| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print May 1, 2002
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118
Corresponding Author: smith{at}biochem.bumc.bu.edu
The transcription start site of the collagen alpha 2(I) gene (COL1A2) has a sequence specific binding site for a DNA methylation responsive binding protein called regulatory factor for X box 1(RFX1) (1). In this manuscript, we demonstrate that RFX1 forms homodimers as well as heterodimers with RFX2 spanning the collagen transcription start site. Methylation at +7 on the coding strand increases RFX1 complex formation in gel shift assays. Methylation on the template strand does not increase RFX1 complex formation. DNA from human fibroblasts contains minimal methylation on the coding strand (<4%) with variable methylation on the template strand. RFX1 acts as a repressor of collagen transcription as judged by in vitro transcription and co-transfection assays with an unmethylated collagen promoter-reporter construct. In addition, an RFX5 complex present in human fibroblasts interacts with the collagen RFX site without sensitivity to methylation. This is the first demonstration of RFX5 complex formation on a gene other than major histocompatibility complex (MHC) promoters. Also, RFX5 represses transcription of a collagen promoter-reporter construct in rat fibroblasts that have no detectable RFX5 complex formation or protein. RFX5 complex activates MHC II transcription by interacting with an interferon-gamma (IFN-gamma) inducible protein, major histocompatibility class II trans-activator (CIITA). Collagen transcription is repressed by IFN-gamma in a dose dependent manner in human but not rat fibroblasts. IFN-gamma enhances RFX5 binding activity, and CIITA is present in the RFX5 complex from IFN-gamma treated human fibroblasts. CIITA repressed collagen gene transcription more effectively in human fibroblasts than rat fibroblasts, suggesting that the RFX5 complex may, in part, recruit CIITA protein to the collagen transcription start site. Thus the RFX family may be important repressors of collagen gene transcription through a RFX binding site spanning the transcription start site.
J. Biol. Chem, 10.1074/jbc.M111712200
Submitted on December 9, 2001
Revised on April 23, 2002
Accepted on May 1, 2002
RFX family interacts at the collagen (COL1A2) start site and represses transcription
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Xu, J. A. Harton, and B. D. Smith CIITA Mediates Interferon-{gamma} Repression of Collagen Transcription through Phosphorylation-dependent Interactions with Co-repressor Molecules J. Biol. Chem., January 18, 2008; 283(3): 1243 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. R. Farmer, and B. D. Smith Peroxisome Proliferator-activated Receptor {gamma} Interacts with CIITA{middle dot}RFX5 Complex to Repress Type I Collagen Gene Expression J. Biol. Chem., September 7, 2007; 282(36): 26046 - 26056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-R. Wang, T. Nemoto, and Y. Yokota RFX1 Mediates the Serum-induced Immediate Early Response of Id2 Gene Expression J. Biol. Chem., September 7, 2007; 282(36): 26167 - 26177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, P. K. Sengupta, E. Seto, and B. D. Smith Regulatory Factor for X-box Family Proteins Differentially Interact with Histone Deacetylases to Repress Collagen {alpha}2(I) Gene (COL1A2) Expression J. Biol. Chem., April 7, 2006; 281(14): 9260 - 9270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fan, M. Zhan, J. Shen, J. L. Martindale, X. Yang, T. Kawai, and M. Gorospe En masse nascent transcription analysis to elucidate regulatory transcription factors Nucleic Acids Res., March 15, 2006; 34(5): 1492 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Buch, B. Polic, B. E. Clausen, S. Weiss, O. Akilli-Ozturk, C.-H. Chang, R. Flavell, A. Schulz, S. Jonjic, A. Waisman, et al. MHC class II expression through a hitherto unknown pathway supports T helper cell-dependent immune responses: implications for MHC class II deficiency Blood, February 15, 2006; 107(4): 1434 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Niesen, A. R. Osborne, H. Yang, S. Rastogi, S. Chellappan, J. Q. Cheng, J. M. Boss, and G. Blanck Activation of a Methylated Promoter Mediated by a Sequence-specific DNA-binding Protein, RFX J. Biol. Chem., November 25, 2005; 280(47): 38914 - 38922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhang and J. C. Reese Molecular Genetic Analysis of the Yeast Repressor Rfx1/Crt1 Reveals a Novel Two-Step Regulatory Mechanism Mol. Cell. Biol., September 1, 2005; 25(17): 7399 - 7411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Whitman, S. Liu, T. Vukosavljevic, L. J. Rush, L. Yu, C. Liu, M. I. Klisovic, K. Maharry, M. Guimond, M. P. Strout, et al. The MLL partial tandem duplication: evidence for recessive gain-of-function in acute myeloid leukemia identifies a novel patient subgroup for molecular-targeted therapy Blood, July 1, 2005; 106(1): 345 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sengupta, Y. Xu, L. Wang, R. Widom, and B. D. Smith Collagen {alpha}1(I) Gene (COL1A1) Is Repressed by RFX Family J. Biol. Chem., June 3, 2005; 280(22): 21004 - 21014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Horvath, W. S. Kistler, and M. K. Kistler RFX2 Is a Potential Transcriptional Regulatory Factor for Histone H1t and Other Genes Expressed During the Meiotic Phase of Spermatogenesis Biol Reprod, November 1, 2004; 71(5): 1551 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, L. Wang, G. Buttice, P. K. Sengupta, and B. D. Smith Major Histocompatibility Class II Transactivator (CIITA) Mediates Repression of Collagen (COL1A2) Transcription by Interferon {gamma} (IFN-{gamma}) J. Biol. Chem., October 1, 2004; 279(40): 41319 - 41332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nozell, Z. Ma, C. Wilson, R. Shah, and E. N. Benveniste Class II Major Histocompatibility Complex Transactivator (CIITA) Inhibits Matrix Metalloproteinase-9 Gene Expression J. Biol. Chem., September 10, 2004; 279(37): 38577 - 38589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bonnafe, M. Touka, A. AitLounis, D. Baas, E. Barras, C. Ucla, A. Moreau, F. Flamant, R. Dubruille, P. Couble, et al. The Transcription Factor RFX3 Directs Nodal Cilium Development and Left-Right Asymmetry Specification Mol. Cell. Biol., May 15, 2004; 24(10): 4417 - 4427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. K. Yee, Y. Yao, P. Li, M. J. Klemsz, J. S. Blum, and C.-H. Chang Cathepsin E: A Novel Target for Regulation by Class II Transactivator J. Immunol., May 1, 2004; 172(9): 5528 - 5534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, L. Wang, G. Buttice, P. K. Sengupta, and B. D. Smith Interferon {gamma} Repression of Collagen (COL1A2) Transcription Is Mediated by the RFX5 Complex J. Biol. Chem., December 5, 2003; 278(49): 49134 - 49144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Higashi, Y. Inagaki, K. Fujimori, A. Nakao, H. Kaneko, and I. Nakatsuka Interferon-{gamma} Interferes with Transforming Growth Factor-{beta} Signaling through Direct Interaction of YB-1 with Smad3 J. Biol. Chem., October 31, 2003; 278(44): 43470 - 43479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Sengupta, E. M. Smith, K. Kim, M. J. Murnane, and B. D. Smith DNA Hypermethylation Near the Transcription Start Site of Collagen {alpha}2(I) Gene Occurs in Both Cancer Cell Lines and Primary Colorectal Cancers Cancer Res., April 15, 2003; 63(8): 1789 - 1797. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
