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J. Biol. Chem., Vol. 281, Issue 37, 27405-27415, September 15, 2006

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Calcium-regulated Phosphorylation of Soybean Serine Acetyltransferase in Response to Oxidative Stress^*

Fenglong Liu¹, Byung-Chun Yoo², Jung-Youn Lee²³, Wei Pan, and Alice C. Harmon⁴

From the Program in Plant Molecular and Cellular Biology and the Department of Botany, University of Florida, Gainesville, Florida 32611-8526 and the Department of Plant and Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711

Received for publication, May 11, 2006 , and in revised form, July 17, 2006.

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
Glycine max serine acetyltransferase 2;1 (GmSerat2;1) is a member of a family of enzymes that catalyze the first reaction in the biosynthesis of cysteine from serine. It was identified by interaction cloning as a protein that binds to calcium-dependent protein kinase. In vitro phosphorylation assays showed that GmSerat2;1, but not GmSerat2;1 mutants (S378A or S378D), were phosphorylated by soybean calcium-dependent protein kinase isoforms. Recombinant GmSerat2;1 was also phosphorylated by soybean cell extract in a Ca²⁺-dependent manner. Phosphorylation of recombinant GmSerat2;1 had no effect on its catalytic activity but rendered the enzyme insensitive to the feedback inhibition by cysteine. In transient expression analyses, fluorescently tagged GmSerat2;1 localized in the cytoplasm and with plastids. Phosphorylation state-specific antibodies showed that an increase in GmSerat2;1 phosphorylation occurred in vivo within 5 min of treatment of soybean cells with 0.5 mM hydrogen peroxide, whereas GmSerat2;1 protein synthesis was not significantly induced until 1 h after oxidant challenge. Internal Ca²⁺ was required in the induction of both GmSerat2;1 phosphorylation and synthesis. Treatment of cells with calcium antagonists showed that externally derived Ca²⁺ was important for retaining GmSerat2;1 at a basal level of phosphorylation but was not necessary for its hydrogen peroxide-induced synthesis. Protein phosphatase type 1, but not type 2A or alkaline phosphatase, dephosphorylated native GmSerat2;1 in vitro. These results support the hypothesis that GmSerat2;1 is regulated by calcium-dependent protein kinase phosphorylation in vivo and suggest that increased GmSerat2;1 synthesis and phosphorylation in response to active oxygen species could play a role in anti-oxidative stress response.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
In plants and microorganisms, cysteine is the point of entry for inorganic sulfur into organic compounds. Cysteine synthesis proceeds via two steps: 1) L-serine + acetyl-CoA O-acetylserine + CoA and 2) O-acetylserine + S^2- L-cysteine + acetate. In the first step, serine acetyltransferase (SAT,⁵ EC 2.3.1.30 [EC] ) transfers an acetyl group from acetyl-CoA to serine to form the intermediate product, O-acetylserine (OAS). In the next reaction, catalyzed by O-acetylserine (thiol)-lyase (OAS-TL, EC 4.2.99.8 [EC] ), sulfide reacts with OAS, forming cysteine.

In plants, SAT and OAS-TL are found in multiple subcellular compartments, and the activity of SAT in these compartments is 4-300-fold less than that of OAS-TL (1-3). The availability of OAS has been found to be limiting for cysteine biosynthesis (4, 5), and SAT is in a position to regulate not only cysteine synthesis but also sulfur homeostasis for the whole plant (6). SAT activity is thought to be regulated by formation of a complex with OAS-TL (3, 7), and some forms of plant SATs are feedback-inhibited by cysteine. In Arabidopsis, two of the three cytosolic SATs are inhibited by cysteine, while the plastidic and mitochondrial SATs are not (8, 9). The cysteine-sensitive SAT activity of pea is in the plastid rather than in the cytosol (2). The variation among species in the sensitivity of plastidic and cytosolic SATs to cysteine suggests variation in the regulation of cysteine synthesis from species to species and compartment to compartment. Species differences in other parts of the sulfur assimilation pathway (10) further emphasize variation in sulfur metabolism and its regulation in plants.

Glutathione (-Glu-Cys-Gly) or homoglutathione (-GluCys--Ala) in legumes, including soybean, protects plants from oxidative damage that arises from various stress conditions. Considerable increases in the pool size of glutathione have been reported in plants exposed to various abiotic and biotic stresses such as chilling (11), drought (12), herbicide safeners (13), and pathogen attack (14). Increased glutathione concentrations were observed when cysteine content was increased artificially by supplying sulfate (15), fumigating with H₂S (16, 17), direct feeding of cysteine (18, 19), or overexpressing SAT (20), suggesting cysteine availability plays an important role in determining glutathione content. Recent evidence shows that in addition to increasing glutathione, stresses such as heat shock, chilling, hypoosmolarity, and exogenous application of H₂O₂ trigger increases in reactive oxygen species and cytosolic calcium in plant cells and seedlings (21-26) and that calcium is necessary for the stress response.

In this paper we show that a serine acetyltransferase from soybean (GmSerat2;1) is a substrate of CDPK (calcium-dependent protein kinase). CDPKs function as both Ca²⁺ sensors and effectors (27). Phosphorylation of GmSerat2;1 at a specific serine residue in the carboxyl terminus renders the enzyme insensitive to feedback inhibition by cysteine. Using phosphorylation state-specific antibodies, we show that GmSerat2;1 is phosphorylated in soybean cells. Furthermore, treatment of cells with H₂O₂ induces both phosphorylation and protein synthesis of GmSerat2;1, and internally derived calcium and kinase activity are involved in the induction. Based on these results we propose that H₂O₂-induced increase in the activity of GmSerat2;1 contributes to the oxidative stress response in soybean.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
Plant Material—Soybean cell suspension cultures (Glycine max L.) were maintained as described previously (28). Cells were subcultured every 7 days, and all treatments were done using cells 3 days after transfer to fresh medium. Inhibitors and H₂O₂ were added to the indicated concentrations. Following incubation for the indicated times, cells were collected on Miracloth (EMD Biosciences, San Diego, CA), drained of culture medium, and washed with 20 mM Tris, pH 8.5, 0.4 M sorbitol, 10 mM MgCl₂, 1 mM NaF, 1 mM Na₃VO₄, and 1 mM Na₂MO₄.

DNA Sequencing—Sequences of all constructs were verified by DNA sequencing performed by the University of Florida DNA Sequencing Core Facility. The sequence for GmSerat2; 1N (IP3) has been deposited in the GenBank^TM data bank under the accession number AY422685 [GenBank] .

Interaction Cloning—Procedures for interaction cloning (identification of proteins expressed from a cDNA library that bind to a labeled probe) were adapted from Blanar and Rutter (29) and Stone et al. (30). A soybean cDNA library made in the UniZap^TM XR vector, which allows expression of cDNA inserts as fusion proteins with -galactosidase, was the gift of R. Tenhaken and C. Lamb. Plating of phage and expression and transfer of proteins to nitrocellulose membranes were performed using a picoBlue^TM immunoscreening kit (Stratagene, La Jolla, CA). Nitrocellulose membranes were washed in 20 mM Tris, pH 7.5, 150 mM NaCl, and 0.05% Tween 20 three times at 25 °C and incubated in 25 mM Tris, pH 7.5, 150 mM NaCl, 0.5 mM DTT, 5 mM MgCl₂, and 5% (w/v) nonfat dry milk for 1-2 h at 4 °C. Probes were purified recombinant soybean CDPK,-, and - labeled with ³²P by autophosphorylation in 100 µl of phosphorylation buffer (31) for 15 min at 25 °C. After autophosphorylation and removal of unincorporated [-³²P]ATP by gel filtration, probes were equilibrated with binding buffer (25 mM HEPES, pH 7.5, 10 mM MgCl₂, 1 mM EGTA, 1.2 mM CaCl₂, 2 mM DTT, and 50 mM NaCl), and the concentration adjusted to 2-5 x 10⁵ cpm/ml. Hybridization was performed at 4 °C overnight with continuous shaking in binding buffer supplemented with 1% nonfat dry milk. Membranes were washed three times for 10 min each at 25 °C in the same buffer, air-dried, and exposed to x-ray film.

Complementation Assay—Escherichia coli strain JM39 (F⁺, cysE51, recA56), which lacks serine acetyltransferase activity and requires L-cystine for growth (32), was transformed with putative SAT clones and tested for complementation by growth on mediim lacking L-cystine.

Plasmid Construction and Site-directed Mutagenesis—To make a construct encoding maltose-binding protein (MBP) fused to the NH₂ terminus of GmSerat2;1N, the open reading frame of clone IP3, which encoded interacting protein 3, was PCR-amplified and cloned into pMal-cRI (New England Biolabs, Ipswich, MA). The 5' primer was 5'-GCGGATCCCATATGGCCACTTGTGTT (BamHI, NdeI, underlined) and 3' primer was 5'-GCGGATCCCTATATAACATAATC (BamHI). The BamHI-digested fragment was ligated into pMal-cRI. The integrity of subcloning was verified by complementation assay as described above. The point mutation (S378A) was made by recombination PCR (33). pBluescript KS/GmSerat2;1N, constructed by subcloning BamHI-digested pMal-cRI/GmSerat2;1N into pBluescript KS, was linearized and used as a template for PCR with mutagenic primers: primer 1, 5'-GATGCCTga/cTTTTACtATGGACCATACTTCATGGTCTGATTA-3'; primer 2, 5'-CATAATCAGACCATGAAGTATGGTCCATaGTAAAAg/tcAGGCATCTTATCC-3'; primer 3 (2266-2286), 5'-TGCGCAACGTTGTTGCCATTG-3'; primer 4 (2283-2260), 5'-TGGCAACAACGTTGCGCAAACTAT-3'. The resultant plasmid, pBluescript KS/GmSerat2;1(S378A), was digested with BamHI and ligated into pMal-cRI.

A GmSerat2;1 cDNA containing a full-length open reading frame was cloned by reverse transcriptase-PCR using total RNA prepared from soybean culture cells and gene-specific primers. PCR products were cloned into either pdGN (34) for expression of carboxyl-terminal GFP fusion proteins or pdYN⁶ for expression of COOH-terminal YFP fusion proteins under the control of the cauliflower mosaic virus 35S promoter. For construction of pMal-cRI/GmSerat2;1, PCR was performed with primers YooP1 (5'-CGGAATTCATGAATGTTCTGGCTCTAGGGCG-3') and YooP2 (5'-CGGGATCCTATATAACATAATCAGACCATG-3') and pdGN/GmSerat2;1 as template.

GmSerat2;1(S378D) was constructed by PCR-based mutagenesis with primers YooP1, YooP4 (5'-GACCATGAAGTATGGTCCATGATAAAATCAGGCATCTTATCCAATTTAATAGGG-3') and pBluescriptSK-GmSerat2;1 as a template. The PCR product was the template in a second PCR reaction with primers YooP1 and YooP9 (5'-GTATGGTCCATGGTAAAATCAGGCATC-3'). An NcoI-digested fragment was subsequently isolated and ligated into NcoI-digested pBluescriptSK-GmSerat2;1.

Expression and Purification of Fusion Proteins—Protocols for expression of recombinant proteins were described previously (35). For purification of MBP fusion proteins, bacterial cells were resuspended in extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM KCl, 10 mM EDTA, 20 µg/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride, and 10 µg/ml leupeptin) and lysed by sonication. After clarification by centrifugation at 10,000 x g for 20 min, the supernatant was passed through a 0.45-µm filter and loaded onto a column of amylose-agarose (New England Biolabs) equilibrated with a binding buffer (50 mM Tris-HCl, pH 7.5, 150 mM KCl). After extensive washing with binding buffer, proteins were eluted with 10 mM maltose in binding buffer. Purified proteins were stored in 50% glycerol at -80 °C. The activity of MBP-GmSerat2;1 was stable at room temperature for several days, and small losses of activity (10-20%) were observed after several cycles of freezing and thawing. Inclusion of DTT and glycerol in the purification buffers did not affect yield but appeared to help preserve activity during freezing and thawing. Expression and purification of His₆-CDPK was described previously (31).

Protein Kinase Assay—CDPK activity assays were performed as described previously (31).

Phosphorylation of Serine Acetyltransferase—Phosphorylation of SAT was performed on ice in 50 mM HEPES, pH 7.2, 10 mM MgCl₂, 1 mM EGTA, 1.2 mM CaCl₂ (omitted for calciumfree reactions), 1 mM DTT, 0.05 mg/ml bovine serum albumin, 1 mM [³²P]ATP (500 cpm/pmol), and 4 ng of His₆-CDPK for every 1 µg of MBP fusion protein. The reactions were initiated by addition of ATP and stopped by the addition of 4 volumes of stop buffer (50 mM HEPES, pH 7.2, 10 mM EDTA, 5 mM EGTA). Aliquots of reactions were resolved on SDS-PAGE, and dried gels were exposed to x-ray film. To determine phosphorylation stoichiometry (moles of ³²P incorporated per mole of protein) protein bands were excised from gels, and radioactivity was measured by liquid scintillation counting. To test the effect of phosphorylation on SAT activity, phosphorylation reactions were carried out as described above except without radioisotope, and aliquots were used to measure SAT activity.

Activity Assays of Serine Acetyltransferase—The radiometric assay was carried out in a 100-µl mixture that contained 50 mM Tris-HCl, pH 7.5, 1 mM acetyl-CoA, and 1 mM [³H]L-serine (1 µCi), with or without 1 mM L-cysteine, and with indicated amounts of SAT, either untreated or phosphorylated by CDPK. The reaction was initiated by the addition of [³H]L-serine, incubated for various times at 30 °C, stopped by addition of 0.2 volume of concentrated ammonium hydroxide, and incubated an additional 60 min to ensure complete conversion of OAS to N-acetylserine (NAS) (36). An aliquot of the reaction mixture was passed over 0.5 ml of AG 50W-X8 resin (Bio-Rad) to resolve NAS from serine (37). NAS was eluted with 3 ml of water, and aliquots were analyzed by liquid scintillation counting. A blank reaction containing all components except acetyl-CoA was run in parallel and subtracted as a background reading. The identities of the reaction products, the completeness of the conversion of OAS to NAS, and the efficiency of NAS elution were examined by TLC, ninhydrin staining, and autoradiography, using standard compounds as controls. TLC was performed on Cellulose 300, 100-µm TLC plates (Selecto Scientific, Inc., Suwanee, GA) and developed with butanol:water: acetic acid, 65:25:15 by volume.

Activities of full-length GmSerat2;1 constructs were determined by a spectrophotometric assay based on the absorbance of acetyl-CoA at 232 nM ( = 4500). Reactions were carried out at room temperature (23 °C) in a volume of 1 ml containing 100 mM sodium phosphate buffer, pH 7.0, 0.1 mM acetyl-CoA, 5 mM L-serine, various concentrations of L-cysteine, and 1 µg of SAT. Absorbance readings were collected every 3 s for a period of 1 min during which time the reaction rate was linear.

Specific activities determined by the two assays differed (Tables 1 and 2), but the effects of mutations, phosphorylation, and cysteine on activity were consistent, regardless of the assay used.

GmSerat2;1 COOH-terminal antibodies (cAb) were made by Cocalico Biologicals, with 20-mer peptides CPPNPIKLDKMPSFTMDHTS and CPPNPIKLDKMP(pS)FTMDHTS as antigens. Peptides were synthesized by the Interdisciplinary Center for Biotechnology Research at the University of Florida and included NH₂-terminal cysteine residues used for coupling of the peptide to SulfoLink gel (Pierce), spacer proline residues, and the COOH-terminal phosphorylation site of GmSerat2;1 (residues 368-385). A 50% ammonium sulfate fraction of the antiserum was dialyzed against antibody-binding buffer (10 mM Tris-HCl, pH 8.0, 1 mM NaF) and loaded onto the dephosphopeptide column, and then flow-through fractions were loaded onto phospho-peptide column (P-column). The P-column was washed first with 10 column volumes of antibody-binding buffer and then with 10 column volumes of washing buffer (10 mM Tris-HCl, pH 8.0, 0.5 M NaCl). Antibodies were eluted from P-column with 100 mM glycine, pH 2.0, buffer-exchanged to TBS, and concentrated. Unexpectedly, the purified antibodies recognized both dephospho- and phospho-MBP-GmSerat2; 1N equally well.

Polyclonal antibodies that specifically recognized phosphorylated GmSerat2;1 (pAb), were made by Sigma-Genosys, The Woodlands, TX, using a 13-mer peptide, CPLDKMP(pS)FT-MDH (residues 372-383 of GmSerat2;1 plus NH₂-terminal CP). Antibodies were affinity purified on a column of immobilized phosphopeptide; however, they also recognized dephosphoGmSerat2;1. To obtain the phospho-specific antibody (pAb) the latter antibodies were further purified by the same protocol used for purification of gAb, except that phospho- and dephospho-13-mer peptides were used for affinity purification. Aliquots of antibodies were stored at -20 °C.

Protein Extraction and Immunoprecipitation—Harvested soybean cells were homogenized in ice-cold extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 250 mM sucrose, 2 mM DTT, 2 mM EDTA, 2 mM EGTA, 1 mM NaF, 1 mM Na₂MoO₄, 1 mM Na₂VO_3, 1 µM Microcystin LR, 1 mM phenylmethylsulfonyl fluoride, and complete protease inhibitor mixture (Roche Diagnostics Corp.). Cell debris was pelleted by centrifugation for 60 min at 40,000 x g, and proteins were precipitated from the supernatant by ammonium sulfate at 20-50% saturation. Protein pellets were resuspended in desalting buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, 1 mM NaF, 1 mM Na₂MO₄, 1 mM Na₂VO₃). The solution was clarified by centrifugation and passed over a desalting column equilibrated with desalting buffer. The desalted fraction was supplemented with 1x complete protease inhibitor mixture (Roche Diagnostics) and 1% Nonidet P-40 and precleared of nonspecific proteins by incubating with protein-G-Sepharose (Sigma) for 2 h at 4°C. Immunoprecipitation was carried out with 5 µg of antibodies for 2 h at 4°C, with the same amount of total protein for each experiment, as determined according to Bradford using bovine serum albumin as standard. Protein-G-Sepharose was added to bring down immunocomplexes. Protein-G pellets were washed four times with desalting buffer, once with 50 mM Tris-HCl, pH 7.5, and then boiled for 5 min in 2x Laemmli SDS sample buffer. Proteins were resolved either in conventional 10% Laemmli SDS-PAGE gels using Tris-glycine buffer or in precast 10% BisTris NuPAGE gels (Invitrogen) using MOPS running buffer.

Immunoaffinity Purification of GmSerat2;1 from Cell Extracts—A 20-50% ammonium sulfate fraction of cell extract was dissolved in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, 1 mM NaF, 1 mM Na₂MO₄, and 1 mM Na₂VO₃, passed through a desalting column to exchange the buffer for Ag/Ab binding buffer (Pierce), and then loaded onto gAb-Sepharose. After the column was washed with 15 bed volumes of binding buffer, bound proteins were eluted using gentle Ag/Ab elution buffer (Pierce). Fractions with the highest absorption readings at 280 nm were pooled, dialyzed against 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, and stored in aliquots at -20 °C.

Immunoblotting Analysis—Proteins blotted onto nitrocellulose membrane were detected by incubation with the indicated primary antibody and -chain-specific goat-anti-rabbit IgG conjugated to horseradish peroxidase (Sigma) as secondary antibody. Signals were visualized with Western SuperSignal Femto substrate (Pierce) and exposure to Biomax ML film (Eastman Kodak Co.). For additional analysis of the same blot, antibodies were stripped off the membrane with Western blot Restoring Buffer (Pierce) and probed with a different primary antibody. Signals on films were scanned, digitized, and, where indicated, quantified using NIH Image (http://rsb.info.nih.gov/nih-image). Calibration was done using known concentrations of His6-CDPK. For quantification of phosphorylation and total protein of GmSerat2;1, signals were normalized to those measured for untreated cells and presented as relative units.

Protein Phosphatase Assay—Resuspended 20-50% ammonium sulfate fractions from cell extracts were desalted on a gel filtration column equilibrated in phosphatase assay buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, EDTA-free complete protease inhibitor (Roche Diagnostics)). For protein phosphatase 1 (PP1) the assay buffer was supplemented with 200 µM Mn²⁺, and the PP1-specific inhibitor PPI-2 was added for some reactions. For alkaline phosphatase, 1 mM Mg²⁺ and 0.1 mM Zn²⁺ were added to the reaction. Reactions proceeded for 10 min at 30 °C and were stopped by the addition of Microcystin LR and EDTA to final concentrations of 1 µM and 1 mM, respectively. PP1, PP2A, and PPI-2 were from Calbiochem and alkaline phosphatase from Sigma.

Subcellular Localization—Constructs encoding GmSerat2;1 fused to the NH₂ terminus of either GFP or YFP were introduced into Nicotiana benthamiana leaves or dark-germinated soybean radicles by microprojectile bombardment with a Bio-Rad PDS-1000. A Zeiss LSM 510 NLO Multiphoton confocal microscope was used to collect images 12-24 h post-bombardment.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
Identification of GmSerat2;1 by Interaction Cloning—To identify potential substrates and/or interacting proteins of soybean CDPKs, we adapted a well established interaction cloning protocol (29), which was instrumental in identification of proteins that interact with protein kinases (30, 38). Screening of 150,000 plaque-forming units for binding to three ³²P-labeled soybean CDPKs resulted in identification of eleven proteins that interacted with CDPK. To test whether these proteins were potential substrates for CDPK, phagemids were induced to express encoded proteins in E. coli, and bacterial extracts were subjected to phosphorylation assays. Interacting protein 3 (IP3) was not detectable by staining with Coomassie Blue, but it was strongly labeled by ³²P in phosphorylation assays (supplemental Fig. S1), and it was chosen for further analysis.

A data base search revealed that IP3 encoded a protein homologous to serine acetyltransferase. The full-length cDNA sequence (Fig. 1) was cloned using IP3 sequence and an EST clone (BM523465 [GenBank] ), which overlaps with the 5'-end of IP3. There is an in-frame stop codon (TAA) upstream of the putative start codon. The protein predicted from the full-length cDNA has a molecular mass of 42.5 kDa. In sequence similarity analysis the deduced protein sequence clustered with Group 2 SATs (8), which include AtSerat2;1, AtSerat2;2, and tobacco SATs 1 and 4 (supplemental Fig. S2). The gene was named GmSerat2;1 in accordance with the nomenclature proposed by Kawashima et al. (8).

View larger version (37K): [in this window] [in a new window]   FIGURE 1. Assembled nucleotide and deduced amino acid sequence of GmSerat2;1. An in-frame stop codon TAA at 5'-end-untranslated region is underlined. The solid line with arrow designates the starting nucleotide of the original clone IP3, and the dashed line with arrow denotes the first amino acid of GmSerat2;1N that was fused to MBP in the N clone. The carboxyl-terminal CDPK phosphorylation site is boxed, with the serine at position 378 being the phosphorylated residue.

GmSerat2;1 Is Localized in the Cytoplasm and with Plastids—Serine acetyltransferases are found in the cytoplasm, plastids, and mitochondria (8). To determine the subcellular localization of GmSerat2;1, full-length enzyme-tagged COOH-terminally with either green fluorescent protein (FL-GFP) or yellow fluorescent protein (FL-YFP) was transiently expressed in leaf and root cells, respectively. GFP and YFP alone were found in the cytoplasm and nucleus, but not plastids (Fig. 2, A and E). FL-GFP was found in the cytoplasm and organelles (Fig. 2B). In high resolution optical section images, these organelles were identified as chloroplasts (Fig. 2, C and D), which showed superimposed signals from chlorophyll (red autofluorescence) and FL-GFP (green). Where the GFP and chlorophyll signals overlap, the color shifted to yellow. The magnified image of the chloroplast in Fig. 2D revealed a halo of green fluorescence surrounding a core of red fluorescence. This implies that GmSerat2;1 is located on the surface or in the intermembrane space of chloroplasts. In bean radicle cells, FL-YFP was found in the cytosol and plastids (Fig. 2F).

GmSerat2.1 Is Phosphorylated on Serine 378 by CDPK—Preliminary experiments indicated that GmSerat2;1 might be a potential substrate for CDPK (supplemental Fig. S1 and data not shown). Sequence analysis of GmSerat2;1 suggested a potential phosphorylation site (Ser³⁷⁸) in the carboxyl terminus of GmSerat2;1 that matched the motif B-X-X-S/T (where B is a basic residue lysine or arginine, X is any residue, and S/T is serine or threonine) that is phosphorylated by CDPKs (39). This site was of particular interest, since bacterial SATs and plant SATs contain a regulatory site in their carboxyl termini (40-42). As shown in supplemental Fig. S3A, this motif is also present in SATs from several plant species, including chickpea, tobacco, potato, tomato, lettuce, and sunflower. Alignment of full-length protein sequences of SATs (supplemental Fig. S2), showed that the two tobacco SATs having the predicted phosphorylation site clustered with GmSerat2;1 in Group 2. The Arabidopsis SATs in Group 2, AtSerat2;1 and AtSerat2;2 (supplemental Fig. 3B) had some conserved elements of the phosphorylation motif with either Cys or Gly at the position of Ser³⁷⁸. None of the SATs in Groups 1 or 3 had any similarity to the phosphorylation motif.

To test whether the carboxyl-terminal site was the location of phosphorylation, GmSerat2;1N (encoded by clone IP3 and hereafter called N) and full-length GmSerat2;1 (hereafter called FL) were expressed in bacteria as fusion proteins with MBP at their amino termini and purified. No difference in the abilities of CDPKs , , and to phosphorylate a given protein, either N or FL, was observed (data not shown). A phosphorylation stoichiometry of up to 0.95 mol of phosphate/mol of N was observed, and this agreed with the prediction of a single CDPK phosphorylation site in this protein (data not shown). To test whether Ser³⁷⁸ was the phosphorylation site for CDPK, a mutant in which serine at this position was changed to alanine (GmSerat2;1N/S378A, hereafter called N/S378A) was also expressed and purified. During a 40-min incubation with His₆-CDPK and [-³²P]ATP, N was phosphorylated to a stoichiometry of 0.7 mol of phosphate/mol of protein, while negligible phosphorylation was detected for N/S378A (Fig. 3A). No phosphorylation was detected for MBP alone (data not shown). To ask whether additional CDPK phosphorylation sites were present in full-length GmSerat2;1, equal amounts of N, FL, and two phosphorylation site mutants, FL/S378A and FL/S378D (full-length GmSerat2;1 in which Ser³⁷⁸ was replaced by Asp), were phosphorylated by CDPK (Fig. 3B). In comparison to FL (lane 2), phosphorylation of the two phosphorylation site mutants was negligible (lanes 3 and 4), showing that Ser³⁷⁸ is the only CDPK phosphorylation site in these proteins. FL was not phosphorylated as well as N(lanes 2 and 5). Similar results were obtained when these proteins were phosphorylated by CDPK (data not shown).

View larger version (71K): [in this window] [in a new window]   FIGURE 2. Localization of GmSerat2;1 in the cytoplasm and plastids. Constructs encoding GmSerat2;1 fused to the N terminus of either GFP or YFP were transiently expressed in the indicated tissues and visualized 12-24 h post-bombardment. A-D, N. benthamiana leaves. E and F, bean radicles. C, a higher resolution optical section of B with dual GFP (green) and chlorophyll (red) channels. D, a magnified image of highlighted area in C. Note that the autofluorescent chlorophyll on the top left of C and D are from a neighboring guard cell and that the orange colors of organelles resulted from the overlap of green and red fluorescence. Images are representative of >30 cells expressing individual plasmids and three independent bombardment experiments of each construct. Bars, 10 µm.

View larger version (35K): [in this window] [in a new window]   FIGURE 3. Phosphorylation and activity of GmSerat2:1 and mutant proteins. A, either Nor N/S378A was incubated on ice with His6-CDPK plus [-32P]ATP in Ca2+-containing assay buffer for the indicated times (minutes). Samples taken at the indicated time points were resolved on 10% SDS-PAGE (upper panel) and analyzed by autoradiography (lower panel). Bands corresponding to GmSerat2;1 proteins were cut from the gel, and 32P incorporation was determined by scintillation counting, and phosphorylation stoichiometry (mole %) was calculated. Additional His6-CDPK protein was added to the reactions 30 min following the initiation of the phosphorylation. B, 1 µg of protein (lane 1, MBP; lane 2, MBP-FL; lane 3, MBP-FL/S378A; lane 4, MBP-FL/S378D; lane 5, MBP-N) was incubated for 30 min with 0.1 µg of CDPK in phosphorylation buffer with [-32P]ATP. Proteins were resolved on an SDS-PAGE gel (left panel), and the gel was autoradiographed (right panel). C-F, effect of cysteine on the activity of unphosphorylated and phosphorylated GmSerat2;1 and mutants. One microgram of the indicated enzymes was incubated for 30 min with or without 0.1 µg of CDPK in phosphorylation buffer with ATP. Following the phosphorylation reaction proteins were tested by the spectrophotometric assay for SAT activity in the presence of various concentrations of cysteine. Data are the averages and standard deviations for assays performed in triplicate. Dose-response curves for the effect of cysteine on the indicated enzymes incubated without and with CDPK.

Mutation of Ser³⁷⁸ to either Ala or Asp did not significantly affect specific activity in the absence of cysteine (Table 1 and Fig. 4), showing that these mutations did not affect the ability of the enzyme to catalyze the formation of OAS. However, these mutations had dramatically different effects on the sensitivity to inhibition by cysteine. Mutation of Ser³⁷⁸ to Ala lowered the IC₅₀ for cysteine inhibition to 18 µM, whereas mutation to Asp rendered the enzyme insensitive to cysteine inhibition (Fig. 3, E and F). Replacement of a phosphorylatable serine residue with aspartate introduces a negative charge that can mimic the effect of phosphorylation, and these data show that the S378D mutant mimics fully phosphorylated GmSerat2;1. Incubation of these mutants in the phosphorylation reaction, which results in negligible phosphorylation of either protein (Fig. 3A), had no effect on the IC₅₀ for Cys inhibition for either mutant (Fig. 3, E and F). These data further support the conclusion that phosphorylation of Ser³⁷⁸ by CDPK blocks feedback inhibition by cysteine.

View larger version (25K): [in this window] [in a new window]   FIGURE 4. Phosphorylation desensitizes MBP-GmSerat2;1 N(N) from feedback inhibition by cysteine. Either MBP-N(N) (A) or MBP-N/S378A (N/S378A) (B) was preincubated on ice with His6-CDPK and ATP in Ca2+-containing buffer for the indicated period of time (-, no preincubation; 0, preincubation in Ca2+-free buffer), and then samples were analyzed for phosphorylation stoichiometry as well as catalytic activity in the absence or presence of 1 mML-cysteine. SAT activity was measured in the radiometric assay. Error bars represent standard errors of duplicate reactions. The maximal activity (100%) was 73.7 µmol of NAS/mg of protein/min, measured in the absence of cysteine. C, correlation between activity and phosphorylation state. The activities of N prephosphorylated to various degrees were determined in the presence of cysteine and plotted as a function of the phosphorylation stoichiometry of the enzyme. An enzyme activity of 100% is equivalent to the activity of N assayed in the absence of cysteine. The line drawn on the graph was determined by linear regression.

View larger version (65K): [in this window] [in a new window]   FIGURE 5. GmSerat2;1 is phosphorylated in vivo. A-C, characterization of GmSerat2;1 antibodies. MBP-GmSerat2;1/N fusion proteins, either unphosphorylated (N) or phosphorylated by CDPK in vitro (Np), were analyzed by Western blot with general antibodies gAb (A), untreated phosphorylation state-specific antibodies pAb (B), or pAb pretreated with either GmSerat2;1 phosphopeptide or GmSerat2;1 dephosphopeptide (C). D, proteins purified from soybean cell extract using an affinity column conjugated with gAb (see "Experimental Procedures") were analyzed by Western blot with different antibodies: C, control with secondary antibody alone; gAb, general GmSerat2;1 antibody; cAb, COOH-terminal GmSerat2;1 antibody; pAb, untreated phosphospecific antibody; pAb P; phospho-specific antibody pretreated with GmSerat2;1 phosphopeptide CPLDKMP(pS)FTMDH; pAb D, phospho-specific antibody pretreated with GmSerat2;1 dephosphopeptide CPLDKMPSFTMDH. The asterisk designates a nonspecific protein that is immunostained by the secondary antibody.

GmSerat2;1 Is Phosphorylated in Vivo—To study the phosphorylation of GmSerat2;1 in vivo, antibodies that specifically recognize the phosphorylated enzyme or that recognize both phospho- and dephospho-GmSerat2;1 were made. gAb, cAb, and pAb were produced using GmSerat1;2-N, C-terminal peptide, and phosphorylated C-terminal-peptide, respectively. Western blot analysis showed gAb recognized both dephosphorylated and phosphorylated N (Fig. 5A), while pAb recognized only N phosphorylated by CDPK in vitro (Fig. 5B). Preabsorption of pAb with the 13-mer phosphopeptide corresponding to the C terminus of GmSerat2;1, but not with the 13 mer dephosphopeptide, completely blocked the recognition of phosphorylated N by pAb (Fig. 5C), indicating pAb is specific for the phosphorylation site containing phospho-Ser³⁷⁸.

Western blot analysis with these antibodies was performed to test whether GmSerat2;1 is phosphorylated in vivo (Fig. 5D). Due to the low abundance of GmSerat2:1 in vivo (data not shown), it was necessary to enrich the enzyme by immunoaffinity chromatography with Sepharose-conjugated gAb prior to analysis. All three GmSerat2;1 antibodies, gAb, cAb, and pAb, recognized a protein of 42 kDa, the size predicted for endogenous GmSerat2;1. Preincubation of pAb with the 13-mer phosphopeptide of GmSerat2;1 (pAb/P), but not with the 13-mer dephosphopeptide (pAb/D), abolished the recognition of GmSerat2;1 by pAb, indicating that it is phosphorylated in vivo at the predicted phosphorylation site, Ser³⁷⁸. Besides the 42-kDa GmSerat2;1 band, gAb recognized an additional 29-kDa protein. This protein may be the soybean SAT isoform reported by Chronis and Krishnan (53).

PP1 Dephosphorylates GmSerat2;1—To gain insight into which phosphatase is responsible for the dephosphorylation of GmSerat2;1, cell extracts were treated with three different protein phosphatases in vitro. Immunoprecipitation (IP) was carried out with gAb, and then IP pellets were analyzed by Western blot with pAb as well as gAb. As seen in supplemental Fig. S4C, PP1, but not PP2A or alkaline phosphatase, dephosphorylated GmSerat2;1. PP1 was unable to dephosphorylate GmSerat2;1 when PPI-2, a specific peptide inhibitor to PP1, was included in the reaction, indicating phosphatase activity of PP1 was specific to dephosphorylation of GmSerat2;1.

Hydrogen Peroxide Induces the Synthesis and Phosphorylation of GmSerat2;1 in Vivo—Exogenous application of H₂O₂ is known to trigger a rise in [Ca²⁺]_cyt (21, 24) and thus potentially activates CDPKs (35). To ask whether changes in the abundance and phosphorylation state of GmSerat2;1 occur in response to oxidative stress in vivo, we used antibodies to analyze levels of phosphorylated and total GmSerat2;1 following exposure of soybean cells to exogenous H₂O₂. As seen in Fig. 6, the levels of both total and phosphorylated GmSerat2;1 protein increased following H₂O₂ treatment. After 60 min of H₂O₂ treatment, the level of total GmSerat2;1 was 3-fold higher than that in control cells, and it remained elevated for at least 4 h after oxidant challenge. Analysis of the ratio of phosphorylated to total GmSerat2;1 protein showed that between 5 and 30 min the fraction of phosphorylated protein increased relative to the total. On average, a 2-5-fold increase in the ratio was evident as soon as 5 min following H₂O₂ treatment. After 30 min the fraction of phosphorylated enzyme declined as the total protein level increased, but the fraction of phosphorylated protein present at 240 min post-H₂O₂ treatment was nearly double that present at the beginning of the experiment.

View larger version (37K): [in this window] [in a new window]   FIGURE 6. Time course of GmSerat2;1 in response to H2O2 treatment. A, cultured soybean cells were collected at the indicated times following treatment with 0.5 mM H2O2. gAb immunoprecipitates were analyzed by Western blot with pAb and gAb to determine the levels of phosphorylated (phospho) and total (total) GmSerat2;1, respectively. These results are representative of two experiments. B, quantitative analysis of data shown in A. The plot shows the total protein level relative to that of untreated cells (circles) and ratio of phosphorylated to total GmSerat2;1 protein normalized to the ratio for untreated cells (squares).

View larger version (32K): [in this window] [in a new window]   FIGURE 7. Effect of inhibitors of protein synthesis and phosphorylation and Ca2+ antagonists on H2O2-induced phosphorylation of GmSerat2;1. A, external Ca2+ is not required for GmSerat2;1 phosphorylation or synthesis. Soybean cells were treated with 2 mM EGTA for 1 h prior to a 2-h treatment with 0.5 mM H2O2. B, internal Ca2+ is required for H2O2-induced GmSerat2;1 synthesis. Soybean cells were pretreated for 15 min with internal Ca2+ inhibitors, 200 µM A9C or 50 µM NA, or dimethyl sulfoxide (DMSO) (solvent), prior to a 1-h treatment with 0.5 mM H2O2. C, soybean cells were treated with 30 mM cycloheximide (CHX) for 1 h prior to the application of 0.5 mM H2O2. Cells were harvested after an additional 30 or 60 min. D, decoupling of H2O2-induced synthesis of GmSerat2;1 from its phosphorylation. Soybean cells were pretreated with 2 µM ST (a general protein kinase inhibitor) or dimethyl sulfoxide (DMSO) (solvent of ST) for 15 min prior to a 1-h treatment with 0.5 mM H2O2. In all experiments proteins were immunoprecipitated with gAb and analyzed by Western blot with pAb and gAb to determine the levels of phosphorylated (phospho) and total (total) proteins, respectively.

The Accumulation of GmSerat2;1 in Response to Hydrogen Peroxide Treatment Results from Increased Protein Synthesis—To assess whether the increased GmSerat2;1 protein level, observed 1 h after H₂O₂ treatment, was due to an increase in protein synthesis or a decrease in protein degradation, we treated cells with cycloheximide, a protein synthesis inhibitor, prior to H₂O₂ treatment. Fig. 7C shows that pretreatment of cells with cycloheximide blocked the H₂O₂-induced increase in levels of total GmSerat2;1 protein (compare the total protein levels in the two 60-min samples), indicating that exposure to H₂O₂ induced synthesis of GmSerat2;1 rather than suppressing its degradation in soybean cells.

Phosphorylation Does Not Affect the Stability of GmSerat2;1—A change in the phosphorylation state of a protein can lead to its proteosome-mediated protein destruction (45, 46). To ask whether inhibition of phosphorylation would increase GmSerat2;1 stability, and to also ask whether phosphorylation events are involved in the signaling pathway leading to H₂O₂-induced GmSerat2;1 synthesis, cells were treated with staurosporine (ST), a general protein kinase inhibitor, prior to oxidant challenge and levels of phosphorylated and total GmSerat2;1 were determined. As seen in Fig. 7D, while ST pretreatment almost completely abolished phosphorylation, it had little effect on the increased accumulation of total GmSerat2;1 protein, which was equivalent to that in cells without ST pretreatment. This observation suggests that phosphorylation events are not required for H₂O₂-induced GmSerat2;1 synthesis. Consistent with the idea that phosphorylation of GmSerat2;1 is not associated with its degradation, levels of both relative phosphorylation and total protein increased in cells following H₂O₂ treatment (Fig. 6). Most importantly, in experiments in which protein synthesis was inhibited by cycloheximide, no decrease in the level of GmSerat2;1 was observed upon H₂O₂-induced increase in phosphorylation (compare lane 4 with lane 6 in Fig. 7C).

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
In a screen for proteins that interact with CDPKs, we identified the serine acetyltransferase GmSerat2;1. We demonstrated that GmSerat2;1 is a substrate for CDPK in vitro (Figs. 3 and 4) and that it is phosphorylated in vivo in response to oxidative stress (Fig. 6). While phosphorylation has no direct effect on the activity of the enzyme, it blocks inhibition of activity by cysteine (Figs. 3 and 4), the end product of the biosynthetic pathway in which serine acetyltransferase participates. Our observations that protein kinase activity present in soybean cell extracts phosphorylates GmSerat2;1 (supplemental Fig. S4) and that the phosphorylation is greatly stimulated by Ca²⁺ (Fig. 7) are consistent with the idea that GmSerat2;1 activity is regulated by CDPK in vivo.

GFP-tagged GmSerat2;1 localized in the cytoplasm and with plastids (Fig. 2). This result must be interpreted with caution because of possible mislocalization due to overexpression of the GFP-tagged enzyme under the control of the 35S promoter. However, the result is consistent with the dual localization Arabidopsis SAT-p (AtSerat2;1) (9). This SAT was localized with plastids in 4-week-old leaves but found predominantly in the cytoplasm in 6-week-old leaves. Localization of this SAT to plastids was questioned by Droux (2), since in vitro translated protein (then called SAT5) was not imported into chloroplasts (47). Localization of the enzyme to the outside of the chloroplasts could explain both sets of observations. Interestingly, SAT-p is the only one of the five Arabidopsis SATs that is associated with plastids (8, 9). CDPKs are found in the cytoplasm, plasma membrane, endoplasmic reticulum, and peroxisomes (48). Cytoplasmic CDPKs would have access to SAT located in the cytoplasm or on the surface of plastids.

The IC₅₀ for cysteine inhibition of GmSerat2;1 is 40 µM, which places it in the class of SATs that have intermediate sensitivity to cysteine. Other SATs in this class are Allium tubero-sum ASAT5 (IC₅₀ 49 µM) (49) and SAT1 (IC₅₀ 40 µM) and SAT7 (IC₅₀ 30 µM) from Nicotiana tabacum (3). SATs that are highly sensitive to cysteine are Arabidopsis thaliana AtSerat1;1 (IC₅₀ 1.8 µM) (9) and AtSerat3;2 (0.8 µM) (8), Citrullus vulgaris SAT (2.9 µM) (50), Allium cepa SAT (3.1 µM) (51), Spinacia oleracea SAT (7.6 µM) (52), Pisum sativum SAT (12 µM) (2), and G. max SSAT1 (a Group 1 SAT; 4 µM) (53).

The cellular concentration of cysteine is thought to be 2-10 µM (54). The IC₅₀ values of the intermediate sensitivity class of SATs (30-50 µM) are high in comparison to this value, and this difference casts some doubt on the importance of the intermediate sensitivity class in regulating cysteine in vivo; however, the variation in cysteine concentration in the cytosol, mitochondria, plastids, and vacuole is not known, so the actual concentration of cysteine experienced by SATs in each compartment is not known. Experiments comparing the effect of tobacco SATs on the production of cysteine in bacteria suggest the potential of various sensitivity classes of SATs to limit cysteine synthesis in planta. When the endogenous SAT (CysE) of E. coli (IC₅₀ 1 µM) was replaced by tobacco SAT1 (IC₅₀ 40 µM) or SAT7 (IC₅₀ 30 µM) cysteine accumulation increased 3-4-fold, whereas replacement by SAT4, which is not inhibited by cysteine, supported a 50-fold increase in cysteine (55). These results suggest that intermediately sensitive forms of SAT support only slightly higher production of cysteine than do highly sensitive SATs and that they limit cysteine synthesis relative to SATs that are not inhibited by cysteine. Our data show that phosphorylation of GmSerat1;2 converts it from an intermediately sensitive to an insensitive SAT, which would increase the accumulation of cysteine.

Analysis of plant SAT sequences available in electronic data bases (supplemental Fig. S3) shows that, in addition to soybean, species from several families of angiosperms and gymnosperms have SATs in which the COOH-terminal phosphorylation site is conserved. Interestingly, SATs from Arabidopsis and rice lack this site, thus phosphorylation of SAT is not universal. It has been noted that there is species variation in cysteine synthesis and sulfur metabolism (10), and now the regulation by phosphorylation of SATs from a subset of plants adds to the complexity. The observed variation shows the importance of studying the regulation of cysteine synthesis in plants on a species-to-species and organelle-to-organelle basis.

The phosphorylation site in GmSerat2;1 identified in this study, serine 378, is located close to the site found to be critical for feedback inhibition of SATs from watermelon (41, 42) and Arabidopsis (56). Substitution of a single amino acid residue, either Gly²⁷⁷ to Cys or Met²⁸⁰ to Ile, in the C-terminal region of watermelon SAT causes a significant decrease in its sensitivity to cysteine inhibition (41, 42). The comparable residues in GmSerat2;1 are Ser³⁷⁸ and Met³⁸¹ (supplemental Fig. S3B). The change of serine 378 to alanine or aspartic acid in GmSerat2;1 did not affect its catalytic activity, but mutation to aspartic acid mimicked phosphorylation and rendered the enzyme insensitive to feedback regulation by cysteine. In the crystal structure of cysteine-inhibited SAT from Haemophilus influenzae (43), residues of the COOH terminus contact cysteine, which is bound at the active site. Introduction of negative charge into the COOH terminus of GmSerat2;1 by either phosphorylation or mutation of Ser³⁷⁸ to Asp probably prevents the COOH terminus from interacting with Cys and stabilizing of its binding to the active site.

Residues in the COOH terminus of SATs from plants and bacteria are important for not only feedback inhibition by cysteine but also for the binding of SAT to OAS-TL (3, 6, 43, 57, 58). The observation that cysteine causes the complex between Cys-inhibited SAT and OAS-TL to dissociate (2) suggests a model in which the COOH terminus can be involved in binding to either OAS-TL or to cysteine but not both. The dual function of the SAT C terminus is supported by crystal structures of bacterial SAT (43), described above, and OAS-TL. In the structure of E. coli OAS-TL crystallized with the COOH-terminal peptide from H. influenzae SAT, the SAT peptide is located in the active site of OAS-TL (58), and this interaction provides the basis for inhibition of OAS-TL in the cysteine synthase complex (2).

Addition of H₂O₂ to soybean cells stimulated phosphorylation and synthesis of GmSerat2;1 within 5 min of treatment (Fig. 6). The timing of this response is consistent with the observation that treatment of tobacco cells and Arabidopsis seedlings with H₂O₂ resulted in a transient increase in cytoplasmic calcium within 1 min of treatment (21, 24). Experiments with pharmacological effectors A9C and niflumic acid (Fig. 7) suggested that internally derived calcium was required for both rapidly induced GmSerat2;1 phosphorylation and increased protein synthesis in the late phase of the response to oxidant challenge. The sites of action of these agents in plant cells are not well defined, so these results should be viewed as tentative. Under normal culturing conditions, GmSerat2;1 exhibited a basal level of phosphorylation, which was greatly diminished by treatment with EGTA (supplemental Fig. S5 and Fig. 7).

Based on data presented in this paper we propose a model for GmSerat2;1 regulation (Fig. 8). Signals such as abiotic or biotic stress increase intracellular free calcium (21-26, 44, 59), which in turn activates CDPK to phosphorylate and increases synthesis of GmSerat2;1. This dual response upon oxidant challenge provides a mechanism by which cells can respond quickly to H₂O₂ exposure through desensitized GmSerat2;1 to avoid the depletion of the cysteine pool and then signal for more GmSerat2;1 protein to be synthesized to relieve a sustained stress condition. CDPK is able to couple Ca²⁺ signaling to the defense response by regulating a particular target.

View larger version (40K): [in this window] [in a new window]   FIGURE 8. A proposed working model. GmSerat2;1 protein exists in two states: a dephosphorylated state, which is sensitive to feedback inhibition by cysteine, and an active phosphorylated state. Oxidative stress induces a rapid phosphorylation of GmSerat2;1 to prevent potential depletion of cysteine pool and an increase in total protein to tolerate the sustained stress through the potential increase of GSH. Externally derived Ca2+ contributes to retaining GmSerat2;1 at a basal level of phosphorylation, while Ca2+ released from internal storage sites such as endoplasmic reticulum, vacuoles, mitochondria, and chloroplasts is required for the activation of CDPK and other Ca2+-modulated proteins such as calmodulins, which may regulate the transcription or translation of GmSerat2;1 in response to oxidative stress.

	FOOTNOTES

 
The nucleotide sequence(s) reported in this paper has been submitted to the Gen-BankTM/EBI Data Bank with accession number(s) AY422685 [GenBank] .

^* This work was supported in part by National Science Foundation Grant MCB-9604647 (to A. C. H.) and by funds from the University of Florida (to A. C. H.) and from the University of Delaware and the College of Agriculture and Natural Resources Research Partnership (to B.-C. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement"in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1-S5.

This article was selected as a Paper of the Week.

¹ Current address: Dept. of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA 02115.

² Current address: Crop Genetics Research, E.I. du Pont de Nemours and Company, Wilmington, DE 19880-0353.

³ Supported by National Institutes of Health Grant P20 RR-15588 from the National Center for Research Resources.

⁴ To whom correspondence should be addressed: Cancer/Genetics Complex, University of Florida, P. O. Box 103610, Gainesville, FL 32610-3610. Tel.: 352-273-8096; Fax: 352-392-3993; E-mail: harmon{at}ufl.edu.

⁵ The abbreviations used are: SAT, serine acetyltransferase; OAS, O-acetylserine, OAS-TL, O-acetylserine (thiol)lyase; IP3, clone encoding interacting protein 3; GmSerat2;1, G. max serine acetyltransferase 2;1; CDPK, calcium-dependent protein kinase; MBP, maltose-binding protein; PP, protein phosphatase; IP3, interacting protein 3; EST, expressed sequence tag; GFP, green fluorescent protein; YFP, yellow fluorescent protein; FL, full-length G. max serine acetyltransferase 2;1; N, amino-terminal deleted version of GmSerat2;1 encoded by IP3; gAb, general antibody; cAb, C-terminal antibody; pAb, phospho-specific antibody; IP, immunoprecipitation; A9C, anthracene-9-carboxylate; ST, staurosporine; NAS, N-acetylserine; DTT, dithiothreitol; BisTris, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1, 3-diol; MOPS, 4-morpholinepropanesulfonic acid.

⁶ J.-Y. Lee, unpublished data.

	ACKNOWLEDGMENTS

 
We appreciate the gift of a soybean cell cDNA expression library from R. Tenhaken and C. Lamb. We thank B. Rathinasabapathi for advice and help during the development of the radiometric assay for SAT activity.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

 
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