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Papers In Press, published online ahead of print December 19, 2000
Pharmacology, Wayne State University/School of Medicine, Detroit, MI 48201
Corresponding Author: haiwu{at}med.wayne.edu
We have previously demonstrated that sequential activation of the bacterial ilvIH-leuO-leuABCD gene cluster involves a promoter-relay mechanism. In the current study, we show that the final activation of the leuABCD operon is through a transcriptional derepression mechanism. The leuABCD operon is transcriptionally repressed by the presence of a 318 base pair AT-rich upstream element. LeuO is required for derepressing the repressed leuABCD operon. Deletion analysis of the repressive effect of the 318 bp element has led to the identification of a 72 bp AT-rich (78% A+T) DNA sequence element, AT4, which is capable of silencing a number of unrelated promoters in addition to the leuABCD promoter. AT4-mediated gene silencing is orientation-independent and occurs within a distance of 300 base pair. Furthermore, an increased gene silencing effect was observed with a tandemly repeated AT4 dimer. The possible mechanism of AT4-mediated gene silencing in bacteria is discussed.
J. Biol. Chem, 10.1074/jbc.M010501200
Submitted on November 20, 2000
Revised on December 15, 2000
Accepted on December 18, 2000
A 72 Base Pair AT-rich DNA Sequence Element Functions as A Bacterial Gene Silencer
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