| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 23, 2002
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755
Corresponding Author: gustav.e.lienhard{at}dartmouth.edu
The insulin receptor substrates (IRS’s) are key components in signaling from the insulin receptor, and consequently any proteins that interact with them are expected to participate in insulin signaling. In this study we have searched for proteins that interact with IRS-4 by identifying the proteins that coimmunoprecipitated with IRS-4 from human embryonic kidney 293 cells by microsequencing through mass spectrometry. A group of proteins was found. These included phosphatidylinositol 3-kinase, a protein previously identified as an IRS-4 interactor, and several proteins for which there was no previous evidence of IRS-4 association. One of these proteins, named IRAS, which had been found earlier in another context, was examined in detail. Results from the overexpression of IRAS, such that its amount was about the same as that of IRS-4, indicated that IRAS associated directly with IRS-4, and showed that the increased complexation of IRS-4 with IRAS did not alter the insulin-stimulated tyrosine phosphorylation of IRS-4 or the association of IRS-4 with phosphatidylinositol 3-kinase or Grb2. On the other hand, overexpression of IRAS enhanced IRS-4 dependent insulin stimulation of the ERK kinases. The domains of IRAS and IRS-4 responsible for the association of these two proteins were identified, and it was shown that IRAS also associates with IRS-1, 2, and 3.
J. Biol. Chem, 10.1074/jbc.M111838200
Submitted on December 12, 2001
Revised on March 12, 2002
Accepted on March 21, 2002
Insulin receptor substrate 4 associates with the protein IRAS
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. O. Hoque, M. S. Kim, K. L. Ostrow, J. Liu, G. B. A. Wisman, H. L. Park, M. L. Poeta, C. Jeronimo, R. Henrique, A. Lendvai, et al. Genome-Wide Promoter Analysis Uncovers Portions of the Cancer Methylome Cancer Res., April 15, 2008; 68(8): 2661 - 2670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wauman, A.-S. De Smet, D. Catteeuw, D. Belsham, and J. Tavernier Insulin Receptor Substrate 4 Couples the Leptin Receptor to Multiple Signaling Pathways Mol. Endocrinol., April 1, 2008; 22(4): 965 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-P. Lim and W. Hong Human Nischarin/Imidazoline Receptor Antisera-selected Protein Is Targeted to the Endosomes by a Combined Action of a PX Domain and a Coiled-coil Region J. Biol. Chem., December 24, 2004; 279(52): 54770 - 54782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hinsby, J. V. Olsen, and M. Mann Tyrosine Phosphoproteomics of Fibroblast Growth Factor Signaling: A ROLE FOR INSULIN RECEPTOR SUBSTRATE-4 J. Biol. Chem., November 5, 2004; 279(45): 46438 - 46447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Mihindukulasuriya, G. Zhou, J. Qin, and T.-H. Tan Protein Phosphatase 4 Interacts with and Down-regulates Insulin Receptor Substrate 4 following Tumor Necrosis Factor-{alpha} Stimulation J. Biol. Chem., November 5, 2004; 279(45): 46588 - 46594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Velliquette and P. Ernsberger The Role of I1-Imidazoline and {alpha}2-Adrenergic Receptors in the Modulation of Glucose Metabolism in the Spontaneously Hypertensive Obese Rat Model of Metabolic Syndrome X J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 646 - 657. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
