| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print May 6, 2002
Chemistry, Bose Institute, Calcutta, West Bengal 700 009
Corresponding Author: joyoti{at}bosemain.boseinst.ac.in
The recruitment of FtsZ to the septum and its subsequent interaction with other cell division proteins in a spatially and temporally controlled manner are the keys to bacterial cell division. In the present study we have tested the hypothesis that FtsZ and FtsW of Mycobacterium tuberculosis could be binding partners. Using gel renaturation, pull-down and solid phase assays, we confirm that FtsZ and FtsW interact through their C-tails which carry extensions absent in their E. coli counterparts. Crucial to these interactions is the cluster of aspartate residues D367 to D370 of FtsZ which most likely interact with a cluster of positively charged residues in the C-tail of FtsW. Mutations of the aspartate residues 367 to 370 showed that changing three aspartate residues to alanine resulted in complete loss of interaction. This is the first demonstration of the direct interaction between FtsZ and FtsW. We speculate that this interaction between FtsZ and FtsW could serve to anchor FtsZ to the membrane and link septum formation to peptidoglycan synthesis in M. tuberculosis. The findings assume particular significance in view of the global efforts to explore new targets in M. tuberculosis for chemotherapeutic intervention.
J. Biol. Chem, 10.1074/jbc.M203847200
Submitted on April 22, 2002
Revised on May 3, 2002
Accepted on May 5, 2002
Interaction between FtsZ and FtsW of mycobacterium tuberculosis
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Maggi, O. Massidda, G. Luzi, D. Fadda, L. Paolozzi, and P. Ghelardini Division protein interaction web: identification of a phylogenetically conserved common interactome between Streptococcus pneumoniae and Escherichia coli Microbiology, October 1, 2008; 154(10): 3042 - 3052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. V. Mistry, R. Del Sol, C. Wright, K. Findlay, and P. Dyson FtsW Is a Dispensable Cell Division Protein Required for Z-Ring Stabilization during Sporulation Septation in Streptomyces coelicolor J. Bacteriol., August 15, 2008; 190(16): 5555 - 5566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Hett and E. J. Rubin Bacterial Growth and Cell Division: a Mycobacterial Perspective Microbiol. Mol. Biol. Rev., March 1, 2008; 72(1): 126 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Real, A. Fay, A. Eldar, S. M. Pinto, A. O. Henriques, and J. Dworkin Determinants for the Subcellular Localization and Function of a Nonessential SEDS Protein J. Bacteriol., January 1, 2008; 190(1): 363 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thakur and P. K. Chakraborti GTPase Activity of Mycobacterial FtsZ Is Impaired Due to Its Transphosphorylation by the Eukaryotic-type Ser/Thr Kinase, PknA J. Biol. Chem., December 29, 2006; 281(52): 40107 - 40113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osawa and H. P. Erickson FtsZ from Divergent Foreign Bacteria Can Function for Cell Division in Escherichia coli. J. Bacteriol., October 1, 2006; 188(20): 7132 - 7140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Raghunand and W. R. Bishai Mycobacterium smegmatis whmD and its homologue Mycobacterium tuberculosis whiB2 are functionally equivalent. Microbiology, September 1, 2006; 152(Pt 9): 2735 - 2747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chauhan, M. V. V. S. Madiraju, M. Fol, H. Lofton, E. Maloney, R. Reynolds, and M. Rajagopalan Mycobacterium tuberculosis Cells Growing in Macrophages Are Filamentous and Deficient in FtsZ Rings. J. Bacteriol., March 1, 2006; 188(5): 1856 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Del Sol, J. G. L. Mullins, N. Grantcharova, K. Flardh, and P. Dyson Influence of CrgA on Assembly of the Cell Division Protein FtsZ during Development of Streptomyces coelicolor J. Bacteriol., February 15, 2006; 188(4): 1540 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osawa and H. P. Erickson Probing the domain structure of FtsZ by random truncation and insertion of GFP Microbiology, December 1, 2005; 151(12): 4033 - 4043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Addinall, E. Small, D. Whitaker, S. Sturrock, W. D. Donachie, and M. M. Khattar New Temperature-Sensitive Alleles of ftsZ in Escherichia coli J. Bacteriol., January 1, 2005; 187(1): 358 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Borhani and E. L. White Polymerization of C-terminally truncated Mycobacterium tuberculosis FtsZ is unlikely to be physiologically relevant Microbiology, December 1, 2004; 150(12): 3903 - 3906. [Full Text] [PDF]
|
|
|
|
|
|
J. Dziadek, S. A. Rutherford, M. V. Madiraju, M. A. L. Atkinson, and M. Rajagopalan Conditional expression of Mycobacterium smegmatis ftsZ, an essential cell division gene Microbiology, June 1, 2003; 149(6): 1593 - 1603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Errington, R. A. Daniel, and D.-J. Scheffers Cytokinesis in Bacteria Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 52 - 65. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
