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Papers In Press, published online ahead of print July 10, 2000
Department of Biochemistry, University of Stellenbosch, Stellenbosch 7602
Corresponding Author: jr{at}maties.sun.ac.za
The kinetic parameters in vitro of the components of the phosphoenolpyruvate:glucose phosphotransferase system (PTS) in enteric bacteria were collected. To address the issue whether the behavior in vivo of the PTS can be understood in terms of these enzyme kinetics, a detailed kinetic model was constructed. Each overall phosphotransfer reaction was separated into two elementary reactions, the first entailing association of the phosphoryl donor and acceptor into a complex, and the second entailing dissociation of the complex into dephosphorylated donor and phosphorylated acceptor. Literature data on the Km values and association constants of PTS proteins for their substrates, as well as equilibrium and rate constants for the overall phosphotransfer reactions, were related to the rate constants of the elementary steps in a set of equations; the rate constants could be calculated by solving these equations simultaneously. No kinetic parameters were fitted. As calculated by the model, the kinetic parameter values in vitro could describe experimental results in vivo when varying each of the PTS protein concentrations individually whilst keeping the other protein concentrations constant. Using the same kinetic constants, but adjusting the protein concentrations in the model to those present in cell-free extracts, the model could reproduce experiments in vitro analysing the dependence of the flux on the total PTS protein concentration. For modeling conditions in vivo it was crucial that the PTS protein concentrations be implemented at their high in vivo values. The model suggests a new interpretation of results hitherto not understood: in vivo, the major fraction of the PTS proteins may exist as complexes with other PTS proteins or boundary metabolites, whereas in vitro, the fraction of complexed proteins is much smaller.
J. Biol. Chem, 10.1074/jbc.M002461200
Submitted on March 23, 2000
Revised on July 5, 2000
Accepted on July 8, 2000
Understanding Glucose Transport by the Bacterial Phosphoenolpyruvate:Glycose Phosphotransferase System on the Basis of Kinetic Measurements in vitro
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