| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print October 11, 2001
Biochem. & Mol. Biol., Peking Univ. Health Science Center, Beijing, Beijing 100083
Corresponding Author: tanjuntong{at}hotmail.com
Cyclin-dependent kinase inhibitor p16 INK4a is implicated in replicative senescence, cell immortalization and tumor generation. However the mechanism regulating its over-expression in senescent cells is unknown. We used the EGFP reporter system to scan regulatory elements in the upstream region of p16 INK4a . The results of 5¡¦-deletion studies indicated that the transcription regulatory elements contributing to overexpression of p16 INK4a in senescent cells were located in the region of the p16 INK4a promoter from ¡V622bp to ¡V280bp. According to the results of in vitro DNase I footprinting, EMSA, and Southwestern blotting, we found a novel negative regulatory element, ITSE (INK4a transcription silence element), at ¡V491 to ¡V485bp of the p16 INK4a promoter. A 24kDa protein that was highly expressed in young cells may inhibit the expression of p16 INK4a by interacting with ITSE. A GC-abundant element located in the region from ¡V466 to ¡V451bp was also involved with high expression of p16 in senescent fibroblasts.
J. Biol. Chem, 10.1074/jbc.M108278200
Submitted on August 28, 2001
Revised on October 11, 2001
Accepted on October 11, 2001
Characterization of regulatory elements on the promoter region of p16 INK4a that contribute to overexpression of p16 in senescent fibroblasts
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Q. Gan, J. Huang, R. Zhou, J. Niu, X. Zhu, J. Wang, Z. Zhang, and T. Tong PPAR{gamma} accelerates cellular senescence by inducing p16INK4{alpha} expression in human diploid fibroblasts J. Cell Sci., July 1, 2008; 121(13): 2235 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. F. Lu, X. G. Jiang, Y. B. Lu, J. H. Bai, and Z. B. Mao Characterization of a Novel Positive Transcription Regulatory Element That Differentially Regulates the Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Gene in Senescent Cells J. Biol. Chem., June 17, 2005; 280(24): 22606 - 22615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Youn, Y. Koo, I. Ji, and T. H. Ji An Upstream Initiator-Like Element Suppresses Transcription of the Rat Luteinizing Hormone Receptor Gene Mol. Endocrinol., May 1, 2005; 19(5): 1318 - 1328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhao, T. Tong, and Z. Zhang Expression of the Leo1-like domain of replicative senescence down-regulated Leo1-like (RDL) protein promotes senescence of 2BS fibroblasts FASEB J, April 1, 2005; 19(6): 521 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zheng, H. Wang, L. Xue, Z. Zhang, and T. Tong Regulation of Cellular Senescence and p16INK4a Expression by Id1 and E47 Proteins in Human Diploid Fibroblast J. Biol. Chem., July 23, 2004; 279(30): 31524 - 31532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. B. Roninson Tumor Cell Senescence in Cancer Treatment Cancer Res., June 1, 2003; 63(11): 2705 - 2715. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
