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J Biol Chem, Vol. 274, Issue 31, 22089-22094, July 30, 1999

Overactivation of Phospholipase C-1 Renders Platelet-derived Growth Factor -Receptor-expressing Cells Independent of the Phosphatidylinositol 3-Kinase Pathway for Chemotaxis^*

Lars Rönnstrand, Agneta Siegbahn^§, Charlotte Rorsman, Matilda Johnell^§, Klaus Hansen, and Carl-Henrik Heldin

From the Ludwig Institute for Cancer Research, Biomedical Centre, Box 595, S-751 24 Uppsala, Sweden and the ^§ Department of Clinical Chemistry, University Hospital, S-751 85 Uppsala, Sweden

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

We have previously shown that porcine aortic endothelial cells expressing the Y934F platelet-derived growth factor (PDGF) -receptor mutant respond to PDGF-BB in a chemotaxis assay at about 100-fold lower concentration than do wild-type PDGF -receptor-expressing cells (Hansen, K., Johnell, M., Siegbahn, A., Rorsman, C., Engström, U., Wernstedt, C., Heldin, C.-H., and Rönnstrand, L. (1996) EMBO J. 15, 5299-5313). Here we show that the increased chemotaxis correlates with increased activation of phospholipase C-1 (PLC-1), measured as inositol-1,4,5-trisphosphate release. By two-dimensional phosphopeptide mapping, the increase in phosphorylation of PLC-1 was shown not to be selective for any site, rather a general increase in phosphorylation of PLC-1 was seen. Specific inhibitors of protein kinase C, bisindolylmaleimide (GF109203X), and phosphatidylinositol 3-kinase (PI3-kinase), LY294002, did not affect the activation of PLC-1. To assess whether increased activation of PLC-1 is the cause of the hyperchemotactic behavior of the Y934F mutant cell line, we constructed cell lines expressing either wild-type or a catalytically compromised version of PLC-1 under a tetracycline-inducible promoter. Overexpression and concomitant increased activation of wild-type PLC-1 in response to PDGF-BB led to a hyperchemotactic behavior of the cells, while the catalytically compromised PLC-1 mutant had no effect on PDGF-BB-induced chemotaxis. Furthermore, in cells expressing normal levels of PLC-1, chemotaxis was inhibited by LY294002. In contrast, the increase in chemotactic response seen upon overexpression of PLC-1 was not inhibited by the PI3-kinase inhibitor LY294002. These observations suggest the existence of two different pathways which mediate PDGF-induced chemotaxis; depending on the cellular context, the PI3-kinase pathway or the PLC-1 pathway may dominate.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Platelet-derived growth factor (PDGF)¹ stimulation of responsive cells leads to induction of mitogenicity, chemotaxis, and actin reorganization (for review, see Ref. 1). PDGF is a family of dimeric isoforms consisting of different combinations of disulfide-bonded A- and B-chains. Thus, three isoforms of PDGF exist with distinct binding characteristics toward the structurally related PDGF - and -receptors. Binding of PDGF to its receptors leads to dimerization of the receptors, an essential event in PDGF receptor activation (2, 3). Dimerization leads to autophosphorylation on a number of tyrosine residues in the intracellular part of the receptors, providing docking sites for a class of signal transduction molecules containing Src homology 2 (SH2) domains, including members of the Src family of tyrosine kinases, phosphatidylinositol 3-kinase (PI3-kinase), the GTPase activating protein of Ras (GAP), and phospholipase C-1 (PLC-1) (1).

In addition to undergoing autophosphorylation, the PDGF -receptor is also phosphorylated on one specific tyrosine residue by members of the Src family of tyrosine kinases. Autophosphorylation of the PDGF -receptor in the juxtamembrane region leads to association, phosphorylation, and activation of c-Src (4) and to a subsequent phosphorylation by Src of Tyr⁹³⁴ in the receptor (5). Mutation of Tyr⁹³⁴ to a phenylalanine residue and expression of the mutant receptor in porcine aortic endothelial (PAE) cells revealed an increased chemotactic response compared with cells expressing the wild-type PDGF -receptor (5).

Blocking the association of the p85 subunit of PI3-kinase with the PDGF -receptor, through mutation of Tyr⁷⁴⁰ and Tyr⁷⁵¹ to phenylalanine residues, led to a significant reduction of the chemotactic response in response to PDGF-BB (6), which together with the observation that PDGF-induced chemotaxis is strongly inhibited by the PI3-kinase inhibitor LY294002 (5) suggests a role for PI3-kinase in mediating a chemotactic response to PDGF-BB.

The present study was undertaken to investigate the mechanisms behind the increased chemotactic response in the Y934F mutant cell line. We present data here that phosphorylation and activation of PLC-1 are considerably increased in the Y934F mutant receptor cell line. Furthermore, by the use of cell lines expressing either wild-type or a catalytically compromised mutant of PLC-1 under the tetracycline-inducible promoter, we could show that the lipase activity of PLC-1 is essential for the hyperchemotactic response and that cells overexpressing PLC-1 are independent of PI3-kinase for PDGF-induced chemotaxis.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Materials-- Precoated cellulose thin layer chromatography plates were purchased from Merck (Darmstadt, Germany), modified sequencing grade trypsin from Promega (Madison, WI). Polyvinylpyrrolidone was from Aldrich (Steinheim, Germany). Radionuclides were from Amersham Pharmacia Biotech (Buckinghamshire, UK). The Hunter thin layer electrophoresis system was from C.B.S. Scientific Co. (Del Mar, CA). LY294002 was from Biomol (Plymouth Meeting, PA). Rabbit antiserum against PLC-1 was generated by immunizing rabbits with a peptide corresponding to the carboxyl terminus of bovine PLC-1 (7). An antiserum against the hemagglutinin (HA) epitope tag was raised by immunizing a rabbit with the synthetic peptide YPYDVPDYAGYPYDVPDYA conjugated to keyhole limpet hemocyanin (KLH).

Construction of Cell Lines Containing PLC-1 under a Tetracycline-inducible Promoter-- PAE cells expressing the PDGF -receptor were stably transfected by electroporation with the regulatory plasmid pUHD 172-1-neo (8). G-418-resistant clones were screened by transient transfection with the luciferase reporter plasmid pUHC 13-3 and tested for luciferase activity in the presence or absence of doxycycline. Clones with low background expression of luciferase and high inducibility in the presence of doxycycline were chosen for transfection with the reporter plasmid pUHD 10-3 containing either wild-type PLC-1 or a lipase inactive PLC-1 (H335F/H380F), each containing a carboxyl-terminal HA-tag. Plasmids were co-transfected with pPKGpuro, providing puromycin as selection marker. Puromycin-resistant clones were screened by Western blotting for expression of PLC-1 in the presence or absence of doxycycline, using either anti-PLC-1 antiserum or an anti-HA-tag antiserum. The clone used expressing wild-type PDGF -receptor and wild-type PLC-1 is thus denoted PAE/wt/PLC-1_wt, whereas the clone expressing wild-type PDGF--receptor and lipase compromised PLC-1 is denoted PAE/wt/PLC-1_mut.

Assay for Release of Inositol Phosphates-- Six-well plates with semi-confluent cultures of PAE/wt/PLC-1_wt or PAE/wt/PLC-1_mut cells were incubated in the presence or absence of 20 ng/ml doxycycline for 24 h to induce protein expression, followed by overnight incubation with 2 µCi of myo-[³H]inositol in 2 ml of inositol-free Ham's F-12, containing 0.3% fetal calf serum. In cases where bisindolylmaleimide or LY294002 were used, inhibitors were added to the medium 60 min before stimulation with PDGF-BB. Assay for PDGF-induced release of inositol phosphates was performed essentially according to Eriksson et al. (9).

In vivo [³²P]Orthophosphate Labeling of Cells-- Cells were seeded at subconfluency 2 days before the experiment and starved for 24 h in 0.2% fetal calf serum. Cells were labeled with [³²P]orthophosphate essentially as described by Hansen et al. (5).

In Situ Trypsin Digestion, Two-dimensional Phosphopeptide Mapping, and Phosphoamino Acid Analysis-- Phosphorylated proteins were separated by SDS-polyacrylamide gel electrophoresis, using an 8% gel and electrotransferred to nitrocellulose membrane (Hybond-C Extra, Amersham Pharmacia Biotech). Samples were processed for tryptic digestion, two-dimensional phosphopeptide mapping, and phosphoamino acid analysis, as described by Blume-Jensen et al. (10).

Cell Motility Assay-- The chemotactic response of the different cell lines was assayed by means of the leading front technique in a modified Boyden chamber, as described previously (11). In experiments where the effects of different inhibitors on the motility response were tested, the cells were preincubated for 10 min at 37 °C with the inhibitor at the indicated concentrations before the experiment. The inhibitors were present throughout the experiment. In cases when doxycycline-induced protein expression was used, cells were incubated with 10 ng/ml doxycycline for 24 h before the chemotaxis assay was performed. The chemotaxis assays were performed in Ham's F-12, supplemented with 10% fetal calf serum and antibiotics.

Mitogenicity Assay-- PAE/wt/PLC-1_wt and PAE/wt/PLC-1_mut cells were grown to confluency in 12-well plates and starved for 24 h in Ham's F-12 containing 1 mg/ml bovine serum albumin, in the presence or absence of 20 ng/ml doxycycline to induce protein expression. Medium was changed to the above medium containing 0.2 µg/ml [³H]thymidine and varying concentrations of PDGF-BB. Cells were incubated for 24 h at 37 °C, after which cells were fixed with trichloroacetic acid and precipitated radioactivity was quantitated in a scintillation counter.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Analysis of PDGF-BB-stimulated Phosphorylation of PLC-1-- We have previously shown that cells expressing a PDGF -receptor with Tyr⁹³⁴ mutated to a phenylalanine residue, show enhanced chemotactic response compared with wild-type receptor-expressing cells (5). It was also demonstrated that tyrosine phosphorylation of PLC-1 in response to PDGF-BB was increased in cells expressing the Y934F mutant receptor. To assess whether the increase in phosphorylation was selective for a particular tyrosine residue, and whether additional phosphorylation sites appeared in cells expressing the Y934F mutant, cells expressing the wild-type PDGF -receptor and the Y934F mutant receptor were labeled with [³²P]orthophosphate. Following stimulation with PDGF-BB, cells were lysed and PLC-1 immunoprecipitated and processed for two-dimensional tryptic phosphopeptide mapping. A general increase in the intensity of peptides phosphorylated in response to PDGF-BB could be seen in the Y934F mutant cell line, compared with cells expressing the wild-type PDGF -receptor (Fig. 1). Several peptides containing phosphotyrosine, as well as phosphoserine, appeared in response to PDGF-BB stimulation (data not shown). No selective increase in the phosphorylation of any peptide, nor any new phosphopeptides, were seen in PLC-1 from Y934F mutant cells.

View larger version (29K): [in this window] [in a new window]   Fig. 1.   Two-dimensional tryptic phosphopeptide maps of PLC-1 from wild-type PDGF -receptor-expressing PAE cells or cells expressing the Y934F mutant PDGF -receptor. Cells were labeled with [32P]orthophosphate, stimulated with PDGF-BB or left unstimulated, lysed, and immunoprecipitated with an anti-PLC-1 antiserum. Immunoprecipitated protein was separated by SDS-gel electrophoresis, electrotransferred to Hybond-C Extra, and digested in situ with trypsin. Phosphopeptides were separated by electrophoresis at pH 1.9 in the first dimension and by chromatography using isobutyric acid chromatography buffer in the second dimension. A, PAE/wt cells without PDGF-BB stimulation; B, PAE/wt cells with PDGF-BB stimulation; C, PAE/Y934F cells without PDGF-BB stimulation; and D, PAE/Y934F cells with PDGF-BB stimulation.

Independence of PKC and PI3-Kinase for PLC-1 Activity-- Previously, we have shown that the increased chemotactic response seen in the Y934F mutant receptor cell line could be inhibited by bisindolylmaleimide, a protein kinase C inhibitor, while leaving the wild-type receptor-induced chemotactic response virtually unaffected. In contrast, an inhibitor of PI3-kinase, LY294002, effectively inhibited the chemotactic response seen in wild-type receptor-expressing cells, with little effect on the chemotactic response elicited by the Y934F mutant receptor (5). Therefore, we investigated the effect of bisindolylmaleimide and LY294002 on PLC-1 activity in the two cell lines. Cells were labeled with [³H]myoinositol, incubated with the respective inhibitors, followed by stimulation with PDGF-BB and analysis of produced IP₃ (Fig. 2). The inhibitor of protein kinase C, bisindolylmaleimide, showed no effect on PLC-1 activity, neither in wild-type receptor cells nor in the Y934F mutant cells, whereas the PI3-kinase inhibitor LY294002 slightly inhibited the PLC-1 activity in both cell lines.

View larger version (31K): [in this window] [in a new window]   Fig. 2.   Effect of LY294002 and GF109203X on PDGF-BB stimulated inositol phosphate formation in wild-type PDGF -receptor-expressing cells and in cells expressing the Y934F mutant PDGF -receptor. Cells were labeled with myo-[3H]inositol overnight and stimulated with PDGF-BB. Released inositol phosphates were determined. Cells were either treated with Me2SO (control), 20 nM GF109203X (GF), or 2.8 µM LY294002 (LY)

Establishment of Stable Cell Lines Containing Wild-type or Dominant Negative PLC-1 under the Control of an Inducible Promoter-- In order to investigate whether increased PLC-1 activity causes increased chemotaxis and thus could explain the hyperchemotactic phenotype of the Y934F mutant cell line, wild-type PDGF -receptor-expressing cells were transfected with wild-type or catalytically compromised HA-tagged human PLC-1 under the control of a tetracycline-inducible promoter. Cell clones were analyzed for expression of PLC-1 in the presence or absence of doxycycline. Conditions were optimized so that the expression level of PLC-1 would lead to a response in IP₃ release after PDGF-BB stimulation similar to that seen in the Y934F cells (Fig. 3). The clones used expressed a negligible amount of HA-tagged protein in the absence of doxycycline, while the protein expression was dramatically induced by doxycycline (Fig. 4).

View larger version (16K): [in this window] [in a new window]   Fig. 3.   Effect of overexpression of wild-type PLC-1 and a lipase-inactive mutant of PLC-1 on PDGF-BB-stimulated inositol trisphosphate release. Cells induced to express either PLC-1 construct by induction with 10 ng/ml doxycycline for 24 h, or non-induced control, were labeled overnight with myo-[3H]inositol in the presence or absence of 10 ng/ml doxycycline. Cells were subsequently stimulated with PDGF-BB and analyzed for released inositol phosphates.

View larger version (17K): [in this window] [in a new window]   Fig. 4.   Doxycycline induced expression of PLC-1 in the PAE/wt/PLC-1wt and PAE/wt/PLC-1mut cell lines. Cells were left untreated or induced to express proteins with 10 ng/ml doxycycline for 24 h. Cell lysates were separated by SDS-gel electrophoresis, followed by electrotransfer to Immobilon-P filters. Filters were probed with either anti-PLC-1 antiserum or anti-HA antiserum. IB, immunoblotting.

Overexpression of PLC-1 Leads to a Hyperchemotactic Response to PDGF-BB-- Expression of PLC-1 was induced with doxycycline for 24 h. Cells were then assayed for chemotaxis by means of the leading front technique in a modified Boyden chamber. In accordance with the Y934F mutant cells, PDGF -receptor cells overexpressing wild-type PLC-1 responded to PDGF-BB with chemotaxis at concentrations almost 100-fold lower compared with control cells without induction of PLC-1 expression (Fig. 5A). In contrast, cells overexpressing a similar amount of a catalytically compromised version of PLC-1, in which two histidine residues (His³³⁵ and His³⁸⁰) in the catalytic domain had been mutated to phenylalanine residues, had exactly the same chemotactic response as control cells (Fig. 5B).

View larger version (22K): [in this window] [in a new window]   Fig. 5.   Effect of overexpression of PLC-1 on chemotaxis. PAE/wt/PLC-1wt or PAE/wt/PLC-1mut cells were treated with 10 ng/ml doxycycline for 24 h. Chemotaxis toward PDGF-BB was assayed using the leading front technique in a modified Boyden chamber. A, PAE/wt cells with () or without () doxycycline; PAE/wt/PLC-1wt cells with () or without () doxycycline; B, PAE/wt/PLC-1mut cells with () or without () doxycycline.

The Chemotactic Response in Cells Overexpressing PLC-1 Is Resistant to Inhibition by LY294002-- Chemotaxis induced by PDGF-BB in wild-type PDGF -receptor-expressing PAE cells is efficiently inhibited by LY294002 (1). Similarly, chemotaxis was inhibited by LY294002 in the absence of doxycycline in cells expressing wild-type PLC-1 under a tetracycline-inducible promoter. In contrast, when cells were incubated with doxycycline to induce overexpression of PLC-1, the PI3-kinase inhibitor LY294002 had no effect on PDGF-BB-induced chemotaxis (Fig. 6A). In contrast, when cells expressing a dominant negative H335F/H380F mutant of PLC-1 under a tetracycline-inducible promoter were incubated with doxycycline, the PI3-kinase inhibitor LY294002 effectively inhibited PDGF-BB-induced chemotaxis (Fig. 6B). Thus, the lipase activity of PLC-1 is necessary to allow cells to migrate in the presence of PI3-kinase inhibitor.

View larger version (17K): [in this window] [in a new window]   Fig. 6.   Effect of inhibition of PI3-kinase on chemotaxis in cells overexpressing PLC-1. PAE/wt/PLC-1wt cells (A) or PAE/wt/PLC-1mut cells (B) were incubated either in the presence () or absence () of 10 ng/ml doxycycline for 24 h. Cells were incubated with varying concentrations of LY294002 for 30 min at 37 °C prior to and during the chemotaxis assay. Chemotaxis was induced by 10 ng/ml PDGF-BB.

Overexpression of Either Wild-type or Dominant Negative PLC-1 Has No Effect on PDGF-BB-induced Mitogenicity in PDGF -Receptor-expressing PAE Cells-- In order to assess the possible role of PLC-1 in mediating the mitogenic response to PDGF-BB, cells were incubated with doxycycline for 24 h to induce overexpression of either wild-type PLC-1 or catalytically compromised PLC-1, followed by stimulation with varying concentrations of PDGF-BB and incubation with [³H]thymidine for 24 h. However, no effect was seen on PDGF-BB-induced [³H]thymidine incorporation in cells expressing the wild-type or the catalytically compromised PLC-1 (Fig. 7, A and B).

View larger version (16K): [in this window] [in a new window]   Fig. 7.   Effect of PLC-1 overexpression on mitogenicity induced by PDGF-BB in wild-type PDGF -receptor-expressing cells. Cells were incubated in the presence or absence of doxycycline for 24 h, followed by a 24-h incubation with [3H]thymidine in the presence of varying concentrations of PDGF-BB, with or without doxycycline present. Labeled DNA was precipitated by trichloroacetic acid, and the incorporation of [3H]thymidine into DNA was measured in a scintillation counter. A, PAE/wt/PLC-1wt cells with () or without () doxycycline. B, PAE/wt/PLC-1mut cells with () or without () doxycycline. FBS, fetal bovine serum.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

We have previously shown that c-Src phosphorylates Tyr⁹³⁴ in the second part of the kinase domain of the PDGF -receptor (5). Cells transfected with a PDGF -receptor mutant with Tyr⁹³⁴ replaced with a phenylalanine residue showed an increased tyrosine phosphorylation of PLC-1 and an enhanced chemotactic response to PDGF-BB. We have now further shown that the enhanced chemotaxis coincides with an increase in phosphorylation of PLC-1 on the same sites as those phosphorylated in response to activation of the wild-type PDGF -receptor. Kim et al. (12) have shown that phosphorylation of Tyr⁷⁷¹ and Tyr⁷⁸³ in PLC-1 are essential for activation. No change in the stoichiometry in phosphorylation of Tyr¹⁰²¹, the primary association site for PLC-1 in the PDGF -receptor, was seen in cells expressing the Y934F mutant compared with cells expressing wild-type PDGF -receptor (data not shown). The reason for the increased phosphorylation of PLC-1 in cells expressing the Y934F mutant PDGF -receptor is not known; it is possible that the receptor kinase in the Y934F mutant has altered kinetics toward PLC-1 as a substrate. An additional possibility is that the increase in phosphorylation of PLC-1, at least in part, could be due to the action of c-Src since it has been shown that c-Src is able to associate with and phosphorylate PLC-1 (13, 14). We found that the increased phosphorylation of PLC-1 correlated with an increase in IP₃ production (Fig. 2). The magnitude of IP₃ production produced in the Y934F mutant cell line was about 3-fold higher than in wild-type receptor-expressing cells. The difference in response is not due to differences in receptor number or ligand affinity between the cell lines, as indicated by Scatchard analyses of PDGF-BB binding (1).

The importance of PLC-1 in chemotactic signaling was pointed out by Kundra et al. (15, 16). However, PAE cells expressing a PDGF -receptor mutant, with the association site for PLC-1 Tyr¹⁰²¹ mutated to a phenylalanine residue, showed unaffected chemotaxis toward the ligand (6). On the other hand, Wennström et al. (6) demonstrated that inhibition of PI3-kinase with wortmannin in PAE cells expressing PDGF -receptors led to inhibited chemotaxis. In contrast, Higaki et al. (17) showed that, in vascular smooth muscle cells and Swiss 3T3 cells, inhibition of PI3-kinase had no effect on chemotaxis. The chemotactic response seen in the Y934F mutant cell line was, in contrast to the response seen in the wild-type PDGF -receptor-expressing cells, inhibited by bisindolylmaleimide, an inhibitor of protein kinase C, but resistant to inhibition by the PI3-kinase inhibitor LY294002 (1). Here we demonstrate that overexpression and enhanced activation of PLC-1 also results in enhanced chemotactic response and that, under these conditions, the PI3-kinase inhibitor LY294002 has no effect on the chemotaxis induced by PDGF-BB. Taken together, these data suggest that at least two pathways can mediate PDGF -receptor-induced chemotaxis, i.e. PI3-kinase and PLC-1. It is possible that the expression levels of PI3-kinase and PLC-1, and their magnitude of activation, determines which of these pathways will dominate.

Activation of PLC-1 leads to production of two second messengers, IP₃ and diacylglycerol (DAG). Binding of IP₃ to receptors on the endoplasmic reticulum leads to release of calcium, while DAG is an activator of the classical PKCs (18). Furthermore, DAG has been implicated in stimulation of chemotaxis in lymphocytes (19, 20). We could not detect any effect of the PKC inhibitor bisindolylmaleimide on PLC-1 activity, neither in wild-type cells, nor in the mutant cell line (Fig. 2). This is in contrast to findings by Ozawa et al. (21), who described feedback inhibition of PLC-1 by PKC- and PKC- in rat basophil RBL-2H3 cells. The PI3-kinase inhibitor, LY294002, had a slight reducing effect on PLC-1 activity. This is consistent with findings that full activation of PLC-1 requires binding of phosphatidylinositol-3,4,5-trisphosphate to its pleckstrin homology (PH) domain (22, 23), an event that triggers translocation to the membrane. In conclusion, the inhibitors bisindolylmaleimide and LY294002 are likely to act downstream rather than upstream of PLC-1 in the signaling pathways leading to chemotaxis.

The essential role of PLC-1 in mammalian growth and development was shown in mice with a targeted deletion of the PLC-1 locus. This deletion results in embryonic lethality at approximately day 9.0 of embryonic development (24). The exact role of PLC-1 in mitogenesis is debated. Smith et al. (28) and Huang et al. (29) showed that microinjection of wild-type as well as the catalytically compromised mutant of PLC-1 resulted in mitogenesis, suggesting that domains other than the catalytic domain might be important for signaling. By microinjection of different domains of PLC-1 into NIH3T3 cells, Smith et al. (25) showed that either the SH3 domain or the SH2 domains induced a partial response that was restored when they were co-injected. The PH domain, however, did not induce DNA synthesis. In contrast, using the same cells, Huang et al. (26) showed that deletion of the SH3 domain resulted in complete loss of mitogenic response. Using MDCK epithelial cells and NIH3T3 cells, Wang et al. (27) could block PDGF-induced S-phase entry by microinjection of a polypeptide comprising the two SH2 domains and the SH3 domain of PLC-1. Microinjection of DAG and IP₃ did not stimulate DNA synthesis by themselves but did suppress the inhibitory effect of the SH2-SH2-SH3 polypeptide. These observations suggest that both the SH2 and SH3 domains and the catalytic activity of PLC-1 are important for mediation of a mitogenic response. In this report we did not observe any effect on PDGF-BB-induced DNA replication upon overexpression of either wild-type PLC-1 or a catalytically compromised version of the lipase.

In summary, we have shown that either PI3-kinase or PLC-1 are required for induction of chemotaxis by the PDGF -receptor. Our findings suggest that it is the expression levels of these individual enzymes and the extent of their activation that determines which pathway that mediates the chemotactic response in individual cell lines. It is possible that the PLC-1 and the PI3-kinase pathways are both needed for the chemotactic response, or one can substitute for the other given that it is expressed and activated at sufficient levels. Cross-talk between the PLC-1 pathway and PI3-kinase pathway is known on several levels. Full activation of PLC-1 was shown to require the activity of PI3-kinase (22, 23, 28). Furthermore, Derman et al. could show that products of PI3-kinase increased cell motility through PKC (29). In addition to being activated by DAG, a product of PLC-1, PKC isoforms have been shown to be phosphorylated and activated by PDK1, a downstream target of PI3-kinase (30). Phosphorylation of the same conserved threonine residue in the activation loop has previously been shown to be essential to render PKC catalytically competent to autophosphorylate (31). Furthermore, PKC- has been shown to be activated by both PI3-kinase and PLC-1-dependent pathways (32). However, one cannot exclude the possibility that IP₃ produced by PLC-1, leading to release of calcium from internal stores, plays a role in the increased chemotactic response seen upon PLC-1 overexpression. An important future goal will be to identify the common downstream signaling molecules that mediate the chemotactic response seen after activation of PI3-kinase or PLC-1.

	ACKNOWLEDGEMENT

We thank Dr. Klaus Seedorf at the Hagedorn Research Institute, Copenhagen, for cDNAs encoding wild-type PLC-1 and the lipase-deficient PLC-1 mutant.

	FOOTNOTES

^* This work was supported in part by a grant from the Nordic Cancer Union, the Swedish Cancer Society, and the Axel and Margaret Ax:son Johnson Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: +46 18 16 0406; Fax: +46 18 16 04 20; E-mail: Lars.Ronnstrand@LICR.uu.se.

	ABBREVIATIONS

The abbreviations used are: PDGF, platelet-derived growth factor; DAG, diacylglycerol; HA, hemagglutinin epitope; IP₃, inositol-1,4,5-trisphosphate; PAE, porcine aortic endothelial; PH, pleckstrin homology; PI3-kinase, phosphatidylinositol 3-kinase; PLC-1, phospholipase C-1; SH2, Src homology 2; SH3, Src homology 3; PKC, protein kinase C.

	REFERENCES

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