| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print April 3, 2000
Department of Biology, University of California, San Diego, La Jolla, CA 92093-0322
Corresponding Author: dhelinski{at}ucsd.edu
Replication initiation of the broad host range plasmid RK2 requires binding of the host-encoded DnaA protein to specific sequences (DnaA boxes) at its replication origin (oriV). In contrast to a chromosomal replication origin, which functionally interacts only with the native DnaA protein of the organism, the ability of RK2 to replicate in a wide range of Gram-negative bacterial hosts requires the interaction of oriV with many different DnaA proteins. In this study we compared the interactions of oriV with five different DnaA proteins. DNAse I footprint, gel mobility shift and surface plasmon resonance analyses showed that the DnaA proteins from Escherichia coli, Pseudomonas putida and Pseudomonas aeruginosa bind to the DnaA boxes at oriV and are capable of inducing open complex formation, the first step in the replication initiation process. However, DnaA proteins from two Gram-positive bacteria, Bacillus subtilis and Streptomyces lividans, while capable of specifically interacting with the DnaA box sequences at oriV, do not bind stably, and fail to induce open complex formation. These results suggest that the inability of the DnaA protein of a host bacterium to form a stable and functional complex with the DnaA boxes at oriV is a limiting step for plasmid host range.
J. Biol. Chem, 10.1074/jbc.M000552200
Submitted on January 24, 2000
Revised on March 28, 2000
Accepted on April 2, 2000
Interactions of DnaA Proteins From Distantly Related Bacteria with the Replication Origin of the Broad Host Range Plasmid RK2
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Rajewska, L. Kowalczyk, G. Konopa, and I. Konieczny Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading PNAS, August 12, 2008; 105(32): 11134 - 11139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Jarvis, A. A. Beaudry, J. M. Bullard, N. Janjic, and C. S. McHenry Reconstitution of a Minimal DNA Replicase from Pseudomonas aeruginosa and Stimulation by Non-cognate Auxiliary Factors J. Biol. Chem., March 4, 2005; 280(9): 7890 - 7900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Betteridge, J. Yang, A. J. Pittard, and J. Praszkier Role of RepA and DnaA Proteins in the Opening of the Origin of DNA Replication of an IncB Plasmid J. Bacteriol., June 15, 2004; 186(12): 3785 - 3793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhong, D. Helinski, and A. Toukdarian A Specific Region in the N Terminus of a Replication Initiation Protein of Plasmid RK2 Is Required for Recruitment of Pseudomonas aeruginosa DnaB Helicase to the Plasmid Origin J. Biol. Chem., November 14, 2003; 278(46): 45305 - 45310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang, M. Pacek, D. R. Helinski, I. Konieczny, and A. Toukdarian A multifunctional plasmid-encoded replication initiation protein both recruits and positions an active helicase at the replication origin PNAS, July 22, 2003; 100(15): 8692 - 8697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Konieczny and K. Liberek Cooperative Action of Escherichia coli ClpB Protein and DnaK Chaperone in the Activation of a Replication Initiation Protein J. Biol. Chem., May 17, 2002; 277(21): 18483 - 18488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pacek, G. Konopa, and I. Konieczny DnaA Box Sequences as the Site for Helicase Delivery during Plasmid RK2 Replication Initiation in Escherichia coli J. Biol. Chem., June 22, 2001; 276(26): 23639 - 23644. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
