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Papers In Press, published online ahead of print January 10, 2001
Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205
Corresponding Author: vyang{at}jhmi.edu
Intestinal-enriched Krüppel like factor (IKLF or KLF5) belongs to the family of mammalian Krüppel-like transcription factors. Previous studies indicate that expression of IKLF is enriched in the proliferating crypt epithelial cells of the intestinal tract. However, the biological function of IKLF is unknown. In the current study, we showed that the levels of IKLF mRNA were nearly undetectable in serum-deprived NIH3T3 fibroblasts but became acutely and significantly increased upon the addition of fetal bovine serum or the phorbol ester, PMA. This induction required protein synthesis as it was prevented by cycloheximide. Transfection of IKLF into NIH3T3 cells resulted in the formation of foci in a manner similar to that caused by the Ha-Ras oncogene. Constitutive expression of IKLF in transfected NIH3T3 cells significantly increased the rate of proliferation when compared to cells transfected with an empty vector. The growth of IKLF-transfected cells was no longer inhibited by cell-cell contact or by low serum content. Moreover, these cells proliferated in an anchorage-independent fashion. We conclude that IKLF encodes a delayed early response gene product that positively regulates cellular proliferation and may give rise to a transformed phenotype when over-expressed.
J. Biol. Chem, 10.1074/jbc.C000870200
Submitted on December 11, 2000
Revised on January 10, 2001
Accepted on January 10, 2001
Intestinal-enriched Krüppel like factor (Krüppel-like factor 5) is a positive regulator of cellular proliferation
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