| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print October 17, 2002
Internal Medicine, University of Iowa, Iowa City, IA 52242
Corresponding Author: david-kusner{at}uiowa.edu
Many critical cellular processes, including proliferation, vesicle trafficking, and secretion, are regulated by both phospholipase D (PLD) and the actin microfilament system. Stimulation of human PLD1 results in its association with the detergent-insoluble actin cytoskeleton, but the molecular mechanisms and functional consequences of PLD-actin interactions remain incompletely defined. Biochemical and pharmacologic modulation of actin polymerization resulted in complex bidirectional effects on PLD activity, both in vitro and in vivo. Highly-purified G-actin, inhibited basal and stimulated PLD activity, whereas F-actin produced the opposite effects. Actin-induced modulation of PLD activity was independent of the activating stimulus. The efficacy and potency of actin’s effects were isoform-specific, but broadly-conserved among actin family members. Human bg-actin was only 45% as potent and 40% as efficacious as rabbit skeletal muscle a-actin, whereas its inhibitory profile was similar to the single actin species from the yeast, Saccharomyces cereviseae. Use of actin polymerization-specific reagents indicated that PLD1 binds both monomeric G-actin, as well as actin filaments. These data are consistent with a model in which the physical state of the actin cytoskeleton is a critical determinant of its regulation of PLD activity.
J. Biol. Chem, 10.1074/jbc.M209221200
Submitted on September 9, 2002
Revised on October 15, 2002
Accepted on October 17, 2002
Regulation of phospholipase D activity by Actin. Actin exerts bidirectional modulation of mammalian PLD activity in a polymerization-dependent, isoform-specific manner
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Wolyniak and P. Sundstrom Role of Actin Cytoskeletal Dynamics in Activation of the Cyclic AMP Pathway and HWP1 Gene Expression in Candida albicans Eukaryot. Cell, October 1, 2007; 6(10): 1824 - 1840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Kusner, C. R. Thompson, N. A. Melrose, S. M. Pitson, L. M. Obeid, and S. S. Iyer The Localization and Activity of Sphingosine Kinase 1 Are Coordinately Regulated with Actin Cytoskeletal Dynamics in Macrophages J. Biol. Chem., August 10, 2007; 282(32): 23147 - 23162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Lehman, B. Ledford, M. Di Fulvio, K. Frondorf, L. C. McPhail, and J. Gomez-Cambronero Phospholipase D2-derived phosphatidic acid binds to and activates ribosomal p70 S6 kinase independently of mTOR FASEB J, April 1, 2007; 21(4): 1075 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li and H.-W. Xue Arabidopsis PLD{zeta}2 Regulates Vesicle Trafficking and Is Required for Auxin Response PLANT CELL, January 1, 2007; 19(1): 281 - 295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, L. Gao, L. Blanchoin, and C. J. Staiger Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid Mol. Biol. Cell, April 1, 2006; 17(4): 1946 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Iyer, R. S. Agrawal, C. R. Thompson, S. Thompson, J. A. Barton, and D. J. Kusner Phospholipase D1 Regulates Phagocyte Adhesion J. Immunol., March 15, 2006; 176(6): 3686 - 3696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Diaz, S. Mebarek-Azzam, A. Benzaria, M. Dubois, M. Lagarde, G. Nemoz, and A.-F. Prigent Disruption of Lipid Rafts Stimulates Phospholipase D Activity in Human Lymphocytes: Implication in the Regulation of Immune Function J. Immunol., December 15, 2005; 175(12): 8077 - 8086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ziembicki, R. Tandon, J. R. Schelling, J. R. Sedor, R. T. Miller, and C. Huang Mechanical force-activated phospholipase D is mediated by G{alpha}12/13-Rho and calmodulin-dependent kinase in renal epithelial cells Am J Physiol Renal Physiol, October 1, 2005; 289(4): F826 - F834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Komati, F. Naro, S. Mebarek, V. De Arcangelis, S. Adamo, M. Lagarde, A.-F. Prigent, and G. Nemoz Phospholipase D Is Involved in Myogenic Differentiation through Remodeling of Actin Cytoskeleton Mol. Biol. Cell, March 1, 2005; 16(3): 1232 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Iyer, J. A. Barton, S. Bourgoin{section}, and D. J. Kusner Phospholipases D1 and D2 Coordinately Regulate Macrophage Phagocytosis J. Immunol., August 15, 2004; 173(4): 2615 - 2623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Edwards, D. D. Entz, and M. A. Apicella Gonococcal Phospholipase D Modulates the Expression and Function of Complement Receptor 3 in Primary Cervical Epithelial Cells Infect. Immun., November 1, 2003; 71(11): 6381 - 6391. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
