HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 14, 10581-10584, April 6, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/14/10581    most recent C000806200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, S. Articles by Park, D. Search for Related Content PubMed PubMed Citation Articles by Kim, S. Articles by Park, D. Social Bookmarking                      What's this?

ACCELERATED PUBLICATION
Leucine Zipper-mediated Homodimerization of the p21-activated Kinase-interacting Factor, Pix
IMPLICATION FOR A ROLE IN CYTOSKELETAL REORGANIZATION^*

Seyun Kim, Seung-Hye Lee, and Dongeun Park

From the School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea

Pix, a p21-activated kinase-interacting exchange factor, is known to be involved in the regulation of Cdc42/Rac GTPases. The 85-kDa Pix-a protein contains an Src homology 3 domain, the tandem Dbl homology and Pleckstrin homology domains, a proline-rich region, and a GIT1-binding domain. In addition to those domains, Pix-a also contains a putative leucine zipper domain at the C-terminal end. In this study, we demonstrate that the previously identified putative leucine zipper domain mediates the formation of Pix-a homodimers. Using in vitro and in vivo methodologies, we show that deletion of the leucine zipper domain is sufficient to abolish Pix-a homodimerization. In NIH3T3 fibroblast cells, expression of wild type Pix-a induces the formation of membrane ruffles. However, cells expressing the leucine zipper domain deletion mutant could not form membrane ruffle structures. Moreover, platelet-derived growth factor-mediated cytoskeletal changes were completely blocked by the leucine zipper domain deletion mutant. The results suggest that the leucine zipper domain enables Pix-a to homodimerize, and homodimerization is essential for Pix-a signaling functions leading to the cytoskeletal reorganization.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Z. M. Zhang, J. A. Simmerman, C. D. Guibao, and J. J. Zheng GIT1 Paxillin-binding Domain Is a Four-helix Bundle, and It Binds to Both Paxillin LD2 and LD4 Motifs J. Biol. Chem., July 4, 2008; 283(27): 18685 - 18693. [Abstract] [Full Text] [PDF]

				C. Mionnet, S. Bogliolo, and R. A. Arkowitz Oligomerization Regulates the Localization of Cdc24, the Cdc42 Activator in Saccharomyces cerevisiae J. Biol. Chem., June 20, 2008; 283(25): 17515 - 17530. [Abstract] [Full Text] [PDF]

				A. Chahdi and A. Sorokin Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt Mol. Biol. Cell, June 1, 2008; 19(6): 2609 - 2619. [Abstract] [Full Text] [PDF]

				A. Chahdi and A. Sorokin Protein Kinase A-Dependent Phosphorylation Modulates {beta}1Pix Guanine Nucleotide Exchange Factor Activity through 14-3-3{beta} Binding Mol. Cell. Biol., March 1, 2008; 28(5): 1679 - 1687. [Abstract] [Full Text] [PDF]

				M. W. Mayhew, E. D. Jeffery, N. E. Sherman, K. Nelson, J. M. Polefrone, S. J. Pratt, J. Shabanowitz, J. T. Parsons, J. W. Fox, D. F. Hunt, et al. Identification of phosphorylation sites in betaPIX and PAK1 J. Cell Sci., November 15, 2007; 120(22): 3911 - 3918. [Full Text] [PDF]

				E.-Y. Shin, C.-S. Lee, T. G. Cho, Y. G. Kim, S. Song, Y.-S. Juhnn, S. C. Park, E. Manser, and E.-G. Kim betaPak-interacting Exchange Factor-mediated Rac1 Activation Requires smgGDS Guanine Nucleotide Exchange Factor in Basic Fibroblast Growth Factor-induced Neurite Outgrowth J. Biol. Chem., November 24, 2006; 281(47): 35954 - 35964. [Abstract] [Full Text] [PDF]

				L. Za, C. Albertinazzi, S. Paris, M. Gagliani, C. Tacchetti, and I. de Curtis {beta}PIX controls cell motility and neurite extension by regulating the distribution of GIT1 J. Cell Sci., July 1, 2006; 119(13): 2654 - 2666. [Abstract] [Full Text] [PDF]

				R. J. Hoefen and B. C. Berk The multifunctional GIT family of proteins. J. Cell Sci., April 15, 2006; 119(Pt 8): 1469 - 1475. [Abstract] [Full Text] [PDF]

				L. Baisamy, N. Jurisch, and D. Diviani Leucine Zipper-mediated Homo-oligomerization Regulates the Rho-GEF Activity of AKAP-Lbc J. Biol. Chem., April 15, 2005; 280(15): 15405 - 15412. [Abstract] [Full Text] [PDF]

				R. Kunita, A. Otomo, H. Mizumura, K. Suzuki, J. Showguchi-Miyata, Y. Yanagisawa, S. Hadano, and J.-E Ikeda Homo-oligomerization of ALS2 through Its Unique Carboxyl-terminal Regions Is Essential for the ALS2-associated Rab5 Guanine Nucleotide Exchange Activity and Its Regulatory Function on Endosome Trafficking J. Biol. Chem., September 10, 2004; 279(37): 38626 - 38635. [Abstract] [Full Text] [PDF]

				N. Meller, M. Irani-Tehrani, B. I. Ratnikov, B. M. Paschal, and M. A. Schwartz The Novel Cdc42 Guanine Nucleotide Exchange Factor, Zizimin1, Dimerizes via the Cdc42-binding CZH2 Domain J. Biol. Chem., September 3, 2004; 279(36): 37470 - 37476. [Abstract] [Full Text] [PDF]

				H. S. Park, S. H. Lee, D. Park, J. S. Lee, S. H. Ryu, W. J. Lee, S. G. Rhee, and Y. S. Bae Sequential Activation of Phosphatidylinositol 3-Kinase, {beta}Pix, Rac1, and Nox1 in Growth Factor-Induced Production of H2O2 Mol. Cell. Biol., May 15, 2004; 24(10): 4384 - 4394. [Abstract] [Full Text] [PDF]

				S. Edlund, M. Landstrom, C.-H. Heldin, and P. Aspenstrom Smad7 is required for TGF-{beta}-induced activation of the small GTPase Cdc42 J. Cell Sci., May 1, 2004; 117(9): 1835 - 1847. [Abstract] [Full Text] [PDF]

				E.-Y. Shin, K.-N. Woo, C.-S. Lee, S.-H. Koo, Y. G. Kim, W.-J. Kim, C.-D. Bae, S.-I. Chang, and E.-G. Kim Basic Fibroblast Growth Factor Stimulates Activation of Rac1 through a p85 {beta}PIX Phosphorylation-dependent Pathway J. Biol. Chem., January 16, 2004; 279(3): 1994 - 2004. [Abstract] [Full Text] [PDF]

				Y. J. Im, J. H. Lee, S. H. Park, S. J. Park, S.-H. Rho, G. B. Kang, E. Kim, and S. H. Eom Crystal Structure of the Shank PDZ-Ligand Complex Reveals a Class I PDZ Interaction and a Novel PDZ-PDZ Dimerization J. Biol. Chem., November 28, 2003; 278(48): 48099 - 48104. [Abstract] [Full Text] [PDF]

				T. M. Eisenhaure, S. A. Francis, L. D. Willison, S. R. Coughlin, and D. J. Lerner The Rho Guanine Nucleotide Exchange Factor Lsc Homo-oligomerizes and Is Negatively Regulated through Domains in Its Carboxyl Terminus That Are Absent in Novel Splenic Isoforms J. Biol. Chem., August 15, 2003; 278(33): 30975 - 30984. [Abstract] [Full Text] [PDF]

				E. Park, M. Na, J. Choi, S. Kim, J.-R. Lee, J. Yoon, D. Park, M. Sheng, and E. Kim The Shank Family of Postsynaptic Density Proteins Interacts with and Promotes Synaptic Accumulation of the {beta}PIX Guanine Nucleotide Exchange Factor for Rac1 and Cdc42 J. Biol. Chem., May 23, 2003; 278(21): 19220 - 19229. [Abstract] [Full Text] [PDF]

				S. Kim, J. Ko, H. Shin, J.-R. Lee, C. Lim, J.-H. Han, W. D. Altrock, C. C. Garner, E. D. Gundelfinger, R. T. Premont, et al. The GIT Family of Proteins Forms Multimers and Associates with the Presynaptic Cytomatrix Protein Piccolo J. Biol. Chem., February 14, 2003; 278(8): 6291 - 6300. [Abstract] [Full Text] [PDF]

				E.-Y. Shin, K.-S. Shin, C.-S. Lee, K.-N. Woo, S.-H. Quan, N.-K. Soung, Y. G. Kim, C. I. Cha, S.-R. Kim, D. Park, et al. Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific Guanine Nucleotide Exchange Factor, via the Ras/ERK/PAK2 Pathway Is Required for Basic Fibroblast Growth Factor-induced Neurite Outgrowth J. Biol. Chem., November 8, 2002; 277(46): 44417 - 44430. [Abstract] [Full Text] [PDF]

				M. C. Brown, K. A. West, and C. E. Turner Paxillin-dependent Paxillin Kinase Linker and p21-Activated Kinase Localization to Focal Adhesions Involves a Multistep Activation Pathway Mol. Biol. Cell, May 1, 2002; 13(5): 1550 - 1565. [Abstract] [Full Text] [PDF]

				C.-G. Koh, E. Manser, Z.-S. Zhao, C.-P. Ng, and L. Lim {beta}1PIX, the PAK-interacting exchange factor, requires localization via a coiled-coil region to promote microvillus-like structures and membrane ruffles J. Cell Sci., January 12, 2001; 114(23): 4239 - 4251. [Abstract] [Full Text] [PDF]