| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print January 8, 2001
Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong
Corresponding Author: mzhang{at}ust.hk
Cytoplasmic dynein is a large, multi-subunit molecular motor that translocates cargoes towards the minus-ends of microtubules. Proper functioning of the dynein motor requires precise assembly of its various subunits. Using purified recombinant proteins, we show that the highly conserved 8 kDa light chain (DLC8) binds to the intermediate chain of the dynein complex. The DLC8-binding region was mapped to a highly conserved 10-residue fragment (amino acid sequence of "SYSKETQTPL") C-terminal to the second alternative splicing site of DIC. Yeast two-hybrid screening using DLC8 as bait identified numerous additional DLC8-binding proteins. Biochemical and mutational analysis of selected DLC8-binding proteins revealed that DLC8 binds to a consensus sequence containing a "K/RXTQT"-motif. The "K/RXTQT"-motif interacts with the common target-accepting grooves of DLC8 dimer. The role of each conserved amino acid residue in this penta-peptide motif in supporting complex formation with DLC8 was systematically studied using site-directed mutagenesis.
J. Biol. Chem, 10.1074/jbc.M010320200
Submitted on November 14, 2000
Revised on January 8, 2001
Accepted on January 8, 2001
The 8 kDa dynein light chain binds to its targets via a conserved "K/R-X-T-Q-T" motif
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Song, W. Wen, S. K. Rayala, M. Chen, J. Ma, M. Zhang, and R. Kumar Serine 88 Phosphorylation of the 8-kDa Dynein Light Chain 1 Is a Molecular Switch for Its Dimerization Status and Functions J. Biol. Chem., February 15, 2008; 283(7): 4004 - 4013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W.-H. Lo, J. M. Kogoy, B. A. Rasoul, S. M. King, and K. K. Pfister Interaction of the DYNLT (TCTEX1/RP3) Light Chains and the Intermediate Chains Reveals Novel Intersubunit Regulation during Assembly of the Dynein Complex J. Biol. Chem., December 21, 2007; 282(51): 36871 - 36878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rompolas, L. B. Pedersen, R. S. Patel-King, and S. M. King Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor J. Cell Sci., October 15, 2007; 120(20): 3653 - 3665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Song, G. Benison, A. Nyarko, T. S. Hays, and E. Barbar Potential Role for Phosphorylation in Differential Regulation of the Assembly of Dynein Light Chains J. Biol. Chem., June 8, 2007; 282(23): 17272 - 17279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Tan, M. A. R. Preuss, J. C. Williams, and M. J. Schnell The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription PNAS, April 24, 2007; 104(17): 7229 - 7234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Marston, L. M. McElhinney, N. Johnson, T. Muller, K. K. Conzelmann, N. Tordo, and A. R. Fooks Comparative analysis of the full genome sequence of European bat lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a conserved transcription termination and polyadenylation motif in the G-L 3' non-translated region J. Gen. Virol., April 1, 2007; 88(4): 1302 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Okamura, C. E. Oki-Idouchi, and P. S. Lorenzo The Exchange Factor and Diacylglycerol Receptor RasGRP3 Interacts with Dynein Light Chain 1 through Its C-terminal Domain J. Biol. Chem., November 24, 2006; 281(47): 36132 - 36139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W.-H. Lo, H.-M. Kan, and K. K. Pfister Identification of a Novel Region of the Cytoplasmic Dynein Intermediate Chain Important for Dimerization in the Absence of the Light Chains J. Biol. Chem., April 7, 2006; 281(14): 9552 - 9559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, T. N. Bui, J. Xiang, and A. Lin Cyclic AMP Inhibits p38 Activation via CREB-Induced Dynein Light Chain Mol. Cell. Biol., February 15, 2006; 26(4): 1223 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.M. K. Mohan, M. Barve, A. Chatterjee, and R. V. Hosur pH driven conformational dynamics and dimer-to-monomer transition in DLC8 Protein Sci., February 1, 2006; 15(2): 335 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ghosh-Roy, B. S. Desai, and K. Ray Dynein Light Chain 1 Regulates Dynamin-mediated F-Actin Assembly during Sperm Individualization in Drosophila Mol. Biol. Cell, July 1, 2005; 16(7): 3107 - 3116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W.-H. Lo, H.-M. Kan, L.-N. Chan, W.-G. Xu, K.-P. Wang, Z. Wu, M. Sheng, and M. Zhang The 8-kDa Dynein Light Chain Binds to p53-binding Protein 1 and Mediates DNA Damage-induced p53 Nuclear Accumulation J. Biol. Chem., March 4, 2005; 280(9): 8172 - 8179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Hendrickson, C. A. Perrone, P. Griffin, K. Wuichet, J. Mueller, P. Yang, M. E. Porter, and W. S. Sale IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending Mol. Biol. Cell, December 1, 2004; 15(12): 5431 - 5442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ghosh-Roy, M. Kulkarni, V. Kumar, S. Shirolikar, and K. Ray Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila Mol. Biol. Cell, May 1, 2004; 15(5): 2470 - 2483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. King, C. L. Brown, K. C. Maier, N. J. Quintyne, and T. A. Schroer Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo Mol. Biol. Cell, December 1, 2003; 14(12): 5089 - 5097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, K. W.-H. Lo, H.-M. Kan, J.-S. Fan, and M. Zhang Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of the Domain-swapped Dimer Assembly J. Biol. Chem., October 17, 2003; 278(42): 41491 - 41499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Petit, M.-L. Giron, J. Tobaly-Tapiero, P. Bittoun, E. Real, Y. Jacob, N. Tordo, H. de The, and A. Saib Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8 J. Cell Sci., August 15, 2003; 116(16): 3433 - 3442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Susalka, K. Nikulina, M. W. Salata, P. S. Vaughan, S. M. King, K. T. Vaughan, and K. K. Pfister The Roadblock Light Chain Binds a Novel Region of the Cytoplasmic Dynein Intermediate Chain J. Biol. Chem., August 30, 2002; 277(36): 32939 - 32946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. King, M. Bonilla, M. E. Rodgers, and T. A. Schroer Subunit organization in cytoplasmic dynein subcomplexes Protein Sci., May 1, 2002; 11(5): 1239 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mebatsion Extensive Attenuation of Rabies Virus by Simultaneously Modifying the Dynein Light Chain Binding Site in the P Protein and Replacing Arg333 in the G Protein J. Virol., December 1, 2001; 75(23): 11496 - 11502. [Abstract] [Full Text]
|
|
|
|
|
|
N. Poisson, E. Real, Y. Gaudin, M.-C. Vaney, S. King, Y. Jacob, N. Tordo, and D. Blondel Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P J. Gen. Virol., November 1, 2001; 82(11): 2691 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Puthalakath, A. Villunger, L. A. O'Reilly, J. G. Beaumont, L. Coultas, R. E. Cheney, D. C. S. Huang, and A. Strasser Bmf: A Proapoptotic BH3-Only Protein Regulated by Interaction with the Myosin V Actin Motor Complex, Activated by Anoikis Science, September 7, 2001; 293(5536): 1829 - 1832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Yang, D. R. Diener, J. L. Rosenbaum, and W. S. Sale Localization of Calmodulin and Dynein Light Chain LC8 in Flagellar Radial Spokes J. Cell Biol., June 11, 2001; 153(6): 1315 - 1326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Mok, K. W.-H. Lo, and M. Zhang Structure of Tctex-1 and Its Interaction with Cytoplasmic Dynein Intermediate Chain J. Biol. Chem., April 20, 2001; 276(17): 14067 - 14074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Vaughan, J. D. Leszyk, and K. T. Vaughan Cytoplasmic Dynein Intermediate Chain Phosphorylation Regulates Binding to Dynactin J. Biol. Chem., July 6, 2001; 276(28): 26171 - 26179. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
