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Papers In Press, published online ahead of print July 17, 2002
Cell Biology, University of Virginia Health System, Charlottesville, VA 22908
Corresponding Author: jdc4r{at}virginia.edu
Secretory Carrier Membrane Proteins (SCAMPs) are conserved four transmembrane span proteins associated with recycling vesicular carriers. In mast cells, as in other cell types, SCAMPs 1&2 are present in secretory granule membranes and other intracellular membranes. We now demonstrate a population of these SCAMPs in plasma membranes. While small, this population partially co-localizes with SNARE proteins SNAP-23 and syntaxin 4. A fraction of SCAMPs 1&2 also coimmunoprecipitates with SNAP-23. An oligopeptide, E peptide, within the cytoplasmic segment linking the second and third transmembrane spans, particularly of SCAMP2, potently inhibits exocytosis in streptolysin-O permeabilized mast cells. E peptide is unique to SCAMPs and highly conserved among SCAMP isoforms, and minor changes in its sequence abrogate inhibition. It blocks fusion beyond the putative docking step where granules contact the cell surface and each other during compound exocytosis. Blockade is also beyond Ca2+/ATP-dependent relocation of SNAP-23, which regulates compound exocytosis, and beyond ATP-dependent priming of fusion. Kinetic ordering of exocytotic inhibitors has shown that E peptide acts later than other perturbants at a stage closely associated with membrane fusion. These findings identify a new reagent for analyzing the final stage of exocytosis and point to the likely action of SCAMP2 in this process.
J. Biol. Chem, 10.1074/jbc.M202259200
Submitted on March 7, 2002
Revised on July 15, 2002
Accepted on July 17, 2002
Perturbation of a very late step of regulated exocytosis by a secretory carrier membrane protein (SCAMP2) derived peptide
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