| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print August 21, 2002
Med. Biology & Human Genetics, University of Innsbruck, Innsbruck A6020
Corresponding Author: Gottfried.Baier{at}uibk.ac.at
Lipoprotein receptors, such as LRP, have been shown to assemble multiprotein complexes containing intracellular signaling molecules, however in vivo, its signaling function is poorly understood. Using a novel LRP receptor fusion construct, a type I transmembrane protein chimera, termed sIgG-LRP (bearing the intracellular COOH-terminal tail of human LRP as recombinant fusion to a transmembrane region plus the extracellular IgG-Fc domain), we here investigated LRP signal transduction specificity in intact cells. First and similar to activated alpha2-macroglobulin as agonist of endogenous LRP, expression of sIgG-LRP demonstrated significant apoptosis protection. Second and similar to alpha2-macroglobulin-induced endogenous LRP, sIgG-LRP is sufficient to negatively modulate mitogen-induced Elk-1 and cJun (but not NF-kappaB) transcriptional activity. Third, expression of sIgG-LRP also impaired cJun transactivation mediated by constitutive active mutants of Rac-1 and MEKK-1. Fourth and unexpectedly, sIgG-LRP expression was found to be associated with a marked enhancement of mitogen-induced cJun amino-terminal kinase (JNK) activation. Fifth, confocal microscopic examination and subcellular fractionation demonstrated that sIgG-LRP and JNK co-localize in transfected cells. Therefore, sIgG-LRP expression was found to significantly impair activation-induced translocation of JNK into the nucleus. Taken together, we here demonstrate that sIgG-LRP protein sequesters activated JNK into the plasma membrane compartment in intact cells, inhibiting nuclear activation of the JNK-dependent transcription factors Elk-1 and cJun.
J. Biol. Chem, 10.1074/jbc.M204426200
Submitted on May 7, 2002
Revised on August 21, 2002
Accepted on August 21, 2002
Evidence of functional modulation of the MEKK/JNK/cJun signaling cascade by the low-density lipoprotein-receptor related protein (LRP)
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. N. Betts, P. van der Geer, and E. A. Komives Structural and Functional Consequences of Tyrosine Phosphorylation in the LRP1 Cytoplasmic Domain J. Biol. Chem., June 6, 2008; 283(23): 15656 - 15664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gaultier, S. Arandjelovic, S. Niessen, C. D. Overton, M. F. Linton, S. Fazio, W. M. Campana, B. F. Cravatt III, and S. L. Gonias Regulation of tumor necrosis factor receptor-1 and the IKK-NF-{kappa}B pathway by LDL receptor-related protein explains the antiinflammatory activity of this receptor Blood, June 1, 2008; 111(11): 5316 - 5325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chen, G. Bu, Y. Takei, K. Sakamoto, S. Ikematsu, T. Muramatsu, and K. Kadomatsu Midkine and LDL-receptor-related protein 1 contribute to the anchorage-independent cell growth of cancer cells J. Cell Sci., November 15, 2007; 120(22): 4009 - 4015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Montel, A. Gaultier, R. D. Lester, W. M. Campana, and S. L. Gonias The Low-Density Lipoprotein Receptor Related Protein Regulates Cancer Cell Survival and Metastasis Development Cancer Res., October 15, 2007; 67(20): 9817 - 9824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dolmer and P. G. W. Gettins Three Complement-like Repeats Compose the Complete {alpha}2-Macroglobulin Binding Site in the Second Ligand Binding Cluster of the Low Density Lipoprotein Receptor-related Protein J. Biol. Chem., November 10, 2006; 281(45): 34189 - 34196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. BU, J. CAM, and C. ZERBINATTI LRP in Amyloid-beta Production and Metabolism Ann. N.Y. Acad. Sci., November 1, 2006; 1086(1): 35 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. M. Campana, X. Li, N. Dragojlovic, J. Janes, A. Gaultier, and S. L. Gonias The Low-Density Lipoprotein Receptor-Related Protein Is a Pro-Survival Receptor in Schwann Cells: Possible Implications in Peripheral Nerve Injury J. Neurosci., October 25, 2006; 26(43): 11197 - 11207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Saraswathi and A. H. Hasty The role of lipolysis in mediating the proinflammatory effects of very low density lipoproteins in mouse peritoneal macrophages J. Lipid Res., July 1, 2006; 47(7): 1406 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gaultier, A. M. Salicioni, S. Arandjelovic, and S. L. Gonias Regulation of the Composition of the Extracellular Matrix by Low Density Lipoprotein Receptor-related Protein-1: ACTIVITIES BASED ON REGULATION OF mRNA EXPRESSION J. Biol. Chem., March 17, 2006; 281(11): 7332 - 7340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Ling, C.-L. Chen, Y.-H. Huang, I-H. Liu, S. S. Huang, and J. S. Huang Identification and Characterization of the Acidic pH Binding Sites for Growth Regulatory Ligands of Low Density Lipoprotein Receptor-related Protein-1 J. Biol. Chem., September 10, 2004; 279(37): 38736 - 38748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Llorente-Cortes, M. Otero-Vinas, S. Camino-Lopez, O. Llampayas, and L. Badimon Aggregated Low-Density Lipoprotein Uptake Induces Membrane Tissue Factor Procoagulant Activity and Microparticle Release in Human Vascular Smooth Muscle Cells Circulation, July 27, 2004; 110(4): 452 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zilberberg, A. Yaniv, and A. Gazit The Low Density Lipoprotein Receptor-1, LRP1, Interacts with the Human Frizzled-1 (HFz1) and Down-regulates the Canonical Wnt Signaling Pathway J. Biol. Chem., April 23, 2004; 279(17): 17535 - 17542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Salicioni, A. Gaultier, C. Brownlee, M. K. Cheezum, and S. L. Gonias Low Density Lipoprotein Receptor-related Protein-1 Promotes {beta}1 Integrin Maturation and Transport to the Cell Surface J. Biol. Chem., March 12, 2004; 279(11): 10005 - 10012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. HUANG, T.-Y. LING, W.-F. TSENG, Y.-H. HUANG, F.-M. TANG, S. M. LEAL, and J. S. HUANG Cellular growth inhibition by IGFBP-3 and TGF-{beta}1 requires LRP-1 FASEB J, November 1, 2003; 17(14): 2068 - 2081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Gregory, H. Singh, A. S. Perry, and D. J. Prockop The Wnt Signaling Inhibitor Dickkopf-1 Is Required for Reentry into the Cell Cycle of Human Adult Stem Cells from Bone Marrow J. Biol. Chem., July 18, 2003; 278(30): 28067 - 28078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Orr, C. E. Pedraza, M. A. Pallero, C. A. Elzie, S. Goicoechea, D. K. Strickland, and J. E. Murphy-Ullrich Low density lipoprotein receptor-related protein is a calreticulin coreceptor that signals focal adhesion disassembly J. Cell Biol., June 23, 2003; 161(6): 1179 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ma, K. S. Thomas, D. J. Webb, R. Moravec, A. M. Salicioni, W. M. Mars, and S. L. Gonias Regulation of Rac1 activation by the low density lipoprotein receptor-related protein J. Cell Biol., December 23, 2002; 159(6): 1061 - 1070. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
