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Papers In Press, published online ahead of print August 19, 2002
Department of Molecular Genetics, Max-Planck-Institute for Biophysical Chemistry, Goettingen D-37077
Corresponding Author: mkonrad{at}gwdg.de
SUMMARY Mammalian synapse-associated protein SAP97, a structural and functional homolog of Drosophila Dlg, is a membrane-associated guanylate kinase (MAGUK) that is present at pre- and postsynaptic sites as well as in epithelial cell-cell contact sites. It is a multidomain scaffolding protein that shares with other members of the MAGUK protein family a characteristic modular organization composed of three sequential protein interaction motifs known as PDZ domains, followed by a Src homolgy 3 (SH3) domain, and an enzymatically inactive guanylate kinase (GK)-like domain. Specific binding partners are known for each domain, and different modes of intramolecular interactions have been proposed which particularly involve the SH3 and GK domains and the HOOK region located between these two domains. We identified the HOOK region as a specific site for calmodulin binding and studied the dynamics of complex formation of recombinant calmodulin and SAP97 by surface plasmon resonance spectroscopy. Binding of various SAP97 deletion constructs to immobilized calmodulin was strictly calcium-dependent. From the rate constants of association and dissociation we determined an equilibrium dissociation constant Kd of 122 nM for the association of calcium-saturated calmodulin and a SAP97 fragment which encompassed the entire SH3-HOOK-GK module. Comparative structure-based sequence analysis of calmodulin binding regions from various target proteins predicts variable affinities for the interaction of calmodulin with members of the MAGUK protein family. Our findings suggest that calmodulin could regulate the intramolecular interaction between the SH3, HOOK, and GK domains of SAP97.
J. Biol. Chem, 10.1074/jbc.M205618200
Submitted on June 6, 2002
Revised on August 19, 2002
Accepted on August 19, 2002
Formation of complexes between Ca2+-calmodulin and the synapse-associated protein SAP97 requires the SH3 domain-GK domain connecting HOOK region
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