JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 6, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/37/33541    most recent
C200309200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hill, W. G.
Right arrow Articles by Johnson, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hill, W. G.
Right arrow Articles by Johnson, J. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print August 6, 2002
J. Biol. Chem, 10.1074/jbc.C200309200
Submitted on May 19, 2002
Revised on July 31, 2002
Accepted on August 6, 2002

Endogenously expressed Epithelial sodium channel is present in lipid rafts in A6 cells

Warren G. Hill, Bing An, and John P. Johnson

Renal Division/Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261

Corresponding Author: whill+{at}pitt.edu

The epithelial sodium channel (ENaC) present in kidney collecting duct, distal colon and the lung is responsible for salt reabsorption and whole body volume regulation. It is composed of three homologous subunits, alpha , beta and gamma and mutations to these subunits can lead to the salt wasting disease pseudohypoaldosteronism type I, associated with decreased channel density at the plasma membrane; or to the hypertensive disorder, Liddle's syndrome, in which channel residency time at the plasma membrane is enhanced. Regulation of ENaC trafficking and turnover is therefore critical to sodium homeostasis. In this study we examined whether ENaC is present in the cholesterol-enriched microdomains commonly called lipid rafts, in the endogenously expressing A6 cell line. We demonstrate that a fraction of alpha , beta and gamma -ENaC are present in detergent-insoluble membranes, that subunits exist in membranes which float on discontinuous sucrose density gradients and that methyl-beta -cyclodextrin treatment causes a redistribution of ENaC subunits to higher density membranes. Furthermore, chronic aldosterone stimulation results in a shift in the membrane density of all three subunits. Biotinylation of apical membrane proteins revealed that ENaC is present in lipid rafts on the plasma membrane. In conclusion, these results show that ENaC is present in lipid rafts both intracellularly and on the cell surface. Raft association may be important for trafficking and/or function of the channel.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W. G. Hill, M. B. Butterworth, H. Wang, R. S. Edinger, J. Lebowitz, K. W. Peters, R. A. Frizzell, and J. P. Johnson
The Epithelial Sodium Channel (ENaC) Traffics to Apical Membrane in Lipid Rafts in Mouse Cortical Collecting Duct Cells
J. Biol. Chem., December 28, 2007; 282(52): 37402 - 37411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. T. T. Nguyen, L. Charrier-Hisamuddin, G. Dalmasso, A. Hiol, S. Sitaraman, and D. Merlin
Association of PepT1 with lipid rafts differently modulates its transport activity in polarized and nonpolarized cells
Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1155 - G1165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mies, C. Spriet, L. Heliot, and S. Sariban-Sohraby
Epithelial Na+ Channel Stimulation by n-3 Fatty Acids Requires Proximity to a Membrane-bound A-kinase-anchoring Protein Complexed with Protein Kinase A and Phosphodiesterase
J. Biol. Chem., June 22, 2007; 282(25): 18339 - 18347.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. Welker, B. Geist, J.-H. Fruhauf, M. Salanova, D. A. Groneberg, E. Krause, and S. Bachmann
Role of lipid rafts in membrane delivery of renal epithelial Na+-K+-ATPase, thick ascending limb
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1328 - R1337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. M. Verghese, M. F. Gutknecht, and G. H. Caughey
Prostasin regulates epithelial monolayer function: cell-specific Gpld1-mediated secretion and functional role for GPI anchor
Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1258 - C1270.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Murtazina, O. Kovbasnjuk, M. Donowitz, and X. Li
Na+/H+ Exchanger NHE3 Activity and Trafficking Are Lipid Raft-dependent
J. Biol. Chem., June 30, 2006; 281(26): 17845 - 17855.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Balut, P. Steels, M. Radu, M. Ameloot, W. V. Driessche, and D. Jans
Membrane cholesterol extraction decreases Na+ transport in A6 renal epithelia
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C87 - C94.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H.-P. Ma and D. C. Eaton
Acute Regulation of Epithelial Sodium Channel by Anionic Phospholipids
J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3182 - 3187.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Bachhuber, J. Konig, T. Voelcker, B. Murle, R. Schreiber, and K. Kunzelmann
Cl- Interference with the Epithelial Na+ Channel ENaC
J. Biol. Chem., September 9, 2005; 280(36): 31587 - 31594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. Malik, Q. Yue, G. Yue, X. J. Chen, S. R. Price, W. E. Mitch, and D. C. Eaton
Role of Nedd4-2 and polyubiquitination in epithelial sodium channel degradation in untransfected renal A6 cells expressing endogenous ENaC subunits
Am J Physiol Renal Physiol, July 1, 2005; 289(1): F107 - F116.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Caldwell, R. C. Boucher, and M. J. Stutts
Neutrophil elastase activates near-silent epithelial Na+ channels and increases airway epithelial Na+ transport
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L813 - L819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Mies, V. Shlyonsky, A. Goolaerts, and S. Sariban-Sohraby
Modulation of epithelial Na+ channel activity by long-chain n-3 fatty acids
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F850 - F855.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Mohan, J. R. Bruns, K. M. Weixel, R. S. Edinger, J. B. Bruns, T. R. Kleyman, J. P. Johnson, and O. A. Weisz
Differential Current Decay Profiles of Epithelial Sodium Channel Subunit Combinations in Polarized Renal Epithelial Cells
J. Biol. Chem., July 30, 2004; 279(31): 32071 - 32078.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
M. S. Awayda, W. Shao, F. Guo, M. Zeidel, and W. G. Hill
ENaC-Membrane Interactions: Regulation of Channel Activity by Membrane Order
J. Gen. Physiol., June 1, 2004; 123(6): 709 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Berdiev, B. Jovov, W. C. Tucker, A. P. Naren, C. M. Fuller, E. R. Chapman, and D. J. Benos
ENaC subunit-subunit interactions and inhibition by syntaxin 1A
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1100 - F1106.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
W. G. Hill, M. A. Kaetzel, B. K. Kishore, J. R. Dedman, and M. L. Zeidel
Annexin A4 Reduces Water and Proton Permeability of Model Membranes but Does Not Alter Aquaporin 2-mediated Water Transport in Isolated Endosomes
J. Gen. Physiol., April 28, 2003; 121(5): 413 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. W. Leung, R. G. Contreras, C. Flores-Maldonado, M. Cereijido, and E. Rodriguez-Boulan
Inhibitors of glycosphingolipid biosynthesis reduce transepithelial electrical resistance in MDCK I and FRT cells
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C1021 - C1030.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.