affect end points other than viability that are nonetheless important for disease progression, including retraction and loss of dendritic arbors (74) and synaptic contacts (75). For example, intracellular aggregates formed from polyglutamine repeats can inhibit processes especially important for neuronal homeostasis such as axoplasmic flow (76). The small-molecule agonist method of inducing fibrillization described here should be portable to neuronal cultures for examining these aspects of toxicity.
Second, the results indicate that the initial rate of loss of tubulin binding affinity exceeds the rate of disappearance of soluble tau in the presence of CR. This suggests that small detergent-soluble tau aggregates formed early in the reaction pathway, or perhaps the conformation of tau monomer stabilized by the presence of CR has a lower affinity for tubulin binding than random coil tau monomer irrespective of phosphorylation state. Although it has been proposed that tau fibrillization begins in association with microtubules (77), the observed incompatibility between tau aggregation and microtubule binding suggests that microtubule dissociation precedes aggregation. This would not be surprising, because the same segment of tau that mediates tubulin binding also mediates polymerization (1).
|
|
FOOTNOTES |
|---|
1 These authors contributed equally to this work.
2 Current address: Dept. of Neuropathology, The Johns Hopkins University, Baltimore, MD.
3 To whom correspondence should be addressed: 1060 Carmack Rd., Columbus, OH 43210. Tel.: 614-688-5899; Fax: 614-292-5379; E-mail: kuret.3{at}osu.edu.
4 The abbreviations used are: AD, Alzheimer disease; CR, Congo red; HEK, human embryonic kidney; PHF, paired helical filament; ToPro-3, quinolinium, 4-[3-(3-methyl-2(3H)-benzothiazolylidene)-1-propenyl]-1-[3-(trimethylammonio)propyl]-diiodide; PBS, phosphate-buffered saline; PIPES, 1,4-piperazinediethanesulfonic acid.
|
ACKNOWLEDGMENTS |
|---|
|
REFERENCES |
|---|
|
TOPABSTRACTINTRODUCTIONEXPERIMENTAL PROCEDURESRESULTSDISCUSSIONREFERENCES |
|---|
) and pellet (•) fractions. Each data point represents the mean ± S.D. of quadruplicate determinations, whereas the lines represent best fit to monomolecular growth (solid line; 
). Each point represents the mean ± S.D. of four independent experiments. CR treatment lead to time-dependent increases in total cellular tau levels, largely because of formation of insoluble tau. After 7 days incubation, 
, tau- and CR-;