JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/23/19820    most recent
M010838200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wade, N.
Right arrow Articles by James, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wade, N.
Right arrow Articles by James, D. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print March 1, 2001
J. Biol. Chem, 10.1074/jbc.M010838200
Submitted on November 30, 2000
Revised on February 23, 2001
Accepted on March 1, 2001

Syntaxin 7 complexes with mVti1b, Syntaxin 6, VAMP8 and VAMP7 in B16 melanoma cells

Nick Wade, Nia J. Bryant, Lisa M. Connolly, Richard J. Simpson, J. Paul Luzio, Robert C. Piper, and David E. James

Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Queensland 4072

Corresponding Author: D.James{at}imb.uq.edu.au

Syntaxin 7 is a mammalian t-SNARE involved in membrane transport between late endosomes and lysosomes. The aim of the present study was to use immunoaffinity techniques to identify proteins that interact with Syntaxin 7. We reasoned that this would be facilitated by the use of cells producing high levels of Syntaxin 7. Screening of a large number of tissues and cell lines revealed that Syntaxin 7 is expressed at very high levels in B16 melanoma cells. Moreover, the expression of Syntaxin 7 increased in these cells as they underwent melanogenesis. From a large scale Syntaxin 7 immunoprecipitation, we have identified six polypeptides using a combination of electrospray mass spectrometry and immunoblotting. These polypeptides corresponded to Syntaxin 7, Syntaxin 6, mVti1b, a-SNAP, VAMP8, VAMP7 and the PP1M phosphatase regulatory subunit. We also observed partial co-localization between Syntaxin 6 and Syntaxin 7, between Syntaxin 6 and mVti1b, but not between Syntaxin 6 and the early endosomal t-SNARE Syntaxin 13. Based on these, and previously reported data, we propose that Syntaxin 7/mVti1b/Syntaxin 6 may form discrete SNARE complexes with either VAMP7 or VAMP8 to regulate fusion events within the late endosomal pathway and that these events may play a critical role in melanogenesis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
H. Mashima, J. Suzuki, T. Hirayama, Y. Yoshikumi, H. Ohno, H. Ohnishi, H. Yasuda, T. Fujita, and M. Omata
Involvement of Vesicle-Associated Membrane Protein 7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-Produced VacA
Infect. Immun., June 1, 2008; 76(6): 2296 - 2303.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. A. Lapierre, K. M. Avant, C. M. Caldwell, A.-J. L. Ham, S. Hill, J. A. Williams, A. J. Smolka, and J. R. Goldenring
Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling
Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1249 - G1262.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. A. Siddiqi, J. Mahan, S. Siddiqi, F. S. Gorelick, and C. M. Mansbach II
Vesicle-associated membrane protein 7 is expressed in intestinal ER.
J. Cell Sci., March 1, 2006; 119(Pt 5): 943 - 950.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. C. Theos, D. Tenza, J. A. Martina, I. Hurbain, A. A. Peden, E. V. Sviderskaya, A. Stewart, M. S. Robinson, D. C. Bennett, D. F. Cutler, et al.
Functions of Adaptor Protein (AP)-3 and AP-1 in Tyrosinase Sorting from Endosomes to Melanosomes
Mol. Biol. Cell, November 1, 2005; 16(11): 5356 - 5372.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Nakamura, H. Fukuda, A. Kato, and S. Hirose
MARCH-II Is a Syntaxin-6-binding Protein Involved in Endosomal Trafficking
Mol. Biol. Cell, April 1, 2005; 16(4): 1696 - 1710.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Z. Murray, F. G. Wylie, T. Khromykh, D. A. Hume, and J. L. Stow
Syntaxin 6 and Vti1b Form a Novel SNARE Complex, Which Is Up-regulated in Activated Macrophages to Facilitate Exocytosis of Tumor Necrosis Factor-{alpha}
J. Biol. Chem., March 18, 2005; 280(11): 10478 - 10483.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Kuliawat, E. Kalinina, J. Bock, L. Fricker, T. E. McGraw, S. R. Kim, J. Zhong, R. Scheller, and P. Arvan
Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic {beta}-Cells
Mol. Biol. Cell, April 1, 2004; 15(4): 1690 - 1701.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. C. W. Richardson, S. C. Winistorfer, V. Poupon, J. P. Luzio, and R. C. Piper
Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton
Mol. Biol. Cell, March 1, 2004; 15(3): 1197 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Alberts, R. Rudge, I. Hinners, A. Muzerelle, S. Martinez-Arca, T. Irinopoulou, V. Marthiens, S. Tooze, F. Rathjen, P. Gaspar, et al.
Cross Talk between Tetanus Neurotoxin-insensitive Vesicle-associated Membrane Protein-mediated Transport and L1-mediated Adhesion
Mol. Biol. Cell, October 1, 2003; 14(10): 4207 - 4220.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Atlashkin, V. Kreykenbohm, E.-L. Eskelinen, D. Wenzel, A. Fayyazi, and G. Fischer von Mollard
Deletion of the SNARE vti1b in Mice Results in the Loss of a Single SNARE Partner, Syntaxin 8
Mol. Cell. Biol., August 1, 2003; 23(15): 5198 - 5207.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. G. Duman and J. G. Forte
What is the role of SNARE proteins in membrane fusion?
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C237 - C249.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Suzuki, H. Ohnishi, A. Wada, T. Hirayama, H. Ohno, N. Ueda, H. Yasuda, T. Iiri, Y. Wada, M. Futai, et al.
Involvement of Syntaxin 7 in Human Gastric Epithelial Cell Vacuolation Induced by the Helicobacter pylori-produced Cytotoxin VacA
J. Biol. Chem., July 3, 2003; 278(28): 25585 - 25590.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Martinez-Arca, V. Proux-Gillardeaux, P. Alberts, D. Louvard, and T. Galli
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles
J. Cell Sci., July 1, 2003; 116(13): 2805 - 2816.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. M. Shewan, E. M. van Dam, S. Martin, T. B. Luen, W. Hong, N. J. Bryant, and D. E. James
GLUT4 Recycles via a trans-Golgi Network (TGN) Subdomain Enriched in Syntaxins 6 and 16 But Not TGN38: Involvement of an Acidic Targeting Motif
Mol. Biol. Cell, March 1, 2003; 14(3): 973 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Hasegawa, S. Zinsser, Y. Rhee, E. O. Vik-Mo, S. Davanger, and J. C. Hay
Mammalian Ykt6 Is a Neuronal SNARE Targeted to a Specialized Compartment by its Profilin-like Amino Terminal Domain
Mol. Biol. Cell, February 1, 2003; 14(2): 698 - 720.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Xu, S. Martin, D. E. James, and W. Hong
GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi Apparatus
Mol. Biol. Cell, October 1, 2002; 13(10): 3493 - 3507.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. S. Misura, J. B. Bock, L. C. Gonzalez Jr., R. H. Scheller, and W. I. Weis
Three-dimensional structure of the amino-terminal domain of syntaxin 6, a SNAP-25 C homolog
PNAS, July 9, 2002; 99(14): 9184 - 9189.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. A. Sanderfoot, V. Kovaleva, D. C. Bassham, and N. V. Raikhel
Interactions between Syntaxins Identify at Least Five SNARE Complexes within the Golgi/Prevacuolar System of the Arabidopsis Cell
Mol. Biol. Cell, December 1, 2001; 12(12): 3733 - 3743.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. S. Misura, L. C. Gonzalez Jr., A. P. May, R. H. Scheller, and W. I. Weis
Crystal Structure and Biophysical Properties of a Complex between the N-terminal SNARE Region of SNAP25 and Syntaxin 1a
J. Biol. Chem., October 26, 2001; 276(44): 41301 - 41309.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.