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Papers In Press, published online ahead of print July 5, 2000
Departamento de Bioquímica Medica, Centro Ciencias da Saude, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590
Corresponding Author: pmourao{at}hucff.ufrj.br
We have characterized the structure of a sulfated D-galactan from the red algae Botryocladia occidentalis. The following repeating structure [-4-alpha-D-Galp-1-3-beta-D-Galp-1-]was found for this polysaccharide, but with a variable sulfation pattern. Clearly approximately one third of the total alpha-units are 2,3-di-O-sulfated and another one third are 2-O-sulfated. The algal sulfated D-galactan has a potent anticoagulant activity (similar potency as unfractionated heparin) due to enhanced inhibition of thrombin and factor Xa by antithrombin and/or heparin cofactor II. We also extended the experiments to several sulfated polysaccharides from marine invertebrates with simple structures, composed of a single repeating structure. A 2-O- or 3-O-sulfated L-galactan (as well as a 2-O-sulfated L-fucan) has a weak anticoagulant action when compared with the potent action of the algal sulfated D-galactan. Possibly, the addition of two sulfate esters to a single alpha-galactose residue has an ?amplifying effect? on the anticoagulant action, which can not be totally ascribed to the increased charge density of the polymer. These results indicate that the wide diversity of polysaccharides from marine alga and invertebrates is an useful tool to elucidate structure/anticoagulant activity relationships.
J. Biol. Chem, 10.1074/jbc.M002422200
Submitted on March 22, 2000
Accepted on July 5, 2000
Structure and anticoagulant activity of sulfated galactans. Isolation of a Unique sulfated galactan from the red algae botryocladia occidentalis and comparison of its anticoagulant action with that of a sulfated galactans from Invertebrates
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