JBC Biosymposia, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M103378200 on May 21, 2001

J. Biol. Chem., Vol. 276, Issue 30, 27936-27943, July 27, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/30/27936    most recent
M103378200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hofmann, R. M.
Right arrow Articles by Pickart, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hofmann, R. M.
Right arrow Articles by Pickart, C. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

In Vitro Assembly and Recognition of Lys-63 Polyubiquitin Chains*

Roseanne M. HofmannDagger and Cecile M. Pickart§

From the Department of Biochemistry and Molecular Biology, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205

Polyubiquitin chains assembled through lysine 48 (Lys-48) of ubiquitin act as a signal for substrate proteolysis by 26 S proteasomes, whereas chains assembled through Lys-63 play a mechanistically undefined role in post-replicative DNA repair. We showed previously that the products of the UBC13 and MMS2 genes function in error-free post-replicative DNA repair in the yeast Saccharomyces cerevisiae and form a complex that assembles Lys-63-linked polyubiquitin chains in vitro. Here we confirm that the Mms2·Ubc13 complex functions as a high affinity heterodimer in the chain assembly reaction in vitro and report the results of a kinetic characterization of the polyubiquitin chain assembly reaction. To test whether a Lys-63-linked polyubiquitin chain can signal degradation, we conjugated Lys-63-linked tetra-ubiquitin to a model substrate of 26 S proteasomes. Although the noncanonical chain effectively signaled substrate degradation, the results of new genetic epistasis studies agree with previous genetic data in suggesting that the proteolytic activity of proteasomes is not required for error-free post-replicative repair.

* This work was supported by National Institutes of Health (NIH) Grant GM60372 and by a training grant from the NIEHS, NIH.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Rm. 8041, Johns Hopkins University, 615 N. Wolfe St. Baltimore, MD 21205. Tel.: 410-614-4554; Fax: 410-955-2926; E-mail: cpickart@welchlink.welch.jhu.edu.

Dagger Present address: Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, NY 10021.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
R. Wen, J. A. Torres-Acosta, L. Pastushok, X. Lai, L. Pelzer, H. Wang, and W. Xiao
Arabidopsis UEV1D Promotes Lysine-63-Linked Polyubiquitination and Is Involved in DNA Damage Response
PLANT CELL, January 1, 2008; 20(1): 213 - 227.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Barriere, C. Nemes, K. Du, and G. L. Lukacs
Plasticity of Polyubiquitin Recognition as Lysosomal Targeting Signals by the Endosomal Sorting Machinery
Mol. Biol. Cell, October 1, 2007; 18(10): 3952 - 3965.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. T. Kim, K. P. Kim, F. Lledias, A. F. Kisselev, K. M. Scaglione, D. Skowyra, S. P. Gygi, and A. L. Goldberg
Certain Pairs of Ubiquitin-conjugating Enzymes (E2s) and Ubiquitin-Protein Ligases (E3s) Synthesize Nondegradable Forked Ubiquitin Chains Containing All Possible Isopeptide Linkages
J. Biol. Chem., June 15, 2007; 282(24): 17375 - 17386.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
F. P. Marx, A. S. Soehn, D. Berg, C. Melle, C. Schiesling, M. Lang, S. Kautzmann, K. M. Strauss, T. Franck, S. Engelender, et al.
The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease
FASEB J, June 1, 2007; 21(8): 1759 - 1767.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
K. Dreher and J. Callis
Ubiquitin, Hormones and Biotic Stress in Plants
Ann. Bot., May 1, 2007; 99(5): 787 - 822.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Fukushima, S.-i. Matsuzawa, C. L. Kress, J. M. Bruey, M. Krajewska, S. Lefebvre, J. M. Zapata, Z. Ronai, and J. C. Reed
Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses
PNAS, April 10, 2007; 104(15): 6371 - 6376.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
S. Tuvia, D. Taglicht, O. Erez, I. Alroy, I. Alchanati, V. Bicoviski, M. Dori-Bachash, D. Ben-Avraham, and Y. Reiss
The ubiquitin E3 ligase POSH regulates calcium homeostasis through spatial control of Herp
J. Cell Biol., April 9, 2007; 177(1): 51 - 61.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
D. Kunze, D. MacCallum, F. C. Odds, and B. Hube
Multiple functions of DOA1 in Candida albicans
Microbiology, April 1, 2007; 153(4): 1026 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. T. Lis and F. E. Romesberg
Role of Doa1 in the Saccharomyces cerevisiae DNA Damage Response.
Mol. Cell. Biol., June 1, 2006; 26(11): 4122 - 4133.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
I. Kim and H. Rao
What's Ub Chain Linkage Got to Do with It?
Sci. Signal., April 11, 2006; 2006(330): pe18 - pe18.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Okumura, G. Lu, I. Pitha-Rowe, and P. M. Pitha
Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15
PNAS, January 31, 2006; 103(5): 1440 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Shang, G. Deng, Q. Liu, W. Guo, A. L. Haas, B. Crosas, D. Finley, and A. Taylor
Lys6-modified Ubiquitin Inhibits Ubiquitin-dependent Protein Degradation
J. Biol. Chem., May 27, 2005; 280(21): 20365 - 20374.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Tsui, A. Raguraj, and C. M. Pickart
Ubiquitin Binding Site of the Ubiquitin E2 Variant (UEV) Protein Mms2 Is Required for DNA Damage Tolerance in the Yeast RAD6 Pathway
J. Biol. Chem., May 20, 2005; 280(20): 19829 - 19835.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. W. Doss-Pepe, L. Chen, and K. Madura
{alpha}-Synuclein and Parkin Contribute to the Assembly of Ubiquitin Lysine 63-linked Multiubiquitin Chains
J. Biol. Chem., April 29, 2005; 280(17): 16619 - 16624.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
A. Laine and Z. Ronai
Ubiquitin Chains in the Ladder of MAPK Signaling
Sci. Signal., April 26, 2005; 2005(281): re5 - re5.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Huang, Q. Huang, X. Zhou, M. M. Shen, A. Yen, S. X. Yu, G. Dong, K. Qu, P. Huang, E. M. Anderson, et al.
The Poxvirus p28 Virulence Factor Is an E3 Ubiquitin Ligase
J. Biol. Chem., December 24, 2004; 279(52): 54110 - 54116.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Tenno, K. Fujiwara, H. Tochio, K. Iwai, E. H. Morita, H. Hayashi, S. Murata, H. Hiroaki, M. Sato, K. Tanaka, et al.
Structural basis for distinct roles of Lys63- and Lys48-linked polyubiquitin chains
Genes Cells, October 1, 2004; 9(10): 865 - 875.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. L. Seibenhener, J. R. Babu, T. Geetha, H. C. Wong, N. R. Krishna, and M. W. Wooten
Sequestosome 1/p62 Is a Polyubiquitin Chain Binding Protein Involved in Ubiquitin Proteasome Degradation
Mol. Cell. Biol., September 15, 2004; 24(18): 8055 - 8068.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. Matsusaka and J. Pines
Chfr acts with the p38 stress kinases to block entry to mitosis in mammalian cells
J. Cell Biol., August 16, 2004; 166(4): 507 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. L. Ramsey, J. J. Smith, A. Dasgupta, N. Maqani, P. Grant, and D. T. Auble
The NEF4 Complex Regulates Rad4 Levels and Utilizes Snf2/Swi2-Related ATPase Activity for Nucleotide Excision Repair
Mol. Cell. Biol., July 15, 2004; 24(14): 6362 - 6378.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Kim, K. Mi, and H. Rao
Multiple Interactions of Rad23 Suggest a Mechanism for Ubiquitylated Substrate Delivery Important in Proteolysis
Mol. Biol. Cell, July 1, 2004; 15(7): 3357 - 3365.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Varadan, M. Assfalg, A. Haririnia, S. Raasi, C. Pickart, and D. Fushman
Solution Conformation of Lys63-linked Di-ubiquitin Chain Provides Clues to Functional Diversity of Polyubiquitin Signaling
J. Biol. Chem., February 20, 2004; 279(8): 7055 - 7063.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Didier, L. Broday, A. Bhoumik, S. Israeli, S. Takahashi, K. Nakayama, S. M. Thomas, C. E. Turner, S. Henderson, H. Sabe, et al.
RNF5, a RING Finger Protein That Regulates Cell Motility by Targeting Paxillin Ubiquitination and Altered Localization
Mol. Cell. Biol., August 1, 2003; 23(15): 5331 - 5345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Raasi and C. M. Pickart
Rad23 Ubiquitin-associated Domains (UBA) Inhibit 26 S Proteasome-catalyzed Proteolysis by Sequestering Lysine 48-linked Polyubiquitin Chains
J. Biol. Chem., March 7, 2003; 278(11): 8951 - 8959.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. D. Ulrich
Protein-Protein Interactions within an E2-RING Finger Complex. IMPLICATIONS FOR UBIQUITIN-DEPENDENT DNA DAMAGE REPAIR
J. Biol. Chem., February 21, 2003; 278(9): 7051 - 7058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Alberti, J. Demand, C. Esser, N. Emmerich, H. Schild, and J. Hohfeld
Ubiquitylation of BAG-1 Suggests a Novel Regulatory Mechanism during the Sorting of Chaperone Substrates to the Proteasome
J. Biol. Chem., November 22, 2002; 277(48): 45920 - 45927.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kiakos, T. T. Howard, M. Lee, J. A. Hartley, and P. J. McHugh
Saccharomyces cerevisiae RAD5 Influences the Excision Repair of DNA Minor Groove Adducts
J. Biol. Chem., November 8, 2002; 277(46): 44576 - 44581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Rao and A. Sastry
Recognition of Specific Ubiquitin Conjugates Is Important for the Proteolytic Functions of the Ubiquitin-associated Domain Proteins Dsk2 and Rad23
J. Biol. Chem., March 29, 2002; 277(14): 11691 - 11695.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
H. D. Ulrich
Degradation or Maintenance: Actions of the Ubiquitin System on Eukaryotic Chromatin
Eukaryot. Cell, February 1, 2002; 1(1): 1 - 10.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.