|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
J. Biol. Chem., Vol. 282, Issue 37, 26822-26831, September 14, 2007
Negative Transcriptional Regulation of Multidrug Resistance Gene Expression by an Hsp70 Protein*From the Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242
Received for publication, June 11, 2007 , and in revised form, July 16, 2007.
One of the most common origins of multidrug resistance occurs via the overproduction of ATP-binding cassette (ABC) transporter proteins. These ABC transporters then act as broad specificity drug pumps and efflux a wide range of toxic agents out of the cell. The yeast Saccharomyces cerevisiae exhibits multiple or pleiotropic drug resistance (Pdr) often through the over-production of a plasma membrane-localized ABC transporter protein called Pdr5p. Expression of the PDR5 gene is controlled by two zinc cluster-containing transcription factors called Pdr1p and Pdr3p. Cells that lack their mitochondrial genome ( 0 cells) strongly induce PDR5 transcription in a Pdr3p-dependent fashion. To identify proteins associated with Pdr3p that might act to regulate this factor, a tandem affinity purification (TAP) moiety was fused to Pdr3p, and this recombinant protein was purified from yeast cells. The cytosolic Hsp70 chaperone Ssa1p co-purified with TAP-Pdr3p. Overexpression of Ssa1p repressed expression of PDR5 but had no effect on expression of other genes involved in the Pdr phenotype. This Ssa1p-mediated repression required the presence of Pdr3p and did not influence Pdr1p-dependent gene expression. Loss of the nucleotide exchange factor Fes1p mimicked Ssa1p-mediated repression of PDR5. Co-immunoprecipitation experiments indicated that Ssa1p was associated with Pdr3p but not Pdr1p in yeast cells. Finally, 0 cells had less Ssa1p bound to Pdr3p than + cells, consistent with Ssa1p-mediated repression of Pdr3p activity serving as a key regulatory step in control of multidrug resistance in yeast.
The same similarities to mammalian cells that have made yeast a powerful model eukaryotic organism restrict the ability to design antifungal drugs without also impacting the health of the animal host. This has led to a limited repertoire of antifungal drugs and makes the development of drug-resistant fungi a special burden on chemotherapy of infections by these organisms. Even more serious is the acquisition of fungi with a multidrug resistance phenotype that can permit tolerance to many antifungal agents with a single genetic change (reviewed in Ref. 1). Saccharomyces cerevisiae mutant strains with a single nuclear mutation have been isolated which enable these mutants to become tolerant of a wide range of toxic compounds. These mutant cells are referred to as having a pleiotropic drug resistant (Pdr)2 phenotype (reviewed in Ref. 2). Mutations either within genes encoding transcriptional regulators of PDR genes or in their regulatory inputs led to overexpression of downstream transporter proteins with associated multidrug resistance. The most extensively studied gene contributing to this pathway in S. cerevisiae is PDR5, which encodes an ATP-binding cassette (ABC) transporter that exhibits broad spectrum drug efflux activity (3–5). Transcription of PDR5 and other Pdr pathway genes are controlled in large measure by the Zn(II)2Cys6 zinc finger regulators Pdr1p and Pdr3p (reviewed in Refs. 6 and 7).
Substitution mutant forms of Pdr1p and Pdr3p transcription factors have been identified that lead to high level overexpression of Pdr5p with an associated increase in multidrug resistance (8–10). Genetic experiments indicate that these mutant transcription factors behave as dominant, hyperactive forms of the regulatory proteins. Pdr1p and Pdr3p share partially over-lapping function and control expression of their target genes by binding to a sequence element referred to as the Pdr1p/Pdr3p response element (PDRE) (11, 12). The PDR3 gene itself is also controlled by two PDREs in its promoter and involves an autoregulatory loop (13). The relative ease of isolation of these hyperactive transcription factors led to the suggestion that both Pdr1p and Pdr3p are subject to negative regulation under normal laboratory conditions, with this negative input eliminated in these mutants (9, 14).
In an effort to find negative regulators of Pdr5p, it was observed that mutants lacking their mitochondrial genome (
The mitochondrial regulation of Pdr3p function represents the first physiological context in which the Pdr pathway is activated. Analyses of the expression of Pdr3p demonstrate that levels of this protein are maintained at an extremely low level in
Yeast Strains and Media—Yeast strains used in this study are listed in Table 1. Cells were grown in cultures containing YPD (2% yeast extract, 1% peptone, and 2% glucose) under nonselective conditions or appropriate SC media under selective conditions (20). Drug resistance was measured by the spot test assay on plates with either a single concentration of drug or gradient plates (21). Transformation was performed using the LiOAc technique (22). Assays for -galactosidase activity were carried out on permeabilized cells using o-nitrophenyl-D-galactopyranoside as substrate as described (23).
Plasmids—The myc-tagged PDR1 clone was designated pPS1 and was constructed in pRS315 (24). The NsiI and SmaI 4.5-kb fragment of plasmid pFL36-1 (provided by Dr. Karl Kuchler) was inserted into a pRS315 subclone of PDR1 plasmid cut with same enzymes. The hyperactive allele of PDR3 was tagged with HA by subcloning a 1-kb SalI-SphI fragment from pXTZ134 (19) into a pRS315-PDR3–11 plasmid and named pPS4. The YEp351-SSA1 plasmid was from Elizabeth Craig. Ace1-2x HA-PDR3 (18), PDR5-(11), SNQ2-(25), and YOR1-lacZ (26) plasmids are from previous studies.
TAP-tagged PDR3 Strain Construction—PDR3 was TAP-tagged as described by Puig et al. (27). Briefly, plasmid pBS1761 was used to amplify the tag by N-TAP Pdr3 for and rev primer GACAACTGCATCAGCAGTTTTATTAATTTTTTCTTATTGCGTGACCGCAgaacaaaagctggagctcat and TTGACACATGCTGTCGAAACTTTTGATCTAGTTGATTTCTTCACTTTCATcttatcgtcatcatcaagtg, respectively. The amplified
Purification of TAP-tagged Pdr3p—Purification of TAP-tagged Pdr3p was modified from Rigaut et al. (28). Protein extractions for the strain carrying the TAP-tagged PDR3 allele were performed as described (27). Cells were grown in rich medium containing galactose as carbon source, so as to induce the GAL-TAP-PDR3 fusion gene. After protein extraction, 500 µl of immunoglobulin G (IgG)-Sepharose (Sigma) was added to 500 mg of total protein in a volume of 25 ml. This slurry was incubated at 4 °C, rotating overnight. After incubation, the resin was washed three times with 50 bead volumes of IPP150 (10 mM Tris·HCl, pH 8.0, 150 mM NaCl, 0.1% Nonidet P-40), and once with 50 bead volumes of tobacco etch virus protease cleavage buffer (10 mM Tris·HCl, pH 8.0, 150 mM NaCl, 0.1% Nonidet P-40, 0.5 mM EDTA, 1 mM dithiothreitol). The IgG-Sepharose was resuspended in 1 ml of tobacco etch virus protease cleavage buffer containing 100 units of tobacco etch virus protease (Invitrogen) and incubated at room temperature, rotating, for 1.5 h. The supernatant was recovered, and added to 3 ml of calmodulin binding buffer (10 mM Proteins were separated by 10% SDS-PAGE and visualized by silver staining (29). Proteins were identified at the University of Iowa Molecular Analysis Facility. Polypeptides of interest were excised and in-gel digested with trypsin following the procedure of Shevchenko et al. (30). The digested proteins were identified using MALDI time-of-flight mass spectrometry as described by Kinter et al. (31). Samples were analyzed on a Bruker Daltonics, Inc. Biflex III mass spectrometer. Real-time PCR—Total RNA was isolated from each fraction with an RNeasy kit (Qiagen). Synthesis of cDNA was performed using iScript cDNA synthesis kit (Bio-Rad). 1 µg of RNA was used as template in each sample with 1x iScript reaction mix and 1 µl of Moloney murine leukemia virus-derived reverse transcriptase. The reaction mix was incubated for 5 min at 25 °C, 30 min at 42 °C, and 5 min at 85 °C. For the quantitative PCR reaction, primers were designed using the Primer Select program from DNASTAR. Primer concentrations were optimized for each gene and annealing profiles were analyzed to evaluate nonspecific amplification by primer dimers. Control reactions, including RNA instead of cDNA were performed for each gene and condition. The threshold cycle (Ct) values were determined in the logarithmic phase of amplification for all genes, and the average Ct value for each sample was calculated from three replicates. The Ct value of the gene coding for actin (ACT1) was used for normalization of variable cDNA levels, and induction factors were determined for each gene and condition by three independent experiments. Primers used were ACT1For (5'-TTGGCCGGTAGAGATTTGACTGAC-3') and ACT1Rev (5'-AGCGGTTTGCATTTCTTGTTCG-3'). The samples were prepared by adding to the cDNA 12.5 µl of the reaction mixture, containing 1x iTaq SYBR Green Supermix with ROX (Bio-Rad) and 200 nM of the both forward and reverse oligonucleotides. PDR5 forward (5'-GAATCATTTGGCGGAAGTAGCA-3') and PDR5 reverse (5'-CCAAAGCGGTAGCGGAATC-3'); or SNQ2 forward (5'-TGCCTGGCTTCTGGACATTC-3') and SNQ2 reverse (5'-TGAGCCGTTTGGTGGGTTGA-3'). H2O was added to reach the final volume of 15 µl. Each standard sample was prepared in triplicate using serial dilutions starting from 100 ng and ending at 10 ng. The unknown samples were also prepared in triplicate with 50 ng of cDNA and the same reaction mixture as the standard samples. Amplification was carried out in the iCycler apparatus from Bio-Rad, in a two-step process as follows: a denaturation step of 3 min at 95 °C, and 40 cycles of 95 °C for 10 s, with annealing and extension at 60 °C for 45 s each. The reporter signals were analyzed using the iCycler iQ software (Bio-Rad). These values can be translated into a quantitative result by constructing a standard curve, with the standard sample values. A melting curve was obtained after completion of the cycles to verify the presence of a single amplicon. The presented RT-PCR results are mean values of at least three independent experiments.
Co-immunoprecipitation Assay—All immunoprecipitation assays were performed using lysed spheroplasts. In brief, cells growing in log phase were washed with spheroplast solution I (1 M sorbitol, 10 mM MgCl2,30 mM dithiothreitol, 100 µg/ml phenylmethylsulfonyl fluoride, 50 mM K2HPO4), resuspended in spheroplast solution II (1 M sorbitol, 10 mM MgCl2,30 mM dithiothreitol, 100 µg/ml phenylmethylsulfonyl fluoride, 50 mM K2HPO4, 25 mM sodium succinate, pH 5.5) containing oxylyticase and incubated at 30 °C for 30 min. After chilling on ice for 10 min, cell suspensions were overlaid on a sucrose cushion (20 mM HEPES, 1.2 M sucrose, 0.02% sodium azide). Spheroplasts were pelleted by centrifuging at 5000 rpm for 20 min at 4 °C in a Beckman JA-20 rotor. These spheroplasts were either stored at –80 °C or lysed immediately using Nonidet P-40 lysis buffer (1% Nonidet P-40/Triton X-100, 0.15 M NaCl, 50 mM Tris-HCl, pH 7.2). Glass beads were added to cells suspended in Nonidet P-40 lysis buffer, followed by addition of 2 mM EDTA, 200 µM sodium vanadate, and 50 mM sodium fluoride. Lysis was performed by shaking cell suspensions on a Tomy shaker at 4 °C. Protein extracts were clarified by centrifuging lysates at 14,000 rpm for 5 min in an Eppendorf microcentrifuge. For immunoprecipitation, washed protein A or G beads were treated with either anti-HA, rabbit anti-Ssa1p (from Elizabeth Craig), or anti-Myc antibody for 2 h. These beads were then mixed with cell lysates and incubated for 4 h. Finally, the beads were washed and immunoprecipitated proteins were recovered by adding 3x Laemmli dye (0.125 M Tris, pH 6.8, 4% SDS, 20% glycerol, 10%
The Hsp70 Protein Ssa1p Co-purifies with Pdr3p—Genetic experiments strongly suggest that Pdr3p is negatively regulated to maintain this factor in a state of low transcriptional activity. First, missense mutations in various regions of Pdr3p or loss of the mitochondrial genome convert this protein into a hyperactive and constitutive positive regulator of expression (8, 10, 15). Second, rapid depletion of the mitochondrial inner membrane chaperone protein Oxa1p accomplished by use of a temperature-sensitive OXA1 allele (19) triggers rapid induction of PDR5, in a manner believed to be dependent on Pdr3p. To determine if other proteins might associate with Pdr3p to repress the activity of this transcriptional regulator, we constructed a TAP-tagged (27) allele of PDR3. The TAP moiety allows gentle purification measures to be used to help maintain protein-protein interactions and has been successfully utilized to purify rare proteins (28). Expression levels of Pdr3p are very low (32) and to facilitate isolation of the TAP-Pdr3p protein, initial experiments used the GAL promoter to drive this fusion gene. The fusion protein was functional and activated PDR5 expression on galactose-containing medium (data not shown). Cells containing the TAP-tagged PDR3 were grown, induced with galactose, and lysed. Protein extract was purified using the two-step IgG-Sepharose and calmodulin resin affinity steps as described previously (27) from a 10-liter culture. Polypeptides co-purifying with the TAP-Pdr3p chimera were recovered and subjected to MALDI mass spectrometry to determine their identity. This mass spectrometric analysis indicated that the Hsp70 protein Ssa1p was found in fractions enriched in TAP-Pdr3p and suggested that these proteins might be associated. An important qualification of this analysis comes from the fact that Ssa1p and Ssa2p are 99% identical (33), so it is probable that both or either of these proteins may associate with Pdr3p. SSA1 and SSA2 differ primarily in their regulation and deletion of both these genes is required to uncover phenotypes dependent on these Hsp70 proteins (34, 35). Our analysis will focus on Ssa1p, but it is likely that Ssa2p can also bind and regulate Pdr3p. This point will be considered in the discussion below. To confirm that this association of Ssa1p with Pdr3p has functional relevance, we tested the effect of elevating Ssa1p levels on Pdr3p-dependent drug resistance. Increase in Cycloheximide Sensitivity of Cells Overproducing Ssa1p—To test the contribution of Ssa1p to control of Pdr3p-dependent PDR5 regulation, we overproduced Ssa1p using a high copy number plasmid containing SSA1 in an isogenic series of strains with varying levels of Pdr3p-dependent transactivation. Transformants were grown to mid-log phase, and equal number of cells were spotted on YPD plates containing 0.4 µg/ml 4-nitroquinoline-N-oxide, 75 µM CdSO4, 0.25 µg/ml cycloheximide or a gradient of this drug and incubated for 2 days at 30 °C (Fig. 1).
Cells lacking their mitochondrial genome (
Overexpression of Ssa1p Leads to Reduced PDR5 Expression in a Pdr3p-dependent Manner—To examine the influence of Ssa1p on Pdr3p transcriptional activation, expression of several different genes known to be Pdr3p-responsive was assessed. Previous work has established that the ABC transporter-encoding genes YOR1 and SNQ2, along with PDR5, are regulated by Pdr3p transcriptional control (reviewed in Refs. 7, 38). We used gene fusions between the promoters of these genes and lacZ to evaluate their response to elevated Ssa1p levels. Wild-type cells were transformed with these reporter plasmids and a high copy number plasmid vector containing or lacking the SSA1 gene. Transformants were grown to mid-log phase, and
Overproduction of Ssa1p lowered PDR5-dependent
PDR5 expression is responsive to transcriptional activation mediated by both Pdr1p and Pdr3p (11, 36, 39). To assess the relative contributions of these transcription factors to the observed effect of Ssa1p overproduction on PDR5 expression, a series of isogenic
In + cells grown on glucose-containing medium, Pdr1p and Pdr3p contribute roughly equally to expression of PDR5 (11, 36). Both wild-type and pdr1 cells were found to exhibit 50% less PDR5-dependent -galactosidase activity when Ssa1p was overproduced (Fig. 2). Importantly, neither a pdr3 nor a pdr1 pdr3 double mutant strain was found to be significantly influenced by elevating the level of Ssa1p. This same qualitative response was observed when the isogenic 0 cells were assayed for PDR5 expression. The magnitude of PDR5-lacZ enzyme activities was much higher, because Pdr3p is activated in the 0 genetic background. Only 0 cells, in which Pdr3p was still expressed, showed a decrease in -galactosidase enzyme activity. We interpret these data to argue that elevation of Ssa1p levels led to a decrease in Pdr3p-dependent transactivation of the PDR5 promoter, whether Pdr3p is in the low activity state ( + cells) or the high activity state ( 0 cells). Pdr1p function was not detectably influenced by changes in Ssa1p levels.
The effect of Ssa1p overproduction on expression of the native PDR5 mRNA was also evaluated to eliminate any possible complications that could arise from use of the lacZ gene fusions. Real-time quantitative RT-PCR was employed to measure mRNA levels for the PDR5 and SNQ2 genes. Levels of the actin transcript (ACT1) were also determined to provide an internal control that does not change in response to Pdr pathway activity. Isogenic
Overproduction of Ssa1p lowered the relative PDR5 transcript levels to
PDR5 Expression Is Reduced in fes1
Fes1p was demonstrated to serve as a nucleotide exchange factor for Ssa1p (45). Additionally, work from several laboratories identified the Sse1p/Sse2p proteins as a second Ssa1p nucleotide exchange factors (46–48). Our attempts to use sse1 mutants to assess the effect of loss of this important Ssa1p regulator were compromised by the poor growth characteristics of these sse1 mutants (data not shown). For this reason, we focused our attention on Fes1p. Fes1p binding to Ssa1p triggers ADP release and is believed to inhibit Ssa1p ATPase activity. Interestingly, fes1 strains have been reported to exhibit cycloheximide sensitivity and temperature-sensitive growth (45). We constructed a fes1 mutation in our standard wild-type genetic background to determine the consequences of loss of normal Hsp70 activity regulation to the observed influence of Ssa1p on PDR5 expression. Isogenic wild-type and fes1 strains were tested for their relative drug resistance in response to normal SSA1 gene dosage but with or without the Fes1p nucleotide exchange factor, respectively. Cells were grown to mid-log phase and assayed for drug resistance as described above (Fig. 4).
Loss of FES1 led to hypersensitivity to cycloheximide as described before (45). To determine if the fes1
The presence of the fes1 Ssa1p Interacts Preferentially with Pdr3p in Vivo—The biochemical data described above, suggesting that Ssa1p directly binds to Pdr3p, were derived from cells overproducing this transcriptional regulator. Additionally, the genetic epistasis experiments indicate that, although Pdr3p is required for the influence of Ssa1p on PDR5 gene expression, its close relative, Pdr1p, is not. Pdr1p and Pdr3p share 36% sequence identity across their lengths (36). To evaluate the interaction of Pdr1p and Pdr3p with Ssa1p under normal expression levels of all proteins, co-immunoprecipitation analysis was performed. Epitope-tagged versions of Pdr1p and Pdr3p were used that have been previously demonstrated to faithfully reproduce the regulator behaviors of the native proteins (19, 32). Both epitope-tagged alleles were carried on low copy number plasmids and were under control of their wild-type promoter sequences.
The plasmids described above were introduced into a pdr1
HA-tagged Pdr3p was recovered in the anti-Ssa1p immuno-precipitate indicating that these two proteins associate in vivo. As predicted by the previous genetic data, no association of Pdr1p and Ssa1p could be detected. These data confirm that the association of Pdr3p and Ssa1p occurs under conditions of normal expression of each protein. Even though Pdr1p and Pdr3p share 36% sequence identity and Pdr1p is estimated to be expressed at a level 10-fold that of Pdr3p under these conditions (15), no Ssa1p-Pdr1p interaction was seen. This reflects the specificity of the Pdr3p-Ssa1p complex seen in these cells.
The Binding of Ssa1p to Pdr3p Is Reduced in
To examine the regulated association of Ssa1p and Pdr3p, we wanted to avoid the complication that is produced by the positive autoregulation of PDR3. Because Pdr3p levels are induced by >10-fold in 0 cells, this would create a problem in terms of assuring equal levels of Pdr3p in immunoprecipitates to be assayed for levels of associated Ssa1p. The autoregulatory circuit was eliminated by replacing the PDREs in the PDR3 promoter with binding sites for the copper-inducible Ace1p transcription factor (50). These binding sites will be referred to as metal-response elements (MREs). This construct has been previously characterized and confers copper-regulated Pdr3p production on cells (18). This plasmid will be referred to as MRE-HA-Pdr3p and was constructed in the low copy number vector pRS315 backbone, which served as a control for this analysis.
Isogenic pdr1
Although equal levels of HA-Pdr3p were recovered by anti-HA immunoprecipitation, less Ssa1p was found in the immunoprecipitates from
The role of Hsp70 proteins in maturation of steroid hormone receptors is a well known example of the function of these chaperones in transcription factor regulation (reviewed in Ref. 51). More recently, it has been appreciated that Hsp70 proteins can influence the activity of many transcription regulatory proteins, including heat shock transcription factor (reviewed in Ref. 52), GATA-1 (53), and S. cerevisiae Hap1p (54). The experiments reported here add the zinc cluster-containing Pdr3p transcription factor to the list of Hsp70 client proteins and implicate Ssa1p/2p as functional regulators of multidrug resistance in S. cerevisiae.
The role of Ssa1p in regulation of Hap1p has been extensively analyzed (54–57). Ssa1p is found associated with Hap1p in a constitutive fashion that does not respond to changes in Hap1p transcriptional activation (58). This is in marked contrast to the interaction of Pdr3p with Ssa1p. Pdr3p:Ssa1p association decreased in The finding that association with Ssa1p acts to inhibit Pdr3p activity has important implications for the understanding of the molecular basis of the many hyperactive dominant forms of Pdr3p that have been described (8–10). Single amino acid substitution mutations have been found that convert Pdr3p to a strong activator of downstream gene expression and drug resistance. These mutations are scattered across the C-terminal region of Pdr3p and have eluded a simple explanation to explain their increased transcriptional activation. We hypothesize that these gain-of-function mutant forms of Pdr3p may be due to decreased Ssa1p binding as a result of changes in the conformation of the central regulatory domain of this transcription factor. We are currently testing this hypothesis. Pdr1p shares 36% sequence identity with Pdr3p across the length of the factors and is capable of heterodimerization (32). Analogous hyperactive mutant forms of Pdr1p have also been isolated that convert this protein to a strong activator of target gene transcription (9). Interestingly, not only do we find that Ssa1p fails to associate with Pdr1p, previous work has demonstrated that Pdr1p is positively regulated by another Hsp70 protein called Ssz1p (17). Although both Pdr1p and Pdr3p are regulated by Hsp70 proteins, these chaperone proteins have opposite effects on the transcriptional regulators of multidrug resistance. Ssz1p seems most likely to exert its positive effect on Pdr1p via an indirect mechanism, because this Hsp70 protein is a component of a set of interacting proteins called the ribosome-associated complex (60), which is required for normal protein synthesis. Additionally, localization data indicated that the majority of Ssz1p was found in the cytoplasm (61), consistent with the important role of this protein in translation. However, some experiments have argued that only extra-ribosomal Ssz1p is responsible for control of drug resistance (62) and suggest the possibility that Ssz1p might more directly interact with Pdr1p, which is found in the nucleus (61, 63). Further investigation is required to evaluate the basis of the positive regulation of Pdr1p by Ssz1p. Association of overproduced proteins with Hsp70 family members can occur due to the role of these chaperone proteins in translation or protein folding. We believe that Ssa1p-Pdr3p association reflects an authentic regulatory interaction for these two proteins for several reasons. First, binding can be demonstrated when Pdr3p and Ssa1p are expressed at normal chromosomal levels. Second, high level expression of Ssa1p exerts a negative effect on Pdr3p- but not Pdr1p-responsive gene expression. Third, loss of the Fes1p nucleotide exchange factor, which blocks the normal catalytic cycle of cytosolic Hsp70 members (like Ssa1p), leads to a decrease in drug resistance and a drop in PDR5 expression. Finally, overproduction of Ssa1p lowered cycloheximide resistance but had no significant effect on other resistance phenotypes such as 4-nitroquinoline-N-oxide or cadmium (data not shown). Cells overproducing Ssa1p exhibited no change in growth properties in the absence of drug challenge, consistent with high levels of this chaperone being well tolerated by the cell. The finding that Ssa1p negatively regulates Pdr3p is unexpected when considering the levels of expression of these two proteins. Estimates using TAP-tagged proteins indicate that Ssa1p levels are roughly 1000 times that of Pdr3p (64). This does not account for the levels of Ssa2p that are similar to those of Ssa1p. Because we believe that either Ssa1p or Ssa2p can negatively regulate Pdr3p, the combined levels of these two chaperone proteins are several orders of magnitude greater than the level of Pdr3p. This vast difference in abundance reflects the wide range of activities carried out by the Ssa1p/2p Hsp70 proteins and is illustrative of the fact that control of Pdr3p activity is but one of these many functions.
The integration of Ssa1p/2p chaperones with Pdr3p is also likely to have important consequences to our understanding of the mitochondrial control of multidrug resistance called retrograde regulation (reviewed in Ref. 65). In S. cerevisiae and the pathogenic yeast Candida glabrata (66), loss of mitochondrial DNA (
* This work was supported by National Institutes of Health Grant GM49825. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 To whom correspondence should be addressed: Dept. of Molecular Physiology and Biophysics, 6-530 Bowen Science Bldg., 51 Newton Road, Iowa City, IA 52242. Tel.: 319-335-7874; Fax: 319-335-7330; E-mail: scott-moyerowley{at}uiowa.edu.
2 The abbreviations used are: Pdr, pleiotropic drug resistance; ABC, ATP-binding cassette; PDRE, Pdr1p/Pdr3p response element; TAP, tandem affinity purification; HA, hemagglutinin; RT, reverse transcription; MRE, metal-response element; MALDI, matrix-assisted laser desorption ionization.
We thank Elizabeth Craig, Karl Kuchler, and Jeff Brodsky for providing reagents and strains; Rob Piper, Jeff Brodsky, and William Walter for helpful discussions and advice; and Yalan Li and the University of Iowa Molecular Analysis Facility for carrying out the mass spectrometric analysis.
This article has been cited by other articles:
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||