| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print January 13, 2003
Medicine Dept., Emory University and VAMC, Decatur, GA 30033
Corresponding Author: xfan{at}emory.edu
Nitric oxide (NO) is an important molecule with diverse bio-messenger functions including regulation of gene expression. Transcriptional studies using sensitive luciferase reporter systems have suggested that NO inhibits the promoter activity of a variety of genes. Here we report that NO donors (SNP, Deta/NO and NOR4) decrease luciferase activity in a promoter independent fashion in both viral and eukaryotic promoters, with a reduction to nearly 50% in the presence of 100 mM NO donor. Addition of an SV40 enhancer downstream of the luciferase coding region shifted NO donor inhibition to the right, with inhibition at ~300 mM. In contrast, when studied in a CAT reporter, two promoters indicating inhibition by NO were unaffected. The decrease in luciferase activity was not due to NO suppression of the luciferase enzyme. Real-time PCR data showed that luciferase mRNA half-life decreased by nearly half in the presence of NO donor (from 75 to 45 min). The SV40 enhancer prolonged luciferase mRNA half-life, and somewhat blunted the NO effect. Our data suggest that exogenous NO inhibits luciferase activity in dose dependent manner through decreasing luciferase mRNA stability. Thus, the use of luciferase reporter systems to study transcriptional regulation by NO should be attempted with caution.
J. Biol. Chem, 10.1074/jbc.M209911200
Submitted on September 26, 2002
Revised on December 2, 2002
Accepted on January 13, 2003
Nitric oxide donors inhibit luciferase expression in a promoter independent fashion
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Martinez-Moreno, A. Martinez-Ruiz, A. Alvarez-Barrientos, F. Gavilanes, S. Lamas, and I. Rodriguez-Crespo Nitric Oxide Down-regulates Caveolin-3 Levels through the Interaction with Myogenin, Its Transcription Factor J. Biol. Chem., August 10, 2007; 282(32): 23044 - 23054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rubin, T. C. Murphy, J. Rahnert, H. Song, M. S. Nanes, E. M. Greenfield, H. Jo, and X. Fan Mechanical Inhibition of RANKL Expression Is Regulated by H-Ras-GTPase J. Biol. Chem., January 20, 2006; 281(3): 1412 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Raines, G.-L. Cao, S. Porsuphatana, P. Tsai, G. M. Rosen, and P. Shapiro Nitric Oxide Inhibition of ERK1/2 Activity in Cells Expressing Neuronal Nitric-oxide Synthase J. Biol. Chem., February 6, 2004; 279(6): 3933 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fan, E. Roy, L. Zhu, T. C. Murphy, C. Ackert-Bicknell, C. M. Hart, C. Rosen, M. S. Nanes, and J. Rubin Nitric Oxide Regulates Receptor Activator of Nuclear Factor-{kappa}B Ligand and Osteoprotegerin Expression in Bone Marrow Stromal Cells Endocrinology, February 1, 2004; 145(2): 751 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rubin, T. C. Murphy, L. Zhu, E. Roy, M. S. Nanes, and X. Fan Mechanical Strain Differentially Regulates Endothelial Nitric-oxide Synthase and Receptor Activator of Nuclear {kappa}B Ligand Expression via ERK1/2 MAPK J. Biol. Chem., September 5, 2003; 278(36): 34018 - 34025. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
