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Papers In Press, published online ahead of print September 5, 2000
Department of Life Sciences (Chemistry), The University of Tokyo, Tokyo 153-8902
Corresponding Author: csyanag{at}mail.ecc.u-tokyo.ac.jp
Sp1 is one of the well-documented transcription factors, but the whole structure of human Sp1 has not been determined yet. In the present study, we isolated several cDNAs representing two forms of human Sp1 mRNA with different 5? terminal structures in HepG2 cells. Isolation of a genomic clone established that one of the cDNAs represents the mRNA having consecutive alignment of exons, which allowed to deduce the complete amino acid sequence for human Sp1. Another cDNA clone had a surprising structure, which possessed an alignment of exons 3-2-3. Both RT-PCR and RNase protection assays confirmed accumulation of two forms of Sp1 mRNA in HepG2 cells. Since Southern blot analysis suggested that exon 3 is of a single copy in the genome, the cDNA clone having the duplicated sequences for exon 3 appeared to reflect an unusual RNA editing mechanism, i.e., trans-splicing between identical pre-mRNAs of human Sp1.
J. Biol. Chem, 10.1074/jbc.M002010200
Submitted on March 9, 2000
Accepted on September 5, 2000
Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing
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