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Papers In Press, published online ahead of print July 18, 2000
Biological Science, Graduate School of Science, Nagoya University, Nagoya-shi, Aichi-ken 464-8602
Corresponding Author: g44416a{at}nucc.cc.nagoya-u.ac.jp
PomA and PomB form a complex that conducts sodium ions and generates the torque for the Na+-driven polar flagellar motor of Vibrio alginolyticus. PomA has four transmembrane segments, and one periplasmic loop (loop1-2) connects segments 1 and 2 and another (loop3-4), in which cysteine-scanning mutagenesis had been carried out, connects segments 3 and 4. When PomA with an introduced Cys residue (Cys-PomA) in the C-terminal periplasmic loop (loop3-4) was examined without exposure to a reducing reagent, a 43-kDa band was observed, whereas only a 25-kDa band, which corresponds to monomeric PomA, was observed under reducing conditions. The intensity of the 43-kDa band was enhanced in most mutants by the oxidizing reagent, CuCl2. The 43-kDa band was strongest in the P172C mutant. The motility of the P172C mutant was severely reduced and P172C showed a dominant-negative effect, whereas substitution of Pro with Ala, Ile or Ser at this position did not affect motility. In the presence of DTT, the swimming speed was restored by reducing the amount of 43-kDa protein. These results suggest that the disulfide cross-link disturbs the function of PomA. When the mutated Cys residue was modified with NEM, only the 25-kDa band was detected and the 43-kDa form seemed to consist of only the 25-kDa protein. These results suggest that the 43-kDa band is an intermolecular cross-linked product of PomA and the loops3-4 of adjacent subunits of PomA are probably close to each other in the motor complex. We propose that this loop region is important for dimer formation and motor function.
J. Biol. Chem, 10.1074/jbc.M000848200
Submitted on February 3, 2000
Revised on July 17, 2000
Accepted on July 17, 2000
Intermolecular cross-linking between the periplasmic loop3-4 regions of PomA, a component of the Na+-driven flagellar motor of Vibrio alginolyticus
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