JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106438200 on July 25, 2001

J. Biol. Chem., Vol. 276, Issue 42, 38949-38955, October 19, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/42/38949    most recent
M106438200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dewilde, S.
Right arrow Articles by Moens, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dewilde, S.
Right arrow Articles by Moens, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Biochemical Characterization and Ligand Binding Properties of Neuroglobin, a Novel Member of the Globin Family*

Sylvia DewildeDagger §, Laurent Kiger§||, Thorsten Burmester**, Thomas HankelnDagger Dagger , Veronique Baudin-Creuza||, Tony AertsDagger , Michael C. Marden||, Roland Caubergs§§, and Luc MoensDagger ¶¶

From the Dagger  Department of Biochemistry, University of Antwerp, B-2610 Antwerp, Belgium, || INSERM, Unite 473, Hôpital de Bicètre, F94275 Le Kremlin-Bicètre, France, the ** Institute of Zoology, Johannes Gutenberg University of Mainz, D-55099 Mainz, Germany, the Dagger Dagger  Institute of Molecular Genetics, Biosafety Research and Consulting, Johannes Gutenberg University of Mainz, D-55099 Mainz, Germany, and the §§ Department of Biology, University of Antwerp, B-2610 Antwerp, Belgium

Neuroglobin is a recently discovered member of the globin superfamily that is suggested to enhance the O2 supply of the vertebrate brain. Spectral measurements with human and mouse recombinant neuroglobin provide evidence for a hexacoordinated deoxy ferrous (Fe2+) form, indicating a His-Fe2+-His binding scheme. O2 or CO can displace the endogenous protein ligand, which is identified as the distal histidine by mutagenesis. The ferric (Fe3+) form of neuroglobin is also hexacoordinated with the protein ligand E7-His and does not exhibit pH dependence. Flash photolysis studies show a high recombination rate (kon) and a slow dissociation rate (koff) for both O2 and CO, indicating a high intrinsic affinity for these ligands. However, because the rate-limiting step in ligand combination with the deoxy hexacoordinated form involves the dissociation of the protein ligand, O2 and CO binding is suggested to be slow in vivo. Because of this competition, the observed O2 affinity of recombinant human neuroglobin is average (1 torr at 37 °C). Neuroglobin has a high autoxidation rate, resulting in an oxidation at 37 °C by air within a few minutes. The oxidation/reduction potential of mouse neuroglobin (E'o = -129 mV) lies within the physiological range. Under natural conditions, recombinant mouse neuroglobin occurs as a monomer with disulfide-dependent formation of dimers. The biochemical and kinetic characteristics are discussed in view of the possible functions of neuroglobin in the vertebrate brain.

* This work was supported in part by funds from INSERM, Association Recherche et Transfusion Contract 21-2000, by a collaboration of University and INSERM, and by Deutsche Forschungsgemeinschaft Grant Ha3201/3;Bu956/3.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

Post-doctoral fellow of the Fund for Scientific Research (Flanders).

¶¶ Supported by Grant G.0069.98 of the Fund for Scientific Research (Flanders). To whom correspondence should be addressed: Dept. of Biochemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Tel.: 32-3-820-2323; Fax: 32-3-820-2248; E-mail: lmoens@uia.ua.ac.be.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
M. Anselmi, M. Brunori, B. Vallone, and A. Di Nola
Molecular Dynamics Simulation of the Neuroglobin Crystal: Comparison with the Simulation in Solution
Biophys. J., November 1, 2008; 95(9): 4157 - 4162.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
S. Dewilde, A. I. Ioanitescu, L. Kiger, K. Gilany, M. C. Marden, S. Van Doorslaer, J. Vercruysse, A. Pesce, M. Nardini, M. Bolognesi, et al.
The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: A molecular biological, physico-chemical, kinetic, and vaccination study
Protein Sci., October 1, 2008; 17(10): 1653 - 1662.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. T. Gladwin and D. B. Kim-Shapiro
The functional nitrite reductase activity of the heme-globins
Blood, October 1, 2008; 112(7): 2636 - 2647.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. Ostojic, S. Grozdanic, N. A. Syed, M. S. Hargrove, J. T. Trent III, M. H. Kuehn, R. H. Kardon, Y. H. Kwon, and D. S. Sakaguchi
Neuroglobin and Cytoglobin Distribution in the Anterior Eye Segment: A Comparative Immunohistochemical Study
J. Histochem. Cytochem., September 1, 2008; 56(9): 863 - 872.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. F. Alzawahra, M. A. H. Talukder, X. Liu, A. Samouilov, and J. L. Zweier
Heme proteins mediate the conversion of nitrite to nitric oxide in the vascular wall
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H499 - H508.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Yamashita, L. Bouzhir-Sima, J.-C. Lambry, U. Liebl, and M. H. Vos
Ligand Dynamics and Early Signaling Events in the Heme Domain of the Sensor Protein Dos from Escherichia coli
J. Biol. Chem., January 25, 2008; 283(4): 2344 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
M. T. Wilson and B. J. Reeder
Oxygen-binding haem proteins
Exp Physiol, January 1, 2008; 93(1): 128 - 132.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Thijs, E. Vinck, A. Bolli, F. Trandafir, X. Wan, D. Hoogewijs, M. Coletta, A. Fago, R. E. Weber, S. Van Doorslaer, et al.
Characterization of a Globin-coupled Oxygen Sensor with a Gene-regulating Function
J. Biol. Chem., December 28, 2007; 282(52): 37325 - 37340.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Ishikawa, I. J. Finkelstein, S. Kim, K. Kwak, J. K. Chung, K. Wakasugi, A. M. Massari, and M. D. Fayer
Neuroglobin dynamics observed with ultrafast 2D-IR vibrational echo spectroscopy
PNAS, October 9, 2007; 104(41): 16116 - 16121.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Anselmi, M. Brunori, B. Vallone, and A. Di Nola
Molecular Dynamics Simulation of Deoxy and Carboxy Murine Neuroglobin in Water
Biophys. J., July 15, 2007; 93(2): 434 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Hamdane, C. Vasseur-Godbillon, V. Baudin-Creuza, G. H. B. Hoa, and M. C. Marden
Reversible Hexacoordination of {alpha}-Hemoglobin-stabilizing Protein (AHSP)/{alpha}-Hemoglobin Versus Pressure: EVIDENCE FOR PROTECTION OF THE {alpha}-CHAINS BY THEIR CHAPERONE
J. Biol. Chem., March 2, 2007; 282(9): 6398 - 6404.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
J. A. Hoy, B. J. Smagghe, P. Halder, and M. S. Hargrove
Covalent heme attachment in Synechocystis hemoglobin is required to prevent ferrous heme dissociation
Protein Sci., February 1, 2007; 16(2): 250 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. A. Khan, Y. Wang, Y. Sun, X. O. Mao, L. Xie, E. Miles, J. Graboski, S. Chen, L. M. Ellerby, K. Jin, et al.
Neuroglobin-overexpressing transgenic mice are resistant to cerebral and myocardial ischemia
PNAS, November 21, 2006; 103(47): 17944 - 17948.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Dewilde, B. Ebner, E. Vinck, K. Gilany, T. Hankeln, T. Burmester, J. Kreiling, C. Reinisch, J. R. Vanfleteren, L. Kiger, et al.
The Nerve Hemoglobin of the Bivalve Mollusc Spisula solidissima: MOLECULAR CLONING, LIGAND BINDING STUDIES, AND PHYLOGENETIC ANALYSIS
J. Biol. Chem., March 3, 2006; 281(9): 5364 - 5372.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. Ostojic, D. S. Sakaguchi, Y. de Lathouder, M. S. Hargrove, J. T. Trent III, Y. H. Kwon, R. H. Kardon, M. H. Kuehn, D. M. Betts, and S. Grozdanic
Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1016 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
C. Vasseur-Godbillon, D. Hamdane, M. C. Marden, and V. Baudin-Creuza
High-yield expression in Escherichia coli of soluble human {alpha}-hemoglobin complexed with its molecular chaperone
Protein Eng. Des. Sel., March 1, 2006; 19(3): 91 - 97.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Hamdane, L. Kiger, G. H. B. Hoa, S. Dewilde, J. Uzan, T. Burmester, T. Hankeln, L. Moens, and M. C. Marden
High Pressure Enhances Hexacoordination in Neuroglobin and Other Globins
J. Biol. Chem., November 4, 2005; 280(44): 36809 - 36814.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. I. Ioanitescu, S. Dewilde, L. Kiger, M. C. Marden, L. Moens, and S. Van Doorslaer
Characterization of Nonsymbiotic Tomato Hemoglobin
Biophys. J., October 1, 2005; 89(4): 2628 - 2639.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Brunori, A. Giuffre, K. Nienhaus, G. U. Nienhaus, F. M. Scandurra, and B. Vallone
Neuroglobin, nitric oxide, and oxygen: Functional pathways and conformational changes
PNAS, June 14, 2005; 102(24): 8483 - 8488.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bentmann, M. Schmidt, S. Reuss, U. Wolfrum, T. Hankeln, and T. Burmester
Divergent Distribution in Vascular and Avascular Mammalian Retinae Links Neuroglobin to Cellular Respiration
J. Biol. Chem., May 27, 2005; 280(21): 20660 - 20665.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Vallone, K. Nienhaus, A. Matthes, M. Brunori, and G. U. Nienhaus
The structure of carbonmonoxy neuroglobin reveals a heme-sliding mechanism for control of ligand affinity
PNAS, December 14, 2004; 101(50): 17351 - 17356.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Fago, C. Hundahl, S. Dewilde, K. Gilany, L. Moens, and R. E. Weber
Allosteric Regulation and Temperature Dependence of Oxygen Binding in Human Neuroglobin and Cytoglobin: MOLECULAR MECHANISMS AND PHYSIOLOGICAL SIGNIFICANCE
J. Biol. Chem., October 22, 2004; 279(43): 44417 - 44426.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Uzan, S. Dewilde, T. Burmester, T. Hankeln, L. Moens, D. Hamdane, M. C. Marden, and L. Kiger
Neuroglobin and Other Hexacoordinated Hemoglobins Show a Weak Temperature Dependence of Oxygen Binding
Biophys. J., August 1, 2004; 87(2): 1196 - 1204.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Fuchs, V. Heib, L. Kiger, M. Haberkamp, A. Roesner, M. Schmidt, D. Hamdane, M. C. Marden, T. Hankeln, and T. Burmester
Zebrafish Reveals Different and Conserved Features of Vertebrate Neuroglobin Gene Structure, Expression Pattern, and Ligand Binding
J. Biol. Chem., June 4, 2004; 279(23): 24116 - 24122.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
T. Burmester and T. Hankeln
Neuroglobin: A Respiratory Protein of the Nervous System
Physiology, June 1, 2004; 19(3): 110 - 113.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Herold, A. Fago, R. E. Weber, S. Dewilde, and L. Moens
Reactivity Studies of the Fe(III) and Fe(II)NO Forms of Human Neuroglobin Reveal a Potential Role against Oxidative Stress
J. Biol. Chem., May 28, 2004; 279(22): 22841 - 22847.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Nienhaus, J. M. Kriegl, and G. U. Nienhaus
Structural Dynamics in the Active Site of Murine Neuroglobin and Its Effects on Ligand Binding
J. Biol. Chem., May 28, 2004; 279(22): 22944 - 22952.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Uno, D. Ryu, H. Tsutsumi, Y. Tomisugi, Y. Ishikawa, A. J. Wilkinson, H. Sato, and T. Hayashi
Residues in the Distal Heme Pocket of Neuroglobin: IMPLICATIONS FOR THE MULTIPLE LIGAND BINDING STEPS
J. Biol. Chem., February 13, 2004; 279(7): 5886 - 5893.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Taguchi, T. Matsui, J. Igarashi, Y. Sasakura, Y. Araki, O. Ito, S. Sugiyama, I. Sagami, and T. Shimizu
Binding of Oxygen and Carbon Monoxide to a Heme-regulated Phosphodiesterase from Escherichia coli: KINETICS AND INFRARED SPECTRA OF THE FULL-LENGTH WILD-TYPE ENZYME, ISOLATED PAS DOMAIN, AND MET-95 MUTANTS
J. Biol. Chem., January 30, 2004; 279(5): 3340 - 3347.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Hamdane, L. Kiger, S. Dewilde, B. N. Green, A. Pesce, J. Uzan, T. Burmester, T. Hankeln, M. Bolognesi, L. Moens, et al.
The Redox State of the Cell Regulates the Ligand Binding Affinity of Human Neuroglobin and Cytoglobin
J. Biol. Chem., December 19, 2003; 278(51): 51713 - 51721.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Wakasugi, T. Nakano, and I. Morishima
Oxidized Human Neuroglobin Acts as a Heterotrimeric G{alpha} Protein Guanine Nucleotide Dissociation Inhibitor
J. Biol. Chem., September 19, 2003; 278(38): 36505 - 36512.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Geuens, I. Brouns, D. Flamez, S. Dewilde, J.-P. Timmermans, and L. Moens
A Globin in the Nucleus!
J. Biol. Chem., August 15, 2003; 278(33): 30417 - 30420.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Kundu, S. A. Premer, J. A. Hoy, J. T. Trent III, and M. S. Hargrove
Direct Measurement of Equilibrium Constants for High-Affinity Hemoglobins
Biophys. J., June 1, 2003; 84(6): 3931 - 3940.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Sun, K. Jin, A. Peel, X. O. Mao, L. Xie, and D. A. Greenberg
Neuroglobin protects the brain from experimental stroke invivo
PNAS, March 18, 2003; 100(6): 3497 - 3500.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Van Doorslaer, S. Dewilde, L. Kiger, S. V. Nistor, E. Goovaerts, M. C. Marden, and L. Moens
Nitric Oxide Binding Properties of Neuroglobin. A CHARACTERIZATION BY EPR AND FLASH PHOTOLYSIS
J. Biol. Chem., February 7, 2003; 278(7): 4919 - 4925.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Schmidt, A. Giessl, T. Laufs, T. Hankeln, U. Wolfrum, and T. Burmester
How Does the Eye Breathe? EVIDENCE FOR NEUROGLOBIN-MEDIATED OXYGEN SUPPLY IN THE MAMMALIAN RETINA
J. Biol. Chem., January 10, 2003; 278(3): 1932 - 1935.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
P. P.A. Mammen, J. M. Shelton, S. C. Goetsch, S. C. Williams, J. A. Richardson, M. G. Garry, and D. J. Garry
Neuroglobin, A Novel Member of the Globin Family, Is Expressed in Focal Regions of the Brain
J. Histochem. Cytochem., December 1, 2002; 50(12): 1591 - 1598.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Hankeln, V. Jaenicke, L. Kiger, S. Dewilde, G. Ungerechts, M. Schmidt, J. Urban, M. C. Marden, L. Moens, and T. Burmester
Characterization of Drosophila Hemoglobin. EVIDENCE FOR HEMOGLOBIN-MEDIATED RESPIRATION IN INSECTS
J. Biol. Chem., August 2, 2002; 277(32): 29012 - 29017.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Kriegl, A. J. Bhattacharyya, K. Nienhaus, P. Deng, O. Minkow, and G. U. Nienhaus
Ligand binding and protein dynamics in neuroglobin
PNAS, June 11, 2002; 99(12): 7992 - 7997.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Sun, K. Jin, X. O. Mao, Y. Zhu, and D. A. Greenberg
Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury
PNAS, December 6, 2001; (2001) 251466698.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Sun, K. Jin, X. O. Mao, Y. Zhu, and D. A. Greenberg
Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury
PNAS, December 18, 2001; 98(26): 15306 - 15311.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.